Mechanism of Herbal Cake-separated Moxibustion in Improving Neuroimmune Inflammation in Rats with Chronic Fatigue Syndrome by Interfering TLR4/MyD88/NF-<italic style="font-style: italic">κ</italic>B Pathway

ZHAI Chuntao; HOU Yawei; SHI Linjuan; WANG Yixiao; LI Wei; TIAN Yuefeng

doi:10.13422/j.cnki.syfjx.20241140

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Mechanism of Herbal Cake-separated Moxibustion in Improving Neuroimmune Inflammation in Rats with Chronic Fatigue Syndrome by Interfering TLR4/MyD88/NF-κB Pathway

更新时间：2025-01-16

- Mechanism of Herbal Cake-separated Moxibustion in Improving Neuroimmune Inflammation in Rats with Chronic Fatigue Syndrome by Interfering TLR4/MyD88/NF-κB Pathway
  增强出版
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 31, Issue 4, Pages: 140-149(2025)
- 作者机构：
  
  山西中医药大学第二临床学院，山西晋中 030619
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20241140
  CLC： R277;R285;R746
- Published：20 February 2025，
  
  Published Online：17 July 2024，
  
  Received：03 June 2024，
- 稿件说明：
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ZHAI CHUNTAO, HOU YAWEI, SHI LINJUAN, et al. Mechanism of Herbal Cake-separated Moxibustion in Improving Neuroimmune Inflammation in Rats with Chronic Fatigue Syndrome by Interfering TLR4/MyD88/NF-κB Pathway. [J]. Chinese journal of experimental traditional medical formulae, 2025, 31(4): 140-149.
DOI：

ZHAI CHUNTAO, HOU YAWEI, SHI LINJUAN, et al. Mechanism of Herbal Cake-separated Moxibustion in Improving Neuroimmune Inflammation in Rats with Chronic Fatigue Syndrome by Interfering TLR4/MyD88/NF-κB Pathway. [J]. Chinese journal of experimental traditional medical formulae, 2025, 31(4): 140-149. DOI： 10.13422/j.cnki.syfjx.20241140.

摘要

目的

基于Toll样受体4/髓样分化因子88/核转录因子-

B（TLR4/MyD88/NF-

B）信号通路探讨经方逍遥散为药饼进行施灸改善慢性疲劳综合征（CFS）大鼠神经免疫炎症反应的作用机制。

方法

50只6~8周龄SPF级SD大鼠随机分为正常组、模型组、假药饼灸组、中药灌胃组、隔药饼灸组，每组10只。除正常组外其余各组进行持续21 d的造模，造模后进行行为学检测，隔药饼灸组与假药饼灸组对“神阙”“关元”“足三里”“期门”穴进行干预，中药灌胃组给予逍遥散悬浊液灌胃干预，连续干预10 d后进行行为学检测，酶联免疫吸附测定法（ELISA）检测各组大鼠血清白细胞介素-1

（IL-1

）、白细胞介素-6（IL-6）、肿瘤坏死因子-

（TNF-

）含量及海马组织中IL-1

、IL-6、TNF-

、NF-

B含量，苏木素-伊红（HE）染色观察大鼠海马组织的形态变化，实时荧光定量聚合酶链式反应（Real-time PCR）检测大鼠海马组织中TLR4、MyD88、NF-

