最新刊期
卷 24 ， 期 18 ， 2018
- 摘要：In order to inherit the ancient academic thought,expand the scope of clinical application of Sinitang,this study collected and collated items of attending illness of Sinitang and its modified prescriptions from ancient medical books and medical record books.Meanwhile,we collected and collated items of attending illness of Sinitang and its modified prescriptions from medical cases of Fuyang school for reference.Sinitang and its modified prescriptions are used to treat limbs cold,diarrhea,vomiting,abdominal pain and sweating in ancient medical books;Sinitang and its modified prescriptions are used to treat diarrhea,vomiting,nausea,abdominal pain,cold limbs,irritability and sweating in medical record books.Sinitang may treat cholera,heart failure,shock,ulcerative colitis,acute and chronic enteritis,angina pectoris,myocardial infarction,thrombophlebitis and so on.关键词：Sinitang;illness;literature research;Fuyang school;medical record;classical formulas13|621|5更新时间：2024-01-04
- 摘要：Objective: To discuss the best processing technology of Rehmanniae Radix with Amomi Fructus. Method: Model of Kidney-Yin deficiency and wet-sleepy spleen and stomach was adopted to evaluate the effect of Rehmanniae Radix processed with Amomi Fructus on enhancing Yin(synergism) and eliminating the greasy,the best proportion of Amomi Fructus to Rehmanniae Radix was selected.The content of acteoside,monosaccharides(D-glucose,D-fructose) was used as the evaluation indexes,combined with sensory evaluation index of color black as lacquer and central airspace,single factor experiments were employed to select the technology of Rehmanniae Radix processed with Amomi Fructus. Result: The mass ratio of Amomi Fructus to Rehmanniae Radix was 2%.Optimum processing technology was as following:100 kg of Rehmanniae Radix used 30-50 kg of rice wine;added 1 kg of Amomi Fructus powder before and after steaming,respectively;mixed evenly with rice wine and Amomi Fructus powder,soaked completely,steamed about 4 hours at 110-120℃,until black inside and outside,and central blackening for degrees,dried at low temperature to about 80% drying,used Amomi Fructus powder to mix evenly. Conclusion: This optimized technology is stable and feasible,this study can provide basis for elucidating the mechanism of enhancing Yin and eliminating the greasy of Rehmanniae Radix processed with Amomi Fructus.关键词：Amomi Fructus;Rehmanniae Radix;processed with herbal juice;wet-sleepy spleen and stomach;acteoside;D-fructose;D-glucose9|366|5更新时间：2024-01-04
- 摘要：Objective: To prepare and characterize the inclusion complex of volatile oil in Artemisiae Annuae Herba and hydroxypropyl-β-cyclodextrin(HP-β-CD),and investigate in vitro antiviral activity of this inclusion complex. Method: HP-β-CD inclusion complex of volatile oil in Artemisiae Annuae Herba were prepared by saturated aqueous solution method,hand grinding method and single phase solution method.Some analytical methods were applied for characterization of the inclusion complex.The inhibitory effects of volatile oil in Artemisiae Annuae Herba and its HP-β-CD inclusion complex on respiratory syncytial virus(RSV) and cossac virus type A16(CA16) were investigated by in vitro cell culture method. Result: Single phase solution method was the best inclusion method,and its best inclusion ratio was 80.62% when ratio of HP-β-CD and volatile oil was 8:1,concentration of ethanol was 100%,inclusion temperature was 25℃ and inclusion time was 2 h.A series of evident characteristics suggested the formation of this inclusion complex.The median effective concentration(EC50) of volatile oil in Artemisiae Annuae Herba and its HP-β-CD inclusion complex to RSV were 3.12,0.28 mg·L-1,respectively;and EC50 were 9.14,0.59 mg·L-1 against CA16,respectively. Conclusion: The preparation process of HP-β-CD inclusion complex of volatile oil in Artemisiae Annuae Herba by single phase solution method is stable and feasible,and it can significantly increase inclusion rate of volatile oil in Artemisiae Annuae Herba.Antiviral activity on RSV and CA16 of the inclusion complex is stronger than before inclusion.关键词：Artemisiae Annuae Herba;volatile oil;hydroxypropyl-β-cyclodextrin;inclusion complex;respiratory syncytial virus;cossac virus type A16;single phase solution method8|538|8更新时间：2024-01-04
