Inhibitory Effect and Mechanism of Jingulian Capsule on Human Breast Cancer MDA-MB-231 Cells

Jian-fei QIU; Jue YANG; Zhi-yin ZHANG; Wu-ling LIU; Hui SONG; Xiao-sen WU; Jing LI; Yan-mei LI

doi:10.13422/j.cnki.syfjx.20212424

您当前的位置：

首页 >

文章列表页 >

Inhibitory Effect and Mechanism of Jingulian Capsule on Human Breast Cancer MDA-MB-231 Cells

更新时间：2021-11-12

- Inhibitory Effect and Mechanism of Jingulian Capsule on Human Breast Cancer MDA-MB-231 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 24, Pages: 78-83(2021)
- 作者机构：
  
  1.贵州医科大学省部共建药用植物功效与利用国家重点实验室，贵州省中国科学院天然产物化学重点实验室，贵阳 550014
  2.贵州医科大学大学城医院，贵阳 550025
  3.贵航贵阳医院，贵阳 550027
  4.湖南省湘西自治州烟草公司永顺县分公司，湖南湘西自治州 416000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20212424
  CLC： R22;R242;R2-031;R285.5
- Published：20 December 2021，
  
  Published Online：26 October 2021，
  
  Received：31 August 2021，
- 稿件说明：
扫描看全文
邱剑飞,杨珏,张知音等.金骨莲胶囊对人乳腺癌MDA-MB-231细胞的抑制作用及机制[J].中国实验方剂学杂志,2021,27(24):78-83.

QIU Jian-fei,YANG Jue,ZHANG Zhi-yin,et al.Inhibitory Effect and Mechanism of Jingulian Capsule on Human Breast Cancer MDA-MB-231 Cells[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(24):78-83.
邱剑飞,杨珏,张知音等.金骨莲胶囊对人乳腺癌MDA-MB-231细胞的抑制作用及机制[J].中国实验方剂学杂志,2021,27(24):78-83. DOI： 10.13422/j.cnki.syfjx.20212424.

QIU Jian-fei,YANG Jue,ZHANG Zhi-yin,et al.Inhibitory Effect and Mechanism of Jingulian Capsule on Human Breast Cancer MDA-MB-231 Cells[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(24):78-83. DOI： 10.13422/j.cnki.syfjx.20212424.

摘要

目的

研究金骨莲胶囊对人三阴性乳腺癌（TNBC）细胞MDA-MB-231增殖的影响，探讨金骨莲胶囊对TNBC的治疗作用机制。

方法

利用超高效液相色谱-质谱联用（UPLC-MS/MS）技术，对金骨莲胶囊的成分进行分析鉴定。通过用噻唑蓝（MTT）比色法观察金骨莲胶囊（0.125，0.25，0.5，1，2 g·L

-1

）对人乳腺癌细胞MDA-MB-231的增殖抑制作用。采用Hoechst 33258染色检测金骨莲胶囊处理MDA-MB-231细胞24 h后的细胞核凋亡形态学的变化。采用流式细胞仪检测金骨莲胶囊不同质量浓度和作用时间对MDA-MB-231细胞凋亡及周期分布情况。通过蛋白免疫印迹法（Western blot）检测各组细胞多聚二磷酸腺苷核糖聚合酶（PARP），原癌基因蛋白（c-Myc），细胞周期蛋白B

（cyclin B

）和磷酸化胞外信号调节激酶（p-ERK）的蛋白表达水平。

结果

该研究利用UPLC-MS/MS技术鉴定了金骨莲胶囊提取物中113个化合物成分。MTT比色法显示，与空白组比较，金骨莲胶囊组（0.125，0.25，0.5，1，2 g·L

