Mechanismus der Schädigung der Spermatogenese und Spermienqualität durch Propylthiouracil über den endoplasmatischen Retikulumstress und Eingriff von Shugan Bushen Yulin Tang bei Ratten

  • role: First author第一作者
  • Affiliation:

    School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China

    Hubei Shizhen Laboratory, Wuhan 430065, China

  • Email:1653600142@qq.com
  • Introduction:西E-mail1653600142@qq.com
MENG Yuhao12,  
  • Affiliation:

    Hubei Shizhen Laboratory, Wuhan 430065, China

    Laboratory Animal Center, Hubei University of Chinese Medicine, Wuhan 430065, China

JIANG Xiaocui23,  
  • role: Corresponding author通信作者
  • Affiliation:

    Hubei Shizhen Laboratory, Wuhan 430065, China

    Laboratory Animal Center, Hubei University of Chinese Medicine, Wuhan 430065, China

  • Email:531637551@qq.com
  • Introduction:西E-mail531637551@qq.com
XIAO Min23*,  
  • role: Corresponding author通信作者
  • Affiliation:

    School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China

    Hubei Shizhen Laboratory, Wuhan 430065, China

  • Email:43382275@qq.com
  • Introduction:E-mail43382275@qq.com *
WANG Chaoyang12*

Resümee

Ziel ist es, den Mechanismus zu untersuchen, durch den die Shugan Bushen Yulin Tang-Formel die durch Propylthiouracil (PTU) verursachte Störung der Spermatogenese und die Verschlechterung der Spermienqualität bei Ratten verbessert, basierend auf dem Signalweg des endoplasmatischen Retikulumstresses (ERS). Methode: 60 männliche Ratten wurden zufällig in Kontroll-, Modell-, Niedrig-, Mittel- und Hochdosis-Gruppen von Shugan Bushen Yulin Tang sowie eine Levocarnitin-Gruppe mit jeweils 10 Tieren eingeteilt. Mit Ausnahme der Kontrollgruppe erhielten die anderen Gruppen 12 Tage lang PTU per orale Verabreichung zur Modellierung. Anschließend folgte eine 28-tägige Behandlung. Organindizes, histopathologische Veränderungen, Hormonspiegel, oxidativer Stress, Apoptose und ERS-bezogene Proteinexpression wurden bewertet. Die Ergebnisse zeigten eine deutliche Verschlechterung in der Modellgruppe, die durch die Behandlung verbessert wurde. Fazit: Shugan Bushen Yulin Tang verbessert PTU-induzierte Störungen der Spermatogenese möglicherweise durch Reparatur oxidativer Schäden und Regulation des PERK/EIF2α/ATF4-Signalwegs des ERS.

Schlüsselwort

Shugan Bushen Yulin Tang; Propylthiouracil; endoplasmatischer Retikulumstress; PERK-Kinase/eIF2α-Initiationsfaktor/ATF4-Transkriptionsaktivator; Spermienqualität