- 摘要：Objective: Based on the DNA barcoding technology,a method of investigating medicinal sources for poisonous herbal medicine(Bungarus Parvus) was established to investigate medicinal sources of this medicine in Chinese herbal markets and drug stores. Method: Firstly,a reference cytochrome C oxidase subunit I (CoⅠ) barcode library was established for the authentic Bungarus Parvus and its main adulterants.Then,51 samples of the commercially available Bungarus Parvus were collected from herbal markets and drug stores,and then CoⅠ sequences for the head and the tail tissue of each samples were obtained.Finally,the species identification of head and tail tissues of these commercially available samples was achieved by constructing the neighbor-joining(NJ) phylogenetic tree. Result: A total of 60 CoⅠ sequences were obtained from the authentic Bungarus Parvus and its main adulterants.The maximum intra-specific variation of Bungarus multicinctus was 0.020 1,whereas the minimum inter-specific variations of B. multicinctus and their main adulterants (B. fasciatus,Lycodon rufozonatus and Sinonatrix annularis) were 0.156 1,0.208 8 and 0.209 3,respectively;the maximum intra-specific variation of B. multicinctus was less than its minimum inter-specific variations.The authentic Bungarus Parvus and its main adulterants showed independent branches in the NJ phylogenetic tree and could be discriminated clearly.CoⅠ sequences could be successfully obtained from both the head and the tail of all the commercially available Bungarus Parvus samples and the investigation results showed that 29 of B. multicinctus,20 of L. rufozonatus and 2 of S. annularis.The total of adulterants accounted for 43% and the results based on heads and tails were the same. Conclusion: DNA barcoding technology can be used to investigate the medicinal source of the commercially available Bungarus Parvus.The market of Bungarus Parvus is in disorder and has serious quality problems with a lot of adulterants.关键词：Bungarus Parvus;cytochrome C oxidase subunit I;DNA barcoding;investigation of medicinal source;Bungarus multicinctus;adulterant8|244|2更新时间：2024-01-04
- 摘要：Objective: To explore composition of metabolites of Ophiocordyceps sinensis and Isaria cicadae by gas chromatography-mass spectrometry(GC-MS) metabonomics,in order to provide scientific basis for quality control of O. sinensis. Method: Samples were extracted by 50% methanol and then were subjected to GC-MS analysis.Metabolites were identified by NIST and GMD databases.Principal component analysis(PCA) and partial least squares-discriminant analysis(PLS-DA) were applied to explore sample classification and screen metabolites which contributed to discrimination,respectively. Result: A total of 64 metabolites were identified from all samples,mainly including amino acids and their derivatives,sugars alcohols,organic acids,fatty acids and others.PCA results showed clear separation of O. sinensis and I. cicadae,indicating that the metabolic characterization between those two samples was significantly different.Furthermore,21 metabolites responsible for discrimination were screened out.The contents of D-mannitol,trehalose,proline and pyroglutamic acid were significant higher in O. sinensis.Whereas,other compounds,including myo-inositol,γ-aminobutyric acid,ornithine and threonine showed higher values in I. cicadae. Conclusion: The chemical composition of O. sinensis and I. cicadae differs greatly,which suggesting that I. cicadae can not be used as alternative of O. sinensis.关键词：Ophiocordyceps sinensis;Isaria cicadae;gas chromatography-mass spectrometry;metabolomics;amino acids;principal component analysis;D-mannitol8|496|8更新时间：2024-01-04
