最新刊期
卷 24 ， 期 13 ， 2018
- 摘要：Objective: To investigate the correlation of contents of iridoid glycosides and diterpene pigments as well as the apparent color of Gardeniae Fructus during the processing and provide the basis for regulating the processing technology of this herb. Method: During the processing of Gardeniae Fructus,the correlation analysis of multi-source data was carried out by Pearson method,which including change regularity of chromatic value measured by color analyzer and content variation of total iridoid glycosides and total diterpene pigments measured by UV. Result: The variation tendency of chromatic value[L*(lightness value),a*(red and green component value),b*(yellow and blue component value)]was negatively correlated with the content of total iridoid glycosides and positively correlated with the content of total diterpene pigments in the process of Gardeniae Fructus processing. L* was most correlated with the content of total diterpene pigments in process of stir-baked Gardeniae Fructus, b* was most correlated with the content of total diterpene pigments in process of carbonized Gardeniae Fructus. Conclusion: The color and pigments can be considered as the control and monitoring index in the process of Gardeniae Fructus processing.关键词：Gardeniae Fructus;processing;apparent color;iridoid glycosides;pigment;crocin-Ⅰ;geniposide7|6|18更新时间：2024-01-04
- 摘要：Objective: To explore the influence of Rehmanniae Radix Praeparata decoction processed with different techniques on the ovulation function of female rats,and to provide reference for the processing and clinical use of this decoction pieces. Method: Steamed method (one steaming and one drying) and traditional method (nine steaming and nine drying) were used to prepared Rehmanniae Radix Praeparata,respectively.Female adult rats with a normal estrus cycle were selected as study subjects,blank group,steaming group and traditional group were set up.The effects of two Rehmanniae Radix Praeparata decoctions on the estrous cycle,ovarian tissue morphology and serum hormone levels in female rats were observed.The doses in the steaming group and the traditional group were 3.6 g·kg-1.The HPLC fingerprints of Rehmanniae Radix Praeparata was established,fingerprints and pharmacodynamic indexes were used to investigate effect of Rehmanniae Radix Praeparata on ovulation of female rats. Result: Compared with the blank group,serum estradiol and progesterone levels in the steaming group were significantly decreased(P<0.05),the estrous cycle was significantly prolonged(P<0.05),and the number of atresia follicles in the ovary was significantly increased(P<0.05);the change of each index in the traditional group was not obvious.Verbascoside and effect of inhibiting ovulation of Rehmanniae Radix Praeparata processed steamed method in rats positively correlated;peak 3 and 5 in HPLC fingerprints of Rehmanniae Radix Praeparata processed with traditional method were negatively correlated with inhibition of ovulation. Conclusion: Rehmanniae Radix Praeparata processed by steamed method has a certain inhibitory effect on ovulation of normal female rats.Rehmanniae Radix Praeparata processed by traditional method has some advantages in ovulation function.关键词：Rehmanniae Radix Praeparata;processing;inhibition of ovulation;fingerprint;verbascoside;5-hydroxymethylfurfural;estradiol8|6|4更新时间：2024-01-04
- 摘要：Objective: In order to supply theoretical basis for clinical application of Tanreqing solution,in vitro deposition properties of 6 kinds of jet nebulizers were measured and compared. Method: The in vitro deposition properties of 6 kinds of jet nebulizers were determined by BRS2000 respiratory simulator and next generation impactor(NGI). Result: Delivery rates of Boy SX nebulizer with bule insert was 42.51 μg·min-1,it was the fastest among 6 kinds of jet nebulizers.The total delivery quantity of Boy SX nebulizer with red insert was 252.20 μg,it was the highest in 6 kinds of jet nebulizers.Different nebulizer had significant difference on total delivered dose(P<0.05).The amount of residue in inhalation solution cup of Boy SX nebulizer with red insert was the lowest (59.78 μg).The mass median aerodynamic diameter of aerosol measured with 6 kinds of nebulizers ranged from 3.57 μm to 4.98 μm.The mass median aerodynamic diameter and geometric standard deviation of Boy SX nebulizer with red insert were the lowest,but its effective deposition rate of fine particles was the highest. Conclusion: Particle size distribution of Boy SX nebulizer with red insert is the narrowest,and its fine particles have the most effective deposition rate;this nebulizer is most suitable for clinical use of Tanreqing inhalation solution.关键词：jet nebulizers;Tanreqing inhalation solution;in vitro deposition properties;respiratory simulator;next generation impactor;effective deposition rate of fine particles8|6|3更新时间：2024-01-04
- 摘要：Objective: By measuring the color values of Aucklandiae Radix powder and the contents of costunolide,dehydrocostus lactone and volatile oil,the index value of the representative color was correlated with the index value representing the quality,to explore the correlation between contents of active ingredients and the color values, and provide basis for the quality evaluation of Aucklandiae Radix. Method: The color difference values of Aucklandiae Radix powder was measured by colorimeter.The contents of costunolide and dehydrocostus lactone were determined by HPLC,mobile phase was methanol-water(65:35),the detection wavelength was set at 225 nm.The content of volatile oil was determined by steam distillation method.The correlation analysis was carried out by SPSS 21.0 software. Result: There was a negative correlation between L*(representing color shade),b*(representing color red-green direction),E*ab(representing total color difference) and contents of costunolide,dehydrocostus lactone,volatile oil(P<0.01). Conclusion: The color values of Aucklandiae Radix powder has a certain correlation with the content of costunolide,dehydrocostus lactone and volatile oil.The smaller the content of L*,b* and E*ab,the higher the content of three active ingredients.The color is darker,the content of active ingredients in Aucklandiae Radix is higher and its quality is better.The contents of 3 active ingredients in Aucklandiae Radix can be quickly predicted by determining the color difference values,which can provide a new idea for quality evaluation of this herb.关键词：Aucklandiae Radix;color;correlation;costunolide;dehydrocostus lactone;volatile oil;color difference meter6|7|13更新时间：2024-01-04
- 摘要：Objective: To carry out the investigation on Tetracera asiatica,and lay a foundation for assessing and selecting better T. asiatica. Method: Field visit was combined with literature review to investigate the geographical distribution and habitat,biological characteristics,and associated plants of T. asiatica resources. Result: T. asiatica was mainly distributed in Guangdong,Guangxi,Hainan,located at N 18°01'-23°75',E 101°37'-118°05',with altitude between 0-681 m,and at an average elevation of 179 m.There was less and even no distribution beyond this range. Suitable habitat was subtropical evergreen broad-leaved forest and south Asian tropical monsoon evergreen broad-leaved forest,in damp and hot areas of damp valleys or hillside forests or bushes,with humidity of 70% to 91%.The annual average temperature for the growth place was 18.3℃ to 25.4℃,with rainfall of 1 112.1 mm to 2 996.8 mm. Conclusion: The geographical distribution characteristics,biological characteristics and associated plant community types of T. asiatica were preliminarily identified,and the heat factor and humidity were the main limiting indexes for the distribution of T. asiatica. Based on the investigation of germplasm resources and effective composition analysis showed that T. asiatica germplasm resources were abundant, especially in Guangdong and Hongkong,it was well protected and the quality of the medicinal materials was good. At the same time,the prospect for its application was analyzed, providing reference for the protection,development and utilization of T. asiatica resources.关键词：Tetracera asiatica;germplasm resource;investigation;geographical distribution;biological characteristics7|6|1更新时间：2024-01-04