-1

）细胞抑制率显著增加（

0.01），半数抑制浓度（IC

）为（0.13±0.02）g·L

-1

；流式细胞术结果显示，与空白组比较，金骨莲胶囊组（1 g·L

-1

）细胞凋亡率显著升高（

0.01），金骨莲胶囊组（0.5，1 g·L

-1

）细胞处于S期的数量明显增加（

0.05，

0.01）；Western blot结果显示，与空白组比较，金骨莲胶囊组（0.5，1 g·L

-1

）细胞中的PARP，c-Myc，cyclin B

蛋白表达水平显著降低（

0.01），金骨莲胶囊组（1 g·L

-1

）p-ERK蛋白表达水平显著降低（

0.01）。

结论

金骨莲胶囊可抑制MDA-MB-231细胞增殖，使细胞周期阻滞于S期，并诱导其凋亡，其机制可能与其抑制MDA-MB-231细胞MAPK信号通路有关。

Abstract

Objective

To observe effect of Jingulian capsule on the proliferation of human breast cancer MDA-MB-231 cells and investigate its action mechanism against triple negative breast cancer （TNBC）.

Method

The ingredients of Jingulian capsule were identified by ultra-performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）. The inhibitory effect of Jingulian capsule at different doses （0.125，0.25，0.5，1，and 2 g·L

-1

） against the proliferation of MDA-MB-231 cells were detected by methyl thiazolyl tetrazolium （MTT） assay. After treatment for 24 h， the morphological changes in nuclear apoptosis of MDA-MB-231 cells were detected by Hoechst 33258 staining. The effect of different concentrations of Jingulian capsule on the apoptosis and cycle of MDA-MB-231 cells after different treatment time were determined by flow cytometry. The protein expression levels of Poly-ADP-ribose polymeras （PARP）， proto-oncogene c-Myc， cyclin B

， and phosphorylated extracellular signal-regulated kinase （p-ERK） in each group were assayed by Western blot.

Result

A total of 113 compounds in Jingulian capsule were identified by UPLC-MS/MS. As revealed by MTT assay，compared with blank group，Jingulian capsule （0.125，0.25，0.5，1，2 g·L

-1

） significantly inhibited viability of MDA-MB-231 cells （

0.01）， with the half maximal inhibitory concentration （ IC

） of（0.13±0.02）g·L

-1

. According to flow cytometry，compared with the blank group，Jingulian capsule at 1 g·L

-1

significantly induced the apoptosis of MDA-MB-231 cells （

0.05）and Jingulian capsule at 0.5， 1 g·L

-1

obviously increased the number of MDA-MB-231 cells in S phase （

0.05，

0.01）. The results of Western blotting demonstrated that the protein expression levels of PARP，c-Myc，and cyclin B

in 0.5， 1 g·L

-1

Jingulian capsule groups were remarkably down-regulated as compared with those in the blank group（

0.01）， and the protein expression level of p-ERK in 1 g·L

-1

Jingulian capsule group was also down-regulated （

0.01）.

Conclusion

Jingulian capsule is able to inhibit the proliferation of MDA-MB-231 cells，induce S phase cell cycle arrest， and promote their apoptosis， which may be related to the inactivation of the MAPK signaling pathway.

关键词

金骨莲胶囊MDA-MB-231细胞凋亡S期阻滞

Keywords

Jingulian capsuleMDA-MB-231 cellsapoptosisS phase arrest

references

SIEGEL R L，MILLER K D，JEMAL A. Cancer statistics［J］. CA Cancer J Clin，2019，69（1）：7-34.

SERRA K P，RAMALHO S，TORRESAN R，et al. Nova classificação dos carcinomas da mama：procurando luminal A ［J］. Rev Bras Ginecol Obstet，2014，36（12）：575-580.

BEAUPARLANT P，SHORE G C. Therapeutic activation of caspases in cancer：a question of selectivity［J］. Curr Opin Drug Discov Devel，2003，6（2）：179-187.

吴雨洁，刘敏，高静东.三阴性乳腺癌分子靶向治疗研究进展［J］.中南医学科学杂志，2019，47（2）：217-220.

ELSAMANY S， ABDULLAH S. Triple-negative breast cancer： future prospects in diagnosis and management［J］. Med Oncol，2014，31（2）：834.

王晋慧，吕邵娃，李孟，等.中药有效成分体外抗乳腺癌作用机制的研究进展［J］.中国实验方剂学杂志，2020，26（17）：197-203.

李云祥，梁引库，高飞雄，等.中药治疗乳腺癌疾病研究进展［J］.中国实验方剂学杂志，2019，25（3）：211-219.