References

  1. 1.
    LEVINE H,JØRGENSEN N,MARTINO-ANDRADE A,et al. Temporal trends in sperm count:A systematic review and meta-regression analysis of samples collected globally in the 20th and 21st centuries[J].Hum Reprod Update,2023,29 (2):157-176.
  2. 2.
    ANELLI V,GATTA E,PIROLA I,et al. Thyroid impairment and male fertility:A narrative review of literature[J].Aging Male,2024,27 (1):2310303.
  3. 3.
    ZHANG X,WANG X,HU H,et al. Prevalence and trends of thyroid disease among adults, 1999-2018[J].Endocr Pract,2023,29(11):875-880.
  4. 4.
    ROMANO R M,GOMES S N,CARDOSO N C,et al. New insights for male infertility revealed by alterations in spermatic function and differential testicular expression of thyroid-related genes[J].Endocrine,2017,55 (2):607-617.
  5. 5.
    FRANçOIS XAVIER K N,PATRICK BRICE D D,MODESTE W N,et al.Preventive effects of Aframomum melegueta extracts on the reproductive complications of propylthiouracil-induced hypothyroidism in male rat[J].Andrologia,2019,51 (7):e13306.
  6. 6.
    WEI W, CAO J G. Clinical observation of Shugan Bushen Yulin decoction in treatment of male infertility caused by liver-depression and kidney-deficiency syndrome [J].Liaoning J Tradit Chin Me,2020,47(10):110-112.
  7. 7.
    REN C X, ZHU Z M, GU C Y, et al. Toxic effects and molecular mechanism of triptolide on testis and epididymal tissue in mice via inhibiting PGC-1α[J]. Chin J Bioprocess Eng, 2021,19(6):649-656.
  8. 8.
    BARATI E,NIKZAD H,KARIMIAN M.Oxidative stress and male infertility:Current knowledge of pathophysiology and role of antioxidant therapy in disease management[J].Cell Mol Life Sci,2020,77 (1):93-113.
  9. 9.
    TANG Y,ZHOU X,CAO T,et al. Endoplasmic reticulum stress and oxidative stress in inflammatory diseases[J].DNA Cell Biol,2022,41 (11):924-934.
  10. 10.
    XIE Y,E J,CAI H,et al.Reticulon-1A mediates diabetic kidney disease progression through endoplasmic reticulum-mitochondrial contacts in tubular epithelial cells[J].Kidney Int,2022,102 (2):293-306.
  11. 11.
    ASLAM H,ROSIEPEN G,KRISHNAMURTHY H,et al. The cycle duration of the seminiferous epithelium remains unaltered during GnRH antagonist-induced testicular involution in rats and monkeys[J].J Endocrinol,1999,161 (2):281-288.
  12. 12.
    WAKUI S,SATO D,OKAYAMA Y,et al. Prenatal exposure to di(n-butyl) phthalate delays the spermatogenic cycle in rats: Investigation using a BrdU-injection method[J].Reprod Toxicol,2022,109:135-146.
  13. 13.
    JIANG X C,TIAN D Z,LIU Q,et al.Shenling Baizhusan improves spermatogenesis in hyperuricemia oligoasthenos-permia mice by regulating Nrf2/ARE pathway[J].Chin J Exp Tradit Med Form,2023,29(15):22-30.
  14. 14.
    ILACQUA A,IZZO G,EMERENZIANI G P,et al. Lifestyle and fertility:The influence of stress and quality of life on male fertility[J].Reprod Biol Endocrinol,2018,16 (1):115.
  15. 15.
    CHEN Q H, LI B, ZHAO W X, et al. Bushen Yanjing soup combined with levocarnitine for the treatment of oligospermia and asthenospermia[J].Chin J Hum Sex,2018,27(5):105-107.
  16. 16.
    ZHANG J W,YAN B,GUO B D.Multiple disciplinary teamwork guidelines of integrated traditional Chinese medicine & western medicine for male infertility (2023 edition)[J].Chi J Androl, 2023, 37(2):13-19.
  17. 17.
    ZHAO M, LEI Z W, ZHANG J W, et al. Experience of ZHOU Anfang in treating male infertility with the method of Bushen and Shugan[J].Lishizhen Med Mater Med Res, 2023,34(3):722-724.
  18. 18.
    ZHENG J Z,HAN L Z,YIN Y H,et al.CUI Yun's experience in treating immune male infertility[J].Beijing J Tradit Chin Med, 2023, 42(12):1335-1338.
  19. 19.
    XIAO M, WANG W, WEI W, et al. Shugan Bushen Yulin decoction regulates VDAC2 gene methylation to affect sperm mitochondrial function and improve sperm quality in asthenospermia rats[J].Chin J Exp Tradit Med Form, 2021, 27(23):72-79.
  20. 20.
    CHEN S H, XIAO M, JIANG X Y, et al. Bushen Shugan Yulin decoctio regulating autophagy flow and improving sperm vitality through PINK1/Parkin pathway[J].Chin Arch Tradit Chin Med, 2024, 42(3):178-183.
  21. 21.
    JIANG X C, XIAO M, ZHAO M, et al. Shugan Bushen Yulin decoctio can improve sperm quality of liver depression and kidney deficiency type of oligasthenospermia mice by regulating Keap1/Nrf2/GPX4 pathway to inhibit ferroptosis of testicular cells[J].Chin Arch Tradit Chin Med, 2024, 42(5):46-51.
  22. 22.
    KOOHPEYMA F,GHOLIZADEH F,HAFEZI H,et al. The protective effect of L-carnitine on testosterone synthesis pathway,and spermatogenesis in monosodium glutamate-induced rats[J].BMC Complement Med Ther,2022,22 (1):269.
  23. 23.
