最新刊期
卷 24 ， 期 16 ， 2018
- 摘要：Objective: To analyze the dynamic change rule of main index components in Scutellariae Radix during its stir-fried process with wine,explore the correlation between change of chemical composition and its pharmacological activity,and optimize frying time of Scutellariae Radix processed with wine. Method: HPLC was employed to determine the contents of four chemical ingredients(baicalin,baicalein,wogonoside and wogonin) during the stir-fried process of Scutellariae Radix processed with wine,Lipopolysaccharide(LPS) was used to induce peritoneal macrophages in mice as the model of inflammation,the change of anti-inflammatory activity during stir-fried process with wine was determined,combined with alcohol-soluble extract content and water content,cluster analysis and linear regression were used to analyze the correlation between content of index components and pharmacological activity. Result: Baicalin was the main index ingredient in Scutellariae Radix,during its stir-fried process with wine,the contents of baicalin and alcohol-soluble extract,anti-inflammatory activity all showed the trend of firstly rising and then decreasing.When frying of 18 min,the content of baicalin reached a peak value of 14.33%,and the content of alcohol-soluble extract reached the highest point of 63.00%,and the highest inhibition ratio of anti-inflammatory activity reached 92.96%. Conclusion: Stir-fried time has significant effect on the content of baicalin and pharmacological activity of Scutellariae Radix processed with wine during the stir-fried process,the best time for stir-frying is 18 min.关键词：Scutellariae Radix;correlation analysis;stir-fried with wine;baicalin;pharmacological activity;anti-inflammatory7|6|5更新时间：2024-01-04
- 摘要：Objective: To study on the microstructural changes of Ostreae Concha in the calcining process,and the effect of temperature and time on calcined Ostreae Concha were investigated. Method: Raw products of Ostreae Concha was analyzed by thermogravimetric/differential scanning calorimetry(TG/DSC).Ostreae Concha was placed in a porcelain crucible,under the conditions of different temperatures,the samples were calcined by the oven,and the Ostreae Concha was removed at every different time.After grinding,200 mesh sieves were used.The surface of calcined Ostreae Concha was analyzed by scanning electron microscope(SEM).The pore structure characteristics of calcined Ostreae Concha were analyzed by BET (Brunauer-Emmett-Teller) nitrogen adsorption.The content of CaCO3 in samples was determined by inductively coupled plasma optical emission spectrometer(ICP-OES). Result: Calcining at about 500℃ led to a significant increase in the content of quicklime.After being calcined under 300℃ for 3-4 h,the pore canal were formed on the surface of Ostreae Concha.The specific surface area formed by calcining under 300℃ was larger.The content of CaCO3 in Ostreae Concha was higher by calcining between 300-350℃. Conclusion: When calcining Ostreae Concha,it should be suitable at about 300℃,and the calcination time should be 3-4 h.Under these conditions,it will help to form pore structure,ensure large specific surface area,increase the content of CaCO3,and facilitate the development of decoction and efficacy.关键词：Ostreae Concha;calcining;microstructure;CaCO3;quicklime;raw products;thermogravimetric analysis7|6|3更新时间：2024-01-04
- 摘要：Objective: To establish the method of quantitative determination for phenolic characteristic components in Taraxaci Herba,and establish the correlation analysis of quality characterization of its phenolic characteristic spectrum.Evaluation mode of correlation analysis was used to evaluate the quality of Taraxaci Herba pieces effectively and accurately. Method: HPLC was used to determine the contents of characteristic components in 15 batches of Taraxaci Herba pieces,including protocatechuic acid,monoacyl tartaric acid,chlorogenic acid,caffeic acid,p-coumaric acid,isochlorogenic acid A,cichoric acid,galuteolin and luteolin.The phenolic characteristic spectrum of Taraxaci Herba pieces was established.The quality of 15 batches of Taraxaci Herba pieces were characterized based on the number and chemical type of peaks in characteristic spectrum.The quantity of 15 batches of Taraxaci Herba pieces was represented based on the content and peaks area of characteristic components[protocatechuic acid,monoacyl tartaric acid,chlorogenic acid,caffeic acid,p-coumaric acid,isochlorogenic acid A,cichoric acid,galuteolin,luteolin,phenolic acids(characterized by caffeic acid) and flavonoids(characterized by luteolin)]in characteristic spectrum.Correlation analysis of quality and quantity of Taraxaci Herba pieces was then performed based on reference pieces. Result: The characteristic components of Taraxaci Herba pieces had a good linear relationship and the methodological investigation were in accordance with the quantitative requirements.As the reference substance,characteristic spectrum of batch 1 contained 14 characteristic peaks(10 peaks of phenolic acids and 4 peaks of flavonoids),which all appeared on the chromatograms of 15 batches of Taraxaci Herba pieces.Taking the evaluation mode of quality characterization and correlation analysis,S3,S14,S4,S6,S9,S12 pieces contained higher effective characteristic components,while S10,S6,S12,S7,S2 pieces had higher relevance with the reference piece.Consolidated quality characterization and correlation analysis,the quality of S6,S12,S3,S10,S1 were excellent. Conclusion: The established HPLC for determining the contents of characteristic components of Taraxaci Herba is simple and accurate.The mode for the quality characterization and correlation analysis of phenolic characteristic spectrum can be used to analyze the quality and application effectiveness of Taraxaci Herba pieces,and it can effectively and accurately evaluate the quality of this pieces.关键词：Taraxaci Herba;phenols;characteristic spectrum;quality evaluation;correlation analysis;caffeic acid;flavonoids7|6|6更新时间：2024-01-04
- 摘要：Objective: To investigate the chemical constituents of Euphorbia pekinensis,and evaluate the toxicity of the obtained compounds by using zebrafish embryo. Method: The roots of Euphorbia pekinensis were extracted with 95% ethanol and then extracted with ethyl acetate to obtain the ethyl acetate fraction. The ethyl acetate fraction was then separated and purified by silica gel column chromatography, medium pressure column chromatography and preparative liquid chromatography methods,while their structures were identified by physical and chemical properties and spectral data combined with literature. At the same time,the model organism zebrafish embryo was used to evaluate the acute toxicity of each compound in E. pekinensis. The zebrafish embryo normally developed for 24 h was selected to. determine the 96 h-LC100 and 96 h-LC0 of each compound in preliminary experiment. Then in the formal experiment,eight concentrations and a blank control group were set for each compound. Development and death of zebrafish embryos at 96 h after administration were observed. The experiment was repeated for three times to calculate 96 h median lethal concentration (96 h-LC50)of different compounds on zebrafish embryos. Result: Sixteen compounds were isolated from the ethyl acetate fraction of E. pekinensis,and identified as tetradecanoic acid(1),tirucallol(2),5α-eupha-8,24-dien-3β-(3),butyl myristate(4),octacosyl(2E)-3-(4-hydroxy-3-methoxyphenyl)acrylate(5),24-propylcholesterol(6),stigmasterol(7),(3β)-stigmast-5-en-3-ylpalmitate(8),α-spinasterol(9),pinostrobin(10),oleic acid(11),manool(12),linoleic acid(13),pekinenal(14),neomotiol(15),and(3β)-3-hydroxyanosta-8,24-diene-7,11-dione(16). Conclusion: compounds 4,8,10-13 and 16 were isolated from this plant for the first time. In the evaluation of toxicity by using zebrafish embryos, the toxic compounds were compounds 10,12,14 and 16.关键词：Euphorbia pekinensis;chemical component;toxicity screening by zebrafish embryos;pinostrobin;manool7|6|3更新时间：2024-01-04
