最新刊期
卷 24 ， 期 12 ， 2018
- 摘要：Objective: To establish a near infrared spectral discriminant model of crude products,counterfeit products and processed products of Calamina by principal component analysis and cluster analysis. Method: Near infrared spectra of crude products,counterfeit products and processed products of Calamina were collected.Each category sample was randomly divided into training set and testing set.The spectral preprocessing methods and modeling spectral bands were screened,the principal component discriminant analysis model and the cluster analysis model were established respectively. Result: Spectrs were preprocessed by the first derivative.The characteristic spectral band of the principal component discriminant analysis model was 4 800-4 000 cm-1,and the characteristic spectral bands of the cluster analysis model were 7 300-7 000,4 800-4 000 cm-1.In the principal component discriminant analysis model,the prediction accuracy rate was 94.34%.In the cluster analysis model,the prediction accuracy rate was 96.23%. Conclusion: The principal component analysis model and cluster analysis model of near infrared spectra can be used for identification of crude products,counterfeit products and processed products of Calamina.关键词：Calamina;detoxification and eyesight;near infrared spectroscopy;principal component analysis;cluster analysis;processed products;counterfeit products4|6|8更新时间：2024-01-04
- 摘要：Objective: To improve pulverizing process of Canhuang tablets for promoting its quality stability. Method: Based on the preparation method of composite particles of Chinese medicine,3 kinds of ultrafine grinding process was used to prepare Canhuang tablets.The powder characteristics were investigated parallel with morphology and thermal stability evaluation by scanning electron microscopy and differential scanning calorimetry.Quality stability of Canhuang tablets with different ultrafine grinding processes were evaluated under high temperature,high humidity and strong light conditions. Result: Process 1:Coptidis Rhizoma,Indigo Naturalis,Alumen and Curcumae Radix were mixed in proportion and pulverized 20 min.Process 2:Coptidis Rhizoma and Curcumae Radix were pulverized 25 min together;Indigo Naturalis and Alumen pulverized 10 min together,then 2 kinds of powder mixed evenly.Process 3:Coptidis Rhizoma and Curcumae Radix were ultrafine grinded for 25 min together,added Indigo Naturalis continue to pulverize 10 min,then added Alumen keep on pulverizing for 5 min.The morphology and powder properties of ultrafine powders with 3 kinds of processes were obviously different,specific heat capacities of them were 262.1,242.7,295.9 J·g-1.The results of high temperature,high humidity and strong light test showed that only Canhuang tablets with process 3 was qualified with good stability. Conclusion: The ultrafine grinding process 3 under composite particles design concept can improve the powder characteristics and plasticity of tableting,and the quality stability of Canhuang tablets under this process is greatly improved.关键词：ultrafine grinding;powder;Canhuang tablets;quality;stability;influencing factors test;differential scanning calorimetry6|6|2更新时间：2024-01-04
- 摘要：Objective: To compare the contents of seven chemical constituents in Jiawei Ermiao granules and traditional decoction,and to establish their fingerprints and evaluate the consistency of material basis. Method: Based on the established analysis method of determination and fingerprint in previous experiments,the contents of phellodendrine,berberine hydrochloride,jatrorrhizine hydrochloride,(R,S)-goitrin,astilbin,hesperidin,atracylenolide Ⅲ in Jiawei Ermiao granules and traditional decoction were determined.Taking berberine hydrochloride as the reference peak,the similarity of the granules and traditional decoction was carried out by similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2012 edition).The difference of relative peak area of the granules and decoction was analyzed by principal component analysis (PCA),and the decoction was decocted with decomposed recipes,the influence of decomposed recipes on fingerprint of Jiawei Ermiao decoction from the qualitative evaluation. Result: The contents of astilbin,jatrorrhizine hydrochloride,atracylenolide Ⅲ in the granules and decoction had no significant difference,the contents of phellodendrine,berberine hydrochloride,hesperidin and (R,S)-goitrin in the granules was higher than those of the traditional decoction.Twenty common peaks were indicated in the granules and 19 common peaks were indicated in the traditional decoction.There were no significant changes in the overall material basis between the granules and decoction.PCA results showed that the relative peak area of the traditional decoction was significantly different from that of the granules.There was no new compound in HPLC fingerprints of decoction with decomposed recipes. Conclusion: The established fingerprint reflects the overall material basis of Jiawei Ermiao granules and traditional decoction,the material basis of the granules and decoction is basically the same.The contents of these seven components combined with the fingerprints can reflect the intrinsic quality of the granules and traditional decoction,and provide reference for their clinical application.关键词：Jiawei Ermiao prescription;granules;decoction;cervical cancer;fingerprint;berberine hydrochloride;(R,S)-goitrin4|6|4更新时间：2024-01-04
- 摘要：Objective: To establish a DNA barcoding based method for guarantee the species authenticity of Anemarrhenae Rhizoma seeds. Method: Thirty original plant materials and nine raw materials have been collected for obtaining the reference DNA barcode sequences.The clone sequencing technique was employed to confirm the reliability of reference sequences.Fifty-one samples of Anemarrhenae Rhizoma seeds pending to test have been collected,and DNA barcodes of these samples were obtained for taxonomic assignment by BLAST method,genetic distance method and neighbor-joining(NJ) phylogenetic tree method. Result: For the original plants and raw materials,their internal transcribed spacer 2(ITS2) sequences could not be obtained stably as a result of intra-genomic heterogeneity.Meanwhile,their psbA-trnH sequences could be successfully obtained.Their psbA-trnH sequences could be divide into six haplotypes,including three variation sites and two insertions/deletions.The maximum intra-specific distance was 0.003 5,whereas the minimum inter-specific distance was 0.1.Their psbA-trnH sequences were composed of one clade at the NJ dendrogram.There were 153 psbA-trnH sequences have been obtained and all can be assigned to be Anemarrhena asphodeloides with BLAST method,genetic distance method and NJ phylogenetic tree method. Conclusion: The DNA barcoding technology is a reliable method for species identification of Anemarrhenae Rhizoma seeds.All the samples of Anemarrhenae Rhizoma seeds pending to test are in accordance with the origin of that ruled on the 2015 edition of Chinese Pharmacopoeia and no adulterant has been detected.关键词：Anemarrhenae Rhizoma;seeds;DNA barcoding;psbA-trnH;identification;original plant7|6|6更新时间：2024-01-04
- 摘要：Objective: To investigate the methylation pattern of Salvia miltiorrhiza copalyl diphosphate synthase 1 (SmCPS1) promoter in different tissues of S. miltiorrhiza and its correlation with SmCPS1 tissue-differential expression pattern. Method: Bisulfite conversion method was used to detect the methylation rate of SmCPS1 promoter in -1 021 bp (translation start site +1). The expression of SmCPS1 was determined by Real-time quantitative polymerase chain reaction method(Real-time PCR). Result: Methylation sites were mainly distributed in the promoter region between -750 bp to-500 bp,rarely in region below -450 bp. Among the 300 examined sites,72 sites (about 24%) showed a tissue-differential manner. 18 transcription binding regions were found to have methylated sites,and 10 of these regions showed differential methylated sites (DMS).A total of 21 DMS were found to be significantly correlated with SmCPS1(P<0.01);7 DMS,which were in negative correlation with SmCPS1,were found to be mainly distributed in the promoter region between -632 bp to -450 bp;the other 14 DMS,which were in positive correlation with SmCPS1,were distributed in the region beyond -632 bp and below -450 bp. Conclusion: Promoter methylation difference may be the cause of tissue-differential expression pattern of SmCPS1 in S. miltiorrhiza.关键词：Salvia miltiorrhiza;Salvia miltiorrhiza copalyl diphosphate synthase 1 (SmCPS1);methylation;promoter6|5|0更新时间：2024-01-04
