最新刊期
卷 24 ， 期 11 ， 2018
- 摘要：Objective: To elucidate the compatibility mechanism of Mahuang Xixin Fuzitang (MXF) for external use and accumulate data for the study of compatibility mechanism of Chinese herbal prescription for external use,the influence on in vitro skin permeation profiles of MXF composed by different prescription was investigated in this paper. Method: The rat back percutaneous permeation studies of Mahuang group,Fuzi group,Xixin group,Mahuang Fuzi group,Mahuang Xixin group,Fuzi Xixin group,Mahuang Fuzi Xixin group (the whole prescription group) were carried out to compare the permeation effect of different prescriptions by in vitro Franz diffusion cell method,taking normal saline solution containing 1% polysorbate-80 (tween-80) as the receiving solution,cumulative permeation quantity in 12 h (Q12 h) and steady penetration rate (Jss) of ephedrine hydrochloride,pseudoephedrine hydrochloride,benzoylmesaconine,benzoylhypaconine,benzoylaconine,methyleugenol and asarinin as indexes. Result: Comparison of Q12 h and Jss of various components,the rank order of ephedrine hydrochloride or pseudoephedrine hydrochloride was whole prescription group > Mahuang Xixin group > Mahuang group > Mahuang Fuzi group,and ephedrine hydrochloride > pseudoephedrine hydrochloride;benzoylmesaconine,benzoylhypaconine and benzoylaconine were in the order of Fuzi Xixin group>Fuzi group>Mahuang Fuzi group > whole prescription group,and benzoylmesaconine > benzoylaconine > benzoylhypaconine;methyleugenol was ranked as Xixin group > Mahuang Xixin group > Fuzi Xixin group > whole prescription group;the difference of Q12 h and Jss of asarinin among groups was small. Conclusion: The percutaneous permeation of all kinds of components in MXF can be influenced by different compatibility,the impact was different according to different component.Asari Radix et Rhizoma can promote the percutaneous permeation of ephedrine hydrochloride,pseudoephedrine hydrochloride,benzoylmesaconine,benzoylhypaconine and benzoylaconine;Aconiti Lateralis Radix Praeparata can inhibit the percutaneous permeation of ephedrine hydrochloride and pseudoephedrine hydrochloride;both of Ephedrae Herba and Aconiti Lateralis Radix Praeparata can significantly inhibit the percutaneous permeation of methyleugenol.关键词：Mahuang Xixin Fuzitang;warm channel and expelling cold;compatibility;percutaneous permeability characteristics;decomposed recipes;alkaloids;volatile oil6|6|1更新时间：2024-01-04
- 摘要：Objective: To analyze the petroleum ether extracts in water decoction of Curcumae Radix pieces from four different sources,and find out the common and different components. Method: GC-MS was adopted to determine the petroleum ether extracts with carrier gas of helium,temperature was risen by program,ionization mode was electron bombardment ion source. Result: Fifty components were determined in the petroleum ether extracts in water decoction of Curcumae Radix pieces from four different sources,and 3 common components were found in four Curcumae Radix pieces;9 common components were found in three Curcumae Radix pieces;25 common components were found in two Curcumae Radix pieces;37 components were found in two or more Curcumae Radix pieces,accounting for 74% of total number of detected components. Conclusion: Curcumae Radix pieces from four different sources have common and different components in the petroleum ether extracts of water decoction,and the common and cross-existent components may be one of the material basis for using as the same medicine in the clinic.关键词：Curcumae Radix;pieces;water decoction;petroleum ether extracts;gas chromatography-mass spectrometry;vanillin;dibutylphthalate6|6|4更新时间：2024-01-04
- 摘要：Objective: To prepare self-microemulsion of total valepotriates in Valerianae Jatamansi Rhizoma et Radix and evaluate its quality. Method: The prescription was selected by solubility experiment,compatibility test and ternary phase diagram method.Taking rates of oil phase,emulsifier and co-emulsifier as factors,the particle size,self-emulsifying time and drug loading as indexes,simplex centroid mixture design was applied to optimize the prescription of self-microemulsion of total valepotriates in Valerianae Jatamansi Rhizoma et Radix,and the pharmaceutical properties of the self-microemulsion were evaluated. Result: Optimum formulation of the self-microemulsion was 10% of ethyl oleate,61% of polyoxyl 15 hydroxystearate,29% of diethylene glycol monoethyl ether;its average particle size was 38.8 nm,self-emulsifying time was 0.57 min,and drug loading was 131.23 mg·g-1 in the optimal prescription,cumulative dissolution at 5 min was 94.99%.Self-microemulsion of total valepotriates in Valerianae Jatamansi Rhizoma et Radix was affected by high temperature and illumination,indicating it should be stored at low temperature and avoid light. Conclusion: The preparation technology of this self-microemulsion is simple,it can significantly improve the solubility,bioavailability and dissolution of total valepotriates in Valerianae Jatamansi Rhizoma et Radix.关键词：Valerianae Jatamansi Rhizoma et Radix;total valepotriates;antidepressant;sedative-hypnotic;self-microemulsion;ternary phase diagram;simplex centroid mixture design8|6|4更新时间：2024-01-04
- 摘要：Objective: To explain the effect of water grinding,water washing and grinding on the quality of Realgar by comparing the contents of As2S2 and elements in different processed products.At the same time,to identify water grinding products,water washing products and grinding products rapidly. Method: To determine the content of As2S2 in different processed products of Realgar by the method in the 2015 edition of Chinese Pharmacopoeia.Scanning electron microscopy-energy dispersive spectrometer (SEM-EDS) was used to observe the surface morphology of different processed products and determine the contents of elements in different processed products. Result: The content of As2S2 in different processed products ranked as water grinding products > water washing products > grinding products.The surface morphology of water grinding products was similar to the surface morphology of water washing products,but the surface morphology of grinding products was different from theirs.The content of oxygen in different processed products showed the trend of grinding products > water washing products > water grinding products. Conclusion: It can be distinguished between water grinding products and grinding products by SEM-EDS,it provides a certain technical support for the identification of different processed products of Realgar.The effect of water grinding on removing impurities and reducing the toxicity is best,quality of water grinding products is better than the quality of water washing products and grinding products,which shows that water grinding for Realgar has scientific basis.关键词：Realgar;water grinding;water washing product;grinding product;scanning electron microscopy-energy dispersive spectrometer10|6|2更新时间：2024-01-04
- 摘要：Objective: To isolate and purify polysaccharides in Glehniae Radix (GRP) and investigate the structural characterization and in vitro immunological activity of each purified fraction (GRP-1,GRP-2 and GRP-3). Method: GRP was obtained by water extraction and ethanol precipitation method,and then GRP was subjected to protein removal by Sevage method,and then underwent further purification by DEAE cellulose DE-52 column chromatography and Sephadex G-75 column chromatography.The content of GRP and its monosaccharide composition were determined by ultraviolet spectrophotometry (UV) and high performance liquid chromatography combined with derivatization of 1-phenyl-3-methyl-5-pyrazolone (HPLC-PMP),molecular weight and structural characterization of GRP-1 were carried out by infrared spectrometer (IR) and multi-angle laser light scattering instrument,respectively.The viability of mouse spleen lymphocytes treated with the three fractions was determined by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. Result: Three fractions of GRP-1,GRP-2 and GRP-3 were obtained after separation and purification,GRP-1 with a relative molecular weight of 23.01 kDa was a homogeneous polysaccharide,while GRP-2 and GRP-3 were two hetero polysaccharides.The contents of polysaccharides in the three fractions were 96.16%,48.64% and 89.73%,respectively.GRP-1 was composed of mannose,glucuronic acid,rhamnose,glucose,galactose and arabinose in a molar ratio of 81.86∶0.12∶0.17∶1 259.7∶0.54∶0.33.Structure of GRP-1 might have α-glycosidic bond.In the end,in vitro immunological activity results showed that the three polysaccharide components exhibited good immune activity. Conclusion: GRP-1 is a homogeneous polysaccharide with strong immunological activity in vitro.关键词：Glehniae Radix;polysaccharides;isolation;purification;immune activity;anti-tumor;infrared spectroscopy6|7|8更新时间：2024-01-04
