Mechanism of Cordycepin Inhibiting Proliferation and Promoting Apoptosis of Hepatoma Cells by Regulating Gli1

Wang-xia TONG; Ning LUO; Gui-feng LI; Xu-dong LIU; Xuan LIU; Kai-meng WANG

doi:10.13422/j.cnki.syfjx.20220295

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Mechanism of Cordycepin Inhibiting Proliferation and Promoting Apoptosis of Hepatoma Cells by Regulating Gli1

更新时间：2021-12-14

- Mechanism of Cordycepin Inhibiting Proliferation and Promoting Apoptosis of Hepatoma Cells by Regulating Gli1
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 28, Issue 2, Pages: 104-111(2022)
- 作者机构：
  
  广西中医药大学附属瑞康医院，南宁 530011
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20220295
  CLC： R22;R242;R285.5;R2-031;R979.1
- Published：20 January 2022，
  
  Published Online：17 November 2021，
  
  Received：04 January 2021，
- 稿件说明：
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童汪霞,罗宁,李桂凤等.虫草素通过调控Gli1介导抗肝癌细胞增殖及促凋亡的机制[J].中国实验方剂学杂志,2022,28(02):104-111.

TONG Wang-xia,LUO Ning,LI Gui-feng,et al.Mechanism of Cordycepin Inhibiting Proliferation and Promoting Apoptosis of Hepatoma Cells by Regulating Gli1[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(02):104-111.
童汪霞,罗宁,李桂凤等.虫草素通过调控Gli1介导抗肝癌细胞增殖及促凋亡的机制[J].中国实验方剂学杂志,2022,28(02):104-111. DOI： 10.13422/j.cnki.syfjx.20220295.

TONG Wang-xia,LUO Ning,LI Gui-feng,et al.Mechanism of Cordycepin Inhibiting Proliferation and Promoting Apoptosis of Hepatoma Cells by Regulating Gli1[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(02):104-111. DOI： 10.13422/j.cnki.syfjx.20220295.

摘要

目的

探讨虫草素抑制人肝癌细胞增殖并促其凋亡的分子机制。

方法

采用干扰小RNA（siRNA）技术沉默胶质肿瘤相关癌基因同源物1（Gli1）基因并转染SMMC-7721细胞，细胞增殖与活性检测（CCK-8）及细胞克隆实验检测细胞增殖能力；使用不同剂量的虫草素对肝癌细胞进行干预，检测肝癌细胞的增殖、凋亡情况。使用实时荧光定量聚合酶链式反应（Real-time PCR）及蛋白免疫印迹法（Western blot）检测Gli1及其下游相关基因的表达情况。

结果

与正常肝细胞比较，Gli1在SMMC-7721肝癌细胞中的mRNA及蛋白表达水平显著升高（

0.01）。CCK-8及克隆形成实验结果显示，与空白组比较，干扰沉默Gli1组SMMC-7721细胞72，96 h的增殖率降低（

0.05，

0.01）；细胞集落形成能力显著降低（

0.01）。虫草素干预后，与空白组比较，虫草素80，120 μmol·L

-1

组72，96 h肝癌细胞的增殖明显降低（

0.05，

0.01）。凋亡实验结果显示，与空白组比较，虫草素80，120 μmol·L

-1

组SMMC-7721肝癌细胞凋亡显著增加（

0.01）。Real-time PCR及Western blot实验显示，虫草素80，120 μmol·L

-1

组Gli1在SMMC7721细胞中的mRNA及蛋白表达水平明显降低（

0.05，

0.01）；虫草素120 μmol·L

-1

组B细胞淋巴瘤-2（Bcl-2）和原癌基因（c-Myc） mRNA和蛋白表达降低（

0.05，

0.01），半胱氨酸天冬氨酸蛋白水解酶-3（Caspase-3） mRNA和蛋白表达增加（

0.05）。

结论

Gli1在肝癌中高表达。虫草素可通过对Gli1及其下游与凋亡相关因子的调控从而抑制肝癌增殖并促肝癌细胞凋亡。

Abstract

Objective

To investigate the molecular mechanism of cordycepin inhibiting proliferation and promoting apoptosis of human hepatoma cells （HCCs）.

Method

Glioma-associated oncogene homolog 1 （Gli1） gene was silenced by small interfering RNA （siRNA） and transfected into SMMC-7721 cells， and then cell proliferation was detected by cell counting kit-8 （CCK-8） assay and cell cloning assay. SMMC-7721 cells were treated with different concentration of cordycepin， and the cell proliferation and apoptosis were examined. The expression of Gli1 and the downstream related genes was determined by Real-time polymerase chain reaction（PCR） and Western blot.

Result

The mRNA and protein expression of Gli1 in SMMC-7721 cells was higher than that in normal liver cells （

0.01）. The proliferation rate of SMMC-7721 with silenced Gli1 decreased at 72 and 96 h （

0.05，

0.01）， and the colony-forming capacity lowered （

0.01） compared with those in the blank group. Compared with the control， 80 μmol·L

-1

and 120 μmol·L

-1

cordycepin significantly inhibited the proliferation of SMMC-7721 cells at 72 and 96 h （

0.05，

0.01）， and promoted the apoptosis of them （

0.01）. Moreover， 80 and 120 μmol·L

-1

cordycepin restrained the mRNA and protein expression of Gli1 in SMMC7721 cells （

0.05，

0.01）. At 120 μmol·L

-1

， cordycepin led to the decrease in the mRNA and protein levels of B-cell lymphoma-2 （Bcl-2） and c-Myc （

0.05，

0.01）， and the increase in the mRNA and protein expression of cysteine-aspartic acid protease-3 （Caspase-3）（

0.05）.

Conclusion

Gli1 is highly expressed in HCCs， and cordycepin can suppress the proliferation and enhance the apoptosis of HCCs by regulating Gli1 and the downstream apoptosis-related factors.

关键词

原发性肝癌虫草素胶质瘤相关肿瘤基因同源物1（Gli1）细胞增殖凋亡

Keywords

primary hepatic carcinomacordycepinglioma-associated oncogene homolog 1（Gli1）cell proliferationapoptosis

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