- 摘要：Objective: To provide powerful tools for molecular marker-assisted breeding and resources protection by analyzing the simple sequence repeat (SSR) information in the transcriptome of Fritillariae Cirrhosae Bulbus. Method: The Illumina HiSeq2000 technology platform was used for high throughput sequencing of the bulbs and leaves of Fritillariae Cirrhosae Bulbus, and MISA software was used for SSR loci analysis of Unigene. Result: 3 817 SSR loci were found in a total of 44 973 unigenes obtained from the transcriptome, distributing in 3 367 unigenes in the examined sequences. Frequency of occurrence for SSRs was 8.49%,and the density of distribution was 1/10.37 kb on average. Tri-nucleotide and di-nucleotid repeats were predominated with an occurrence frequency of 56.51%and 27.06%,respectively. CCG/CGG of Tri-nucleotide was the most frequent one among all the repeat types, followed by AG/CT of di-nucleotid repeats. The number of SSR repeats was mainly between 4-12 times,and the sequence length of 24.55%SSR was higher than 20 bp. Conclusion: The SSR loci in the transcriptome of Fritillariae Cirrhosae Bulbus show high frequency, rich types,and high polymorphism,which will might be applied in the study on the candidate gene mining and marker-assisted breeding.关键词：Fritillariae Cirrhosae Bulbus;transcriptome;simple sequence repeat (SSR);polymorphism8|206|12更新时间：2024-01-04
- 摘要：Objective: To establish the grade evaluation standard for Buleuri Radix decoction pieces,and provide theoretical basis for building the grade standard for root and rhizome regular shape Chinese medicinal decoction pieces. Method: Compound quality constant is a comprehensive grade evaluation method for traditional Chinese medicine decoction pieces,which is based on but even better than a traditional way. This method was based on the quality constant,where the internal index and appearance character of Buleuri Radix decoction pieces were combined effectively by investigating the appearance character,volatile oil,extracts,saikosaponin a and d contents in traditional Chinese medicine decoction pieces. In this way, the evaluation method of compound quality constant was constructed to perfect and establish the grade standard of Buleuri Radix decoction pieces. Result: Twenty batches of different quality samples were collected and tested,and finally,19 qualified batches of them were chosen as researcher objects. The results revealed that the range of the compound quality constant of these samples was from 1.616 to 8.414 and Buleuri Radix decoction pieces could be divided into three grades:the compound quality constant of first grade was ≥ 7.871;the second grade was <7.871 but ≥ 5.602;while the third grade was <5.602. Conclusion: Compound quality constant can be used to scientifically,objectively and reasonably classify the decoction pieces,solve the problems existing in the relative quality constant evaluation methods,and provide a new method and a new model for the classification of multi-indicator components in traditional Chinese medicine decoction pieces, as well as a beneficial reference for further establishing and perfecting the comprehensive quality evaluation system of Buleuri Radix decoction pieces.关键词：Buleuri Radix decoction pieces;compound quality constant;extract;volatile oil;grade evaluation8|284|19更新时间：2024-01-04
- 摘要：In this study,ultra performance liquid chromatography tandem three four quadrupole time-of-flight mass spectrometry (UPLC-Triple-TOF-MS) was used to discriminate three isomers(congmunosides VI,Ⅶ and B) in Aralia elata. Through the analysis of MS2 spectra of[M+Na]+(CE=90 eV) on congmunosides VI and Ⅶ (1 and 2),both produced the same fragmentation ion peaks at m/z 981.498 2[Y0α+Na]+,819.439 1[Y0α-Glc+Na]+,527.161 4[C3β+Na]+,509.150 4[B3β+Na]+and 365.105 7[C3β-Glc+Na]+,while the MS2 spectrum of congmunoside B (3) of[M+Na]+(CE=90 eV) produced two different characteristic fragmentation ion peaks of m/z 671.198 2 and 689.208 7,corresponding to the[B4β+Na]+and[C4β+Na]+. Thus,the structure of congmunoside B(3) could be effectively distinguished with other two isomers of saponins(1 and 2) via[B4β+Na]+and[C4β+Na]+ions. Through the further analysis MS2 spectrum of[M+NH4]+and[M+H]+(CE=40 eV) for congmunosides Ⅵ and Ⅶ(1 and 2) yielded same ions at m/z 455.350 7 and 437.341 2,belonging to the[A-H2O+H]+and[A-2H2O+H]+. The aglycone of congmunoside Ⅵ(1) was echinocystic acid with the ion intensity ratio of[A+H-2H2O]+(m/z 437.34) to[A+H-H2O]+(m/z 455.35) ascribed to<1.The aglycone of congmunoside Ⅶ(2) was hederagenin with the ion intensity ratio of[A+H-2H2O]+(m/z 437.34) to[A+H-H2O]+(m/z 455.35) ascribed to >1.Based on the above fragmentations of[M+NH4]+and[M+H]+ions,congmunosides Ⅵ and Ⅶ(1 and 2) could be further distinguished. The study results demonstrated that three isomers from A. elata could be distinguished rapidly,easily and accurately by electrospray ionization tandem mass spectrometry.关键词：UPLC;mass spectrometry discrimination;isomers;triterpene saponins;congmunoside Ⅶ;congmunoside Ⅵ;congmunoside B8|218|1更新时间：2024-01-04
