Früher|Nächster

Therapeutic Effect of Jidesheng Sheyao Tablets on VZV-induced Skin Vesicles in Guinea Pigs and Postherpetic Neuralgia in Rats

  • role： First author第一作者
  • Affiliation：

    Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100029，China

  • Email：3211908407@qq.com
  • Introduction：谢锐，在读硕士，从事中药药理研究，E-mail：3211908407@qq.com
XIE Rui,  
  • Affiliation：

    Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100029，China

GUO Shanshan,  
  • Affiliation：

    Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100029，China

GENG Zihan,  
  • Affiliation：

    Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100029，China

BAO Lei,  
  • Affiliation：

    Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100029，China

ZHAO Ronghua,  
  • Affiliation：

    Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100029，China

LI Shuran,  
  • Affiliation：

    Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100029，China

SUN Qiyue,  
  • Affiliation：

    Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100029，China

WANG Xinwei,  
  • Affiliation：

    Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100029，China

ZHANG Jingsheng,  
  • Affiliation：

    Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100029，China

CUI Xihe,  
  • role： Corresponding author通信作者
  • Affiliation：

    Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100029，China

  • Email：xlcui@icmm.ac.cn
  • Introduction：崔晓兰，博士，研究员，从事中药抗病毒及抗感染药理研究，E-mail：xlcui@icmm.ac.cn
CUI Xiaolan*,  
  • role： Corresponding author通信作者
  • Affiliation：

    Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100029，China

  • Email：jsun@icmm.ac.cn
  • Introduction：孙静，博士，副研究员，从事中药抗病毒及抗感染药理研究，E-mail：jsun@icmm.ac.cn；
SUN Jing*

Resümee

ObjectiveThis paper aims to investigate the therapeutic effects of Jidesheng Sheyao tablets on varicella-zoster virus （VZV） and its associated postherpetic neuralgia （PHN） to provide experimental evidence for the clinical application and secondary development of Jidesheng Sheyao tablets.MethodsFifty-six guinea pigs were randomly divided into seven groups according to their body weight， namely the normal group， the model group， the positive control group， the high-dose group， medium-dose group， and low-dose group of Jidesheng Sheyao tablets （1.92， 0.96， 0.48 g·d-1）， and the group treated with oral administration combined with topical application of Jidesheng Sheyao tablets （0.96 g·d-1 + 1.2 g·kg-1·d-1）. The skin on the back of the guinea pigs in each group was depilated and then abraded with sandpaper. Except for the normal group， 200 μL of VZV solution was dropped on the damaged parts of the back of the guinea pigs in the other groups， and the infection lasted for 2 consecutive days. The drug administration started 2 hours after the infection on the first day and lasted for 7 days. The pathological changes of the back of the guinea pigs in each group were observed every day starting from the second day after the infection. On the 7th day， the guinea pigs were sacrificed by CO2 anesthesia. The locally infected skin was taken， and the viral DNA nucleic acid load was detected by real-time polymerase chain reaction （Real-time PCR）. The pathological histology examination was carried out after hematoxylin-eosin （HE） staining. Seventy rats were randomly divided into seven groups according to their body weight， namely the normal group， the model group， the positive control group， the high-dose group， medium-dose group， and low-dose group of Jidesheng Sheyao tablets （1.08， 0.54， 0.27 g·d-1）， and the group treated with oral administration combined with topical application of Jidesheng Sheyao tablets （0.54 g·d-1 + 1.2 g·kg-1·d-1）. The rats in each group （except the normal group） were subcutaneously inoculated with 50 μL of VZV suspension between the web of the first and second fingers of the left forelimb. The skin on the back of the rats was depilated， and the drug administration started 2 hours after the infection and lasted for 10 days. The mechanical withdrawal threshold of the paws of the rats was detected by a Von Frey filament algometer before inoculation and on the 1st， 3rd， 6th， 8th， and 10th days after inoculation， and the thermal withdrawal reflex latency of the paws of the rats was detected by a hot and cold plate algometer. On the 10th day after the virus inoculation， the rats were anesthetized after the behavioral examination， and the dorsal root ganglia of the spinal cord and spinal cord segments were taken. The contents of substance P （SP）， neurokinin （NK）， tumor necrosis factor-α （TNF-α）， and interleukin-1β （IL-1β） in the dorsal root ganglia of the spinal cord and spinal cord were detected by enzyme-linked immunosorbent assay （ELISA）.ResultsCompared with those in the normal group， the guinea pigs in the model group had obvious skin herpes lesions （P<0.01）. The viral nucleic acid load was high （P<0.01）， and there were disorganized subcutaneous cellular structures and obvious infiltration of inflammatory cells and cell necrosis （P<0.01）. The mechanical withdrawal threshold of the paws and the thermal withdrawal reflex latency of the paws of the rats were significantly decreased （P<0.05）， and the contents of NK， SP， TNF-α， and IL-1β in the dorsal root ganglia of the spinal cord and spinal cord of the rats were significantly increased （P<0.01）. Compared with the model group， the high-dose and medium-dose groups of topical administration of Jidesheng Sheyao tablets and the group of oral administration combined with topical application could significantly improve the lesions such as skin redness and herpes of the guinea pigs caused by VZV infection （P<0.01）， reduce the VZV viral nucleic acid load in the skin tissues of the guinea pigs （P<0.01）， alleviate the degree of inflammatory cell infiltration and skin cell necrosis in the skin tissue （P<0.05）， significantly increase the mechanical withdrawal threshold of the paws and the thermal withdrawal reflex latency of the paws of the rats （P<0.05）， and decrease the contents of NK， SP， TNF-α， and IL-1β in the dorsal root ganglia of the spinal cord and spinal cord of the rats （P<0.01）.ConclusionJidesheng Sheyao tablets demonstrated significant therapeutic effects on VZV-induced skin infections and postherpetic neuralgia （PHN）， providing a promising candidate for the prevention and treatment of VZV infections.