- 摘要：Objective: This study aimed to analysis the diversity of endophytic fungal communities in Astragali Radix,and provide a reference for study on the directional processing technology of active ingredients in this herb. Method: The 18S rDNA NS1-Fung region of endophytic fungal communities in Astragali Radix was sequenced by Illumina MiSeq high-throughput sequencing technology,abundance of species and other bioinformation were analyzed. Result: The numbers of effective sequences and operational taxonomic units for samples were 29 677 and 188,respectively.The number of sequencing was close to saturation,and the sequencing data volume was reasonable.The main endophytic fungi of Astragali Radix belonged to 5 genera,such as Hemileia,Gibberella,Ascobolus,Pseudogymnoascus and Aspergillus. Conclusion: By and large,the diversity of endophytic fungi in Astragali Radix is low.Hemileia and Gibberella are the dominant endophytic fungi in Astragali Radix.关键词：Astragali Radix;endophytic fungi;high-throughput sequencing;diversity;directional processing technology;tonifying Qi and lifting Yang;operational taxonomic unit7|7|4更新时间：2024-01-04
- 摘要：Objective: To determine the contents of macroelements and trace elements in bone marrow powder (BMPD),bone marrow oil (BMO),bone marrow protein (BMP) and bone protein (BP) from 4 types of Xinjiang characteristic animals:sheep,bovine,horse,and camel, measure the molecular weight of BMP,and provide a reference for improving the quality standard of traditional animal drugs. Method: Inductively coupled plasma-atomic emission spectrometry(ICP-OES) was used to determine the contents of 15 elements including Ca, K,Na,B,P,Ba,Cr,Cu,Fe,Mg,Mn,Ni,Sr,Zn and Si;1-DE electrophoresis was used to identify the molecular weight of BMP. Result: Four types of animals BMP bands were all shown at 66 kDa; the trace elements showed good linearity; detection limits range was 0.13-3.99 μg·L -1,with precision RSD of 0.2%-2.7% and recovery rate of 92.5%-101.4%. A large number of constant and trace elements were enriched in BP and BMP,of which horse BMP showed the highest level of beneficial trace elements, with a content of 24.92,10.83 mg·g-1 respectively for Ca and P;the content of Na,K,Ca,P,Mg was as follows:BP>BMP>BMPD>BMO;the content of Fe,Cu,Zn,Mn and other elements:BMP>BP>BMPD>BMO. Conclusion: The established ICP-OES method was suitable and highly sensitive for the comparative analysis of trace elements in BM and bone samples from different sources of domestic animals. It was suggested that,protein and peptide should be extracted intensively to improve not only the comprehensive utilization of bone and bone marrow, and also could provide theoretical basis for the research and development of functional foods and medicines.关键词：duolang sheep;Xinjiang brown cattle;Yili horse;Xinjiang bactrian camel;bone marrow protein;bone protein;macroelements;trace elements4|6|3更新时间：2024-01-04
- 摘要：Objective: To develop a method for determining 10 components in Fufang Daqingye mixture. Method: The quantitative analysis was carried out on a column of Waters CORTECS-C18(4.6 mm×150 mm, 2.7 μm) by UPLC-PDA, and eluted with a mobile phase of methanol (A)-0.4% formic acid solution (B) in a gradient mode (0-10 min, 10%-3%A; 10-25 min, 3%-35%A; 25-35 min, 35%-36%A; 35-55 min, 36%-80%A) under a flow rate of 0.3 mL·min-1 at 30℃. The detection wavelength was set at 290 nm in first 10 min, then changed to 330 nm between 10 and 38 min, and later changed to 250 nm between 38 and 55 min. Result: The linear ranges of gallic acid, neochlorogenic acid, chlorogenic acid, cyclohexanecarboxylic acid, isochlorogenic acid A, isochlorogenic acid B, nodakenin, isochlorogenic acid C, chrysophanol and emodin were 0.47-47.02, 1.00-100.14, 1.40-140.18, 1.15-115.11, 0.41-41.17, 0.32-32.11, 0.70-70.48, 0.67-67.22, 0.11-10.53, 0.12-12.29 ng. The recoveries were 99.4%, 99.7%, 99.8%, 99.7%, 99.4%, 99.3%, 99.8%, 99.4%, 99.4% and 99.3%, respectively. The relative standard deviations were 1.0%, 0.6%, 0.6%, 0.6%, 1.0%, 0.9%, 0.8%, 0.9%, 1.2% and 1.0%, respectively (n=6). Conclusion: This method is simple, accurate, reproducible and convenient for the quality control over Fufang Daqingye mixture.关键词：UPLC;Fufang Daqingye mixture;simultaneous determination;wavelengths switching4|6|2更新时间：2024-01-04
- 摘要：Objective: Melodinus (Apocynaceae) plants have been used in folks in China for a long time. Alkaloids from Melodinus genus have showed significant antitumor, antibacterial and antifertility activities. This study focuses on the chemical constituents of alkaloids from M. khasianus. Method: The compounds were isolated and purified by silica gel column chromatography, reversed-phase C18column chromatography and preparative liquid chromatography. The compounds were screened out for prostate cancer cells (PC-3), lung cancer cells (A549), gastric cancer cells (HGC-27) and human leukemia cells (HL-60) by MTT method. Result: Their chemical structures were established by their physicochemical properties and spectral data. Twelve compounds were isolated and characterized from the chloroform fraction of ethanol extract in M. khasianus. Their structures were identified as (+)epi-16α-hydro-14, 15vincamine (1), 16β,21β-epoxy-vincadine (2), melodinines G (3), melodinines P (4), melodinines N (5), 16β-hydroxy-19S-vindolinine (6), 16β-hydroxy-19R-vindolinine (7), 15α-hydroxy-14,15-dihydrovindolinine (8), melodinines T (9), 19-epimeloscandonine (10), meloscandonine (11) and scandine (12). Conclusion: Compounds 1-10 were isolated from M. khasianus for the first time. The cytotoxicity of these compounds was evaluated by MTT method in vitro. Compounds 4 and 5 displayed an inhibitory effect on the proliferation of prostate cancer cells (PC-3), lung cancer cells (A549), gastric cancer cells (HGC-27) and human leukemia cells (HL-60).关键词：Melodinus;Melodinus khasianus;alkaloids;cytotoxicity4|6|3更新时间：2024-01-04
- 摘要：Objective: To study the effect of Bistortae Rhizoma from different production places and colors on growth metabolism of Escherichia coli,and investigate its inhibitory effect on E. coli from the standpoint of biological thermodynamics. Method: The thermal spectrum curve reflecting the growth and metabolism of E. coli affected by 18 batches of Bistortae Rhizoma was determined by using microcalorimetry. Then the quantitative thermokinetic parameters,such as peak time(t1,t2),maximum heat-output(Pmax1,Pmax2),growth rate constant(k1,k2),and inhibition rate(I) obtained from these curves were analyzed. Result: Bistortae Rhizoma from Hebei,Shandong and Anhui could prolong the peak time of E. coli in the second growth exponential period (t2),and reduce the maximum thermal power (Pmax2) and growth rate (k2),different from the samples from Hubei. All samples from four production places had no influence on the parameters (t1,Pmax1,and k1) in first growth exponential period. Bistortae Rhizoma from different production places showed different I values on E. coli, between 20%-50% for the samples from Hebei, Shandong and Anhui, while the I value was lower for the samples from Hubei. The Bistortae Rhizoma samples from the same batch but with different colors also showed difference in inhibitory rate. A total of 7 batches of brownish red Bistortae Rhizoma showed higher inhibition rate (I) than its prunosus ones in all 9 batches. Conclusion: The Bistortae Rhizoma from different places and colors have different degrees of inhibitory effect on the growth and metabolism of E. coli, and the factors leading to the difference still need further study.关键词：Bistortae Rhizoma;microcalorimetry;antibacterial;Escherichia coli6|6|3更新时间：2024-01-04
