iTRAQ-based Proteomic Analysis of Serum Differential Proteins from Henoch-Schönlein Purpura Patients Before and After Qishun Baolier Treatment

WURITU Nashun; BUREN Batu; NAREN Qiqige; JIU Guniang; EERDUN Duleng; WANG Shuanglian; GONG Cuiqin; HASHEN Gaowa; JIN Huizhi; TING Haizi; BAI Wurihan

doi:10.13422/j.cnki.syfjx.20230347

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iTRAQ-based Proteomic Analysis of Serum Differential Proteins from Henoch-Schönlein Purpura Patients Before and After Qishun Baolier Treatment

更新时间：2023-06-20

- iTRAQ-based Proteomic Analysis of Serum Differential Proteins from Henoch-Schönlein Purpura Patients Before and After Qishun Baolier Treatment
  增强出版
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 14, Pages: 105-113(2023)
- 作者机构：
  
  内蒙古民族大学附属医院，内蒙古通辽 028000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20230347
  CLC： R22;R28;R96;Q51
- Received：10 October 2022，
  
  Published Online：15 December 2022，
  
  Published：20 July 2023
- 稿件说明：
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乌日图那顺,布仁巴图,娜仁其其格等.基于iTRAQ技术筛选齐顺保利尔治疗过敏性紫癜前后的血清差异蛋白[J].中国实验方剂学杂志,2023,29(14):105-113.

WURITU Nashun,BUREN Batu,NAREN Qiqige,et al.iTRAQ-based Proteomic Analysis of Serum Differential Proteins from Henoch-Schönlein Purpura Patients Before and After Qishun Baolier Treatment[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(14):105-113.
乌日图那顺,布仁巴图,娜仁其其格等.基于iTRAQ技术筛选齐顺保利尔治疗过敏性紫癜前后的血清差异蛋白[J].中国实验方剂学杂志,2023,29(14):105-113. DOI： 10.13422/j.cnki.syfjx.20230347.

WURITU Nashun,BUREN Batu,NAREN Qiqige,et al.iTRAQ-based Proteomic Analysis of Serum Differential Proteins from Henoch-Schönlein Purpura Patients Before and After Qishun Baolier Treatment[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(14):105-113. DOI： 10.13422/j.cnki.syfjx.20230347.

摘要

目的

过敏性紫癜（HSP）是蒙古族医学治疗的优势病种之一，作为治疗HSP的主要方药，齐顺保利尔（QSBLE）临床效果显著，但其作用机制尚不明晰。基于此，本研究拟筛选患者用药前后血清中差异表达蛋白质，探讨QSBLE治疗HSP的分子作用机制。

方法

以患者自身为对照，搜集6~45岁HSP患者30例，分别在每天12：00和24：00口服QSBLE，依据年龄调整剂量，疗程1周。测定所有入组患者治疗前后的蛋白尿、血尿和皮肤紫癜分布。随机选择10例经QSBLE治疗后临床症状明显缓解的患者的血清开展蛋白质组学分析。采用同位素标记相对和绝对定量（iTRAQ）联合液相色谱-串联质谱法（LC-MS/MS）对HSP患者用药前后血清蛋白进行分析，对差异蛋白进行生物信息学分析并构建蛋白质-蛋白质相互作用（PPI）网络。

结果

从血清中鉴定出378个蛋白，其中差异表达蛋白18个，较治疗前水平上调的蛋白有15个，下调的蛋白有3个。生物信息学分析表明，差异蛋白主要涉及适应性免疫应答、免疫应答、免疫球蛋白产生、吞噬作用等生物过程；以生物过程、通路和蛋白构建PPI网络，免疫球蛋白重常数

1（IGHG1）、免疫球蛋白

链7-43（IGLV7-43）、凝溶胶蛋白（GSN）和60 kDa热休克蛋白（HSPD1）蛋白均参与了适应性免疫应答、免疫球蛋白的产生、白细胞介导的免疫、应激反应的调节、免疫系统过程的调节、创伤反应的调节等生物过程和相关通路，且这些蛋白处于PPI网络的中心位置。

