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Biomechanopharmacology of Chinese Medicine Based on Mechano-ion Channel Piezo1： A Review
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 10, Pages: 235-244(2023)
- 作者机构：
中国中医科学院 中药研究所，北京 100700
- 作者简介：
- 基金信息：
DOI：10.13422/j.cnki.syfjx.20230404
CLC： R2-0;R22;R285.5;R284;R33Published：20 May 2023，
Published Online：31 January 2023，
Received：09 October 2022，
- 稿件说明：
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刘陆,王贻琳,肖顺丽等.基于机械力离子通道Piezo1的中药生物力药理学研究进展[J].中国实验方剂学杂志,2023,29(10):235-244.
LIU Lu,WANG Yilin,XIAO Shunli,et al.Biomechanopharmacology of Chinese Medicine Based on Mechano-ion Channel Piezo1： A Review[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(10):235-244.
刘陆,王贻琳,肖顺丽等.基于机械力离子通道Piezo1的中药生物力药理学研究进展[J].中国实验方剂学杂志,2023,29(10):235-244. DOI： 10.13422/j.cnki.syfjx.20230404.
LIU Lu,WANG Yilin,XIAO Shunli,et al.Biomechanopharmacology of Chinese Medicine Based on Mechano-ion Channel Piezo1： A Review[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(10):235-244. DOI： 10.13422/j.cnki.syfjx.20230404.
摘要
缺血性脑卒中是全球致死和致残的主要原因之一。东汉名医华佗提出“血脉流通，病不得生”的原创预防医学思路，为血脉相关的力学因素与药理学的交叉研究开创了广阔空间。在缺血性脑卒中的发病过程中，力学因素贯穿始终，影响血小板与内皮细胞功能及其分子通讯。近年来血流力学因素对于血栓形成及脑卒中的影响，无论是阐释缺血性脑卒中的发病机制，还是作为潜在的治疗靶标，都受到了越来越多的关注。机械力离子通道Piezo1广泛存在于多种细胞表面，除了受到化学物质及内源性物质的调控，还能够感受不同力学条件，调控通道的开启与关闭，激活下游不同信号通路。Piezol被视为力学与生化信号之间的重要连接。多种中药能够影响Piezo1通道蛋白活性，可能通过Piezo1蛋白防治缺血性脑卒中等血栓性疾病。该文综述了Piezo1通道蛋白在不同力学条件下对内皮细胞、血小板生理病理功能的影响，Piezo1在血栓形成过程中的角色，以及靶向Piezo1通道的中药、化药及内源性物质的效应；为进一步探索活血化瘀中药效应机理及新药研发提供新的思路，并逐步深化生物力药理学研究。
Abstract
Ischemic stroke is one of the leading causes of death and disability worldwide. In Han dynasty， HUA Tuo proposed the original preventive medicine idea that "with good blood circulation， the disease cannot be born"， which opened a broad space for the cross-research of blood-related mechanical factors and pharmacology. In the pathogenesis of ischemic stroke， mechanical factors comprehensively affect the function and crosstalk of platelets and endothelial cells. In recent years， as the well-known effects on thrombosis and stroke， more attention has been paid to hemodynamic factors as the participants involved in pathological mechanisms and potential therapeutic targets of ischemic stroke. The mechanical force ion channel Piezo1 widely exists on the surface of many types of cells. Besides being regulated by chemical and endogenous substances， Piezo1 responds to different mechanical conditions， regulates the opening and closing of channels， and activates different downstream signaling pathways. Piezo1 is now regarded as an important connection between mechanical and biochemical signals. A variety of Chinese medicine can affect the activity of Piezo1 protein， which may prevent and treat thrombotic diseases such as ischemic stroke through Piezo1 protein. In this paper， the effects of Piezo1 protein on the physiological and pathological functions of endothelial cells and platelet under different mechanical conditions and the role of Piezo1 in the process of thrombosis were reviewed， as well as the effects of Chinese medicine， chemical medicine， and endogenous substances targeting Piezo1 channel. These could provide new ideas for further exploring the mechanisms of Chinese medicines in activating blood circulation， developing new drugs， and deepening biomechanical-pharmacology research.
关键词
Piezo1内皮细胞血小板血栓形成生物力药理学
Keywords
Piezo1endothelial cellsplateletsthrombosisbiomechanopharmacology
references
Collaborators GBDS： Global， regional， and national burden of stroke， 1990-2016： A systematic analysis for the Global Burden of Disease Study 2016［J］.Lancet Neurol，2019，18（5）：439-458.
