Objective

2

Based on serum pharmacochemistry and ultra performance liquid chromatography-quadrupole-time-of-flight mass spectrometry（UPLC-Q-TOF-MS） the transitional components in the serum of rats after intragastric administration of water extract of Alismatis Rhizoma（AR）and salt-processed Alismatis Rhizoma（SAR） were compared.

Method

2

SD rats were randomly divided into blank group

AR group（10 g·kg

-1

） and SAR group（10 g·kg

-1

）

3 rats in each group

the administration groups were given AR and SAR aqueous extracts by gavage

respectively

and the blank group was given an equal volume of drinking water by gavage once in the morning and once in the evening

for 3 consecutive days. Sixty minutes after the last administration

blood was collected from the eye orbits

and the serum samples were prepared. The serum samples were prepared on an ACQUITY UPLC BEH C18 column（2.1 mm×50 mm

1.7 μm） with the mobile phase of acetonitrile（A）-0.1% formic acid aqueous solution（B） in a gradient elution（0-10 min

10%-50% A; 10-27 min

50%-95%A; 27-27.1 min

95%-10% A; 27.1-30 min

10%A）

the data were collected at a flow rate of 0.3 mL·min

-1

in positive ion mode with a scanning range of

m

/

z

100-1 200. Based on the self-constructed chemical composition library of AR

the total ion flow diagrams and secondary MS fragmentation information of the aqueous extracts of AR and SAR

as well as the administered serum and the blank serum

were compared with each other by UNIFI 1.9.2

so as to deduce the possible blood-migrating constituents and their cleavage patterns in the aqueous extracts

and the response intensity ratios of each chemical component were calculated before and after processing.

Result

2

A total of 20 components

including 5 prototypical components and 15 metabolites

were analyzed and deduced from the serum of rats given aqueous extract of AR. And 14 components

including 5 prototypical components and 9 metabolites

were analyzed and deduced from the serum of rats given aqueous extract of SAR. Of these

13 components were common to both of them

including 5 prototypical components and 8 metabolites. The 5 prototypical components were 16-oxoalisol A

alisol A 24-acetate

alisol A

alisol B and alisol C. The metabolites were mainly involved in phase Ⅰ metabolism（oxidation） and phase Ⅱ metabolism（glucuronidation）. There was a big change in the intensity of response of the common components before and after salt-processing

and the response intensities of the prototypical components

16-oxoalisol A

alisol B and alisol C

were elevated

while the type and response intensity of metabolites were generally decreased

and it was hypothesized that the metabolic rate of terpenoids might be slowed down after salt-processing of AR

so that the blood-migrating constituents could participate in the metabolism of the body more in the form of prototypes.

Conclusion

2

Salt-processing of AR may promote the absorption of prototypical components into the blood by slowing down the metabolic rate of terpenoids

which can provide support for the research on material basis of AR and SAR.