- 摘要：Objective: To clone the cDNA sequence of chalcone synthase (CHS) in Glycyrrhiza glabra and analyse its genetic polymorphism. Method: The cDNA sequence of CHS was cloned from root of G. glabra by reverse transcription polymerase chain reaction (RT-PCR),then sequenced and analysed by bioinformatics software. Result: A total of 19 cDNA sequences of CHS with the full length of 1 175 bp were obtained from G. glabra samples,these 19 sequences with eighty-one polymorphic sites can be divided into 17 haplotypes,the similarity of which was 99.10%.Amino acid sequence analysis indicated that these 19 cDNA sequences encoded 14 amino acid sequences,the similarity of which was 99.34%,and 25 variable sites were determined.Bioinformatics analysis has shown that CHS was a stable hydrophilic protein,its average relative molecular weight was 42.6 kDa and isoelectric point was 5.75-6.21,it contained no signal peptides or transmembrane domains and had a conversed domain of chalcone synthase superfamily,its secondary structure mainly constituted of α-helix and random coil.The homologoue analysis showed that CHS cDNA sequence of G. glabra had the closest relationship to G. uralensis and G. inflata,and the furthest relationship to Physcomitrella patens. Conclusion: In this study,CHS cDNA sequence is successfully cloned from G. glabra samples and it shows abundant polymorphism.关键词：Glycyrrhiza glabra;chalcone synthase;cloning;bioinformatics;Physcomitrella patens;G. uralensis;G. inflata7|5|1更新时间：2024-01-04
- 摘要：Objective: To research the Araliaceae plant of Panax notoginseng flower,P. ginseng flower and P. quinquefolius flower by identifying their mini-characters, and provide scientific basis for the identification of mini-characters of this kind of medicinal materials. Method: Traditional Chinese medicine micro-macroscopical identification method was used to collect the information of the samples sold on the market by electronic eyepiece. Meanwhile,mini-characters of the P. notoginseng flower,P. ginseng flower and P. quinquefolius flower were identified by synthetic technique of Photoshop CS6 software. Then its surface mico-morphological characteristics were described, and finally,the key identification points were summed up. Result: The main distinguishing points of micro-trait included surface of peduncle,pedicel and pistil. The surface of the peduncle and pedicel of P. notoginseng flower had non-glandular hair and its pedicel surface white protrusion was arranged irregularly, and the separated P. notoginseng flower pistil stigma had brown yellow resin channel. P. ginseng flower peduncle and pedicel surface had no non-glandular hair and its frequently unseparated pistil stigma had unobvious yellow resin channel. The surface of the peduncle and pedicel of P. quinquefolius flower had non-glandular hair and its pedicel surface white protrusion was arranged regularly. The unseparated P. quinquefolius flower pistil stigma had no obvious resin channel. Conclusion: Through the micro-morphological identification method,we can clearly observe the surface features of them and the P. notoginseng flower,P. ginseng flower,P. quinquefolius flower can be distinguished effectively,providing the basis for the identification and quality evaluation of the P. ginseng flower.关键词：Panax notoginseng flower;P. ginseng flower;P. quinquefolius flower;mico-morphological identification;anti-hypertension6|7|3更新时间：2024-01-04
- 摘要：Objective: Fingerprint and anti-tumor pharmacodynamic analysis were used to probe the similarity of the principal pharmacodynamic components of Arisaema peninsulae and A. amurense,providing data support for the rational clinical use of A. peninsulae. Method: High performance liquid chromatography (HPLC) method was used to establish the fingerprint of 11 batches of A. peninsulae and A. amurense from different origins or harvest time points. Thiazolyl blue(MTT) assay was used to investigate their inhibition effect on SMMC-7721 cells proliferation. Grey relational analysis and partial least-squares regression analysis methods were used to analyze the spectrum-activity relationship of 6 batches of A. peninsulae and 5 batches of A. amurense respectively, aimed to find the ingredients with greater contribution to efficacy and evaluate the similarities. Result: The fingerprint of 11 batches of A. peninsulae and A. amurense from different origins or harvest time points was established, and their anti-tumor activity was verified. Grey relational analysis results showed that 12 components from 15 components of A. peninsulae whose pharmacodynamic correlation coefficient was greater than 0.80 were the same as A. amurense. And the rationality of the grey relational analysis results was verified by partial least-squares regression analysis. The comprehensive analysis proved that A. peninsulae had a good similarity with A. amurense based on the main pharmacodynamic components. Conclusion: Guided by the anti-tumor efficacy,this experiment verified the similarity of chemical compositions between A. peninsulae and A. amurense,providing reasonable basis for the use of A. peninsulae,and providing experimental basis for its quality control.关键词：Arisaema peninsulae;A. amurense;anti-tumor;spectrum-activity relationship;similarity evaluation6|6|5更新时间：2024-01-04
- 摘要：Objective: To investigate the chemical constituents of Solanum septemlobum. Method: The ethyl acetate fraction of 95% ethanol extract of S. septemlobum was isolated and purified by silica gel column chromatography,Sephadex LH-20 and high performance thin layer chromatography (HPTLC). The structures were identified by physico-chemical properties and spectral data. Result: Twenty-one compounds were isolated and purified as: N-trans-coumaroyltyramine(1),N-trans-feruloyl-4'-O-methyldopamine(2),p-hydroxybenzoic acid (3),3,4-dimethoxy benzoic acid(4),protocatechuate (5),methyl 3,4-dihydroxybenzoate(6),2-hydroxybenzoic acid(7),ethyl 4-hydroxybenzoate(8),ethyl 3,4-dihydroxybenzoate(9),esculetin(10),liquiritigenin(11),hesperetin(12),naringenin(13),luteolin(14), apigenin(15),hrysoeriol(16),diosmetin(17),5,7-dihydroxy-3',4'-dimethoxyflavone(18),isoliavoues(19), genistein(20),and N-trans-feruloyltyramine(21). Conclusion: Compounds 1-20 were isolated from S. septemlobum and compounds 3-12 and 14-17 were obtained from Solanum for the first time. The literature review showed that amides and flavonoids had anti-inflammatory and antioxidant activities,providing a reference for further studies on pharmacodynamic material basis and the development and utilization of S. septemlobum from Xinjiang.关键词：Solanaceae;Solanum;Solanum septemlobum from Xinjiang;chemical constituent;clearing heat and removing toxicity7|6|0更新时间：2024-01-04
- 摘要：Objective: To identify the origins of Gastrodiae Rhizoma by nonlinear chemical fingerprint technology and HPLC, make a quantitative analysis of gastrodin and global active components in Gastrodiae Rhizoma. Method: Gastrodiae Rhizoma from Yunnan, Hubei, Guizhou and Northeast China were collected and identified by nonlinear fingerprint and HPLC fingerprint. With Gastrodiae Rhizoma sample as oscillation substrate, H2SO4+MnSO4+NaBrO3+CH3COCH3+sample compositions was taken as the oscillation system to establish the nonlinear chemical fingerprint of Gastrodiae Rhizoma. Based on the significant differences of the nonlinear fingerprint and system similarity model, Gastrodiae Rhizoma from different areas was identified successfully, and their global active components were detected. Meanwhile, HPLC fingerprints of Gastrodiae Rhizoma from 4 different origins were established. Then a fuzzy cluster analysis was made to study their quality difference, and gastrodin was detected. Result: The classification results by nonlinear chemical fingerprint and HPLC fingerprint were consistent, and can trace to the origins of Gastrodiae Rhizoma. The relative contents of functional components in Gastrodiae Rhizoma produced from different regions showed different quality. HPLC fingerprint and non-linear chemical fingerprint were combined to detect each single active component and obtain the result of global content of active components. Conclusion: This study provides a new economical and rapid method for identification of origins and quality evaluation of Gastrodiae Rhizoma and new ideas to represent active components in Gastrodiae Rhizoma.关键词：nonlinear chemical fingerprint;HPLC;Gastrodiae Rhizoma;origin identification;gastrodin;global active component7|6|1更新时间：2024-01-04
- 摘要：Objective: To study on the volatile oil components in herbal pair(HP) of Atractylodis Macrocephalae Rhizoma(AMK)-Poria(PCW) and its single herb,and to investigate the different HP proportions on the the volatile oil. Method: Volatile oil was extracted from HP and its single herb by organic-steam distillation. The chemical constituents were analysised and identified by GC-MS technique and combined with mass spectrometry library searching and literature comparison. Result: There were 26,22,30,29 and 41 volatile constituents in AMK,PCW,HP(1∶1),HP(1∶2) and HP(2∶1), and 3 common volatile constituents were identified. There were linoleic acid,palmitic acid and α-cedrene. 16 compounds were identified from volatile oil of AMK and the three HP. The top three were (4aS,8aR)-3,8a-dimethyl-5-methylene-4,4a,5,6,7,8,8a,9-octahydronaphtho[2,3-b]furan,(-)-g-elemene,β-panasinsene. And volatile oil of PCW were only six ingredients can be to detect from the three HP,accounts for only 27% of PCW. Conclusion: Regarding the volatile oil components and content,the volatile chemical components in the three HP were mainly from single herb AMK,the volatile oil components of different HP proportions have different degrees of change and the most obvious one is the HP(2∶1). This may be associated with the increased dosage of AMK. And all the three HP have new components detected. The reason may be due to the occurrence of certain physical changes or chemical reactions during the decocting process.关键词：Atractylodis Macrocephalae Rhizoma;Poria;paired drugs;different proportion;volatile oils;gas chromatography-mass spectrometry6|7|2更新时间：2024-01-04
