Therapeutic Effect and Antioxidant Mechanism of Xiaochuanning Granule on Psychological Stress Related Asthma in Rats

Dan HOU; Shuai-yang HUANG; Ming-sheng LYU; Xue-feng GONG; Hong-sheng CUI

doi:10.13422/j.cnki.syfjx.20212224

您当前的位置：

首页 >

文章列表页 >

Therapeutic Effect and Antioxidant Mechanism of Xiaochuanning Granule on Psychological Stress Related Asthma in Rats

更新时间：2021-12-14

- Therapeutic Effect and Antioxidant Mechanism of Xiaochuanning Granule on Psychological Stress Related Asthma in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 28, Issue 2, Pages: 55-61(2022)
- 作者机构：
  
  1.北京中医药大学第三附属医院，北京 100029
  2.北京中医药大学，北京 100029
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20212224
  CLC： R22;R242;R2-031;R285.5
- Published：20 January 2022，
  
  Published Online：28 September 2021，
  
  Received：26 July 2021，
- 稿件说明：
扫描看全文
候丹,黄帅阳,吕明圣等.哮喘宁颗粒对心理应激哮喘大鼠的治疗作用及抗氧化作用机制[J].中国实验方剂学杂志,2022,28(02):55-61.

HOU Dan,HUANG Shuai-yang,LYU Ming-sheng,et al.Therapeutic Effect and Antioxidant Mechanism of Xiaochuanning Granule on Psychological Stress Related Asthma in Rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(02):55-61.
候丹,黄帅阳,吕明圣等.哮喘宁颗粒对心理应激哮喘大鼠的治疗作用及抗氧化作用机制[J].中国实验方剂学杂志,2022,28(02):55-61. DOI： 10.13422/j.cnki.syfjx.20212224.

HOU Dan,HUANG Shuai-yang,LYU Ming-sheng,et al.Therapeutic Effect and Antioxidant Mechanism of Xiaochuanning Granule on Psychological Stress Related Asthma in Rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(02):55-61. DOI： 10.13422/j.cnki.syfjx.20212224.

摘要

目的

观察哮喘宁颗粒对心理应激性哮喘大鼠的治疗作用及抗氧化机制。

方法

6周龄雄性SD大鼠随机分为正常组、哮喘组、应激组、应激哮喘组、中药组（哮喘宁颗粒2.48 g·g

-1

灌胃）、西药组（布地奈德混悬液雾化）；第1，8天腹腔注射10%卵蛋白（OVA），氢氧化铝混合液+第15天开始1%OVA溶液雾化激发建立哮喘模型；其中应激组、应激哮喘组、中药组、西药组从第1天起给予束缚刺激，连续刺激28 d，最终建立心理应激性哮喘模型；第15天开始各组连续干预2周。第15，28天进行糖水偏好实验，旷场实验检测；实验结束，检测体质量，血清白细胞介素（IL）-4，IL-5，IL-13含量；肺组织丙二醛（MDA），超氧化物歧化酶（SOD），谷胱甘肽（GSH）水平；肺苏木素-伊红（HE）染色；蛋白免疫印迹法（Western blot）检测肺组织核因子E

相关因子2（Nrf2），血红素氧合酶-1（HO-1）蛋白表达。

结果

与应激哮喘组比较，中药组大鼠体质量、糖水消耗率、旷场路程明显升高（

0.05）；血清IL-4，IL-5，IL-13含量明显降低（

0.05）；肺组织SOD，GSH水平明显升高（

0.05），MDA水平明显降低（

0.05）；HE染色显示中药组支气管黏膜损伤较轻，炎细胞浸润、腺体增生、上皮变性坏死等明显减轻；与应激哮喘组比较，中药组肺组织Nrf2，HO-1蛋白表达明显升高（

0.05）。

结论

哮喘宁颗粒具有调节心理应激性哮喘大鼠心理应激状态作用，同时缓解气道炎症反应、抗氧化，与其上调Nrf2/HO-1蛋白表达相关。

Abstract

Objective

To observe the therapeutic effect and antioxidant mechanism of Xiaochuanning granule on psychological stress-related asthma in rats.