- 摘要：Objective: To isolate and identify 5-O-(4'-O-glucosylcaffeoyl)quinic acid from Lonicerae Japonicae Caulis,and develop a high performance liquid chromatography (HPLC) method for its content determination. Method: 5-O-(4'-O-glucosylcaffeoyl)quinic acid was isolated from 50% methanol extract of Lonicerae Japonicae Caulis by chromatographic methods,and its structure was identified by nuclear magnetic resonance (NMR) spectrum and mass spectrum (MS) data. Dikma Technologies PLATISILTM ODS C18 column(4.6 mm×250 mm,5 μm)was used with a mobile phase consisting of acetonitrile(A)-0.4% acetic acid solution(B) for gradient elution (0-20 min,8%-15%A)at a flow rate of 1.0 mL·min-1. The detection wavelength was 327 nm and the column temperature was 35℃. Result: 5-O-(4'-O-glucosylcaffeoyl)quinic acid showed a good linear relationship with peak area in the range of 0.15-3.07 μg,with the linear regression equation of Y=1.521 3X-3.777 7(r=1.000 0);the test sample solution was stable within 24 h; the average recovery was 95.95% (RSD 1.5%,n=6). The content of 5-O-(4'-O-glucosylcaffeoyl)quinic acid in eight batches of samples was in the range of 1.737-7.390 mg·g-1. Conclusion: The 5-O-(4'-O-glucosylcaffeoyl)quinic acid was obtained from Lonicerae Japonicae Caulis for the first time. The complete NMR data of 5-O-(4'-O-glucosylcaffeoyl)quinic acid was presented in this paper for the first time,and the method for its content determination and content limits in Lonicerae Japonicae Caulis were established. The established method is accurate and reliable,which could be applied for the quality control of Lonicerae Japonicae Caulis.关键词：Lonicerae Japonicae Caulis;5-O-(4'-O-glucosylcaffeoyl)quinic acid;structure identification;content determination9|6|1更新时间：2024-01-04
- 摘要：Objective: To establish a GC-MS/MS method for detecting 179 pesticides in Citri Reticulatae Pericarpium, in order to find out the current situations of pesticide residues in Citri Reticulatae Pericarpium. Method: The samples were extracted with acetone for twice, cleaned up by solid-phase extraction (SPE), concentrated, filtered, added with internal standard analysis protection agent, and then determined by GC-MS/MS. Result: The method of GC-MS/MS was established for determining 179 pesticides, including forbidden, limited, high-toxic and common pesticides. The recoveries of most pesticides ranged from 60% to 120%, with RSDs less than 15%. The limits of determination were below 0.01 mg·kg-1. Conclusion: This method is sensitive and accurate,and can be used for the determination of multi-residual pesticides in Citri Reticulatae Pericarpium. The findings show that many finds of pesticides were used in planting oranges,which causes serious pesticide residues pollution. Therefore, there is a risk of using Citri Reticulatae Pericarpium with excessive pesticide residues.关键词：Citri Reticulatae Pericarpium;gas chromatography-mass spectrometry;multi-residual pesticides6|6|8更新时间：2024-01-04
- 摘要：Objective: To explore the combination of pemetrexed (MTA) and cantharidin (CTD) in vitro. Method: Human colorectal cancer HCT116 cells were treated with MTA, CTD or a combination of MTA and CTD. The cell growth, apoptosis and senescence were evaluated using a colony formation assay, flow cytometry and a senescence-combined β-galactosidase assay, respectively. The expressions of pro-cysteine aspartate-specific protpase(Caspase)-3 and cleaved poly adenosine diphosphate ribose polymerase(PARP) were measured using Western blot. The combination index (CI) was calculated using CompuSyn software to evaluate synergism. Result: In HCT116 cells, the combination therapy group (50 nmol·L-1 MTA +4 μmol·L-1 CTD) showed significant decreases in cell growth (P<0.01), apoptosis (P<0.01) and cell senescence (P<0.05) compared with those of the solitary MTA therapy group. The expression of pro-Caspase-3 was significantly decreased, and the expression of cleaved-PARP was significantly increased in the combination group (P<0.01). The CI value (CI<1) showed a synergistic effect in these two agents. Conclusion: The combination of MTA and CTD synergistically suppresses human colorectal cancer HCT116 cells in vitro and exerts an antitumor effect by inducing apoptosis and senescence. Therefore, the present study provides a new candidate strategy for the treatment of colorectal cancer.关键词：pemetrexed;cantharidin;HCT116 cell;apoptosis;senescence;synergistic effect6|6|1更新时间：2024-01-04
- 摘要：Objective: To explore the pathology and relevant mechanisms of cantharidin (CTD) in inducing acute cystitis in mice through intragastric administration under the condition of 1/2 median lethal dose (LD50). Method: Balb/c mice were divided into control group and 1-14 day cantharidin administration groups. The administration groups were intragastrically given cantharidin at 1/2 LD50 for different days, while the control group was given the same amount of 0.5% carboxymethyl cellulose sodium (CMCNa). 3 h later after the last administration, bladders of 1-14 day cantharidin administration groups were collected for pathological detection by hematoxylin-eosin (HE) staining, and the bladder protein expressions of the 1 day cantharidin administration group was detected by Western blot (WB). Result: Compared with the control group, 1,3,8-12 day cantharidin administration groups showed mucosal epithelial exfoliation, mucosal epithelial hyperplasia, edema of the lamina propria with bleeding, and mucosal inflammatory cell infiltration in bladder. Western blot results showed that on the 1st day after cantharidin administration, levels of phosphorylatedprotein kinase B (p-Akt), protein kinase B (Akt), p-nuclear transcription factor-kappa B (NF-κB)p65, p-NF-κB inhibitor α(p-IκBα)and IκBα increased significantly (P<0.01). Conclusion: Acute cystitis in mice can be induced by the 3 h administration with cantharidin. After 1-7 day administration with the drug, the bladder injury caused by CTD could be repaired, but the bladder showed an irreversible and gradually aggravated inflammatory lesion on the 8-14 day. The activation of Aktsignaling pathway and NF-κB signal pathway is related to CTD-induced acute cystitis.关键词：cantharidin;bladder;inflammation;rotein kinase B (Akt) signaling pathway;nuclear transcription factor kappa B (NF-κB) signaling pathway7|6|3更新时间：2024-01-04
- 摘要：Objective: To explore the target organ for the acute toxicity of cantharidin (CTD) by establishing a cantharidin-inducing acute failure mouse model. Method: Balb/c mice were treated with different doses of cantharidin(2,3,4 mg·kg-1), and their activities were speculated. After that, the remaining mice were treated with the acute toxic dose by gavage for 3 hours, their blood samples were collected for biochemical analyses, and tissue samples were collected from livers, intestines, kidneys, and hearts for histopathological analyses. For another group,CTD was measured after treatment with 4 mg·kg-1 cantharidin for 3 h, and animals were anaesthetized with pentobarbital sodium. Result: Compared with the control group, poisoning response of the CTD mice deteriorated with the increase of concentration. The acute toxicity dose of CTD was 4 mg·kg-1, and the average death time was (4.64±1.33) h. After the administration at the acute toxicity dose, compared with control group,ECG of the mice showed abnormally upward J point and slowdown in heart rate. Cantharidin led to significant increases in the levels of alanine aminotransferase (ALT) (P<0.05), aspartate aminotransferase (AST) (P<0.05), rea nitrogen (BUN) (P<0.05), creatine kinase (CK) (P<0.01), lactate dehydrogenase (LDH) (P<0.01), and cardiac troponin (cTn) (P<0.05). Cantharidin caused inflammatory cell infiltration and myocardial injury in heart. Conclusion: Heart is the target organ of cantharidin at the acute toxic dose. Cantharidin could induce myocardial injury and cardiac structural damage.关键词：cantharidin;acute toxicity;cardiac damage;alanine aminotransferase (ALT);aspartate aminotransferase (AST);urea nitrogen (BUN);creatine kinase (CK)8|6|4更新时间：2024-01-04
- 摘要：Objective: To explore whether norcantharidin (NCTD) could reduce camptothecin (CPT)-caused myelosuppression. Method: Balb/c mice were collected. Probit analysis was made to calculate CPT median lethal dose (LD50). With 1 mg·kg-1 dose as CPT, whole blood analyzer was used to detect white blood cell(WBC) concentration at different time points within 24 h, so as to provide a reference for the subsequent detection time point in the subsequent combined administration. Subsequently, Balb/c mice were randomly divided into six groups:control group, CPT (1 mg·kg-1) group, low-dose NCTD (10 mg·kg-1) group, high-dose NCTD (20 mg·kg-1) group, CPT (1 mg·kg-1) + low-dose NCTD (10 mg·kg-1) group and CPT (1 mg·kg-1) + high-dose NCTD (20 mg·kg-1) group. After continuous gavage for 2 weeks, eyeball sampling method and whole blood analyzer were used to measure relevant indicators. Flow cytometry was used to detect the differences of bone marrow neutrophils in each group. Histopathological sections evaluated the intestinal toxicity. Result: The CPT LD50 is 1 mg·kg-1.The number of white blood cells in mice was reduced to a minimum at 6 h after the single administration with CPT. Within 24 hours after the single treatment with CPT, the numbers of leukocytes and neutrophils in CPT and NCTD combined groups increased significantly (P<0.05), with no significant change in the number of eosinophils and basophils. After continuous gavage for 2 weeks, compared with the single treatment with CPT, the number of white blood cells in CPT and NCTD combined groups also increased significantly (P<0.05), and lymphocyte was significantly increased (P<0.01). No significant changes were observed in other cells. No significant difference in the mouse bone marrow neutrophil was detected by flow cytometry, hematoxylin-eosin (HE) staining showed no significant short-term or long-term pathological changes in intestine, the main target organ of toxicity of camptothecin. Conclusion: Norcantharidin can reduce the incidence of leucopenia caused by camptothecin, without increasing the intestinal toxicity. It provides a theoretical reference for the clinical application of camptothecin and its toxicity reduction.关键词：camptothecin;norcantharidin;myelosuppression;leukopenia;enteric toxicity8|6|0更新时间：2024-01-04
