GC-MS Analysis of Effect of Different Processing Conditions on Irritation of Magnoliae Officinalis<italic style="font-style: italic"> </italic>Cortex

CAI Pingjun; QIANG Mengqin; XIA Yangmiao; JIA Yaoxia; MENG Xiangqi; LEI Jinjie; LIU Fang; YU Lingying

doi:10.13422/j.cnki.syfjx.20230665

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GC-MS Analysis of Effect of Different Processing Conditions on Irritation of Magnoliae Officinalis Cortex

更新时间：2023-07-18

- GC-MS Analysis of Effect of Different Processing Conditions on Irritation of Magnoliae Officinalis Cortex
  增强出版
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 16, Pages: 197-206(2023)
- 作者机构：
  
  成都中医药大学西南特色中药资源重点实验室，成都611137
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20230665
  CLC： R22;R943.1;R28;O657
- Published：20 August 2023，
  
  Published Online：09 March 2023，
  
  Received：25 November 2022，
- 稿件说明：
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蔡平君,强梦琴,夏阳淼等.不同炮制条件对厚朴刺激性影响的GC-MS分析[J].中国实验方剂学杂志,2023,29(16):197-206.

CAI Pingjun,QIANG Mengqin,XIA Yangmiao,et al.GC-MS Analysis of Effect of Different Processing Conditions on Irritation of Magnoliae OfficinalisCortex[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(16):197-206.
蔡平君,强梦琴,夏阳淼等.不同炮制条件对厚朴刺激性影响的GC-MS分析[J].中国实验方剂学杂志,2023,29(16):197-206. DOI： 10.13422/j.cnki.syfjx.20230665.

CAI Pingjun,QIANG Mengqin,XIA Yangmiao,et al.GC-MS Analysis of Effect of Different Processing Conditions on Irritation of Magnoliae OfficinalisCortex[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(16):197-206. DOI： 10.13422/j.cnki.syfjx.20230665.

摘要

目的

通过比较厚朴及其不同炮制品中挥发油的刺激性大小及其成分组成，探究对刺激性产生影响的关键化合物并明确炮制过程中加热及辅料姜汁的加入对刺激性的影响。

方法

通过水蒸气蒸馏法提取厚朴、水炙厚朴、姜厚朴（烘法）、姜炙厚朴（10%、50%）等样品的挥发油并进行家兔眼刺激实验，采用气相色谱-质谱法（GC-MS）检测各样品的挥发性成分。运用SIMCA 14.1对各样品数据进行主成分分析（PCA）和正交偏最小二乘法判别分析（OPLS-DA），将不同炮制品与生厚朴或姜炙厚朴（10%、50%）的相对质量分数两两比较，根据变量重要性投影（VIP）值

1、

0.05的原则筛选出差异性标志物，并分析刺激性差异的影响因素。

结果

与空白组比较，给药组对家兔眼睛均有刺激性，刺激性强弱顺序为厚朴

水炙厚朴

姜厚朴（烘法）

姜炙厚朴（10%）

姜炙厚朴（50%）。PCA及OPLS-DA结果表明厚朴及不同炮制品的挥发油存在差异，根据VIP

1、

0.05，结合眼刺激实验结果，筛选出10种与刺激性变化相关的挥发性成分。其中反式肉桂醛仅在厚朴中检出；

-石竹烯、

-杜松烯、

-律草烯、

-衣兰油烯、（-）-异喇叭烯、柠檬醛相对质量分数均有不同程度的增加；4-异丙基甲苯、1（10）-4-杜松萜二烯-15-醇、

-桉叶醇相对质量分数均有不同程度的减少。

结论

厚朴经加热与辅料姜汁炮制后刺激性均降低，且二者的协同作用对于刺激性的减少更有效。与刺激性变化相关的差异性标志物中，其中柠檬醛相对质量分数的增加与姜汁有关，反式肉桂醛、4-异丙基甲苯、1（10）-4-杜松萜二烯-15-醇相对质量分数的减少与加热有关，其他成分的变化可能与加热和姜汁的协同作用有关。

Abstract

Objective

To investigate the key compounds affecting the irritation and to clarify the effect of heating and the addition of ginger juice as the auxiliary material during the processing on the irritation of Magnoliae Officinalis

Cortex（MOC） by comparing the irritation and composition of volatile oil in MOC and its different processed products.

