Isolation of Growth-Promoting Bacteria and Effect of Compound Bacteria on Yield of <italic style="font-style: italic">Fritillaria przewalskii</italic>

Jiang ZHAO; Shi-jun LIANG; Tao YANG; Xiao-long WU; Peng-nian QI; Shi-wei WANG; Zhi-ye WANG

doi:10.13422/j.cnki.syfjx.20211512

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Isolation of Growth-Promoting Bacteria and Effect of Compound Bacteria on Yield of Fritillaria przewalskii

更新时间：2021-11-12

- Isolation of Growth-Promoting Bacteria and Effect of Compound Bacteria on Yield of Fritillaria przewalskii
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 24, Pages: 163-170(2021)
- 作者机构：
  
  1.甘肃省科学院生物研究所，兰州 730000
  2.华亭市中药材产业发展服务中心，甘肃华亭 744100
  3.甘肃省微生物资源开发利用重点实验室，兰州 730000
  4.甘肃绿能农业科技股份有限公司，甘肃武威 733000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20211512
  CLC： R931;R28;Q93;O657.7
- Published：20 December 2021，
  
  Published Online：25 October 2021，
  
  Received：19 August 2021，
- 稿件说明：
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赵疆,梁世军,杨涛等.促生细菌的分离及复配菌剂对甘肃贝母产量的影响[J].中国实验方剂学杂志,2021,27(24):163-170.

ZHAO Jiang,LIANG Shi-jun,YANG Tao,et al.Isolation of Growth-Promoting Bacteria and Effect of Compound Bacteria on Yield of Fritillaria przewalskii[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(24):163-170.
赵疆,梁世军,杨涛等.促生细菌的分离及复配菌剂对甘肃贝母产量的影响[J].中国实验方剂学杂志,2021,27(24):163-170. DOI： 10.13422/j.cnki.syfjx.20211512.

ZHAO Jiang,LIANG Shi-jun,YANG Tao,et al.Isolation of Growth-Promoting Bacteria and Effect of Compound Bacteria on Yield of Fritillaria przewalskii[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(24):163-170. DOI： 10.13422/j.cnki.syfjx.20211512.

摘要

目的

研究叶片喷施厚壁菌门芽孢杆菌属复合菌剂（T1），假单孢菌属和根瘤菌属复合菌剂（T2）两类复配促生菌剂对甘肃贝母生理特征与生长的影响，以期为甘肃贝母生态种植开发功能性微生物菌剂奠定基础。

方法

采用常规方法分离鉴定出甘肃贝母内生细菌；对3年生甘肃贝母叶面喷施T1和T2，并测定产量；试剂盒法测定植物和微生物相关酶活性及生理生化指标；液相色谱-质谱联用技术（LC-MS）检测植物和微生物相关激素含量。

结果

从甘肃贝母中分离到的内生细菌分属于厚壁菌门、变形菌门、放线菌门；T2处理的超氧化物歧化酶（SOD），过氧化物酶（POD）活性及生长素含量显著高于T1处理，T1处理的嗜铁素、水杨酸、赤霉素含量显著高于T2处理；TI和T2处理较空白组（CK）提高了贝母叶片内源赤霉素、细胞分裂素、生长素含量，茉莉酸、脱落酸差异无统计学意义，T1处理促进了贝母叶片内源水杨酸的积累，T2处理差异无统计学意义；TI和T2处理较CK提高了贝母叶片SOD，POD，过氧化氢酶（CAT）活性，降低了丙二醛含量，T2处理促进了贝母叶片过氧化氢的积累，T1处理差异无统计学意义；TI和T2处理较CK提高了贝母叶绿素、根际土铁平均含量及百株重。

结论

T1，T2处理都有促进增产的作用，具体机制有所区别，T1优于T2处理。

Abstract

Objective

To explore the effects of anti-microbial compound （T1） from

Bacillus

（Phylum Firmicutes） and anti-microbial compound （T2） from

Pseudomonas

and

Rhizobium

， two growth-promoting agents， on the physiological characteristics and growth of

Fritillaria przewalskii

， in order to lay a foundation for the development of functional microbial agents and the promotion of ecological planting.

Method

The endophytic bacteria of

F. przewalskii

were isolated and identified using conventional methods. The leaves of three-year-old

F. przewalskii

were sprayed with T1 and T2， followed by yield determination. The enzyme activities and physiological and biochemical indexes in the plant and microorganisms were measured using the corresponding assay kits， and the contents of related hormones by liquid chromatography-mass spectrometry （LC-MS）.

Result

The isolated endophytic bacteria were classified into Firmicutes，Proteobacteria， and Actinomycetes. The activities of superoxide dismutase（SOD） and peroxidase（POD） and auxin content after T2 treatment were significantly higher than those after T1 treatment， while the contents of siderophore，salicylic acid， and gibberellin were lower. Compared with the blank （CK） group， T1 and T2 increased the contents of endogenous gibberellin，cytokinin， and auxin in

F. przewalskii

leaves，but did not significantly change jasmonic acid and abscisic acid. T1 promoted the accumulation of endogenous salicylic acid in

F. przewalskii

leaves， but there was no significant change after T2 treatment. Compared with CK，T1 and T2 enhanced the activities of SOD， POD， and catalase （CAT） and decreased the content of malondialdehyde. T2 promoted the accumulation of hydrogen peroxide in

F. przewalskii

leaves， but no significant difference was observed after T1 treatment. Compared with CK，both T1 and T2 increased chlorophyll，average iron content in rhizosphere soil， and 100-plant weight.

Conclusion

T1 and T2 treatments help to increase the yield，and their specific mechanisms differ from each other. T1 exhibits better effect than T2.

关键词

甘肃贝母芽孢杆菌属假单孢菌属促生作用

Keywords

Fritillaria przewalskiiBacillusPseudomonasgrowth-promoting effect

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Related Institution

College of Pharmacy，Gansu University of Chinese Medicine

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Northwest Collaborative Innovation Center of Traditional Chinese Medicine （TCM） and Tibetan Medicine， Gansu Key Laboratory Breeding Base for TCM Quality and Standard Research

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Laboratory of Nanometer Applied Technology，Gansu Academy of Sciences

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Isolation of Growth-Promoting Bacteria and Effect of Compound Bacteria on Yield of Fritillaria przewalskii

DOI：10.13422/j.cnki.syfjx.20211512

摘要

Abstract

关键词

Keywords

references