- 摘要：Objective: To study on the changes of endogenous metabolites in the urine of rats with myocardial ischemia reperfusion injury(MIRI) after administrating Roudoukou-8 San,in order to explore the pathogenesis of MIRI and the mechanism of drug pre-protection. Method: Rats were randomly divided into the sham-operated group[0.5% sodium carboxymethylcellulose(CMC-Na) solution,10 mL·kg -1],the model group(0.5% CMC-Na,10 mL·kg -1) and the Roudoukou-8 San group(5.4 mg·kg-1).The urine metabolites of these three groups were characterized by UPLC-Q-Exactive-MS technique.Multivariate statistical analysis was performed by SIMCA 14.1 software to screen potential biomarkers. Result: After orthogonal partial least squares discriminant analysis(OPLS-DA),the model group was significantly separated from the sham-operated group and the Roudoukou-8 San group,partial areas of the Roudoukou-8 San group overlapped with and the sham-operated group.Eight biomarkers related to MIRI were screened and they correlated with amino acid metabolism and fatty acid metabolism. Conclusion: Roudoukou-8 San can effectively regulate the imbalance of amino acid metabolism and fatty acid metabolism related to urine metabolism in MIRI model rats and preliminarily protect MIRI through multiple pathways.关键词：Roudoukou-8 San;myocardial ischemia reperfusion injury;metabolomics;urine;amino acid metabolism;fatty acid metabolism8|7|1更新时间：2024-01-04
- 摘要：Objective: To study on the therapeutic effect of Gastrodiae Rhizoma decoction on A53T α-synuclein transgenic Parkinson mice and its influence on the expression of apoptosis related pathways. Method: A53T α-synuclein transgenic mice with Parkinson disease were used as experimental subjects,and model group,Gastrodiae Rhizoma group,blank group and positive group(madopar group) were also set up.The pathological changes of substantia nigra neurons in mice were determined by genotype identification,hematoxylin-eosin(HE) staining and behavioural experiment to judge the success of the model establishment,the Illumina HiSeq 2500 platform sequencing technologies was used to detect differential expression of transcripts of all genes in Gastrodiae Rhizoma group.The genes related to Parkinson's protection and metabolic pathways were screened by functional annotation and enrichment analysis. Result: It could improve the substantia nigra neuron pathological changes in A53T α-synuclein transgenic Parkinson mice after treating Gastrodiae Rhizoma decoction,and transcriptome analysis showed that the mechanism mainly involved the inhibition or activation of proto oncogene of rat sarcoma(Ras),p38 mitogen activated protein kinase(p38 MAPK) and Ca2+ related apoptosis pathways.Compared with the model group,the expression of dopamine third receptor(DRD3),neuropeptide Y(NPY),Ras protein specific guanine nucleotide releasing factor 1 (RASGRP1),calcium/calmodulin-dependent 3',5'-phosphodiesterase 1A(PDE1A) in Gastrodiae Rhizoma group were up-regulated,and apoptotic genes,such as proto oncogene of c-Fos,p38 MAPK and protein kinase 3 activated by human mitogen activated protein kinase (MAPKAPK3) were down-regulated. Conclusion: Gastrodiae Rhizoma decoction has a certain therapeutic effect on A53T α-synuclein transgenic Parkinson mice,its mechanism may be mainly related to the inhibition of many apoptotic related signaling pathways.关键词：Gastrodiae Rhizoma;decoction;A53T α-synuclein;transgene;Parkinson mice;transcriptome;apoptosis6|6|4更新时间：2024-01-04
- 摘要：Objective: To predict the targets of Yimai granules by bioinformatics,according to the adjustment of target genes,to determine its signaling pathways and the molecular mechanism of Yimai granules. Method: Bioinformatics was used to predict the potential regulatory genes of all drugs in Yimai granules.According to the principles of formulating prescription,a Venn diagram was drawn for all target genes of Yimai granules that acted on atherosclerosis(AS),the overlapping genes were defined as target genes.Before and after administration,the hemogram changes of the AS model mice and the corresponding target gene regulation methods were used to determine the mechanism of Yimai granules to improve AS.The experimental group consisted of 60 ApoE-/- mice,they were randomly divided into 5 groups and fed continuously for 12 weeks.Twelve rats in model group were fed with high fat and high cholesterol diet+normal saline by intragastric administration;twelve rats in the high,medium and low dosage groups of Yimai granules were fed with high fat and cholesterol diet+high,medium and low dose of Yimai granules,respectively;twelve rats in the positive group were fed with high fat and high cholesterol diet+atorvastatin calcium tablets. Result: Yimai granules can reduce levels of triglyceride(TG),total cholesterol(TC),low density lipoprotein cholesterol(LDL-C) and high-density lipoprotein cholesterol(HDL-C) in mice,and the effect was positively correlated with the dose of Yimai granules.The lowering of TG,TC,LDL-C and HDL-C in the high-dose group of Yimai granules was weaker than those in the atorvastatin calcium tablets group.The targets of Yimai granules included carboxylesterase 1(CES1),3-hydroxy-3-methylglutaryl coenzyme A reductase(HMGCR),peroxisome proliferator-activated receptor(PPAR)D,PPARG,liver X receptor-α(LXR-α) and adenosine triphosphate(ATP)-binding cassette transporter A1(ABCA1). Conclusion: Yimai granules can activate the corresponding signaling pathway by up-regulating the expression of target genes of CES1,HMGCR,PPARD,PPARG,LXR-α and ABCA1,so as to achieve the therapeutic effect on AS.Bioinformatics can be used to predict and verify the action targets of formulas,which can provide a method to explain the mechanism of clinical formulas.关键词：bioinformatics;atherosclerosis;gene target;signaling pathway;formulas;Yimai granules;atorvastatin calcium tablets8|6|1更新时间：2024-01-04
- 摘要：Objective: To explore the effect of Wenshen Yisui Shengxue prescription on anemia in RA rats and bone morphogenetic protein(BMP)/Smad signaling pathway in liver. Method: A total of 42 male SPF grade Wister rats were randomly divided into groups twice according to the body mass stratification:normal group, sham operation group, model group, uremia(2.33 g·kg-1) group and Wenshen Yisui Shengxue(3.5 g·kg-1) group. In the model group and the treatment group, 180 mg·kg-1·d-1 adenine was given by gavage in the morning, and the treatment group was treated with the corresponding medicine in the afternoon. The normal group, the sham operation group and the model group were given the equal volume of deionized water. After 12 weeks of treatment, blood and serum were collected after the rats were anesthesia. SCr and urea nitrogen (BUN), uric acid (UA), whole blood test red cell (RBC), hemoglobin (HGB) were detected; kidney tissue and liver tissue were fixed; hematoxylin-eosin(HE) staining was used to observe kidney pathology; immunohistochemistry and in situ hybridization technique were used to detect bone morphogenetic protein 6 (BMP6), Smad4, Smad1/5/8, p-Smad1/5/8, hepcidin (hepcidin) mRNA and protein expressions in rat liver. Result: Compared with normal group and sham operation group, the body mass of the model group decreased obviously (P<0.01), the levels of serum BUN and SCr increased obviously (P<0.01), and the pathological damage of kidney was obvious. Compared with model group, the general state of the Wenshen Yisui Shengxue group was obviously improved, and the levels of blood RBC and HGB were increased (P<0.01); compared with the normal group and the sham operation group, the model group showed high expressions of BMP6, Smad4, Smad1/5/8 and hepcidin in liver (P<0.01). Compared with the model group, BMP6, Smad4, Smad1/5/8, p-Smad1/5/8 and hepcidin protein expressions in the Wenshen Yisui Shengxue group were significantly reduced (P<0.01). Conclusion: Wenshen Yisui Shengxue prescription has a certain effect in alliviating anemia in RA rats, which may be realized by intervening BMP/Smad signaling pathway in liver.关键词：kidney marrow;renal anemia;rats;bone morphogenetic protein(BMP)/Smad;hepcidin6|6|1更新时间：2024-01-04
