WANG Zimo,LIU Bo,WANG Xiaoqing,et al.Bioactive Secondary Metabolites from Talaromyces sp. TP21, an Endophytic Fungus of Stellera chamaejasme[J].Chinese Journal of Experimental Traditional Medical Formulae,2024,30(23):205-213.
WANG Zimo,LIU Bo,WANG Xiaoqing,et al.Bioactive Secondary Metabolites from Talaromyces sp. TP21, an Endophytic Fungus of Stellera chamaejasme[J].Chinese Journal of Experimental Traditional Medical Formulae,2024,30(23):205-213. DOI: 10.13422/j.cnki.syfjx.20241415.
Bioactive Secondary Metabolites from Talaromyces sp. TP21, an Endophytic Fungus of Stellera chamaejasme增强出版
TP21 were isolated by high performance liquid chromatography (HPLC), normal phase and reversed phase column chromatography combined with molecular networking and bioassay-guided fractionation, and their structures were determined by nuclear magnetic resonance (NMR) and high resolution mass spectrometry (HR MS). The inhibitory effects of the compounds on the growth of the lung cancer cell line A549 and the liver cancer cell line Hep G2 were measured by themethyl thiazolyl tetrazolium (MTT) method. The antimicrobial activities of the compounds were measured with
Staphylococcus aureus
and human oral cavity-derived
Saccharomyces cerevisiae
as the indicator microorgani
sms.
Result
2
Seventeen compounds were isolated from the secondary metabolites of
Talaromyces
sp. TP21 and identified as ergochrome C (
1
), daldiniaeschsone A (
2
),
seco
-blennolide B (
3
), penitholabene (
4
), penicichrysogene A (
5
), orsellinic acid (
6
), griseofulvin (
7
), isorhodoptilometrin (
8
), (+)-5-chloromitorubrinic acid (
9
), penicillixanthone A (
10
), pentacecilide B (
11
), pentacecilide C (
12
), chrodrimanin C (
13
), chrodrimanin E (
14
), chrodrimanin H (
15
), chrodrimanin F (
16
), and 3-hydroxypentacecilide A (
17
).
Conclusion
2
Compounds
1
-
5
,
11
-
12
, and
17
were isolated from
Talaromyces
for the first time. Among them, compounds
4
,
7
, and
10
-
12
exhibited inhibitory activities against the growth of A549 and Hep G2 cells, with the median inhibitory concentration below 50 μmol·L
-1
. Furthermore, compounds
9
and
10
showed inhibitory activities against
S. aureus
and human oral cavity-derived
S. cerevisiae
, with the minimum inhibitory concentration of 8-128 mg·L
-1
.
关键词
Keywords
references
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