Identification and Expression Analysis of <italic style="font-style: italic">AP2/ERF</italic> Family in Stress Responses of <italic style="font-style: italic">Pinellia ternata</italic> Based on Transcriptome Data

YANG Jinrong; CUI Wanning; ZHANG Yu; BO Chen; CHAO Qiujie; ZHU Yanfang; DUAN Yongbo; XUE Tao; ZHANG Aimin

doi:10.13422/j.cnki.syfjx.20230114

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Identification and Expression Analysis of AP2/ERF Family in Stress Responses of Pinellia ternata Based on Transcriptome Data

更新时间：2023-02-07

- Identification and Expression Analysis of AP2/ERF Family in Stress Responses of Pinellia ternata Based on Transcriptome Data
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 5, Pages: 176-184(2023)
- 作者机构：
  
  淮北师范大学生命科学学院安徽省特色资源植物利用工程实验室，安徽淮北 235000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20230114
  CLC： R284.2;R289;R22;R2-031;R33
- Published：05 March 2023，
  
  Published Online：15 December 2022，
  
  Received：10 October 2022，
- 稿件说明：
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杨金荣,崔婉宁,张瑜等.基于转录组数据的半夏AP2/ERF基因家族鉴定及逆境响应分析[J].中国实验方剂学杂志,2023,29(05):176-184.

YANG Jinrong,CUI Wanning,ZHANG Yu,et al.Identification and Expression Analysis of AP2/ERF Family in Stress Responses of Pinellia ternata Based on Transcriptome Data[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(05):176-184.
杨金荣,崔婉宁,张瑜等.基于转录组数据的半夏AP2/ERF基因家族鉴定及逆境响应分析[J].中国实验方剂学杂志,2023,29(05):176-184. DOI： 10.13422/j.cnki.syfjx.20230114.

YANG Jinrong,CUI Wanning,ZHANG Yu,et al.Identification and Expression Analysis of AP2/ERF Family in Stress Responses of Pinellia ternata Based on Transcriptome Data[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(05):176-184. DOI： 10.13422/j.cnki.syfjx.20230114.

摘要

目的

鉴定并研究半夏AP2/ERF转录因子的功能，为推进半夏品种的遗传改良提供理论依据。

方法

该文基于半夏三代转录组数据鉴定了半夏中

AP2/ERF

家族成员，并对其进行系统的生物信息学分析，同时利用实时荧光定量聚合酶链式反应（Real-time PCR）检测半夏

AP2/ERF

在不同组织及不同胁迫条件下的表达情况。

结果

通过转录组数据共筛选出8个全长

AP2/ERF

转录因子家族成员，归为

AP2、ERF

和

DREB

3个亚家族；半夏

AP2/ERF

的氨基酸数目为251~512个，等电点为5.29~11.72，不稳定指数为45.90~82.41，且主要定位于细胞核中；半夏

AP2/ERF

基因的结构域和Motifs相对保守。组织特异性表达模式分析显示，半夏

AP2/ERF

基因在不同组织部位中具有不同的表达模式，且主要在叶中表达。逆境胁迫应答分析表明，

PtERF1

主要响应NaCl的胁迫诱导；

PtERF2

和

PtERF4

在低温和聚乙二醇（PEG）胁迫下表达量均有较大幅度的上调；

PtERF3

同时响应低温和NaCl两种逆境的诱导；

PtERF5

同时响应高温、低温、NaCl、PEG胁迫诱导；

PtERF7

在高温胁迫下表达量增加；而

PtERF8

在低温胁迫下表达量的增加最为显著。

结论

通过实验初步推测半夏

AP2/ERF

基因可响应逆境胁迫，可能具有调控半夏光合作用、提高根系抗逆能力等潜在功能。

Abstract

Objective

To identify the functions of the AP2/ERF family members in

Pinellia ternata

and promote the genetic improvement of

P. ternata

varieties.

Method

We identified and conducted a systematic bioinformatics analysis of the

AP2/ERF

family member genes in

P. ternata

based on the three generations of transcriptome data. Real-time polymerase Chain reaction　（Real-time） PCR was employed to determine the expression pattern of

AP2/ERF

genes in different tissues and under different stress conditions.

Result

A total of eight full-length

AP2/ERF

family members were identified from the transcriptome data， which were classified into three sub-gene families：

AP2

，

ERF

， and

DREB

. The deduced AP2/ERF proteins in

P. ternata

had the length of 251-512 aa， the theoretical pI of 5.29-11.72， the instability index of 45.90-82.41， subcellular localization in the nucleus， and conserved domains and motifs.

AP2/ERF

genes were expressed in different tissues of

P. ternata

， with high expression levels in the leaf. The stress response experiments showed that

PtERF1

mainly responded to NaCl stress. The expression of

PtERF2

and

PtERF4

was significantly up-regulated under low temperature and polyethylene glycol （PEG）-simulated stress.

PtERF3

responded to both low temperature and NaCl stress. The expression of

PtERF5

was induced by high temperature， low temperature， NaCl and PEG stress. The expression of

PtERF7

was up-regulated under high temperature， while that of

PtERF8

under low temperature.

Conclusion

The

AP2/ERF

genes in

P. ternata

can respond to stress and have the potential functions of regulating photosynthesis and improving root stress resistance.

关键词

半夏AP2/ERF基因家族基因鉴定表达分析

Keywords

Pinellia ternataAP2/ERF gene familygene identificationexpression analysis

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Identification and Expression Analysis of AP2/ERF Family in Stress Responses of Pinellia ternata Based on Transcriptome Data

DOI：10.13422/j.cnki.syfjx.20230114

摘要

Abstract

关键词

Keywords

references