- 摘要：Objective: To observe the effect of effective fractions of Huangqisan (HQS) on mRNA expression of sterol regulatory element binding protein-1c (SREBP-1c) and its target genes in insulin-resistant HepG2 cells. Method: The effect of HQS on the activity of HepG2 cells was detected by methye thiazolye telrazlium(MTT) method. The model of insulin-resistant HepG2 cells was induced and established by high glucose and high insulin. While inducing the model, HepG2 cells were treated with different concentrations of HQS, and metformin (MET) was used for positive control. The glucose uptake of cells was detected by fluorescent D-glucose homologue (2-NBDG), and the contents of total triglyceride(TG) and free fatty acids(FFA) in cells were also detected by assay kits. Meanwhile, intracellular lipid droplets were observed by oil red O staining. Besides,Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR) method was used to detect mRNA expressions of SREBP-1c, acetyl CoA carboxylase 1 (ACC1), fatty acid synthase (FAS) and stearoyl-CoA desaturase 1(SCD1). Result: According to the findings of MTT experiment, three different concentrations 25 mg·L-1 (HQS-L), 50 mg·L-1 (HQS-M) and 100 mg·L-1 (HQS-H) of HQS were selected for treating cells, and the treating time was 24 h. Compared with control group, the glucose uptake of cells significantly decreased in model group (P<0.01), and the contents of TG and FFA markedly increased (P<0.01). Besides,a large number of red vesicular lipid droplets were also observed in cytoplasm, and the mRNA expressions of SREBP-1c, ACC1, FAS and SCD1 all apparently up-regulated (P<0.01). Compared with model group, different concentrations of HQS all significantly increased the glucose uptake (P<0.05, P<0.01). HQS-H and HQS-M reduced the contents of TG and FFA in cells (P<0.01) and the number of intracellular lipid droplets. Meanwhile, HQS-L decreased FFA content (P<0.05). Besides, HQS-H apparently down-regulated the mRNA expressions of SREBP-1c, ACC1, FAS and SCD1 (P<0.05, P<0.01), and HQS-M also down-regulated the mRNA expressions of SREBP-1c, FAS and SCD1 (P<0.05). Although HQS-M could also inhibit ACC1 mRNA expression, there was no statistically significant difference. Conclusion: HQS may alleviate insulin resistance in HepG2 cells by inhibiting SREBP-1c mRNA expression and reducing lipid synthesis.关键词：effective fractions of Huanqisan;lipotoxicity;insulin resistance;HepG2 cells;sterol regulatory element binding protein-1c (SREBP-1c)8|6|1更新时间：2024-01-04
- 摘要：Objective: To observe the effect of Pingweisan on blood lipid, blood glucose and hepatocyte nuclear factor-1β(HNF-1β/TCF2) expression in mice with glycolipid metabolism disorder induced by high-fat diet, in order to explore the possible molecular mechanism of Pingweisan in regulating glucose and lipid metabolism in mice. Method: A total of 40 specific pathogen free (SPF) C57BL/6J adult male mice were randomly divided into normal group (n=8) and model group (n=32), which were respectively fed with normal diet and high-fat diet for 10 weeks. Since the 11th week, the model group was randomly divided into model group (n=8), Pingweisan group (n=8), metformin group (n=8) and simvastatin group (n=8). The Pingweisan group was given Pingweisan 6 000 mg·kg-1·d-1, the metformin group was given metformin 300 mg·kg-1·d-1, the simvastatin group was given simvastatin 2 mg·kg-1·d-1, and the normal group and model group were given the same volume of distilled water only; that is to say, each group was given by gavage 10 mL·kg-1·d-1 for 4 weeks. During feeding, body weight, abdominal circumference, blood lipids, blood glucose and insulin were continuously monitored. After the intervention, liver and kidney tissues were taken to detect the mRNA and protein expression of HNF-1β. Result: At the end of the 10th week, compared with the normal group, the weight, abdominal circumference, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and fasting plasma glucose (FPG) of the model group increased (P<0.05), while fasting insulin (FINS) decreased (P<0.05). After 4 weeks of treatment, compared with the normal group, the body weight, abdominal circumference, blood lipid and 4 hours of blood glucose of oral glucose tolerance test (OGTT) of the model group were significantly increased (P<0.01). Relative mRNA and protein expressions of FINS and HNF-1β were decreased (P<0.05). Compared with the model group, the weight, abdominal circumference, TC, TG, and OGTT 4 hours of blood glucose of the Pingweisan group were all decreased (P<0.05), whereas the relative mRNA and protein expressions of FINS and HNF-1β were all increased (P<0.05). The body weight, abdominal circumference, TC, TG, LDL-C, OGTT 4 hours of blood glucose of the metformin group were all decreased (P<0.05), and the relative mRNA and protein expressions of FINS and HNF-1β were all increased (P<0.05). The body weight, abdominal circumference and the blood lipid of the simvastatin group were significantly decreased (P<0.01), and the relative mRNA and protein expressions of FINS and HNF-1β were increased (P<0.05). Conclusion: After 10 weeks of high-fat diet, a mouse model of glycolipid metabolism disorder can be established. Pingweisan can regulate the blood lipid and blood glucose levels by regulating the expression of HNF-1β.关键词：Pingweisan;blood lipid;blood glucose;hepatocyte nuclear factor-1β (HNF-1β);C57BL/6J mice7|5|1更新时间：2024-01-04
- 摘要：Objective: To investigate the protective effect of rutin on SH-SY5Y cells injured by 1-methyl-4-phenyl pyridinium (MPP+). Method: SH-SY5Y cells were exposed to various doses of rutin for 4 h,and then treated with 1 mmol·L-1 MPP+ for 48 h. The cell viability was detected with methye thiazdye telrazlium (MTT) assay,and the nuclear morphology was assessed with Hoechst 33342 staining. Reactive oxygen species (ROS) was measured with fluorescence microscope,and mitochondrial membrane potential (ΔΨm) was determined with flow cytometry. The protein levels of Cytochrome C (Cyt-C) and phosphorylated extracellular regulated protein kinases 1/2 (p-ERK1/2) were detected by Western blot. Result: Compared with the blank group,the cell viability of the MPP+-treated group was reduced to 49.6%,the shrunk cell body and nuclear condensation were also observed after Hoechst 33342 staining. Meanwhile,the ROS level was significantly increased, and the membrane potential was obviously reduced. Western blot results showed that the expression of Cyt-C was increased, whereas p-ERK1/2 was decreased(P<0.01). However,pretreatment with rutin prior to MPP+ exposure reduced the cell viability,ameliorated the cell morphology,inhibited the increasement of ROS and restored the membrane potential. Moreover,pretreatment with rutin can inhibit the increase of Cyt-C and the decrease of p-ERK1/2 induced by MPP+ in SH-SY5Y cells(P<0.05,P<0.01). Conclusion: Rutin can protect the SH-SY5Y cells against the damage induced by MPP+,which may be correlated with anti-oxidant activity and ERK activation pathway.关键词：rutin;1-methyl-4-phenyl pyridinium (MPP+);SH-SY5Y cell;neuroprotective effect;oxidative stress7|5|5更新时间：2024-01-04
- 摘要：Objective: To explore the multi-target regulatory effect of paeoniflorin, synephrine, glycyrrhizin and their combination on 5-hydroxytryptamine(5-HT) signaling pathway[tryptophan hydroxylase 1(TPH1)/serotonin transporter(SERT)/5-HT3R/5-HT4R], in order to explore the synergistic interaction between the ingredients and the action mechanism of the traditional Chinese medicine(TCM) prescription Sinisan on the visceral hypersensitivity rats. Method: The SD rats were randomly divided into normal group, model group, pinaverium bromide group, paeoniflorin group, synephrine group, glycyrrhizin group, paeoniflorin+glycyrrhizin group, paeoniflorin+synephrine group, glycyrrhizin + synephrine group, paeoniflorin + glycyrrhizin + synephrine group and Sinisan group. The irritable bowel(IBS) visceral hypersensitivity rat model was established by the comprehansive stress method. The intestinal sensitivity of the rats was evaluated by the minimum capacity threshold of abdominal withdrawal reflex (AWR). The protein and mRNA expressions of 5-HT, TPH1, SERT, 5-HT3R, 5-HT4R in colon were determined by enzyme linked immunosorbent assay(ELISA), Western blot and Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR). Result: Compared with the normal group, the minimum capacity threshold value of AWR significantly decreased in the model group (P<0.05), with significant differences in protein and mRNA expressions of TPH1,5-HT3R, 5-HT4R and SERT (P<0.05, P<0.01). Compared with the model group, the levels of 5-HT, TPH1, 5-HT3R, and 5-HT4R were significantly improved, with an multi-target correction effect on TPH1, 5-HT3R, 5-HT4R mRNA expressions in the paeoniflorin + simvin + glycyrrhizin group (P<0.05, P<0.01). The value of minimum capacity threshold and the performance status significantly improved (P<0.05, P<0.01), with an interaction between the components in the group. Conclusion: The combination of paeoniflorin, simvin and glycyrrhizin has a synergistic effect on the regulation of 5-HT synthase (TPH1) and related receptors (5-HT3R, 5-HT4R) in the colon of visceral hypersensitivity rats. The multi-target rectifying effect of the active ingredient combination on the 5-HT pathway may be an important mechanism for Sinisan to alliviate the visceral hypersensitivity of rats.关键词：paeoniflorin;synephrine;Glycyrrhizin;5-hydroxytry ptamine(5-HT);Sinisan8|6|12更新时间：2024-01-04
