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HPLC测定决明子中3种萘骈吡喃酮苷含量
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国家自然科学基金面上项目(81173552)


Determination of Three Naphthopyrone Glycosides in Cassia obtusifolia by HPLC
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    摘要:

    目的:建立HPLC同时测定决明子中红镰霉素龙胆二糖苷、决明子苷、决明子苷C含量的方法。方法:采用HPLC,Dionex Acclaim C18柱(4.6 mm×250 mm,5 μm),流动相乙腈-四氢呋喃-1%冰醋酸(17:2:81),流速1 mL·min-1,柱温30 ℃,检测波长278 nm。结果:红镰霉素龙胆二糖苷、决明子苷、决明子苷C的进样量分别在0.218~1.090,0.188~0.940,0.200~1.000 μg和峰面积呈良好的线性关系,回归方程分别为Y=54.017X-0.344 6 (r=0.999 9),Y=78.63X-0.354(r=0.999 9),Y=74.098X-0.433 5 (r=0.999 9);平均加样回收率分别为98.43%(RSD 1.75%),101.89%(RSD 1.10%),102.46%(RSD 1.15%)。结论:该方法快速、灵敏、准确、可靠、重复性好,可用于中药决明子药材的质量控制。

    Abstract:

    Objective: To establish a high performance liquid chromatographic method (HPLC) for simultaneous determination of rubrofusarin gentiobioside, casside, cassiaside C in Cassia obtusifolia. Method: The HPLC with Dionex Acclaim C18(4.6 mm×250 mm, 5 μm) column was used, acetonitrile-THF-1% acetic acid (17 :2 :81) was used as a mobile phase, with flow rate of 1 mL·min-1, column temperature at 30 ℃ and detection wavelength at 278 nm. Result: Rubrofusarin gentiobioside, casside, cassiaside C showed good linearity (r=0.999 9) in the range of 0.218-1.09, 0.188-0.94, 0.200-1.000 μg, regressive equation were Y=54.017X-0.344 6 (r=0.999 9),Y=78.63X-0.354(r=0.999 9),Y=74.098X-0.433 5 (r=0.999 9)respectively;the average recoveries of the method were 98.43%, 101.89%, 102.46%, RSD were 1.75%, 1.10%, 1.15%. Conclusion: The method was proved to be simple, rapid, sensitive, precise, reliable and repeatable. It can be applied to the quality control of Semen Cassia.

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徐义龙,唐力英,周喜丹,周国洪,王祝举. HPLC测定决明子中3种萘骈吡喃酮苷含量[J].中国实验方剂学杂志,2014,20(5):54~56

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  • 收稿日期:2013-09-17
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  • 在线发布日期: 2014-02-24
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