تقييم وتقييم نموذج فأر القلب الايشميكي IHD-فقر الهواء والرطوبة بناءً على تقنية البروتينوم

  • role: First author第一作者
  • Affiliation:

    Beijing University of Chinese Medicine,Beijing 100029,China

    Beijing Key Laboratory of Chinese Materia Pharmacology,Institute of Basic Medical Sciences, Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China

  • Email:qyzhang818@163.com
  • Introduction:E-mailqyzhang818@163.com
ZHANG Qiuyan12,  
  • Affiliation:

    Beijing Key Laboratory of Chinese Materia Pharmacology,Institute of Basic Medical Sciences, Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China

LI Ying2,  
  • Affiliation:

    Natural History Museum of China,Beijing 100050,China

GAO Yunxiao3,  
  • Affiliation:

    Beijing University of Chinese Medicine,Beijing 100029,China

    Beijing Key Laboratory of Chinese Materia Pharmacology,Institute of Basic Medical Sciences, Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China

HU Longxiao12,  
  • Affiliation:

    Beijing Key Laboratory of Chinese Materia Pharmacology,Institute of Basic Medical Sciences, Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China

YUAN Yue2,  
  • Affiliation:

    Beijing Key Laboratory of Chinese Materia Pharmacology,Institute of Basic Medical Sciences, Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China

CHEN Xiaoxiao2,  
  • Affiliation:

    Beijing Key Laboratory of Chinese Materia Pharmacology,Institute of Basic Medical Sciences, Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China

SHI Yali2,  
  • role: Corresponding author通信作者
  • Affiliation:

    Beijing University of Chinese Medicine,Beijing 100029,China

    Beijing Key Laboratory of Chinese Materia Pharmacology,Institute of Basic Medical Sciences, Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China

  • Email:reek2003@163.com
  • Introduction:E-mailreek2003@163.com
REN Junguo12*

ملخص

الهدف هو استكشاف حقن أيزوبروتيرين المحيطية لتأسيس طريقة بناء نموذج الفأر الصغير ذو الإنتاج المنخفض من مرض القلب الايشميكي (IHD) مع الفقر الهواء والرطوبة وبناءها وأساسها البيولوجي. الأسلوب: تم تقسيم 144 فأر ذكر C57BL/6J على أساس الوزن إلى 3 مجموعات عادية و 9 مجموعات نموذجية، كل مجموعة تحتوي على 12 فأراً. باستخدام جرعة حقن أيزوبروتيرين المحيطية (5 و 10 و 15 مغ / كغ / دائئ) ، وفترة الحقن (4 و 7 و 14 يومًا) كعوامل ، تم بناء جدول كر ارثي ثلاثي التحقيقات ، و 3 مجموعات عادية تلقي ملح فيزيائي معادل في الوقت المحدد. بعد انتهاء النمذجة ، تم فحص وزن الجسم لفئة الفئة وكمية الماء لمدة 24 ساعة وقوة الالتقاط والنشاط الذاتي ، وتم اختبار وظيفة القلب بالصوت الخفقاني الفوقي ، وتم اختبار مستويات النيفرين (NE) والـ CAMP والجمبريل الحلقي الرباعي (cGMP) في المصل بطريقة التحليل الامتصاصي المرتبط بالانزيمات (ELISA) ، وتم اختبار محتوى نواة العضلة القلبية بطريقة الكيمياء الحيوية ؛ ، ومراقبة التلطيخ بواسطة سومي واليوز (HE) المظهر التشريحي لتغيير الأنسجة العضلية ؛ التحليل المرئي والتحليل الاحصائي المشتق من التجربة المعينة وتحليل التباين تم اختيار أفضل ظروف نموذج الفأر الصغير مع IHD-فقر الهواء والرطوبة ؛ استخدام جمع البيانات بما يعتمد على الطيف الكتلي الموجه (DDA) وتكنولوجيا البروتينوم خلال الكمية الفردية ، واختبار التحليل الحيوي للمعلومات ، واستكشاف الآليات البايولوجية الكامنة لنموذج IHD مع فقر الهواء والرطوبة.

مفهوم

التجربة المعينة ، أيزوبروتيرين ، الفقر الهوائي والرطوبة ، مرض القلب الايشميكي ، نموذج الفأر الصغير ، تقنية البروتينوم

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