- 摘要：Objective: To observe the effect of Shenling Baizhu San on the expressions of interleukin (IL)-13, IL-23 and cyclooxygenase-2 (COX-2), CAMP response element binding protein (CREB) in ulcerative colitis rats with spleen deficiency and dampness, in order to explore its mechanism. Method: Totally 48 SPF Wistar rats were randomly divided into 6 groups: blank group, model group, high, medium and low-dose Shenling Baizhu San groups, and mesalazine group, with 8 rats in each group, half male and half female. Except for blank group, the rats in the other groups were included into the saphenous ulcerative colitis model by the combination of disease and syndrome, including diet, environmental intervention and 2,4-dinitrobenzene sulfonic acid enema. After successful modeling, Shenling Baizhu San group was respectively treated with the drug at high dose, middle dose and low dose (24, 12, 6 g·kg-1·d-1), and mesalazine group was treated with the drug (0.2 g·kg-1·d-1) for gavage. After 21 days of treatment, the levels of IL-13 and IL-23 in colonic tissues were detected by enzyme-linked immunosorbent assay (ELISA); mRNA expressions of IL-13, IL-23, COX-2, CREB in colonic tissues were detected by Real-time PCR; the protein expressions of COX-2, CREB in colonic tissues were detected by Western blot. Result: Compared with the blank group, the model group rats' IL-13 content and IL-13 mRNA expression in colon were significantly decreased (P<0.05), IL-23 content and IL-23 mRNA expression increased (P<0.05), COX-2, CREB mRNA expression and relative protein expression increased (P<0.05). Compared with the model group, IL-13 and IL-13 mRNA expressions in the colonic group were significantly higher than the rats treated with Shenling Baizhu San (high, medium and low doses), especially in the high-dose group (P<0.05), IL-23 content and IL-23 mRNA expression decreased (P<0.05), and COX-2, CREB mRNA expressions and relative protein expression decreased (P<0.05). Conclusion: Shenling Baizhu San can strengthen spleen Qi and excrete moisture, which may regulate the expressions of IL-13, IL-23 and COX-2,CREB in the colon of rats with spleen deficiency and dampness, so as to treat colonic ulcer.关键词：Shenling Baizhu San;ulcerative colitis;interleukin-13 (IL-13);interleukin-23 (IL-23);cyclooxygenase-2 (COX-2);CAMP response element binding protein (CREB)9|6|9更新时间：2024-01-04
- 摘要：Objective: To investigate the protective effect of Tanreqing injection and its five intermediates on carbon tetrachloride (CCl4)-induced acute liver injury in rats and preliminarily discuss its pharmacodynamical basis against acute liver injury. Method: Subcutaneous injection was given for several times to establish CCl4-induced acute liver injury models. Then the male rats were randomly divided into normal group, CCl4 model group, Tanreqing injection group, Tanreqing injection treatment group, diammonium glycyrrhizinate injection group, Scutellariae Radix group, Selenarcti Fel group, Caprae Hircus Cornu group, Forsythiae Fructus group and Lonicerae Japonicae Flos group. The drugs were given to experimental rats for seven days by tail intravenous injection. Rats’ body weight, liver/body weight ratio, serum biochemical analyses and histological assays were measured. Quantitative real-time fluorescent PCR (Real-time PCR) was employed to detect the mRNA levels of Na+-dependent taurocholate co-transporting polypeptide (NTCP), sulfotransferase 2A1 (SULT2A1), glutathione-S transferase A2 (GSTA2) and cytochrome P450 3A11 (CYP3A11). Result: As compared with the normal group, the body weight was significantly decreased, liver/body weight ratio was significantly increased, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (T-Bil), direct bilirubin (D-Bil) and total bile acid (TBA) were also significantly increased, with significant necrosis and denaturation of hepatocytes, and the mRNA expression levels of NTCP, GSTA2 and CYP3A11 were significantly reduced in CCl4 model group (P<0.05, P<0.01). As compared with the model group, Tanreqing injection could significantly restore liver coefficient, reduce serum biochemical indexes, and alleviate pathological inflammation of liver tissues. Moreover, the improvement effect of the intermediates on serum liver function indexes was showed as Forsythiae Fructus>Lonicerae Japonicae Flos>Caprae Hircus Cornu>Selenarcti Fel. As compared with the model group, the mRNA expression levels of NTCP, GSTA2 and CYP3A11 were markedly up-regulated in Tanreqing injection group and Forsythiae Fructus group; the mRNA levels of SULT2A1 and GSTA2 were significantly up-regulated in Selenarcti Fel group; and the mRNA levels of SULT2A1, GSTA2 and CYP3A11 were up-regulated in Lonicerae Japonicae Flos group (P<0.05, P<0.01). Conclusion: Tanreqing injection exerts a potent protective effect against CCl4-induced hepatic injury, and the possible pharmacodynamical parts of this activity likely included Selenarcti Fel, Caprae Hircus Cornu, Forsythiae Fructus and Lonicerae Japonicae Flos. The possible mechanism of this activity may be related to up-regulating the mRNA expressions of NTCP, SULT2A1, GSTA2 and CYP3A11 in liver and accelerating the metabolism of bile acids in hepatocytes.关键词：Tanreqing injection;carbon tetrachloride;acute liver injury;pharmacodynamical basis6|6|6更新时间：2024-01-04
- 摘要：Objective: To explore the regulatory effect and mechanism of Astragali Radix-Puerariae Lobatae Radix compatibility on glucose-lipid and inflammation in diabetic rats and analyze their interaction in glucose and lipid lowering and anti-inflammatory process. Method: Total 66 rats were randomly divided into 6 groups. Except the normal group, diabetes model was induced by intraperitoneal injection of streptozotocin (STZ) in other groups. Distilled water (10 mL·kg-1) was given in normal group and model group;Jinqi Jiangtangpian suspension (1.47 g·kg-1) was given in positive control group;Astragali Radix (2.7 g·kg-1),Puerariae Lobatae Radix (1.35 g·kg-1), and Astragali Radix-Puerariae Lobatae Radix decoction (4.05 g·kg-1) were given respectively in corresponding treatment groups. After 30 days treatment, the rats were sacrificed. The contents of insulin (Ins), insulin receptor (InsR) and tumor necrosis factor α (TNF-α) in rats liver tissue were detected by enzyme-linked immunosorbent assay (ELISA). Real-time PCR was used to detect adiponectin receptors 1 (AdipoR1), AMP-activated protein kinase (AMPK), glucose transporter-4 (GLUT-4), peroxisome proliferator-activated receptor α (PPARα) and fatty acid transport protein 4 (FATP4), and p38 mitogen-activated protein kinase (p38 MAPK) mRNA expression in rat liver tissues(P<0.05). Result: Astragali Radix, Puerariae Lobatae Radix and their compatibility increased the level of Ins (except Puerariae Lobatae Radix) and InsR, rised AdipoR1, AMPK(except Puerariae Lobatae Radix), GLUT-4, PPARα and FATP4 mRNA, lowered TNF-α(except Puerariae Lobatae Radix) and p38 MAPK mRNA expression in liver tissues of diabetes rats.In regulating InsR,PPARα mRNA and TNF-α, Astragali Radix showed better effect than the compatibility of Astragali Radix and Puerariae Lobatae Radix. Conclusion: Astragali Radix-Puerariae Lobatae Radix compatibility presented particular emphasis on lowering glucose-lipid and anti-inflammatory, and the mechanism may be associated with increasing the liver Ins, GLUT-4, FATP4 mRNA and lowering p38 MAPK mRNA levels.关键词：compatibility of Astragali Radix and Puerariae Lobatae Radix;lowering glucose;lowering lipid;anti-inflammation;mechanism6|7|9更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of Kelong capsule on testosterone-induced benign prostatic hyperplasia (BPH) and its antioxidant mechanism in rats. Method: Forty male SD rats were randomly divided into normal group, model group, low-dose Kelong capsule group (3.6 g·kg-1), high-dose Kelong capsule group (7.2 g·kg-1) and nuclear factor E2-related factor 2(Nrf2) intervention group, n=8 in each group. Except the rats in normal group, all the other rats received subcutaneous injections of testosterone propionate (TP) to establish BPH models. Drugs were concomitantly administered by oral gavage for 4 weeks. Then the effects of different drugs on wet weight of the prostate and prostate index (PI) were observed, and haematoxylin-eosin (HE) staining was used to observe the morphological changes. The malondialdehyde (MDA) and glutathione (GSH) contents in serum were detected by enzyme-linked immunosorbent assay(ELISA) kit. The mRNA levels of Nrf2, oxygenase-1 (HO-1) and quinone oxidoreductase (NQO1) were determined with real time fluorescent quantitative polymerase chain reaction (Real-time PCR). Result: As compared with the normal group, the wet weight of prostate and PI were increased and the prostatic epithelial was proliferated in the model group; GSH content in serum was significantly reduced; and mRNA expression levels of Nrf2, HO-1 and NQO1 were also significantly reduced (P<0.05, P<0.01). As compared with the model group, Kelong capsule significantly decreased prostate index, ameliorated the histological changes, increased the content of GSH in serum(P<0.01), and significantly up-regulated Nrf2, HO-1 and NQO1 mRNA levels (P<0.05,P<0.01). Conclusion: Kelong capsule could effectively inhibit the development of BPH, and low dose Kelong capsule could activate Nrf2/ARE signal pathway to increase the antioxidant capacity of the body.关键词：Kelong capsule;benign prostatic hyperplasia;nuclear factor-E2-related factor 2(Nrf2);oxidative damage9|6|3更新时间：2024-01-04