结论

QSBLE可能通过调节IGHG1、IGLV7-43、GSN和HSPD1等关键蛋白的表达影响和免疫相关的生物过程发挥治疗HSP的作用。

Abstract

Objective

Henoch-Schönlein purpura（HSP） is one of the dominant diseases in Mongolian medicine. Qishun Baolier（QSBLE）， as the main prescription for the treatment of HSP， has significant clinical effect， but its mechanism is not yet clear. Baed on this， this study is intended to screen the differentially expressed proteins before and after treatment， and preliminarily explore the molecular mechanism of QSBLE in the treatment of HSP.

Method

Taking oneself as the control， 30 HSP patients aged 6-45 years were collected， and QSBLE was taken orally at 12：00 and 24：00， respectively. The dose was adjusted according to age and the course of treatment was one week. The distribution of proteinuria， hematuria and skin purpura of all patients were determined before and after treatment. The serum samples of 10 patients with clinically significant remission after QSBLE treatment were randomly selected for proteomics. Isobaric tags for relative and absolute quantification（iTRAQ） combined with liquid chromatography tandem mass spectrometry（LC-MS/MS） was used to analyze the proteins in serum of HSP patients before and after treatment， and differential proteins were analyzed bioinformatically and the protein-protein interaction（PPI） networks were constructed.

Result

A total of 378 proteins were identified from serum， including 18 differentially expressed proteins， of which 15 proteins were up-regulated and 3 proteins were down regulated. Bioinformatics showed that the differential proteins were mainly involved in biological processes such as immune response， immunoglobulin production， phagocytosis， adaptive immune response before and after treatment. Biological processes， pathways and proteins were used to construct the PPI network， the proteins represented by immunoglobulin heavy constant

1（IGHG1）， immunoglobulin

-chain 7-43（IGLV7-43）， gelsolin（GSN） and 60 kDa heat shock protein（HSPD1） were involved in biological processes and related pathways such as adaptive immune response， immunoglobulin production， leukocyte-mediated immunity， regulation of stress response， regulation of immune system processes， regulation of trauma response， and these proteins were at the center of the PPI network.

Conclusion

QSBLE may play a role in the treatment of HSP by regulating the expression of IGHG1， IGLV7-43， GSN， HSPD1 and other key proteins to affect immune-related biological processes.

关键词

Keywords

references

GAO R ， NIU X L ， ZHU L L ， et al . iTRAQ quantitative proteomic analysis differentially expressed proteins and signal pathways in Henoch-Schönlein purpura nephritis ［J］. Am J Transl Res ， 2020 ， 12 （ 12 ）： 7908 - 7922 .

JELUSIC M ， SESTAN M ， CIMAZ R ， et al . Different histological classifications for Henoch-Schönlein purpura nephritis：Which one should be used？［J］. Pediatr Rheumatol Online J ， 2019 ， 17 （ 1 ）： 10 .

NARCHI H . Risk of long term renal impairment and duration of follow up recommended for Henoch-Schönlein purpura with normal or minimal urinary findings：A systematic review ［J］. Arch Dis Child ， 2005 ， 90 （ 9 ）： 916 - 920 .

布仁巴图，黄丽艳 . 蒙药血宝丸对过敏性紫癜患儿免疫功能影响的临床研究［J］. 中国民族医药杂志， 2010 ， 16 （ 7 ）： 19 - 21 .

布仁巴图，包秀兰，张丽君，等 . 血宝丸治疗过敏性紫癜103例临床观察［J］. 中国民族医药杂志， 1998 ， 4 （ S1 ）： 11 .

罗蓉，史春，韩燕媚，等 . 基于iTRAQ技术筛选妊娠糖尿病患者血清差异蛋白［J］. 中国优生与遗传杂志， 2022 ， 30 （ 2 ）： 224 - 228 .