- 摘要：Objective: To study the inhibitory effect of Zuojinfang and its main ingredient berberine on epithelial-mesenchymal transition of human gastric cancer cell line SGC7901. Method: Zuojinfang freeze-dried powder was prepared. High-performance liquid chromatography (HPLC) was adopted to define the purity and content of freeze-dried powder. Berberine standards were compared, and single drug content of freeze-dried powder was detected. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay was used to detect the effects of Zuojinfang and berberine on the growth of SGC7901 cells, and the concentration of the drug was determined. transwell chamber was used to detect the migration and invasion of cells. The expressions of E-cadherin, N-cadherin were detected by Western blot. Result: Prepared Zuojinfang freeze-dried powder contained 9.85% berberine. The median inhibitory concentration (IC50) of Zuojinfang was 165 mg·L-1. Zuojinfang (165 mg·L-1) and berberine (16.3 mg·L-1) were adopted for the subsequent experiment. Scratch and transwell experiments showed that Zuojinfang and berberine could significantly inhibit the migration and invasion of SGC7901 cells. Western blot analysis showed that Zuojinfang and berberine could significantly up-regulate the expression of E-cadherin in SGC7901 cells and down-regulate the expression of N-cadherin. The expressions of E-cadherin and N-cadherin were statistically significant different (P<0.05). Conclusion: Zuojinfang and berberine could inhibit the growth, migration and invasion of SGC7901 cells and inhibit the epithelial mesenchymal transition. Berberine is the main effective component in compound prescription.关键词：Zuojinfang;berberine;gastric cancer SGC7901;epithelial-mesenchymal transition (EMT);tumor metastasis6|6|4更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of Duanteng Yimu decoction (DTYMD) on the expression of NF-κB receptor activator ligand (RANKL) pathway and matrix metalloproteinase-9 (MMP-9) in osteoclasts and the mechanism of bone protection. Method: In vitro osteoclast culture system was established by inducing RAW264.7 macrophages. Blank group, model group, methotrexate group (2 mg·L-1) and DTYMD groups (400, 600, 800, 1 000 mg·L-1) were set up. The osteoblasts were identified by anti-tartaric acid phosphatase (TRAP) staining. The survival rate of each group was detected by cell counting kit-8 (CCK-8) method. The protein expression of RANKL signal pathway was detected by Western blot. The expression of MMP-9 was detected by enzyme-linked immunosorbent assay (ELISA). Result: Compared with model group, the number of fusion cells in DTYMD 400, 600 mg·L-1 concentration group decreased, but the difference was not statistically significant; the number of fusion cells was significantly decreased in DTYMD 800, 1 000 mg·L-1 concentration groups (P<0.01). CCK-8 results showed significant decreases in survival rates of DTYMD (600, 800, 1 000 mg·L-1) and methotrexate (2 mg·L-1) compared with model group (P<0.05, P<0.01). Western blot results showed that the protein expression of RANKL decreased in DTYMD 1 000 mg·L-1 and methotrexate 2 mg·L-1 groups (P<0.05) compared with model group. The protein expression of OPG in DTYMD group had no significant change. The results of ELISA showed that the expression of MMP-9 decreased in DTYMD concentration group and methotrexate group compared with the model group; and the higher the DTYMD concentration was, the more significantly decreased(P<0.05). Conclusion: DTYMD may protect bone cells by down-regulating the expressions of RANKL and MMP-9 and inhibiting the differentiation and proliferation of osteoclasts.关键词：Duanteng Yimu decoction;rheumatoid arthritis;osteoclast;NF-κB receptor activator ligand (RANKL);matrix metalloproteinase-9 (MMP-9)9|6|5更新时间：2024-01-04
- 摘要：Objective: To research the antioxidant activity of Chaihu Shugansan (CSGS) from different compatibility rules in vitro, further identify its antioxidant components and evaluate its regulation ability in the treatment of CSGS in depression. Method: After CSGS was divided into eight compatibility groups, their free radical scavenging activity was determined respectively by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) method, with the mass concentration value at a clearance rate of 50%(IC50) as an evaluation index. Orthogonal partial least squares (OPLS) method was used to investigate the correlation between the characteristic peaks and the efficacy of CSGS, and subsequently the significant active ingredients were identified by the variable importance of projection (VIP) based on the high performance liquid chromatography (HPLC) characteristic chromatograms. SYBYL 2.0 software was used for molecular docking studies of the antioxidant components in CSGS and regulation of CSGS treatment on depression-related enzymes. Finally, the binding capacity of small molecules and macromolecules was determined on the basis of total score value. Result: The results demonstrated that the contribution order of eight herbs for antioxidant activity was as follows:Aurantii Fructus>Citri Reticulatae Pericarpium>Chuanxiong Rhizoma>Bupleuri Radix>Cyperi Rhizoma>Paeoniae Radix Alba>Glycyrrhizae Radix et Rhizoma. Besides, three ingredients including hesperidin, neohesperidin and narirutin were identified as the antioxidant activity components. Docking results of both antioxidant components in CSGS and the enzymes regulated by CSGS in the treatment of depression showed a good binding ability. Among them, the binding energy of hesperidin and tryptophanase (TPH), neohesperidin and superoxide dismutase (SOD), narirutin and tryptophan 2,3-dioxygenase (TPHD) was 9.088 7, 8.734 0, 9.271 8, respectively. Conclusion: Hesperidin, neohesperidin and narirutin might be the main effective components of CSGS for the antioxidant activity in vitro and they play a regulatory role in the CSGS treatment of depression.关键词：Chaihu Shugansan;DPPH free radical scavenging ability;antioxidant activity in vitro;molecular docking8|8|5更新时间：2024-01-04
- 摘要：Objective: To explore the effect of α-hederin (α-HN) on the proliferation and apoptosis in melanoma B16 cells and its underling mechanism. Method: The melanoma B16 cells were conventionally cultivated to the logarithmic phase and treated with different concentrations of α-HN (50, 100, 200 μmol·L-1). B16 cells untreated with α-HN were set up as blank group. The cell proliferation was detected by 3-(4, 5-dimethyl thiazole)-2, 5-diphenyl 4 azole nitrogen bromine salt (MTT) method after 24, 48, 72 h of treatment, and the apoptosis rate, activities of Caspase-3 and Caspase-9, expressions of Bcl-2, Bax, phosphorylation phosphatidyl inositol-3-hydroxy kinase (p-PI3K), phosphorylation protein kinase B (p-Akt) and phosphorylation rapamycin target protein (p-mTOR) were evaluated by flow cytometry, Caspase activity detection kits and Western blot, respectively. Result: Compared with blank group, the cell proliferation inhibition rate in α-HN groups was increased significantly after being treated with 50, 100, 200 μmol·L-1 α-HN for 24, 48, 72 h (P<0.01), and B16 cell proliferation inhibition rate increased significantly; compared with blank group, the cell apoptosis rate was increased, the activities of Caspase-3 and Caspase-9 were enhanced, the Bcl-2 level reduced, and Bax level increased in α-HN groups, with statistically significant differences (P<0.01); compared with blank group, expressions of p-PI3K, p-Akt and p-mTOR were reduced in α-HN groups, with statistically significant differences (P<0.05, P<0.01). Conclusion: α-HN has the effects in inhibiting cell proliferation and inducing apoptosis in melanoma B16 cells, which may be related with the inhibition of PI3K/Akt/mTOR signaling pathway, with a good application prospect in the treatment of melanoma.关键词：melanoma;α-hederin (α-HN);proliferation;apoptosis;phosphatidyl inositol-3-hydroxy kinase (PI3K)/protein kinase B (Akt)/rapamycin target protein (mTOR) signaling pathway7|5|5更新时间：2024-01-04
- 摘要：Objective: To observe the effect of Shaoyaotang on the expression of high mobility group protein B1 (HMGB1), adapter protein myeloid differentiation factor 88(MyD88) and nuclear transcription factor-κB(NF-κB) in the colon tissues of rats with damp-heat type ulcerative colitis (UC), and explore the mechanism of Shaoyaotang damp-heat UC intervention. Method: Sixty male and sixty female Wistar rats were divided into blank group, model group, Shaoyaotang high, medium and low dose groups, and sulfasalazine group. The rat model of damp-heat type UC was established by 2,4,6-trinitrobenzene sulfonic acid (TNBS) combined with ethanol. High, medium and low doses of Shaoyaotang were given by intragastric administration, and the sulfasalazine was ground into powder and configured to be filled with liquid of equal volume. The rats in the blank group and model group were given with normal saline for continuous 21 days. Then colonic tissues were harvested.The content of gene was detected by Real-time PCR, and the content of protein was detected by Western blot. The pathological sections were observed by hematoxylin-eosin (HE) staining. Result: As compared with the blank group, the protein and mRNA expressions of HMGB1, MyD88 and NF-κB were significantly increased in the model group (P<0.05). As compared with the model group, the expressions of HMGB1, MyD88, NF-κB were decreased in Shaoyaotang groups and Sulfasalazine group (P<0.05). The effect was most significant in Shaoyaotang high dose group and sulfasalazine group (P<0.05). Conclusion: Shaoyaotang can regulate HMGB1, inhibit MyD88 and NF-κB in TLRs signaling pathway and reduce the inflammatory reaction of damp-heat type UC.关键词：Shaoyaotang;ulcerative colitis;damp-heat type;high mobility group protein B1(HMGB1);adapter protein myeloid differentiation factor 88(MyD88);nuclear transcription factor-κB(NF-κB)7|5|9更新时间：2024-01-04
