أساسيات محلول المياه الحية في مكافحة نقص الدماغ

  • role: First author第一作者
  • Affiliation:

    Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China

  • Email:ljt02052921@163.com
  • Introduction:刘憬曈,在读硕士,从事中药复杂作用解析研究,E-mail:ljt02052921@163.com
LIU Jingtong1,  
  • Affiliation:

    Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China

HU Shaowei1,  
  • Affiliation:

    Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China

CHANG Mengli1,  
  • Affiliation:

    Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China

XU Jing1,  
  • Affiliation:

    Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China

CAI Qingqing1,  
  • Affiliation:

    School of Pharmacy,Zunyi Medical University,Zunyi 563099,China

LI Xinghong2,  
  • Affiliation:

    Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China

TANG Liying1,  
  • role: Corresponding author通信作者
  • Affiliation:

    Hospital of Integrated Chinese and Western Medicine,Tianjin 300100,China

  • Email:hhwang1103@163.com
  • Introduction:王欢欢,博士,助理研究员,从事中药复杂作用解析研究,E-mail:hhwang1103@163.com; *
WANG Huanhuan3*,  
  • role: Corresponding author通信作者
  • Affiliation:

    Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China

  • Email:whw9905012@163.com
  • Introduction:吴宏伟,博士,研究员,从事中药质量控制与复杂作用解析研究,E-mail:whw9905012@163.com
WU Hongwei1*

ملخص

تقييم الغرض من تأثير صيدلاني للماء الحيوي على مقاومة نقص الدماغ ودراسة آلية حماية الأعصاب منه. الأساليب في هذه الدراسة ، تم تقسيم ذكور الفئران الحية SPF إلى مجموعة جراحية وهمية ، ومجموعة نموذجية ، ومجموعة مخفضة ومتوسطة وعالية الجرعة لمحلول الماء الحيوي (3 و 6 و 12 مل / كغ) ، ومجموعة دواء إيجابية لجسر ذهبي (4 مل / كغ) بناءً على الجدول العشوائي للأرقام. تم تحضير نموذج الجلطة الدماغية والإعادة التدفقية (MCAO/R) باستخدام طريقة التثبيط الثقيل ، ثم تم إعطاء العلاج بعد إعادة التدفق ، وكانت الجرعة مستمرة لثلاثة أيام. تم تقييم السلوك العصبي ، مساحة الجلطة الدماغية ، التلوين بالهيماتوكسيلين واليوه إيوسين (HE) ، التلوين بالنيسي ، التلوين بتقديس التمثيل الهرمي في الموقع الطرفي (TUNEL) ، وتقييم الفارق البروتين المناعي (Western blot) للتأثير المحمي لمحلول الماء الحيوي على إصابات MCAO/R الدماغية في الفئران. تم التعرف على مزايا تحسين إصابات الدماغ في الفئران بواسطة تقنيات البروتيوميكا ، مع التركيز على البروتين الكلوريدات المحفزة 1 (CLIC1) كبروتين رئيسي في شبكة التأثير. تم التحقق أيضًا من آلية ذلك بناءً على نموذج خلية كرومافينية لورم الفوقية المشيمية (PC12) المجهزة.-1

مفهوم

محلول المياه الحية، إصابات الدماغ ناتجة عن إعادة التدفق، الضغط النقصي للأوكسجين، الرد الالتهابي، البروتين الكلوريدات المحفزة 1 (CLIC1)

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