B mRNA水平，蛋白免疫印迹法（Wester

n blot）检测大鼠海马组织中TLR4、MyD88、NF-

B p65蛋白表达。

结果

与正常组比较，模型组旷场实验直立次数显著减少（

0.01），运动总距离、静止时间及中心区域持续时间显著缩短（

0.01）；悬尾实验不动时间显著增加（

0.01），挣扎次数显著减少（

0.01）；血清、海马组织炎性因子IL-1

、IL-6、TNF-

水平，海马组织NF-

B水平及TLR4、MyD88、NF-

B mRNA表达水平显著升高（

0.01）。经治疗后，与模型组比较，各治疗组旷场实验运动总路程及直立次数显著增加（

0.01），静止时间及中心持续时间明显延长（

0.05，

0.01）；悬尾实验不动时间显著缩短（

0.01），挣扎次数明显增加（

0.05）；血清、海马组织IL-1

、IL-6、TNF-

水平，海马组织NF-

B水平及TLR4、NF-

B mRNA表达水平明显降低（

0.05，

0.01）。与假药饼灸组比较，隔药饼灸组旷场实验中心区域持续时间明显延长（

0.05），直立次数显著增加（

0.01）；悬尾实验不动时间缩短（

0.01），挣扎次数显著增加（

0.01）；中药灌胃组血清TNF-

水平显著降低（

0.01），中药灌胃组及隔药饼灸组血清、海马组织IL-6水平、海马组织IL-1

、TNF-

、NF-

B水平及TLR4、MyD88、NF-

B mRNA表达水平明显降低（

0.05，

0.01）。与中药灌胃组比较，隔药饼灸组旷场实验直立次数显著增加（

0.01）；悬尾实验不动时间缩短（

0.01），挣扎次数增加（

0.01）；血清IL-1

、海马组织TNF-

水平及TLR4、MyD88、NF-

B mRNA表达水平明显降低（

0.05，

0.01）。

结论

隔药饼灸能够有效改善CFS大鼠的疲劳状态与记忆功能，调控机体神经免疫炎性反应，其机制可能与调节TLR4/MyD88/NF-

B信号通路有关。

Abstract

Objective

To explore the mechanism of herbal cake-separated moxibustion using the classical formula Xiaoyaosan in alleviating neuroimmune inflammatory responses in chronic fatigue syndrome （CFS） rats， based on the regulation of the toll-like receptor 4 （TLR4）/myel

oid differentiation factor 88 （MyD88）/nuclear transcription factor-

B （NF-

B） signaling pathway.

Methods

Fifty SPF-grade SD rats aged 6-8 weeks were randomly divided into five groups： Normal group， model group， sham herbal cake moxibustion group， Chinese medicine intragastric administration group， and herbal cake-separated moxibustion group， with 10 rats in each group. Except for the normal group， all other groups underwent a 21-day modeling process， followed by behavioral testing. The herbal cake-separated moxibustion and sham herbal cake moxibustion groups received interventions at the Shenque （CV8）， Guanyuan （CV4）， Zusanli （ST36）， and Qimen （LR14） acupoints. The Chinese medicine intragastric administration group was treated with a Xiaoyaosan suspension via gavage. Behavioral tests were conducted after 10 days of continuous intervention. Serum levels of interleukin （IL）-1

， IL-6， and tumor necrosis factor-

（TNF-

）， as well as hippocampal levels of IL-1

， IL-6， TNF-

， and NF-

B， were detected by enzyme-linked immunosorbent assay （ELISA）. Morphological changes in the hippocampus were observed using hematoxylin-eosin （HE） staining. Real-time quantitative polymerase chain reaction （Real-time PCR） was used to detect mRNA expression levels of TLR4， MyD88， and NF-

B in the hippocampus. Western blot analysis was performed to detect the relative expression levels of TLR4， MyD88， NF-

B， and p65 proteins in the hippocampus.

Results

Compared with the normal group， the model group showed a significant decrease in upright times during the open field test （

0.01）， as well as significant reductions in total movement distance， resting time， and center region duration （

0.01）. In the tail suspension test， immobility time increased （

0.01）

， and struggle times decreased （

0.01）. Serum and hippocampal levels of IL-1

， IL-6， and TNF-

， as well as hippocampal NF-

B levels and TLR4， MyD88， and NF-

B mRNA expression， were significantly elevated （

0.01）. After treatment， compared with the model group， the total movement distance and upright times in the open field test were significantly increased in all treatment groups （