- 摘要：Objective: To conduct a qualitative analysis on the chemical constituents of the aqueous extract from raw Nauclea officinalis by using ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS/MS) method. Method: Chromatographic separation was performed on AcclaimTM RSLC 120 C18 column(2.1 mm×100 mm,2.2 μm)with 0.2% ammonium formate buffer salt solution-0.2% ammonium formate buffer salt methanol solution as the mobile phase for gradient elution. The column temperature was maintained at 30℃,and the analytes were determined by a positive and negative ionization electrospray mass spectrometer with electro-spray ionization source. Result: The 47 chemical constituents including 26 indole alkaloids,15 organic phenolic acids and glycosides,2 isoquinoline alkaloids,2 triterpenes,1 flavonoids and 1 iridoid glycoside were identified from the aqueous extract of N. officinalis or tentatively characterized by comparing their mass spectra information with those of reference substances and literature data. Nine compounds were reported for the first time from N. officinalis,namely quinic acid,3,4-dihydroxy cinnamic acid,barbinervic acid,nauclefiline,vanillic acid,3, 4,5-trimethoxy phenol,3,4-dihydroxy benzoic acid methyl ester,2,3-dihydroxy benzoic acid amide and naucleactonin A isomers. Conclusion: In this study,the main chemical components from the aqueous extract of N. officinalis were rapidly identified as indole alkaloids,organic phenolic acids and glycosides by using UPLC-Q-TOF-MS/MS. Nine compounds were reported from N. officinalis for the first time.关键词：Nauclea officinalis;aqueous extract;UPLC-Q-TOF-MS/MS;indole alkaloids;quinoline alkaloids10|525|5更新时间：2024-01-04
- 摘要：Objective: To establish an LC-MS/MS method for simultaneous determination of the four effective components(paederosidic acid, paederoside,paederosidic acid methyl ester,and asperuloside) in Paederia scandens extracts. Method: A Venusil MP C18 column (2.10 mm×50 mm,5 μm)was used,with methanol(A)-water(B) as the mobile phase for gradient elution(0-0.3 min,10% A;0.3-0.8 min,10%-95%A;0.8-3.0 min,95%A;3.0-3.2 min,95%-10%A;3.2-4.5 min,10% A). The flow rate was 0.35 mL·min-1, the column temperature was room temperature, and the injection volume was 10 μL. Electrospray ionization(ESI) source was used. Quantization was performed using multiple reaction monitoring(MRM) mode and positive ionization condition. Mass spectrometric parameters of spectrometer and 4 effective components were optimized for testing and quantitative analysis. Result: Based on the instrument precision,linear range,repeatability,stability,recovery and other methodological validations,the results were in conformity with relevant standards of quantitative analysis. The calibration curves of paederosidic acid,paederosidic,methyl paederosidate and asperuloside showed a good linearity (r ≥ 0.994 7).The average recoveries of these four components were 90.8%-112.1%,with RSDs (n=6) of no more than 5.1%. Conclusion: The method is simple, rapid, accurate and reliable,and suitable for the quantitative analysis of four effective components in the extract of P. scandens.关键词：paederosidic acid;paederoside;paederosidic acid methyl ester;asperuloside;paederia scandens extracts;content determination;LC-MS/MS8|263|5更新时间：2024-01-04
- 摘要：Objective: To establish a liquid chromatography-mass spectrometry (LC-MS/MS) method for content determination of ten compounds including kaempferol-3-O-β-D-glucoside,diphyllin-4-O-β-D-glucoside,diphyllin-4-O-β-D-glucopyranosyl-(1→2)-β-D-arabinopyranoside,podophyllotoxin-4-O-β-D-glucoside,4'-demethylpodophyllotoxin,kaempferol,podophyllotoxone,diphyllin,quercetin,and podophyllotoxin from Diphylleia sinensis in different batches, analyze the similarities and differences in the chemical constituents between different batches of samples,and provide a new scientific basis for establishing a comprehensive evaluation system on the quality of D. sinensis. Method: The separation was performed on COSMOSIL-C18 column (4.6 mm×250 mm,5 μm) at 