Identification and Bioinformatics Analysis of <italic style="font-style: italic">CYP71</italic> Gene Family of Diploid <italic style="font-style: italic">Perilla frutescens</italic>

LIU Mingru; ZHU Yongping; CHEN Sha

doi:10.13422/j.cnki.syfjx.20230315

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Identification and Bioinformatics Analysis of CYP71 Gene Family of Diploid Perilla frutescens

更新时间：2023-04-28

- Identification and Bioinformatics Analysis of CYP71 Gene Family of Diploid Perilla frutescens
  增强出版
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 11, Pages: 160-170(2023)
- 作者机构：
  
  中国中医科学院中药研究所中药鉴定与安全性评估北京市重点实验室，北京 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20230315
  CLC： R284.2;R289;R287;R22;R2-031;R33;R24
- Published：05 June 2023，
  
  Published Online：10 February 2023，
  
  Received：11 October 2022，
- 稿件说明：
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刘鸣茹,朱永平,陈莎.二倍体紫苏CYP71基因家族的鉴定和生物信息学分析[J].中国实验方剂学杂志,2023,29(11):160-170.

LIU Mingru,ZHU Yongping,CHEN Sha.Identification and Bioinformatics Analysis of CYP71 Gene Family of Diploid Perilla frutescens[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(11):160-170.
刘鸣茹,朱永平,陈莎.二倍体紫苏CYP71基因家族的鉴定和生物信息学分析[J].中国实验方剂学杂志,2023,29(11):160-170. DOI： 10.13422/j.cnki.syfjx.20230315.

LIU Mingru,ZHU Yongping,CHEN Sha.Identification and Bioinformatics Analysis of CYP71 Gene Family of Diploid Perilla frutescens[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(11):160-170. DOI： 10.13422/j.cnki.syfjx.20230315.

摘要

目的

CYP71

是细胞色素P450中

CYP71

簇（

CYP71clan

）中的其中一个家族，在次生代谢产物尤其是萜类化合物的生物合成中起到至关重要的作用。为了更好地了解二倍体紫苏

CYP71

基因家族的特征并预测其功能，该研究利用生物信息学方法对二倍体紫苏

CYP71

基因家族进行鉴定和系统性分析。

方法

在二倍体紫苏

PC99

全基因组的基础上，根据

CYP71

基因家族共有的保守结构域进行筛选，利用美国国家生物技术信息中心（NCBI）、TBtools、MEME、MEGA、Cytoscape等工具对二倍体紫苏

CYP71

基因家族的序列特征、基因结构、染色体定位、系统发育、顺式作用元件等进行分析。

结果

在二倍体紫苏中共鉴定出68个

CYP71

基因，不均匀的分布在34条染色体上，属于2个亚家族，编码的氨基酸数目介于481~530，等电点在5.70~9.03，相对分子质量为54 217.07~60 031.79 Da，含有10个保守基序，富集分析和功能注释结果显示共富集到11个通路和114个类别，功能注释主要集中在生物过程中，在

CYP71

基因的启动子区存在多种顺式作用元件，主要为光响应和茉莉酸甲酯响应元件，蛋白质-蛋白质相互作用分析表明CYP71蛋白具有多种功能比如具有萜烯环化酶活性。

结论

该研究为

CYP71

基因家族的功能研究奠定基础，并为紫苏单萜类化合物的生物合成和定向培育优良品种提供参考。

Abstract

Objective

CYP71

gene family is one of the

CYP71

clans belonging to cytochrome P450， which plays an important role in secondary metabolites， especially terpenoid biosynthesis. To understand the characteristics of

CYP71

family of diploid

Perilla frutescens

and predict its function， this study identified and systematically analyzed the family by bioinformatics.

Method

On the basis of the whole genome of diploid

P. frutescens

PC99

， the conserved domains of

CYP71

family of diploid

P. frutescens

were screened， and the sequence characteristics， gene structure， chromosome location， phylogeny and cis-acting elements were analyzed by National Center for Biotechnology Information （NCBI）， TBtools， MEME， Molecular Evolutionary Genetics Analysis （MEGA）， Cytoscape and other tools.

