Effect of Shaoyaotang on Expressions of CD14, FADD and Caspase-8 in Colonic Tissues of Rats with Large Intestinal Damp-heat Syndrome of Ulcerative Colitis

Si-qi CAO; Feng-yi WANG; Sheng-nan TANG; Dang-sheng ZHAO; Yang-yang LI; Zhi-jie LIU; Rui-ting CHAI

doi:10.13422/j.cnki.syfjx.20202401

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Effect of Shaoyaotang on Expressions of CD14, FADD and Caspase-8 in Colonic Tissues of Rats with Large Intestinal Damp-heat Syndrome of Ulcerative Colitis

Classic Prescriptions | 更新时间：2021-02-09

- Effect of Shaoyaotang on Expressions of CD14, FADD and Caspase-8 in Colonic Tissues of Rats with Large Intestinal Damp-heat Syndrome of Ulcerative Colitis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 27, Issue 5, Pages: 1-7(2021)
- 作者机构：
  
  1.甘肃中医药大学，兰州 730000
  2.深圳市宝安纯中医治疗医院，广州深圳 518101
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20202401
  CLC： R2-0;R22;R285.5;R289
- Published：05 March 2021，
  
  Published Online：19 November 2020，
  
  Received：13 October 2020，
- 稿件说明：
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曹思齐,王凤仪,汤胜男等.芍药汤对大肠湿热型溃疡性结肠炎大鼠结肠组织中CD14，FADD，Caspase-8表达的影响[J].中国实验方剂学杂志,2021,27(05):1-7.

CAO Si-qi,WANG Feng-yi,TANG Sheng-nan,et al.Effect of Shaoyaotang on Expressions of CD14, FADD and Caspase-8 in Colonic Tissues of Rats with Large Intestinal Damp-heat Syndrome of Ulcerative Colitis[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(05):1-7.
曹思齐,王凤仪,汤胜男等.芍药汤对大肠湿热型溃疡性结肠炎大鼠结肠组织中CD14，FADD，Caspase-8表达的影响[J].中国实验方剂学杂志,2021,27(05):1-7. DOI： 10.13422/j.cnki.syfjx.20202401.

CAO Si-qi,WANG Feng-yi,TANG Sheng-nan,et al.Effect of Shaoyaotang on Expressions of CD14, FADD and Caspase-8 in Colonic Tissues of Rats with Large Intestinal Damp-heat Syndrome of Ulcerative Colitis[J].Chinese Journal of Experimental Traditional Medical Formulae,2021,27(05):1-7. DOI： 10.13422/j.cnki.syfjx.20202401.

摘要

目的

观察芍药汤对大肠湿热型溃疡性结肠炎（UC）模型大鼠血清中细胞黏附分子-1（ICAM-1），转化生长因子-

（TGF-

）含量及病灶结肠组织中白细胞分化抗原14（CD14），Fas相关死亡结构域蛋白（FADD）与天冬半胱氨酸蛋白酶-8（Caspase-8） mRNA及蛋白表达的影响。

方法

将SPF级Wistar大鼠80只随机分为正常组10只、造模组70只，采用病证结合的方式复制大肠湿热型UC大鼠，即高脂高糖辛辣饮食+2，4-二硝基苯磺酸（DNBS）结合乙醇复合法造模，造模成功后，按随机数字表法将造模组分为模型组、柳氮磺砒啶（SASP）组及芍药汤低、中、高剂量组，每组14只，给予柳氮磺嘧啶0.2 g·kg

-1

·d

-1

，芍药汤低、中、高剂量（6，12，24 g·kg

-1

·d

-1

）灌胃，正常组及模型组给予等体积生理盐水灌胃，连续21 d。采用酶联免疫吸附测定（ELISA）法检测血清ICAM-1，TGF-

含量，实时荧光定量聚合酶链式反应（Real-time PCR）检测结肠组织CD14，FADD，Caspase-8 mRNA表达，蛋白免疫印迹法（Western blot）检测结肠组织CD14，FADD，Caspase-8蛋白表达。