- 摘要：Objective: To observe the effects of Qingzao Jiufei Tang (QJT) on the ultrastructure and the expression of cytotoxin MPN372, aquaporin 5 (AQP5) and pulmonary surfactant related protein-A (SP-A) in lung tissue of mice infected with mycoplasma pneumoniae (MP), and explore its molecular mechanism on ‘moistening dryness’. Method: Totally 96 SPF BABL/c mice were randomly divided into normal group, model group, QJT group (0.3 mL/mice) and Azithromycin group (0.3 mL/mice), n=24 in each group. Except the normal group, the the mice in other 3 groups were induced into MP infection models. On day 3, 7, 10, 14 after modeling, the degree of pathological inflammation in lung tissue of mice was scored; the lung index and ratio of wet and dry was calculated; the ultrastructure of lung tissue was observed by electron microscope; the mRNA expression levels of MPN372 and SP-A in lung tissue were detected by real time fluorescence quantitative polymerase chain reaction(Real-time PCR) and the protein expression of AQP5 was detected by immunohistochemical method and Western blot method. Result: After MP infection, lung pathology score and lung index were increased; the ratio of dry and wet was reduced, and the inflammation was the most serious on day 7 as compared with the normal group; on day 7, microvilli fell off, mitochondria swollen, and alveolar septum thickening could be observed by electron microscope; the mRNA expression of MPN372 in lung tissue of mice was increased and reached the peak on the seventh day, while AQP5 protein expression and the SP-A mRNA expression were decreased and reached the valley on the seventh day (P<0.05). As compared with the model group, the inflammation of lung tissue could be controlled; the inflammatory changes under the electron microscope were relieved; the mRNA expression of MPN372 was decreased, AQP5 protein expression and SP-A mRNA expression were increased after treatment with QJT (P<0.05); SP-A and AQP5 expression levels were significantly different between QJT group and Azithromycin group on day 14 (P<0.05). Conclusion: QJT can embody the effect of “moistening dryness” by reducing the inflammation of the lung, reducing the content of the toxin and increasing the content of the active substance in the lung tissue.关键词：Qingzao Jiufei Tang;moistening dryness;mycoplasma pneumoniae;aquaporin;pulmonary surfactant6|6|5更新时间：2024-01-04
- 摘要：Objective: To establish a gas chromatography (GC) method for comparison of drug concentration in plasma of caffeine in normal and diabetic cognitive dysfunction rats after intragastric administration of green tea extract,and further validate its influence on diabetic cognitive dysfunction. Method: Diabete models were copied by one-time intraperitoneal injection streptozotocin (STZ) with dose of 50 mg·kg-1,after successful replication of the model,diabetic rats were intervened by gavage of caffeine and green tea extract,respectively;blood glucose value was determined once every three weeks,cognitive function was analyzed with Morris water maze experiment at the 12 week,and then GC was used to study on pharmacokinetics of caffeine in normal and diabetic cognitive dysfunction rats,and transmission electron microscopy was employed to observe ultrastructure of neurons. Result: The linear range of caffeine was 1.28-128 mg·L-1(R2=0.998),the minimum test limit and the minimum quantitative limit were 0.7,1.28 mg·L-1,respectively.The RSD of inter-day and intra-day precision were less than 8.0%,the average method and extraction recoveries were more than 90%.The area under the curve (AUC0-t) of caffeine in normal and diabetic cognitive dysfunction rats were(46.71±4.25),(61.2±5.44) mg·h·L-1,the half-life (t1/2) were (2.82±0.38),(3.71±0.13) h,the peak plasma concentration (Cmax) were (13.25±2.02),(17.12±1.08) mg·L-1,the clearance (CL) were (0.43±0.08),(0.33±0.07) L·kg-1·h-1,respectively.Compared with the model group,the blood glucose of caffeine group and green tea extract group dropped,their cognitive ability and ultrastructure of neurons were improved. Conclusion: Metabolism of caffeine in normal and diabetic cognitive dysfunction rats has difference,and it can improve the diabetic cognitive dysfunction.This study provides a theoretical basis for clinical use of green tea and caffeine in preventing diabetic cognitive dysfunction.关键词：green tea;caffeine;diabetic cognitive dysfunction;pharmacokinetics;pharmacodynamics;pseudoephedrine hydrochloride;blood-brain barrier7|6|1更新时间：2024-01-04
- 摘要：Objective: To study the anti-proliferative and apoptotic effects of the combined application of casticin (CAS) and (-)-Epigallocatechin gallate(EGCG) on A549 lung cancer cells. Method: A549 lung adenocarcinoma cells were incubated, and those with the activity of at least 95% during logarithmic phase were assayed. Next, the anti-proliferative effect of the combined application of CAS (0.5, 1, 5, 10, 15, 20 μmoL·L-1) and EGCG (1, 5, 10, 15, 20, 25 μmoL·L-1) on A549 cells was detected by sulforhodamine B (SRB) method. After that, the intracellular distribution of CAS and EGCG was observed under confocal laser scanning microscope. Flow cytometry was used to detect the apoptosis of A549 lung cancer cells. Protein expressions of B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein (Bax) in A549 cells were detected by Western blot. Result: The inhibitory rates of these two drugs with different concentrations against A549 cells were statistically different (P<0.05) at 24, 48 h. Additionally, the inhibitory rates of the combined application of CAS+EGCG were significantly higher than the single application of CAS or EGCG with the same concentration(P<0.05). When the cells were incubated for 24 hours after the treatment with 5 μmoL·L-1CAS and 10 μmoL·L-1 EGCG, the drugs showed an anti-proliferative effect on A549 cells with synergy. Moreover, at 48 hour after the treatment, the combined application of 0.5 μmoL·L-1 CAS and 1 μmoL·L-1 EGCG, 1 μmoL·L-1 CAS and 5 μmoL·L-1 EGCG, 15 μmoL·L-1 CAS and 20 μmoL·L-1EGCG, as well as 20 μmoL·L-1CAS and 25 μmoL·L-1 EGCG showed an increased effect on the inhibitory rates on A549 adenocarcinoma cells. According to the observation under laser confocal microscope, the number of the group of CAS (5 μmoL·L-1) and EGCG (10 μmoL·L-1) entering A549 cells was obviously higher than the single application of CAS or EGCG with the same concentration. Meanwhile, after the cells were treated for 24 h, the apoptotic rate of the combined application of CAS and EGCG were obviously higher than that of the single application of CAS or EGCG with the same concentration (P<0.05). The expressions of Bax protein of EGCG were obviously higher than that of the single application of CAS or EGCG with the same concentration, while the expression of Bcl-2 was lower than that of the single application of CAS or EGCG with the same concentration (P<0.05). Conclusion: The inhibitory and apoptotic effects of co-administrated with CAS and EGCG on A549 lung cancer cells were significantly enhanced compared with those of the single administration, which may be related to Bcl-2 and Bax proteins.关键词：casticin;(-)-Epigallocatechin gallate;A549 lung cancer cell;apoptosis;anti-proliferation6|6|1更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of aloe-emodin on the growth, migration and fibrous actin (F-actin) of hepatoma HepG2 cells. Method: The maximum toxic concentration (TC0) of aloe-emodin on human normal hepatocytes was screened out in the drug toxicity experiment for human normal hepatocyte cell line QSG-7701.TC0 was taken as the highest concentration, diluted to 3 different concentrations of aloe-emodin, and used to treat human hepatoma HepG2 cells in vitro for the aloe-emodin group; another untreated HepG2 cells were set up as a blank group; cell proliferation inhibition rate was measured by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay after 48 hours. Cell migration assay and scratch assay were used to detect the cell migration in both groups. The effect of aloe-emodin on the expression of HepG2 cell F-actin was analyzed by high-content cell imaging system. The expressions of phosphorylated endothelial nitric oxide synthase (p-eNOS) and nuclear transcription factor-κB p65 (NF-κB p65) in HepG2 cells were detected by Western blot. Result: TC0 of aloe-emodin on human normal hepatocyte cell line QSG-7701 was 50 μmol·L-1. TC0 was taken as the highest concentration to treat HepG2 cells with aloe-emodin (10, 30, 50 μmol·L-1). Compared with blank group, The proliferation inhibition rate of aloe-emodin group increased after 48 hours of culture, indicating the dose escalation thpe (P<0.01), after 48 hours of culture, the scratch distance of aloe-emodin group was broadened, and the migrating ability of aloe-emodin group was decreased, indicating the dose decline type (P<0.01); F-actin area was shorten, disordered, irregular and fuzzy, indicating the dose decline type (P<0.01); the expression of NF-κB p65 in aloe-emodin group was increased, while the expression of p-eNOS was decreased, indicating the dose decline type (P<0.01). Conclusion: Aloe-emodin may reduce the proliferation and migration of HepG2 cells by increasing NF-κB p65, inhibiting the expression of p-eNOS, promoting the depolymerization of F-actin, and reducing the formation of microfilaments, with an effect in inhibiting tumor.关键词：aloe-emodin;hepatoma;migration;fibrous actin (F-actin)6|6|3更新时间：2024-01-04
