Effect of Wenshen Zhuyang Tiaozhou Method on Sex Hormones, Insulin and Ovulation in PCOS Patients with Kidney-Yang Deficiency Syndrome Based on PI3K/Akt Signaling Pathway

Feng-ying LI; Ting-ting ZHAI; Hua YANG; Qi WANG

doi:10.13422/j.cnki.syfjx.20192023

您当前的位置：

首页 >

文章列表页 >

Effect of Wenshen Zhuyang Tiaozhou Method on Sex Hormones, Insulin and Ovulation in PCOS Patients with Kidney-Yang Deficiency Syndrome Based on PI3K/Akt Signaling Pathway

Clinic | 更新时间：2021-02-09

- Effect of Wenshen Zhuyang Tiaozhou Method on Sex Hormones, Insulin and Ovulation in PCOS Patients with Kidney-Yang Deficiency Syndrome Based on PI3K/Akt Signaling Pathway
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 3, Pages: 84-89(2020)
- 作者机构：
  
  贵州中医药大学　第一附属医院，贵阳　 550001
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192023
  CLC： R22; R242; R2-031; R287
- Published：05 February 2020，
  
  Published Online：27 June 2019，
  
  Received：11 February 2019，
- 稿件说明：
扫描看全文
Feng-ying LI, Ting-ting ZHAI, Hua YANG, et al. Effect of Wenshen Zhuyang Tiaozhou Method on Sex Hormones, Insulin and Ovulation in PCOS Patients with Kidney-Yang Deficiency Syndrome Based on PI3K/Akt Signaling Pathway. [J]. Chinese Journal of Experimental Traditional Medical Formulae 26(3):84-89(2020)
DOI：

Feng-ying LI, Ting-ting ZHAI, Hua YANG, et al. Effect of Wenshen Zhuyang Tiaozhou Method on Sex Hormones, Insulin and Ovulation in PCOS Patients with Kidney-Yang Deficiency Syndrome Based on PI3K/Akt Signaling Pathway. [J]. Chinese Journal of Experimental Traditional Medical Formulae 26(3):84-89(2020) DOI： 10.13422/j.cnki.syfjx.20192023.

摘要

目的：

探讨肾阳虚证多囊卵巢综合征(PCOS)患者子宫内膜组织中胰岛素的磷脂酰肌醇-3激酶(PI3K)/蛋白激酶B(Akt)信号通路中Akt的表达及其活化程度，并分析温肾助阳调周法对PCOS患者性激素、胰岛素及排卵的影响。

方法：

选取贵州中医药大学第一附属医院2017年2月至2018年7月收治的60例肾阳虚证PCOS患者，按随机数字表法分为两组，各30例。对照组采取口服炔雌醇环丙孕酮片治疗，观察组在对照组的基础上给予温肾助阳调周法治疗，两组均治疗3个月后随访。测定所有患者的性激素水平、空腹血糖及胰岛素水平，采用稳态模型评估法计算的胰岛素抵抗指数(HOMA-IR)，运用蛋白免疫印迹法检测子宫内膜组织中Akt，p-Akt蛋白的表达水平。观察两组治疗前后性激素、胰岛素及排卵的情况。

结果：

与本组治疗前比较，治疗后两组卵泡刺激素(FSH)和黄体生成素(LH)水平均下降，雌二醇(E

)性激素水平上升(

0.05)，观察组治疗后FSH，LH水平均低于对照组，E

性激素水平高于对照组(

0.05)；与本组治疗前比较，治疗后两组FPG，FINS均明显降低(

0.05)，观察组治疗后FPG，FINS水平明显低于对照组(

0.05)；与本组治疗前比较，治疗后两组患者的HOMA-IR及血清脂联素均有明显下降(

0.05)，观察组治疗后HOMA-IR及血清脂联素明显低于对照组(

0.05)；与本组治疗前比较，治疗后两组卵巢体积、窦卵细胞数目均明显降低，子宫内膜厚度增加(

0.05)，观察组治疗后卵巢体积、窦卵细胞数目明显低于对照组，子宫内膜厚度明显大于对照组(

0.05)；与本组治疗前比较，治疗后观察组与对照组子宫内膜Akt和p-Akt蛋白明显降低，观察组治疗后子宫内膜Akt和p-Akt明显低于对照组(

0.05)。

结论：

采用温肾助阳调周法可有效的改善肾阳虚证PCOS患者性激素水平，调控胰岛素，促进卵巢功能恢复，这可能与阻断Akt磷酸化，抑制PI3K/Akt信号通路活化有关。

Abstract

Objective:

To investigate the effect of Wenshen Zhuyang Tiaozhou method on sex hormones

insulin and ovulation in polycystic ovary syndrome (PCOS) patients with kidney-Yang deficiency syndrome based on phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway.

