Effect of <italic style="font-style: italic">Magnolia officinalis</italic> Cortex Before and After Sweating on Distribution of Intestinal Flora and Levels of 5-HT and SP in IBS-C Rats

LIU Fang; ZHANG Ruiyuan; LIU Chang; WANG Xiao; HAO Li; YANG Luping

doi:10.13422/j.cnki.syfjx.20230346

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Effect of Magnolia officinalis Cortex Before and After Sweating on Distribution of Intestinal Flora and Levels of 5-HT and SP in IBS-C Rats

更新时间：2023-06-20

- Effect of Magnolia officinalis Cortex Before and After Sweating on Distribution of Intestinal Flora and Levels of 5-HT and SP in IBS-C Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 14, Pages: 141-149(2023)
- 作者机构：
  
  1.成都中医药大学，成都 611137
  2.四川省中医药科学院中医研究所，成都 610031
  3.华润（雅安）三九药业集团，四川雅安 625000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20230346
  CLC： R22;R28;R94;Q939
- Published：20 July 2023，
  
  Published Online：14 December 2022，
  
  Received：13 October 2022，
- 稿件说明：
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刘芳,张芮苑,刘畅等.发汗前后厚朴对IBS-C大鼠肠道菌群分布及5-HT、SP水平的影响[J].中国实验方剂学杂志,2023,29(14):141-149.

LIU Fang,ZHANG Ruiyuan,LIU Chang,et al.Effect of Magnolia officinalis Cortex Before and After Sweating on Distribution of Intestinal Flora and Levels of 5-HT and SP in IBS-C Rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(14):141-149.
刘芳,张芮苑,刘畅等.发汗前后厚朴对IBS-C大鼠肠道菌群分布及5-HT、SP水平的影响[J].中国实验方剂学杂志,2023,29(14):141-149. DOI： 10.13422/j.cnki.syfjx.20230346.

LIU Fang,ZHANG Ruiyuan,LIU Chang,et al.Effect of Magnolia officinalis Cortex Before and After Sweating on Distribution of Intestinal Flora and Levels of 5-HT and SP in IBS-C Rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(14):141-149. DOI： 10.13422/j.cnki.syfjx.20230346.

摘要

目的

研究发汗前后厚朴对便秘型肠易激综合征（IBS-C）大鼠的作用机制。

方法

采用冰水灌胃法建立IBS-C大鼠模型。将大鼠随机分为空白组、模型组、莫沙必利组（1 mg·kg

-1

）、未发汗厚朴组（以下简称“厚朴组”，10 g·kg

-1

）及发汗厚朴组（10 g·kg

-1

）。观察大鼠体质量、粪便含水量、粪便数量变化；采用16S rRNA测序检测大鼠粪便肠道菌群的结构变化；采用酶联免疫吸附测定法（ELISA）测定大鼠结肠组织中5-羟色胺（5-HT）及P物质（SP）水平。

结果

与模型组比较，给药后莫沙必利组、厚朴组、发汗厚朴组的粪便含水量和粪便数量明显升高（

0.05）。16S rRNA测序结果显示，菌群门水平丰度前4种分别为厚壁菌门、拟杆菌门、螺旋体门、变形菌门，与空白组比较，模型组厚壁菌门相对丰度明显降低（

0.05），螺旋体门相对丰度明显增加（

0.05）；与模型组比较，厚朴组及发汗厚朴组的厚壁菌门、螺旋体门比例趋近于空白组，且发汗厚朴组螺旋体门相对丰度低于厚朴组；在科水平上，菌群丰度前4种分别为乳酸菌科、S24_7、瘤胃球菌科、拟杆菌科，与空白组比较，模型组的乳酸菌科相对丰度明显降低（

0.05），模型大鼠给药后厚朴组和发汗厚朴组的乳酸菌科相对丰度明显提高（

0.05），且两组大鼠菌群结构趋近于空白组；在属水平上，菌群丰度前4种分别为乳酸菌属、Unspecified_

24_7、拟杆菌属、密螺旋体属，与空白组比较，模型组的乳酸菌属相对丰度明显降低（

0.05），密螺旋体属的相对丰度则明显增加（

0.05）；与模型组比较，厚朴组与发汗厚朴组乳酸菌属和密螺旋体属菌群结构比例趋近于空白组，说明发汗前后厚朴均可恢复IBS-C大鼠肠道菌群结构。药理实验结果显示，与模型组比较，莫沙必利组的5-HT含量明显降低（