- 摘要：Objective: To screen the potential inhibitor for human epidermal growth factor receptor (EGFR) from four kind compounds including shikonin,acetylshikonin,β-acetoxyisovalerylshikonin and β,β-dimethylacrylshikonin contained in Arnebia euchroma. Method: Through adsorption experiment,the best conditions of the pH and concentration for immobilization of EGFR were determined by using the surface plasmon resonance (SPR) method to construct EGFR biochip. The interactions between EGFR and four compounds were studied on real time after the EGFR immobilization on the chip with PBST (10 mmol·L-1,pH 7.4, including 0.005% tween 20) as the interaction buffer. The kinetic parameters were calculated by software, and the inhibitors were then screened based on the combination with kinetic constant. Result: The optimal immobilization conditions were as follows:with 10 mg·L-1 acetate buffer (10 mmol·L-1,pH 4.5),and was immobilized with a 250 response unit finally. The interaction results showed that only β,β-dimethylacrylshikonin had good interaction with EGFR among them, and other three compositions had no clear response. The kinetic parameters were calculated further,with binding rate constant ka=1.27×104 L·mol-1·s-1,dissociation rate constant kd=2.92×10-2 s-1, dissociation equilibrium constant KD=2.31×10-6 mol·L-1 and Chi square value (Chi2) of 3.25. KD<1×10-5 mol·L-1,suggesting that they had a strong affinity. Conclusion: It was indicated that the β,β-dimethylacrylshikonin could be a potential EGFR inhibitor.关键词：epidermal growth factor receptor(EGFR);Arnebia euchroma;surface plasmon resonance(SPR);β,β-dimethylacrylshikonin6|6|0更新时间：2024-01-04
- 摘要：Objective: To provide theoretical basis for artificial cross breeding of Scutellaria baicalensis by studying the characteristics of stigma receptivity as well as the viability and life-span of pollen. Method: The pollen viability of S. baicalensis was measured by 2,3,5-triphenyltetrazolium chloride (TTC) and iodine-potassium iodide (I2-KI) tests, and the pollen viability under different storage methods, temperatures and duration were detected by I2-KI test. Furthermore, the stigma receptivity was detected by benzidine-H2O2 method. Result: TTC and I2-KI showed the same effectiveness in the detection of pollen viability. The pollen viability of S. baicalensis demonstrated increase first and then decrease with the increase of blooming time. The highest pollen viability was at day 4 after blooming. Among 8:00 to 18:00, pollen viability was over 62%; and at about 12:00, pollen viability was highest (86.58%). The pollen viability was decreased with the extension of storage time, with difference between different storage methods (dry method and wet method). The growth rate of stigma tended to be stable at day 5 after blooming, and the stigma receptivity was strongest at day 6. Conclusion: The best time of artificial pollination of S. baicalensis is day 5-7 after blooming, and the pollen at day 4 after blooming could be used to pollinate the stigma at day 5-7 after blooming.关键词：Scutellaria baicalensis;pollen viability;stigma receptivity;heat-clearing and damp-drying;purging internal heat and removing toxin6|7|2更新时间：2024-01-04
- 摘要：Objective: To supplement and improve the quality control method hrough a bioassay test about ossotide for injection on inhibiting the proliferation of monocyte-macrophages(THP-1). Method: The biological activity of ossotide for injection was investigated by its inhibitory effect on THP-1 proliferation in vitro with CCK-8 assay. A01160410 batch of ossotide for injection was used as standard control substanceunder the potency determination. Result: The ossotide for injection had a significant inhibitory effect on the proliferation of THP-1.The parallel line(2·2)assay method as the biopotency detection method for inhibitory effect of ossotide for injection on the proliferation of THP-1,had a good repeatability. All the samples passed the reliable test (regression P<0.01,deviation from parallel P>0.05). A20160102 batch of ossotide for injection potency was 94.50 U·mL-1,with confidence limit rate(FL)=6.14%,and the confidence limit range was from 88.87 U·mL-1 to 100.47 U·mL-1;A01160401batch of ossotide for injection potency was 92.04 U·mL-1 with FL=4.36%,and the confidence limit ranged from 88.12 to 96.15 U·mL-1. A01160406 batch of ossotide for injection potency was 83.25 U·mL-1 with FL=8.52%, and the confidence limit ranged from 76.46 to 90.65 U·mL-1. The combined analysis of the experimental results of three batches of ossotide for injection showed that the potency of ossotide for injection for inhibiting THP-1 was 90.37 U·mL-1 with FL=8.63%,and the confidence limit ranged from 82.91 to 98.51 U·mL-1. Different operators had no significant difference in the potency result of ossotide for injection,with a good repeatability. Conclusion: The biopotency detection method established in our study can be used as one of the quality control methods for the bioassay of ossotide for injection.关键词：ossotide for injection;monocyte-macrophages;bioassay tests;quality control6|6|0更新时间：2024-01-04
- 摘要：Objective: The herbal quality of Panax notoginseng may be related to the content of inorganic elements in planting soil. Method: To investigate the effect of inorganic elements in soil on the herbal quality of P.notoginseng and provide theory evidence for P.notoginseng plantation,we collected samples of P. notoginseng roots and rhizosphere soils from 16 sampling sites in Wenshan,Honghe,Yuxi,Qujing (Yunnan province). Result: The content of inorganic elements in samples of P. notoginseng roots and rhizosphere soils were determined by inductively coupled plasma mass spectrometry(ICP-MS)and the content of saponins in P. notoginseng roots were determined by high performance liquid chromatography (HPLC). The content of essential trace elements for human health in cultivated soils and medicinal herbals in Wenshan were higher than those in other 3 habitats regions, and the content of Cd in P. notoginseng cultivated soils and medicinal herbs in 4 habitats were all high. Most of the essential trace elements for human health (e.g. Al,Fe,Se) in medicinal herbals were correlated significantly positively with Mg and K in cultivated soils,and negatively with As and Hg in cultivated soils. There was no significant difference in the content of saponins in P. notoginseng roots among 4 habitats. The content of saponins in P. notoginseng roots in Wenshan and Qujing was the highest,in Honghe was the second and in Yuxi was the lowest. The content of saponins in P. notoginseng was related to the inorganic elements in medicinal herbs,but had no significant correlation with the inorganic elements in cultivated soils. Conclusion: The inorganic elements in cultivated soils influenced the herbals quality by affecting the content of inorganic elements in medicinal herbs. It was recommended to plant P. notoginseng in the soil with high content of Mg and K.关键词：Panax notoginseng;herbal quality;planting soil;inorganic elements;correlation analysis;inductively coupled plasma mass spectrometry(ICP-MS)6|6|16更新时间：2024-01-04
- 摘要：Objective: To compare the contents of inorganic elements of the above ground parts of Nardostachs jatamansi from different origins, analyze the distribution characteristics of inorganic elements, and provide experimental basis for evaluating the scientificity and rationality of the whole plant of N. jatamansi on market. Method: The contents of inorganic elements in the above ground parts of N. jatamansi from different origins were simultaneously determined by wet digestion-ICP-OES. The results were then analyzed by correlation analysis, principal component analysis,cluster analysis, partial least squares discriminant analysis and orthogonal partial least squares discriminant analysis. Result: There was a significant correlation among the elements. The multidimensional statistical analysis showed that the characteristic elements of the above ground parts of N. jatamansi were Pb,Se,As,Si,Mo,Co,Ni,Cd,Cr,Tl,Na and Sn, and this method could well cluster the samples above the ground. At the altitude of 3 400-3 500 m,the content of the most inorganic elements reached the peak value (elements beneficial for the plant growth). Conclusion: Based on the inorganic elements of the above ground parts of N. jatamansi,the altitude of 3 400-3 500 m was the optimum growth altitude,and soil as a key factor. The results provided theoretical support for rational resource distribution and whether the root and rhizome,the whole plant and the above ground parts of N. jatamansi could be replaced by each other.关键词：Nardostachs jatamansi;inorganic elements;correlation analysis;principal component analysis;cluster analysis;the above ground parts of N. jatamansi6|6|5更新时间：2024-01-04