0.01）， while resting time and center region duration were notably prolonged （

0.05，

0.01）. Immobility time in the tail suspension test was significantly shortened （

0.01）， and struggle times significantly increased （

0.05）. Serum and hippocampal levels of IL-1

， IL-6， TNF-

， hippocampal NF-

B levels， and TLR4 and NF-

B mRNA expression were significantly reduced （

0.05，

0.01）. Compared with the sham herbal cake moxibustion group， the herbal cake-separated moxibustion group showed a significant extension in center region duration during the open field test （

0.05） and a significant increase in upright times （

0.01）. In the tail suspension test， immobility time was reduced （

0.01）， and struggle times increased （

0.01）. Serum TNF-

levels in the Chinese medicine intragastric administration group were significantly reduced （

0.01）， while serum IL-6 levels， as well as hippocampal levels of IL-1

， TNF-

， NF-

B， and TLR4， MyD88， and NF-

B mRNA expression， were significantly decreased in both the Chinese medicine intragastric administration group and the herbal cake-separated moxibustion group （

0.05，

0.01）. Compared with the Chinese medicine intragastric administration group， the herbal cake-separated moxibustion group exhibited significantly increased upright times in the open field test （

0.01）. In the tail suspension test， immobility time was reduced （

0.01）， and struggle times increased （

0.01）. Serum IL-1

， hippocampal TNF-

levels， and TLR4， MyD88， and NF-

B mRNA expression were significantly decreased （

0.05，

0.01）.

Conclusion

Herbal cake-separated moxibustion effectively improves fatigue and memory function in CFS rats， regulates neuroimmune inflammatory responses， and its mechanism may be related to the modulation of the TLR4/MyD88/NF-

B signaling pathway.

关键词

隔药饼灸神经免疫炎症慢性疲劳综合征逍遥散

Keywords

herbal cake-separated moxibustionneuroimmune inflammationchronic fatigue syndrome （CFS）Xiaoyaosan

references

边红恩，陈团营，单海军.重灸疗法对心脾两虚型慢性疲劳综合征患者临床疗效的研究［J］.中医药信息，2021，38（3）：65-68.

BIAN H E，CHEN T Y，SHAN H J.Clinical effect of heavy moxibustion in treating chronic fatigue syndrome （deficiency of heart and spleen）［J］.Inform Tradit Chin Med，2021，38（3）：65-68.

徐小珊，马伟，熊罗节，等. 隔药饼灸对慢性疲劳综合征大鼠血乳酸及AMPK/PGC-1α信号通路的影响［J］.针刺研究，2022，47（10）： 878-884.

XU X S，MA W，XIONG L J，et al.Effect of herbal cake-separated moxibustion on behavioral stress reactions and blood lactic acid level and muscular AMPK/PGC-1α signaling in rats with chronic fatigue syndrome［J］.Acupunct Res，2022，47（10）： 878-884.

梁蔚莉，何采辉，易展.背俞穴隔药饼灸治疗慢性疲劳综合征疗效观察［J］.上海针灸杂志，2018，37（8）：843-846.

LIANG W L，HE C H，YI Z.Therapeutic observation of herb-partitioned moxibustion at back-shu points for chronic fatigue syndrome［J］.Shanghai J Acup Moxib，2018，37（8）：843-846.

徐秋露，韩秀珍，李悦，等.逍遥散及其类方治疗慢性疲劳综合征研究进展［J］.江苏中医药，2023，55（5）：74-77.

XU Q L，HAN X Z，LI Y，et al.Review of treatment of chronic fatigue syndrome with Xiaoyao powder and lts associated prescriptions［J］.Jiangsu J Tradit Chin Med，2023，55（5）：74-77.

史娇.逍遥散加味治疗肝郁脾虚型慢性疲劳综合征的临床疗效分析［J］.系统医学，2019，4（1）：135-137.

SHI J.Analysis of clinical curative effect of modified Xiaoyao San in treatment of chronic fatigue syndrome of liver depression and spleen deficiency［J］.Syst Med，2019，4（1）：135-137.

王若禹.逍遥散方隔药饼灸治疗肝郁脾虚型慢性疲劳综合征的临床观察［D］.太原：山西中医药大学，2023：26.