- 摘要：Objective: To study the effect of Alismatis Rhizoma polysaccharide (AP) on glucose-lipid metabolism as well as the mRNA and protein expression of Adipo R2 and PPARα in diabetic rats, and discuss the potential mechanism. Method: Diabetic rats model was induced by intraperitoneal injection of streptozotocin (STZ), and the diabetic rats were randomly divided into six groups, including normal group, model group, Pioglitazone hydrochloride group,AP high, medium and low (400,200,100 mg ·kg-1) dose groups, n=8 in each group. The drug administration was given for 30 days. The body weight, liver weight, oral glucose tolerance test (OGTT), fasting blood glucose (FBG), fasting insulin (FINS), glycosylated serum protein (GSP), glycosylated hemoglobin (HbA1c), serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL) and liver histology index were compared. Immunohistochemical method was used to observe the level of insulin-like growth factors (IGF). The protein expression levels of Adipo R2 and PPARα in liver tissues of different groups were measured with Western blot, and the mRNA expression levels were measured with Real-time PCR. Result: As compared with the model group, AP could significantly reduce the body weight and liver weight of diabetic rats(P<0.01), as well as the levels of FBG, HbA1c and GSP, improve the indexes of TC, TG, LDL and HDL(P<0.05,P<0.01), and regulate the mRNA and protein expression levels of Adipo R2 and PPARα in liver tissues(P<0.05,P<0.01). Conclusion: AP can significantly adjust the glucolipid metabolism in diabetic rats, and its potential mechanism is related to the regulation of the expression levels of Adipo R2 and PPARα, with certain prevention and treatment effectfor diabetes complications and fatty accumulation in liver tissues.关键词：Alismatis Rhizoma polysaccharide;diabetes mellitus;glucose-lipid metabolism7|6|15更新时间：2024-01-04
- 摘要：Objective: The purpose of the study was to explore the effect of Bufei Yishen formula (BFYS) on cellular proliferation of pulmonary artery smooth muscle cells induced by transforming growth factor-β1 (TGF-β1)/bone morphogenetic protein-4 (BMP-4), furthermore, to explore the effect of BFYS on TGF-β1/Smad signaling pathway in the cells. Method: TGF-β1/BMP-4was used to induce proliferation of human pulmonary artery smooth muscle cells. The cells were divided normal group (10% normal rat serum), induced-proliferation group(10% normal rat serum with 7.5 μg·L-1TGF-β1 and 5 μg·L-1BMP-4), 4% BFYS serum group(6% normal rat serum with 7.5 μg·L-1TGF-β1 and 5 μg·L-1BMP-4 and 4% BFYS rat serum), 6% BFYS serum group (4% normal rat serum with 7.5 μg·L-1TGF-β1 and 5 μg·L-1BMP-4 and 6% BFYS rat serum) and 8% BFYS serum group (2% normal rat serum with 7.5 μg·L-1TGF-β1 and 5 μg·L-1BMP-4 and 8% BFYS rat serum). After 24 h induction, cell density was observed under a microscope; 5-bromo-2-deoxyuridine(BrdU) was used to detect the cellular proliferation;Western blot was used to detect the Smad 1,2,3,5 and p-Smad2/3 protein expression, and Real-time PCR was used to detect plasminogen activator inhibitor-1 (PAI-1), connective tissue growth factor(CTGF), dominant negative helix-loop-transcription factors-1 (ID-1)and ID-2 mRNA expression. Result: After 24 h of TGF-β1/BMP-4 treatment, the cells density was increased under micro-examination with significant increase in cellular proliferation ratio (P<0.05). BFYS inhibited TGF-β1/BMP-4-induced cellular proliferation in a dose dependent manner, with significant decrease in 8% concentration group(P<0.01). Both TGF-β1/BMP-4 and BFYS showed no significant effect on Smad1,2,3 and 5 proteins expression. However, p-Smad2/3 expression was significant increased by TGF-β1/BMP-4 induction (P<0.05) and 8% BFYS significantly reduced its expression (P<0.05). For assays of down-scream genes expression of TGF-β1/Smad pathway, TGF-β1/BMP-4 and BFYS showed no significant effect on PAI-1, ID-1and ID-2 mRNA expression. But TGF-β1/BMP-4 increased CTGF mRNA expression (P<0.01) and 8% BFYS inhibited TGF-β1/BMP-4 induced CTGF mRNA expression (P<0.05). Conclusion: BFYS reduced TGF-β1/BMP-4 induced cellular proliferation of human pulmonary arterial smooth muscle cells, and its mechanisms might be associated with inhibiting p-Smad2/3/CTGF pathway.关键词：transforming growth factor-β1 (TGF-β1)/bone morphogenetic protein-4 (BMP-4);Bufei Yishen formula;human pulmonary arterial smooth muscle cells(PASMCs);TGF-β1/Smad signaling;proliferation6|5|4更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of Jianpi Huashi granule on tyrosine hydroxylase, Monoamine oxidase and serotonin transporter expression in brain of rats with diarrhea-predominant irritable bowel syndrome (D-IBS), and explore its mechanism. Method: SD rats were randomly divided into normal group, model group, Jianpi Huashi granule low, middle and high dose groups (6.25, 12.5, 25 g·kg-1), and positive drug group (pinaverium bromide, 0.03 g·kg-1), n=10 in each group. Except the rats in normal group, all the other rats received intragastric administration of Sennae combined with restraint stress to establish D-IBS models. The changes of sucrose preference in different groups were observed at different time points, and Jianpi Huashi granule was used for 14 days. Then immunohistochemical method and Western blot were used to detect the expression levels of tyrosine hydroxylase, monoamine oxidase and serotonin transporter in brain. Result: In the D-IBS model group, the sucrose preference was decreased significantly, the expression of tyrosine hydroxylase in brain was significantly increased, and the expression levels of monoamine oxidase and serotonin transporter in brain were decreased significantly as compared with the normal control group (P<0.01). After treatment, the sucrose preference was significantly increased in medium-and high-dose Jianpi Huashi granule groups and positive drug group (P<0.05, P<0.01) ; except the relative expression of monoamine oxidase in positive drug group, the expression levels of tyrosine hydroxylase in all other treatment groups were decreased significantly and the expression levels of monoamine oxidase and serotonin transporter were increased significantly as compared with the model group (P<0.05, P<0.01). As compared with the positive drug group, the sucrose preference was increased significantly (P<0.05, P<0.01); the expression of tyrosine hydroxylase was decreased significantly (P<0.05, P<0.01); the expression of monoamine oxidase in middle and high-dose Jianpi Huashi granule groups and the positive expression of serotonin transporter in high-dose Jianpi Huashi granule group were increased significantly (P<0.05, P<0.01); the expression levels of monoamine oxidase and serotonin transporter were increased significantly in low, middle and high dose Jianpi Huashi granule groups (P<0.05, P<0.01). Conclusion: Jianpi Huashi granule reduced the content of serotonin transporter in brain of rats with D-IBS, which was closely related to down-regulating the expression of tyrosine hydroxylase and up-regulating monoamine oxidase and serotonin transporter in brain. This may be one of the therapeutic mechanisms of Jianpi Huashi granule for D-IBS.关键词：diarrhea-predominant irritable bowel syndrome(D-IBS);Jianpi Huashi granule;tyrosine hydroxylase;monoamine oxidase;5-hydroxyryptamine serotonin transporter6|6|2更新时间：2024-01-04
- 摘要：Objective: To observe the sedative, hypnotic and anxiolytic effects of Schisandrae Chinensis Fructus vinegar (SV) and explore its underlying mechanisms. Method: Fifty ICR male mice were randomly divided into blank control group (gavage distilled water, at a dose of 10 mL·kg-1), diazepam group (sedative experimental dose of 2 mg·kg-1, hypnosis and anti-anxiety experiment dose of 4 mg·kg-1), low-dose SV group, medium-dose SV group and high-dose SV group (corresponding doses by intragastric administration), for consecutive 7 days. The effect of SV on the locomotor activity of mice was observed with locomotor activity recorder. By using the synergistic sleeping experiment with SV and threshold-dose pentobarbital sodium, the effect of SV on the number of sleeping mice, the sleeping latency and sleeping time were observed. The anxiolytic effect of SV was observed by using elevated plus maze (EPM). The effects of SV on the contents of γ-aminobutyric acid (GABA) and glutamate (Glu) were observed by enzyme-linked immunosorbent assay (ELISA), and Western blot was used to observe the effect of SV on the expression levels of GABAAα1 and GABAAγ2 in the brain tissues. Result: As compared with those in the blank control group, the number of locomotor activities (P<0.05, P<0.01); the number of sleeping mice was increased significantly (P<0.05); the sleeping latency was significantly shortened and the sleeping time was significantly prolonged in the medium-dose and high-dose SV groups (P<0.05). The open-arms time percentage (OT) and open-arms entries percentage (OE) in EPM model mice were increased significantly in the medium-dose and high-dose SV groups (P<0.05); the content of GABA was increased significantly (P <0.05)and the content of Glu was decreased significantly in the brain tissues in the high-dose SV group only (P <0.05); the protein expression levels of GABAAα1 and GABAAγ2 were increased significantly in the medium-dose and high-dose SV groups (P<0.05, P<0.01). Conclusion: SV has sedative, hypnotic and anxiolytic effects, and its mechanism may be related to the regulation of GABA and Glu contents as well as the protein expression of GABAAα1 and GABAAγ2 in brain tissues.关键词：Schisandrae Chinensis Fructus vinegar (SV);sedative;hypnotic;anxiolytic7|7|2更新时间：2024-01-04
