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1.成都中医药大学 药学院,成都 611137
2.重庆三峡学院 生物与食品工程学院,重庆 404020
3.重庆三峡学院 数学与统计学院,重庆 404020
Received:07 November 2024,
Accepted:16 December 2024,
Published Online:18 December 2024,
Published:20 July 2025
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盘付梅,施志芬,王凯等.基于最小数据集的解磷细菌对太白贝母土壤肥力的影响[J].中国实验方剂学杂志,2025,31(14):164-170.
PAN Fumei,SHI Zhifen,WANG Kai,et al.Evaluation of Effect of Phosphorus-solubilizing Bacteria on Soil Fertility of Fritillaria taipaiensis Based on Minimum Data Set[J].Chinese Journal of Experimental Traditional Medical Formulae,2025,31(14):164-170.
盘付梅,施志芬,王凯等.基于最小数据集的解磷细菌对太白贝母土壤肥力的影响[J].中国实验方剂学杂志,2025,31(14):164-170. DOI: 10.13422/j.cnki.syfjx.20241818.
PAN Fumei,SHI Zhifen,WANG Kai,et al.Evaluation of Effect of Phosphorus-solubilizing Bacteria on Soil Fertility of Fritillaria taipaiensis Based on Minimum Data Set[J].Chinese Journal of Experimental Traditional Medical Formulae,2025,31(14):164-170. DOI: 10.13422/j.cnki.syfjx.20241818.
目的
2
探究不同解磷细菌及其组合对太白贝母土壤肥力的影响,为其种植和菌肥开发提供参考。
方法
2
设6个接菌组和1个空白组(CK组),取栽培土壤测定有机质、速效磷、速效钾等17个指标,通过建立最小数据集(MDS)筛出最敏感指标评价其土壤肥力。
结果
2
与CK组比较,不同解磷细菌处理组的土壤有机质、速效钾、速效磷、细菌数量、放线菌数量、蔗糖酶活性等有不同程度增加,筛选出细菌数量、速效磷、真菌数量、碱性磷酸酶共4个指标构建MDS。隶属度雷达图显示,各施菌组的肥力评价优于CK组,接菌组Y12组(YP5-1和YP3-1,蜡状芽孢杆菌)>W12组(WP7-2蜡状芽孢杆菌和WP2-2普城沙雷氏菌)>Y2组(YP5-1蜡状芽孢杆菌)>W2组(WP2-2普城沙雷氏菌)>Y1组(YP3-1蜡状芽孢杆菌)>W1组(WP7-2蜡状芽孢杆菌)>CK组(蒸馏水),即Y12组土壤肥力水平最高,CK组最低。总数据集土壤质量指数与MDS质量指数呈正相关,表明可用MDS代替总数据集对太白贝母栽培土壤肥力进行评价。聚类结果显示,土壤肥力分为4个等级,且主要分布在Ⅱ(良)、Ⅲ(一般)级,共有15个样本属于这两级,有3个样本属于Ⅰ级(优),分布于Y12组,3个样本属于Ⅳ级(差),分布于CK组。
结论
2
施加不同解磷细菌均可提升太白贝母土壤肥力,增加营养元素含量和有益酶活性,改善微生物群落结构,组合菌组(Y12组、W12组)的土壤肥力水平均高于对应的单一菌组,但速效氮处于缺乏状态,栽培过程中需施加适量氮肥。
Objective
2
To investigate the effects of various phosphorus-solubilizing bacteria and their combinations on the soil fertility of
Fritillaria
taipaiensis
and to furnish a reference for the cultivation of this plant and the development of bacterial fertilizer.
Methods
2
A total of 17 indicators,such as organic matter,available phosphorus and available potassium,were determined for the cultivated soil samples from 6 bacterial groups and a blank(CK) group. A minimum data set(MDS) was established to screen the most sensitive indicators for evaluating soil fertility.
Results
2
Compared with the CK group,phosphorus-solubilizing bacteria treatment groups presented i
ncreases in soil organic matter,available potassium,available phosphorus,bacterial count,actinomycetes count,and sucrase activity. The MDS was established with four indicators: Bacterial count,available phosphorus,fungal count,and alkaline phosphatase activity. The membership radar map indicated that the soil fertility of each fertilization group was superior to that of the CK group,following the trend of Y12(
Bacillus cereus
YP5-1 and YP3-1)
>
W12(
B. cereus
WP7-2 and
Serratia plymuthica
WP2-2)
>
Y2(
B. cereus
YP5-1)
>
W2(
Serratia plymuthica
WP2-2)
>
Y1(
B. cereus
YP3-1)
>
W1(
B. cereus
WP7-2)
>
CK(distilled water). The soil fertility was the highest in the Y12 group and the lowest in the CK group. The soil quality index of the total data set was positively correlated with that of MDS,suggesting that the MDS could be utilized in place of the total data set to evaluate the soil fertility of
F. taipaiensis
. The clustering outcomes demonstrated that the cultivated soil fertility of
F. taipaiensis
was classified into 4 grades,with most(15) samples in grades Ⅱ(good) and Ⅲ(medium). In addition,3 samples in the Y12 group showed the soil fertility of grade Ⅰ(excellent) and 3 samples in the CK group showed the soil fertility of grade Ⅳ(poor).
Conclusion
2
The application of different phosphorus-solubilizing bacteria can enhance soil fertility,facilitate nutrient circulation,increase beneficial enzyme activity,and improve the microbial community structure. The soil fertility of the bacterial combination groups Y12 and W12 is higher than that of the corresponding single bacterial strain groups. However,the content of availabe nitrogen in the combination groups is insufficient,and appropriate nitrogen fertilizer should be applied during the cultivation process.
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