Method

Volatile oil in raw products， water-processed products

，

ginger-dried products， ginger-fried products（the amounts of ginger were 10%， 50%， respectively） of MOC were extracted by steam distillation and subjected to rabbit eye irritation experiment， and the volatile components of each sample were detected by gas chromatography-mass spectrometry（GC-MS）. Principal component analysis（PCA）and orthogonal partial least squares-discriminant analysis（OPLS-DA） were used to analyze the data of each sample by SIMCA 14.1. The relative contents of different processed products were compared two by two with those of and raw products or ginger-fried products， and the markers that might be related to the irritation were sorted out according to the principles of variable importance in the projection（VIP） value

1 and

0.05， and the factors influencing the differences in irritation were analyzed.

Result

Compared with the blank group， the administration groups all had irritation to the eyes of rabbits， and the degree of irritation was in the order of raw products

water-processed products

ginger-dried products

ginger-fried products（10%）

ginger-fried products（50%）. The results of PCA and OPLS-DA showed that there were differences in the

volatile oil from raw products and different processed products. According to VIP value

1 and

0.05， and combined with the results of eye irritation experiment， ten volatile compounds related to irritation changes were screened out. Among them，

cis

-cinnamaldehyde was only detected in raw products， the relative contents of

-caryophyllene，（+）-delta-cadinene，

-humulene，

-muurolene，（-）-isoledene and citral all increased to different degrees， the contents of

-cymene， 1（10）-4-cadinadien-15-ol and

-eudesmol all decreased to different degrees.

Conclusion

The irritation of MOC is reduced after heating and processing with ginger juice， and the synergistic effect of both is more effective for reducing irritation. Among the differential markers associated with changes in irritation， the increase in the relative content of citral is closely related to the addition of ginger juice， while the decrease in the relative contents of

cis

-cinnamaldehyde，

-cymene， 1（10）-4-cadinadien-15-ol is related to heating， and the changes of other components may be related to the synergistic effect of heating and ginger juice.

关键词

厚朴炮制姜制挥发油刺激性气相色谱-质谱法（GC-MS）多元统计分析

Keywords

Magnoliae Officinalis Cortexprocessingprocessed with gingervolatile oilirritationgas chromatography-mass spectrometry（GC-MS）multivariate statistical analysis

references

国家药典委员会.中华人民共和国药典：一部［M］.北京：中国医药科技出版社，2020：263.

谭珍媛，邓家刚，张彤，等.中药厚朴现代药理研究进展［J］.中国实验方剂学杂志，2020，26（22）：228-234.

钱锦秀，孟武威，刘晖晖，等.经典名方中厚朴的本草考证［J］.中国实验方剂学杂志，2022，28（10）：306-317.

张淑洁，钟凌云.厚朴化学成分及其现代药理研究进展［J］.中药材，2013，36（5）：838-843.

胡慧芬，陈斯，翁德会，等.厚朴正丁醇提取部位药效活性及咽喉刺激性研究［J］.山东化工，2018，47（24）：81-83，86.

刘盈萍.厚朴的主要成分及其药理作用研究概况［J］.当代化工研究，2017（5）：141-142.

钟凌云，兰智慧，祝婧，等.姜制前后厚朴毒性及刺激性作用研究［J］.中成药，2013，35（8）：1782-1785.

荆文光，张权，程显隆，等.基于电子鼻、电子舌技术的姜厚朴炮制机理探讨［J］.河北工业科技，2021，38（5）：414-422.