WANG R Y.Clinical observation on the treatment of chronic fatigue syndrome of liver depression and spleen deficiency type with remote scattering formula-separated medicinal cake moxibustion［D］.Taiyuan：Shanxi University of Chinese Medicine，2023：26

TIAN Y F，FANG H，SUN N N，et al.Effect of different moxibustion interventions on hepatic histomorphology and PD-1 expression in immunosuppressed rabbits［J］.World J Acupunct-Moxibust，2021，31（4）：296-301.

徐小珊，熊罗节，马伟，等.隔药饼灸对慢性疲劳综合征大鼠免疫蛋白及T细胞调节因子Tim-3、LAG-3的影响［J］.中国中医基础医学杂志，2023，29（10）：1666-1671.

XU X S，XIONG L J，MA W，et al.Effect of medicinal cake moxibustion on lmmune proteins and T cel regulatory factors Tim-3 and LAG-3 in rats with chronic fatigue syndrome［J］.J Basic Chin Med，2023，29（10）：1666-1671.

汤家铭，陈民利.医学实验动物学［M］.北京：中国中医药出版社，2012：7.

TANG J M，CHEN M L.Laboratory animal science［M］.Beijing：China Press of Chinese Medicine，2012：7.

杨燕，王德龙，郭静，等. 电针对慢性疲劳综合征大鼠行为学及大鼠脑组织病理形态学的影响［J］.中国医药导报， 2018，15（26）：9-12.

YANG Y，WANG D L，GUO J，et al.Effect of electroacupuncture on behavior and pathomorphology of brain tissue in rats with chronic fatigue syndrome［J］.China Medical Herald，2018，15（26）：9-12.

高怀林，吴以岭，朱慧明，等.慢性疲劳综合征动物模型的建立及对血管内皮功能的影响［J］.中国中医基础医学杂志，2009，15（12）：904-907.

GAO H L，WU Y L，ZHU H M，et al.Stablishment of animal model with chronie fatigue syndrome and its influence on functions of blood vessel endothelium［J］.J Basic Chin Med，2009，15（12）：904-907.

李忠仁. 实验针灸学［M］. 北京：中国中医药出版社，2003：327-329.

LI Z R.Experimental acupuncture science［M］.Beijing：China Press of Chinese Medicine，2003：327-329.

张力，张强，刘凯利，等.中医辨证分型治疗慢性疲劳综合征的临床研究进展［J］.现代中西医结合杂志，2022，31（18）：2623-2628.

ZHANG L，ZHANG Q，LIU K L，et al.Clinical research progress on the treatment of chronic fatigue syndrome based on syndrome differentiation in traditional Chinese medicine［J］.Mod J Integr Tradit Chin West Med，2022，31（18）：2623-2628.

董东梅，常诚.健忘中医论治探讨［J］.辽宁中医杂志，2017，44（5）：945-946.

DONG D M，CHANG C.TCM treatment for forgetfulness［J］.Liaoning J Tradit Chin Med，2017，44（5）：945-946.

翟春涛，田岳凤，金晓婵.中药透皮吸收及促透作用的特色分析［J］.山西中医药大学学报，2017，18（6）：67-69.

ZHAI C T，TIAN Y F，JIN X C.Analysis of the characteristics of transdermal absorption and permeation promotion of traditional Chinese medicine［J］.J Shanxi Univ Chin Med，2017，18（6）：67-69.

刘长征，雷波.艾灸神阙穴对慢性疲劳综合征大鼠运动能力的影响［J］.右江医学，2022，50（9）：653-656.

LIU C Z，LEI B.Effect of moxibustion at Shenque point on exercise capacity of rats with chronic fatigue syndrome［J］.Chin Youjiang Med J，2022，50（9）：653-656.

林玉敏，江钢辉.艾灸气海、关元穴治疗慢性疲劳综合征的临床疗效及其对大鼠免疫功能的影响［J］.广西医学，2017，39（10）：1546-1549.