Schlüsselwort

Jidesheng Sheyao tablets;varicella-zoster virus;postherpetic neuralgia;dorsal root ganglion;mechanical withdrawal threshold of paw;thermal withdrawal latency

References

  1. 1.
    KENNEDY P G E，MOGENSEN T H，COHRS R J.Recent issues in Varicella-zoster virus latency［J］.Viruses，2021，13（10）：2018.
  2. 2.
    YOU H，REN H T，FAN S Y，et al.Clinical analysis of 7 cases of central nervous system involvement caused by varicella-zoster virus infection of the skin［J］.J Clin Dermatol，2024，53（8）：473-476.
  3. 3.
    WU S B，YANG S M，HUANG J B，et al.New progress in the research of animal models and mechanisms of postherpetic neuralgia ［J］.Chin J Pain Med，2023，29（2）：84-91.
  4. 4.
    GUO H，HU R P，MA Y H，et al.Pathogenic mechanism，prevention and treatment of varicella-zoster virus ［J］.Acta Acad Med Inner Mongolia，2021，43（2）：210-213，217.
  5. 5.
    KANNAN V，CHANDRAMOULEESWARAN V，BALASUBRAMANIAN S，et al.A study on neurological manifestations of primary varicella zoster virus infection［J］.Neurology Asia，2019，24（1）：9-14.
  6. 6.
    LI Y Z，JIN J L，KANG X H，et al.Identifying and evaluating biological markers of postherpetic neuralgia：A comprehensive review ［J］.Pain Ther，2024，13（5）：1095-1117.
  7. 7.
    YU S Y，WAN Y，WAN Q，et al.Chinese expert consensus on the diagnosis and treatment of postherpetic neuralgia［J］.Chin J Pain Med，2016，22（3）：161-167.
  8. 8.
    TOMMASI C，BREUER J.The biology of Varicella-zoster virus replication in the skin［J］.Viruses，2022，14（5）：982.
  9. 9.
    SHEN F，YANG D G，WU J，et al.Randomized controlled clinical study on the treatment of acute herpes zoster with valacyclovir hydrochloride tablets combined with jidesheng Sheyao tablets［J］.Shanghai Med Pharm J，2023，44（11）：3-6，16.
  10. 10.
    ZHAO W，SUN G Z.Dosage conversion among different kinds of experimental animals ［J］.Chin Anim Husb Vet Med Inf，2010，doi：.
  11. 11.
    GUO J，XUAN Z Y，CEN J，et al.Optimization of the extraction process of Xiaozhen Zhitong gel by combining pharmacodynamic experiments with orthogonal design［J］.Chin Tradit Herb Drugs，2021，52（14）：4210-4216.
  12. 12.
    LI Y，YOU Q，ZHAO X T，et al.Analysis of the clinical characteristics of 33 cases of nervous system involvement caused by cutaneous Varicella-zoster virus infection［J］.Chin J Nerv Ment Dis，2024，50（12）：705-710.
  13. 13.
    JIANG Y R.How does traditional Chinese medicine prevent the transmission of herpes zoster［N］.Gansu Science and Technology News，2024-08-19 （06）.
  14. 14.
    QIU J Z.The effect of acyclovir combined with glucocorticoids in the treatment on the pain and cytokine levels of patients with herpes zoster［J］.Mod Diagn Treat，2024，35（4）：527-529.
  15. 15.
    DU Z J，FENG Z Y.Progress in the treatment of herpes zoster neuralgia with integrated traditional Chinese and western medicine ［J］.Zhejiang J Integr Tradit Chin West Med，2024，34（11）：1077-1080.
  16. 16.
    GAO X L，LI Z R，YANG Y，et al.Study on the effect and mechanism of rosmarinic acid against Varicella-zoster virus in vitro［J］.J Chengdu Med Coll，2024，19（4）：563-567.