    YARI A,ASADI M H,BAHADORAN H,et al. Cadmium toxicity in spermatogenesis and protective effects of L-carnitine in adult male rats[J].Biol Trace Elem Res,2010,137 (2):216-225.
  24. 24.
    WANG J,BAO B,MENG F,et al. The mechanism analysis using PI3K/Akt pathway for the effects of levocarnitine in the treatment of spermatogenic dysfunction[J].Andrologia,2022,54(1):e14290.
  25. 25.
    MINTZIORI G,KITA M,DUNTAS L,et al. Consequences of hyperthyroidism in male and female fertility:Pathophysiology and current management[J].J Endocrinol Invest,2016,39 (8):849-853.
  26. 26.
    RAJENDER S,MONICA M G,WALTER L,et al. Thyroid, spermatogenesis, and male infertility[J].Front Biosci (Elite Ed),2011,3 (3):843-855.
  27. 27.
    PANAHANDEH F,FEIZI F,POURGHASEM M,et al. Hypothyroidism and Fertility:An animal model follows up in the second-generation[J].Cell J,2022,24 (3):148-154.
  28. 28.
    SARKAR D,SINGH S K.Neonatal hypothyroidism affects testicular glucose homeostasis through increased oxidative stress in prepubertal mice:Effects on GLUT3, GLUT8 and Cx43[J].Andrology,2017,5 (4):749-762.
  29. 29.
    ZHAO S,TANG L,FU J,et al.Subclinical hypothyroidism and sperm DNA fragmentation:A cross-sectional study of 5401 men seeking infertility care[J].J Clin Endocrinol Metab,2022,107 (10):e4027-e4036.
  30. 30.
    SAITO M,OKAMATSU-OGURA Y. Thermogenic brown fat in humans:Implications in energy homeostasis, obesity and metabolic disorders[J].World J Mens Health,2023,41 (3):489-507.
  31. 31.
    WEI Y,HAN C,LI S,et al. Cuscuta chinensis flavonoids down-regulate the DNA methylation of the H19/Igf2 imprinted control region and estrogen receptor alpha promoter of the testis in bisphenol A exposed mouse offspring[J].Food Funct,2020,11 (1):787-798.
  32. 32.
    TAO J L, WANG X, WEI Y Y, et al. The protective effect of Cuscuta chinensis flavonoids on abnormal secretion of androgen in mouse Leydig cells induced by bisphenol A[J]. Prog Vet Med, 2019, 40(5):71-75.
  33. 33.
    SHI G J,ZHENG J,WU J,et al. Protective effects of Lycium barbarum polysaccharide on male sexual dysfunction and fertility impairments by activating hypothalamic pituitary gonadal axis in streptozotocin-induced type-1 diabetic male mice[J].Endocr J,2017,64 (9):907-922.
  34. 34.
    ZHANG Y,SHEN N,QI L,et al.Efficacy of Schizandra chinensis polysaccharide on cyclophosphamide induced dyszoospermia of rats and its effects on reproductive hormones[J].Chin J Integr Tradit West Med, 2013, 33(3):361-364.
  35. 35.
    LIU W,HOU C H,LI J,et al.Study on improvement effects and mechanism of Astragalus complanatus total flavonoids on reproductive dysfunction model male rats[J].China Pharm, 2020,31(15):1854-1860.
  36. 36.
    WANG S C,LI B K,KAN Y X,et al.Duzhong Butiansu Capsules improve adenine-induced reproductive dysfunction in male rats[J].Natl J Androl, 2020, 26(1):63-73.
  37. 37.
    KRASSAS G E,PERROS P.Thyroid disease and male reproductive function[J].J Endocrinol Invest,2003,26(4):372-380.
  38. 38.
    CORPUZ-HILSABECK M,MOHAJER N,CULTY M. Dysregulation of immature sertoli cell functions by exposure to acetaminophen and genistein in rodent cell models[J].Cells,2023,12 (13):1804.
  39. 39.
    YANG M,LIU C,JIANG N,et al. Endoplasmic reticulum homeostasis:A potential target for diabetic nephropathy[J].Front Endocrinol (Lausanne),2023,14:1182848.
  40. 40.
    CHEN S M,ZHAO H X,YANG Y,et al.Changes of endoplasmic reticulum stress signaling pathway during process of apoptosis in testicular spermatogenic cells of mice induced by perfluorooctanoic acid[J].Asian J Ecotoxicol,2023, 18(5):207-217.
  41. 41.
    ERBAŞ E,GELEN V,KARA H,et al.Silver nanoparticles loaded with oleuropein reduce doxorubicin-induced testicular damage by regulating endoplasmic reticulum stress,and apoptosis[J].Biol Trace Elem Res,2024,202 (10):4687-4698.
  42. 42.
    HETZ C,ZHANG K,KAUFMAN R J.Mechanisms, regulation and functions of the unfolded protein response[J].Nat Rev Mol Cell Biol,2020,21 (8):421-438.
  43. 43.
    KIM J H,PARK S J,KIM T S,et al. Testicular hyperthermia induces unfolded protein response signaling activation in spermatocyte[J].Biochem Biophys Res Commun,2013,434 (4):861-866.
  44. 44.
    YIN L,DAI Y,CUI Z,et al.The regulation of cellular apoptosis by the ROS-triggered PERK/EIF2α/chop pathway plays a vital role in bisphenol A-induced male reproductive toxicity[J].Toxicol Appl Pharmacol, 2017,314:98-108.

Lesen Sie die ganze Passage

The above content is generated by Large Model Translation. The translated content is for reference only. We do not assume any commercial or legal responsibilty for any consequences arising from the use of our website