- 摘要：Objective: To observe the effect of Yangxinkang tablet on cardiomyocyte apoptosis in rabbit with heart failure after myocardial infarction through protein kinase B (Akt)/AMP-activated kinase (AMPK)-the mammalian target of rapamycin (mTOR) pathway. Method: The rabbit model of heart failure was established through ligation of coronary artery. A total of 30 experimental animals were randomly divided into model group, Yangxinkang group, AMPK inhibitor group (10 mg·kg-1 Compound C, intraperitoneal injection, 2 times a day), Akt inhibitor group (10 mg·kg-1 casodex, intragastric administration,2 times a day) and mTOR inhibitor group (0.5 mg·kg-1 rapamycin, intragastric administration, 2 times a day), and a blank control group was also set up, with 5 in each group. Yangxinkang tablet (0.51 g·kg-1) were intragastrically administered once a day. Blank control group and experimental groups were given the equivalent volume of distilled water for 4 weeks. Heart function was detected by color Doppler ultrasound, Bcl-2 and Bax protein expressions were detected by Western blot, and cardiomyocyte apoptosis was detected by TdT-mediated DUTP nick end labeling(TUNEL). Result: Compared with the blank control group, the LVEF value of the model group decreased (P<0.01), the expressions of Bcl-2 and Bax protein increased (P<0.05, P<0.01), the Bcl-2/Bax ratio decreased (P<0.01), and cardiomyocyte apoptosis rate was increased (P<0.01). Compared with the model group, the LVEF value of the Yangxinkang group was increased (P<0.01), the expression of Bax protein was decreased (P<0.01), the Bcl-2/Bax ratio was increased (P<0.01), and the cardiomyocyte apoptosis rate was decreased (P<0.01). Compared with each inhibitor groups, the LVEF value of Yangxinkang group was increased (P<0.01), Bcl-2/Bax ratio was increased (P<0.01), and the cardiomyocyte apoptosis rate was decreased (P<0.01). Conclusion: Yangxinkang tablet can regulate the expressions of Bcl-2 and Bax protein in cardiac myocytes, reduce cardiomyocyte apoptosis through Akt/AMPK-mTOR pathway and improve the cardiac function of heart failure model after myocardial infarction.关键词：Yangxinkang tablet;heart failure;protein kinase B(Akt)/AMP-activated kinase(AMPK)-the mammalian target of rapamycin(mTOR) pathway;cardiac function;cardiomyocyte apoptosis7|6|8更新时间：2024-01-04
- 摘要：Objective: To study the protective effect of bacuchiol on hepatic stellate cells induced by transform growth factor-β(TGF-β) and its mechanism. Method: The activity of each group of cells was detected by methye thiazolye telrazlium(MTT), in order to screen out the safe and effective concentration of bacuchiol. This experiment was divided into blank group, model group (10 μg·L-1 TGF-β), bacuchiol group (1×10-5 mol·L-1), estradiol group (1×10-6 mol·L-1), and their activities were detected. Superoxide dismutase (SOD), Glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) of each group were detected by enzyme linked immunosorbent assay(ELISA); Real-time quantitative-PCR (Real-time PCR) was used to detect the changes in matrix metalloproteinase-1 (MMP-1) and collagen-Ⅰ (Col-Ⅰ) mRNA expressions in hepatic stellate cells induced by TGF-β; Western blot method was used to detect the changes of MMP-1 and Col-Ⅰ protein content in hepatic stellate cells induced by TGF-β. Result: The 1×10-5mol·L-1 bacuchiol can effectively improve the activity of SOD and GSH-Px in hepatic stellate cells, and also reduce MDA content in cells and the expressions of Col-Ⅰ and MMP-1 mRNA and protein (P<0.01). Conclusion: Bacuchiol has a protective effect on hepatic stellate cells induced by TGF-β mainly by improving the activity of antioxidant enzymes in cells and reducing the content of MMP-1, Col-Ⅰ mRNA and protein expressions in cells.关键词：bacuchioll;transform growth factor-β(TGF-β);Hepatic Stellate cells;antioxidant enzymes;matrix metalloproteinase-1 (MMP-1);collagen-Ⅰ(Col-Ⅰ)6|5|4更新时间：2024-01-04
- 摘要：Objective: To investigate the protective effect of Ploygonati Odorati Rhizoma (PORE) extract against mitochondrial damage and apoptosis in myocardial cells of myocardial ischemia-reperfusion injury in rats. Method: Sixty male Wistar rats were randomly divided into sham operation group, model group, PORE group and ginkgolide B group. Myocardial ischemia-reperfusion model was established through the reversible left anterior descending coronary artery ligation, and the rats were provided with the myocardial ischemia for 30 min and the reperfusion for 120 min. 30 min before ligation, the PORE group was given 100, 200, 300 mg·kg-1 ip PORE, and the ginkgolide B group were given 60 mg·kg-1 ip, the model group and the sham operation group were given the same volume of saline ip; Hemodynamic indexes of the rats were record. The apoptosis of ventricular myocytes in each group was observed by terminal-deoxynucleoitidyl transferose mediated nick end labeling(TUNEL) staining. The morphology and pathological changes of myocardial mitochondria were observed by transmission electron microscopy. The levels of free fatty acid (FFA), adenosine monophosphate (ATP), adenosine monophosphate (AMP), lactate (LAC) in myocardium of the rats were detected by enzyme-linked immunosorbent assay (ELISA). The protein expressions of cysteine aspartate-specific protease(Caspase)-3, 9 and 12 in ventricular myocytes were detected by Western blot. Myocardial mitochondria were isolated for the determination of free calcium content. Result: Compared with the sham operation group, the left ventricular systolic pressure and the maximal rising rate of left ventricular pressure were decreased in the model group; the left ventricular end diastolic pressure, and the maximum descending rate of left ventricular pressure were increased. Myocardial apoptosis was obvious. The contents of FFA and LAC in myocardium were increased, the expressions of Caspase-3, 9 and 12 were increased, the ratio of ATP/AMP was decreased, the mitochondrial membrane was disintegrated, and the inner crest was disappeared; compared with the model group, the hemodynamic parameters, myocardial cell apoptosis and myocardial mitochondrial pathological changes of the PORE groups and the ginkgolide B group were improved, the levels of FFA and LAC in myocardial tissue and the levels of Caspase-3, 9, 12 were decreased, and the ratio of ATP/AMP was increased (P<0.05). Conclusion: PORE can improve myocardial ischemia-reperfusion injury and reduce myocardial apoptosis, the mechanism may be related to the protection of mitochondria and the enhancement of myocardial cell mitochondrial energy metabolism.关键词：Ploygonati Odorati Rhizoma extract;reperfusion injury;apoptosis;mitochondria;energy metabolism6|6|8更新时间：2024-01-04
- 摘要：Objective: To investigate the protective effect and related mechanism of Oxalidis Coriniculatose Herba on acute hepatic injury induced by carbon tetra-chloride in rats based on Toll-like-receptor-2 (TLR-2)/nuclear factor-kappa B (NF-κB) signaling pathway. Method: A total of 48 female rats were randomly divided into normal group, model group, silymarin (0.12 g·kg-1) group, and high, middle and low-dose Oxalidis Corniculatase Herba(16, 8, 4 g·kg-1) groups, with 8 in each group. Except for the normal group and model group, all of the remaining rats were given drugs by gavage for 10 days, twice a day. Two hours later, except for the normal group, all of the remaining rats were injected with 12% carbon tetra-chloride olive oil solution (5 mL·kg-1) via abdominal cavity to establish the acute liver injury model. After 16 hours, the blood was taken from the retrobulbar arteries, and the liver tissues were collected to make htoxylin eosin(HE) pathological sections. The activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST),total-superoxide dismutase (T-SOD),glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in serum of rats were detected by biochemical method. The level of tumor necrosis factor alpha (TNF-α), interleukin-1 β(IL-1β) and interleukin-6 (IL-6) in serum were assayed by enzyme linked immuno sorbent assay (ELISA); and protein expressions of TLR-2 and NF-κB in liver tissue of rats were tested by Western blot. HE pathological sections of liver tissues of rats were observed under microscope. Result: Compared with the normal group, the serum activity of ALT, AST and the content of MDA significantly increased, whereas T-SOD and GSH-Px activity decreased (P<0.01), and the levels of IL-1β, TNF-α, IL-6 in serum and the hepatic expressions of TLR-2, NF-κB increased in the model group (P<0.01). After intervention with Oxalidis Corniculatase Herba, the activities of ALT, AST and the levels of MDA, IL-1β, IL-6, TNF-α in serum were obviously reduced (P<0.05, P<0.01), while the activities of GSH-Px and SOD in serum were improved (P<0.05, P<0.01), and the expressions of TLR-2 and NF-κB in liver tissue were inhibited (P<0.05, P<0.01). The pathological sections also showed that the liver injury degree in rats was significantly alleviated. Conclusion: Oxalidis Corniculatase Herba can ease the acute liver injury in rats induced by carbon tetra-chloride olive oil solution, the mechanism may be related with the intervention of TLR-2/NF-κB signaling pathway and the inhibition of oxidative stress and inflammatory responses.关键词：Oxalidis Corniculatase Herba;acute liver injury;oxidative stress;inflammatory response;Toll-like-receptor-2 (TLR-2)/nuclear factor-κB (NF-κB) signaling pathway6|6|5更新时间：2024-01-04