30℃ with a flow rate of 0.3 mL·min-1, with acetonitrile-0.1% formic acid solution as the mobile phase for gradient elution (0-30 min,30% acetonitrile;30-40 min,30%-32% acetonitrile.40.0-40.5 min,32%-50% acetonitrile;40.5-50.0 min,50%-60% acetonitrile;50-85 min,60% acetonitrile;85.0-85.5 min,60%-30% acetonitrile;85.5-95.0 min,30% acetonitrile),and the detection wavelength was set at 300 nm. The analyte was monitored by positive electrospray ionization(ESI) in multiple reaction monitoring mode to determine the content of ten compounds in different batches of D. sinensis. Result: Ten components showed good linear relationships within the investigated concentration ranges(r>0.999 1) as follows:0.07-2.23,0.39-12.40,0.05-1.68,0.12-3.88,0.05-1.73,0.08-2.70,0.05-1.00,0.04-1.10,0.04-0.58,and 0.18-5.67 mg·L-1,with RSDs of the precision,repeatability and stability less than 2%,and the average recovery rate was in the range of 98.61%-99.93% respectively. Significant differences were found in the chemical constituents of the D. sinensis between different batches. Conclusion: The method is rapid and sensitive with high precision,and it can be used to detect the content of components in D. sinensis from different batches. This method would provide important data for establishing and completing the medicinal quality comprehensive evaluation system ofD. sinensis.关键词：Diphylleia sinensis;LC-MS/MS;content determination8|221|2更新时间：2024-01-04
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Pollution and Distribution Characteristics of Heavy Metals in Different Parts of in Guizhou Province
摘要：Objective: To investigate the pollution and distribution characteristics of heavy metals in different parts of Rosa roxburghii. Method: The contents of heavy metals in different parts of R. roxburghii were determined by atomic absorption spectroscopy and atomic fluorescence spectrometer. Then the contents of heavy metals were evaluated by using bioconcentration factors(BCF)method,pollution index method,and correlation analysis. Result: The standard curves of Pb,As,Hg and Cd (heavy metals) were Y=373.54X-2.168 7(0-100 μg·L-1),Y=120.184X+1.626(0-100 μg·L-1),Y=742.222X-16.301(0-2 μg·L-1),and Y=0.023 3X+0.017 9(0-10 μg·L-1)respectively(r ≥ 0.999). Among 131 samples of R. roxburghii (including 15 batches of root,48 batches of stem,48 batches of leaf,and 20 batches of fruit),25 batches of samples exceeded the standard of heavy metals based on the limits of heavy metals in Green Standards of Medicinal Plants and Preparations for Foreign Trade and Economy(WM/T2-2004), including 16 batches of Cd,4 batches of Hg,4 batches of Pb and 1 batch of As. The single factor pollution index of heavy metals was Cd>Pb>Hg>As. The comprehensive pollution index in different parts of R. roxburghii was root > stem > leaf > fruit. The average BCF was Cd > Hg > Pb≈As. The average contents of heavy metals (Pb,As,Hg,and Cd) in 45 batches of soil samples in R. roxburghii base were all lower than the limit values in Grade Ⅱ national standard for soils,complying with the requirements on the limit values of heavy metals in base soils for the cultivation of Chinese herbal medicines. Conclusion: The Cd pollution of R. roxburghii mainly comes from soil,while Hg,Pb,and As may be related to atmospheric deposition and agricultural activities.关键词：Rosa roxburghii;heavy metals;pollution index;distribution characteristics9|188|5更新时间：2024-01-04 - 摘要：Objective: To investigate the correlation between the quality and the contents of inorganic elements in Paeoniae Radix Alba in Bozhou. Method: The contents of nineteen elements,such as Zn,Mn,K,Fe,Ca,Mg,Cu,Cd in Paeoniae Radix Alba were collected from twelve towns in Bozhou, the corresponding soil were determined using inductively coupled plasma mass spectrometry (ICP-MS) and the contents of gallic acid,paeoniflorin and paeonolide were determined by using high performance liquid chromatography(HPLC). In addition,the contents of water-soluble extractives were also determined, and the correlation between them was analyzed by using SPSS 19.0 software. Result: In the twelve towns,Paeoniae Radix Alba had high contents of six kinds of inorganic elements,namely Fe,Mn,Zn,K,Ca and Mg. Among them,the contents of K,Ca and Na were the highest,the contents of