  17. 17.
    SUN Q Y，BAO L，GENG Z H，et al.Research on regulatory mechanism of verbenalin on HCoV-229E-infected macrophages injury based on mitophagy［J］.Chin J Exp Tradit Med Form，2025，doi：.
  18. 18.
    LI S R，WANG X W，SUN J，et al.Effect of Lutongning extract powder in treatment of trigeminal neuralgia induced by suborbital injection of talc into infraorbital foramen of model rats based on OTULIN-regulated neuroinflammation［J］.Chin J Exp Tradit Med Form，2025，doi：.
  19. 19.
    LI S R，WANG Y X，SUN J，et al.Exploring mechanism of Tibetan medicine Sanwei Doukou decoction to improve cognitive dysfunction in 5×FAD mice based on Wnt/β-catenin signaling pathway［J］.Chin J Exp Tradit Med Form，2025，doi：.
  20. 20.
    XIE D，LI S R，GENG Z H，et al.Establishment and application of animal models for disease-syndrome combination in viral pneumonia：A review［J］Chin J Exp Tradit Med Form，2025，doi：.
  21. 21.
    LI D H.Study on the regularities of formula-syndrome and medication in the treatment of herpes zoster with traditional Chinese medicine ［D］.Nanning：Guangxi University of Chinese Medicine，2015.
  22. 22.
    HUANG H L，LI Z H，LIU J J，et al.Exploration of the mechanism of action of polyphyllin Ⅰ from Paris polyphylla against cervical cancer based on network pharmacology and experimental verification ［J］.Drugs Clin，2025，40（2）：310-318.
  23. 23.
    WEI R N，ZHOU G C，ZUO K F.Overview of the modern clinical applications of paris polyphylla［J］.Henan Tradit Chin Med，2025，45（1）：157-162.
  24. 24.
    LI G Y，ZHANG J J，GU X Z，et al.Overview of the clinical application of toad skin ［J］.Chin J Exp Tradit Med Form，2014，20（14）：235-238.
  25. 25.
    HU Y，GAO B，ZHANG H，et al.Research progress on the processing history，chemical composition and pharmacological effects of scolopendra ［J］.Acta Chin Med Pharmacol，2024，52（6）：98-104.
  26. 26.
    CHEN D L，YAO S F，SUN W H，et al.Exploration of the pharmacodynamic substance basis and anti-inflammatory mechanism of euphorbia humifusa willd.by GC-MS，UPLC-Q-TOF-MS/MS combined with network pharmacology ［J］.J Hunan Univ Chin Med，2023，43（12）：2238-2248.
  27. 27.
    ZHANG L J.Efficacy observation of JI Desheng Sheyao tablet in the treatment of 58 cases of herpes zoster ［J］.J Med Theory Pract，1992，5（3）：24-25.
  28. 28.
    HUANG H D，TANG D M，ZHANG B R.Efficacy observation of a comparative study between JI Desheng Sheyao tablet and aciclovir tablets in the treatment of herpes zoster［J］.J Clin Dermatol，1996（3）：54.
  29. 29.
    GU D F.Treatment of 30 cases of herpes zoster with JI Desheng snake medicine ［J］.Guangxi J Tradit Chin Med，1994，16（1）：15-16.
  30. 30.
    HE J，YANG Z Q，LIU Y Y，et al.Pharmacodynamic study on the anti-herpes virus effect of JI Desheng Sheyao tablet in vitro ［J］.J Shandong Univ Tradit Chin Med，2006（3）：258-259.
  31. 31.
    LI B F，LIU B，WANG C M，et al.Effect of pulsed radiofrequency combined with nerve blockade therapy on SF-MPQ score and serum levels of substance P and IL-1β in patients with refractory postherpetic neuralgia ［J］.J Inter Radiol，2024，33（11）：1197-1202.