- 摘要：Objective: To investigate the pharmacological effects of Aquilariae Lignum Resinatum ultrafine powder and fine powder about its anti-myocardial ischemia, anti-anoxia, analgesia and sedation effects, and compare with Syringa pinnatifolia var. alashanensis ultrafine powder. Method: Coronary artery ligation method was used to establish models of myocardial ischemia in rats. The myocardial infarction area, electrocardiogram, serum creatine kinase (CK), superoxide dismutase (SOD) and lactate dehydrogenase (LDH) were observed in each group. Survival time of mice in both Aquilariae Lignum Resinatum and S. pinnatifolia var. alashanensis groups was observed by Soda Lime method. Acetic acid writhing method and pentobarbital sodium synergetic methodwere used to observe the analgesic and sedative effects of Aquilariae Lignum Resinatum and S. pinnatifolia var. alashanensis in mice. Result: Aquilariae Lignum Resinatum ultrafine powder, fine powder and S.pinnatifolia var. alashanensis ultrafine powder had different degrees of effects on myocardial ischemia in rats:reduced the contents of serum CK and LDH, increased the content of SOD in serum, decreased the myocardial ischemia area and ameliorated the ECG performance(P<0.05,P<0.01).Aquilariae Lignum Resinatum ultrafine powder 0.75, 0.375 g·kg-1dose groups were significantly better than the fine powder(P<0.05); the same dose of Aquilariae Lignum Resinatumu ltrafine powder and S. pinnatifolia var. alashanensis ultrafine powder showed equivalent efficacy; the survival time of mice under hypoxia was prolonged in various groups (P<0.05), and the effect of S.pinnatifolia var. alashanensis ultrafine powder was better than that of Aquilariae Lignum Resinatum ultrafine powder at the same dose(P<0.05); the group of Aquilariae Lignum Resinatum ultrafine powder and S. pinnatifolia var. alashanensis ultrafine powder could significantly prolong the mice writhing latency (P<0.05) and reduce writhing times(P<0.05), with equivalent efficacy at the same dose.Aquilariae Lignum Resinatum ultrafine powder and S.pinnatifolia var. alashanensis ultrafine powder could evidently prolong the sleep time of mice with threshold doses of pentobarbital sodium (P< 0.05), showing equivalent efficacy at the same dose. Conclusion: Aquilariae Lignum Resinatum ultrafine powder, fine powder and S.pinnatifolia var. alashanensis ultrafine powder had the effects of anti-myocardial ischemia, anti-hypoxia, analgesia, and sedation. In the same dosage,the effects of Aquilariae Lignum Resinatum ultrafine powder were better than those of the fine powder, but equivalent with those of S.pinnatifolia var. alashanensis ultrafine powder.关键词：Aquilariae Lignum Resinatum;Syringa pinnatifolia var. alashanensis;ultrafine powder;myocardial ischemia;creatine kinase (CK);superoxide dismutase (SOD);lactate dehydrogenase (LDH)6|7|6更新时间：2024-01-04
- 摘要：Objective: To evaluate the intervention effect of different processed products of Bupleuri Radix on heat-induced deficiency heat syndrome rats. Method: Totally 46 healthy male rats were randomly divided into two groups:normal group (6 rats), and model group (40 rats). The model group was intragastrically administered with intragastrically Aconiti Lateralis Radix Praeparata, Zingiberis Rhizoma and Cinnamomi Cortex (20 g·kg-1) to establish the internal heat model. After the successful modeling, they were randomly divided into normal group, model group, positive group (Zhibai Dihuangwan, 0.5 g·kg-1), raw Bupleuri Radix group, Turtle blood Bupleuri Radix group, turtle blood combined with Bupleuri Radix group, wine Bupleuri Radix group, and vinegar Bupleuri Radix group. The dosage of each processed product was 3.15 g·kg-1. In the normal group, rats were administered with the same amount of double-distilled water once a day for 11 days. The general conditions and metabolic cage index of rats were measured, and the radix bupleuri basal metabolic changes, D-xylose in serum, triiodothy ronine (T3), thy roxine (T4), cAMP, cGMP content, cortisol, lactic acid (LD), lactate dehydrogenase (LDH), activity of adenosine triphosphate(ATP) enzyme content and organ indexes of rats were compared. Result: Compared with the normal group, the body weight of rats in the model group increased slowly, the rectal temperature, water intake, urine volume and body fluid consumption elevated, and food intake and D-xylose content decreased; cAMP, cGMP content, LD in serum, LDH activity, adenosine triphosphate activity, T3 and T4 content increased (P<0.05). This indicated that the internal heat model was successfully established (P<0.05). Compared with the model group, the D-xylose in different processed product groups and positive drug group increased (P<0.05); the levels of cAMP and cGMP decreased (P<0.05); the levels of lactate and lactate dehydrogenase increase and the levels of ATP, T3, T4 in each group decreased (P<0.05). Conclusion: Bupleuri Radix's different processed products can make the physiological indicators of the internal heat induced by traditional Chinese medicine in rats to return to normal, indicating a therapeutic effect on the internal heat syndrome in rats. However, the effects of different processed products vary on different physiological indexes. Therefore, different processed products have different degrees of intervention on the internal heat of rats.关键词：Bupleuri Radix processed product;heat syndrome due to insufficiency;D-xylose;serum triiodothy ronine;thyroxine;cyclic nucleotide;lactate;lactate dehydrogenase6|7|4更新时间：2024-01-04
- 摘要：Objective: To study the intervention effect of Bushen Jiangzhuoyin on insulin resistance of nonalcoholic fatty liver disease, in order to explore it's effect on cytokines and kinases in insulin signaling pathway. Method: Totally 60 SD rats were randomly divided into blank group, model group, low-dose, middle-dose and high-dose Bushen Jiangzhuoyin groups (10, 15, 20 g·kg-1) and silybin meglumine group (19 g·kg-1). The blank group was fed with normal diet, the other groups were fed with high-fat diet and given normal saline or the corresponding dose of medicines. All of the rats were put to death after 12 weeks, the levels of total cholesterol(TC), triglyceride(TG), alanine aminotransferase(ALT),aspartate aminotransferase(AST), fasting blood glucose(FBG), insulin(INS), insulin resistance index(IRI), insulin sensitivity index(ISI) in serum and adiponectin(ADP), free fatty acid(FFA), tumor necrosis factor-α(TNF-α), superoxide dismutase(SOD), malondialdehyde(MDA) in liver were detected; HE staining and oil red O staining were used to detect the degree of fatty degeneration of liver tissue;the expression levels of c-Jun amino terminal kinase1(JNK1), insulin receptor α(IRα), phosphorylated insulin receptor substrate 1 serine 307(p-IRS-1) were detected by Western blot method. Result: Compared with blank group, model group showed significant increases in levels of TG, TC, ALT, AST, FBG, INS, IRI, TNF-α, FFA and MDA, and JNK1, p-JNK and p-IRS-1 protein expressions, and significant decreases in ISI, ADP, SOD levels (P<0.01); compared with model group, low-dose, middle-dose and high-dose Bushen Jiangzhuoyin groups showed different degrees of reduction in rat serum TG, TC, ALT, AST, FBG, INS, IRI, TNF-α, FFA, MDA levels, and JNK1, p-JNK, p-IRS-1 protein expressions (P<0.05, P<0.01), and rise in ISI, ADP, SOD levels (P<0.05, P<0.01); compared with silybin meglumine group, high-dose Bushen Jiangzhuoyin group showed a significant efficacy (P<0.01). Conclusion: Bushen Jiangzhuoyin can improve IR in NAFLD rats, and it's mechanism may be related to the increase of ADP content, the reduction of oxidative stress, the inhibition of JNK signaling pathway and the enhancement of IRS activity.