Effect of Buyang Huanwutang on Rehabilitation of Ischemic Stroke by Cell Membrane Solid-phase Chromatography Combined with Network Pharmacology

Xiang-li YAN; Ying-ying HE; Ming BAI; Er-ping XU

doi:10.13422/j.cnki.syfjx.20220111

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Effect of Buyang Huanwutang on Rehabilitation of Ischemic Stroke by Cell Membrane Solid-phase Chromatography Combined with Network Pharmacology

更新时间：2021-12-14

- Effect of Buyang Huanwutang on Rehabilitation of Ischemic Stroke by Cell Membrane Solid-phase Chromatography Combined with Network Pharmacology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 28, Issue 2, Pages: 191-198(2022)
- 作者机构：
  
  1.河南中医药大学中医药科学院，郑州 450046
  2.河南省仲景方药现代研究重点实验室，郑州 450046
  3.广州中医药大学中药学院，广州 510006
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20220111
  CLC： R284.2;R289;R22;R2-031;R33
- Published：20 January 2022，
  
  Published Online：26 November 2021，
  
  Received：16 July 2021，
- 稿件说明：
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闫向丽,贺颖颖,白明等.细胞膜固相色谱法联合网络药理学探讨补阳还五汤促进缺血性脑卒中康复的作用[J].中国实验方剂学杂志,2022,28(02):191-198.

YAN Xiang-li,HE Ying-ying,BAI Ming,et al.Effect of Buyang Huanwutang on Rehabilitation of Ischemic Stroke by Cell Membrane Solid-phase Chromatography Combined with Network Pharmacology[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(02):191-198.
闫向丽,贺颖颖,白明等.细胞膜固相色谱法联合网络药理学探讨补阳还五汤促进缺血性脑卒中康复的作用[J].中国实验方剂学杂志,2022,28(02):191-198. DOI： 10.13422/j.cnki.syfjx.20220111.

YAN Xiang-li,HE Ying-ying,BAI Ming,et al.Effect of Buyang Huanwutang on Rehabilitation of Ischemic Stroke by Cell Membrane Solid-phase Chromatography Combined with Network Pharmacology[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(02):191-198. DOI： 10.13422/j.cnki.syfjx.20220111.

摘要

目的

采用细胞膜固相色谱法联合网络药理学探讨补阳还五汤促进缺血性脑卒中康复的作用机制。

方法

细胞膜固相色谱法筛选补阳还五汤供试液中与海马神经元细胞特异性结合的成分；检索PubChem，PharmMapper，在线人类孟德尔遗传数据库（OMIM），GeneCards等数据库获取特异性结合成分的作用靶点及缺血性脑卒中疾病的靶点；借助STRING，Cytoscape 3.7.1软件绘制蛋白质-蛋白质相互作用（PPI）网络；利用DAVID在线数据库对PPI网络中的核心靶点进行基因本体（GO）功能富集分析及京都基因与基因组百科全书（KEGG）生物途径分析，探讨补阳还五汤促进缺血性脑中风康复可能的作用机制。

结果

通过细胞膜固相色谱法，筛选出了13个与海马神经元细胞特异性结合的成分；通过网络分析和靶点富集，显示作用靶点主要关联细胞增殖调控、蛋白质磷酸化、缺氧反应、血管生成等生物过程，对叉头转录因子（FoxO）信号通路、腺苷酸激活蛋白激酶（AMPK）信号通路、核转录因子-

B（NF-

B）信号通路、细胞凋亡通路等具有广泛的干预作用。

结论

补阳还五汤可能通过调节FoxO，AMPK，NF-

B，细胞凋亡等信号通路促进缺血性脑中风的康复。

Abstract

Objective

To explore the effect of Buyang Huanwutang （BHD） on rehabilitation of ischemic stroke（IS） by cell membrane solid-phase chromatography and network pharmacology.

Method

Cell membrane solid-phase chromatography was performed to screen the specific binding components of BHD with hippocampal neurons. Targets of the specific components were retrieved based on PubChem and PharmMapper and those of IS were searched from Online Mendelian Inheritance in Man （OMIM） and GeneCards. Then， the protein-protein interaction （PPI） network was constructed with STRING and Cytoscape 3.7.1， followed by Gene Ontology （GO） term enrichment and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway enrichment of the hub genes in the PPI network. Thereby， the mechanism of BHD in promoting IS rehabilitation was clarified.

Result

A total of 13 specific components were identified. The hub genes were mainly involved in the biological processes of regulation of cell proliferation， protein phosphorylation， hypoxia response， and angiogenesis， and the pathways of Forkhead box O （FoxO） signaling pathway， adenosine 5'-monophosphate （AMP）-activated protein kinase （AMPK） signaling pathway， nuclear factor kappa B （NF-

B） signaling pathway， and apoptosis pathway.

Conclusion

BHD may promote the recovery of IS by regulating FoxO， AMPK， NF-

B， and apoptosis pathways.

关键词

补阳还五汤缺血性脑中风细胞膜固相色谱法网络药理学

Keywords

Buyang Huanwutangischemic strokecell membrane solid-phase chromatographynetwork pharmacology

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