Effect of Thyme Herbal Tea on Proliferation of Human Coronavirus OC43 <italic style="font-style: italic">in vitro</italic> and <italic style="font-style: italic">in vivo</italic>

TIAN Jixiang; ZHANG Tongtong; CHANG Yuning; XIE Peifang; DONG Shuwei; ZHAO Xiaoang; WANG Yun; ZHAO Chunhui; WU Hongwei; ZHANG Amei; LI Haizhou; XIA Xueshan; ZHANG Huamin

doi:10.13422/j.cnki.syfjx.20241504

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Effect of Thyme Herbal Tea on Proliferation of Human Coronavirus OC43 in vitro and in vivo

更新时间：2025-05-28

- Effect of Thyme Herbal Tea on Proliferation of Human Coronavirus OC43 in vitro and in vivo
  增强出版
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 30, Issue 23, Pages: 81-89(2024)
- 作者机构：
  
  1.中国中医科学院中医基础理论研究所，北京 100700
  2.中国中医科学院中药研究所，道地药材与品质保障全国重点实验室，北京 100700
  3.昆明理工大学生命科学与技术学院，昆明 650500
  4.昆明医科大学云南省公共卫生与生物安全重点实验室，昆明 650500
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20241504
  CLC： R2-0;R22;R285.5;R289;R33
- Received：06 September 2024，
  
  Accepted：23 September 2024，
  
  Online First：29 September 2024，
  
  Published：05 December 2024
- 稿件说明：
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田纪祥,张彤彤,常雨宁等.百里香药茶对人冠状病毒OC43体内外增殖的影响[J].中国实验方剂学杂志,2024,30(23):81-89.

TIAN Jixiang,ZHANG Tongtong,CHANG Yuning,et al.Effect of Thyme Herbal Tea on Proliferation of Human Coronavirus OC43 in vitro and in vivo[J].Chinese Journal of Experimental Traditional Medical Formulae,2024,30(23):81-89.
田纪祥,张彤彤,常雨宁等.百里香药茶对人冠状病毒OC43体内外增殖的影响[J].中国实验方剂学杂志,2024,30(23):81-89. DOI： 10.13422/j.cnki.syfjx.20241504.

TIAN Jixiang,ZHANG Tongtong,CHANG Yuning,et al.Effect of Thyme Herbal Tea on Proliferation of Human Coronavirus OC43 in vitro and in vivo[J].Chinese Journal of Experimental Traditional Medical Formulae,2024,30(23):81-89. DOI： 10.13422/j.cnki.syfjx.20241504.

摘要

目的

探究百里香药茶（BLX）对人冠状病毒OC43（HCoV-OC43）的体内外抗病毒活性及作用机制。

方法

通过超高效液相色谱-质谱（UPLC-MS）分析BLX化学成分，利用HCoV-OC43细胞模型检测BLX的体外毒性及其对该病毒的抑制作用，采用时间差异给药法检测药物作用阶段，实时荧光定量聚合酶链式反应（Real-time PCR）法检测病毒基因拷贝数，利用HCoV-OC43小鼠模型检测BLX的体内毒性及其对小鼠体质量和生存周期变化的影响，通过免疫组化技术检测脑和肺组织中病毒蛋白表达。

结果

从BLX中鉴定出11种化合物；在HCoV-OC43感染的HRT-18细胞中，BLX的半数毒性浓度（CC

）为（13 859.56±319） mg·L

-1

，半数抑制浓度（IC

）为（1 439.09±200） mg·L

-1

，其选择指数范围为8.26~11.44。与病毒组比较，BLX在1 500、1 000、500 mg·L

-1

剂量下均可明显抑制病毒复制（

0.05，

0.01），该药物在病毒复制早期即可发挥抗病毒作用，且对病毒吸附过程的干扰大于对病毒入胞过程的干扰（

0.05）。在HCoV-OC43感染的小鼠模型中，BLX在1 200、600 mg·kg

-1

·d

-1

剂量下可缓解被感染小鼠的症状、延长生存期并降低死亡率，有效抑制脑组织中（

0.01）和肺组织（

0.01）中病毒核衣壳mRNA表达水平；BLX在1 200 mg·kg

-1

剂量下可抑制脑组织（

0.01）和肺组织（

0.01）中病毒核衣壳蛋白的表达。

结论

BLX含有多种具有抗病毒活性的化学成分，可在体内外抑制HCoV-OC43复制，该药物可能通过干扰病毒吸附等方式发挥其抗病毒活性。该研究为冠状病毒感染治疗提供了候选药物，为BLX的进一步开发应用提供了理论参考。

Abstract

Objective

To investigate the effects of thyme herbal tea （BLX） on the proliferation of human coronavirus OC43 （HCoV-OC43）

in vitro

and

in vivo

Method

The chemical composition of BLX was analyzed by UPLC-MS. The cytotoxicity of BLX in HRT-18 cells and the effect of BLX treatment on the proliferation of HCoV-OC43 in cells were analyzed. Copies of viral gene were detected by real-time PCR. The effect of BLX treatment on the life cycle of HCoV-OC43 was detected by time-of-addition assay. The maximum tolerated dose of BLX and the influences of BLX on the body weight and survival time of suckling mice infected with HCoV-OC43 were determined. The expression of viral protein in the brain and lung tissue was analyzed by immunohistochemistry.

Result

There were 11 chemical components identified in BLX by UPLC-MS. BLX showed the 50% cytotoxic concentration （CC

） of （13 859.56±319） mg·L

-1

， the median inhibitory concentration （IC

） of （1 439.09±200） mg·L

-1

， and the selection index of 8.26-11.44 for HCoV-OC43 in HRT-18 cells. Compared with the cells infected with HCoV-OC43， BLX at the concentrations of 1 500， 1 000， 500 mg·L

-1

inhibited the proliferation of this virus （

0.05，

0.01）. BLX exhibited antiviral effect in the early stage of virus infection， and the inhibition role in the attachment stage was more significant than that in the entry stage （

0.05）. In the suckling mice infected with HCoV-OC43， BLX at 1200 and 600 mg·kg

-1

·d

-1

alleviated the symptoms， prolonged the survival period， reduced the death rate， and down-regulated the mRNA level of nucleocapsid protein in the mice. Moreover， BLX at 1 2

00 mg·kg

-1

·d

-1

down-regulated the expression of nucleocapsid protein in the brain （

0.01） and the lung （

0.01）.

Conclusion

BLX contained multiple antiviral ingredients. It inhibited the proliferation of HCoV-OC43 both

in vitro

and

in vivo

by interference with viral attachment. This study provides theoretical reference for the treatment of acute respiratory tract infection with HCoV-OC43 and for further development and application of BLX.

关键词

Keywords

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Effect of Thyme Herbal Tea on Proliferation of Human Coronavirus OC43 in vitro and in vivo

DOI：10.13422/j.cnki.syfjx.20241504

摘要

Abstract

关键词

Keywords

references