- 摘要：Objective: This experiment was used to investigate the protective effect of Tangzhiqing formula(TZQ), clearing prescription on learning, memory ability and apoptosis of hippocampal neurons in type 2 diabetic rats. Method: SD male rats were fed with high glucose and high fat diet and streptozotocin (STZ) (30 mg·kg-1) intraperitoneal injection to produce type 2 diabetes mellitus model. The model rats were randomly divided into model group, high, middle and low dose group,the daily dose was 12,6,3 g·kg-1,compared with normal control group. After 8 weeks, the Morris maze test was conducted. The pathological structure of hippocampus was observed by hematoxylin-eosin(HE) staining, and the apoptosis of hippocampal neurons was detected by TdT\|mediated dUTP nick end labeling(TUNEL) staining. Result: Compared with the model group,TZQ could significantly reduce the blood glucose of type 2 diabetic rats (P<0.05,P<0.01); reduce latency of escape (P<0.05,P<0.01); increase the number of crossing platform, the platform of swimming distance and swimming time(P<0.05,P<0.01); improve pathological of hippocampus tissue; significantly reduce the apoptotic index of hippocampal neurons (P<0.01). Conclusion: TZQ could significantly reduce the high glucose of type 2 diabetes model rats fed with high fat diet and injected low dose streptozotocin. TZQ improved insulin resistance, learning and memory ability in rats. TZQ also could protect hippocampal neurons and reduce apoptosis.关键词：Tangzhiqing formula;diabetic;learning and memory;hippocampal neurons;apoptosis7|6|3更新时间：2024-01-04
- 摘要：Objective: To observe the effect of Wenshen prescription on toll-like receptor 4 (TLR4)/interleukin-6 (IL-6) signaling pathway in chronic hepatitis B(HBV) patients with high ALT level and kidney Yang deficiency syndrome. Method: A total of 68 CHB patients with high ALT level treated in our hospital from July 2013 to May 2014 were selected as research objectives, with 34 cases in each group. Eligible patients were given Wenshen prescription combined with telbivudine (treatment group) or placebo combined with telbivudine (control group) for 24 weeks. Peripheral blood hepatitis B virus DNA, HBV markers, IL-6 were measured at week 0, 12 and 24.The mRNA expression of TLR4 in peripheral blood mononuclear cell (PBMC) was measured by Real-time PCR, and helper T cells (Th17)/regulatory T cells (Treg) ratio was measured by flow cytometry. Result: There was no significant difference at baseline between the two groups. With the increase of treatment time, HBV DNA negative conversion rate, HBeAg negative conversion rate and seroconversion rate of the two groups were gradually decreased. At week 24, treatment group was superior to control group in the rates of HBV DNA negative conversion, HBeAg negative conversion and seroconversion. During the treatment, the peripheral blood IL-6, TLR4 mRNA levels and Th17/Treg ratio first increased and later decreased in the treatment group. They reached the peak at week 12, which were significantly higher than the baseline (P<0.05). At week 12 and 24, the peripheral blood IL-6, TLR4 mRNA and Th17/Treg in treatment group were superior to those in control group (P<0.05). Conclusion: Wenshen prescription has an increased antiviral effect on telbivudine-treated CHB patients with high ALT level and kidney Yang deficiency syndrome, and its mechanism may be related to the regulation of Th17/Treg balance by TLR4/IL-6/STAT3 signaling pathway.关键词：hepatitis B;high alanine aminotransferase (ALT);latent pathogenic cold;warming kidney;Toll-like receptor 4;interleukin-6;Th17/Treg balance8|6|7更新时间：2024-01-04
- 摘要：Objective: To observe the effect of Tongqiao Huoxuefang combined with flunarizine on the prognosis of Qixu Xueyu type ischemic stroke patients. Method: A total of 100 ischemic stroke patients treated in our hospital January 2015 to June 2016 were randomly divided into control group (30 cases), and treatment A and B groups (35 cases in each group), and 50 health cases were selected into the control group. Patients in control group were treated with antiplatelet and anticoagulant treatment; in addition to the therapy of control group, treatment A and B groups were also treated with Tongqiao Huoxuefang alone as well as Tongqiao Huoxuefang combined with flunarizine. The symptom score, National Institute of Health Stroke Scale (NIHSS) score, infarct volume, clinical efficacy, max platelet aggregation rate (MAR), living ability (IADL score) and quality of life (SS-QOL), serum calcium and calmodulin kinase Ⅱ (CaMKⅡ) levels were compared and analyzed. Kaplan-Meier survival curves were drawn to compare the differences in the prognosis (improved Rankin scale), living ability (IADL score) and quality of life (SS-QOL) between the two groups, and explore CaMKⅡ-based efficacy and long-term prognosis after treatment. Result: After treatment, the scores of NIHSS and infarct volume were significantly lower than those before treatment, and the treatment group B was more obvious (P<0.05) than that in group A after 4 weeks of treatment (P<0.05). After 2 months of treatment, the living ability and quality of life in treatment A and B groups were significantly higher than those of the control group. Among them, treatment group B after 4 months of treatment was more obvious than group A (P<0.05). There was no significant difference in the total effective rate and the good prognosis rate in the treatment groups, but they were higher than those in control group. CaMKⅡ and MAR in control group and the treatment groups were significantly lower than those before treatment, and Ca2+ level was significantly higher, particularly in treatment group B (P<0.05). Conclusion: Tongqiao Huoxuefang combined with flunarizine have a better prognosis in the treatment of ischemic stroke, and can significantly alleviate the patient's neurological impairment and improve the living ability and quality of life. CaMKⅡ shows a higher predictive efficacy and long-term prognosis.关键词：ischemic stroke;Tongqiao Huoxuefang;aspirin;flunarizine;calmodulin kinase Ⅱ9|7|3更新时间：2024-01-04
- 摘要：Objective: To discuss the effect of Yupingfeng San combined with governor meridian long-snake moxibustion on serum interleukin-6 (IL-6), cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP) in patients with allergic rhinitis (AR). Method: A total of 100 patients with AR treated from June 2013 to June 2016 in Henan Province Hospital of Traditional Chinese Medicine were selected. According to the treatment method, all of the patients were divided into long-snake moxibustion group and Yupingfeng group, with 50 cases in each group.Long-snake moxibustion group was given cetirizine + governor vessel long-snake moxibustion treatment,Yupingfeng group on the basis was given Yupingfeng granules treatment, Serum IL-6, cGMP, cAMP levels were detected by enzyme-linked immunosorbent method. Before and after treatment, serum IL-6, cGMP, cAMP levels, sneezing, runny nose, stuffy nose, nasal itching disappearance time, efficacy, adverse reaction, recurrence of all of the patients were analyzed. Result: After treatment, serum IL-6, cGMP levels of Yupingfeng San group and long-snake moxibustion group were significantly lower than those before treatment; after treatment, serum IL-6, cGMP levels of Yupingfeng group were significantly lower than those of long-snake moxibustion group; after treatment, serum cAMP level of Yupingfeng group was significantly higher than that of long-snake moxibustion group, with statistically significant differences (P<0.05). Sneezing, runny nose, stuffy nose, nasal itching disappearance time of Yupingfeng group were significantly lower than those of long-snake moxibustion group; therapeutic response rate of Yupingfeng group was significantly higher than that of long-snake moxibustion group, with statistically significant differences (P<0.05) but no serious adverse reaction in the two groups. The recurrence rate of Yupingfeng San group was significantly lower than that of long-snake moxibustion group, with statistically significant differences (P<0.05). Conclusion: Yupingfeng San combined with governor meridian long-snake moxibustion can effectively improve the short-term and long-term efficacy of patients with AR, which may be related to more effective regulation of body immune function. It has a good safety, and is worth further clinical promotion.关键词：Yupingfeng San;governor meridian long-snake moxibustion;allergic rhinitis;interleukn-6;cyclic adenosine monophosphate;cyclic guanosine monophosphate6|6|3更新时间：2024-01-04
- 摘要：Objective: To study the clinical efficacy of Bushen Yifei Huaxian decoction on lung function, inflammatory mediator and quality of life in patients with chronic obstructive pulmonary disease (COPD) complicated with pulmonary fibrosis (PF). Method: The study selected 146 cases of patients with COPD-PF treated in Hebei Province Hospital of Traditional Chinese Medicine from May 2014 to July 2016.They were divided into two groups according to the random number table, with 73 cases in each group. Patients in the control group were treated with Salmeterol assigned powder inhaler, while the clinical group was also given Bushen Yifei Huaxian decoction in addition to the therapy of control group. Then the efficacy, lung function, inflammatory mediator and quality of life were compared after 3 months of treatment. Result: Compared with 82.19% in control group, the total effective rate of the clinical group was 93.15%. There was a significant increase (χ2=16.412, P<0.05). Compared with before treatment, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) of two groups decreased after treatment (P<0.05). Compared with control group, the values of TNF-α and IL-6 in patients of the clinical group were lower after treatment (P<0.05). Compared with before treatment, forced expiratory volume in one second (FEV1) and forced vital capacity (FVC) of the two groups after treatment increased (P<0.05). Compared with control group, the values of FEV1 and FVC in patients of the clinical group were higher after treatment (P<0.05). After treatment, scores of symptom, activity ability, disease influence and total scores in treatment group were lower than those in control group. There was a significant increase (P<0.05). Conclusion: Bushen Yifei Huaxian decoction is very effective in treating COPD-PF. It can significantly lower inflammatory mediators, and improve lung function and quality of life.关键词：Bushen Yifei Huaxian decoction;chronic obstructive pulmonary disease;pulmonary fibrosis;lung function;quality of life9|6|5更新时间：2024-01-04