Result

A total of 68

CYP71

genes were identified from diploid

P. frutescens

， which were unevenly distributed on 34 chromosomes and belonged to two subfamilies. They encoded 481-530 amino acids and contained 10 conserved motifs， with the isoelectric point of 5.70-9.03 and the molecular weight of 54 217.07-60 031.79 Da. The enrichment analysis and functional annotation analysis revealed 11 enriched pathways and 114 categories， and the genes were mainly annotated in biological processes. There were many cis-acting elements in the promoter region of

CYP71

， mainly light-responsive and methyl jasmonate-responsive elements. Protein-protein interaction analysis indicated that CYP71 protein had multiple functions such as terpene cyclase activity.

Conclusion

This study lays a foundation for the functional study of

CYP71

family， and provides a reference for the biosynthesis of monoterpenes in

P. frutescens

and the directional cultivation of excellent varieties.

关键词

紫苏二倍体CYP71基因家族生物信息学功能分析

Keywords

Perilla frutescensdiploidCYP71gene familybioinformaticsfunctional analysis

references

国家药典委员会.中华人民共和国药典：一部［M］.北京：中国医药科技出版社，2020：354.

古建兰，任久强，韦雪娇，等.紫苏不同器官活性成分分布及功能性分析研究进展［J］.北方农业学报，2021，49（5）：118-126.

AHMED H M. Ethnomedicinal ， phytochemical and pharmacological investigations of Perilla frutescens （L.） Britt［J］.Molecules，2019，24：102.

DHYANI A，CHOPRA R，GARG M.A review on nutritional value， functional properties and pharmacological application of Perilla （Perilla Frutescens L.）［J］.Biomed Pharmacol J，2019，12（2）：649-660.

闫钰，易红，张东，等.基于HPLC多成分含量测定的紫苏叶质量标准探讨［J］.中国中药杂志，2021，46（16）：4051-4060.

赵奕栋，孙文豪，陈天源，等.紫苏叶有效成分药理作用研究进展［J］.江苏中医药，2022，54（8）：79-82.

XU L，LI Y，FU Q，et al.Perillaldehyde attenuates cerebral ischemia-reperfusion injury-triggered overexpression of inflammatory cytokines via modulating Akt/JNK pathway in the rat brain cortex［J］.Biochem Biophys Res Commun，2014，454（1）：65-70.

HOU T，NETALA V R，ZHANG H，et al.Perilla frutescens：A rich source of pharmacological active compounds［J］.Molecules，2022，27（11）：3578.

GUERRY-FORCE M L，COGGESHALL J，SNAPPER J，et al.Morphology of noncardiogenic pulmonary edema induced by Perilla ketone in sheep［J］.Am J Pathol，1988，133（2）：285-297.

WILSON B J，GARST J E，LINNABARY R D，et al.Perilla ketone：A potent lung toxin from the mint plant，Perilla frutescens Britton［J］.Science，1977，197（4303）：573-574.

YUBA A，HONDA G，KOEZUKA Y，et al.Genetic analysis of essential oil variants in Perilla frutescens［J］.Biochem Genet，1995，33（9/10）：341-348.

FUJIWARA Y，ITO M.Molecular cloning and characterization of a Perilla frutescens cytochrome P450 enzyme that catalyzes the later steps of perillaldehyde biosynthesis［J］.Phytochemistry，2017，134：26-37.

LUPIEN S，KARP F，WILDUNG M，et al.Regiospecific cytochrome P450 limonene hydroxylases from mint （Mentha） species：cDNA isolation， characterization， and functional expression of（-）-4S-limonene-3-hydroxylase and （-）-4S-limonene-6-hydroxylase［J］.Arch Biochem Biophys，1999，368（1）：181-192.

COLLU G，UNVER N，PELTENBURG-LOOMAN A M，et al.Geraniol 10-hydroxylase， a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis［J］.FEBS Lett，2001，508（2）：215-220.

WERCK-REICHHART D，FEYEREISEN R. Cytochromes P450： A success story［J］.Genome Biol，2000，1（6）：1-9

WEITZEL C，SIMONSEN H T.Cytochrome P450-enzymes involved in the biosynthesis of mono- and sesquiterpenes［J］.Phytochem Rev，2015，14（1）：7-24.

