Effect of Menthol on Proliferation, Migration and Expressions of IL-8, CXCL-12 and VEGF in Hepatoma HepG2 Cells

Xing-kui TAO; Xing-tao ZHANG; Hai-chao WANG; Hong DUAN; Jin CHENG

doi:10.13422/j.cnki.syfjx.20192124

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Effect of Menthol on Proliferation, Migration and Expressions of IL-8, CXCL-12 and VEGF in Hepatoma HepG2 Cells

Pharmacology | 更新时间：2021-02-09

- Effect of Menthol on Proliferation, Migration and Expressions of IL-8, CXCL-12 and VEGF in Hepatoma HepG2 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 21, Pages: 60-65(2019)
- 作者机构：
  
  宿州学院　生物与食品工程学院，宿州市天然产物与功能性食品工程技术研究中心，安徽　宿州　 234000
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192124
  CLC： R22; R242; R2-031;R285.5
- Published：05 November 2019，
  
  Published Online：17 July 2019，
  
  Received：31 May 2019，
- 稿件说明：
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Xing-kui TAO, Xing-tao ZHANG, Hai-chao WANG, et al. Effect of Menthol on Proliferation, Migration and Expressions of IL-8, CXCL-12 and VEGF in Hepatoma HepG2 Cells. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(21):60-65(2019)
DOI：

Xing-kui TAO, Xing-tao ZHANG, Hai-chao WANG, et al. Effect of Menthol on Proliferation, Migration and Expressions of IL-8, CXCL-12 and VEGF in Hepatoma HepG2 Cells. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(21):60-65(2019) DOI： 10.13422/j.cnki.syfjx.20192124.

摘要

目的：

体外实验观察不同剂量薄荷醇对肝癌HepG2细胞增殖、迁移及白细胞介素-8(IL-8)，趋化因子-12(CXCL-12)和血管内皮生长因子(VEGF)表达的影响，阐释薄荷醇抗肝癌的作用及其相关机制。

方法：

设立薄荷醇(25，50，100，200 μmol·L

－1

)组以及空白组作用于肝癌HepG2细胞，利用细胞增殖与毒性检测(CCK-8)法和5-乙炔基-2′脱氧尿嘧啶核苷(EDU)检测方法检测薄荷醇对HepG2细胞增殖的影响，利用迁移实验(Transwell)检测薄荷醇对HepG2细胞迁移能力的影响，利用实时荧光定量聚合酶链式反应检测薄荷醇处理HepG2细胞炎症和趋化因子IL-8，CXCL-12 mRNA表达水平，通过蛋白免疫印迹法(Western blot)检测薄荷醇处理对HepG2细胞中VEGF蛋白的表达水平。

结果：

与空白组比较，薄荷醇(25，50，100，200 μmol·L

－1

)在体外对HepG2细胞增殖和迁移均有抑制作用。薄荷醇100 μmol·L

－1

时抑制作用明显(

0.05)；薄荷醇(50，100，200 μmol·L

－1

)对HepG2细胞迁移能力有明显抑制作用(

0.05)，呈剂量依赖性。与空白组比较，薄荷醇(100，200 μmol·L

－1

)均能显著抑制HepG2细胞中的IL-8，CXCL-12 mRNA和VEGF蛋白的表达水平(

0.05)，进一步证实薄荷醇通过抑制炎症趋化因子起到抗癌作用。

结论：

薄荷醇在体外对肝癌HepG2细胞增殖、迁移具有明显抑制作用，潜在机制可能与其抑制细胞IL-8，CXCL-12，VEGF的表达有关。此结论为薄荷醇抗肝癌作用的阐释提供了实验依据。

Abstract

Objective:

To study the effect of menthol on proliferation

migration and expressions of interleukin-8(IL-8)

C-X-C motif chemokine-12(CXCL-12) and vascular endothelial growth factor(VEGF) in hepatoma HepG2 cells

in vitro

in order to elucidate the anti-liver cancer activity of menthol and relevant mechanisms.

Method:

Different concentrations of menthol (25

100

200 μmol·L

-1

) and blank group were applied to Hepatoma HepG2 cells. Cell counting kit-8(CCK-8) and EDU were used to detect the proliferation effect of menthol on HepG2 cell. Transwell experiment was used to detect the migration effect of menthol on HepG2 cell. Real-time fluorescent quantitative polymerase chain reaction technique was used to detect the inflammatory chemokines IL-8 and CXCL-12 mRNA expression levels in HepG2 cell. Western blot was used to detect the expression level of VEGF in HepG2 cells treated with menthol.

Result:

Compare with the blank group

menthol (25

100

200 μmol·L

-1

) significantly inhibited proliferation and migration of HepG2 cells

in vitro

. When the concentration of menthol was 100 μmol·L

-1

microns

the inhibitory effect was significant (

0.05). Menthol significantly inhibited the migration of HepG2 cells. The higher the concentration of menthol was

the less the number of cell migration was. Compared with the blank group

menthol (100

200 μmol·L

-1

) significantly inhibited the expression levels of IL-8

CXCL-12 mRNA and VEGF protein in HepG2 cells (

0.05)

indicating that menthol is an anticancer agent by inhibiting inflammation and chemokines.

Conclusion:

Menthol has an inhibitory effect on the proliferation and migration of hepatoma HepG2 cells

in vitro

and the potential anti-liver cancer mechanism might be related to the inhibition of IL-8

CXCL-12 and VEGF expressions in the cells. This conclusion provides the experimental basis for elucidating the effect of menthol against liver cancer.

关键词

薄荷醇细胞增殖细胞迁移HepG2细胞

Keywords

mentholcell proliferationcell migrationHepG2 cells

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