FEIGIN V L，BRAININ M，NORRVING B，et al.World Stroke Organization （WSO）： Global stroke fact sheet 2022［J］.Int J Stroke，2022，17（1）：18-29.
FIEGIN V L，NGUYEN G，CERCY K，et al.Global， regional， and country-specific lifetime risks of stroke， 1990 and 2016［J］.N Engl J Med，2018，379（25）：2429-2437.
刘蕾，游云，廖福龙.剪切诱导血小板聚集机制及其治疗进展［J］.药学学报，2020，55（11）：2501-2509.
BENEDEK A，CERNICA D，MESTER A，et al.Modern concepts in regenerative therapy for ischemic stroke： From stem cells for promoting angiogenesis to 3D-bioprinted scaffolds customized via carotid shear stress analysis［J］.Int J Mol Sci，2019，20（10）：2574.
ZHANG J，KONG X，WANG Z，et al.AMP-activated protein kinase regulates glycocalyx impairment and macrophage recruitment in response to low shear stress［J］.FASEB J，2019，33（6）：7202-7212.
FRAGA-SILVA R A，SEEMAN H，MONTECUCCO F，et al.Apelin-13 treatment enhances the stability of atherosclerotic plaques［J］.Eur J Clin Invest，2018，doi：10.1111/eci.12891http://dx.doi.org/10.1111/eci.12891.
JEONG S K，LEE J Y，ROSENSON R S.Association between ischemic stroke and vascular shear stress in the carotid artery［J］.J Clin Neurol，2014，10（2）：133-139.
LIU Q Y，DUAN Q，FU X H，et al.Wall shear stress can improve prediction accuracy for transient ischemic attack［J］.World J Clin Cases，2019，7（18）：2722-2733.
APAYDIN M，CETINOGLU K.Carotid angle in young stroke［J］.Clin Imaging，2021，70：10-17.
MCFADYEN J D，SCHAFF M，PETER K.Current and future antiplatelet therapies： Emphasis on preserving haemostasis［J］.Nat Rev Cardiol，2018，15（3）：181-191.
SYEDA R，FLORENDO M N，COX C D，et al.Piezo1 channels are inherently mechanosensitive［J］.Cell Rep，2016，17（7）：1739-1746.
RANADE S S，SYEDA R，PATAPOUTIAN A.Mechanically activated ion channels［J］.Neuron，2015，87（6）：1162-1179.
GE J，LI W，ZHAO Q，et al.Architecture of the mammalian mechanosensitive Piezo1 channel［J］.Nature，2015，527（7576）：64-69.
ZHAO Q，WU K，GENG J，et al.Ion permeation and mechanotransduction mechanisms of mechanosensitive Piezo channels［J］.Neuron，2016，89（6）：1248-1263.
ZHAO Q，ZHOU H，CHI S，et al.Structure and mechanogating mechanism of the Piezo1 channel［J］.Nature，2018，554（7693）：487-492.
LÜSCHER T F，DAVIES A，BEER J H，et al.Towards personalized antithrombotic management with drugs and devices across the cardiovascular spectrum［J］.Eur Heart J，2022，43（10）：940-958.
ZHU W，GUO S，HOMILIUS M，et al.PIEZO1 mediates a mechanothrombotic pathway in diabetes［J］.Sci Transl Med，2022，14（626）：eabk1707.
ZHAO W，WEI Z，XIN G，et al.Piezo1 initiates platelet hyperreactivity and accelerates thrombosis in hypertension［J］.J Thromb Haemost，2021，19（12）：3113-3125.
GLUKHOV A V，BALYCHEVA M，SANCHEZ-ALONSO J L，et al.Direct evidence for microdomain-specific localization and remodeling of functional L-type calcium channels in rat and human atrial myocytes［J］.Circulation，2015，132（25）：2372-2384.
LIN Y C，GUO Y R，MIYAGI A，et al.Force-induced conformational changes in PIEZO1［J］.Nature，2019，573（7773）：230-234.
ALBARRÁN-JUÁREZ J，IRING A，WANG S，et al.Piezo1 and G（q）/G（11） promote endothelial inflammation depending on flow pattern and integrin activation［J］.J Exp Med，2018，215（10）：2655-2672.
KIM B，ARANY Z.Editorial commentary：Could shear stress mimetics delay complications in COVID-19？［J］.Trends Cardiovasc Med，2022，32（2）：71-72.