- 摘要：Objective: To find out the serum metabolic markers of Youguiwan (YGP) in the treatment of kidney-Yang deficiency syndrome (KYDS),and to further explore the regulatory role of medicinal herbs (the Yang-tonifying herbs,the Yin-tonifying herbs and the blood-tonifying herbs) with different properties in YGP on the metabolic markers. Method: We used proton hydrogen nuclear magnetic resonance spectroscopy (1H-NMR) to study on the serum metabonomics of KYDS rats treated by YGP,and combined with pattern recognition to analyze the changes of serum metabolites,thereby finding the metabolic markers of YGP in the treatment of KYDS. At the same time,a mathematical model was established by support vector regression (SVR) to analyze and predict the influence of dose changes of medicinal herbs with different properties in YGP on the metabolic markers.Further exploration of which metabolites were regulated by medicinal herbs with different properties in YGP,and the mechanism of action of YGP was preliminary investigated. Result: Twenty-two metabolic markers were identified,including amino acid metabolism (leucine,valine,arginine,alanine,methionine,glutamate,glutamine,threonine,tyrosine and taurine),glucose metabolism (lactate,acetate and β-glucose),energy metabolism (glycerol,creatine and myo-inositol),gut metabolism[betaine/trimetlylamine oxide (TMAO)],lipid metabolism[very-low density lipoprotein (VLDL),low-density lipoprotein (LDL) and high-density lipoprotein (HDL)] and glycoprotein[N-acetyl glucoside (NAG)].Furthermore,using SVR analysis and prediction,we found that the Yang-tonifying herbs mainly acted on HDL and betaine/TMAO;the Yin-tonifying herbs primarily acted on LDL/VLDL,NAG and leucine;while the blood-tonifying herbs chiefly played a role in creatine,glutamate,lactic acid,methionine,myo-inositol and glycerol. Conclusion: The serum metabonomics based on 1H-NMR can find metabolic markers of KYDS,and the mechanism of action of KYDS regulated by medicinal herbs with different properties in YGP can be further revealed by integrating with SVR.关键词：serum metabonomics;kidney-Yang deficiency syndrome;Youguiwan;proton hydrogen nuclear magnetic resonance spectroscopy;support vector regression machine;metabolic marker group;glycoprotein8|6|2更新时间：2024-01-04
- 摘要：Objective: To study on the pharmacokinetics of chlorin e6(Ce6) phosphate-buffered saline(PBS) solution and its liposomes in tumor-bearing mice,and to verify the liver and tumor targeting of Ce6 liposomes. Method: After Ce6 solution or its liposomes were injected into tail vein of tumor-bearing mice,at different time points,the drug concentrations in serum and tissues of tumor-bearing mice were determined by HPLC,and the pharmacokinetic parameters were calculated by DAS 2.1.1 software,while the relative uptake rate(RE) within 8 h was adopted to calculate the targeting performance of Ce6 on the main organs. Result: The pharmacokinetic models of Ce6 solution and its liposomes in plasma of tumor-bearing mice were consistent with the two-compartment model,their half time of distribution phase(t1/2α) were 1.516 h and 0.507 h;their half time of elimination phase(t1/2β) were 0.457 h and 1.366 h;clearance rates(CLs) were 0.178, 0.067 L·h-1·kg-1; their AUC0-∞ were 16.734,51.475 mg·h·L-1,respectively.In liver,spleen and tumor tissues,the RE values were 1.149,1.477 and 1.277,respectively.The statistical analysis showed that there was a significant difference in major pharmacokinetic parameters between Ce6 solution and Ce6 liposomes(P<0.05). Conclusion: Compared with Ce6 solution,Ce6 liposomes has a more slowly metabolic clearance in vivo,and it distributes more rapidly and widely,it also can selectively assemble in liver and tumor tissue,liposomal formulations of Ce6 may be further developed for the treatment of liver cancer.关键词：chlorin e6;liposomes;pharmacokinetics;targetability;tissue distribution;tumor-bearing mice;relative uptake rate6|8|1更新时间：2024-01-04
- 摘要：Objective: To study the effect and mechanism of Xiaoai Jiedu decoction combined with cisplatin in inhibiting tumor growth of 4T1 tumor bearing mice. Method: BALB/c mice were subcutaneously inoculated with 4T1 breast cancer cells to establish the tumor bearing model. They were treated with cisplatin (CDDP, 2 mg·kg-1) and Xiaoai Jiedu decoction (XJF, 1×104 mg·kg-1) respectively, and the combination therapy. Body weight and tumor volume were quantified and recorded during drug treatment. The mice were put to death after 15 days, and tumor masses were stripped and weighed. The inhibition rate was calculated. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of heart, liver, spleen, kidney and tumor. The apoptosis of tumor cells was observed by TdT-mediated-dUTP nick end labeling (TUNEL) staining, and the apoptosis rate was counted. Western blot was used to detect the expressions of B-cell lymphoma-2(Bcl-2), Bcl-2 related X protein(Bax), lysine-specific demethylase 1(LSD1), Histone H3, Histone H3K4me2 and Histone H3K9me2 in tumor tissues. Result: Compared with model group, the body weight of mice in the cisplatin group decreased, and the mice in the combined group had a higher body weight than those in cisplatin group (P<0.01). The combination therapy can further reduce the volume of transplanted tumor, improve the tumor inhibition rate, and promote the apoptosis rate of tumor cells significantly compared with cisplatin therapy (P<0.05, P<0.01). The expression of Bcl-2 in tumor tissues was up-regulated, and Bax was up-regulated (P<0.05, P<0.01). Each drug group could down regulate the protein expression of LSD1, and increase the methylation of histone H3K4 and H3K9, and the combination group showed the most significant effect on the protein expression (P<0.05, P<0.01). Conclusion: Xiaoai Jiedu prescription combined with cisplatin can obviously inhibit the growth of 4T1 tumor in tumor bearing mice. Its mechanism may be related to the down-regulation of histone demethylation enzyme level.关键词：Xiaoai Jiedu decoction;4T1 tumor bearing mice;cisplatin;histone demethylation enzyme6|6|1更新时间：2024-01-04
- 摘要：Objective: To study the active mechanism of Yiyi Fuzi Baijiang San in treating ulcerative colitis in antioxidant stress signal pathway. Method: Sixty male C57BL/6J mice were randomly divided into six groups, normal group, model group, Mesalazine group, and low, medium and high-dose Yiyi Fuzi Baijiang San groups. Except for the normal group, the other groups of mice were included in the acute ulcerative colitis model. At the 3rd day of modeling, low, medium and high-dose Yiyi Fuzi Baijiang San groups were given liquid Chinese medicine with doses of 5.24, 10.48, 20.96 g·kg-1. The Mesalazine drug group was treated with 0.82 g·kg-1 of mesalazine solution. Normal group and model group were intragastrically given the same volume of double distilled water. A course of treatment was 7 days. Weight changes, stool traits and fecal occult blood of the mice were observed, and the disease activity index (DAI) score curve was drawn for each group of mice. The mice were put to death on the 10th day of the experiment; colon tissues were taken for hematoxylin-eosin(HE) staining and HI scoring; the contents of total superoxide dismutase (T-SOD) and malondialdehyde (MDA) in intestinal tissues were measured; and the expression of Nrf2 and hemeoxygenase (HO)-1 protein in colon tissues were detected by immunohistochemical method. The changes in Nrf2 mRNA expression in tissues were detected by RT-PCR. Result: Compared with the model group, the DAI score of the drug-treated mice decreased significantly (P<0.05), the histological structure of the intestinal mucosa was survived, and the HI score decreased significantly (P<0.05). The content of T-SOD in the model group was significantly lower (P<0.05), and significantly higher in the drug groups (P<0.05); the content of MDA in the model group was significantly higher (P<0.05) and decreased in the drug group (P<0.05). Immunohistochemistry showed that the expression of Nrf2 was significantly increased in the drug-treated groups compared with the model group (P<0.05). Compared with the model group, the expression of HO-1 in the low-dose group was not significantly different. High-dose group was significantly higher (P<0.05). RT-PCR detection of Nrf2 mRNA expression found that compared with the model group, high-dose group showed a high expression (P<0.05). Conclusion: Yiyi Fuzi Baijiang San can up-regulate the expression of Nrf2.Its downstream antioxidant protein HO-1 can increase the Nrf2 mRNA expression and have a therapeutic effect on ulcerative colitis.关键词：Yiyi Fuzi Baijiang San;ulcerative colitis;oxidative stress;Nrf2;hemeoxygenase-1(HO-1)6|6|11更新时间：2024-01-04