结果

与正常组比较，模型组大鼠血清中ICAM-1含量明显升高，TGF-

含量明显降低（

0.05）；CD14，FADD，Caspase-8 mRNA及蛋白相对表达量明显升高（

0.05）。与模型组比较，芍药汤中、高剂量组及SASP组大鼠血清中ICAM-1含量明显降低，而芍药汤低、中、高剂量组及SASP组大鼠血清中TGF-

含量明显升高（

0.05）；各干预组CD14，FADD，Caspase-8 mRNA及蛋白表达量明显降低（

0.05），并以芍药汤高剂量组及SASP组更为明显。

结论

芍药汤对大肠湿热型UC大鼠具有一定干预作用，其机制可能与抑制CD14，FADD及Caspase-8 mRNA及蛋白的表达有关。

Abstract

Objective

To observe the effect of Shaoyaotang on the contents of cell adhesion molecule-1 （ICAM-1） and transforming growth factor-

（TGF-

） in serum of large intestine damp-heat syndrome of ulcerative colitis （UC） in rats， and the gene and protein expressions of leukocyte differentiation antigen14 （CD14）， Fas-related death domain protein （FADD） and cysteinyl aspartate specific protease-8 （Caspase-8） in the focal colon tissue.

Method

A total of 80 SPF Wistar rats were randomly divided into the blank group （

=10） and modeling group （

=70）. The large intestine damp-heat syndrome of UC rats was replicated by the combination of disease and syndrome， which was high-fat， high-sugar and spicy diets combined with 2， 4-dinitrobenzene sulfonic acid （DNBS） and ethanol. After successful modeling， the modeled groups were divided into model group， sulfasalazine （SASP）control group， and low， medium and high-dose Shaoyaotang groups by the method of random number table， with14 rats in each group. Low， medium and high doses of Sulfasalazine 0.2 g·kg

-1

·d

-1

and Shaoyaotang （6， 12， 24 g·kg

-1

·d

-1

）were given by gavage. The blank group and the model group were given equal volume of normal saline for 21 days. The contents of serum ICAM-1 and TGF-

were detected by enzyme-linked immunosorbent assay （ELISA）， the expressions of CD14， FADD and Caspase-8 mRNA in colon tissues were detected by Real-time quantitative polymerase chain reaction （Real-time PCR）， and the expressions of CD14， FADD and Caspase-8 protein in colon tissues were detected by Western blot.

Result

Compared with the blank group， the serum ICAM-1 level in the model group were significantly increased， whereas the content of TGF-

were significantly decreased （

0.05）. The relative expression levels of CD14， FADD， Caspase-8 mRNA and protein were significantly increased （

0.05）. Compared with the model group， the content of ICAM-1 in the serum of the rats in the medium， high-dose Shaoyaotang groups and the SASP group were significantly decreased， while the content of TGF-

in the serum of the rats in the low， medium， high-dose Shaoyaotang groups and the SASP group were significantly increased （

0.05）. The expression levels of CD14， FADD， Caspase-8 mRNA and protein in each intervention group were significantly decreased （

0.05）， especially in the high-dose Shaoyaotang group and the SASP group.

Conclusion

Shaoyaotang has a certain intervention effect on UC rats with large intestine damp-heat syndrome， and its mechanism may be related to the inhibition of CD14， FADD and Caspase-8 genes and proteins expression.

关键词

芍药汤溃疡性结肠炎白细胞分化抗原14（CD14）Fas相关死亡结构域蛋白（FADD）天冬半胱氨酸蛋白酶-8(Caspase-8)

Keywords

Shaoyaotangulcerative colitisleukocyte differentiation antigen 14 （CD14）Fas-related death domain protein （FADD）cysteinyl aspartate specific protease-8 （Caspase-8）

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