吴沁航，朱丽文，李铭轩，等.中药抗三阴性乳腺癌及其分子作用机制研究进展［J］.南京中医药大学学报，2021（4）：602-608.

李思翰，练东银，张广平，等. 基于PI3K/Akt信号通路探讨金骨莲提取物抑制脂多糖诱导RAW264.7细胞炎症反应的作用及机制［J］. 中国实验方剂学杂志，2021，27（14）：29-35.

彭旭，杨继滨，尤奇，等.金骨莲灌胃对兔骨关节炎模型软骨细胞凋亡相关蛋白表达的影响［J］.中国组织工程研究，2020，24（8）：1188-1194.

阮寅正，甘甜，叶泽耀.苦参碱调节胃癌细胞糖代谢改变其生物学特性的机制［J］.中国临床药理学杂志，2021，37（10）：1201-1204.

简白羽，岳丽玲，韩翠艳，等.没食子酸乙酯通过线粒体通路诱导人结肠癌LoVo细胞凋亡的机制研究［J］.中国药学杂志，2016，51（6）：463-467.

李伟.金骨莲胶囊治疗骨性关节炎的临床观察［J］.中医临床研究，2019，11（1）：75-77.

王胜民，刘毅，刘波，等.金骨莲胶囊对兔膝骨关节炎软骨保护作用的研究［J］.重庆医学，2016，45（26）：3611-3615.

BAI P，CANTÓ C. The role of PARP-1 and PARP-2 enzymes in metabolic regulation and disease［J］. Cell Metab，2012，16（3）：290-295.

O'SHAUGHNESSY J，OSBORNE C，PIPPEN J E，et al. Iniparib plus chemotherapy in metastatic triple-negative breast cancer［J］. N Engl J Med，2011，364（3）：205-214.

李常有，于志强，崔军威，等.c-Myc蛋白对三阴性乳腺癌增殖迁移的影响［J］.暨南大学学报：自然科学与医学版，2019，40（2）：129-134.

STRAUSS B，HARRISON A，COELHO P A，et al. Cyclin B1 is essential for mitosis in mouse embryos，and its nuclear export sets the time for mitosis. ［J］ Cell Biol，2018，217（1）：179-193.

ARTHUR J S C，LEY S C. Mitogen-activated protein kinases in innate immunity［J］. Nat Rev Immunol，2013，13（9）：679-692.

QIU S，HU W，MA Q，et al. TIPE1 suppresses the invasion and migration of breast cancer cells and inhibits epithelial-to-mesenchymal transition primarily via the ERK signaling pathway［J］. Acta Biochim Biophys Sin （Shanghai），2019，51（10）：1008-1015.

YEH H T，TSAI Y S，CHEN M S，et al. Flavopereirine induces cell cycle arrest and apoptosis via the Akt/p38 MAPK/ERK1/2 signaling pathway in human breast cancer cells［J］. Eur J Pharmacol，2019，863：172658.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Curdione on MDA-MB-231 Cell Cycle and Apoptosis

Computer-aided Drug Design and Experimental Validation Reveal Molecular Mechanism of Saikosaponin D-induced Apoptosis of Bladder Cancer Cells

Mechanism of Culuan Heji in Regulating AMPK/mTOR/HIF-1/VEGF Pathway to Improve Oxidative Stress and Apoptosis in Rats with Follicular Maldevelopment

Effect of Haizao Yuhutang Combined with Modified Sargassum and Glycyrrhizae Radix et Rhizoma on Expression of p53/p21/Caspase-3 Pathway in Rats with Goiter

Effect of Xiaoyaosan on Apoptosis in Mouse Model of Lipopolysaccharide-induced Depressive-like Behavior in Mice

Related Author

Kai-yuan ZHANG

Ling-ling LYU

Jing-xian CHEN

Jia-yue XU

Qiong LI

Yuan WU

Lan ZHENG

ZUO Ling

Related Institution

Ruijin Hospital，Shanghai Jiaotong University School of Medicine

The Second Affiliated Hospital of Guangdong Medical University

Affiliated Hospital of Guangdong Medical University

Fujian University of Traditional Chinese Medicine（TCM）

Research Base of TCM Syndrome of Fujian University of TCM

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