Establishment and Evaluation of Animal Model of Oligoasthenospermia

Tiansong SUN; Bonan LI; Qinghu HE

doi:10.13422/j.cnki.syfjx.20221496

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Establishment and Evaluation of Animal Model of Oligoasthenospermia

更新时间：2022-09-01

- Establishment and Evaluation of Animal Model of Oligoasthenospermia
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 28, Issue 14, Pages: 179-185(2022)
- 作者机构：
  
  1.湖南中医药大学中西医结合学院，长沙 410208
  2.湖南医药学院，湖南怀化 418002
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20221496
  CLC： R242;R2-0;R256.56;R697
- Published：20 July 2022，
  
  Published Online：22 March 2022，
  
  Received：12 January 2022，
- 稿件说明：
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孙天松,李波男,何清湖.少弱精子症动物模型建立方法及模型评价[J].中国实验方剂学杂志,2022,28(14):179-185.

SUN Tiansong,LI Bonan,HE Qinghu.Establishment and Evaluation of Animal Model of Oligoasthenospermia[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(14):179-185.
孙天松,李波男,何清湖.少弱精子症动物模型建立方法及模型评价[J].中国实验方剂学杂志,2022,28(14):179-185. DOI： 10.13422/j.cnki.syfjx.20221496.

SUN Tiansong,LI Bonan,HE Qinghu.Establishment and Evaluation of Animal Model of Oligoasthenospermia[J].Chinese Journal of Experimental Traditional Medical Formulae,2022,28(14):179-185. DOI： 10.13422/j.cnki.syfjx.20221496.

摘要

少、弱精子症是临床男性不育的主要类型，患者数量逐年升高且呈年轻化的趋势，其病因复杂发生机制尚未完全清楚，用药也多是经验性用药，因此构建合理的动物模型是研究少弱精子症发生机制和开发治疗药物不可或缺的重要途径，该文通过对比不同动物模型造模方法，以期为构建更加规范化的少弱精子症动物模型提供思路。目前少弱精子症动物模型有多种造模方法，该文结合近年少弱精子症动物实验文章，选取腺嘌呤造模、奥硝唑造模、雷公藤多苷造模、氢化可的松造模、环磷酰胺造模、白消安造模、紫杉醇造模、热应激造模、电离辐射造模、高脂饮食造模、基因敲除造模等造模方法，从不同造模方法的时间、剂量、动物品系及模型评价标准等方面进行对比，分析当前少弱精子症动物模型的优缺点，发现现有的少弱精子症动物模型仍然存在诸多不足需要进一步改进，动物模型的选择、规范化、创新性等问题都亟待解决，且动物模型与临床患者中医证候的吻合度欠佳。针对目前存在问题，应进一步探索如何构建符合临床特点和证型的造模方法，选用中西医结合复合模型方法，复制更贴近疾病发展规律和符合中医证候的模型，为探索疾病发生机制、开发特色药物和指导临床用药提供动物实验支持。

Abstract

Oligozoospermia and asthenospermia are common causes of clinical male infertility. The number of patients increases year by year and shows a younger trend. Its etiology is complex， the mechanism and unclear pathogenesis and rely on empirical therapy. Therefore， it is necessary for exploring the pathogenesis and developing corresponding drugs to establish reasonable animal models. By comparing different animal model making methods， this paper provides ideas for constructing a more standardized animal model of oligoasthenospermia. At the moment， a lot of molding methods for oligoasthenospermia are available. Combined with the animal experimental articles of oligoasthenospermia in recent years， this study described the modeling with adenine， ornidazole， tripterygium glycoside， hydrocortisone， cyclophosphamide， busulfan， paclitaxel， heat stress， ionizing radiation， high-fat diet， and gene knockout， respectively， and compared the modeling methods in terms of the time， indexes， animal line， and model evaluation. Thereby， the advantages and disadvantages of different models of oligoasthenospermia were summarized， and finds that the existing animal models of oligoasthenospermia still have many shortcomings that need to be further improved. The selection， standardization and innovation of animal models need to be solved urgently， and the coincidence between animal models and clinical patients' traditional Chinese medicine syndromes is not coincident. In view of the existing problems， we should further explore how to build a modeling method in line with clinical characteristics and syndrome types， select the compound model method of integrated traditional Chinese and Western medicine， copy the model closer to the law of disease development and in line with traditional Chinese medicine syndrome， and provide animal experimental support for exploring the mechanism of disease， developing characteristic drugs and guiding clinical medication.

关键词

少弱精子症男性不育造模方法动物模型模型评价

Keywords

oligoasthenospermiamale infertilitymodeling methodanimal modelmodel evaluation

references

何清湖，秦国政.中西医结合男科学［M］.北京：人民卫生出版社，2005：269-274.

世界卫生组织．世界卫生组织人类精液检查与处理实验室手册［M］．5版.谷翊群，陈振文，卢文红，等，译.北京：人民卫生出版社，2011：120.

