“A significant progress has been made in the research on detoxification, meridian dredging, and liver regulating formula to protect pancreatic beta cells. This study used a db/db mouse model to investigate how the traditional Chinese medicine formula targets NOD like receptor protein 3 (NLRP3) inflammasome through the bile acid G protein coupled receptor 5 (TGR5)/cyclic adenosine monophosphate (cAMP) signaling pathway, thereby exerting a protective effect on pancreatic beta cells. The research results show that Jiedu Tongluo Tiaogan Formula can significantly reduce fasting blood glucose levels, improve oral glucose tolerance, and lower fasting insulin levels in db/db mice. In addition, this prescription can also significantly reduce the levels of interleukin-6 (IL-6), tumor necrosis factor - α (TNF - α) and IL-1 β in mice, which are important indicators of diabetes related inflammation. In terms of mechanism, Jiedu Tongluo Tiaogan Formula can promote the upregulation of TGR5 and cAMP mRNA, and downregulate the mRNA expression of NLRP3, apoptosis related spotted protein (ASC), and caspase-1. At the same time, the formula can also increase TGR5 protein expression, reduce NLRP3, Caspase-1, and ASC protein expression, and increase cAMP content. These results indicate that Jiedu Tongluo Tiaogan Formula protects pancreatic beta cells by regulating the TGR5/cAMP signaling pathway, inhibiting the activation of NLRP3 inflammasome. This study provides new ideas and methods for the treatment of diabetes, and also provides a scientific basis for the application of traditional Chinese medicine prescriptions in the treatment of diabetes. In the future, we can further investigate the specific mechanism of action of Jiedu Tongluo Tiaogan Formula, in order to provide more effective drug choices for the treatment and prevention of diabetes.”