关键词：nonalcoholic fatty liver disease;insulin resistance;adiponectin;c-Jun N-terminal kinase;insulin signaling pathway;Bushen Jiangzhuoyin9|6|9更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of Qingre Huatan Jiedu formula on lung tissue in rats with cerebral ischemia-reperfusion injury and explore the underlying molecular mechanisms. Method: SD rats were divided into shame group, model group, positive control group (PC group, nimodipine 200 mg·kg-1), low-dose Qingre Huatan Jiedu formula group (LT group, 100 mg·kg-1) and high-dose Qingre Huatan Jiedu formula group (HT group, 200 mg·kg-1), with 10 rats in each group. Except for sham group, modified 4 artery occlusion method was used to prepare the global cerebral ischemia reperfusion injury model in rats. After modeling, the drug was given for 3 d. Lung wet/dry weight (W/D) analysis was made. htoxylin easin(HE) staining was performed for histopathological analysis; the levels of interleukin(IL)-1β and IL-18 in bronchoalveolar lavage fluid (BALF) were detected by enzyme linked immunosorbent assay (ELISA); pulmonary myeloperoxidase kit assay was used to detect myeloperoxidase(MPO) activity;immunohistochemical analysis was performed for nucleotide-binding domain-like receptor 3(NLRP3) positive cells; Caspase-1 colorimetric assay kit was applied in detecting Caspase-1 activity; Real-time PCR and Western blot were used to detect the mRNA and protein expressions of NLRP3, ASC and Caspase-1.Co-immunoprecipitation was used to detect thioredoxon interaction protein(TXNIP)-NLRP3 protein expression. Result: Compared with shame group, lung injury score, W/D ratio of lung, IL-1β and IL-18 levels in BALF, total number of cells and polymorphonuclear leukocytes and MPO activity in model group were significantly increased (P<0.05). Compared with model group, lung injury score, W/D ratio of lung, IL-1β and IL-18 levels in BALF, total cell and polymorphonuclear leukocyte and MPO activity in PC group, LT group and HT group were significantly decreased (P<0.05). Compared with shame group, the relative expression of NLRP3 positive cells, Caspase-1 activity and NLRP3/Asc/Caspase-1 in model group lung tissue were significantly increased (P<0.05). Compared with model group, the expression of NLRP3 positive cells, Caspase-1 activity and NLRP3/Asc/Caspase-1 in PC group, LT group and HT group were significantly decreased (P<0.05). Compared with the shame group, the ROS production and TXNIP protein expression in model group were significantly increased (P<0.05). Compared with model group, ROS production and TXNIP protein expression in PC group, LT group and HT group were significantly decreased (P<0.05). TXNIP-NLRP3 co-precipitation was increased in lungs of the model group compared with the shame group. Compared with model group, the TXNIP-NLRP3 co-precipitation in PC group, LT group and HT group decreased. Conclusion: Qingre Huatanjiedu formula can inhibit the production of ROS, block the TXNIP-NLRP3 inflammatory pathway, reduce the infiltration of inflammatory cells in lung tissue, and alleviate the inflammatory lung injury induced by global cerebral ischemia-reperfusion in rats.关键词：cerebral ischemia-reperfusion;Qingre Huatan Jiedu formula;lung injury;nucleotide-binding domain-like receptor 3(NLRP3);thioredoxin-interacting protein(TXNIP)4|6|3更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of sodium ferulate (SF) on the proliferation, activation and collagen synthesis of rat hepatic stellate cells (HSC-T6) induced by acetaldehyde in vitro and explore its mechanism. Method: HSC-T6 cells were cultured in vitro to establish acetaldehyde-induced HSC-T6 proliferation models. The rats were divided into blank group, acetaldehyde group, colchicine group (2.5 μmol·L-1), and SF groups (1, 10, 25, 50, 100, 200, 400 μmol·L-1). HSC-T6 proliferation was measured by MTS method and the appropriate SF concentration was screened. The effect of SF (400, 200, 100 μmol·L-1) on the expression of α-smooth muscle actin (α-SMA) in HSC-T6 cells induced by acetaldehyde was detected by Western blot; type I and type Ⅲ collagen concentrations were detected by Enzyme-linked immunosorbent assay (ELISA); the Hydroxyproline (Hyp) content was detected by hydrolysis method; mRNA expression levels of transforming growth factor-β1 (TGF-β1), signal transducers Smad2, Smad3, Smad4, Smad7, Matrix metallo proteinase 1 (MMP-1) and tissue inhibitors of metallo proteinase-1 (TIMP-1) were detected by real-time fluorescence quantitative polymerase chain reaction (Real-time PCR). Result: As compared with the blank group, 200 μmol·L-1 acetaldehyde significantly induced HSC-T6 proliferation in vitro (P<0.01). As compared with the blank group, the proliferation of HSC-T6 in model group was significantly increased (P<0.01), and the contents of α-SMA, type I, type Ⅲ collagen and Hyp were significantly increased (P<0.01), the mRNA expression levels of TGF-β1, Smad2, Smad3, Smad4 and Smad7 as well as mRNA expression levels of MMP-1 and TIMP-1 were significantly up-regulated (P<0.01). As compared with the model group, SF at concentrations of 400, 200, 100 μmol·L-1 significantly inhibited acetaldehyde-induced HSC-T6 proliferation (P<0.05, P<0.01), significantly decreased α-SMA, type I and type Ⅲ collagen and Hyp contents (P<0.05, P<0.01), down-regulated the mRNA expression of TGF-β1, Smad2, Smad3 and Smad4 (P <0.05, P<0.01), up-regulated the mRNA expression of Smad7 mRNA (P<0.05,P<0.01) and down-regulated the mRNA expression of TIMP-1 (P<0.01). Conclusion: SF can inhibit proliferation, activation and collagen secretion of HSC-T6 induced by acetaldehyde in vitro by regulating TGF-β1/Smads and MMP-1/TIMP-1 signaling pathways.关键词：sodium ferulate;hepatic stellate cells(HSC-T6);acetaldehyde;proliferation;activation;collagen4|6|2更新时间：2024-01-04
- 摘要：Objective: To investigate the effect and mechanism of Bushen Huoxue formula on hippocampal cells apoptosis in rats with vascular dementia (VD). Method: Totally 75 male SD rats were screened in Morris Water-maze screening, and then randomly divided into the sham-operated group, model group, Nimodipine group, Bushen Huoxue formula high and low dose (10.14, 5.07 g·kg-1·d-1) groups. Except the rats in the sham-operated group, all the other four groups received two-vessel occlusion (2-VO) method to establish VD models. The rats in the low-dose and high-dose Bushen Huoxue formula groups and the Nimodipine group received drugs Bushen Huoxue formula tablets and Nimodipine tablets respectively for 30 days. After treatment, all rats were tested by Morris Water-maze; brain hippocampal cells apoptosis was detected by TUNEL method and the expression levels of rat hippocampal extracellular signal-regulated kinase 2 (ERK2) and cAMP response element binding protein (CREB) were detected by immunohistochemistry and Western blot. Result: As compared with sham-operated group, the time of escape latency was significantly increased (P<0.05); the number of passing platform and the stayed time in the former platform quadrant were both decreased (P<0.01); the integral absorbance (IA) of hippocampal cells apoptosis was significantly increased, and the average grey value was decreased significantly (P<0.01); IA of hippocampal ERK2 and CREB (immunohistochemical) expression was significantly decreased, and the average grey value was significantly increased (P<0.01); and the protein expression level (Western blot) was significantly decreased in model group (P<0.01). As compared with the model group, Bushen Huoxue formula improved the learning and memory ability of rats, decreased the apoptosis IA (P<0.01), increased the average grey value (P<0.01), and increased the IA and protein expression of ERK2 and CREB in hippocampal tissues(P<0.05,P<0.01). Conclusion: Bushen Huoxue formula may inhibit apoptosis of hippocampal cells and improve the learning and memory ability of VD rats through regulating ERK/CREB signaling pathways in hippocampal tissues of rat.