  32. 32.
    LI Y Z，JIN J L，KANG X H，et al.Identifying and evaluating biological markers of postherpetic neuralgia：A comprehensive review ［J］.Pain Ther，2024，13（5）：1095-1117.
  33. 33.
    LIU X B，MA Y C，YANG B.Efficacy of modified Shaoyao Gancao decoction combined with auricular point pressing beans in the treatment of postherpetic neuralgia ［J］.Chin J Gerontol，2025，45（5）：1117-1121.
  34. 34.
    KANG L，ZHANG M，YI H L，et al.Clinical observation of traditional Chinese medicine decoction combined with fire needling in the treatment of patients with postherpetic neuralgia ［J］.World J Integr Tradit West Med，2025，20（2）：314-319.
  35. 35.
    XIE X T，NIU F Q，PAN Y J，et al.Research status of traditional chinese medicine in regulating TRPV1 signaling pathway and related factors for the prevention and treatment of herpes zoster and postherpetic neuralgia［J］.Chin J Clin Pharm，2025，41（3）：433-437.
  36. 36.
    LI L N，GONG X R，XIAO S G，et al.Clinical efficacy of local drug injection combined with pulsed radiofrequency in the treatment of postherpetic neuralgia［J］.Med J West China，2025，37（8）：1211-1215.
  37. 37.
    YUAN F，FENG D，LI S J，et al.Specific cellular immunity against Varicella-zoster virus and its value in predicting postherpetic neuralgia［J］.J Pract Med，2023，39（14）：1809-1814.
  38. 38.
    JING J Y，ZHAO Z，ZHANG H B，et al.Pathogenesis of inflammatory cytokines in herpes zoster and research progress in traditional Chinese medicine［J］.Chin Arch Tradit Chin Med，2025，doi：.
  39. 39.
    HU J，YANG C J，HE X S，et al.Effects and mechanisms of JI Desheng Sheyao tablet on local and systemic inflammatory responses at the wound sites of snakebite patients［J］.China Pharm，2021，32（17）：2102-2107.
  40. 40.
    ZHANG N，ZHANG H J，FAN Y Y.Mechanistic study of JI Desheng Sheyao tablet in the treatment of acute gouty arthritis in rats［J］.Anhui Med Pharm J，2025，29（2）：253-257，425.
  41. 41.
    LI C H.Preliminary study on the effects of total saponins of Paris polyphylla var.yunnanensis on rats with non-alcoholic steatohepatitis and its NF-κB signaling pathway［D］.Kunming：Yunnan University of Traditional Chinese Medicine，2021.
  42. 42.
    TIAN Y，GONG G Y，MA L L，et al.Anti-cancer effects of polyphyllinⅠ：An update in 5 years ［J］.Chem Biol Interact，2020，316：108936.
  43. 43.
    LONG L N，ZHONG W W，GUO W L，et al.Effect of Bufalin-PLGA microspheres in the alleviation of neuropathic pain via the CCI model ［J］.Front Pharmacol，2022，13：910885.
  44. 44.
    FANG X T，MO K Y.Research progress on the pharmacology of Scolopendra ［J］.Guide China Med，2015，13（18）：32-34.
  45. 45.
    CHEN J，XIE J H，HAN Y Q，et al.Anti-inflammatory and antioxidant effects of the aqueous extract of Euphorbia humifusa Willd. in IPEC-J2 cells［J］.China Anim Husb Vet Med，2023，50（2）：704-712.

Lesen Sie die ganze Passage

The above content is generated by Large Model Translation. The translated content is for reference only. We do not assume any commercial or legal responsibilty for any consequences arising from the use of our website