- 摘要：Objective: To investigate the efficacy and mechanism of Youguiyin, a representative decoction of tonifying kidney yang, in the rats with bleomycin induced pulmonary fibrosis. Method: A total of 25 SPF male rats were divided into five groups by the method of random number table, namely normal group, model group, as well as Youguiyin high, medium and low dose groups, with 5 rats in each group. Pulmonary fibrosis models were established by intratracheal injections of bleomycin(5 mg·kg-1), and the rats were treated with high, medium and low doses (20, 10, 5 g·kg-1) of Youguiyin for 14 days from day 15 after modeling. Then the inspiratory resistance (Ri), expiratory resistance (Re), lung compliance (Cdyn), forced vital capacity (FVC), 0.4 second rate (FEV0.4/FVC%), peak expiratory flow rate (PEF), and maximum mid expiratory flow velocity (MMF) were tested by animal lung function instrument, and FVC/lung weight were calculated; the weights of organs were recorded and the organ coefficient was calculated. The pH values of abdominal aorta (pH), oxygen partial pressure (PO2), carbon dioxide pressure (PCO2), oxygen saturation (SO2) were detected by blood gas analyzer. Renin, prostaglandin E2 (PGE2) and matrix metalloproteinase-1 (MMP-1) levels in serum were measured with enzyme linked immunosorbent assay (ELISA). Histopathological abnormalities in left lungs were evaluated by Masson's trichrome stain. Result: As compared with the normal group, intratracheal injection of 5 mg·kg-1 bleomycin can significantly increase the lung weight and lung coefficient (P< 0.05), decrease Cdyn, FVC and FVC/lung weight (P< 0.05), increase Ri (P< 0.05), increase FEV0.4/FVC% (P< 0.05), increase PCO2 in abdominal aortic blood (P< 0.05), decrease PO2 and SO2 significantly (P< 0.05), and increase PGE2 levels in serum (P< 0.05) of SD rats. Pathological examination of lung tissues showed large area of consolidation in lung tissue of model rats, and those lung tissues with consolidation were mostly formed by the non-fibrosis area with abnormal cell proliferation and fibrosis area with a large number of extracellular matrix deposition. As compared with the model group, Youguiyin medium dose group decreased the lung weight significantly (P<0.05), increased Cydn, FVC and FVC/lung (P<0.05), decreased Ri and FEV0.4/FVC% (P<0.05), decreased PGE2 levels (P< 0.05); while PO2 and SO2 levels in arterial blood were significantly increased in high dose group (P<0.05). Pathological examination showed Youguiyin can inhibit chronic inflammatory infiltration from pulmonary consolidation to normally aerated lung parenchyma. Conclusion: Youguiyin could improve the pulmonary function of model rats, which may be associated with lowing PGE2 in serum, inhibiting the diffusion of chronic inflammatory infiltration, and delaying pulmonary fibrosis.关键词：pulmonary fibrosis;bleomycin;Youguiyin;respiratory function tests;prostaglandin E2;blood gas analysis7|6|3更新时间：2024-01-04
- 摘要：Objective: To study the protective mechanism of Chaijie Yiganxian (CJYGX) on the liver fibrosis through hepatic stellate cells (HSC-T6) and transforming growth factor-β1/signal transduction protein (TGF-β1/Smad) signaling pathway. Method: Different concentrations of colchicine (1.0 mg·L-1) and CJYGX (200, 100, 50, 25, 12.5, 6.25 mg·L-1) were added to the culture medium, and the proliferation of HSC-T6 was detected by cell counting kit-8 (CCK-8) after 24 hours. The appropriate drug concentration that can inhibit the cell proliferation was screened out according to the CCK-8 results. The mRNA expressions of TGF-β1, Smad2, Smad3, Smad4 and Smad7 in HSC-T6 cells treated with CJYGX (200, 100, 50 mg·L-1) were detected by Real-time PCR, and the protein expressions of TGF-β1 and α-SMA also were detected by Western blot. Result: Compared with the blank group, CJYGX (200, 100, 50 mg·L-1) had a significantly inhibitory effect on HSC-T6 cells (P<0.05).CJYGX (200, 100, 50 mg·L-1) can significantly down-regulate the mRNA expressions of TGF-β1, Smad2 and Smad3 (P<0.05). Meanwhile, CJYGX (200, 100 mg·L-1) can significantly down-regulate Smad4 mRNA expression and up-regulated Smad7 mRNA expression (P<0.05).CJYGX (200, 100 mg·L-1) can significantly down-regulate the protein expressions of TGF-β1 and α-SMA in HSC-T6 cells(P<0.05). Conclusion: CJYGX can inhibit HSC-T6 cell proliferation, and its mechanism is related to TGF-β1/Smad signaling pathway.关键词：Chaijie Yiganxian;HSC-T6 cell;transforming growth factor-β1 (TGF-β1)/Smad pathway;proliferation;activation8|5|4更新时间：2024-01-04
- 摘要：Objective: To observe the effect of Jinlan granule on acute pharyngitis induced by Coxsackie virus, adenovirus, and influenza virus infection in infancy rabbits and infancy mice. Method: Totally 48 infancy rabbits were randomly divided into normal group, model group, Ribavirin group (22.5 mg·kg-1·d-1), Xiaoer Qingyan group (2.7 g·kg-1·d-1), Jinlan granule double dose group, equal dose group, 1/2 dose group, and 1/3 dose group (2.18, 1.09, 0.55, 0.36 g·kg-1·d-1), n=6 in each group. Acute pharyngitis model in infancy rabbits was induced by Coxsackie B4 virus infection, and then Jinlan granule was successively administered daily for 3 days beginning from the day of infection. After that, pharynx tissue was taken for pathological examination. Infancy mice were infected by adenovirus successively for 3 times to induce acute pharyngitis model in infancy mice, and then Jinlan granule was successively administered daily for 3 days beginning from the day of infection. After that, pharyngeal mucosa was taken to observe pathological changes. After infancy mice were infected by influenza virus, the number of mice death in the 2 weeks after infection was observed. Result: As compared with normal group, pharyngeal tissues of rabbits showed obvious pathological changes after infection with coxsackie virus; as compared with the model group, Jinlan granule at 4 different doses could significantly relieve the pathological changes of pharyngeal tissues. As compared with the normal group, pharyngeal tissues of mice showed obvious pathological changes after infection with adenovirus, and were significantly relieved by Jinlan granule at doses of 6.48, 3.24, 1.62, 1.08 g·kg-1·d-1. Conclusion: Jinlan granule has significant inhibitory effect on acute pharyngitis induced by coxsackie virus and adenovirus in infancy rabbits and mice respectively.关键词：Jinlan granule;coxsackie virus;adenovirus;influenza virus;acute pharyngitis8|6|2更新时间：2024-01-04
- 摘要：Objective: To investigate the effect and mechanism of modified Baitouweng Tang combined with bifidobacterium capsule in the treatment of ulcerative colitis. Method: Eight of a total of 60 SD rats were randomly selected as normal group(group A). We replicated the ulcerative colitis model with the remaining 52 rats by TNBS/ethanol enema. Rats were dissected at random at the 3rd days, so as to observe if the model was successfully established. The remaining 50 rats were randomly divided into 5 groups:modern group(group B), bifidobiogen (bifidobacterium capsule)group(group C), and high,medium and low-concentration bifidobiogen+modified Baitouweng Tang of group(group D, E, F), with 10 rats in each group. After 2 weeks of treatment, disease activity index (DAI) in rats was evaluated. HE staining was performed after the materials were collected. ELISA was used to detect serum D-lactate (D-LA), two amine oxidase (DAO) levels and colon tissue cytokine IL-8 and IL-13 levels. Real-time PCR method was used to detect the mRNA expressions of Bifidobacterium and IL-1β. Result: The mortality of group D was lower than that in group C. The weight of each drug group was higher than that of group B (P<0.05); group D had a lower mortality than group C, and the weight was higher than that of group C (P<0.05); DAI in group D was lower than that of group C (P<0.05). HE scale in D group was lower than that of group C (P<0.05); compared with group C, group D had lower D-LA, DAO and IL-8 levels and a higher IL-13 level (P<0.05).Bifidobacterium mRNA relative content in group D was higher than that of group C (P<0.05); the expression of IL-1β in group D was lower than that of group C (P<0.05). Conclusion: High concentration of modified Baitouweng Tang combined with bifidobacterium capsule may play a synergistic role in promoting colonic mucosal repair, strengthening bifidobacterium colonization in intestinal tract, reducing D-LA, DAO and IL-8, increasing IL-13 level, and down-regulating the mRNA expression of IL-1β in ulcerative colitis rats.关键词：modified Baitouweng Tang;bifidobacteria;ulcerative colitis;interleukin-8(IL-8);IL-13;D-lactute;two amine oxidase(DAO);IL-1β8|6|8更新时间：2024-01-04