- 摘要：Objective: To investigate the Effect of quercetin on proliferation,apoptosis,adhesion,migration and invasion ability of U937 cells and expressions of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9). Method: U937 cells were cultured in vitro and treated with quercetin at various doses(0,10,20,40 μmol·L-1)for 24,48,72 h. The proliferation of U937 cells was measured by cell counting kit-8(CCK-8). U937 cells were randomly divided into blank group,quercetin 10 μmol·L-1 group,quercetin 20 μmol·L-1 group and quercetin 40 μmol·L-1 group. Apoptotic rates were evaluated by terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling(TUNEL). Adhesion of the cells was detected by the cell adhesion assay. Migration ability was evaluated by the wound healing assay. Invasion ability was evaluated by the transwell chamber assay. Expressions of MMP-2 and MMP-9 were detected by Western blot. Result: The proliferation of U937 cells was inhibited by quercetin. As compared with the blank group,quercetin 10 μmol·L-1 group,quercetin 20 μmol·L-1 group and quercetin 40 μmol·L-1 group significantly increased apoptotic indexes in U937 cells(P<0.05,P<0.01); and significantly decreased adhesion rates,migration rates,numbers of invasive cells,expressions of MMP-2 and MMP-9 in U937 cells(P<0.05,P<0.01). Conclusion: Quercetin could significantly inhibit the migration and invasion ability of U937 cells, and mechanism may be associated with down-regulating expressions of MMP-2 and MMP-9 in U937 cells.关键词：quercetin;acute myeloid leukemia;migration;invasion;matrix metalloproteinase-2(MMP-2);matrix metalloproteinase-9(MMP-9)6|6|4更新时间：2024-01-04
- 摘要：Objective: To elucidate the effect and mechanism of flavonoids from Mori Folium (FM) on improving blood glucose in diabetic rats. Method: After successful modeling by intraperitoneal injection of streptozotocin (STZ), twenty-four 8-week-old male SD rats were randomly divided into model group, Pioglitazone group, FM group with 8 rats in each group according to their levels of blood glucose and body weight, and another 8 normal SD rats with the same age were also used as a normal group. After treatment for 6 weeks, their fasting blood glucose (FBG), cholesterd (CHO), total triglyceride (TG), free fatty acids (FFA), aspartate aminotransferase (AST), fasting insulin (Fins), and homa insulin-resistance (HOMA-IR) were measured. Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) and Western blot were used to quantify the mRNA and protein expressions of certain targets in the liver. Result: As compared with the normal group, the body weight, FBG, Fins, HOMA-IR, CHO, TG, FFA were significantly increased in model group (P <0.05,P<0.01); the mRNA and protein expressions of protein kinase C (PKC) were increased significantly in model group (P<0.05,P<0.01), but the mRNA expressions of adenylate kinase2 (AK2), and peroxisome proliferator activated receptor gamma coactivator 1-alpha (PGC-1α) were decreased significantly (P<0.05,P<0.01). As compared with the model group, body weight, FBG, Fins, HOMA-IR, CHO, TG, and FFA were significantly decreased in FM group (P <0.05,P<0.01); the mRNA and protein expressions of PKC in liver tissues were decreased significantly (P<0.05,P<0.01), but the mRNA expressions of AK2, and PGC-1α were increased significantly in FM group (P<0.05,P<0.01). Conclusion: It was demonstrated that FM may play an anti-diabetic effect by inhibiting the PKC pathway induced by FFA and improving the energy homeostasis based on the expression of AK2 and PGC-1α.关键词：flavonoids from Mori Folium;type 2 diabetes;free fatty acid;protein kinase C;adenylate kinase 2;peroxisome proliferator activated receptor gamma coactivator 1-alpha8|6|11更新时间：2024-01-04
- 摘要：Objective: To investigate the effects of Zhuangtongyin(ZTY)on the mRNA expression of angiotensin Ⅱ (AngⅡ), angiotensin-receptor 1 (AT-1R) and connexin 43 (Cx43) in rats with myocardial ischemia-reperfusion injury (MIRI), and explore the protective mechanism of ZTY pre-treatment on cardiac myocytes in rats during MIRI process. Method: SPF grade SD rats were randomly divided into normal group, sham-operated group (thread without ligation), model group, ZTY low dose group (6.8 g·kg-1), ZTY middle dose group (13.6 g·kg-1), ZTY high dose group (27.2 g·kg-1) and compound Danshen group (0.08 g·kg-1). After drug treatment for continuous 4 weeks, the left anterior descending coronary artery was ligated for 30 min, and then reperfused for 60 min to establish reperfusion injury model in blood stasis syndrome type coronary heart disease. ECG monitoring was performed during operation and the occurrence of arrhythmia was recorded. After reperfusion, their heart tissues were taken and the Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) was used to detect the AngⅡ, AT-1R and Cx43 mRNA expression in myocardial tissues; Western blot method was used to detect the AT-1R and Cx43 protein expression in myocardial tissues. Result: ZTY low dose group, middle dose group and high dose group can reduce the incidence of arrhythmia in rats with myocardial ischemia reperfusion as compared with model group (P<0.05). As compared with the normal group and sham operation group, the AngⅡand AT-1R mRNA expression levels were increased significantly, and Cx43 expression levels were reduced in model group, all ZTY groups and compound Danshen group (P<0.05). As compared with the model group, the AngⅡand AT-1R mRNA levels were reduced and Cx43 mRNA expression levels were increased in all ZTY groups and compound Danshen group (P<0.05), and the effect was most significant in ZTY middle dose group (P<0.05). There was no significant difference between ZTY groups and compound Danshen group in AngⅡ, AT-1R and Cx43.There was no significant difference between normal group and sham operation group.Compared with normal group,the myocardial ischemia-reperfusion injury rats,ZTY low, middle, high dose group and compound Salvia Pellet dose group myocardial organization AT-1R is increased sinificantly(P<0.05),Cx43 reduced(P<0.05). Compared with ischemia-reperfusion injury rats, ZTY low, middle, high dose group and compound Salvia Pellet dose group myocardial organization AT-1R is reduced sinificantly(P<0.05),Cx43 increased (P<0.05),ZTY middle dose group better than other group (P<0.05).There was no sinificant difference between ZTY each dose group and compound Salvia Pellet dose group in AT-1R and Cx43. Conclusion: ZTY can reduce myocardial ischemia-reperfusion arrhythmia and have protective effects on myocardial ischemia-reperfusion injury, and the mechanism may be associated with regulating AngⅡ-(AT-1R)-Cx43 pathway and up-regulating Cx43 expression in myocardial tissues.关键词：Zhuangtongyin;blood stasis syndrome of coronary heart disease;reperfusion injury;angiotensin Ⅱ(AngⅡ);angiotensin-receptor 1(AT-1R);connexin 43 (Cx43)6|6|3更新时间：2024-01-04