SASTRY S，CUOMO F，MUTHUSAMY J.COVID-19 and thrombosis： The role of hemodynamics［J］.Thromb Res，2022，212：51-57.
RODE B，SHI J，ENDESH N，et al.Piezo1 channels sense whole body physical activity to reset cardiovascular homeostasis and enhance performance［J］.Nat Commun，2017，8（1）：350.
LI J，HOU B，TUMOVA S，et al.Piezo1 integration of vascular architecture with physiological force［J］.Nature，2014，515（7526）：279-282.
FARMER L K，ROLLASON R，WHITCOMB D J，et al.TRPC6 binds to and activates calpain， independent of its channel activity， and regulates podocyte cytoskeleton， cell adhesion， and motility［J］.J Am Soc Nephrol，2019，30（10）：1910-1924.
FONG K P，MOLNAR K S，AGARD N，et al.Cleavage of talin by calpain promotes platelet-mediated fibrin clot contraction［J］.Blood Adv，2021，5（23）：4901-4909.
MCHUGH B J，BUTTERY R，LAD Y，et al.Integrin activation by Fam38A uses a novel mechanism of R-Ras targeting to the endoplasmic reticulum［J］.J Cell Sci，2010，123（Pt 1）：51-61.
MCHUGH B J，MURDOCH A，HASLETT C，et al.Loss of the integrin-activating transmembrane protein Fam38A （Piezo1） promotes a switch to a reduced integrin-dependent mode of cell migration［J］.PLoS One，2012，7（7）：e40346.
NOURSE J L，PATHAK M M.How cells channel their stress： Interplay between Piezo1 and the cytoskeleton［J］.Semin Cell Dev Biol，2017，71：3-12.
DOUGUET D，PATEL A，XU A，et al.Piezo ion channels in cardiovascular mechanobiology［J］.Trends Pharmacol Sci，2019，40（12）：956-970.
ZHANG T，CHI S，JIANG F，et al.A protein interaction mechanism for suppressing the mechanosensitive Piezo channels［J］.Nat Commun，2017，8（1）：1797.
BEECH D J，KALLI A C.Force sensing by piezo channels in cardiovascular health and disease［J］.Arterioscler Thromb Vasc Biol，2019，39（11）：2228-2239.
DAVLOUROS P，XANTHOPOULOU I，MPARAMPOUTIS N，et al.Role of calcium in platelet activation： Novel insights and pharmacological implications［J］.Med Chem，2016，12（2）：131-138.
ILKAN Z，WRIGHT J R，GOODALL A H，et al.Evidence for shear-mediated Ca2+ entry through mechanosensitive cation channels in human platelets and a megakaryocytic cell line［J］.J Biol Chem，2017，292（22）：9204-9217.
LIU L，ZHANG Q，XIAO S，et al.Inhibition of shear-induced platelet aggregation by Xueshuantong via targeting piezo1 channel-mediated Ca2+ signaling pathway［J］.Front Pharmacol，2021，12：606245.
CHOW T W，HELLUMS J D，MOAKE J L，et al.Shear stress-induced von Willebrand factor binding to platelet glycoprotein Ib initiates calcium influx associated with aggregation［J］.Blood，1992，80（1）：113-120.
NESBITT W S，WESTEIN E，TOVAR-LOPEZ F J，et al.A shear gradient-dependent platelet aggregation mechanism drives thrombus formation［J］.Nat Med，2009，15（6）：665-673.
SYEDA R，XU J，DUBIN A E，et al.Chemical activation of the mechanotransduction channel Piezo1［J］.Elife，2015，4：e07369.
LACROIX J J，BOTELLO-SMITH W M，LUO Y.Probing the gating mechanism of the mechanosensitive channel Piezo1 with the small molecule Yoda1［J］.Nat Commun，2018，9（1）：2029.
DAVIES J E，LOPRESTO D，APTA B，et al.Using Yoda-1 to mimic laminar flow in vitro： A tool to simplify drug testing［J］.Biochem Pharmacol，2019，168：473-480.
WANG Y，CHI S，GUO H，et al.A lever-like transduction pathway for long-distance chemical- and mechano-gating of the mechanosensitive Piezo1 channel［J］.Nat Commun，2018，9（1）：1300.
YARISHKIN O，PHUONG T，BAUMANN J M，et al.Piezo1 channels mediate trabecular meshwork mechanotransduction and promote aqueous fluid outflow［J］.J Physiol，2021，599（2）：571-592.