- 摘要：Objective: To study the molecular mechanism on anti-tumor effect of hepatocellular carcinoma prescription (Quanfang) and its different disassembled prescriptions. Method: SMMC7721 human hepatoma cells were cultured in vitro and treated with 2.5-100 g·L-1 Quanfang, Qingrefang, Huoxuefang and Jianpifang for 24 h. Then cells proliferation was measured by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. After SMMC7721 cells were treated with 1.25-20 g·L-1 Quanfang, 5-20 g·L-1 Qingrefang and Huoxuefang and 20-120 g·L-1 Jianpifang for 24 h, related gene expression levels were detected by Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR). After SMMC7721 cells were treated with 20 g·L-1 Quanfang and Qingrefang, 10 g·L-1 Huoxuefang and 120 g·L-1 Jianpifang for 24 h, related protein expression levels were detected by Western blot and cell cycle changes were detected by PI/RNase staining solution reagent staining. Result: As compared with blank control group, Quanfang and different disassembled prescriptions inhibited the proliferation of SMMC7721 cells and showed dose-effect relationship (P<0.05). As compared with the blank control group, CDKN1B gene expression was inhibited significantly by 5-20 g·L-1 Qingrefang and 20-120 g·L-1 Jianpifang (P<0.05) in a dose-effect manner. SGK1 gene expression was inhibited significantly by 5-20 g·L-1 Quanfang and Qingrefang (P<0.05); Quanfang and different disassembled prescriptions can inhibited SGK1 phosphorylated protein expression. SGK1 gene expression was inhibited by 5-20 g·L-1 Qingre disassembled prescriptions and 20-120 g·L-1 Jianpi disassembled prescriptions (P<0.05). FOXO3 gene expression was promoted significantly respectively by 1.25, 20 g·L-1 Quanfang, 15, 20 g·L-1 Qingre disassembled prescriptions and 20 g·L-1 Huoxue disassembled prescriptions (P<0.05). As compared with blank control group, the number of G2/M cells was increased in Quanfang group (P<0.05) and was increased more significantly in Qingre group and Jianpi group (P<0.05). CyclinE1 protein expression was inhibited by Quanfang and different disassembled prescriptions, and the effect was most significant in Jianpi group. Conclusion: Quanfang and different disassembled prescriptions play a role in inhibiting liver cancer by altering cell proliferation and cycle.关键词：liver cancer;SMMC7721 hepatoma cells;therapeutic principle;cell cycles;cell proliferation8|6|2更新时间：2024-01-04
- 摘要：Objective: To investigate the effects of erigerontis herba and paeoniae radix rubra formula (Xinshao formula) on the metabolic activity of main subtypes (CYP1a2, CYP2d22, CYP2e1 and CYP3a11) of cytochrome P450s (CYP450) in mice liver and explore its mechanism. Method: Mice were randomly divided into normal group, phenobarbital group (70 mg·kg-1), and low-, middle-, high-dose Xinshao formula groups (0.31, 0.62, 1.25 g crude drug·kg-1). After administration, the livers of mice were used to prepare microsomes to determine the metabolic activities of CYP1a2, CYP2d22, CYP2e1 and CYP3a11.The mRNA and protein expression levels of these four CYP450 enzymes and pregnane X receptor (PXR) in liver tissue of mice were evaluated by quantitative real-time polymerase chain reaction (Real-time PCR) and Western blot. The effect of Xinshao formula on the activation of PXR was estimated by reporter gene assay. Result: As compared with the normal group, Xinshao formula could induce the enzyme activity of CYP3a11, up-regulate the mRNA and protein expression levels of CYP3a11 and PXR, and activate PXR (P<0.05,P<0.01). As compared with the model group, middle and high dose Xinshao formula could inhibit CYP1a2 activity (P<0.05), but had no significant effect on the mRNA and protein expression levels of CYP1a2; while Xinshao formula had no significant effects on the activities and the expressions of CYP2e1 and CYP2d22. Conclusion: Xinshao formula could induce enzyme activity of CYP3a11, and the mechanism may be associated with up-regulating PXR expression, activating PXR and promoting CYP3a11 expression. Xinshao formula also could decrease the CYP1a2 activity, but it was not related to regulation of CYP1a2 expression, and the mechanism needs a further study.关键词：Xinshao formula;cytochrome P450;pregnane X receptor(PXR);mechanism7|6|1更新时间：2024-01-04
- 摘要：Objective: To investigate the protective effect and mechanism of magnesium isoglycyrrhizinate(MgIG) on liver injury induced by paclitaxel. Method: Normal human hepatocytes LO2 were used as the research object and were divided into blank group and MgIG groups (2.5, 5, 10,20 mg·L-1). Methylthiazoletetrazolium(MTT) assay was used to detect the effect of different concentrations of paclitaxel on injuries of LO2 cells and effect of MgIG on LO2 cell viability. Then the effect of MgIG on LO2 cell injuries induced by paclitaxel was detected, with aspartate aminotransferase(AST), alanine aminotransferase(ALT) and other indexes to detect the degree of LO2 injuries by enzyme-linked immuno sorbent assay(ELISA). The effect of MgIG on cell cycle and apoptosis of LO2 cells was detected by flow cytometer. Western blot was used to detect the protein expression of apoptosis related protein. Result: As compared with blank group, 1 mg·L-1 paclitaxel had obvious inhibitory effect on the proliferation of LO2 cells(P<0.01); 5, 10, 20 mg·L-1 MgIG had certain effect on promoting the proliferation of LO2 cells(P<0.05,P<0.01), but had no significant effect on the cell morphology; 5, 10, 20 mg·L-1 MgIG can reduce the damage of paclitaxel on LO2 cells(P<0.05, P<0.01);5, 10, 20 mg·L-1 MgIG can reduce apoptosis related protein and G2/M arrest in LO2 cells induced by paclitaxel(P<0.05,P<0.01). Conclusion: MgIG can effectively reverse the LO2 damage induced by paclitaxel in vitro, and the mechanism may be related to the intervention on cell cycle and the depression on paclitaxel-induced apoptosis of liver cells.关键词：magnesium isoglycyrrhizinate injection(MgIG);paclitaxel;protection of liver injury;cell cycle;apoptosis7|6|1更新时间：2024-01-04
- 摘要：Objective: To explore the protective effects and mechanism of Shuangshen Tongmai granule (STG) in patients with ischemic reperfusion. Method: SD rats were divided into Sham operation (Sham) group,myocardial ischemia reperfusion injury (MIRI) group,diltiazem hydrochloride (DH) group and STG low dose,middle doseand high dose groups (STG-L, STG-M and STG-H).Intragastric administration was given for two weeks, and then the left anterior descending coronary artery was ligatured for modeling. The left ventricular function and Na+-K+-ATP enzymatic activity were measured;the contents of interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) in plasma were measured by enzyme-linked immunosorbent assay (ELISA). The nuclear transcription factor (NF)-κB,IκBα and Iκκβ protein levels were measured by the techniques of Western blot.Myocardial structure was observed by light microscopy. Result: As compared to MIRI group,the left ventricular function and Na+-K+-ATP enzymatic activitywere significantly improved in STG groups and DH treatment group(P<0.05), the serum levels of IL-1β, IL-6 and TNF-α were lower (P<0.05).The results of Western blot showed that the protein expressions of NF-κB were lower (P<0.05), and the protein expressions of IκBα and Iκκβ were higher (P<0.05). Conclusion: STG could alleviatemyocaridial ischemia reperfusion injury inrats by decreasing NF-κB expression protein levels and improving IκBα and Iκκβ expression protein levels.关键词：myocardial ischemia reperfusion injury (MIRI);Shuangshen Tongmai granule (STG);nuclear transcription factor kappa B (NF-κB);inhibitor kappa B kinase α (IκBα);Iκκβ7|5|3更新时间：2024-01-04
- 摘要：Objective: To understand the druggability of dichroa alkali salt (DAS) as an antimalarial drug by investigating its antimalarial efficacy and toxicity characteristics. Method: The animal models of malaria were established by inoculating strains of the parasite in an antimalarial experiment in vivo. The model mice received drugs by intragastric administration, once daily, for 4 d. Blood from the caudal vein was dynamically collected for blood smear. Giemsa staining assay was used to observe the negative conversion and revival of parasite under a microscope in each group. Finally, negative conversion ratio, anti-revival ratio, and 50% effective dose (ED50) were calculated. ICR mice were used to study the acute toxicity of DAS, observe their general state, diarrhea and death. 50% diarrhea dose (DD50) and 50% lethal dose (LD50) were calculated, while hematoxylin-eosin (HE) staining was used to observe the histopathological changes of the main organs. Result: The antimalarial experimental results showed that DAS was effective in antimalarial treatment at a lower dose, with an ED50 of 0.6 mg·kg-1 and a 95% confidence interval of 0.5 to 0.7 mg·kg-1, but no obvious effect was observed in anti-revival. The acute toxicity experiment of mice showed that after DAS was given, the mice activities were decreased, the behavior was sluggish, and the hair color was dull; the mice were in fear of cold, with poor food and water intake. Diarrhea was found in the mice with mild toxic degree, and hematochezia and death were even found in those with severe toxic degree; DD50 was 7.9 mg·kg-1, with 95% confidence interval of 6.3-10.0 mg·kg-1; LD50 was 10.8 mg·kg-1, with 95% confidence interval of 8.5-13.8 mg·kg-1. The general anatomy results showed that the stomach of mice in treatment group was obviously extended, with atrophy in spleen and cecum, and swill like changes in intestinal contents. HE staining showed that the liver, spleen, stomach and cecum had different degrees of histopathological injury. With DD50 and LD50 as the indicators, the therapeutic index (TI) of DAS was calculated as 13.17 and 18.00 respectively. Conclusion: DAS is effective but toxic for malaria at an even lower dose, with narrow safe dose window.关键词：dichroa alkali salt;antimalarial action;acute toxicity;therapeutic index6|6|3更新时间：2024-01-04