- 摘要：Objective: To predict the active compounds and effective anti-tumor targets of Ziyin Huatan decoction (ZYHTD) by network pharmacology, and explore its anti-tumor mechanism through ‘multi-compounds, multi-targets, and multi-pathways’ model. Method: The chemical constituents of ZYHTD were searched through TCM chemical component database and previous literature. The potential active compounds were screened based on oral bioavailability (OB) and drug likeindex (DL) in TCMSP database. The potential targets of active compounds were obtained based on DRAR-CPI docking server, and then the anti-tumor targets in Drugbank and TTD database were collected and matched to obtain the peculiar anti-tumor targets of ZYHTD. Further, the effective constituents-targets network of ZYHTD was established by using Cytoscape software and then the network topology was studied. Finally the biological function and KEGG pathway of all anti-tumor targets were analyzed through DAVID database. Result: A total of 25 active compounds, 92 anti-tumor targets were predicted, from which PDPK1, G3P, DHB1, AURKA, PPAP, S10A9 and NQO1 were the main anti-tumor targets of ZYHTD. The functional anti-tumor mechanism of ZYHTD may associated with regulating the biological processes such as cell proliferation, cell migration, apoptotic process, phosphorylation, and signal transduction, as well as the signaling pathways such as FoxO, PI3K-Akt, ErbB, Rap1, MAPK, Ras and FcERI. Conclusion: This research preliminarily evidenced the main targets and related pathways for the anti-tumor effects of ZYHTD, laying a foundation for further experimental studies to reveal the mechanism.关键词：Ziyin Huatan decoction;tumor;network pharmacology;mechanism7|6|0更新时间：2024-01-04
- 摘要：Objective: To investigate the clinical efficacy of Ganlu Xiaodu Dan in patients with damp-heat syndrome of hepatitis B virus(HBV) associated with acute-on-chronic liver failure(HBV-ACLF)and the application value of noninvasive assessment by FibroScan. Method: A total of 108 patients with HBV-ACLF were selected and randomly divided into treatment group (54 cases) and control group (54 cases). Both groups adopted the basic medical treatment including bed rest, enteral nutrition, supplementing albumin or fresh plasma, antiviral treatment and so on. On the basis of the basic medical treatment,the treatment group additionally received Ganlu Xiaodu Dan. The major traditional Chinese medicine (TCM) syndrome symptoms (yellow skin and eyes, scanty dark urine,abdominal fullness and distention,lack of energy,loose or sticky stool and so on), liver function biochemical indexes[alanine aminotransferase(ALT),aspartate aminotransferase(AST),serum total bilirubin(TBIL),albumin(ALB),and prothrombin activityprothrombin time activity(PTA)], and liver measurement of FibroScan(Kpa), were observed in both groups before and after treatment. Result: In the aspect of TCM syndrome treatment efficacy, the total effective rate of the treatment group was significantly higher than that of the control group(P<0.05). After treatment, both groups showed significant decrease in ALT, AST, TBIL and measurement of FibroScan, and the effect was more obvious in the treatment as compared with the control group(P<0.05), but ALB had no significant difference after treatment. Conclusion: Based on the basic medical treatment, Ganlu Xiaodu Dan could not only enhance the curative effect in patients with HBV-ACLF significantly, but also improve the measurement value of the FibroScan markedly. As compared with the routine invasive tests of liver function biochemical index, Fibroscan provides a new method for evaluating the clinical efficacy of Ganlu Xiaodu Dan for treatment of HBV-ACLF, with real-time, dynamic, non-invasive and comprehensive technical characteristics, so it is worthy of clinical promotion.关键词：Ganlu Xiaodu Dan;hepatitis B virus(HBV);liver failure;damp-heat syndrome;FibroScan9|6|3更新时间：2024-01-04
- 摘要：Objective: To observe the variation of nitric oxide(NO) in serum and endothelin-1 (ET-1) in the plasma in patients with diabetic peripheral neuropathy treated by Duhuo Jisheng Tang and evaluate its clinical significance. Method: On the basis of routine blood glucose control, 128 outpatients and inpatients with diabetic peripheral neuropathy were randomly divided into traditional Chinese medicine(TCM) oral treatment group (62 cases) and TCM steaming group (66 cases). The patients in TCM oral treatment group orally took Duhuo Jisheng Tang, 1 dose/day, 400 mL, bid; the patients in TCM steaming group were treated with Duhuo Jisheng Tang steamed externally,1 dose/day, 1 L, bid. One treatment course was 10 days and the patients in both groups received 3 treatment courses. The changes NO in serum and ET-1 levels in plasma before and after treatment were observed and compared between two groups; percentage of clinical symptom scores reduction and current perception threshold (CPT) were used for quantitative detection and determination of clinical efficacy. Meanwhile, the adverse reactions were monitored. Result: Both oral administration and streaming treatment of Duhuo Jisheng Tang can significantly increase the level of NO in serum (P<0.05)and decrease the level of ET-1 in the serum of patients with diabetic peripheral neuropathy(P<0.05). The total effective rate for clinical symptom scores reduction was 83.9%and 86.4%respectively in TCM oral treatment group and TCM steaming treatment group. The CPT values of bilateral median nerves and gastrocnemius nerves were significantly reduced in both groups(P<0.05), but there was no significant difference between two groups. Conclusion: Duhuo Jisheng Tang can significantly increase the level of NO in serum and decrease the level of ET-1 in the plasma of patients with diabetic peripheral neuropathy. This may be one of the effective mechanisms for the treatment of diabetic peripheral neuropathy,providing a novel therapy for diabetic peripheral neuropathy.关键词：diabetic peripheral neuropathy;Duhuo Jisheng Tang;traditional Chinese medicine therapy;nitric oxide(NO);endothelin-1 (ET-1)6|6|3更新时间：2024-01-04
- 摘要：Objective: To discuss the effect of differentiation maintenance treatment of traditional Chinese medicine(TCM) in patients with acute exacerbation type chronic obstructive pulmonary disease (COPD), and investigate its effects on recent symptoms, pulmonary function, and long term acute exacerbation. Method: One hundred and sixty eight patients were randomly divided into control group and observation group by random number table with use of SAS software. Patients in control group received budesonide and formoterol fumarate powder for inhalation, 2 inhalations/days, 2 times/days. Based on the treatment of control group, the patients in observation group received additional differentiation maintenance treatment of TCM. patients with lung-spleen Qi deficiency syndrome orally took Jinkexi capsules, 4 capsules/time, tid. Patients with lung-kidney Qi deficiency syndrome orally took Gushen Dingchuan pills, 1.5-2.0 g/time, tid. Patients with lung and kidney Qi-Yin deficiency syndrome took Bushen Ruifei decoction, 30 mL/time, qd. Patients with heat-phlegm took Qingqi Huatan pills, 6 g/time, tid. Patients with cold-phlegm took Kechuanshun pills, 5 g/time, tid. Patients with blood stasis received Xuefu Zhuyu pills, 8 g/time, bid. The course of treatment was 6 months for both groups, and 6 months of follow-up were recorded after the treatment. Before and after treatment, scores of clinical symptoms and modified British medical research council (mMRC) were graded. Levels of 1 second forced expiratory volume (FEV1), percentage of estimated value of FEV1 (FEV1%) and severity of air flow limitation were evaluated. Levels of interleukins-6 (IL-6), IL-8, tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) were detected. And acute exacerbation was recorded and compared during the follow-up. Result: The total effective rate for COPD was 90.91% in observation group, higher than 74.6% in control group (χ2=6.058, P<0.05). After treatment, the scores of clinical symptoms and mMRC in observation group were lower than those in control group (P<0.01), and FEV1% was higher than that in control group (P<0.05). The levels of CRP, IL-6, IL-8, and TNF-α in serum were lower than those in control group (P<0.01). And the limited severity of airflow was lower than that in control group (χ2=6.354, P<0.05). During the follow-up period, mean number of acute aggravation times and number of hospitalization in observation group were less than those in control group (P<0.05). Conclusion: Differentiation maintenance treatment of TCM can relieve clinical symptoms, ameliorate pulmonary function and dyspnea, improve quality of life, control inflammatory reaction, decrease times of acute exacerbation in a long term and reduce acute aggravating risk factors, with both significant short-term and long-term benefits.关键词：chronic obstructive pulmonary disease;acute exacerbation;differentiation maintenance treatment;time of acute aggravation times8|6|3更新时间：2024-01-04