- 摘要：Objective: To discuss the efficacy of modified Zhenwutang in treating chronic cardiac failure (CHF) with deficiency of Yang Qi, and its effect on levels of transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). Method: One hundred and twenty CHF patients were randomly divided into control group 60 cases and combination group 60 cases by random number table. Patients in control group took enalapril tablets, 10 mg/time, 1 time/day. Metoprolol tablets, 12.5 mg/time, 2 times/days. Spironolactone tablets, 20 mg/time, 1 time/day, and Digoxin tablets, 0.125-0.25 mg/days when necessary. In addition to the therapy of control group, patients in observation group were also given modified Zhenwutang, 1 dose/day. The course of treatment was 3 months. And a three-month follow-up period was set. Before and after treatment, cardiac function based on New York heart disease association (NYHA) and 6-minute walk test were evaluated, and left ventricular ejection fraction (LVEF), stroke volume (SV), cardiac output (CO) and E/A were recorded by echocardiogram. Lee congestive heart failure, traditional Chinese medicine (TCM) symptoms were scored. Levels of NT-proBNP, TGF-β1, CTGF, TNF-α and IL-1β were detected. And addition of dose or new medicine or hospitalization caused by exacerbation of NYHA fractionation and heart failure were evaluated after treatment and during the follow-up period. Result: By Ridit, the total effective rates of cardiac function and TCM symptoms in observation group were better than those in control group (P<0.05). And LVEF, CO, SV and E/A were higher than those in control group (P<0.05). After treatment and during the follow-up period, scores of Lee congestive heart failure and symptom of deficiency of Yang Qi were all lower than those in control group (P<0.01). The distance of 6-minute walk was longer than that in control group (P<0.01). And during treatment and follow-up period, the cumulative incidence of disease progress in control group was 42.86%, which was higher than 19.3% in observation group (χ2=7.333, P<0.01). And levels of NT-proBNP, TGF-β1, CTGF, TNF-α and IL-1β were lower than those in control group (P<0.01). Conclusion: Modified Zhenwutang on CHF with deficiency of Yang Qi can relieve symptoms, degree of heart failure and MF, increase the activity ability, and inhibit or delay myocardial remodeling, so as to stabilize the disease.关键词：chronic cardiac failure;Zhenwutang;myocardia fibrosis;transforming growth factor-β1;connective tissue growth factor;interleukin-1β;tumor necrosis factor-α7|6|8更新时间：2024-01-04
- 摘要：Objective: To observe the clinical efficacy of modified Yinpu Sini Simiao Shixiao palvis on long-term sequelae of pelvic inflammatory disease (SPID) with damp heat stasis syndrome, and its regulatory effect on immune inflammatory factors. Method: One hundred and thirty-two patients with SPID were randomly divided into control group and observation group by random number table. Both groups’ patients got Pengyanqing enema at night since the third day after the end of menstruation, for successively 10 days. Patients in control group got Fuke Qianjin capsule, 2 grains/time, 3 times/days. Patients in observation group got modified Yinpu Sini Simiao Shixiao palvis, 1 dose/day. The treatment lasted for 3 menstrual cycles, and discontinued during the menstrual period. Before and after treatment, the damp-heat stasis syndrome and pelvic signs were scored, and the quality of life was evaluated. Levels of T lymphocyte subsets (CD3+, CD4+, CD8+and CD4+/CD8+), interleukin-2 (IL-2), IL-4, IL-6, IL-10 and tumor necrosis factor-α (TNF-α) were detected. Result: The scores of damp-heat stasis syndrome and pelvic signs in observation group were lower than those in control group (P<0.01). And the score at all dimensions and the total score of life scale in observation group were higher than those in control group (P<0.01). Levels of CD4+ and CD4+/CD8+, IL-2, IL-4 and IL-10 in observation group were higher than those in control group (P<0.05,P<0.01), and levels of IL-6, IL-8 and TNF-α in observation group were lower than those in control group (P<0.01). The total effective rate in observation group was 95.16%, which was higher than 83.33% in control group (χ2=4.481, P<0.05). Conclusion: In addition to retention enema with Chinese medicine, modified Yinpu Sini Simiao Shixiao palvis is also given to relieve symptoms and signs, improve the quality of life and immunity of the patients, and regulate immune inflammatory factors.关键词：sequelae of pelvic inflammatory disease;damp-heat stasis syndrome;Yinpu Sini Simiao Shixiao palvis;T lymphocyte subsets;helper T cell6|6|3更新时间：2024-01-04
- 摘要：Objective: To explore the composition principles of ‘Xinrun(heat-moisturizing)’ treatment containing Angelicae Sinensis Radix or Chuanxiong Rhizoma based on the ‘Xinrun’ theory value of Danggui Shaoyaosan. Method: Formulas containing Angelicae Sinensis Radix or Chuanxiong Rhizoma related to ‘Xinrun’ treatment in the Chinese Prescription Database were collected. With the R language and R-studio software, the methods of data-mining including association rules and Apriori algorithm were used to extract and analyze the frequency of single medicine, the frequency of drug combination, and the association rules in core drug combinations. Result: A total of 91 ‘Xinrun’ formulas about Chuanxiong Rhizoma were included, and 22 core drug compositions and 19 association rules were obtained, 15 association rules of which had a confidence of 1, while the lift of 4 rules (Paeoniae Radix Alba=>Angelicae Sinensis Radix, Chuanxiong Rhizoma and Paeoniae Radix Alba=>Angelicae Sinensis Radix, Poria=>Angelicae Sinensis Radix, and Chuanxiong Rhizoma and Poria=>Angelicae Sinensis Radix) was greater than 1.A total of 284 ‘Xinrun’ formulas about Angelicae Sinensis Radix were found, and 25 core drug compositions and 19 rules were obtained, all of which had a confidence of 1.Herbs used in high frequency included blood conditioning medicine, Qi supplementing medicine, heat clearing medicine, wind expelling medicine, and Qi regulating medicine. Core drug combinations contained ‘Chuanxiong Rhizoma-Angelicae Sinensis Radix’ (74%), ‘Chuanxiong Rhizoma-Glycyrrhizae Radix et Rhizoma’ (55%), ‘Chuanxiong Rhizoma-Paeoniae Radix Alba’ (48%), ‘Angelicae Sinensis Radix-Glycyrrhizae Radix et Rhizoma’ (46%), ‘Angelicae Sinensis Radix-Paeoniae Radix Alba’ (42%), and ‘Angelicae Sinensis Radix-Rehmanniae Radix’ (36%). Angelicae Sinensis Radix, Chuanxiong Rhizoma, Paeoniae Radix Alba, Glycyrrhizae Radix et Rhizomacompatibilities were common in core drug combinations with high support degree. Conclusion: Based on the ‘Xinrun’ formulas, Angelicae Sinensis Radix or Chuanxiong Rhizoma were most frequently used with medicines which tonify the liver and kidney or soothe the liver and blood, often compatible with the medicines which tonify Qi and invigorate the spleen or expel the wind, indicating that the composition principles of ‘Xinrun’ treatment was based on the method of helping the body fluid flow indirectly. The core composition principles were basically the same with the compatibility characteristics of Danggui Shaoyaosan.关键词：Danggui Shaoyaosan;Angelicae Sinensis Radix;Chuanxiong Rhizoma;Xinrun;composition principles9|6|1更新时间：2024-01-04
- 摘要：Objective: To investigate the therapeutic mechanism of Yinchen Wulingsan based on network pharmacology. Method: Based on the Traditional Chinese Medicine Systems Pharmacology Database, the Protein Databank and the String database, the chemical compositions of Yinchen Wulingsan, corresponding targets and diseases were obtained respectively. Meanwhile, compound-target network, as well as target-disease network and protein-protein interaction network, were constructed. According to biological information annotation dotabase(DAVID) annotation databases, gene ontology biology process (GO-BP) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the targets were performed. Result: Totally 58 components, 103 targets, 11 hub targets and 262 diseases were obtained. The key targets involved tumor necrosis factor (TNF), peroxidase synthetase 2 (PTGS2), and estrogen receptor (ESR1), etc. The diseases mainly involved tumor, immune and endocrine. There were 146 GO-BP entries, including signal transduction entries, transcription and translation entries, proliferation promotion and apoptosis inhibition entries, cellular component entries, and oxidative stress entries. Besides, there were 108 KEGG pathways, involving tumor, virus, parasite and so on. Conclusion: The targets and mechanism corresponding to compounds of Yinchen Wulingsan were studied systematically in this study, providing ideas for further clinical researches and new drug development.关键词：Yinchen Wulingsan;network pharmacology;target;bioinformation;mechanism7|6|6更新时间：2024-01-04
