Exploring of Transformation Rule of Saponins in Polygalae Radix Before and After Processing Based on Simulated Processing Technology

HE Mengjiao; ZHUANG Ziming; XING Yue; LI Haoran; ZHANG Xuelan; ZHAO Xin

doi:10.13422/j.cnki.syfjx.20230867

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Exploring of Transformation Rule of Saponins in Polygalae Radix Before and After Processing Based on Simulated Processing Technology

更新时间：2023-11-20

- Exploring of Transformation Rule of Saponins in Polygalae Radix Before and After Processing Based on Simulated Processing Technology
  增强出版
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 24, Pages: 169-176(2023)
- 作者机构：
  
  山东中医药大学，济南 250355
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20230867
  CLC： R22;R943.1;R28;O657
- Published：20 December 2023，
  
  Published Online：28 April 2023，
  
  Received：17 March 2023，
- 稿件说明：
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何梦娇,庄梓鸣,邢玥等.基于模拟炮制技术探究远志炮制前后皂苷类成分转化规律[J].中国实验方剂学杂志,2023,29(24):169-176.

HE Mengjiao,ZHUANG Ziming,XING Yue,et al.Exploring of Transformation Rule of Saponins in Polygalae Radix Before and After Processing Based on Simulated Processing Technology[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(24):169-176.
何梦娇,庄梓鸣,邢玥等.基于模拟炮制技术探究远志炮制前后皂苷类成分转化规律[J].中国实验方剂学杂志,2023,29(24):169-176. DOI： 10.13422/j.cnki.syfjx.20230867.

HE Mengjiao,ZHUANG Ziming,XING Yue,et al.Exploring of Transformation Rule of Saponins in Polygalae Radix Before and After Processing Based on Simulated Processing Technology[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(24):169-176. DOI： 10.13422/j.cnki.syfjx.20230867.

摘要

目的

探讨远志甘草汁煮和水煮前后皂苷类成分转化机制和含量变化规律。

方法

采用模拟炮制技术制备远志皂苷B、远志皂苷Z、远志皂苷F、瓜子金皂苷ⅩⅩⅧ单体模拟甘草汁煮品和模拟水煮品，采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法（UPLC-Q-Exactive Orbitrap/MS）分析远志皂苷B、远志皂苷Z、远志皂苷F、瓜子金皂苷ⅩⅩⅧ单体模拟甘草汁煮品和模拟水煮品中的化学成分，采用超高效液相色谱-三重四级杆串联质谱法（UPLC-QQQ-MS/MS）测定远志、甘草汁煮远志、水煮远志中远志皂苷B、远志皂苷Z、远志皂苷F、瓜子金皂苷ⅩⅩⅧ、细叶远志皂苷的含量。

结果

在甘草汁煮和水煮过程中，远志皂苷B可水解生成对甲氧基肉桂酸、desacylsenegin Ⅲ、瓜子金皂苷ⅩⅩⅧ、细叶远志皂苷；远志皂苷Z可水解生成3，4，5-三甲氧基肉桂酸、远志皂苷TF、瓜子金皂苷ⅩⅩⅧ、细叶远志皂苷；远志皂苷F可水解生成3，4，5-三甲氧基肉桂酸、远志皂苷G、瓜子金皂苷ⅩⅩⅧ、细叶远志皂苷；瓜子金皂苷ⅩⅩⅧ可水解生成细叶远志皂苷。远志经甘草汁煮和水煮后远志皂苷B含量明显降低（

0.05，

0.01），而远志皂苷Z、远志皂苷F、瓜子金皂苷ⅩⅩⅧ、细叶远志皂苷含量明显升高（

0.05，

0.01）；甘草汁煮品中远志皂苷Z和细叶远志皂苷含量较水煮品明显升高（

0.05，

0.01），以细叶远志皂苷含量变化最为显著，而远志皂苷B、远志皂苷F、瓜子金皂苷ⅩⅩⅧ在水煮品和甘草汁煮品中的含量无统计学差异。

结论

甘草汁煮和水煮均可使远志中远志皂苷B、远志皂苷Z、远志皂苷F、瓜子金皂苷ⅩⅩⅧ发生水解反应，通过脱糖生成次级苷和苷元（有机酸），从而导致远志炮制后皂苷类成分含量发生明显变化，且甘草汁可促进远志中某些皂苷水解为远志皂苷Z和细叶远志皂苷。本研究为揭示远志炮制机制提供了实验依据。

Abstract

Objective

To investigate the transformation mechanism and content variation of saponins from Polygalae Radix before and after being boiled with licorice juice and water.

Method

Simulated licorice juice boiled products and simulated water boiled products of onjisaponin B， onjisaponin Z， onjisaponin F， polygalasaponin ⅩⅩⅧ were prepared by simulated processing technology， and analyzed by ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry（UPLC-Q-Exactive Orbitrap/MS）. Then the contents of onjisaponin B， onjisaponin Z， onjisaponin F， polygalasaponin ⅩⅩⅧ and tenuifolin in Polygalae Radix， licorice-boiled Polygalae Radix and water-boiled Polygalae Radix were determined by UPLC-triple quadrupole tandem mass spectrometry（UPLC-QQQ-MS/MS）.

Result

During the boiling process with licorice juice and water， onjisaponin B could be hydrolyzed to produce 4-methoxycinnamic acid， desacylsenegin Ⅲ， polygalasaponin ⅩⅩⅧ and tenuifolin， onjisaponin Z could be hydrolyzed to produce 3，4，5-trimethoxycinnamic acid， onjisaponin TF， polygalasaponin ⅩⅩⅧ and tenuifolin， onjisaponin F could be hydrolyzed to produce 3，4，5-trimethoxycinnamic acid， onjisaponin G， polygalasaponin ⅩⅩⅧ and tenuifolin， and polygalasaponin ⅩⅩⅧ was hydrolyzed to produce tenuifolin. After being boiled with licorice juice or water， the content of onjisaponin B decreased significantly（

0.05，

0.01）， but the contents of onjisaponin Z， onjisaponin F， polygalasaponin ⅩⅩⅧ and tenuifolin increased significantly（

0.05，

0.01） in Polygalae Radix. Compared with the water-boiled products， the contents of onjisaponin Z and tenuifolin increased significantly（

0.05，

0.01）， and the change of tenuifolin content was the most significant in the licorice-boiled products.However， there was no significant difference in the content of onjisaponin B， onjisaponin F and polygalasaponin ⅩⅩⅧ between the water-boiled products and the licorice-boiled products.

Conclusion

Being boiled with licorice juice or water can hydrolyze onjisaponin B， onjisaponin Z， onjisaponin F and polygalasaponin ⅩⅩⅧ， and generate secondary glycosides and aglycones（organic acids） through deglycosylation， which leads to obvious changes in the contents of onjisaponins after Polygalae Radix being processed.It is inferred that licorice juice can promote the hydrolysis of some onjisaponins in Polygalae Radix to onjisaponin Z and tenuifolin.This study provides an experimental basis for revealing processing mechanism of Polygalae Radix.

关键词

远志皂苷类成分模拟炮制超高效液相色谱-四极杆-静电场轨道阱高分辨质谱法（UPLC-Q-Exactive Orbitrap/MS）水解反应甘草汁煮减毒增效

Keywords

Polygalae Radixsaponinssimulated processingultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry（UPLC-Q-Exactive Orbitrap/MS）hydrolysis reactionbioling with Glycyrrhizae Radix et Rhizoma juicereduce toxicity and increase effectiveness

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