- 摘要：Objective: To observe the effect of Xuanfei Tongfu decoction on severe pneumonia combined with gastrointestinal dysfunction in middle and old age, and investigate its protection effect on gastrointestinal function and inflammatory factors. Method: One hundred and four eligible patients were randomly divided into control group (52 cases) and observation group (52 cases) by random number table. Patients in control group got anti-infection treatment, anti-inflammation treatment, symptomatic treatment and supportive treatment, and they got mosapride citrate oral solution, 10 mL/time, 3 times/days, lactobacillus and enterococcus powder, 2 g/time, 3 times/days. Based on the treatment in control group, the patients in observation group additionally got Jiawei Xuanfei Tongfu decoction, 1 dose/day. The treatment course was 14 days in both groups. Before and after treatment, CURB65, SMART-COP and clinical pulmonary infection score (CPIS), scores of traditional Chinese medicine(TCM), gastrointestinal dysfunction (GIDF), acute physiology and chronic health evaluationⅡ score (APACHEⅡ) and scores of multiple organ dysfunction syndrome (MODS) were graded, and levels of serum motilin (MTL), gastrin (GAS), D-lactic acid, diamine oxidase (DAO), procalcitonin (PCT), interleukins-6 (IL-6), IL-8 and tumor necrosis factor-α (TNF-α) were detected. In addition, the fatality rate in ICU (within 2 weeks), duration of hospitalization in ICU and time of mechanical ventilation were recorded. Result: The markedly effective rate was 59.62% in observation group, higher than 38.46% in control group (χ2=4.656 4, P<0.05). The duration of hospitalization in ICU and time of mechanical ventilation in observation group were shorter than those in control group (P<0.01). The fatality rate in ICU was 11.54% in observation group, lower than 15.38% in control group, with no statistically significant differences between two groups. The scores of CURB65, SMART-COP, CPIS, TCM, GIDF, APACHEⅡ, and MODS in observation group were lower than those in control group (P<0.01). The serum GAS, D-lactic acid and DAO levels were lower while level of MTL was higher than those in control group (P<0.01). The serum PCT, TNF-α, IL-6 and IL-8 levels in observation group were lower than those in control group (P<0.01). Conclusion: Adjuvant therapy of Jiawei Xuanfei Tongfu decoction based on routine western treatment can control clinical symptoms, regulate gastrointestinal hormones, promote repair of intestinal mucosal barrier and recovery of gastrointestinal function, inhibit inflammatory reaction, relieve degree of disease and can shorten healing time in treatment for patients with severe pneumonia combined with GIDF.关键词：severe pneumonia;gastrointestinal dysfunction;Xuanfei Tongfu decoction;gastrointestinal hormones;inflammatory factors7|6|13更新时间：2024-01-04
- 摘要：Daodi herb is an essence in traditional Chinese medicine.As an important carrier for excellent Chinese cultural heritage and an important source of innovation,the inheritance and innovation of Daodi herbs is an urgent scientific issue which needs to be expanded.With the rapid development of modern science and technology,Chinese scholars conducted pioneering exploration on theoretical and practical research of Daodi herbs.Here,with theoretical research as the breakthrough point, we summarized five basic theories:environmental ecology theory; variety continuity and origin change theory; quality ecology theory; stress-effect theory and herb genomics theory according to the academic ideas,the formation time,representative scholars and monographs.The environmental ecology theory reflected the academic inheritance based on the holistic view which named"Tian Yao Ren He Yi (union of nature, herb and human)".Variety continuity and origin change theory,quality ecology theory, stress-effect theory and herb genomics theory reflected the academic thought innovation based on inheritance.This paper mainly elucidated the innovation theory of Daodi herbs,stated the core academic views and research contents,introduced the new techniques and new methods in the modern research of Daodi herbs,and analyzed the correlation and continuity between 5 theories.The innovation theories and related research systems would provide strong support for the origin of Daodi herbs and new germplasm breeding.Besides,we mapped the evolution of "Daodi herbs" names and also discussed and prospected the new problems, new demands and research thoughts in the research of Daodi herbs.关键词：Daodi herbs;heritage;innovation;traditional Chinese medicine6|6|6更新时间：2024-01-04
- 摘要：Alismatis Rhizoma is the dry tuber of Alisma orientale,it has the effects of clearing dampness and promoting diuresis,disperse heat,turbidity-reducing and lipid-lowering;and it is one of the commonly used Chinese herbal medicines in clinical practice.At present,there are relatively few studies on Alismatis Rhizoma,mainly related to the fields of pharmacognosy,processing,chemistry,pharmacology and others,among them,there are many researches on the chemical composition,pharmacological effects,clinical application and processing,there are also many review articles in these fields.This article mainly summarizes the literatures about of harvest,processing in production place,processing and quality evaluation of Alismatis Rhizoma,analyzes the research status of the medicinal materials and its decoction pieces in the above four aspects,summarizes the existing problems,and combines the national standardization project of traditional Chinese medicine for prospecting and analysis,in order to solve the problems existing in the actual production and use process.关键词：Alismatis Rhizoma;processing in production place;quality evaluation;processing;harvesting;standardization;fingerprint11|6|6更新时间：2024-01-04
- 摘要：Depression is a common psychiatric disorder with a rising incidence year by year. In addition to the common chemical drugs, the clinical application of traditional Chinese medicines has drawn much attention for depression. As a classic prescription for dispersing stagnated liver and relieving depression, Xiaoyaosan has a good antidepressant effect in experimental and clinical studies. The papers in last ten years were reviewed and the results showed that Xiaoyaosan had good antidepressant effect in the different depression-like animal model, and the antidepressant mechanism was related to modulation of monoamine neurotransmitter levels, effect on neurotrophic factors and the related pathways, regulation of hypothalamic-pituitary-adrenal (HPA) axis function, inhibition of the inflammatory response and anti-oxidation activity, et al. The papers of the nearly five years showed that Xiaoyaosan or its derivatives alone and Xiaoyaosan combined with western medicine or with other therapies were widely used in clinical treatment of various patients with depression, with reliable curative effect and few adverse reactions. The basic research status of Xiaoyaosan's antidepressant effect from the view of disassembled prescription and the components isolated from Xiaoyaosan was summarized for the first time, and the results showed that Bupleuri Radix, Angelicae Sinensis Radix, Paeoniae Radix Alba, Atractylodis Macrocephalae Rhizoma and Menthae Haplocalycis Herba were the main effective components for its antidepressant effect.关键词：Xiaoyaosan;antidepressant;depression-like animal model;clinical application;disassembled prescription;petroleum ether fraction7|7|9更新时间：2024-01-04
- 摘要：Steroidal alkaloids are nitrogenous derivatives of natural steroids,with dual properties of steroids and alkaloids in chemical properties and biological activities. Steroidal alkaloids act as the main pharmacodynamic components of Fritillaria,Buxus sinica,Veratrum nigrum,Solanum nigrum,and other traditional Chinese medicines, and they are also found in some common foods such as potatoes,tomatoes and eggplants. Modern pharmacological studies have shown that steroidal alkaloids are highly active in anti-tumor,cardiovascular system,antibacterial,insecticidal and other activities,but also had high toxicity. The quantitative analysis of steroidal alkaloids is difficult because of its particularity of structure. In the review of quantitative analysis methods for steroidal alkaloids in the literature from 1998 to 2017, the methods for determining total content of steroid alkaloids mainly included spectrophotometry-acid dye colorimetry,Enzyme-linked immunosorbent assay (ELISA) and titration. High performance liquid chromatography (HPLC) was generally used in the quantitative analysis of specific steroid alkaloids,mainly including HPLC-UV,HPLC-ELSD,HPLC-MS and HPLC-CAD. In this paper,the related studies on the quantitative analysis of steroid alkaloids in the past 20 years were summarized,and the advantages and limitations of the various methods were analyzed,in order to improve and promote the control methods for the quality of related traditional Chinese medicines and the safety of related foods,and provide a reference on the analysis methods for the the further development and utilization of steroidal alkaloids and related traditional Chinese medicines.关键词：tetrahydroisoquinoline alkaloid;content determination;HPLC;acid dye colorimetry6|6|1更新时间：2024-01-04
- Postal code：100700
- Tel：010-84076882 Email：syfjx_2010@188.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0