0.05），厚朴组与发汗厚朴组的5-HT和SP含量均显著升高（

0.01），且发汗厚朴组高于厚朴组。

结论

发汗前后厚朴均可通过改善肠道菌群结构、调节5-HT和SP含量治疗IBS-C。

Abstract

Objective

To investigate the mechanism of

Magnolia officinalis

cortex for constipation-type irritable bowel syndrome（IBS-C） rats before and after sweating.

Method

IBS-C rat model was established by gavage of ice water， and rats were randomly divided into the blank group， model group， mosapride group（1 mg·kg

-1

），

M. officinalis

cortex group（10 g·kg

-1

） and sweated

M. officinalis

cortex group（10 g·kg

-1

）. The changes of body weight， fecal number and fecal water content of rats were observed， 16S rRNA sequencing was used to detect the structural changes of fecal intestinal flora in rats， the levels of 5-hydroxytryptamine（5-HT） and substance P（SP） in colonic tissues of rats were determined by enzyme-linked immunosorbent assay（ELISA）.

Result

Compared with the model group， the fecal water content and fecal number of mosapride group，

M. officinalis

cortex group and sweated

M. officinalis

cortex group were significantly increased（

0.05）. At the phylum level， the top four species of flora abundance were Firmicutes， Bacteroidetes， Spirochaetes and Proteobacteria. Compared with the blank group， the proportion of Firmicutes in the model group was significantly reduced（

0.05）， while the proportion of Spirochaetes was significantly increased（

0.05）. Compared with the model group， the proportion of Firmicutes and Spirochaetes in

M. officinalis

cortex group and sweated

M. officinalis

cortex group tended to be similar to that in the blank group， and the proportion of Spirochaetes in sweated

M. officinalis

cortex group was lower than that of

M. officinalis

cortex group. At the family level， the top four species of flora abundance were Lactobacillaceae， S24_7， Ruminococcaceae， Bacteroidaceae， compared with the blank group， the proportion of Lactobacillaceae in the model group decreased significantly（

0.05）， and its proportion in the

M. officinalis

cortex group and sweated

M. officinalis

cortex group increased significantly after administration（

0.05）， and the flora structure of the two groups tended to be similar to that of the blank group. At the genus level， the top four species of flora abundance were

Lactobacillus

， Unspecified_

24_7，

Bacteroides

and

Treponema

. Compared with the blank group， the proportion of

Lactobacillus

in the model group decreased significantly（

0.05）， while the proportion of

Treponema

increased significantly（

0.05）. Compared with the model group， ratio of bacterial structure of

Lactobacillus

and

Treponema

in the

M. officinalis

cortex group and sweated

M. officinalis

cortex group tended to be similar to those in the blank group， indicating that

M. officinalis

cortex could restore the intestinal microbial structure of IBS-C rats before and after sweating. Compared with the model group， the 5-HT content in mosapride group was significantly reduced（

0.05）， the contents of 5-HT and SP in the

M. officinalis

cortex group and sweated

M. officinalis

cortex group were significantly increased（

0.01）， and the sweated

M. officinalis

cortex group was higher than the

M. officinalis

cortex group.

Conclusion

M. officinalis

cortex can play a therapeutic role on IBS-C rats by regulating 5-HT pathway and intestinal flora structure before and after sweating.

关键词

厚朴发汗便秘型肠易激综合征（IBS-C）肠道菌群5-羟色胺（5-HT）P物质（SP）酶联免疫吸附测定法（ELISA）

Keywords

Magnolia officinalis cortexsweatingconstipation-type irritable bowel syndrome（IBS-C）intestinal flora5-hydroxytryptamine（5-HT）substance P（SP）enzyme-linked immunosorbent assay（ELISA）

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⁰

Effect of Magnolia officinalis Cortex Before and After Sweating on Distribution of Intestinal Flora and Levels of 5-HT and SP in IBS-C Rats

DOI：10.13422/j.cnki.syfjx.20230346

摘要

Abstract

关键词

Keywords

references