BLYTHE N M，MURAKI K，LUDLOW M J，et al.Mechanically activated Piezo1 channels of cardiac fibroblasts stimulate p38 mitogen-activated protein kinase activity and interleukin-6 secretion［J］.J Biol Chem，2019，294（46）：17395-17408.
GNANASAMBANDAM R，GHATAK C，YASMANN A，et al.GsMTx4： Mechanism of inhibiting mechanosensitive ion channels［J］.Biophys J，2017，112（1）：31-45.
BAE C，SACHS F，GOTTLIEB P A.The mechanosensitive ion channel Piezo1 is inhibited by the peptide GsMTx4［J］.Biochemistry，2011，50（29）：6295-6300.
EVANS E L，CUTHBERTSON K，ENDESH N，et al.Yoda1 analogue （Dooku1） which antagonizes Yoda1-evoked activation of Piezo1 and aortic relaxation［J］.Br J Pharmacol，2018，175（10）：1744-1759.
KANG H，HONG Z，ZHONG M，et al.Piezo1 mediates angiogenesis through activation of MT1-MMP signaling［J］.Am J Physiol Cell Physiol，2019，316（1）：C92-C103.
CORDERO-MORALES J F，VÁSQUEZ V.How lipids contribute to ion channel function， a fat perspective on direct and indirect interactions［J］.Curr Opin Struct Biol，2018，51：92-98.
IVKOVIC S，MAJOR T，MITIC M，et al.Fatty acids as biomodulators of Piezo1 mediated glial mechanosensitivity in Alzheimer's disease［J］.Life Sci，2022，297：120470.
ROMERO L O，MASSEY A E，MATA-DABOIN A D，et al.Dietary fatty acids fine-tune Piezo1 mechanical response［J］.Nat Commun，2019，10（1）：1200.
MURATE M，ABE M，KASAHARA K，et al.Transbilayer distribution of lipids at nano scale［J］.J Cell Sci，2015，128（8）：1627-1638.
TSUCHIYA M，HARA Y，OKUDA M，et al.Cell surface flip-flop of phosphatidylserine is critical for Piezo1-mediated myotube formation［J］.Nat Commun，2018，9（1）：2049.
BORBIRO I，BADHEKA D，ROHACS T.Activation of TRPV1 channels inhibits mechanosensitive Piezo channel activity by depleting membrane phosphoinositides［J］.Sci Signal，2015，8（363）：ra15.
BAE C，SACHS F，GOTTLIEB P A.Protonation of the human PIEZO1 ion channel stabilizes inactivation［J］.J Biol Chem，2015，290（8）：5167-5173.
KUNTZE A，GOETSCH O，FELS B，et al.Protonation of Piezo1 impairs cell-matrix interactions of pancreatic stellate cells［J］.Front Physiol，2020，11：89.
王阶，许军，衷敬柏，等.三七总苷对高黏血症患者血小板活化分子表达和血小板聚集的影响［J］.中国中西医结合杂志，2004，24（4）：312-316.
LIAO F L，LI B.Inhibition of shear-induced platelet aggregation by Chinese herbal medicines［J］.Clin Hemorheol Microcirc，1997，17（4）：315-318.
韩淑娴，陈影，张倩，等.流动条件下血栓通胶囊抗血小板黏附的分子药理学机制研究［J］.中国中药杂志，2017，42（2）：341-346.
HAN S，CHEN Y，WANG J，et al.Anti-thrombosis effects and mechanisms by Xueshuantong capsule under different flow conditions［J］.Front Pharmacol，2019，10：35.
ZHANG L，LI Y，MA X，et al.Ginsenoside Rg1-notoginsenoside R1-protocatechuic aldehyde reduces atherosclerosis and attenuates low-shear stress-induced vascular endothelial cell dysfunction［J］.Front Pharmacol，2020，11：588259.
LIU S，PAN X，CHENG W，et al.Tubeimoside Ⅰ antagonizes Yoda1-evoked Piezo1 channel activation［J］.Front Pharmacol，2020，11：768.
PAN X，WAN R，WANG Y，et al.Inhibition of chemically and mechanically activated Piezo1 channels as a mechanism for ameliorating atherosclerosis with salvianolic acid B［J］.Br J Pharmacol，2022，179（14）：3778-3814.
毛晨晗，祁晓霞，张蒙，等.黄芪丹参含药血清调控机械敏感性阳离子通道Piezo1对低流体剪切力诱导血管平滑肌细胞增殖迁移的影响［J］.时珍国医国药，2021，32（11）：2597-2601.
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