Cloning and Sequence Analysis of Pathogenesis Related Protein 1-like Gene from Sorbus aucuparia

Ya-hui LIU; Jia-xing LI; Sheng WANG; Tie-lin WANG; Jie YUAN; Lan-ping GUO

doi:10.13422/j.cnki.syfjx.20192348

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Cloning and Sequence Analysis of Pathogenesis Related Protein 1-like Gene from Sorbus aucuparia

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- Cloning and Sequence Analysis of Pathogenesis Related Protein 1-like Gene from Sorbus aucuparia
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 25, Issue 21, Pages: 118-123(2019)
- 作者机构：
  
  1.中国中医科学院　中药资源中心　道地药材国家重点实验室培育基地，北京　 100700；
  2.中国中医科学院　博士后科研流动站，北京　 100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.20192348
  CLC： R22; R931; R282; Q523; Q2; Q342
- Published：05 November 2019，
  
  Published Online：08 August 2019，
  
  Received：17 July 2019，
- 稿件说明：
扫描看全文
Ya-hui LIU, Jia-xing LI, Sheng WANG, et al. Cloning and Sequence Analysis of Pathogenesis Related Protein 1-like Gene from Sorbus aucuparia. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(21):118-123(2019)
DOI：

Ya-hui LIU, Jia-xing LI, Sheng WANG, et al. Cloning and Sequence Analysis of Pathogenesis Related Protein 1-like Gene from Sorbus aucuparia. [J]. Chinese Journal of Experimental Traditional Medical Formulae 25(21):118-123(2019) DOI： 10.13422/j.cnki.syfjx.20192348.

摘要

目的：

探究欧洲花楸类病程相关蛋白1(SaPR1-like)在响应生物胁迫中的功能。

方法：

克隆得到了

SaPR

基因全长序列，并对该序列进行生物信息学分析；通过实时荧光定量聚合酶链式反应(PCR)方法检测

SaPR

在欧洲花楸悬浮细胞响应harpin蛋白胁迫后的表达特征。

结果：

克隆得到的

SaPR

基因包含1个完整开放阅读框，编码161个氨基酸。该编码蛋白质亲水性强且稳定，具有跨膜结构和信号肽，属于分泌蛋白。氨基酸序列比对分析发现，SaPR1-like蛋白C端具有高度保守结构域[富含半胱氨酸的分泌蛋白、抗原5和致病性相关蛋白1肽段(CAPE)]，推测其在欧洲花楸响应生物胁迫中发挥作用。harpin蛋白诱导欧洲花楸悬浮细胞24 h后，

SaPR

基因表达量显著提高。

结论：

克隆得到了欧洲花楸

SaPR

基因，并证明其在响应生物胁迫时表达和积累，可为进一步阐释欧洲花楸响应生物胁迫机制奠定基础和提供参考。

Abstract

Objective:

To explore the functions of pathogenesis related protein 1-like (PR1-like) in suspension cell of

Sorbus aucuparia

(SaPR1-like) under biotic stress.

Method:

The full sequence of

SaPR

gene was cloned and analyzed by multiple bioinformatic tools. The expression of

SaPR

in the suspension cell of

aucuparia

in response to harpin protein stress was analyzed by real-time fluorescence quantitative polymerase chain reaction (PCR).

Result:

SaPR

gene was identified and cloned

which contained a complete open reading frame and encoded 161 amino acids. The coding protein was hydrophilic and stable

had transmembrane structure and signal peptide

and belonged to secretory protein. Amino acid sequence alignment data indicated that the C-terminal of SaPR1-like contained a highly conserved domain [cysteine-rich secretory protein

antigen 5

and pathogenesis-related 1 peptides (CAPE)]

which could induce defense genes to produce immune responses against biotic stresses.

SaPR

gene expression was significantly increased after harpin protein induced suspension cell of

aucuparia

for 24 h.

Conclusion:

SaPR

gene derived from the

1 family is found to induce significant responses to biotic elicitors in

aucuparia

. This study highlights a role for PR1 in immune signaling and suggests the potential application of PR1 in efforts to defeat biotic stress in plants.

关键词

病程相关蛋白1欧洲花楸悬浮细胞基因克隆生物胁迫序列分析荧光定量分析

Keywords

pathogenesis-related protein 1Sorbus aucuparia; suspension cellgene cloningbiotic stresssequence analysisfluorescence quantitative analysis

references

刘亚辉，李佳兴，周良云，等．花楸属植物的研究进展[J]．中国实验方剂学杂志，2019，25(11)：195-205．

黄蕾，肖文娟，王升，等．欧洲花楸及其悬浮细胞在植物抗逆性研究中的应用[J]．中国现代中药，2016，18(10)：1359-1364．

黄蕾，肖文娟，杨光，等．酵母提取物诱导欧洲花楸悬浮细胞合成次生代谢产物的机制探究[J]．中国中药杂志，2014，39(11)：2019-2023．

李佳兴，莫歌，周良云，等．欧洲花楸糖基转移酶基因的全长克隆与表达分析[J]．中国实验方剂学杂志，2019，25(5)：167-172．

ZHOU L Y, YANG J, YANG G, et al. Biphenyl phytoalexin in Sorbus pohuashanensis suspension cell induced by yeast extract [J].Molecules, 2016, 21(9): E1180.

Khalil M N, Brandt W, Beuerle T, et al. O-Methyltransferases involved in biphenyl and dibenzofuran biosynthesis [J].Plant J, 2015, 83(2): 263-276.

郭兰萍，吕朝耕，王红阳，等．中药生态农业与几种相关现代农业及GAP的关系[J]．中国现代中药，2018，20(10)：1179-1188．

van Loon L C, van Kammen A. Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var.”Samsun” and ”Samsun NN”. II.Changes in protein constitution after infection with tobacco mosaic virus [J].Virology, 1970, 40(2): 190-211.

Alexander D, Goodman R M, Gut-Rella M, et al. Increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a [J].Proc Natl Acad Sci USA, 1993, 90(15): 7327-7331.

Breen S, Williams S J, Outram M, et al. Emerging insights into the functions of pathogenesis-related protein 1 [J].Trends Plant Sci, 2017, 22(10): 871-879.

CHEN Y L, Lee C Y, Cheng K T, et al. Quantitative peptidomics study reveals that a wound-induced peptide from PR-1 regulates immune signaling in tomato [J].Plant Cell, 2014, 26(10): 4135-4148.

van Loon L C, Rep M, Pieterse C M. Significance of inducible defense-related proteins in infected plants [J].Annu Rev Phytopathol, 2006, 44: 135-162.

Boccardo N A, Segretin M E, Hernandez I, et al. Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials [J].Sci Rep, 2019, 9(1): 2791.

Wiesner-Hanks T, Nelson R. Multiple disease resistance in plants [J].Annu Rev Phytopathol, 2016, 54: 229-252.

Moscou M J, van Esse H P. The quest for durable resistance [J].Science, 2017, 358(6370): 1541-1542.

Rodriguez-Moreno L, SONG Y, Thomma B P. Transfer and engineering of immune receptors to improve recognition capacities in crops [J].Curr Opin Plant Biol, 2017, 38: 42-49.

Christou P, Twyman R M. The potential of genetically enhanced plants to address food insecurity [J].Nutr Res Rev, 2004, 17(1): 23-42.

Moosa A, Farzand A, Sahi S T, et al. Transgenic expression of antifungal pathogenesis-related proteins against phytopathogenic fungi-15 years of success [J].Isr J Plant Sci, 2017, doi: 10.1080/07929978.2017.1288407http://doi.org/10.1080/07929978.2017.1288407.

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