Effect of Polydatin on Growth，Apoptosis， and ROS/p38 MAPK Signaling Pathway of Myeloma Cells

BAI Yu; XIONG Wenqi; ZHU Chunlong; LI Xiaoqing

doi:10.13422/j.cnki.syfjx.20230393

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Effect of Polydatin on Growth，Apoptosis， and ROS/p38 MAPK Signaling Pathway of Myeloma Cells

更新时间：2023-01-03

- Effect of Polydatin on Growth，Apoptosis， and ROS/p38 MAPK Signaling Pathway of Myeloma Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 3, Pages: 104-109(2023)
- 作者机构：
  
  南昌市第三医院，南昌 330009
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20230393
  CLC： R2-0;R22;R285.5;R33;R730
- Received：21 May 2021，
  
  Published Online：30 May 2022，
  
  Published：05 February 2023
- 稿件说明：
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白羽,熊文祺,朱春龙等.虎杖苷对骨髓瘤细胞的生长、凋亡及ROS/p38 MAPK信号通路作用[J].中国实验方剂学杂志,2023,29(03):104-109.

BAI Yu,XIONG Wenqi,ZHU Chunlong,et al.Effect of Polydatin on Growth，Apoptosis， and ROS/p38 MAPK Signaling Pathway of Myeloma Cells[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(03):104-109.
白羽,熊文祺,朱春龙等.虎杖苷对骨髓瘤细胞的生长、凋亡及ROS/p38 MAPK信号通路作用[J].中国实验方剂学杂志,2023,29(03):104-109. DOI： 10.13422/j.cnki.syfjx.20230393.

BAI Yu,XIONG Wenqi,ZHU Chunlong,et al.Effect of Polydatin on Growth，Apoptosis， and ROS/p38 MAPK Signaling Pathway of Myeloma Cells[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(03):104-109. DOI： 10.13422/j.cnki.syfjx.20230393.

摘要

目的

研究虎杖苷对骨髓瘤细胞生长、凋亡及ROS/p38 MAPK信号通路的作用。

方法

体外培养人多发性骨髓瘤（MM）细胞系U266细胞，通过细胞增殖与活性检测（CCK-8）法检测0、20、40、80、160、200 mg·L

-1

质量浓度虎杖苷对U266细胞生长的影响，计算其半抑制浓度（IC

）。取处于对数生长期的U266细胞，随机分为空白组、虎杖苷低质量浓度（80 mg·L

-1

）组、虎杖苷高质量浓度（160 mg·L

-1

）组、硼替佐米（75 nmol·L

-1

）组，经药物分组处理后，通过CCK-8法测定各组细胞活力；通过流式细胞实验测定各组细胞凋亡率；通过酶联免疫吸附测定法（ELISA）测定各组细胞炎性因子肿瘤坏死因子-

（TNF-

）、白细胞介素-1

（IL-1

）及活性氧（ROS）水平；通过蛋白免疫印迹法检测各组细胞凋亡相关因子胱天蛋白酶-9（Caspase-9）、B细胞淋巴瘤-2（Bcl-2）相关X蛋白（Bax）及p38丝裂原活化蛋白激酶（MAPK）、磷酸化（p）-p38 MAPK蛋白表达水平。

结果

与空白组比较，不同浓度虎杖苷均可抑制U266细胞生长（

0.05），并随浓度升高而作用增强，呈剂量依赖性，IC

为156.54 mg·L

-1

。与空白组比较，虎杖苷低、高质量浓度组和硼替佐米组细胞活力明显降低（

0.05），凋亡率、TNF-

、IL-1

及ROS水平、细胞Caspase-9、Bax蛋白表达水平及p-p38 MAPK/p38 MAPK水平明显增高（

0.05）。与虎杖苷低质量浓度组比较，虎杖苷高质量浓度组和硼替佐米组上述指标改善更明显（

0.05），虎杖苷高质量浓度组和硼替佐米差异无统计学意义。

结论

虎杖苷可激活ROS/p38 MAPK信号通路，促使炎性因子表达，抑制U266细胞生长，促进其凋亡。

Abstract

Objective

To analyze the effects of polydatin on myeloma cell growth，apoptosis， and reactive oxygen species（ROS）/p38 mitogen-activated protein kinase（MAPK） signaling pathway.

Method

Human multiple myeloma （MM） cell line U266 cells were cultured in vitro，and the effects of polydatin at 0，20，40，80，160，200 mg·L

-1

on the growth of U266 cells were detected by cell counting kit-8（CCK-8）assay. The half-maximal inhibitory concentration（IC

）was calculated. U266 cells in the logarithmic growth phase were randomly divided into a control group， low- and high-dose polydatin （80 and 160 mg·L

-1

） groups， and a bortezomib （75 nmol·L

-1

） group. After treatment with corresponding drugs，the cell viability of each group was determined by CCK-8 assay. The apoptosis rate of each group was measured by flow cytometry. The levels of inflammatory factors， such as tumor necrosis factor-

（TNF-

），interleukin-1

（IL-1

）， and ROS in each group were measured by enzyme-linked immunosorbent assay （ELISA）. The protein expression levels of apoptosis-related factors， including cysteine aspartate-specific protease-9（Caspase-9），B-cell lymphoma-2-associated X protein（Bax），p38 MAPK，and phosphorylated （p）-p38 MAPK in each group were detected by Western blot.

Result

Compared with the results in the control group， polydatin of different concentrations could inhibit the growth of U266 cells （

0.05），and the effect was potentiated with the increase in the concentration，with IC

of 156.54 mg·L

-1

. Compared with the control group，the groups with drug treatment showed blunted cell viability （

0.05） and increased apoptosis rate，TNF-

，IL-1

，and ROS levels， protein expression levels of Caspase-9， Bax，and p-p38 MAPK/p38 MAPK （

0.05）. Compared with the low-dose polydatin group， the high-dose polydatin group and the bortezomib group showed improved indicators mentioned above （

0.05）， and there was no significant difference between the high-dose polydatin group and the bortezomib group.

Conclusion

Polydatin can activate the ROS/p38 MAPK signaling pathway，promote the expression of inflammatory factors，inhibit the growth of U266 cells，and promote their apoptosis.

关键词

Keywords

references

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