INHORN M C，PATRIZIO P.Infertility around the globe：New thinking on gender，reproductive technologies and global movements in the 21st century［J］.Hum Reprod Update，2015，21（4）：411-426.

VOCKEL M，RIERA-ESCAMILLA A，TÜTTELMANN F，et al.The X chromosome and male infertility［J］.Hum Genet，2021，140（1）：203-215.

秦素，陈俊，涂定英，等.2018年我院男科门诊病人构成分析［C］// 中国中西医结合学会男科专业委员会.首届男性大健康中西医协同创新论坛暨第三届全国中西医结合男科青年学术论坛论文集：2019年卷.南昌：出版者不详.2019：357-358.

宋春生，赵家有.《EAU男性不育症指南（2012年版）》解读［J］.中国性科学，2012，21（10）：13-16，23.

张志杰，陈小均，贾玉森，等.中医对少弱精子症的临床治疗研究进展［J］.中国性科学，2015，24（9）：71-74.

陈央娣，孙自学.中医药治疗男性不育症的实验研究述评［J］.中华中医药学刊，2022，40（4）：28-33.

陈小均，王思师，贾玉森，等.男性不育症实验动物模型在中医药领域的应用概况［J］.北京中医药，2018，37（3）：282-287.

孔琪，夏霞宇，秦川.实验动物品系数据库的建立［J］.中国比较医学杂志，2015，25（4）：78-83.

鞠成国，李媛媛，王巍，等.仙茅不同炮制品对腺嘌呤致肾阳虚大鼠的作用机制分析［J］.中国实验方剂学杂志，2020，26（16）：101-107.

胡海林，刘子毓，何清湖，等.龟鹿二仙膏对阳虚型少弱精子症大鼠的改善作用及CFTR蛋白表达的影响［J］.中医药信息，2021，38（6）：15-20.

萧闵，王威，曹继刚，等.疏肝补肾毓麟汤调节VDAC2基因甲基化影响弱精症大鼠精子线粒体功能改善精子质量［J］.中国实验方剂学杂志，2021，27（23）：72-79.

徐珂，黄学宽，沈清，等.复肾功方对慢性肾衰竭大鼠ACE-AngⅡ-AT1R及ACE2-Ang（1-7）-MASR轴“调控-拮抗”作用的影响［J］.中国实验方剂学杂志，2021，27（5）：62-69.

BONE W，YEUNG C H，SKUPIN R，et al.Toxicity of ornidazole and its analogues to rat spermatozoa as reflected in motility parameters［J］.Int J Androl，1997，20（6）：347-355.

OBERLÄNDER G，YEUNG C H，COOPER T G.Influence of oral administration of ornidazole on capacitation and the activity of some glycolytic enzymes of rat spermatozoa［J］.J Reprod Fertil，1996，106（2）：231-239.

SUN Y，SUN X，ZHAO L，et al.DJ-1 deficiency causes metabolic abnormality in ornidazole-induced asthenozoospermia［J］.Reproduction，2020，160（6）：931-941.

张稳，刘清珍，商学军，等.L-肉碱对奥硝唑所致弱精子症大鼠的治疗作用［J］.中华男科学杂志，2009，15（7）：604-607.

何康婧，高增平，尹丽梅，等.雷公藤多苷的药理毒理作用研究进展［J］.中国实验方剂学杂志，2020，26（1）：196-204.

徐颖，樊媛芳，赵元，等.近40年雷公藤生殖毒性研究概述［J］.中国中药杂志，2019，44（16）：3406-3414.

刘红娟，吴德玲，童小慧，等.五子衍宗丸干预线粒体通透性转换孔抑制精子凋亡的机制［J］.中国实验方剂学杂志，2020，26（21）：34-39.

JIAO W，SUN J，ZHANG X，et al.Improvement of Qilin pills on male reproductive function in tripterygium glycoside-induced oligoasthenospermia in rats［J］.Andrologia，2021，53（4）：e13923.

QIAN S Z，ZHONG C Q，XU Y.Effect of Tripterigium wilfordii Hook.f.on the fertility of rats［J］.Contraception，1986，33（2）：105-110.

戴冰，张嘉妮，杨梦琳，等.氢化可的松致肾虚证小鼠模型的建立及相关指标的评价［J］.中国实验动物学报，2017，25（1）：70-73.

陈昌波，马静，贾瑊，等.肾阳虚与肾阴虚模型小鼠睾丸生殖相关差异蛋白的比较研究［J］.中华男科学杂志，2019，25（3）：248-256.

GUO Y，WANG L，LI Q，et al.Enhancement of kidney invigorating function in mouse model by cistanches herba dried rapidly at a medium high temperature［J］.J Med Food，2019，22（12）：1246-1253.

BROWN J S，SUNDAR R，LOPEZ J.Combining DNA damaging therapeutics with immunotherapy：More haste，less speed［J］.Br J Cancer，2018，118（3）：312-324.

DONG P，XIA L，HU L，et al.Runjing decoction alleviated cyclophosphamide-induced oligoasthenospermia rats by inhibiting cell apoptosis via RXFP1/AKT/FOXO1 pathway［J］.Andrologia，2021，53（11）：e14216.

