Chinese Journal of Experimental Traditional Medical Formulae
Effect of Chinese Medicine Weichangshu on Mitochondrial in Diabetic Mellitus Rats Stomach Smooth Mucle Cells

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    【Abstract】Objective To study the mechanism of the chinese medicine Weichangshu on mitochondrial kinetic energy in diabetic mellitus rats stomach by measuring the changes of morphology, structure, function and protein of mitochondria. Methods: Standard Sprague Dawley rats with a mean weight of 150-170g were randomly divided into 4 groups, including a control group, DM model group, DM WeichangshuⅠgroup, DM Weichangshu Ⅱ group. The mitochondrial morphology and structure were detected using transmission electron microscopy. The changes of mitochondrial transmembrane potential, respiratory chain and mitochondrial protein were detected using flow cytometry, fluorescence labeling and Western Blot respectively. Results: Weichangshu could ameliorate the mitochondrial morphology, structure and respiratory chain in diabetic mellitus rats stomach. Mitochondrial transmembrane potential, ATP level and activity of cytochrome C oxidase were increased. The release of ROS was decreased. The expression of Bcl-2 up-regulated and bax down-regulated. Conclusion: Weichangshu could promoted the gastrointestinal motility, which might be related with the regulation of mitochondrial kinetic energy.

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  • Received:January 09,2010
  • Revised:January 09,2010
  • Adopted:February 11,2010
  • Online: June 28,2010
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