于润红，张靖宇，刘玉峰，等 . 基于iTRAQ技术筛选儿童急性早幼粒细胞白血病血清差异蛋白［J］. 中国实验血液学杂志， 2021 ， 29 （ 5 ）： 1462 - 1470 .

朱莹 . 基于iTRAQ技术的尘肺病生物标记物筛选及早期筛查应用分析［D］. 唐山：华北理工大学， 2021 .

MCNALLY A ， MCGREGOR D ， SEARLE M ， et al . Henoch-Schönlein purpura in a renal transplant recipient with prior IgA nephropathy following influenza vaccination ［J］. Clin kidney J ， 2013 ， 6 （ 3 ）： 313 - 315 .

FULOP G A ， RAMIREZ PEREZ F I ， KISS T ， et al . IGF-1 deficiency promotes pathological remodeling of cerebral arteries：A potential mechanism contributing to the pathogenesis of intracerebral hemorrhages in aging ［J］. J GerontolA Biol Sci Med Sci ， 2019 ， 74 （ 4 ）： 446 - 454 .

ZHENG L ， RIEHL T E ， STENSON W F . Regulation of colonic epithelial repair in mice by Toll-like receptors and hyaluronic acid ［J］. Gastroenterology ， 2009 ， 137 （ 6 ）： 2041 - 2051 .

JEONG D ， LEE J S ， PARK S H ， et al . Antiphotoaging and antimelanogenic effects of Penthorum chinense Pursh ethanol extract due to antioxidant- and autophagy-inducing properties ［J］. Oxid Med Cell Longev ， 2019 ， 2019 ： 9679731 .

ROH K ， CHO S ， PARK J H ， et al . Therapeutic effects of hyaluronidase on acquired lymphedema using a newly developed mouse limb model ［J］. Exp Biol Med（Maywood）， 2017 ， 242 （ 6 ）： 584 - 592 .

BUCK M D ， O'SULLIVAN D ， GELTINK R I K ， et al . Mitochondrial dynamics controls T cell fate through metabolic programming ［J］. Cell ， 2016 ， 166 （ 1 ）： 63 - 76 .

SAVIC L J ， SCHOBERT L T ， PETERS D ， et al . Molecular imaging of extracellular tumor pH to reveal effects of locoregional therapy on liver cancer microenvironment ［J］. Clin Cancer Res ， 2020 ， 26 （ 2 ）： 428 - 438 .

MARINKO J T ， HUANG H ， PENN W D ， et al . Folding and misfolding of human membrane proteins in health and disease：From single molecules to cellular proteostasis ［J］. Chem Rev ， 2019 ， 119 （ 9 ）： 5537 - 5606 .

TAYLOR K E ， CHEW M V ， ASHKAR A A ， et al . Novel roles of cytoplasmic ICP0：Proteasome-independent functions of the RING finger are required to block interferon-stimulated gene production but not to promote viral replication ［J］. J Virol ， 2014 ， 88 （ 14 ）： 8091 - 8101 .

胡明 . α -酮戊二酸盐受体1与肾小管功能相互关系的探讨［D］. 北京：军事医学科学院， 2013 .

WU M W ， YUAN H W ， LI X B ， et al . Identification of a five-gene signature and establishment of a prognostic nomogram to predict progression-free interval of papillary thyroid carcinoma ［J］. Front Endocrinol（Lausanne）， 2019 ， 10 ： 790 .

杨璐璐，张君 . TGF- β 1 、TNF- α 与儿童紫癜性肾炎的中西医结合研究［J］. 实用中医内科杂志， 2022 ， 36 （ 4 ）： 29 - 31 .

BELABED M ， MAUVAIS F X ， MASCHALIDI S ， et al . Kinesin-1 regulates antigen cross-presentation through the scission of tubulations from early endosomes in dendritic cells ［J］. Nat Commun ， 2020 ， 11 （ 1 ）： 1817 .

GUIL S ， LONG J C ， CACERES J F . hnRNP A1 relocalization to the stress granules reflects a role in the stress response ［J］. Mol Cell Biol ， 2006 ， 26 （ 15 ）： 5744 - 5758 .

