Screening and Verification of Compatibility Proportion of Effective Components from Traditional Chinese Medicine of Diabetes

Xiao WANG; Xiao-bo QU; Bo LIU; Xin WANG; Lian-lian SONG; Hui-bo XU

doi:10.13422/j.cnki.syfjx.20192136

您当前的位置：

首页 >

文章列表页 >

Screening and Verification of Compatibility Proportion of Effective Components from Traditional Chinese Medicine of Diabetes

Pharmacology | 更新时间：2021-02-09

- Screening and Verification of Compatibility Proportion of Effective Components from Traditional Chinese Medicine of Diabetes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 21, Pages: 45-52(2019)
- 作者机构：
  
  1.长春中医药大学　药学院，长春　 130117；
  2.吉林省中医药科学院，长春　 130012
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192136
  CLC： R2-0; R285; R587.1
- Published：05 November 2019，
  
  Published Online：18 July 2019，
  
  Received：09 May 2019，
- 稿件说明：
扫描看全文
Xiao WANG, Xiao-bo QU, Bo LIU, et al. Screening and Verification of Compatibility Proportion of Effective Components from Traditional Chinese Medicine of Diabetes. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(21):45-52(2019)
DOI：

Xiao WANG, Xiao-bo QU, Bo LIU, et al. Screening and Verification of Compatibility Proportion of Effective Components from Traditional Chinese Medicine of Diabetes. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(21):45-52(2019) DOI： 10.13422/j.cnki.syfjx.20192136.

摘要

目的：

运用均匀设计结合药理模型的方法筛选黄连总生物碱与三七总皂苷组合物(HS组合物)的最佳配伍比例，观察该剂量HS组合物对糖尿病并发症的影响。

方法：

以黄连总生物碱、三七总皂苷为研究对象，根据均匀设计方案选用U

)表分组设计，以小鼠血糖、血浆凝血酶原时间(PT)，活化部分凝血酶原时间(APTT)为筛选指标，回归分析获得最佳剂量配比。采用高糖高脂结合链脲佐菌素诱导2型糖尿病大鼠模型，将大鼠分为空白组，模型组，二甲双胍组(150 mg·kg

－1

)，HS组合物组(黄连总生物碱360 mg·kg

－1

，三七总皂苷40 mg·kg

－1

)，灌胃给药10周，通过观察各组大鼠血糖、糖耐量并计算血糖曲线下面积(AUC)，甘油三酯(TG)，高密度脂蛋白胆固醇(HDL-C)与低密度脂蛋白胆固醇(LDL-C)，血液流变学指标以及胰腺、心脏、肾脏、视网膜的病理变化，验证该组合物对糖尿病并发症的作用。

结果：

HS组合物的小鼠给药最佳配伍比例为黄连总生物碱625 mg·kg

－1

＋三七总皂苷60 mg·kg

－1

。验证实验显示，与空白组比较，模型组小鼠空腹血糖及血脂水平显著升高(

0.01)，HDL-C显著降低(

0.01)；全血高、中、低切黏度均显著升高(

0.01)，胰腺、心脏、肾脏、视网膜损伤程度较重；与模型组比较，二甲双胍组于给药第6，8，10周血糖明显降低(

0.05，

0.01)，HS组合物组第2，4，8，10周空腹血糖水平明显降低(

0.05，

0.01)，血清中TG及LDL-C值明显降低(

0.05，

0.01)，HDL-C，HDL-C/LDL-C值显著升高(

0.01)，全血高切黏度与全血中切黏度均显著降低(

0.01)；病理结果显示，HS组合物能减轻大鼠胰腺、心脏、肾脏以及视网膜的损伤。

结论：

HS组合物最佳配伍剂量对高糖高脂结合链脲佐菌素诱导的大鼠糖尿病并发症具有良好的抑制作用。

Abstract

Objective:

To screen the best compatibility proportion of total alkaloids of Coptidis Rhizoma and Notoginseng Radix et Rhizoma total saponins (HS composition)by uniform design and pharmacological model and to observe the effect on diabetic complications.