LIN Y M，JIANG G H.Clinical efficacy of moxibustion at Qihai and Guanyuan acupoints for treatment of chronic fatigue syndromes and its influence on immune function of rats［J］.Guangxi Med J，2017，39（10）：1546-1549.

冯楚文，屈媛媛，范桢亮，等.基于集合可视化分析系统探索灸法治疗慢性疲劳综合征的选穴规律［J］.针灸临床杂志，2021，37（10）：43-51.

FENG C W，QU Y Y，FAN Z L，et al.Acupoints regularity of moxibustion treating CFS based on collection visualization analysis system［J］.J Clin Acup Moxib，2021，37（10）：43-51.

PATARCA R，ANN N Y.Cytokines and chronic fatigue syndrome［J］.Acad Sci，2001，933（1）：185-200.

GUO Q Y，GENG Y N，HAN X，et al.Amelioration of cognitive dysfunction in diabetic rats by Jianpi Qinghua prescription［J］.Chin J Exp Tradit Med Form，2023，29（8）：195-202.

郭秋月，耿艳娜，韩煦，等.健脾清化方对糖尿病大鼠认知功能障碍的改善作用［J］.中国实验方剂学杂志，2023，29（8）：195-202.

NAKATOMI Y，MIZUNO K，ISHII A，et al.Neuroinflammation in patients with chronic fatigue syndrome/myalgic encephalomyelitis：An 11C-（R）-PK11195 PET study［J］.J Nucl Med，2014，55（6）：945-950.

MAES M，TWISK F N，KUBERA M，et al.Evidence for inflammation and activation of cell-mediated immunity in myalgic encephalomyelitis/chronic fatigue syndrome（ME/CFS）：Increased interleukin-1，tumor necrosis factor-4α，PMN-elastase，lysozyme and neopterin［J］.J Affect Disord，2012，136（1）：933-939.

DANTZER R，O′CONNOR J C，FREUND G G，et al.From inflammation to sickness and depression：When the immune system subjugates the brain［J］.Nat Rev Neurosci，2008，9（1）：46-56.

郭锦荣，朱立新，彭垒，等.基于TLR4/MyD88/NF-κB通路研究降脂通脉饮对高脂血症模型大鼠的影响［J］. 河南中医， 2024， 44 （3）： 361-366.

GUO J R，ZHU L X，PENG L，et al.The influence of granules for invigorating circulation and reducing blood lipid on hyperlipidemia model rats based on TLR4/MyD88/NF-κB pathway［J］.Henan Tradit Chin Med，2024， 44 （3）： 361-366.

KAWAI T，AKIRA S.The role of pattern-recognition receptors in innate immunity：Update on Toll-like receptors［J］.Nat Immunol，2010，11（5）：373-384.

RASSA J C，MEYERS J L，ZHANG Y，et al. Murine retroviruses activate B cells via interaction with Toll-like receptor 4［J］.P Natl Acad Sci USA，2002，99（4）：2281-2286.

王双乐，刘欢，倪海滨.血府逐瘀汤对COPD模型小鼠炎症因子及TLR4/MyD88/NF-κB信号通路的影响［J］. 中国中医急症， 2022， 31 （5）： 769-772.

WANG S L，LIU H，NI H B.Effects of Xuefu Zhuyu decoction on inflammatory factors and TLR4/MyD88/NF-κB signaling pathway in COPD model mice［J］.J Emer Tradit Chin Med，2022，31 （5）： 769-772.

PADOVA F D，QUESNIAUX V F J，RYFFEL B.MyD88 as a therapeutic target for inflammatory lung diseases［J］. Expert Opin Ther Tar，2018，22（5）：401-408.

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⁰

Mechanism of Herbal Cake-separated Moxibustion in Improving Neuroimmune Inflammation in Rats with Chronic Fatigue Syndrome by Interfering TLR4/MyD88/NF-κB Pathway

DOI：10.13422/j.cnki.syfjx.20241140

摘要

Abstract

关键词

Keywords

references