- 摘要：Objective: To investigate the effects of Astragali Radix polysaccharides on osteoblast proliferation, 1α hydroxylase (CYP27B) and 24-hydroxylase (CYP24A) gene and protein expression and preliminarily explore its mechanism in the treatment of osteoporosis (OP). Method: The MC-3T3-E1 osteoblasts were divided into 5 groups:normal group, vitamin D group (1×10-5 mmol·L-1), Astragali Radix polysaccharide high dose (10 mg·L-1) group, Astragali Radix polysaccharide middle dose (1 mg·L-1) group and Astragali Radix polysaccharide low dose (0.1 mg·L-1) group. The effect of different concentrations of Astragali Radix polysaccharides on the proliferation of MC-3T3-E1 osteoblasts at different time points (24,36,48 h) was detected by methylthiazoletetrazolium colorimetry method (MTT). The mRNA expression of CYP24A and CYP27B in MC-3T3-E1 osteoblasts was detected by real-time quantitative fluorescence polymerase chain reaction (Real-time PCR) and the protein expression of CYP24A and CYP27B in asthmatic rat MC-3T3-E1 osteoblasts was detected by Western blot. Result: MTT assay showed that the proliferation rate of MC-3T3-E1 osteoblasts could be enhanced by Astragali Radix polysaccharide at the concentrations of 0.1, 1, 10 mg·L-1,and the effect was best at 48 h. The results of Real-time PCR and Western blot showed that the mRNA and protein expression levels of CYP27B in MC-3T3-E1 osteoblasts were significantly promoted in vitamin D group as compared with the normal group (P <0.05). As compared with vitamin D group, the Astragali Radix polysaccharides (10, 1, 0.1 mg·L-1) significantly inhibited the mRNA and protein expression of CYP24A in MC-3T3-E1 osteoblast (P<0.05). Conclusion: The mechanism of Astragali Radix polysaccharide to treat osteoporosis is related to the improvement of osteoblast activity and the regulation of CYP24A, CYP27B mRNA and protein expression levels in MC-3T3-E1 osteoblast.关键词：Astragali Radix polysaccharide;Osteoblast;24-hydroxylase(CYP24A);1α-hydroxylase(CYP27B)6|7|13更新时间：2024-01-04
- 摘要：Objective: To observe the effect of modefied Si Junzitang on the expression of extracellular signal-regulated kinase1/2(ERK1/2), protein kinase B(Akt) and B cell lymphoma/lewkmia-2 assaciated X protein(Bax) in rats with cerebral ischemia/reperfusion injury, and to explore its possible protective mechanism on nerve cells. Method: Totally 55 male SD rats were randomly divided into sham operation group, model group, edaravone group, modefied Si Junzitang low-dose group, middle-dose group, and high-dose group (3.69, 7.38, 14.76 g·kg-1). The middle cerebral artery occlusion (MCAO) model was induced by suture method, after 7d treatment, the rats were sacrificed and ischemic side brain tissues were taken. Phosphorylation of ERK1/2 (p-ERK1/2), p-Akt and p-Bax protein contents were detected by Western blot; the mRNA expression levels of ERK1/2,Akt and Bax were detected by real time fluorescence quantitative polymerase chain reaction(Real-time PCR). Result: As compared with sham operation group, the p-ERK1/2,p-Akt and p-Bax protein and mRNA expression levels were significantly reduced in model group(P<0.01). As compared with the model group, the protein and mRNA expression levels of p-ERK1/2 and p-Akt were up-regulated, the expression of protein and mRNA of Bax were significantly reduced in low-dosed group, middle-dosed group, high-dosed group, with statistically significant difference (P<0.01). Conclusion: Modefied Si Junzitang can protects brain with cerebral ischemia/reperfusion injury by up-regulating the expression of p-ERK1/2 and p-Akt. reducing the expression of Bax.关键词：cerebral ischemia/reperfusion;modefied Si Junzitang;extracellular signal-regulated kinase1/2(ERK1/2);protein kinase B(Akt);B cell lymphoma/lewkmia-2 assaciated X protein(Bax)7|5|4更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of Huanglian Jiedutang on the activation of nuclear transcription factor κB (NF-κB) and the level of inflammatory factors including interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) of HT22 cells induced by β-amyloid protein 25-35 (Aβ25-35). Method: The HT22 cells were cultured and divided into five groups:blank group, model group, donepezil-treated group (0.000 9 g·kg-1) and low and high doses Huanglian Jiedutang-treated groups(2.7,8.1 g·kg-1). The HT22 cells in blank group and model group were treated with blank serum and various treatment groups were treated with corresponding drug-serum and cultured for 1 h, following the incubation with 40 μmol·L-1 Aβ25-35 for 24 h in model group and treatment groups. Then the protein expression level of p-NF-κB p65 was detected by Western blot. Nuclear translocation of NF-κB p65 was determined by immunofluorescence assay. The protein expression and mRNA expression levels of IL-1β, IL-6 and TNF-α were respectively detected by enzyme-linked immunosorbent assay (ELISA) and real time fluorescence quantification polymerase chain reaction (Reat-time PCR). Result: As compared with the blank group, the protein and mRNA expression levels of IL-1 β, IL-6 and TNF-α and the activation level of NF-κB were increased in the model group (P<0.05). As compared with the model group, low and high dose Huanglian Jiedutang and donepezil could decrease the inflammatory factors and mRNA expression levels (P<0.05) and inhibit NF-κB activation. Conclusion: Huanglian Jiedutang may reduce the Aβ25-35-induced HT22 cells inflammatory response and relieve Aβ25-35 neurotoxicity, which may be related to inhibiting the activation of NF-κB.关键词：Alzheimer's disease;β-amyloid;Huanglian Jiedutang;nuclear transcription factor-κB(NF-κB);inflammatory factor6|5|4更新时间：2024-01-04
- 摘要：Objective: To predict the active components from Saussureae Involucratae Herba by system pharmacology, and verify the prediction results. Method: Components in Saussureae Involucratae Herba were collected through the traditional Chinese medicine systems pharmacology (TCMSP) database; then active components were screened out if the druglikeness (DL) was at least 0.18.The relation graph of compounds-targets, targets-diseases and cancers-targets were constructed, so as to find the mechanisms for treating diseases with Saussureae Involucratae Herba. Next, the inhibitory effect against A549 cells was tested by SRB method. Meanwhile, the amount of prostaglandin E2 (PGE2) in A549 cells was quantified by enzyme-linked immuno sorbent assay (ELISA) kit after the treatments with the drugs at different concentrations. Result: The 24 components were screened out, and their inhibitory rates on A549 cells increased with the rise of concentration. The highest inhibitory rate was 86.22% at the concentration of about 110 mg·L-1. Compared with non-drug treated group, the amount of cellular PGE2 was decreased to different degrees (P<0.05) after the drug treatment. Conclusion: The treatment methods of Saussureae Involucratae Herba were predicted by system pharmacology, which was demonstrated to be reliable by the experiment. Therefore, this study provides a new research method for the discovery of Saussureae Involucratae Herba pharmaceutical effects.关键词：system pharmacology;Saussureae Involucratae Herba;lung cancer;prostaglandin E26|6|0更新时间：2024-01-04
- 摘要：Objective: To explore the clinical efficacy and safety of Da Huoluowan in treatment of early-to-middle stage osteonecrosis of femoral head (ONFH) with phlegm stasis syndrome. Method: A total of 180 (317 hips) eligible patients were randomly divided into the traditional Chinese medicine (TCM) group, western medicine group and TCM combined with western medicine group, 60 cases in each group. The TCM group was treated with Da Huoluowan, western medicine group was treated with diclofenac sodium sustained-release tablets, and Da Huoluowan combined with diclofenac sodium sustained-release tablets was adopted in the TCM combined with western medicine group. The treatment course was 6 months, and the patients were followed up for 6 months.The harris hip joint function scale (Harris), imaging index scale and TCM syndrome scores were applied to estimate the efficacy in the three groups before and after treatment.The changes of serum interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), superoxide dismutase (SOD) and C-reactive protein (CRP) were detected, and then the effective rate, recurrence in 6 months of follow-up and safety indexes were compared among groups. Result: The 3 cases (5 hips) withdrew from TCM group, 5 cases (8 hips) withdrew from western medicine group, and 4 cases (7 hips) withdrew from the TCM combined with western medicine group. The total effective rate in TCM combined with western medicine group was higher than the TCM group and the western medicine group (P<0.05). After treatment, the improvement in Harris scores, serum IL-1β, TNF-α, SOD and CRP levels in the TCM combined with western medicine group was significantly superior to that in TCM group and western medicine group (P<0.05), but there was no significant difference between TCM group and western medicine group. The recurrence rate in follow-up of effective patients was as follows:western medicine group < TCM group < TCM combined with western medicine group (P<0.05), and the safety was as follows:western medicine group < TCM combined with western medicine group < TCM group. Conclusion: There was no significant difference in clinical efficacy between Da Huoluowan and diclofenac sodium sustained-release tablets in treatment of ONFH with phlegm stasis syndrome, indicating the effectiveness of Da Huoluowan. In addition, it had a higher safety than diclofenac sodium sustained-release tablets. Da Huoluowan combined with diclofenac sodium sustained-release tablets showed synergistic Effect and could reduce the incidence of adverse reactions of diclofenac sodium sustained-release tablets.关键词：Da Huoluowan;osteonecrosis of femoral head;phlegm stasis syndrome;interleukin-1β (IL-1β);tumor necrosis factor-α (TNF-α);superoxide dismutase (SOD);C-reactive protein (CRP)6|6|2更新时间：2024-01-04