关键词：Bushen Huoxue formula;vascular dementia;cell apoptosis;extracellular signal-regulated kinase 2(ERK2);cAMP response element binding protein(CREB)8|5|11更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of ultra-filtration extraction from Angelicae Sinensis Radix and Hedysari Radix on the cell proliferation and collagen deposition of myocardial fibroblasts induced by radiation. Method: Rat myocardial fibroblasts were separated and cultured by cell adhesion. The cell proliferation was determined by thiazolyl blue tetrazolium bromide (MTT) method, and the collagen- Ⅰ (COL- Ⅰ) of secretion was determined by enzyme-linked immuno sorbent assay (ELISA) method after ultra-filtration extraction from Angelicae Sinensis Radix and Hedysari Radix or radiation to myocardial fibroblasts. The changes of reactive oxygen species (ROS) were determined by flow cytometry with indicator 2',7'-dichloride fluorescent yellow double acetate (DCFH-DA) staining. The mRNA expressions of COL- Ⅰ and transforming growth factor-beta (TGF-β) were determined by Real-time polymerase chain reaction (PCR), and the expressions of relative proteins were determined by Western blot. Result: The rising cellular ROS promoted cell proliferation and collagen deposition of myocardial fibroblasts induced by low-dose radiation (0.5 Gy), which however was inhibited through ultra-filtration extraction from Angelicae Sinensis Radix and Hedysari Radix by promoting nuclear translocation of nuclear factor E2 related factor 2 (Nrf2) and eliminating ROS. The mRNA expressions of COL- Ⅰ and TGF-β were both inhibited by ultra-filtration extraction from Angelicae Sinensis Radix and Hedysari Radix. Conclusion: Ultra-filtration extraction from Angelicae Sinensis Radix and Hedysari Radix could inhibit the cell proliferation and collagen deposition of myocardial fibroblasts induced by low-dose radiation.关键词：Angelicae Sinensis Radix;Hedysari Radix;myocardial fibroblast;radiation;proliferation;collagen6|7|4更新时间：2024-01-04
- 摘要：Objective: To explore the function and possible mechanism of berberine on colon cancer cell SW620 based on Kirsten rat sarcoma viral oncogene homolog(KRAS) promoter G-quadruplex target, by investigating its effect on colon cancer cells proliferation and whether berberine could inhibit mRNA expression of KRAS in colon cancer cells through regulating its promoter G-quadruplex. Method: The inhibition rate of berberine on SW620 cell lines proliferation was determined by using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. The binding capacity of KRAS G-quadruplex DNA with berberine was detected by methods of fluorescence spectra, and the anionic quenching experiment, and fluorescence anisotropy and fluorescence polarization experiment were used to investigate their binding mode. The effect of berberine on conformation of KRAS G-quadruplex was studied by using circular dichroism spectra; the effect of berberine on thermal stability of KRAS G-quadruplex was studied by using circular dichroism spectra variable-temperature experiment, and the effect of berberine on KRAS genetic transcription of SW620 cells was detected by real-time quantitative polymerase chain reaction (Real-time PCR). Result: Berberine can inhibit the proliferation of SW620 cells in a dose dependent manner. KRAS G-quadruplex DNA had a stronger binding capacity with berberine at a stoichiometric ratio of about 1:1, with a binding constant of (0.93±0.21)×106 L·mol-1, under a binding mode of complex patterns of terminal stacking and groove binding. Berberine can keep the parallel conformation and thermostability of KRAS G-quadruplex, and 100 μmol·L-1 berberine can inhibit the mRNA expression of KRAS in SW620 cells. Conclusion: Berberine can bind with KRAS promoter G-quadruplex DNA and keep the thermostability of its parallel conformation, and may down-regulate KRAS genetic transcription of SW620 cells by affecting its G-quadruplex structure, which may be one of the mechanisms for inhibiting the proliferation of cells. KRAS promoter G-quadruplex structure may become a new target in colon cancer therapy.关键词：Kirsten rat sarcoma viral oncogene homolog(KRAS)promoter;G-quadruplex;berberine;colon cancer;anti-tumor mechanism5|6|2更新时间：2024-01-04
- 摘要：Objective: To investigate the changes of endogenous metabolites in urine of nephrotic syndrome rats treated by compatibility of Tripterygii Wilfordii Radix et Rhizoma and Glycyrrhizae Radix et Rhizoma,and to explore its mechanism by urine metabonomics. Method: Rats were randomly divided into 4 groups,including the blank group,the model group,the Tripterygii Wilfordii Radix et Rhizoma (TPW) group and the compatibility of Tripterygii Wilfordii Radix et Rhizoma and Glycyrrhizae Radix et Rhizoma (TPW-GLY) group.All urine samples were determined by UPLC-Q-TOF-MS.Data was analyzed by principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA). Result: The urine metabolite profiles in 4 groups were separated obviously.Twenty-nine biomarkers with significant difference were identified between the blank group and the model group,and 14 biomarkers were recovered by the administration of TPW-GLY,and 11 biomarkers were recovered by the administration of TPW.There were 7 biomarkers between TPW group and TPW-GLY group as N-acetylglutamic acid,dihydroxycoprostanoic acid,nicotinuric acid,triglyceride(16:0/16:0/20:4),12-ketodeoxycholic acid,ubiquinone Q2,1,3,7-trimethyluric acid. Conclusion: After compatibility with Glycyrrhizae Radix et Rhizoma,Tripterygii Wilfordii Radix et Rhizoma may take effect on nephrotic syndrome rats through the regulation of amino acid metabolism,bile acid metabolism,fatty acid and fat metabolism,energy metabolism,purine metabolism and other processes.关键词：Tripterygii Wilfordii Radix et Rhizoma;Glycyrrhizae Radix et Rhizoma;compatibility;nephrotic syndrome;metabonomics;biomarkers;metabolic pathway6|7|8更新时间：2024-01-04
- 摘要：Objective: To study the antibacterial and anti-inflammatory effects of crude extract of Dalbergia pinnata and D. benthamii,two kinds of Dalbergia plants. Method: Two fold dilution method was used to investigate the antimicrobial activity of crude extracts from two plants against Staphylococcus epidermidis, Propionibacterium acnes and S. aureus. Mice were divided into 8 groups randomly, including blank group, positive group, D. benthamii and D. pinnata low dose group, middle dose group and high dose group (0.85, 1.7, 2.6 g·kg-1), 8 rats in each group. Ear swelling induced by dimethylbenzene, paw swelling induced by egg white and acne model in mice were used to investigate the anti-inflammatory effect of crude extract of two Dalbergia plants. Result: Two Dalbergia plants had antibacterial effects against S. epidermidis, P. acnes, and S. aureus, with minimum inhibitory concentration (MIC) of 5, 10, 40 g·L-1 for D. benthamii were and 2.5, 2.5, 20 g·L-1 for D. pinnata respectively. The middle and high dose of two Dalbergia plants could significantly relieve ear-swelling caused by dimethylbenzene in mice (P<0.05,P<0.01). The high dose of D. pinnata and the middle and high dose of D. benthamii could significantly inhibit paw swelling induced by egg white within 3, 4, 6 hours (P<0.01). The middle dose of D. pinnata and the high and middle dose of D. benthamii could significantly alleviate ear swelling induced by mixed bacteria solution (P<0.01). Conclusion: Both D. pinnata and D. benthamii have significant antibacterial and anti-inflammatory effects.关键词：Dalbergia pinnata;D. benthamii;Dalbergia;antibacterial;anti-inflammatory4|6|3更新时间：2024-01-04
- 摘要：Objective: To investigate the distribution regularity of traditional Chinese medicine (TCM) syndrome of IgA nephropathy and the predictive value of microRNA(miR) on IgA nephropathy. Method: Totally 109 clinical cases diagnosed as IgA nephropathy by renal puncture were collected. According to the diagnostic criteria of TCM syndromes, they were divided into four groups:lung and kidney deficiency group, spleen and kidney deficiency group, liver and kidney deficiency group, and Qi and Yin deficiency group. The relationship between TCM syndrome distribution and clinical indexes was analyzed. Among the patients with the highest syndromes, 10 cases were randomly selected, with 10 healthy people as controls, and the expression of miR was detected by Real-time PCR. Result: Among the five syndromes, lung-kidney-Qi deficiency was the lightest clinical manifestation, with the lowest pathological grade; Spleen-kidney-Yang deficiency was the heaviest clinical manifestation, with the highest pathological grade; the glomerular filtration rate was lower than the other four types, whereas the 24-hour urine protein, serum creatinine were higher than the other four types. Among them, Qi and Yin deficiency syndrome had the highest frequency in IgA patients, with a total of 49 cases. The serum levels of miR141 and miR146a were higher than those in normal group, while miR205, miR155 and miR192 were lower than normal group. Conclusion: The pathological changes of IgA nephropathy and the changes of clinical physical and chemical indexes aggravated to a certain extent. The pathogenesis evolution of TCM syndromes is that the pathogenesis of the syndromes of the kidney and kidney is Qi and kidney deficiency-spleen and kidney Qi deficiency-liver and kidney Qi deficiency-Qi and Yin deficiency-spleen and kidney Yang deficiency. The difference of miR expression is closely related to the severity of IgA nephropathy and the progression of the disease, which provides a new basis for the pathogenesis and diagnosis of IgA nephropathy.关键词：IgA nephropathy;syndrome differentiation;clinical indicators;microRNA(miR)5|6|0更新时间：2024-01-04