HALLAHAN D L，NUGENT J H，HALLAHAN B J，et al.Interactions of Avocado ［Persea americana） cytochrome P-450 with monoterpenoids［J］.Plant Physiol，1992，98（4）：1290-1297.

KIMURA M，ITO M.Bioconversion of essential oil components of Perilla frutescens by saccharomyces cerevisiae［J］.J Nat Med，2020，74（1）：189-199.

ZHANG Y，SHEN Q，LENG L，et al.Incipient diploidization of the medicinal plant Perilla within 10，000 years［J］.Nat Commun，2021，12（1）：5508.

CHEN C，CHEN H，ZHANG Y，et al.TBtools： An integrative toolkit developed for interactive analyses of big biological data［J］.Mol Plant，2020，13（8）：1194-1202.

LU S，WANG J，CHITSAZ F，et al.CDD/SPARCLE： The conserved domain database in 2020［J］.Nucleic Acids Res，2020，48（D1）：D265-D268.

万志庭，鲁梦，吴沙沙，等.中药火麻仁基原植物大麻MIKC型MADS-box基因家族鉴定与表达分析［J］.药学学报，2021，56（11）：3173-3183.

怀浩，董林林，宁康，等.中药火麻仁基原植物Hsp20基因家族鉴定及表达分析［J］.药学学报，2022，57（4）：1203-1215.

HUSSELSTEIN-MULLER T，SCHALLER H，BENVENISTE P.Molecular cloning and expression in yeast of 2，3-oxidosqualene-triterpenoid cyclases from Arabidopsis thaliana［J］.Plant Mol Biol，2001，45（1）：75-92.

MORITA M，SHIBUYA M，KUSHIRO T，et al.Molecular cloning and functional expression of triterpene synthases from pea ［Pisum sativum new alpha-amyrin-producing enzyme is a multifunctional triterpene synthase［J］.Eur J Biochem，2000，267（12）：3453-3460.

MIALOUNDAMA A S，JADID N，BRUNEL J，et al.Arabidopsis ERG28 tethers the sterol C4-demethylation complex to prevent accumulation of a biosynthetic intermediate that interferes with polar auxin transport［J］.Plant Cell，2013，25（12）：4879-4893.

HERL V，FISCHER G，REVA V A，et al.The VEP1 gene （At4g24220） encodes a short-chain dehydrogenase/reductase with 3-oxo-delta4，5-steroid 5beta-reductase activity in Arabidopsis thaliana L［J］.Biochimie，2009，91（4）：517-525.

JOHNSON K L，JONES B J，BACIC A，et al.The fasciclin-like arabinogalactan proteins of Arabidopsis A multigene family of putative cell adhesion molecules［J］.Plant Physiol，2003，133（4）：1911-1925.

WANG M，OGE L，PEREZ-GARCIA M D，et al.The PUF protein family：Overview on PUF RNA targets，biological functions，and post transcriptional regulation［J］.Int J Mol Sci，2018，19（2）：410.

PIVETTI C D，YEN M R，MILLER S，et al.Two families of mechanosensitive channel proteins［J］.Microbiol Mol Biol Rev，2003，67（1）：66-85.

JEFFARES D C，PENKETT C J，BAHLER J.Rapidly regulated genes are intron poor［J］.Trends Genet，2008，24：375-378.

SONG C，LIU Y，SONG A，et al.The chrysanthemum nankingense genome provides insights into the evolution and diversification of chrysanthemum flowers and medicinal traits［J］.Mol Plant，2018，11（12）：1482-1491.

JIANG T，GUO K，LIU L，et al.Integrated transcriptomic and metabolomic data reveal the flavonoid biosynthesis metabolic pathway in Perilla frutescens （L.） leaves［J］.Sci Rep，2020，10（1）：16207.

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Related Author

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Related Institution

Beijing Key Laboratory of Identification and Safety Evaluation of Chinese Medicine， Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences

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Institute of Botany， Chinese Academy of Sciences

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Identification and Bioinformatics Analysis of CYP71 Gene Family of Diploid Perilla frutescens

DOI：10.13422/j.cnki.syfjx.20230315

摘要

Abstract

关键词

Keywords

references