Effect of Loulianwan on Gut Microbiota in db/db Mice with Type 2 Diabetes Mellitus Based on 16S rRNA Sequencing Technology

PENG Chuan; HU Xuefang; CHEN Zhengtao; DOU Chenyu; YANG Peng; DING Lei; WU Lili; QIN Lingling; LIU Tonghua

doi:10.13422/j.cnki.syfjx.20222107

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Effect of Loulianwan on Gut Microbiota in db/db Mice with Type 2 Diabetes Mellitus Based on 16S rRNA Sequencing Technology

更新时间：2023-05-16

- Effect of Loulianwan on Gut Microbiota in db/db Mice with Type 2 Diabetes Mellitus Based on 16S rRNA Sequencing Technology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 12, Pages: 63-70(2023)
- 作者机构：
  
  1.成都中医药大学临床医学院，成都 610036
  2.北京中医药大学中医养生研究所，北京 100029
  3.陕西中医药大学第一临床医学院西安 711301
  4.北京中医药大学第二临床医学院，北京 100078
  5.北京中医药大学，北京 100029
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20222107
  CLC： R2-0;R22;R285.5;R289;R33
- Published：20 June 2023，
  
  Published Online：31 January 2023，
  
  Received：13 September 2022，
- 稿件说明：
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彭川,胡学芳,陈正涛等.基于16S rRNA技术研究蒌连丸对2型糖尿病db/db小鼠肠道菌群的影响[J].中国实验方剂学杂志,2023,29(12):63-70.

PENG Chuan,HU Xuefang,CHEN Zhengtao,et al.Effect of Loulianwan on Gut Microbiota in db/db Mice with Type 2 Diabetes Mellitus Based on 16S rRNA Sequencing Technology[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(12):63-70.
彭川,胡学芳,陈正涛等.基于16S rRNA技术研究蒌连丸对2型糖尿病db/db小鼠肠道菌群的影响[J].中国实验方剂学杂志,2023,29(12):63-70. DOI： 10.13422/j.cnki.syfjx.20222107.

PENG Chuan,HU Xuefang,CHEN Zhengtao,et al.Effect of Loulianwan on Gut Microbiota in db/db Mice with Type 2 Diabetes Mellitus Based on 16S rRNA Sequencing Technology[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(12):63-70. DOI： 10.13422/j.cnki.syfjx.20222107.

摘要

目的

研究蒌连丸对2型糖尿病db/db小鼠肠道菌群的影响。

方法

取4~5周龄雄性db/m+小鼠为正常组，将同周龄成模雄性db/db小鼠根据血糖、体质量随机分为模型组、二甲双胍组（0.25 g·kg

-1

·d

-1

）及蒌连丸组（13 g·kg

-1

·d

-1

），每组6只药物干预5周，每周记录小鼠体质量、进食水量及空腹血糖（FBG），5周后检测空腹血糖、肝脏甘油三酯（TG）、肝脏总胆固醇（TC）、糖化血清蛋白（GSP）、空腹血清胰岛素（FINS），同时计算胰岛素抵抗指数（HOMA-IR）；收集小鼠肠道内粪便，应用16S rRNA技术检测各组小鼠粪便肠道菌群结构变化。

结果

与正常组比较，模型组体质量、进食水量、FBG、肝脏TG、肝脏TC、GSP、FINS及HOMA-IR显著升高（

0.01）；与模型组比较，蒌连丸组可显著降低db/db小鼠饮水量、FBG、肝脏TG、肝脏TC、GSP、FINS及HOMA-IR（

0.01）；蒌连丸组肠道菌群从门到属水平均发生了变化，有益菌相对丰度增加，有害菌相对丰度减少。其中，嗜黏蛋白阿克曼菌、布劳特氏菌、瘤胃球菌、副拟杆菌丰度显著增加（

0.01）。

结论

蒌连丸可以显著改善2型糖尿db/db小鼠糖脂代谢，其机制可能与增加肠道中嗜黏蛋白阿克曼菌、布劳特氏菌、瘤胃球菌、副拟杆菌丰度有关。

Abstract

Objective

To investigate the effect of Loulianwan on the gut microbiota of db/db mice with type 2 diabetes mellitus （T2DM）.

Method

Male db/m+ mice aged 4-5 weeks were assigned to the normal group， and male db/db model mice of the same age were randomly divided into model group， metformin group （0.25 g·kg

-1

·d

-1

）， and Loulianwan group （13 g·kg

-1

·d

-1

）， with six mice in each group. Drug intervention lasted five weeks. The body weight， water intake， and fasting blood glucose （FBG） of the mice were recorded every week. After five weeks， the FBG， liver triglyceride （TG）， liver total cholesterol （TC）， glycated serum protein （GSP）， and fasting serum insulin （FINS） were detected， and the insulin resistance index （HOMA-IR） was calculated. The feces in the mouse intestines were collected， and the 16S rRNA sequencing technology was used to detect the structural changes in the fecal gut microbiota of mice in each group.

Result

Compared with the normal group， the model group showed increased body weight， water intake， FBG， liver TG， liver TC， GSP， FINS， and HOMA-IR （

0.01）. Compared with the model group， the Loulianwan group showed reduced water intake， FBG， liver TG， liver TC， GSP， FINS， and HOMA-IR （

0.01）. The gut microbiota in the Loulian Lills group changed from phylum to genus level. The relative abundance of beneficial bacteria increased and the relative abundance of harmful bacteria decreased. Among them， the abundance of

Akkermansia muciniphila

，

Blautia

，

Ruminococcus

， and

Parabacteroides

increased （

0.01）.

Conclusion

Loulianwan can significantly improve glucose and lipid metabolism in db/db mice with T2DM， and its mechanism may be related to the increase in the abundance of

Akkermansia muciniphila

，

Blautia

，

Ruminococcus

， and

Parabacteroides

in the intestine.

关键词

蒌连丸2型糖尿病糖脂代谢16S rRNAdb/db小鼠

Keywords

Loulianwantype 2 diabetes mellitusglycolipid metabolism16S rRNA sequencing technologydb/db mice

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