- 摘要：Objective: To study the clinical efficacy of Jianpi Yangxue Qufeng decoction in treating patients with blood deficiency and wind dryness type atopic dermatitis (AD) and its effect on skin barrier function. Method: Totally 132 cases of patients with blood deficiency and wind dryness type ad treated in our hospital from March 2016 to May 2017 were selected and divided into two groups according to group sequential design, with 66 cases in clinical group and 66 in control group. In addition to the external use of albolene, the control group was given Loratadine Tablets, while the clinical group was given Jianpi Yangxue Qufeng decoction. The course of treatment was 4 weeks. The treatment effect, Th1/Th2 cytokines and immunoglobulin E (IgE), skin barrier function, scoring atopic dermatitis index (SCORAD) and pruritus visual analogue scale (VAS) were compared. Result: Compared with 84.85% in control group, the total effective rate of clinical group was 95.45%. There was a significant increase (P<0.05). Compared with before treatment, interleukin (IL)-4 and IgE of the two groups after treatment decreased, while IL-2 increased (P<0.05). Compared with control group, the levels of IL-4 and IgE in patients of experimental group were lower after treatment, while IL-2 was higher (P<0.05). Compared with before treatment, transepidermal waterloss (TEWL) of the two groups after treatment decreased, while the sebum content and corneous layer water content increased (P<0.05). Compared with control group, the level of TEWL in patients of the experimental group was lower after treatment, while the sebum content and corneous layer water content were higher (P<0.05). Compared with before treatment, SCORAD scores and pruritus VAS scores in the two groups after treatment decreased (P<0.05). Compared with control group, SCORAD and pruritus VAS scores in patients of experimental group were lower after treatment (P<0.05). Conclusion: Jianpi Yangxue Qufeng decoction has a better effect on blood deficiency and wind dryness type AD, and can significantly improve Th1/Th2 cytokines and skin barrier function and reduce skin lesion and itching.关键词：Jianpi Yangxue Qufeng decoction;atopic dermatitis;clinical efficacy;skin barrier function12|6|3更新时间：2024-01-04
- 摘要：Objective: To investigate the efficacy and mechanism of modified Da Dingfengzhu therapy combined with western medicine in the treatment of patients with Yin deficiency in liver and kidney type Parkinson's disease (PD) with pain. Method: The 96 patients with Yin deficiency in liver and kidney type PD with pain were randomly divided into control group and experimental group, 48 cases in each group. All patients were given with dopasil hydrazine tablets treatment. On the basis of the treatment, the control group also received compound sea cucumber capsule, while the experimental group received modified Da Dingfengzhu therapy for 4 weeks. The clinical efficacy, motor symptoms, quality of life, emotional status, mental status and cognitive status, the plasma homocysteine (Hcy) and oxidative stress related indicators, motor nerve conduction velocity (MCV), latency (LP) and amplitude (Amp) of the common peroneal nerve, the levels of recombinant human Parkinson's disease protein 7 (PARK7) and neurotrophic factor-3 (NT-3) before and after treatment were compared between two groups. Result: The total effective rate of the experimental group was significantly higher than that of control group (P<0.05). After treatment, the motor symptoms in experimental group were significantly better than those before treatment and control group (P<0.05). The visual analogue scale (VAS) score, 39-item PD Questionnaire (PDQ-39) and unified PD rating scale (UPDRS) scores in the experimental group were significantly lower than those before treatment and control group (P<0.05). The levels of Hcy and oxidative stress in experimental group were significantly lower than those before treatment and those in control group (P<0.05). The levels of antioxidant, Amp and MCV in the experimental group were significantly higher while the LP level was significantly lower than those in control group and those before treatment (P<0.05). After treatment, the level of serum PARK7 in the experimental group was significantly lower and the level of NT-3 was significantly higher than that before treatment and that in control group (P<0.05). Conclusion: Modified Da Dingfengzhu therapy combined with western medicine can significantly improve the pain, repair nerve function, and improve the quality of life for patients in the treatment of PD with pain, and its mechanism may be related with the inhibition of excessive oxidative stress in patients.关键词：modified Da Dingfengzhu;Parkinson's disease;pain;curative effect;oxidative stress;nerve conduction9|6|3更新时间：2024-01-04
- 摘要：Objective: To observe the efficacy of modified Dingzhenwan combined with acupuncture method of consciousness restoration and resuscitation in treating patients with Parkinson's disease (PD), and investigate its possible mechanism. Method: Eighty-two patients with PD were chosen and randomly divided into control group (41 cases) and treatment group (41 cases) referring to random number table. Both groups orally took levodopa/benserazide dispersible tablet, 0.125 g/time, tid. The patients in control group were additionally treated with acupuncture method of consciousness restoration and resuscitation, 1 time/day and 6 times/week. On basis of the treatment in control group, the patients in treatment group were additionally treated with Dingzhenwan, 1 dose/day and bid. Both groups were treated for 8 weeks. Then the scores of dysfunction of tremor syndrome, symptoms of liver and kidney Yin deficiency syndrome, the Unified PD Rating Scale (UPDRS), non-motor symptoms questionnaire for Parkinson's disease (NMSQuest), Parkinson's non-motor symptom rating scale (NMSS) and clinical efficacy were compared between two groups. Serum levels of B cell lymphoma/leukemia 2 (Bcl-2), Bcl-associated X protein (Bax), cytochrome C (CytC), Caspase-9, Caspase-3 and Caspase-6 were detected and compared between these two groups. Result: After treatment, the scores of dysfunction of tremor syndrome, symptoms of liver and kidney deficiency syndrome, UPDRS-Ⅰ, UPDRS-Ⅱand UPDRS-Ⅲ in treatment group were significantly lower than those in control group (P<0.01). As compared to the control group, scores of NMSQuest and NMSS were obviously decreased in treatment group after treatment (P<0.01). Total effective rate was 87.8% in treatment group, higher than 65.85% in control group (P<0.05). After treatment, serum level of Bcl-2 in treatment group was significantly higher, while Bax, Cytc, Caspase-9, Caspase-3 and Caspase-6 levels were significantly lower than those in control group (P<0.01). Conclusion: Based on routine western medicine, Dingzhenwan combined with acupuncture method of consciousness restoration and resuscitation has significant efficacy in treating PD, and the mechanism may be associated with regulating Bcl-2, Bax and CytC levels and inhibiting Caspase-9, Caspase-3 and Caspase-6 levels.关键词：Dingzhenwan;acupuncture method of consciousness restoration and resuscitation;Parkinson's disease;liver and kidney Yin deficiency syndrome6|6|3更新时间：2024-01-04