- 摘要：Objective: To investigate the therapeutic targets of Da Chaihutang in the patients with unstable angina pectoris (UA) of coronary heart disease and predict the pathway in the treatment of angina pectoris. Method: This prospectivestudy had a total of 110 patients admitted in Cardiovascular Department 1 in the Affiliated Hospitalof Liaoning University of Traditional Chinese Medicine between September 2016 to September 2017.The patients were arranged in order according to their order of admission, and were divided into treatment group and control group by random number table method, 55 cases in each group. Basic western medicine was given in control group, and the basic medicine + Da Chaihutang was given in treatment group. Then the expression levels of Ghrelin and Obestatin and the composition of intestinal flora were analyzed in this study. Result: After basic treatment combined with Da Chaihutang, the total effective rate in patients with coronary heart disease unstable angina was increased significantly (P< 0.05).Results suggested that Da Chaihutang can significantly improve the clinical symptoms in patients with UA, and the further determination of Obestatin and Ghrelin expression levels showed that Da Chaihutang treatment promoted the up-regulation of Ghrelin and down-regulation of Obestatin in UA patients. The results of intestinal flora suggested that Da Chaihutang could change the compositions of Bacteroidetes and Firmicutes in UA patients. Conclusion: Da Chaihutang can significantly affect the expression of serum Ghrelin and Obestatin in UA patients, regulate the intestinal flora of UA patients, and increase the species and abundance of beneficial bacteria.关键词：unstable angina;Ghrelin;Obestatin;high-throughput sequencing;intestinal flora7|7|9更新时间：2024-01-04
- 摘要：Objective: To explore the efficacy and safety of Shugan Lidan Tang combined ursodeoxycholic acid in treatment of early and mid-term primary biliary cirrhosis hygroscopic blood stasis syndrome. Method: A total of 171 cases primary biliary cirrhosis patients were randomly divided into the traditional Chinese medicine(TCM) group, the chemical medicine group and the combined group, with 57 cases in each group. The TCM group was treated with Shugan Lidan Tang, ursodesoxycholic acid was applied in the chemical medicine group, Shugan Lidan Tang and ursodesoxycholic acid was provided to the combined group. A course of treatment was 48 weeks. The changes of clinical symptom, serum fibrosis, liver function and immunological indicators were observed before and after treatment. To compare the complete response and safety index in three groups. Result: The total response rate in combined group in the 24th, 48th weeks was higher than that of the TCM group and the combined group (P<0.05). There was no significant difference between the TCM group and the western medicine group. The clinical symptoms, serum fibrosis, liver function, immunoglobulin G (IgG), immunoglobulin M (IgM), immunoglobulin A (IgA), anti-mitochondrial antibody (AMA) were superior to those in TCM group and the chemical medicine groups in the 48th week (P<0.05). The order of the groups in terms of adverse reactions and blood and urine routine was the TCM groupP<0.05). Conclusion: Shugan Lidan Tang has the effect in the treatment of early and mid-term primary biliary cirrhosis. Compared with ursodesoxycholic acid, there is no obvious difference, with a better safety index. Shugan Lidan Tang combined with ursodeoxy-cholic acid has a remarkable efficacy in treating early and mid-term primary biliary cirrhosis hygroscopic blood stasis syndrome.关键词：Shugan Lidan Tang;primary biliary cirrhosis;ursodesoxy cholic acid;hygroscopic blood stasis syndrome;anti-mitochondrial antibody6|6|1更新时间：2024-01-04
- 摘要：Objective: To observe the efficacy of Dizhen grain in treating menopausal syndrome (MPS) with internal heat due to yin deficiency, in order to investigate its effect on neuro-endocrine. Method: Totally 130 cases with MPS were selected and randomly divided into control group (65 cases) and treatment group (65 cases). Control group was orally treated with Oryzanol tablets, 2 tablets/time, tid. In addition to the therapy of control group, treatment group was given Dizhen grain, 1 bag/time, tid. Both groups were observed for 3 months. Scores of Kupperman (KI), Menopause-specific quality of life (MENQOL) and efficacy were compared between the two groups. Serum levels of interleukin (IL)-6, 5-hydroxytryptamine (HT), corticosteroid releasing hormone (CRH), corticosteroid (ACTH), corticosterone (CORT), gonadotropin releasing hormone (GnRH), norepinephrine (NE) and serum estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH) were detected in both groups. Plasma level of β-endorphin (β-EP) was measured in the two groups. Result: After treatment, scores of KI and indexes of MENQOL of treatment group were obviously lower than those of control group (P<0.01). The total effective rate of treatment group was 98.46%, which was superior to 87.69%of control group (χ2=4.298, P<0.05). After treatment, serum levels of IL-6, CRH, ACTH, CORT, GnRH, NE of treatment group were remarkably lower than those of control group, while 5-HT was higher (P<0.01). Plasma level of β-EP of treatment group was evidently higher than that of control group (P<0.01). After treatment, serum level of E2 of treatment group was remarkably higher than control group, while FSH and LH were lower (P<0.01). Conclusion: In addition to the therapy of control group, Dizhen grain has a significant effect in treating MPS with syndrome of internal heat due to Yin deficiency, which may be related to the regulation of neuro-endocrine.关键词：Dizhen grain;menopausal syndrome;syndrome of internal heat due to Yin deficiency;neuro-endocrine6|6|2更新时间：2024-01-04
- 摘要：Objective: To discuss the efficacy of Shenfutang combined with Huanglian Ejiao Tang in improving the postoperative cognitive dysfunction (POCD) of patients with postoperative delirium (POD) after orthopedics operation, and its effect on levels of neuron specific enolase (NSE), S-100β protein, inflammatory response and oxidative stress injury. Method: One hundred and four patients were randomly divided into control (52 cases) group and observation group (52 cases) according to the ratio of 1 to 1.Patients in control group got haloperidol tablets, 4-20 mg/days, 3 take. In addition to the therapy for control groups, patients in observation group were also given modified Shenfutang combined with Huanglian Ejiao Tang, 1 dose/day, for 10 successive days. And the mini-mental state examination (MMSE), delirium rating scale (DRS), disappearance time of postoperative delirium and delirium rating scale (DRS-R-98) were evaluated. Before and after treatment, levels of NSE, S-100β, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), superoxide dismutase (SOD) and malondialdehyde (MDA) were detected. The clinical efficacy was evaluated by the scale of clinical global impression (CGI-GI). Result: The total effective rate in observation group was 88.64%, which was higher than 69.23%in control group (χ2=5.765, P<0.05). And at the 5th and 10th day after the treatment, scores of CAM and DRS-R-98 in observation group were lower than those in control group, and MMSE was higher than that in control group (P<0.05, P<0.01). CAM and DRS-R-98 in observation group reduced more significantly than those in control group, but MMSE increased more significantly than that in control group (P<0.01). Scores of ADL and traditional Chinese medicine(TCM) syndrome in observation group were both lower than those in control group (P<0.01). The average disappearance time of postoperative cognitive dysfunction in observation group was shorter than that in control group (P<0.01). And levels of NSE, S-100β, IL-6, TNF-α and MDA in observation group were lower than those in control group, while level of SOD was higher (P<0.01). Conclusion: Modified Shenfutang combined with Huanglian Ejiao Tang can promote the recovery of POD, improve the cognitive function, relieve symptoms, reduce oxidative stress and injury caused by inflammatory reaction, improve the daily living ability, and protect brain nerve cells, and thus is worth clinical application.关键词：orthopaedics;postoperative delirium after operation;postoperative cognitive dysfunction;Shenfutang;Huanglian Ejiao Tang;neuron specific enolase;S-100β protein;inflammatory factor9|6|1更新时间：2024-01-04
