Study on Changes of Microorganisms and Volatile Components in Aquilariae Lignum Resinatum with Different Aging Years

ZHANG Linfan; GAO Jiaqi; WANG Anjun; LI Tianxiao; LIU Juan

doi:10.13422/j.cnki.syfjx.20231113

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Study on Changes of Microorganisms and Volatile Components in Aquilariae Lignum Resinatum with Different Aging Years

更新时间：2023-10-13

- Study on Changes of Microorganisms and Volatile Components in Aquilariae Lignum Resinatum with Different Aging Years
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 21, Pages: 155-164(2023)
- 作者机构：
  
  1.中国中医科学院中药资源中心，道地药材品质保障与资源持续利用全国重点实验室，北京 100700
  2.天津中医药大学中医药研究院，天津 301617
  3.江苏大学药学院，江苏镇江 212013
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20231113
  CLC： R284.2;R285;R289;R287;R22;R2-031;R33;R24
- Published：05 November 2023，
  
  Published Online：07 September 2023，
  
  Received：16 May 2023，
- 稿件说明：
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张林凡,高佳琪,王安君等.不同陈化年份沉香中微生物及挥发性成分的变化[J].中国实验方剂学杂志,2023,29(21):155-164.

ZHANG Linfan,GAO Jiaqi,WANG Anjun,et al.Study on Changes of Microorganisms and Volatile Components in Aquilariae Lignum Resinatum with Different Aging Years[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(21):155-164.
张林凡,高佳琪,王安君等.不同陈化年份沉香中微生物及挥发性成分的变化[J].中国实验方剂学杂志,2023,29(21):155-164. DOI： 10.13422/j.cnki.syfjx.20231113.

ZHANG Linfan,GAO Jiaqi,WANG Anjun,et al.Study on Changes of Microorganisms and Volatile Components in Aquilariae Lignum Resinatum with Different Aging Years[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(21):155-164. DOI： 10.13422/j.cnki.syfjx.20231113.

摘要

目的

研究旨在探讨沉香陈化过程中，真菌群落多样性和挥发性成分的变化规律，探索二者的内在联系。

方法

收集不同陈化时间的沉香样品，应用高通量测序技术，分析真菌物种注释结果及丰度信息，并通过

及

多样性分析方法，对真菌群落结构组成、动态变化进行分析；同时提取不同陈化时间沉香的挥发油，并通过二维气相色谱-高分辨飞行时间质谱技术进行分离和鉴定。

结果

从5组沉香样品的挥发性成分中共检测到61个化合物，分别为2种单萜、24种倍半萜、1种二萜、13种芳香烃、9种烷烃和12种其他化合物；其中陈化1年沉香中分离出的挥发性化合物种类最多，陈化2年沉香的挥发性成分在总成分中所占比例最大。ITS扩增子测序分析发现5组沉香样品中的真菌隶属于162属，优势真菌属为

Kirschsteiniothelia

、曲霉属

Aspergillus

、二孢属

Lasiodiplodia

、

Phaeoacremonium

、木霉菌属

Trichoderma

等；多样性指数显示，陈化4年沉香的真菌多样性明显高于新品和陈化1~3年的沉香。

结论

在陈化过程中，沉香挥发性成分的含量和组成均发生明显变化；随着陈化年份增加，沉香中的真菌多样性增加，芳香烃相对含量显著降低，而倍半萜相对含量显著增加。总体而言，陈化过程有利于沉香倍半萜类物质的转化及真菌群落的丰富。

Abstract

Objective

This study aims to investigate the changes in fungal community diversity and volatile components during the aging process of Aquilariae Lignum Resinatum and explore the internal relationship between them.

Method

Aquilariae Lignum Resinatum samples with different aging years were collected. High-throughput sequencing was employed to analyze the fungal diversity and abundance， and

and

diversity indicators were calculated to reveal the composition and dynamic changes of the fungal community. In addition， the essential oils of Aquilariae Lignum Resinatum with different aging years were extracted， separated， and identified by two-dimensional gas chromatography-high resolution time-of-flight mass spectrometry.

Result

A total of 61 compounds were identified from the volatile components of five groups of Aquilariae Lignum Resinatum samples， including 2 monoterpenes， 24 sesquiterpenoids， 1 diterpene， 13 aromatic hydrocarbons， 9 alkanes， and 12 other compounds. Among them， the volatile compounds isolated from the sample aged for 1 year had the largest number， and those from the sample aged for 2 years accounted for the largest proportion of the total components. The internal transcribed spacer（ITS） amplicon sequencing revealed that the fungi in the five groups of samples belonged to 162 genera.

Kirschsteiniothelia

，

Aspergillus

，

Lasiodiplodia

，

Phaeoacremonium

， and

Trichoderma

were the dominant fungal genera. The fungal diversity in the Aquilariae Lignum Resinatum sample aged for 4 years was significantly higher than that in the samples aged for 0 to 3 years.

Conclusion

The volatile component content and composition of Aquilariae Lignum Resinatum altered dramatically during aging. The aging of Aquilariae Lignum Resinatum was accompanied by the increasing fungal diversity， decreasing relative content of aromatic hydrocarbons， and increasing relative content of sesquiterpenoids. In general， aging was beneficial to the transformation of sesquiterpenoids and the enrichment of fungi.

关键词

白木香沉香陈化微生物多样性挥发性成分

Keywords

Aquilaria sinensisAquilariae Lignum Resinatumagingmicrobial diversityvolatile components

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