Method

The 6-week-old male SD rats were randomly divided into the normal group， asthma group， stress group， stress-related asthma group， western medicine group （atomization of budesonide suspension） and traditional Chinese medicine （TCM） group （Xiaochuanning granule 2.48 g·kg

-1

）. The asthma model was established during 28 days by intraperitoneal injection of 10% ovalbumin（OVA）on the 1

and 8

days and inhaling of vapourized 1% OVA started at the 15

day. Stress group， stress-related asthma group， western medicine group and TCM group were given restraint stimulation during the 28 days to establish the psychological stress-related asthma model. Rats in each group were administered with corresponding drug for 14 days from the 15

day. The sucrose preference test and open field test were performed at the 15

and 28

days. At the end of experiment， the body weight， serum interleukin-4 （IL-4）， interleukin-5 （IL-5） and interleukin-13 （IL-13） levels， as well as the levels of malondialdehyde （MDA）， superoxide dismutase （SOD） and glutathione （GSH） in lung tissues were detected by assay kits. Hematoxylin-eosin（HE） staining was conducted to observe the pathological changes in lung tissues. Meanwhile， Western blot was used to detect the protein expression of nuclear factor erythroid 2-related factor 2 （Nrf2） and hemeoxygenase-1 （HO-1） in lung tissues.

Result

Compared with the stress-related asthma group， the body weight， sugar water consumption rate and open field distance in the TCM group were significantly increased （

0.05）， and the serum IL-4， IL-5， IL-13 levels were significantly decreased （

0.05）， the levels of SOD and GSH in lung tissues increased significantly （

0.05）， while the level of MDA decreased significantly （

0.05）. HE staining showed that the bronchial mucosal injury， inflammatory cell infiltration， gland hyperplasia， epithelial degeneration and necrosis were significantly ameliorated in the TCM group than in the stress-related asthma group. The expression of Nrf2 and HO-1 protein in lung tissues also increased significantly （

0.05）.

Conclusion

Xiaochuanning Granule can regulate the psychological stress state of stress-related asthmatic rats， alleviate airway inflammatory reaction， and suppress oxidation， which is related to its up-regulation of the Nrf2/HO-1 protein expression.

关键词

哮喘宁颗粒心理应激哮喘大鼠心理应激氧化应激核因子E2相关因子2/血红素氧合酶1

Keywords

Xiaochuanning granulepsychological stress-related asthmatic ratspsychological stressoxidative stressnuclear factor erythroid 2-related factor 2/hemeoxygenase-1

references

MARSHALL J R. Psychological stress， immune dysfunction， and allergy： opportunities for improved patient health［J］. Ann Allergy Asthma Immunol，2020，125（4）：365-366.

范淑红，郝玉娥.过敏性哮喘患者心理应激与希望水平的相关性研究及其目标干预［J］.内蒙古医科大学学报，2020，42（S1）：36-39.

OHNO I. The interrel ationship between asthma and brain activities： psychological stress -related asthma as a new asthma phenotype［J］. Arerugi，2017，66（3）：153-160.

XU Z X， MENG L L， XIE Y J， et al. lncRNA pcgem1 strengthens anti-inflammatory and lung protective effects of montelukast sodium in children with cough-variant asthma［J］. Braz J Med Biol Res，2020，53（7）：e9271-e9281.

崔红生，靳锐锋，田彦.情志因素与支气管哮喘证治探析［J］.中华中医药杂志，2014，29（3）：771-773.

武维屏，崔红生.试论支气管哮喘从肝论治的生理病理学基础［J］.中国中医基础医学杂志，2002，8（10）：7-8.

弓雪峰，任培中，崔红生，等.基于网络药理学探讨哮喘宁颗粒防治心理应激哮喘的作用网络与潜在机制［J］.首都医科大学学报，2020，41（4）：576-589.

单敏敏，李长安，崔红生.哮喘宁颗粒对哮喘大鼠Th1/Th2平衡和STAT1通路调节作用的研究［J］.中国医药导报，2019，16（10）：11-15.

单敏敏，李长安，崔红生.哮喘宁颗粒对哮喘大鼠白三烯B4、白三烯C4、C-反应蛋白表达的调节作用［J］.世界中西医结合杂志，2019，14（1）：1-3，12.

CHEN Y Q， KONG Y， WANG Q L， et al. Schisandrin B attenuates airway inflammation by regulating the NF-κB/Nrf2 signaling pathway in mouse models of asthma［J］. J Immunol Res，2021，7（28）：8029963-8029972.