- 摘要：Objective: To explore the characteristics of traditional Chinese medicine(TCM) syndromes of the risk of the recurrent ischemic stroke. Method: The cases were selected from January 1st,2014 to December 31th,2015 in the chronic disease prevention and treatment information coordinate platform of the affiliated hospital to Changchun University of Chinese Medicine to start a cross-sectional study.Logistic multiple regression,generalized Boosted model and Wilcoxon rank sum test were used to discuss the influence of TCM syndromes and the syndrome factors on the recurrence of stroke. Result: In this study,3 322 cases were selected,including 1 542 cases of the new ischemic stroke and 1 780 cases of recurrent ischemic stroke.Characteristics of TCM syndromes manifested an important factor affecting the recurrence of ischemic stroke through generalized Boosted model.The result from Wilcoxon rank sum test showed that constituent rates of Qi deficiency and blood stasis syndrome,Yin deficiency and wind formation syndrome,upward disturbance of wind-fire syndrome and phlegm-heat and excessive Fu-viscera syndrome had statistical differences both in new and recurrence group(P<0.05,P<0.01);the constituent rates of phlegm,stasis and Qi deficiency in recurrence ischemic stroke were higher than the first ischemic stroke(P<0.01).Furthermore,Logistic multiple regression analysis was used to find that the performance of obstruction of collateral caused by windphlegm syndrome,Qi deficiency and blood stasis syndrome,phlegm-heat and excessive Fu-viscera syndrome,orifices confused by phlegm syndrome and phlegm-heat internal block syndrome may be the risk factor for the recurrence of ischemic stroke(P<0.05,P<0.01).All wind,fire,phlegm,stasis,Qi deficiency and Yin deficiency may also be a risk factor for the recurrence of ischemic stroke(P<0.05,P<0.01). Conclusion: Recurrence group of ischemic stroke manifests the characteristics of TCM syndromes.It indicates that the factors of the characteristics of TCM syndromes should be paid attention to in the stroke secondary prevention.It's necessary to be verified by means of evidence-based medicine and clinical practice.关键词：ischemic stroke;risk of recurrence;traditional Chinese medicine syndromes;multivariate analysis;cross-sectional study7|6|7更新时间：2024-01-04
- 摘要：Objective: To explore the mechanism and principle of Gegen Qinlian Tang in the treatment of type 2 diabetes by systematic pharmacology. Method: The effective components and related target proteins in Gegen Qinlian Tang were selected by using the traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP); the network visualization software Cytoscape 3.5.1 was used to construct the effective component-target interaction network of Gegen Qinlian Tang for topological analysis. Protein and protein interaction network (PPI) was constructed and analyzed with STRING 10.5 online database. After the gene names of target proteins were obtained from Uniprot database, kyoto encyctopedia of genes and genomes(KEGG) pathway analysis was conducted by using David database to explore the mechanism and principle of Gegen Qinlian Tang in treating type 2 diabetes. Result: The results showed that the core compounds of Gegen Qinlian Tang included quercetin, kaempferol, beta-sitosteroll, Prostaglandin G/H synthase 2, etc.; PPI network diagram showed that the key proteins in the effective constituents of Gegen Qinlian Tang mainly included c-jun N-terminal kinase (JNK), tumor necrosis factor (TNF), fos protein (FOS), mitogen activated protein kinase 3 (MAPK3), etc. KEGG results showed that the main effect of Gegen Qinlian Tang was associated with TNF signaling pathway and MAPK signaling pathway signal pathway. Conclusion: Gegen Qinlian Tang mainly plays its anti-inflammatory effect, so as to achieve the effect of treating type 2 diabetes through multiple targets and multiple signaling pathways.关键词：Gegen Qinlian Tang;type 2 diabetes;pathway;mechanism;target9|6|6更新时间：2024-01-04
- 摘要：Objective: To analyze and summarize the prescription principles of traditional Chinese medicine (TCM) for treating intestinal flora alteration during 1995-2016 by using TCM Inheritance Support System, and make predictions of new formulations according to the composing principles. Method: Related papers on TCM prescriptions for treating intestinal flora alteration were collected from China national knowledge infrastructure (CNKI), and then TCM Inheritance Support System was used to establish the prescription database and analyze the composing principles of herbs in the prescriptions. Result: Totally 148 papers on the prescriptions for treating intestinal flora alteration were screened to achieve 32 commonly used herbs, 7 common composing principles, 16 core combinations of new prescriptions and 8 new prescriptions. Conclusion: Analysis shows that the drugs for intestinal flora alteration are mainly those for regulating spleen and stomach, eliminating dampness and alleviating water retention. The new prescriptions obtained by cluster analysis have similar treatment effect with the common prescriptions, such as Shenling Baizhu San, Buzhong Yiqitang, Jianpi Shenshitang, for intestinal flora alteration, but the new prescriptions, also have the herbs with regulating Qi and relieving liver functions to achieve the effects of regulating liver and Qi as well as tonifying the spleen and stomach. Compatibility combination conforms to the main clinical treatment ideas of invigorating Qi and tonifying spleen, excreting dampness and reducing diarrhea, helpful to guide clinical practice, and providing certain basis for clinical development and use of new drugs.关键词：intestinal flora alteration;Traditional Chinese Medicine Inheritance Support System;prescription principles;couplet medicines;medication mode7|6|15更新时间：2024-01-04
- 摘要：There are over 150 species in Zygophyllum genus in the world,distributed mainly in Asia,Australia,Africa and Mediterranean coast. These species have the properties of drought resistance,wind and sand resistance,and saline-alkali resistance. The seeds are easy to germinate everywhere, with high adaptability. The researches on this genus mainly focused on the pharmacological activities of extracts or fractions as well as the separation and identification of chemical constituents. Z. aegyptium,Z. album,Z. atriplicoides,Z. berenicense,Z. coccineum,Z. cornutum,Z. decumbens,Z. ducembens, Z. dumosum,Z. eichwaldii,Z. eurypterum,Z. fabago,Z. gaetulum,Z. geslini,Z. melongena,Z. oxianum,Z. propinquum and Z. simplex have been used as herbal medicines or have shown biological activities in studies. Some of them have been used in folk of Asian and African countries to treat the diseases such as diabetes,hyperlipidemia,hypertension,obesity,diarrhea and spasticity. Some of them have shown hypoglycemic,antihypertensive,hypolipidemic,antispasmodic, antipyretic,anti-diarrhea,inhibiting enzymes,anti-oxidation,anti-inflammatory,antibacterial,anti-cancer and other pharmacological activities. Zygophyllum genus can be used for prevention and treatment of metabolic syndrome;the plants have a variety of biological activities. Further researches on this genus are needed in the following aspects:①bioactivity-guided isolation of the biological compounds;②clarification of molecular mechanisms;③ content analysis of biological compounds by using HPLC,GC,and LC-MS etc. The saponins,flavonoids and other main compounds isolated and identified from Zygophyllum plants as well as their known pharmacological activities were reviewed in this paper.关键词：Zygophyllum genus;compounds;saponins;flavonoids;alkaloids and others6|6|0更新时间：2024-01-04
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