Effect of Astragaloside Ⅳ on Proliferation， Migration， and Invasion of Colorectal Cancer HCT116 Cells

HOU Benchao; HE Zhijian; LIU Haiyun; LIN Qianxia; FANG Yongqing; ZHAN Shimeng

doi:10.13422/j.cnki.syfjx.20221728

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Effect of Astragaloside Ⅳ on Proliferation， Migration， and Invasion of Colorectal Cancer HCT116 Cells

更新时间：2023-02-07

- Effect of Astragaloside Ⅳ on Proliferation， Migration， and Invasion of Colorectal Cancer HCT116 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 5, Pages: 144-149(2023)
- 作者机构：
  
  1.南昌大学第一附属医院，南昌 330006
  2.江西省肿瘤医院，南昌 330029
  3.江西中医药大学中医学院，南昌 330004
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20221728
  CLC： R22;R242;R2-031;R285.5
- Published：05 March 2023，
  
  Published Online：30 June 2022，
  
  Received：15 March 2022，
- 稿件说明：
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侯本超,何志坚,刘海云等.黄芪甲苷对结直肠癌HCT116细胞增殖、迁移及侵袭的影响[J].中国实验方剂学杂志,2023,29(05):144-149.

HOU Benchao,HE Zhijian,LIU Haiyun,et al.Effect of Astragaloside Ⅳ on Proliferation， Migration， and Invasion of Colorectal Cancer HCT116 Cells[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(05):144-149.
侯本超,何志坚,刘海云等.黄芪甲苷对结直肠癌HCT116细胞增殖、迁移及侵袭的影响[J].中国实验方剂学杂志,2023,29(05):144-149. DOI： 10.13422/j.cnki.syfjx.20221728.

HOU Benchao,HE Zhijian,LIU Haiyun,et al.Effect of Astragaloside Ⅳ on Proliferation， Migration， and Invasion of Colorectal Cancer HCT116 Cells[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(05):144-149. DOI： 10.13422/j.cnki.syfjx.20221728.

摘要

目的

探究黄芪甲苷对结直肠癌HCT116细胞增殖、迁移及侵袭能力的影响，并探讨其相关分子机制。

方法

将结直肠癌HCT116细胞分为空白组（DMSO）和黄芪甲苷低、中、高质量浓度组（15.7、31.4、62.8 mg·L

-1

）。通过倒置显微镜观察给药后结直肠癌HCT116细胞形态的改变；细胞增殖与活性检测-8（CCK-8）法检测黄芪甲苷处理后细胞活性的改变；细胞划痕实验和Transwell实验观察黄芪甲苷处理后结肠癌HCT116细胞迁移及侵袭能力的变化；蛋白免疫印迹法（Western blot）检测结直肠癌HCT16细胞周期蛋白依靠性激酶抑制剂（p21）、细胞周期蛋白D

（CyclinD

）及B细胞淋巴瘤-2（Bcl-2）、Bcl-2相关X蛋白（Bax）表达水平。

结果

与空白组比较，黄芪甲苷低、中、高质量浓度组细胞生长速度慢，密度明显稀疏，细胞形态不一，细胞核逐渐缩小，细胞质逐渐降解，细胞死亡率高，细胞活力呈浓度依赖性下降，细胞迁移和侵袭能力明显受到抑制（

0.05，

0.01），其抑制率与给药浓度呈正相关；与空白组比较，黄芪甲苷低、中、高质量浓度组促凋亡蛋白Bax表达量逐渐升高，呈浓度依赖性，细胞周期蛋白p21、CyclinD

及抑制凋亡蛋白Bcl-2表达量逐渐降低（

0.05，

0.01），呈浓度依赖性。

结论

黄芪甲苷可抑制结直肠癌HCT116细胞的增殖、迁移和侵袭能力，促进细胞凋亡，从而抑制结直肠癌的发生发展。

Abstract

Objective

To investigate effect of astragaloside Ⅳ on the proliferation， migration， and invasion of colorectal cancer HCT116 cells and the underlying molecular mechanism.

Method

Colorectal cancer HCT116 cells were classified into blank group （DMSO） and low-dose （15.7 mg·L

-1

）， medium-dose （31.4 mg·L

-1

）， and high-dose （62.8 mg·L

-1

） astragaloside Ⅳ groups. After drug treatment， the morphological changes of HCT116 cells were observed under an inverted microscope. Cell viability was detected by cell counting kit-8 （CCK-8） assay， and the migration and invasion of cells were detected based on scratch assay and Transwell assay. The expression of cyclin-dependent kinase inhibitor （p21）， CyclinD

， B-cell lymphoma-2 （Bcl-2）， and Bcl-2-associated X protein （Bax） in the cells was examined by Western blot.

Result

Compared with the blank group， cells in the three astragaloside Ⅳ groups demonstrated slow growth， low density， inconsistent morphology， nuclear shrinkage， degradation of cytoplasm， and high death rate. Moreover， cell viability decreased in a concentration-dependent manner in the astragaloside Ⅳ groups. Cell migration and invasion were inhibited （

0.05，

0.01）， and the inhibition rate was in positive correlation with the concentration of the astragaloside Ⅳ. The expression of pro-apoptotic protein Bax in low-dose， medium-dose and high-dose astragaloside Ⅳ groups increased gradually in a concentration-dependent manner， while the expression of p21， CyclinD

and anti-apoptotic protein Bcl-2 decreased gradually in a concentration-dependent manner compared with those in the blank group （

0.05，

0.01）.

Conclusion

Astragaloside Ⅳ can suppress the proliferation， migration， and invasion of colorectal cancer HCT116 cells and promote the apoptosis， thus inhibiting the occurrence and development of colorectal cancer.

关键词

黄芪甲苷结直肠癌增殖迁移侵袭

Keywords

astragaloside Ⅳcolorectal cancerproliferationmigrationinvasion

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