欢迎访问《中国实验方剂学杂志》编辑部网站!
不同产地薏苡仁药效成分含量与体外抗肺癌活性的相关性分析
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金项目(81373979);江苏省中医药领军人才项目(LJ200913);江苏省自然科学基金面上项目(BK20141508)


Correlation Analysis Between Content and Anti-lung Cancer Activity of Active Ingredients in Coicis Semen from Different Areas
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的: 考察不同产地薏苡仁的药效成分含量及其对肺癌细胞的增殖抑制作用,阐明药效组分含量与药效的相关性,确定薏苡仁的最优产地. 方法: 采用HPLC-ELSD和紫外分光光度法测定5个产地薏苡仁中甘油三油酸酯和总甘油三酯的含量,通过四甲基偶氮唑盐比色法测定不同产地薏苡仁提取液对人肺癌细胞A549和SPC-A-1的抑制率. 结果: 河北、辽宁、福建、贵州、山东产地的薏苡仁中甘油三油酸酯质量分数分别为0.91%,1.14%,0.82%,0.77%,1.09%,总甘油三酯质量分数分别为14.83,10.88,7.85,5.26,7.71 mg·g-1.对A549的半数抑制浓度(IC50)分别为2.33,2.77,3.15,3.24,3.08 g·L-1,对SPC-A-1的IC50依次为1.64,2.15,2.63,3.01,2.99 g·L-1. 结论: 5个产地薏苡仁中指标成分均符合2010年版《中国药典》的规定.河北产样品中总甘油三酯含量最高,抗肺癌活性最强,提示抗肺癌药效与总甘油三酯含量呈正相关.

    Abstract:

    Objective: To investigate correlation between content and anti-proliferative effect against lung carcinoma cell line SPC-A-1 and A549 of active ingredients in Coicis Semen from five places. Method: HPLC-ELSD and UV spectrophotometry were employed to determine contents of glycerol trioleate and total triglycerides in Coicis Semen,respectively.In addition,MTT assay was performed to explore anti-proliferative effect of Coicis Semen extract from various origins towards SPC-A-1 and A549 cells. Result: The content of glycerol trioleate in Coicis Semen from Hebei,Liaoning,Fujian,Guizhou,Shandong province was 0.91%,1.14%,0.82%,0.77%,1.09%,the content of total triglycerides was 14.83,10.88,7.85,5.26,7.71 mg·g-1,respectively.IC50(median inhibitory concentration) against the lung carcinoma cell line A549 were 2.33,2.77,3.15,3.24,3.08 g·L-1,IC50 against SPC-A-1 were 1.64,2.15,2.63,3.01,2.99 g·L-1,respectively. Conclusion: All samples originated from five provinces are in line with standards of the 2010 edition of Chinese Pharmacopoeia.However,samples from Hebei province have the highest content of total triglycerides and the strongest anti-lung cancer activity,suggesting a positive correlation between active ingredients content and pharmacological activity.

    参考文献
    相似文献
    引证文献
引用本文

刘聪燕,黄萌萌,周静,陈彦,瞿鼎,马益华.不同产地薏苡仁药效成分含量与体外抗肺癌活性的相关性分析[J].中国实验方剂学杂志,2015,21(11):7~10

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2014-08-15
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2015-05-28
  • 出版日期:

地址:北京东直门内南小街16号

邮编:100700

电话:010-84076882

E-mail:syfjx_2010@188.com

中国实验方剂学杂志 ® 2021 版权所有

技术支持:北京勤云科技发展有限公司