Fe and Mn were the second,and the content of Zn was the lowest. The contents of Cr,Hg,Cd and Cu in soil were significantly correlated with the contents of inorganic elements, and the contents of other elements,such as Ca,Al,Ti and Mn, were significantly correlated with other elements in Paeoniae Radix Alba in Bozhou,while other inorganic elements in soil were not correlated. The content of Na in soil had the greatest impact on the quality of Paeoniae Radix Alba in Bozhou,and it was negatively correlated with the contents of gallic acid,paeoniflorin,paeonolide,benzoic acid,benzoyl paeoniflorin and water-soluble extractives. The contents of heavy metal elements were low,which was within the range on the basis of the 2015 Chinese Pharmacopoeia. Conclusion: By analyzing the correlation between the quality and the contents of inorganic elements in Paeoniae Radix Alba in Bozhou and the contents of the inorganic elements in corresponding soil,we can provide basic data and reference for the future choice of production areas and the construction of GAP bases of Paeoniae Radix Alba in Bozhou.关键词：Paeoniae Radix Alba in Bozhou;soil;inorganic elements;correlation10|227|4更新时间：2024-01-04
- 摘要：Objective: To establish an HPLC fingerprint spectrum of decoction pieces-standard decoction-dispensing granules of Dictamni Cortex and evaluate the correlation among them. Method: Gradient elution was performed with mobile phase of acetonitrile-water,detection wavelength was set at 228 nm,flow rate was 1.0 mL·min-1,and column temperature was 30℃.The correlation of decoction pieces-standard decoction-dispensing granules of Dictamni Cortex was evaluated through the control fingerprint spectrum. Result: There were 10 common peaks in all fingerprint spectra of 15 batches of decoction pieces-standard decoction-dispensing granules of Dictamni Cortex with a good correlation.Besides,in the 10 common peaks,three of them were dictamnine,obacunone and fraxinellone. Conclusion: The study establishes an HPLC fingerprint spectrum,and it reflects the whole picture of multi-component of decoction pieces-standard decoction-dispensing granules of Dictamni Cortex.It can provide the reference for establishing quality standard and quality control of dispensing granules of Dictamni Cortex.关键词：Dictamni Cortex;fingerprint;dispensing granules;dictamnine;obacunone;fraxinellone8|428|4更新时间：2024-01-04
- 摘要：Objective: To identify Spatholobi Caulis extract with thin layer chromatography(TLC) and determine total content of catechin and epicatechin in it with high performance liquid chromatography(HPLC),in order to provide a reference for the quality control of Spatholobi Caulis. Method: TLC was put into effect with 0.7% sodium carboxymethylcellulose(CMC-Na) silica gel G as adsorbent,chloroform-acetone-(n -butanol)-methanol-formic acid(67:13:14:3:3) as developing solvent and 5% vanillin sulfuric acid solution as chromogenic agent.HPLC was carried out with the Kinetex C18 column(4.6 mm×250 mm,5 μm) and methanol-0.02% phosphoric acid water solution as mobile phase to perform gradient elution;the flow rate was 1.0 mL·min-1,the column temperature was 25℃,the injection volume was 5 μL,and the detection wavelength was 280 nm. Result: In the TLC,the color and shape of the extract spots were the same as those of catechin and epicatechin references at the corresponding positions,and those spots were clear and well separated.In the quantitative analysis of HPLC,catechin and epicatechin had good linear relationships in the range of 9.9-198.8 mg·L-1 and 20.6-412.4 mg·L-1,respectively;and their correlation coefficients were all 0.999 9. The average recoveries of catechin and epicatechin was 98.12% and 97.94% with RSD of 1.7% and 1.4%,respectively. Conclusion: The TLC established in this experiment has strong specificity and good separating degree.The HPLC which was used to determine total content of catechin and epicatechin in the extract has high accuracy and good repeatability.The established methods can be used to control quality of Spatholobi Caulis extract.关键词：Spatholobi Caulis;extract;thin layer chromatography;catechin;epicatechin;content determination10|459|3更新时间：2024-01-04