REZAEI S，HOSSEINIMEHR S J，ZARGARI M，et al.Protective effects of sinapic acid against cyclophosphamide-induced testicular toxicity via inhibiting oxidative stress，Caspase-3 and NF-κB activity in BALB/c mice［J］.Andrologia，2021，53（10）：e14196.

胡语杰，徐砚通，高健，等.益肾生精方治疗少弱精子症的药效评价及作用机制［J］.中国实验方剂学杂志，2022，28（11）：93-101.

DEHGHANI F，SOTOUDE N，BORDBAR H，et al.The use of platelet-rich plasma （PRP） to improve structural impairment of rat testis induced by busulfan［J］.Platelets，2019，30（4）：513-520.

ZHANG K，FU L，AN Q，et al.Effects of Qilin pills on spermatogenesis，reproductive hormones，oxidative stress，and the TSSK2 gene in a rat model of oligoasthenospermia［J］.BMC Complement Med Ther，2020，20（1）：42.

ABD-ELRAZEK A M，EL-DASH H A，SAID N I.The role of propolis against paclitaxel-induced oligospermia，sperm abnormality，oxidative stress and DNA damage in testes of male rats［J］.Andrologia，2020，52（1）：e13394.

ZICKRI M B，MOUSTAFA M H，FASSEH A E，et al.Antioxidant and antiapoptotic paracrine effects of mesenchymal stem cells on spermatogenic arrest in oligospermia rat model［J］.Ann Anat，2021，237：151750.

李学银，张卫星，张天标，等.两种方式注射白消安对SD大鼠生精功能的影响［J］.郑州大学学报：医学版，2015，50（5）：665-668.

MOGHADAM M T，DADFAR R，KHORSANDI L.The effects of ozone and melatonin on busulfan-induced testicular damage in mice［J］.JBRA Assist Reprod，2021，25（2）：176-184.

MORADI MARYAMNEGHARI S，SHOKRI-ASL V，ABDOLMALEKI A，et al.Genetic，biochemical and histopathological evaluations of thymoquinone on male reproduction system damaged by paclitaxel in Wistar rats［J］.Andrologia，2021，53（10）：e14192.

KHOSRAVI A，HASANI A，BEHNAM P，et al.An effective method for establishing animal models of azoospermia and oligospermia［J］.Andrologia，2021，53（7）：e14095.

ILKHANI S，MORADI A，ALIAGHAEI A，et al.Spatial arrangement of testicular cells disrupted by transient scrotal hyperthermia and subsequent impairment of spermatogenesis［J］.Andrologia，2020，52（9）：e13664.

ROWLEY M J，LEACH D R，WARNER G A，et al.Effect of graded doses of ionizing radiation on the human testis［J］.Radiat Res，1974，59（3）：665-678.

SINGH K V，GAUTAM R，MEENA R，et al.Effect of mobile phone radiation on oxidative stress，inflammatory response，and contextual fear memory in Wistar rat［J］.Environ Sci Pollut Res Int，2020，27（16）：19340-19351.

GAUTAM R，PRIYADARSHINI E，NIRALA J P，et al.Modulatory effects of Punica granatum L juice against 2 115 MHz（3G） radiation-induced reproductive toxicity in male Wistar rat［J］.Environ Sci Pollut Res Int，2021，28（39）：54756-54765.

SAID R S，MOHAMED H A，KASSEM D H.Alpha-lipoic acid effectively attenuates ionizing radiation-mediated testicular dysfunction in rats：Crosstalk of NF-κB，TGF-β，and PPAR-γ pathways［J］.Toxicology，2020，442：152536.

CREAN A J，SENIOR A M.High-fat diets reduce male reproductive success in animal models：A systematic review and Meta-analysis［J］.Obes Rev，2019，20（6）：921-933.

KHORRAMI A，GHANBARZADEH S，ZIAEE M，et al.Dietary cholesterol and oxidised cholesterol：effects on sperm characteristics，antioxidant status and hormonal profile in rats［J］.Andrologia，2015，47（3）：310-317.

LIU C Y，CHANG T C，LIN S H，et al.Metformin ameliorates testicular function and spermatogenesis in male mice with high-fat and high-cholesterol diet-induced obesity［J］.Nutrients，2020，12（7）：1932.

ZHU F，YAN P，ZHANG J，et al.Deficiency of TPPP2，a factor linked to oligoasthenozoospermia，causes subfertility in male mice［J］.J Cell Mol Med，2019，23（4）：2583-2594.

DEVLIN D J，NOZAWA K，IKAWA M，et al.Knockout of family with sequence similarity 170 member A （Fam170a） causes male subfertility，while Fam170b is dispensable in mice†［J］.Biol Reprod，2020，103（2）：205-222.

LIN C Y，CHEN C Y，YU C H，et al.Human X-linked intellectual disability factor CUL4B is required for post-meiotic sperm development and male fertility［J］.Sci Rep，2016，6：20227.

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