Chemical Components Distribution and Transcriptome Analysis of Different Tissues from <italic style="font-style: italic">Codonopsis pilosula</italic>

JI Jiaojiao; DAI Junli; LI Jiankuan; CAO Lingya; XIONG Xin; GAO Jianping

doi:10.13422/j.cnki.syfjx.20231261

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Chemical Components Distribution and Transcriptome Analysis of Different Tissues from Codonopsis pilosula

更新时间：2023-08-22

- Chemical Components Distribution and Transcriptome Analysis of Different Tissues from Codonopsis pilosula
  增强出版
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 29, Issue 18, Pages: 117-125(2023)
- 作者机构：
  
  山西医科大学药学院，太原 030000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20231261
  CLC： R22;R28;Q786;O657
- Published：20 September 2023，
  
  Published Online：06 July 2023，
  
  Received：04 May 2023，
- 稿件说明：
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吉姣姣,戴俊利,李建宽等.党参不同组织化学成分分布及转录组学分析[J].中国实验方剂学杂志,2023,29(18):117-125.

JI Jiaojiao,DAI Junli,LI Jiankuan,et al.Chemical Components Distribution and Transcriptome Analysis of Different Tissues from Codonopsis pilosula[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(18):117-125.
吉姣姣,戴俊利,李建宽等.党参不同组织化学成分分布及转录组学分析[J].中国实验方剂学杂志,2023,29(18):117-125. DOI： 10.13422/j.cnki.syfjx.20231261.

JI Jiaojiao,DAI Junli,LI Jiankuan,et al.Chemical Components Distribution and Transcriptome Analysis of Different Tissues from Codonopsis pilosula[J].Chinese Journal of Experimental Traditional Medical Formulae,2023,29(18):117-125. DOI： 10.13422/j.cnki.syfjx.20231261.

摘要

目的

分析党参不同组织转录组特征，解析党参根中活性成分积累的遗传基础，为党参的高品质生产与种植提供理论依据。

方法

选取盛花期党参根、茎、叶、花不同组织为实验材料，采用高效液相色谱法（HPLC）检测党参苷Ⅰ、党参炔苷、苍术内酯Ⅲ含量，利用RNA-Seq对不同组织进行转录组测序，通过基因本体（GO）、京都基因和基因组百科全书（KEGG）数据库富集分析筛选分析差异表达基因，探讨党参不同组织化学成分分布特征及转录谱特征。

结果

党参根中党参多糖及党参苷Ⅰ含量显著高于其他各组织。党参根转录谱与茎、叶、花存在显著差异，差异基因表达聚类分析、GO、KEGG富集分析发现差异基因主要富集在黄酮类、苯丙烷类生物合成、蔗糖淀粉代谢、植物激素信号传导、植物病原菌互作、MAPK级联信号传递、三磷酸腺苷结合转运盒（ABC）转运蛋白等途径。苯丙烷类化合物生物合成相关基因在根、花中表达量显著上调，ABC转运蛋白则多在花中高表达，党参多糖合成关键酶基因蔗糖：蔗糖1-果糖基转移酶（1-

SST

）、果聚糖1-外切水解酶（1-

Feh

）及大量与次生代谢产物积累密切相关的胁迫响应基因等在党参根中显著上调表达。

结论

党参根中活性成分含量与转录谱特征与茎、叶、花等组织存在显著差异，表现出组织特异性，同时胁迫响应相关基因及活性成分菊粉型果聚糖、苯丙烷类化合物合成相关基因在根中表达上调。

Abstract

Objective

The transcriptome characteristics of different tissues of

Codonopsis pilosula

were analyzed to illustrate the genetic basis of the accumulation of active ingredients in the root of

C. pilosula

， and to provide theoretical basis for its high-quality production and cultivation.

Method

Different tissues of

C. pilosula

at flowering stage were selected as experimental materials， and the contents of tangshenoside Ⅰ， lobetyolin and atractylenolide Ⅲ were detected by high performance liquid chromatography（HPLC）. RNA-Seq was used to perform transcriptome sequencing of different tissues， and the differentially expressed genes were screened and analyzed by Gene Ontology（GO） and Kyoto Gene and Encyclopedia of Genes and Genomes（KEGG） enrichment analysis， in order to explore the characteristics of active compound distribution and the transcriptional profiles.

Result

The contents of polysaccharides and tangshenoside Ⅰ in the root of

C. pilosula

were significantly higher than those in other tissues. The transcriptional profiles of the root were significantly different from those of stem， leaf and flower. Cluster analysis， GO and KEGG enrichment analysis of differential gene expression showed that the differential expression genes were mainly enriched in flavonoid and phenylpropanoid biosynthesis， sucrose-starch metabolism， plant hormone signal transduction， plant-pathogen interaction， mitogen-activated protein kinase（MAPK） cascade signal transduction， Adenosine triphosphate（ATP）-binding cassette（ABC） transporter and other pathways. The expression of genes related to biosynthesis of phenylpropanoid compounds were significantly up-regulated in the roots and flowers， and ABC transporter proteins were mostly highly expressed in the flowers. The expression of key enzyme genes for polysaccharide synthesis， such as sucrose：sucrose 1-fructosyltransferase（1-

SST

） and fructan 1-exohydrolase（1-

Feh

）， were significantly up-regulated in the roots， and a large number of stress-responsive genes closely related to the accumulation of secondary metabolites were significantly up-regulated in the roots.

Conclusion

The active compound content and transcriptional profiles in

C. pilosula

roots were significantly different from those in stem， leaf， flower and other tissues， showing tissue specificity. Meanwhile， the genes related to stress response and biosynthesis of active compound， such as fructan and phenylpropanoid compounds， were up-regulated in roots of

C. pilosula

关键词

党参组织分布转录组学活性成分基因代谢途径

Keywords

Codonopsis pilosulatissue distributiontranscriptomicsactive componentsgenesmetabolic pathways

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Chemical Components Distribution and Transcriptome Analysis of Different Tissues from Codonopsis pilosula

DOI：10.13422/j.cnki.syfjx.20231261

摘要

Abstract

关键词

Keywords

references