- 摘要：Objective: To investigate the underlying mechanism of the total flavonoids from Jinhe Yangxin prescription (FJYP) on myocardial ischemia (MI) injury induced by isoproterenol (ISO) in mice. Method: Totally 108 SPF KM mice were randomly divided into blank group, model group, positive group (propranolol hydrochloride, 33 mg·kg-1), high-dose FJYP group (4.4 g·kg-1), middle-dose FJYP group (2.2 g·kg-1), and low-dose FJYP group (1.1 g·kg-1). All mice were administrated with drugs for 7 days, except for the blank group. All mice were injected subcutaneously with ISO on the 5th, 6th and 7th day to establish the MI model. The electrocardiogram test was conducted in anesthetized mice before the first injection and after the final injection with ISO. The heart weight index (HWI) was determined. Triphenyltetrazolium chloride(TTC)staining was performed to determine the area of MI. Creatine kinase isoenzyme MB(CK-MB), cardiac troponin I(cTnI), lactate dehydrogenase(LDH), alanine aminotransferase(ALT), aspartate aminotransferase(AST), superoxide dismutase(SOD), malondialdehyde(MDA), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α) and IL-1β levels in serum were measured using commercial kits, and the pathological changes of myocardial tissues were observed by hematoxylin-eosin (HE) staining. Western blot analysis was used to detect the expression levels of nucleotide binding oligomeric domain-like receptor protein 3(NLRP3), apoptosis related speckle(ASC), Caspase-1. Result: Compared with the blank group, ST-segment was significantly elevated in model group (P<0.01). The heart weight index, CK-MB, cTnI, ALT, AST, LDH, MDA, IL-6, TNF-α and IL-1β in serum were significantly increased, and serum SOD activity was significantly decreased (P<0.01); the MI area was increased (P<0.01). The expression levels of NLRP3, ASC, Caspase-1 were all increased (P<0.01). Compared with the model group, FJYP could effectively decrease the change of ST-segment (P<0.05, P<0.01). The heart weight index, CK-MB, cTnI, ALT, AST, LDH, MDA, IL-6, TNF-α, IL-1β and the MI area of mice decreased, while the serum SOD content increased (P<0.05,P<0.01); and the pathological damages induced by ISO were significantly alleviated in FJYP treatment group; and the expression levels of NLRP3, ASC, Caspase-1 were also decreased (P<0.05, P<0.01). Conclusion: FJYP plays a protective role in myocardial ischemia in mice, the protective effect and mechanism may be related to increase of antioxidant ability, reduction of oxidant damage and inhibition of the NLRP3 inflammasome activation.关键词：total flavonoids from Jinhe Yangxin prescription;myocardial ischemia;oxidation;nucleotide binding oligomeric domain-like receptor protein 3(NLRP3);inflammation10|270|5更新时间：2024-01-04
- 摘要：Objective: To investigate the anti-atherosclerotic mechanism of emodin from Polygonum multiflori Radix Praeparata and the effect of Janus kinase 2(JAK2)/signal transduction and activator of transcription 3(STAT3) signaling pathway, and its related factor suppressor of cytokine signaling 3(SOCS3). Method: Eighty male ApoE-/-mice were randomly divided into 8 groups:emodin high, middle, and low dose groups(40,20,10 mg·kg-1), model group, positive control group(Xuezhikang,200 mg·kg-1), negative control group(tween-80,4 mg·kg-1), normal control group, and middle dose of emodin+AG490 group (DA group). In addition to the normal control group, the remaining 7 groups were fed with high-fat diet and subcutaneously injected with lipopolysaccharide, then stopped after 9 weeks. Mice were dosed on the 10th week and sacrificed 6 weeks later. The contents of JAK2, p-JAK2, STAT3, p-STAT3 and SOCS3 were measured by enzyme linked immunosorbent assay(ELISA). The thoracic aorta was stained by hematoxylin and eosin(HE). The expression of JAK2, STAT3 and SOCS3 mRNA was detected by Real-time PCR. Result: Compared with the model group, SOCS3 content increased (P<0.01), p-JAK2, and p-STAT3 levels decreased(P<0.01) in the emodin high-and middle-dose group, and there was increased in low-dose (P<0.05). There was no change in STAT3,JAK2. The effect of DA in each index was not as good as that in moderate dose group (P<0.01), but the relative expression of STAT3 in middle dose group was significantly different from that in DA group (P<0.05). The trend of SOCS3 gene expression in each group was increased to that detected by Real-time PCR and the results of JAK2 and STAT3 gene expression were decreased.Under the HE staining microscope, high and middle doses of emodin can effectively delay the formation of atherosclerotic plaque, but the effect of low dose is not obvious. Conclusion: Emodin can significantly affect the development of atherosclerotic lesions in ApoE-/- mice. This mechanism may be related to the inhibition of JAK2/STAT3 signaling pathway.关键词：atherosclerosis;emodin;Janus kinase 2(JAK2)/signal transduction and activator of transcription 3(STAT3) signaling pathway;suppressor of cytokine signaling 3(SOCS3)14|350|9更新时间：2024-01-04