陈尧，王亦男，周宏灏 . 核不均一核糖核蛋白功能与疾病关系的研究进展［J］. 生理科学进展， 2008 ， 39 （ 2 ）： 109 - 113 .

杨帆 . 腹型HSP儿童血清差异蛋白质组学的研究［D］. 长春：吉林大学， 2012 .

NAMKUNG J ， SOHN J H ， CHANG J S ， et al . Increased serum angiopoietin-like 6 ahead of metabolic syndrome in a prospective cohort study ［J］. Diabetes Metab J ， 2019 ， 43 （ 4 ）： 521 - 529 .

郑敬民，朱文杰 . 血管生成素样蛋白的研究进展［J］. 医学研究生学报， 2007 ， 20 （ 11 ）： 1194 - 1197 .

ABDALLA M A ， AHMAD Y H . Promising urinary protein biomarkers for the early detection of hepatocellular carcinoma among high-risk hepatitis C virus egyptian patients ［J］. J Cancer ， 2012 ， 3 ： 390 - 403 .

XIAO C F ， CHEN S ， YUAN M C ， et al . Expression of the 60 kDa and 71 kDa heat shock proteins and presence of antibodies against the 71 kDa heat shock protein in pediatric patients with immune thrombocytopenic purpura ［J］. BMC Blood Disord ， 2004 ， 4 ： 1 .

张超 . 内凝集素基因家族的进化研究［D］. 济南：山东大学， 2013 .

TSUJI S ， UEHORI J ， MATSUMOTO M ， et al . Human intelectin is a novel soluble lectin that recognizes galactofuranose in carbohydrate chains of bacterial cell wall ［J］. J Biol Chem ， 2001 ， 276 （ 26 ）： 23456 - 23463 .

PARK S H ， JANG S ， KIM H K . Gardenia jasminoides extract ameliorates DfE-induced atopic dermatitis in mice through restoration of barrier function and T-helper 2-mediated immune response ［J］. Biomed Pharmacother ， 2022 ， 145 ： 112344 .

叶鸿博 . 石膏及其配伍解热作用的物质基础及机制研究［D］. 长春：长春中医药大学， 2020 .

刘晶晶，陈幸，魏志奇 . 川木通的化学成分及鉴别研究［J］. 天然产物研究与开发， 2010 ， 22 （ 6 ）： 998 - 1000 .

LU L ， QIN A P ， HUANG H B ， et al . Shikonin extracted from medicinal Chinese herbs exerts anti-inflammatory effect via proteasome inhibition ［J］. Eur J Pharmacol ， 2011 ， 658 （ 2/3 ）： 242 - 247 .

LI T ， YAN F G ， WANG R ， et al . Shikonin suppresses human T lymphocyte activation through inhibition of IKK β activity and JNK phosphorylation ［J］. Evid Based Complement Alternat Med ， 2013 ， 2013 ： 379536 .

申佳 . 巴西苏木红素对脑缺血再灌注损伤的作用及血管活性研究［D］. 北京：清华大学， 2007 .

李涛，李姝花，徐春杰，等 . 苏木乙酸乙酯提取物对小鼠外周血白细胞介素-1 β 活性的影响［J］. 吉林中医药， 2009 ， 29 （ 1 ）： 77 - 78 .

王世久，宋丽艳，刘玉兰，等 . 五灵脂乙酸乙酯提取物抗炎作用研究［J］. 沈阳药学院学报， 1994 ， 11 （ 1 ）： 49 - 52 .

耿启彬，黄海龙，翁壮锋，等 . 茜草和茜草炭对正常大鼠凝血-纤溶系统的影响［J］. 中国实验方剂学杂志， 2013 ， 19 （ 13 ）： 279 - 282 .

YANG S L ， LIU F ， ZHANG H ， et al . Immunosuppressive effects of rubidatum in mice ［J］. Acta Pharmacol Sin ， 2000 ， 21 （ 8 ）： 765 - 768 .

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