Method:

The total alkaloids of Coptidis Rhizoma and Notoginseng Radix et Rhizoma total saponins were used as the research objects

) table was choosed for grouping design.The content of triglyceride fasting blood-glucose (FBG)

prothrombin time (PT) and active partial thromboplastin time (APTT) was chosen as index. The best dose ratio was obtained by multipleregression analysis. Rats with type 2 diabetes mellitus induced by high-fat diet combined streptozotocin were divided into blank group

model group

metformin group (150 mg·kg

-1

)

HS composition group (total alkaloids of Coptidis Rhizoma 360 mg·kg

-1

+ Notoginseng Radix et Rhizoma total saponins 40 mg·kg

-1

). Rats were administered orally for 10 weeks.By observing the blood glucose

glucose tolerance

area under the curve (AUC)

triglyceride (TG)

high-density lipoprotein (HDL-C)and low-density lipoprotein (LDL-C)

hemorheological indexes and pathological changes of pancreas

heart

kidney and retina in rats of each group

the effect of this composition on diabetic complications was verified.

Result:

Combination of 625 mg·kg

-1

total alkaloids of Coptidis Rhizoma and 60 mg·kg

-1

Notoginseng Radix et Rhizoma total saponins was the optimal dosage ratio of HS composition.The validation test showed that compared with blank group

the fasting blood glucose and lipid levels in the model group were significantly increased (

0.01)

and HDL-C was decreased (

0.01). The hemorheology indexes of whole blood were significantly increased (

0.01). Compared with model group

the FBG in metformin group were significantly reduced at week 6

10(

0.05

0.01). Compared with model group

HS combination group 2

10 weeks significantly lower fasting glucose levels(

0.05

0.01)

serum TG

LDL-C significantly lower values(

0.05

0.01)

HDL-C

HDL-C/LDL-C values were significantly increased (

0.01)

whole blood high shear viscosity and whole blood viscosity were significantly reduced (

0.01). HS composition could reduce the blood sugar and lipid content

reduce the whole blood viscosity

and reduce the damage of pancreas

heart

kidney and retina.

Conclusion:

The optimum compatibility dose of HS composition have a good therapeutic effect on diabetic complication rats induced by high-fat diet and streptozotocin.

关键词

糖尿病并发症黄连总生物碱三七总皂苷均匀设计配伍比例

Keywords

diabetic complicationtotal alkaloids of Coptidis RhizomaNotoginseng Radix et Rhizoma total saponinsuniform designcompatibility proportion

references

刘彤彤，肖琴，盛军庆，等．基于nano-LC-LTQ/Orbitrap-MS筛选黄芩-黄连药对干预2型糖尿病KKAy小鼠的骨骼肌差异表达蛋白[J]．中国实验方剂学杂志，2018，24(17)：126-131．

陈一君，吴庆光，刘颖，等．黄连解毒汤单独用药及联合用药对2型糖尿病治疗效果的Meta分析[J]．中国实验方剂学杂志，2018，24(10)：212-220．

翟若男，陈腾，简桂花，等．益气活血方防治糖尿病肾病足细胞损伤的实验研究[J]．中华中医药学刊，2019，37(3)：583-585，776．

黄泽禹，陈娟，陈清杰．小檗碱调控线粒体功能改善2型糖尿病引起的肝脏脂质代谢紊乱[J]．华中科技大学学报：医学版，2019，48(1)：53-57．

黄娟，胡维，林湘东．盐酸小檗碱对2型糖尿病胰岛素抵抗的改善作用及其机制[J]．中国老年学杂志，2018，38(17)：4130-4132．

崔祥，陶金华，江曙，等．黄连提取物与肠道菌群的相互作用研究[J]．中草药，2018，49(9)：2103-2107．

YAN Q F, TAN P Z, LIU Q T, et al. Influence of Panax Notoginseng Saponins on serums ICAM-1 in patients with acute cerebral infarction [J].Chin J Pract Nerv Dis, 2008, 2(1): 56-57.

SHI Q H, XIANG J, ZHU X Y, et al. Protective effects of Chinese herbal medicine Naoshuantong on neurovascular unit in rats with cerebral ischemia/reperfusion injury [J].J Chin Integr Med, 2012, 10(10): 1135-1139.

