Traditional Chinese Medicine Treats Chronic Atrophic Gastritis via Multiple Pathways

WANG Sici; LI Yuan; LI Ping; ZHENG Haocheng; CHEN Weiyu; DING Xia

doi:10.13422/j.cnki.syfjx.20230813

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Traditional Chinese Medicine Treats Chronic Atrophic Gastritis via Multiple Pathways

更新时间：2023-09-08

- Traditional Chinese Medicine Treats Chronic Atrophic Gastritis via Multiple Pathways
  增强出版
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 19, Pages: 193-202(2023)
- 作者机构：
  
  1.北京中医药大学东直门医院，北京 100700
  2.北京中医药大学国家中医体质与治未病研究院，北京 100029
  3.北京中医药大学中医学院，北京 100029
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20230813
  CLC： R284.2;R285;R289;R287;R22;R2-031;R33;R24
- Published：05 October 2023，
  
  Published Online：27 June 2023，
  
  Received：13 January 2023，
- 稿件说明：
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王思慈,李园,李萍等.中医药治疗慢性萎缩性胃炎的多途径作用机制[J].中国实验方剂学杂志,2023,29(19):193-202.

WANG Sici,LI Yuan,LI Ping,et al.Traditional Chinese Medicine Treats Chronic Atrophic Gastritis via Multiple Pathways[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(19):193-202.
王思慈,李园,李萍等.中医药治疗慢性萎缩性胃炎的多途径作用机制[J].中国实验方剂学杂志,2023,29(19):193-202. DOI： 10.13422/j.cnki.syfjx.20230813.

WANG Sici,LI Yuan,LI Ping,et al.Traditional Chinese Medicine Treats Chronic Atrophic Gastritis via Multiple Pathways[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(19):193-202. DOI： 10.13422/j.cnki.syfjx.20230813.

摘要

目的

基于数据挖掘、系统药理学、分子对接系列研究方法，针对中医药治疗慢性萎缩性胃炎（CAG）的有效专利复方进行用药规律挖掘，进而预测不同功效中药治疗CAG的潜在作用靶标及分子机制。

方法

通过国家知识产权局中国专利系统提取干预CAG的中药复方，综合文献利用中药系统药理学数据库与分析平台（TCMSP）、中医药综合数据库（TCMID）、UniProt数据库获取重点功效药物有效成分与靶点；基于GeneCards、DisGeNet、在线人类孟德尔遗传数据库（OMIM）、MalaCards、京都基因与基因组百科全书（KEGG）、Reactome数据库获取CAG潜在靶点与通路，通过R Studio 4.1.2实现基因本体论（GO）注释和KEGG富集分析；基于STRING 11.0数据库构建蛋白质-蛋白质相互作用网络（PPI）；采用分子对接软件AutoDock Vina 4.2.6对关键靶点和成分进行对接验证。

结果

提取治疗CAG复方228个，分析用药规律，药性多温、寒，药味多苦、甘，归经多脾、胃、肝；补气药、理气药、清热药、活血药、补血药为重点功效药物。对比不同功效药物对应靶点与CAG靶点，发现中药治疗CAG的机制与p53信号通路、丝裂原活化蛋白激酶（MAPK）信号通路、磷脂酰肌醇3-激酶/蛋白激酶B（PI3K/Akt）信号通路、叉头转录因子O（FoxO）信号通路、血管内皮生长因子（VEGF）信号通路、低氧诱导因子-1（HIF-1）信号通路等相关，分子对接结果证实中药成分与关键受体蛋白具有对接活性。

结论

研究初步分析了中药治疗CAG的配伍规律，提示补气药、理气药、清热药、活血药、补血药分别从调控细胞增殖与凋亡、调节氧化应激、防御致癌物质、促进胃酸分泌，改善局部微循环等多方面组成了多靶点、多途径、多环节治疗CAG的作用机制网络，为CAG的中药治疗提供了有益探索。

Abstract

Objective

To mine the compatibility rules of patented traditional Chinese medicine (TCM) compound prescriptions for treating chronic atrophic gastritis (CAG) by systems pharmacology and molecular docking methods

and predict the targets and molecular mechanisms of Chinese medicinals with different efficacy in the treatment of CAG.

Method

The TCM compound prescriptions for treating CAG were extracted from the patent system of the China National Intellectual Property Administration. The active components and targets of the prescriptions were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP)

Traditional Chinese Medicine Integrative Database (TCMID)

and UniProt. The candidate targets and pathways of CAG were obtained from GeneCards

DisGeNet

Online Mendelian Inheritance in Man (OMIM)

MalaCards

Kyoto Encyclopedia of Genes and Genomes (KEGG) and Reactome. The gene ontology (GO) functional annotation and KEGG pathway enrichment were realized by R Studio 4.1.2. STRING11.0 was employed to build the protein-protein interaction (PPI) network

and AutoDock Vina 4.2.6 was used for the docking between key targets and components.

Result

A total of 228 TCM compound prescriptions for treating CAG were extracted. The medicinals used in these prescriptions mainly had warm or cold nature

bitter or sweet taste

tropism to the spleen

stomach

and liver meridians

and the efficacy of tonifying Qi

regulating Qi movement

clearing heat

and activating and toniying blood. The prescriptions mainly treated CAG via p53

mitogen-activated protein kinase (MAPK)

phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)

forkhead box protein O (FoxO)

vascular endothelial growth factor (VEGF)

and hypoxia-inducible factor-1 (HIF-1) signaling pathways. Molecular docking results confirmed that the active components in the prescriptions had docking activities with key receptor proteins.

Conclusion

This study preliminarily analyzed the compatibility rules of TCM compound prescriptions in the treatment of CAG. The medicinals with different efficacy treat CAG by regulating cell proliferation

apoptosis

and oxidative stress response

preventing carcinogen production

promoting gastric acid secretion

and improving local microcirculation in a multi-target

multi-pathway

multi-link manner. The findings facilitate the research on the TCM treatment of CAG.

关键词

慢性萎缩性胃炎中药专利复方系统药理学分子对接作用机制

Keywords

chronic atrophic gastritispatented traditional Chinese medicine compound prescriptionsystems pharmacologymolecular dockingmechanism of function

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