Method:

Totally 60 PCOS patients with kidney-Yang deficiency syndrome treated at the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine from February 2017 to July 2018 were selected and divided into observation group and control group according to the random number table method

with 30 cases in each group. The control group was orally treated with ethinylestradiol and cycloproterone acetate tablets

in addition to this therapy

the observation group also received Wenshen Zhuyang Tiaozhou method. Both groups were followed up after three months of treatment. Sex hormone levels

fasting plasma glucose (FPG) and fasting insulin (FINS) levels were measured in all patients. Homeostasis model assessment-insulin resistance(HOMA-IR) was calculated by homeostasis model assessment method

and the expression levels of Akt and p-Akt proteins in endometrium were detected by Western blot.

Result:

After treatment

the levels of FSH and LH in both groups decreased

and the level of E

sex hormone increased (

0.05). After treatment

the levels of FSH and LH in observation group were lower than those in control group

whereas the level of E

sex hormone was higher than that in control group (

0.05). After treatment

the levels of FPG and FINS in two groups were significantly lower (

0.05)

while the levels of FPG and FINS in observation group were significantly lower than those in control group (

0.05). After treatment

the ovarian volume and the number of sinusoidal oocytes in two groups decreased significantly

while the endometrial thickness increased (

0.05). After treatment

the ovarian volume and the number of sinusoidal oocytes in observation group were significantly lower than those in control group

and the endometrial thickness was significantly lower than that in observation group (

0.05). After treatment

the ovarian volume and the number of sinusoidal oocytes in observation group were significantly lower than those in control group

and the endometrial thickness was larger than that of control group (

0.05). After treatment

the Akt and p-Akt proteins in the endometrium of observation group and control group were significantly decreased

while the Akt and p-Akt proteins in the endometrium of observation group were significantly lower than those of control group (

0.05).

Conclusion:

Wenshen Zhuyang Tiaozhou method can effectively ameliorate the sex hormone level

regulate insulin and promote the recovery of ovarian function in PCOS patients with kidney-Yang deficiency syndrome. This may be related to the blocking of the phosphorylation of Akt and the inhibition of the activation of PI3K/Akt signaling pathway.

关键词

磷脂酰肌醇-3激酶蛋白激酶B肾阳虚证多囊卵巢综合征性激素胰岛素

Keywords

phosphatidylinositol 3-kinase (PI3K)protein kinase B (Akt)kidney-Yang deficiency syndromepolycystic ovary syndrome (PCOS)sex hormonesinsulin

references

邵译萱．周惠芳．运用补肾调周法治疗痰湿型多囊卵巢综合征不孕经验[J]．安徽中医药大学学报，2017，36(6)：41-43.

张琳，吴迎，曾凡星，等．PI3K/Akt和MAPK信号通路平衡在运动调控高血压血管平滑肌表型转换中的作用[J]．中国体育科技，2017，53(6)：108-116.

凡治国，李志平，任超. PI3K/Akt信号通路阻断药联合小檗碱对前列腺癌DU145细胞生长的抑制作用[J]．癌症进展，2018，16(5): 563-566，579.

李洪波，王云贡，姚明，等．骨癌痛大鼠背根神经节IL-17与PI3K/Akt信号通路的关系[J]．中华麻醉学杂志，2016，36(7): 1000-1003，1011.

王黎，柯鸿．淫羊藿苷调控急性淋巴细胞白血病PI3K/Akt信号传导通路的实验研究[J]．中国中医急症，2018，27(6): 1000-1003，1011.

李俊波，邓芳，李霄汉，等. 龙胆泻肝汤对PCOS模型大鼠的影响[J].中国实验方剂学杂志，2018, 24(7): 121-126.

范明明，王建英，韩凤娟，等. 多囊卵巢综合征的中医探究[J].世界中西医结合杂志，2009, 4(6)：446-448.

李凤英，王琪. 王琪教授治疗多囊卵巢综合征所致不孕经验[J].贵阳中医学院学报，2010, 32(6)：13-14.

王琪，李凤英. 应用暖宫助孕汤结合克罗米芬治疗肾阳虚型多囊卵巢综合征的疗效观察[J].贵阳中医学院学报，2013, 35(6): 33-34.