- 摘要：Objective: To screen the main active components of Erzhiwan against liver fibrosis, and predict the targets and signal pathways for anti-hepatic fibrosis. Method: Based on the ADME computing method, Traditional Chinese Medicine System Pharmacology platform (TCMSP) and other databases, as well as reverse molecular docking server (DRAR-CPI), the active ingredients and the potential targets of of Erzhiwan for anti-hepatic fibrosis effects were predicted. Then by mapping with the anti-hepatic fibrosis targets in Human Genome annotation database (GeneCards), the anti-hepatic fibrosis targets of Erzhiwan were screened. Cytoscape 3.5.1 software was used to construct the active ingredient-target network of Erzhiwan was constructed; the biological information annotation database (DAVID) was used for enrichment analysis of gene ontology (GO) biological process and Kyoto Encyclopedia of genes andgenomes (KEGG) metabolic pathway. Result: The 18 active components were screened from Erzhiwan, which acted on 114 targets, mainly involving 8 signaling pathways and 13 biological processes. Conclusion: The bioactive components of Erzhiwan can play an anti-hepatic fibrosis role through transforming growth factor-β (TGF-β), mitogen activated protein kinase (MAPK), phosphatidyl inositol 3-kinase(PI3K)/serine/threonine protein kinase (Akt) signal pathway (PI3K/Akt), nuclear transcription factor-κB (NF-κB), peroxisome proliferators-activated receptor (PPAR) and other signal pathways, protein phosphorylation, signal transduction and other biological processes, embodying the characteristics of multiple components, multiple targets and pathways of traditional Chinese medicine. This study provides a theoretical basis for further research on the efficacy of Erzhiwan against liver fibrosis and the explanation on its mechanism of anti-hepatic fibrosis.关键词：Erzhiwan;liver fibrosis;network pharmacology;reverse molecular docking;target;pathway7|6|6更新时间：2024-01-04
- 摘要：Objective: Data mining method was adopted to analyze and summarize the medication regularity in the prescription drugs for lung cancer by the theory of ‘Fuzheng Quxie and Guben Qingyuan’, and discuss the mechanism of its action. Method: Effective prescriptions for treating lung cancer collected in outpatients of doctor Lin hongsheng in the department of oncology of Guang'anmen Hospital from August 2015 to August 2016 were screened out, and the ‘traditional Chinese medicine (TCM) inheritance auxiliary system (V2.50)’ was used for data mining and analysis of medication regularity. Result: A total of 524 prescriptions were involved in 182 literatures. The TCMs with frequent use of the prescriptions under the guidance of the theory of ‘Fuzheng Quxie and Guben Qingyuan’ were collected. The result preliminary proved that ‘Fuzheng Quxie and Guben Qingyuan’ was used in lung cancer throughout the TCM therapy. Conclusion: The theory of ‘Fuzheng Quxie and Guben Qingyuan’ gives guidance to the treatment of lung cancer in different stages, the treatment of modern medicine is dominated by ‘Fuzheng’; when the treatment and complications of modern medical treatment cannot be tolerated, the therapy of ‘Quxie Qingyuan’ TCM is mainly used; after the use of modern medical treatment, in the maintenance and consolidation period stage, both ‘Fuzheng Quxie and Guben Qingyuan’ were given equal stress. The medication regularity was summarized to further verify the mechanism of ‘Fuzheng Quxie and Guben Qingyuan’ throughout the whole process of the TCM treatment of lung cancer.关键词：lung cancer;LIN Hong-sheng;Fuzheng Quxie;Guben Qingyuan;medication regularity;action mechanism6|6|3更新时间：2024-01-04
- 摘要：Bryophyllum pinnatum, a Bryophllurm plant in Crassulaceae species, is a cheap traditional herb in many countries, which can be cultivated and collected throughout the year. It has high ornamental value and rich chemical constituents such as quercetin and other flavonoids,aconitic acid and other acids,18a-oleanane and other terpenoids,de-cenyl phenanthrene and other quinones,as well as inorganic elements. B. pinnatum has a wide range of pharmacological effects due to rich material basis. Some studies have shown that its anti-tumor,anti-oxidation,anti-inflammatory and analgesic effects are related to a large amount of flavonoids,and it also has many other effects such as antibacterial,antiviral,anesthetic,analgesic,anti-convulsion,liver protection and immune regulation effects. The wide range of pharmacological effects, cheap price and easy cultivation of B. pinnatum make it achieve high economic and social benefits, with great potential for development and broad market prospects, so the research on B. pinnatum is hot in recent years. However,there are only a few studies on the chemical compositions and pharmacological effects of B. pinnatum at present, and few overviews are reported. In order to reasonably develop B. pinnatum resources, a large amount of domestic and foreign papers on B. pinnatum were accessed to introduce and summarize the research status both at home and abroad, chemical compositions and pharmacological effects of B. pinnatum, hoping to provide scientific basis for the further research of B. pinnatum.关键词：Bryophyllum pinnatum;chemical composition;pharmacological effects;research progress6|6|0更新时间：2024-01-04
- 摘要：Pancreatic cancer is a malignant tumor of the digestive tract. However, its mechanism is not fully understood because of the multidrug resistance of tumor cells. In order to solve the drug resistance problem, scientists are committed to developing new chemotherapeutic methods. With the development of science and technology, more and more scientific and technological means are expected to provide brand-new treatment methods for treating pancreatic cancer. However, it still takes time from the successful development to clinical application, and the current problem of pancreatic cancer treatment still cannot be solved. Therefore, it is necessary to solve the drug resistance in the clinical treatment of pancreatic cancer at this stage. With the continuous improvement of China's international influence, the traditional Chinese culture has also drawn more and more attention. It is worth mentioning that in recent years, the government has attached more importance to the culture of traditional Chinese medicine and made increasing efforts in driving it. Chinese herbal medicine has been a valuable legacy for Chinese people to struggle with diseases, with a long history and low toxic and side effects, and more affordable by patients. In recent years, it has been reported in the literatures that Chinese herbal medicine compounds have a significant effect in inhibiting the proliferation of tumor cells and can even reverse the drug resistance of tumor cells. Clinical studies have also found that Chinese herbal medicine has a better therapeutic effect on tumor treatment. So far, the literatures on the effect of traditional Chinese medicines(TCM) in reversing the drug resistance of pancreatic cancer in recent years cannot reflect the advance of studies in the field. In this paper, the mechanism of drug resistance in the abnormal expression of pancreatic cancer drug resistance, and the effects of anti-tetrandrine, emodin, astaxanthin, curcumin and other TCM in reversing the progress of tumor drug resistance were reviewed.关键词：pancreatic cancer;drug resistance mechanism;traditional Chinese medicine;reverse drug resistance6|6|4更新时间：2024-01-04
- 摘要：Objective: To collect and analyze the reported literature of Melastoma dodecandrum,summarize the paper distribution,chemical compositions,pharmacological activity,quality control,clinical application and patent application status,and provide reference and literature for its clinical application and further development. Method: With “CNKI” and “Pubmed” as the search platform,“key words” and “abstract” as search terms,“diren”,“gagoulu”,“Melastoma dodecandrum Lour.” and “M. dodecandrum” as search words,the papers and patents of M. dodecandrum at home and abroad from 1973 to 2017 were retrieved in this study. Result: 119 papers and 139 patents were obtained,mainly about agriculture and forestry cultivation,chemical composition,quality control and pharmacological activity research in papers,and mainly about compound products in patents. At present,83 chemical constituents were isolated and identified from M. dodecandrum,mainly including flavonoids. Quality control indicators mainly included gallic acid,quercetin and so on, and the pharmacological research was mainly on hypoglycemic effect. Conclusion: M. dodecandrum had significant hemostatic effects with rich resource and high market competitiveness. However,the local standards were not complete,lacking specific indicators to evaluate the quality of medicinal herbs. Therefore, it is necessary to explore the mechanism of hemostasis in depth,study the basis of its active substances,and establish the relationship between spectrum efficiency and efficacy so as to control the quality of medicinal herbs,and promote the utilization and development of natural medicine resources.关键词：Melastoma dodecandrum;literature distribution;patent;research status;clearing heat and removing toxicity8|6|1更新时间：2024-01-04
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