XIAN Z M， CHOI Y H， ZHENG M Y， et al. Imperatorin alleviates ROS-mediated airway remodeling by targeting the Nrf2/HO-1 signaling pathway ［J］. Biosci Biotechnol Biochem，2020，84（5）：898-910.

KAWANO T， OUCHI R， ISHIGAKI T， et al. Increased susceptibility to allergic asthma with the impairment of respiratory tolerance caused by psychological stress［J］. Int Arch Allergy Immunol， 2018， 177（1）：1-15.

李长安，胡晓玲，崔红生，等.心理应激哮喘大鼠模型的建立与评价［J］.北京中医药大学学报，2016，39（12）： 1033-1040.

李长安. 心理应激哮喘大鼠模型的建立及调肝理肺法作用机制研究［D］.北京：北京中医药大学，2017.

MIYASAKA T， DO K， TAKAHASHI T， et al. The interplay between neuroendocrine activity and psychological stress-induced exacerbation of allergic asthma［J］. Allergology International， 2018， 67（1）：32-42.

王一旭，李媛媛，张碧涛，等.逍遥散对慢性不可预知温和应激大鼠下丘脑弓状核JAK/STAT通路变化的影响［J］.中国实验方剂学杂志，2020，26（21）：21-27.

HU C， LUO Y， WANG H， et al. Re-evaluation of the interrelationships among the behavioral tests in rats exposed to chronic unpredictable mild stress ［J］. PLoS One， 2017， 12（9）：e0185129-e0185138.

JIN S， ZHAO Y， JIANG Y， et al. Anxiety-like behaviour assessments of adolescent rats after repeated maternal separation during early life［J］. Neuroreport，2018，29（8）：643-649.

HARTMANN B， LEUCHT V， LOERBROKS A. Work stress， asthma control and asthma-specific quality of life： initial evidence from a cross-sectional study［J］. J Asthma， 2017， 54（2）：210-216.

HUANG Y， HAO C， SU X. Neuroimmune regulation of lung infection and inflammation［J］. QJM， 2019， 112（7）：483-487.

崔红生，武维屏.武维屏辨治肺系疾病思路［J］.中医杂志，2013，54（2）：107-109.

崔红生.《内经》“和”思想在支气管哮喘防治中的意义［J］.北京中医药大学学报，2013，36（12）：802-804.

楚荷莹，王峰，白勇，张国俊.金雀异黄酮对幼龄哮喘大鼠TGF-β/Nrf2/HO-1信号通路及气道重塑的影响［J］.热带医学杂志，2021，21（5）：580-585，673.

YANG C C， YANG C M. Chinese herbs and repurposing old drugs as therapeutic agents in the regulation of oxidative stress and inflammation in pulmonary diseases［J］. J Inflamm Res，2021，4（14）：657-687.

徐畅，宋艺兰，姜京植，等.川芎嗪通过AMPK/NF-κB和Nrf-2/HO-1途径减轻过敏性气道炎症和氧化应激的实验研究［J］.免疫学杂志，2021，37（2）：100-106.

CHO H Y， KLEEBERGER S R. Nrf2 protects against airway disorders［J］. Toxicol Appl Pharmacol， 2010，244（1）：43-56.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Hei Xiaoyaosan Regulates RAS/RAF/MEK/ERK Signaling Pathway to Ameliorate Oxidative Stress in Rat Model of AD

Mechanism of Chinese Medicine Against Diabetic Gastroparesis： A Review

Mechanism of Culuan Heji in Regulating AMPK/mTOR/HIF-1/VEGF Pathway to Improve Oxidative Stress and Apoptosis in Rats with Follicular Maldevelopment

Effect of Modified Shengjiangsan on Renal Endoplasmic Reticulum Stress and Sirt1/PERK Pathway in Rat Model of Diabetic Nephropathy

Experimental Study on Regulation of Nrf2/HO-1 by Linalool to Inhibit Hepatic Injury Induced by Aflatoxin B₁

Related Author

WANG Huping

LYU Yujie

HU Yunyun

MENG Zhipeng

YANG Jiao

CHEN Yiqin

LI Long

TANG Zhifeng

Related Institution

Gansu University of Chinese Medicine

Gansu Province Key Laboratory of Traditional Chinese Medicine（TCM）Mining and Innovation Transformation

Gansu Provincial Laboratory of New TCM Product Creation Engineering

Tianshui Hospital of Integrated Traditional Chinese and Western Medicine

Tianshui Hospital of Traditional Chinese Medicine

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