王琪，李凤英. 暖宫助孕汤结合克罗米芬对多囊卵巢综合征(肾阳虚型)卵泡发育的影响[J]．辽宁中医杂志，2012, 39(9)：1752-1754.

Rotterdam ESHRE/ASRM-Sponsored PCOS consensus workshop group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome(PCOS)[J].Hum Reprod, 2004, 19(1)：41-47.

中华人民共和国卫生部．中药新药临床研究指导原则[M]．北京：中国医药科技出版社，2002：413-415.

张玉珍．中医妇科学[M].7版．北京：中国中医药出版社，2007：233-235.

谢盈彧，许晓敏，张军平，等．基于PI3K/Akt/mTOR通路探讨补肾抗衰片介导自噬调控动脉粥样硬化的机制[J]．中国中西医结合杂志，2018，38(5): 586-593.

郭勇英，位庚，李红蓉，等．通心络联合外周血间充质干细胞移植对糖尿病足大鼠血管新生磷脂酰肌醇3-激酶/蛋白激酶B信号通路的影响研究[J]．中国全科医学，2016, 19(13)：1602-1606.

黄小波，王宁群，陈玉静，等．陈皮半夏通过PI3K-Akt通路对衰老脐静脉内皮细胞中p53和p21表达的影响[J]．北京中医药，2017, 36(9)：796-799.

Y Tsujita，K Mitsui-Sekinaka，K Imai，et al.Phosphatase and tensin homolog (PTEN) mutation can cause activated phosphatidylinositol 3-kinase δ syndrome-like immunodeficiency[J]．J Allergy Clin Immunol，2016，16(7)：163-165.

戴冰，吴沁璇，肖子曾，等．六味地黄汤及其水提醇溶部位对2型糖尿病模型大鼠脂肪组织中PI3K/Akt信号通路的影响[J]．中成药，2016，38(2)：428-430.

叶元梅，冷玉芳，杨佩宁，等．羟考酮预先给药对大鼠心肌缺血再灌注损伤的影响及其与PI3K/Akt信号通路的关系[J]．中华麻醉学杂志，2016，36(8)：946-948.

赵恒侠，周道成，李惠林，等．荷芪散对多囊卵巢综合征大鼠内分泌代谢及PI3K/Akt信号通路的影响[J]．现代中西医结合杂志，2018，27(2)：130-135.

饶红，武福云，陈德森．淫羊藿素对BALB/c-nu裸鼠前列腺癌组织磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)信号通路及E-钙黏蛋白的影响[J]．中国中医急症，2018，27(5): 789-792，796.

张涛，马媛萍，黄晓燕，等．健脾清热活血方干预PI3K-Akt/mTOR信号通路调控CDK1表达防治溃疡性结肠炎相关癌变[J]．辽宁中医杂志，2017，44(6): 1124-1128，1115.

洪金妮，黎巍威，张宁，等．中药降糖复方水提物通过PI3K/Akt/NF-κB信号通路抑制KK-Ay小鼠糖尿病肾病炎症[J]．药物评价研究，2017，40(10)：1389-1396.

T E Eichler，B Becknell，R S Easterling，et al.Insulin and the phosphatidylinositol 3-kinase signaling pathway regulate Ribonuclease 7 expression in the human urinary tract[J]．Kidney Int，2016，25(16)：302-304.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect and Mechanism of Nongsuo Dangguiwan in Relieving Oxidative Stress in Premature Ovarian Insufficiency Rats Based on Nrf2/HO-1 Signaling Pathway

Efficacy and Mechanism of Yishen Shengjing Prescription in Treatment of Oligoasthenospermia

Clinical Observation of Modified Buzhong Yiqitang Combined with Erxian Decoction in Treatment of Stress Urinary Incontinence in Perimenopausal Women

Modified Qiaohetang and Xiehuangsan in Treatment of Acne Due to Dampness-heat Accumulation

Clinical Efficacy on Yishen Tongluofang for Treatment of Male Infertility with Deficiency and Stasis of Kidney

Related Author

LUO Jiaqi

DU Xiaoli

CHEN Dongmei

CHEN Miao

HU Na

XING Shasha

LIU Wenzhao

TIAN Ruiying

Related Institution

Ningxia Medical University

Laboratory Animal Centre，Ningxia Medical University

Key Laboratory of Fertility Presevation and Maintenance（NXMU），Ministry of Education， Ningxia Medical University

Key Laboratory of Ningxia Minority Medicine Modernization Ministry of Education，Ningxia Medical University

Ningxia Regional Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Regional High Incidence Disease

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