钟凌云，张淑洁，龚千锋，等.生姜、干姜炮制对厚朴挥发性成分影响比较［J］.中国实验方剂学杂志，2015，21（20）：49-54.

李凤，练志辉，张浩怡，等.厚朴不同炮制品中挥发性成分比较［J］.西部中医药，2020，33（7）：33-38.

袁金凤，乔艺涵，彭诗涛，等.厚朴及其不同炮制品中挥发性成分GC-MS分析［J］.中成药，2019，41（6）：1354-1357.

谭珍媛，邓家刚，张彤，等.中药厚朴现代药理研究进展［J］.中国实验方剂学杂志，2020，26（22）：228-234.

国家药典委员会.中华人民共和国药典：四部［M］.北京：中国医药科技出版社，2020：251.

许锦涛，郑家勤，王晗毓，等.陈皮油微乳处方的优化［J］.中成药，2022，44（7）：2262-2266.

王麟，刘静毅，袁珂，等.姜炙厚朴闷润条件优选［J］.时珍国医国药，2009，20（12）：3094-3096.

阴美华，袁蒙蒙，唐志书，等.姜厚朴的微波炮制工艺的响应面法优化［J］.时珍国医国药，2021，32（10）：2404-2407.

刘娟汝，刘雨诗，刘红梅，等.川芎不同部位及不同加工方法的川芎饮片中挥发油含量及化学成分对比［J］.中国实验方剂学杂志，2020，26（5）：101-107.

孟杰，张村，王礼均，等.不同干燥方式对鱼腥草中挥发性成分的影响［J］.华西药学杂志，2022，37（4）：398-408.

姜思奇，蔡广知，赫一鸣，等.北细辛饮片炮制工艺与药材质量相关性研究［J］.特产研究，2019，41（1）：17-21，31.

BACHIEGA T F，SFORCIN J M.Lemongrass and citral effect on cytokines production by murine macrophages［J］.J Ethnopharmacol，2011，137（1）：909-913.

KATSUKAWA M，NAKATA R，TAKIZAWA Y，et al.Citral， a component of lemongrass oil， activates PPARα and γ and suppresses COX-2 expression［J］.Biochim Biophys Acta，2010，1801（11）：1214-1220.

李潮俊，何鑫柱，陈凯，等.花椒挥发油的生物活性及提取方法研究进展［J］.农产品加工，2022（17）：102-106.

陈宁，黄泽愉，孙晓，等.应用小鼠局部淋巴结试验（LLNA：DA）对13种化学物刺激性和致敏性的评价［J］.毒理学杂志，2022，36（3）：249-253，257.

JIN H，LENG Q，ZHANG C，et al.P-cymene prevent high-fat diet-associated colorectal cancer by improving the structure of intestinal flora［J］.J Cancer，2021，12（14）：4355-4361.

刘晓宇，陈旭冰，陈光勇.β-石竹烯及其衍生物的生物活性与合成研究进展［J］.林产化学与工业，2012，32（1）：104-110.

SCANDIFFIO R，GEDDO F，COTTONE E.Protective effects of （E）-β-caryophyllene（BCP）in chronic inflammation［J］.Nutrients，2020，12（11）：3273.

柴桂芳.基于网络药理学厚朴姜炙的科学内涵研究［J］.海峡药学，2022，34（9）：12-18.

HARO-GONZÁLEZ J N，CASTILLO-HERRERA G A，MARTÍNEZ-VELÁZQUEZ M，et al.Clove essential oil （Syzygium aromaticum L. Myrtaceae）：Extraction，chemical composition，food applications，and essential bioactivity for human health［J］.Molecules，2021，26（21）：6387.

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GC-MS Analysis of Effect of Different Processing Conditions on Irritation of Magnoliae Officinalis Cortex

DOI：10.13422/j.cnki.syfjx.20230665

摘要

Abstract

关键词

Keywords

references