Analysis on YABBY Transcription Factor Family of Original Plant <italic style="font-style: italic">Cannabis sativa</italic> of Traditional Chinese Medicine Cannabis Fructus

Jia-ying SUN; Zhen WANG; Yao-lei MI; Xiang-xiao MENG; Hui-hua WAN; Yun YAN; Wei SUN; Wei MA

doi:10.13422/j.cnki.syfjx.20202115

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Analysis on YABBY Transcription Factor Family of Original Plant Cannabis sativa of Traditional Chinese Medicine Cannabis Fructus

Pharmacy Fundamentals | 更新时间：2021-02-09

- Analysis on YABBY Transcription Factor Family of Original Plant Cannabis sativa of Traditional Chinese Medicine Cannabis Fructus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 5, Pages: 124-131(2021)
- 作者机构：
  
  1.黑龙江中医药大学药学院，哈尔滨 150040
  2.中国中医科学院中药研究所，北京 100700
  3.贵州百灵企业集团制药股份有限公司，贵州安顺 561000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20202115
  CLC： R284.2;R289;R22;R2-031
- Published：05 March 2021，
  
  Published Online：10 August 2020，
  
  Received：18 May 2020，
- 稿件说明：
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孙嘉莹,王震,米要磊等.中药火麻仁基原植物大麻全基因组YABBY转录因子分析[J].中国实验方剂学杂志,2021,27(05):124-131.

SUN Jia-ying,WANG Zhen,MI Yao-lei,et al.Analysis on YABBY Transcription Factor Family of Original Plant Cannabis sativa of Traditional Chinese Medicine Cannabis Fructus[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(05):124-131.
孙嘉莹,王震,米要磊等.中药火麻仁基原植物大麻全基因组YABBY转录因子分析[J].中国实验方剂学杂志,2021,27(05):124-131. DOI： 10.13422/j.cnki.syfjx.20202115.

SUN Jia-ying,WANG Zhen,MI Yao-lei,et al.Analysis on YABBY Transcription Factor Family of Original Plant Cannabis sativa of Traditional Chinese Medicine Cannabis Fructus[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(05):124-131. DOI： 10.13422/j.cnki.syfjx.20202115.

摘要

目的

分析大麻（

Cannabis sativa

）YABBY转录因子家族序列特征、染色体定位、基因结构、保守基序、系统进化和基因的差异表达；为深入研究

YABBY

基因功能提供分子基础，为工业大麻优良品种选育提供理论支撑。

方法

通过生物信息学手段对火麻仁基原植物大麻

YABBY

基因家族进行鉴定和分析，使用PlantTFDB，ExPASy，MEME，WoLFPSORT，PLANTCARE等在线网站及TBtools，MEGA，DNAMAN等软件进行YABBY预测和分析并进行可视化作图。

结果

大麻中含有6个

YABBY

基因成员，分布在5条染色体上，其中5个定位在细胞核，1个定位在胞外；氨基酸数目185~235个，等电点介于5.05和9.34，相对分子质量在20 582.45~26 282.7 Da；所有CsYABBY蛋白都包含有锌指结构和YABBY两个保守结构域，

CsYABBY

基因启动子区具有多种与胁迫相关的顺式作用元件，并且在不同部位和不同品种的表达量存在差异。

结论

该基因家族的表达可能会受到激素和外界环境因素影响，大麻

YABBY

基因具有组织表达特异性，花中特异性高表达的基因可能对大麻雌花的发育有重要调控作用，YAB1和YAB5亚族的CsYABBY可能参与大麻素的合成过程。

Abstract

Objective

To analyze the sequence characteristics，chromosomal location，gene structure，conserved motifs，phylogenetic evolution and differential gene expressions of the

Cannabis sativa

YABBY transcription factor family，in order to provide a molecular basis for in-depth study of

YABBY

gene function and theoretical support for the selection and breeding of superior hemp varieties.

Method

The bio-informatics method was used to identify and analyze the

CsYABBY

gene family of the original hemp seed plant. PlantTFDB，ExPASy，MEME，CELLO，PLANTCARE and other online websites and TBtools，MEGA，DNAMAN and other software were used for prediction，visualization and analysis.

Result

C. sativa

contains 6

YABBY

gene members distributed on 5 chromosomes，in which 5 members are localized in the nucleus and 1 in extracellular， they consist of 185-235 amino acids， and the isoelectric point is between 5.05 and 9.34， the molecular weight is between 20 582.45-26 282.7 Da. All of CsYABBY proteins contain two conserved domains， namely Zinc finger domain and YABBY domain.

CsYABBY

genes have multiple cis-acting elements，and their expressions differ in different tissues and cultivars.

Conclusion

The expressions of CsYABBY may be affected by hormones and externally environmental factors.

CsYABBY

gene expressions are tissue-specific. In addition，YABBY transcription factor family may play an important role in regulating the development of

C. sativa

female flowers，and subfamilies YAB1 and YAB5 may be involved in the synthesis of cannabinoids.

关键词

生物信息学大麻YABBY基因保守分析进化分析

Keywords

bioinformaticsCannabis sativaYABBY geneconservative analysisevolutionary analysis

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Related Institution

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Analysis on YABBY Transcription Factor Family of Original Plant Cannabis sativa of Traditional Chinese Medicine Cannabis Fructus

DOI：10.13422/j.cnki.syfjx.20202115

摘要

Abstract

关键词

Keywords

references