Material Basis Analysis of Toxicity Attenuation and Efficacy Reservation of Processing of <italic style="font-style: italic">Gelsemium elegans</italic> Rhizomes

Li REN; Gao-pan LI; Sai-nan CHEN; Wen XU; He-shan WANG; Mei-xia HUANG; Hua-jun LIAO; Shui-sheng WU

doi:10.13422/j.cnki.syfjx.20191948

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Material Basis Analysis of Toxicity Attenuation and Efficacy Reservation of Processing of Gelsemium elegans Rhizomes

Pharmacy and Processing | 更新时间：2021-02-09

- Material Basis Analysis of Toxicity Attenuation and Efficacy Reservation of Processing of Gelsemium elegans Rhizomes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 26, Issue 3, Pages: 117-124(2020)
- 作者机构：
  
  福建中医药大学　药学院，生物医药研发中心，福州　 350122
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20191948
  CLC： R22; R28; R943.1; O212; R914
- Published：05 February 2020，
  
  Published Online：19 June 2019，
  
  Received：08 May 2019，
- 稿件说明：
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Li REN, Gao-pan LI, Sai-nan CHEN, et al. Material Basis Analysis of Toxicity Attenuation and Efficacy Reservation of Processing of Gelsemium elegans Rhizomes. [J]. Chinese Journal of Experimental Traditional Medical Formulae 26(3):117-124(2020)
DOI：

Li REN, Gao-pan LI, Sai-nan CHEN, et al. Material Basis Analysis of Toxicity Attenuation and Efficacy Reservation of Processing of Gelsemium elegans Rhizomes. [J]. Chinese Journal of Experimental Traditional Medical Formulae 26(3):117-124(2020) DOI： 10.13422/j.cnki.syfjx.20191948.

摘要

目的：

在前期研究基础上，检测钩吻炮制前后的6种主要生物碱成分的变化情况，以期揭示钩吻炮制后减毒存效的内在机制。

方法：

采用HPLC同时测定钩吻素甲、胡蔓藤碱丙、钩吻素子、钩吻素己、钩吻绿碱、胡蔓藤碱乙6个成分的含量，色谱条件为流动相甲醇(A)-0.1%甲酸水溶液(B)梯度洗脱(0～10 min，22%A；10～20 min，22%～30%A；20～30 min，30%～40%A)，流速1 mL·min

-1

，检测波长254 nm，柱温30 ℃，进样量10 μL。采用聚类分析和主成分分析法对钩吻炮制前后化学成分变化情况进行分析。

结果：

炮制前上述6种成分在12批生品中的平均质量分数分别为1.444，1.129，3.590，1.603，2.376，1.631 mg·g

-1

；炮制后六者的平均质量分数则依次为2.258，0.343，1.176，0.115，0.459，0.281 mg·g

-1

；毒性最强成分钩吻素己质量分数下降最为显著，下降率达92.83%，而毒性较小的钩吻素甲炮制后质量分数升高了56.37%，其他4个成分质量分数均有不同程度的降低。聚类分析结果表明钩吻炮制前后可明显分为2个类别，主成分分析表明钩吻炮制后第1主成分由钩吻素子变为钩吻素甲。

结论：

钩吻炮制后毒性成分钩吻素己被显著降解，胡蔓藤碱丙、钩吻素子、钩吻绿碱、胡蔓藤碱乙成分含量下降以及钩吻素甲的含量升高可能是钩吻炮制后减毒存效的内在机制之一。

Abstract

Objective:

On the basis of previous research

to detect the changes of six main alkaloids in

Gelsemium elegans

rhizomes before and after being processed

so as to reveal its internal mechanism of processing.

Method:

The contents of gelsemine

humantenidine

koumine

gelsenicine

gelsevirine and humantenine in

elegans

rhizomes was determined simultaneously by HPLC

the content changes of these components before and after processing and its reasons were analyzed by cluster analysis and principal component analysis. The mobile phase was methanol (A)-0.1%formic acid aqueous solution (B) for gradient elution (0-10 min

22%A; 10-20 min

22%-30%A; 20-30 min

30%-40%A). The flow rate was 1.0 mL·min

-1

. The detection wavelength was set at 254 nm

the injection volume was 10 μL

and the column temperature was 30 ℃.

Result:

Before processing

contents of the above six components in raw products were 1.444

1.129

3.590

1.603

2.376

1.631 mg·g

-1

after processing

the contents of these six components were 2.258

0.343

1.176

0.115

0.459

0.281 mg·g

-1

respectively. Gelsenicine

the most toxic ingredient of

elegans

rhizomes

decreased most significantly with a decreasing rate of 92.83%

while the less toxic ingredient

gelsemine

increased by 56.37%after processing. The contents of other four components in

elegans

rhizomes decreased to varying degrees after processing. The results of cluster analysis indicated that

elegans

rhizomes were clearly divided into two categories before and after processing. Principal component analysis showed that the first principal component before and after processing was changed from koumine to gelsemine.

Conclusion:

The degradation of toxic components and content changes of other components may be one of the intrinsic mechanism of toxicity attenuation and efficacy reservation of

elegans

rhizomes being processed.

关键词

钩吻炮制生物碱钩吻素己钩吻素甲聚类分析主成分分析

Keywords

Gelsemium elegans rhizomesprocessingalkaloidsgelsenicinegelseminecluster analysisprincipal component analysis

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Material Basis Analysis of Toxicity Attenuation and Efficacy Reservation of Processing of Gelsemium elegans Rhizomes

DOI：10.13422/j.cnki.syfjx.20191948

摘要

Abstract

关键词

Keywords

references