- 摘要：Objective: To observe the clinical efficacy of modified Buyang Huanwutang combined with scalp acupuncture on unilateral spatial neglect (USN) and its effect on hemorheology. Method: One hundred and twelve patients with USN were divided into control group (56 cases) and observation group (56 cases) according to the order of admission by random number table. Both groups' patients got rehabilitation training and repetitive transcranial magnetic stimulation therapy. Patients in control group got scalp acupuncture, 1 time/day, 6 times/week. In addition to the therapy for control group, patients in observation group were also given modified Buyang Huanwutang, 1 dose/day. The treatment lasted for 8 weeks. The severity of USN was scored by linear scoring test, line deletion test and clock drawing test. Fugl-Meyer scale was used to detect the motor function. Barthel index was used for measuring the ability of daily living. Mini-mental state examination (MMSE) was used for detecting the cognitive function. And Hamilton depression scale (HAMD) and world health organization survival quality assessment scale (WHO-QOL-100) scale were used for measuring depression symptom. Before and after treatment, hemodynamic indexes were detected. Result: After treatment, the scores of linear scoring test, line deletion test and clock drawing task in observation group were lower than those in control group (P<0.01). The scores of upper and lower limbs and the total score of Fugl-Meyer scale in observation group were higher than those in control group (P<0.01). The scores of Barthel and MMSE in observation group were higher than those in control group, but the score of HAMD was lower than that in control group (P<0.01). The cognitive function and activities of daily living in observation group were improved more significantly than those in control group (P<0.05). Five composite scores in WHO-QOL-100 scale, namely physical performance, mental function, independence, social relations, and quality of life, were higher than those in control group (P<0.01). And indicators of hemorheology, such as whole blood viscosity (high and low shearing), whole blood reduced viscosity, plasma viscosity, fibrinogen, platelet aggregation rate, were all superior to those in control group (P<0.01). Conclusion: Modified Buyang Huanwutang combined with scalp acupuncture can relieve illness degree, improve the cognitive function and hemorheological indicators, reduce depression symptom, improve exercise, self-help ability and quality of life, and thus is worth clinical application.关键词：unilateral spatial neglect;stroke;Buyang Huawutang;scalp acupuncture;hemorheology9|7|4更新时间：2024-01-04
- 摘要：Objective: To analysis the pathogenesis and the medication regularity of consciousness diseases in prescription book Sheng Ji Zong Lu·Zhufengmen. Method: With Sheng Ji Zong Lu·Zhufengmen as the theoretical foundation, the pathogenic factors and pathogenesis of consciousness diseases were analyzed; the medication regularity in prescriptions for consciousness diseases was summarized through the software of traditional Chinese medicine (TCM) inheritance assistant system. Result: The top three drug categories by use frequency are deficiency-nourishing drugs, spirit-quieting drugs and drugs for relieving exterior syndrome. Top 11 high-frequency drugs were Ginseng Radix et Rhizoma, Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle, Poria,Saposhnikoviae Radix, Polygalae Radix, Cinnabaris,Cinnamomi Ramulus, Bovis Calculus, Fushen, Moschus, Borneolum Syntheticum. Commonly combined herbs included Ginseng Radix et Rhizoma combined with Fushen, Poria, Polygalae Radix, Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle; Cinnabaris combined with Moschus, Borneolum Syntheticum, Bovis Calculus. Conclusion: Deficiency of five internal organs and exogenous pathogenic wind are the core of consciousness disease pathogenesis. Ginseng Radix et Rhizoma could be used as the preferred drug for the treatment of tranquilizing and sedating the mind. Ginseng Radix et Rhizoma combined with Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle, Poria, Polygalae Radix can tonify the five organs, tranquilize the mind and promote the intelligence. Cinnamomi Ramulus combined with Zingiberis can tonify and smooth liver Qi. Saposhnikoviae Radix can dispel wind pathogens and smooth liver Qi. Cinnabaris combined with Moschus, Borneolum Syntheticum and Bovis Calculus can induce resuscitation and stay awake.关键词：Sheng Ji Zong Lu · Zhufengmen;consciousness disease;pathogenesis;medication regularity7|6|3更新时间：2024-01-04
- 摘要：In recent years,targeted drug delivery system based on drug receptors and transporters has been extensively studied.Targeted drug delivery system could enhance the concentration and efficacy of drugs in the lesion site,also could minimize the side effects of drugs.The specific membrane lectin receptors of a number of cells,glucose transporter I located at the brain microvascular endothelial cells and cancer cells could be specifically recognized by its corresponding glycosyl ligands.Drug delivery system which was glycosylated modified with mannose,galactose or glucose ligands has gained targeting properties.These glycosyl ligands exhibiting non-toxicity,biodegradability and biocompatibility,have been widely used in the glycosylated modification of drug delivery system.The targeting mechanisms,synthesis methods and their targeted ability of glycosylated modified drug delivery systems are summarized,and also a brief prospect is given in this review.关键词：glycosylation;mannose;galactose;glucose;targeted drug delivery system;transporter7|6|1更新时间：2024-01-04
- 摘要：By reviewing the papersonpheretima in recent years, the latest research progresses on the identification of cultivars, quality control of medicine, chemical constituents, pharmacological effects and artificial breeding were summarized in this paper.According to the 2015 edition of Chinese Pharmacopoeia records,pheretima includes Pheretima aspergillum, P. vulgaris, P.guillelmi and P.pectinifera.The present study showed that mitochondrial COⅠ and 16S rDNA gene of pheretima were used as DNA bar code to identify the pheretimacultivars. X-ray differaction Fourier patternand 12S rRNA gene in mitochondria were used as molecular markers to identifycultivars.The identification methods needed further research. A variety of methods have been established for controlling the quality of medicine, including the HPLC fingerprint of pheretima, the characteristic map of high performance capillary electrophoresis, the enzyme activity as an index to evaluate the quality of medicine and the quality evaluation ofmedicine by the gray relational degree model.The main chemical composition of pheretimainclude protein, amino acids, enzymes, lipids, trace elements, nucleotides and others. Its main pharmacological effects includeantiasthma, antihypertensive,antipyretic, anti-inflammatory, anticoagulant, antithrombotic, anti-tumor, enhancing immunity and so on.The main active ingredients of pheretima include protein, enzyme, amino acid, trace elements and so on.In some pharmacological actions, the active ingredients andmechanism of actionare stillunclear, so further research is needed.The artificial breeding of pheretima has rarely been reported; its breeding technology has not yet been refined,and the standardized artificial breeding technology is still in its exploration stage.The research onpheretima is still insufficient,with broad prospects for research and development. This paper may provide a reference for the further research on pheretima.关键词：Pheretima;pharmacological effects;quality control of medicine;research advances10|8|33更新时间：2024-01-04
- 摘要：Rhei Radix et Rhizoma is one of the most common traditional Chinese medicines. It has rich resources in China and was earliest recorded in Shennong's Herbal Classic. Among Rhei Radix et Rhizoma,only Rheum palmatum,R. tanguticum and R. officinale are used as medical materials and are commonly used in clinical application. It is recorded to possess the efficacy of eliminating heat and purging internal heat,cooling blood and detoxifying, dissipating blood stasis and promoting blood circulation, as well as removing dampness and relieving jaundice. The anthraquinone components in Rhei Radix et Rhizoma are 9,10-anthraquinone and the hydroxyls are distributed on two-sided benzene rings. Anthraquinones can be divided into anthraquinone and bianthraquinone according to the structure of the mother nucleus. There are about 50 reported anthraquinone components,consisting of 34 anthraquinones and 16 bianthraquinones. Anthraquinones,as an important component in Rhei Radix et Rhizoma, have broad effects of anti-inflammatory,anti-tumor,protecting cardiovascular system,liver and lung,improving brain injury and treating renal fibrosis. By referring to relevant papers at home and abroad,the researches on chemical and pharmacological effects of anthraquinones from Rhei Radix et Rhizoma in the last decade were summarized and the prospects of research and development were also analyzed for further study and comprehensive utilization of these anthraquinones. In the future, the further researches on anthraquinones and their derivatives should be done to lay a foundation for the rational application and development of new drugs with good clinical curative effect.关键词：Rhei Radix et Rhizoma;anti-inflammatory;anti-tumor;cardiovascular disease11|8|48更新时间：2024-01-04
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