贾卓雅，石凯行，范彦芳，等．丹皮酚联合三七总皂苷对大鼠糖尿病心肌纤维化的影响[J]．中国实验方剂学杂志，2018，24(6)：133-138．

乔园，段惠惠，黄建梅，等．三七总皂苷对高糖诱导大鼠视网膜微血管内皮细胞损伤的保护作用[J]．北京中医药大学学报，2015，38(5)：318-322，361．

杨文英．中国糖尿病的流行特点及变化趋势[J]．中国科学：生命科学，2018，48(8)：812-819．

Memon M A, Khan R N, Riaz S, et al. Methylglyoxal and insulin resistance in berberine-trated type 2 diabetic patients [J].J Res Med Sci, 2018, 23: 110.

MA X, CHEN Z, WANG L, et al. The Pathogenesis of diabetes mellitus by oxidative stress and inflammation: its inhibition by berberine [J].Front Pharmacol, 2018, 9: 782.

田硕，吴丽丽，张卫华，等．基于AMPK/ACC信号通路探讨夏枯草提取物调节ZDF大鼠脂代谢的机制[J]．中国实验方剂学杂志，2019，25(9)：82-88．

Paul M, Hemshekhar M, Kemparaju K, et al. Berberine mitigates high glucose-potentiated platelet aggregation and apoptosis by modulating aldose reductase and NADPH oxidase activity [J].Free Radic Biol Med, 2018, 130: 196-205.

MA H, HU Y, ZOU Z, et al. Antihyperglycemia and antihyperlipidemia effect of protoberberine alkaloids from Rhizoma Coptidis in HepG2 cell and diabetic KK-Ay mice [J].Drug Dev Res, 2016, 77(4): 163-170.

郑建雷，李忠杰，孙渴望，等．三七总皂甙对高脂饮食糖尿病大鼠炎症因子和主动脉脂质代谢的影响[J]．浙江医学，2018，40(15)：1662-1666．

侯宏，李学刚，李平，等．黄连生物碱的提取纯化及降脂作用[J]．长春中医药大学学报，2011，27(1)：7-8．

CHEN Z H, LI J, LIU J, et al. Saponins isolated from the root of Panax notoginseng showed significant anti-diabetic effects in KK-Ay mice [J].Am J Chin Med, 2008, 36(5): 939-951.

郭爽，张强，田志浩，等．三七总皂苷联用阿司匹林对后者抗血小板凝集作用的影响[J]．中国实验方剂学杂志，2018，24(9)：116-120．

Henry M L, Davidson L B, Wilson J E, et al. Whole blood aggregation and coagulation in db/db and ob/ob mouse models of type 2 diabetes [J].Blood Coagul Fibrinolysis, 2008, 19(2): 124-134.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Evaluation of Compatibility Proportion and Antidepressant Mechanism of Bupleuri Radix-Scutellariae Radix Based on Uniform Design

Effect of Dose Compatibility of Main Chemical Components from Astragali Radix- Angelicae Sinensis Radix on Proliferation of VSMCs Based on Uniform Design

New Progress in Mechanism of Salviae Miltiorrhizae Radix et Rhizoma in Treatment of Diabetes and Its Complications

Comparative Study on Hepatotoxicity of Psoraleae Fructus in Rats and Mice

Effect of Combination of Anthraquinone Glucosides from Cassia Seed and Shanzha Total Triterpenoid Acid on Activity of High Density Lipoprotein Receptor in Hyperlipidemic Rabbits

Related Author

Li-ping ZHOU

Guo-chun LI

Chen-yi-yu ZHANG

Fan ZHAO

Ya-qin LI

Shi-ping MA

Rong QU

CHEN Lingbo

Related Institution

School of Medicine and Life Sciences, School of Basic Medicine, Nanjing University of Chinese Medicine

School of Chinese Materia Medica, China Pharmaceutical University

School of Mathematics，Southwest Jiaotong University

College of Integrated Traditional Chinese and Western Medicine，Medical School， Hunan University of Chinese Medicine

Heilongjiang University of Chinese Medicine

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