- 摘要：Objective: To observe the effect of Shihu compound(SC)on expression levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in renal tissues of db/db diabetic mice. Method: The 11-12 week old male db/db mice, were randomly divided into model group, glipizide treatment group, low and high dose SC groups, and db/m normal control group according to fasting blood glucose and body mass. Intragastric administration was given for 8 weeks. Body weight and 24 hours urinary protein quantitation in mice were detected; the pathological changes of renal tissues were observed by optical microscope. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of TNF-α and IL-6 in serum and kidney tissue of mice; immunohistochemical staining and Western bolt were used to detect the mRNA and protein expression levels of TNF-α and IL-6 in renal tissue. Result: SC can significantly reduce the body weight (P<0.05, P<0.01) and 24 h urinary protein (P<0.05, P<0.01) in db/db mice. Hematoxylin and eosin(HE) staining results showed that the volume of glomerulus was increased, mesangial matrix was increased, mesangial area was widened and vacuolar degeneration of renal tubular epithelial cells was obvious in model group. As compared with the model group, the glomerular basement membrane was thinner, the proliferation of mesangial cells was decreased, the extracellular matrix was decreased, and the renal tubule structure was basically recovered to a normal condition. The results of immunohistochemistry, ELISA and Western bolt showed that the expression levels of TNF-α and IL-6 in the model group were increased significantly as compared with the normal group (P<0.01); as compared with the model group, the expression levels of TNF-α and IL-6 in each treatment group were significantly decreased (P<0.05, P<0.01), but still higher than those in the normal group. Conclusion: SC can alleviate the inflammatory reaction in kidney tissues and improve the renal damage in db/db mice by decreasing the expression of TNF-α and IL-6, thereby slowing down the progress of diabetic nephropathy.关键词：Shihu compound;db/db mice;tumor necrosis factor-α;interleukin-69|303|9更新时间：2024-01-04
- 摘要：Objective: To investigate the effects of Zhuanggu Shenjin capsule (ZGSJC)on bone histomorphometry and the expression of type I collagen protein in ovariectomized rats(OVX). And then to explore the mechanism of treating osteoporosis with ZGSJC. Method: 72 female SD rata were randomly divided into 6 groups after ovariectomy:sham group, model group, positive control group and ZGSJC treatment group with doses of 5.42, 2.71, 1.36 g·kg-1. Each group included 12 rats. After treated 3 moths, right tibia were dissected and undecalcified bone slice were made for bone histomorphometry detection, percentage of trabecular volume(TBV),bone trabecular absorption surface percentage(TRS),Bone trabecular surface percentage(TFS),average osteoid width(OSW),bone trabecular mineralization rate(MAR),bone cortical mineralization rate(mAR) were recorded. Used left tibia to extract protein and the expression of type I collagen protein was analyzed by Western blot. Result: In contrast with sham rats, OVX rats showed significant decreased in tibia TVB%, significant increase in tibia TRS%, TFS%, MAR, OSW and mAR. And showed significant decrease in type I collagen protein expression; compared with model rats, ZGSJC can inhibit the decrease of TVB% in tibia, inhibit the increase of TRS%, TFS%, MAR, OSW and mAR in tibia and inhibit the decrease expression of type I collagen protein. Conclusion: ZGSJC has the therapeutic effect in OVX Osteoporosis which could regulate bone great rate of transformation in OVX Osteoporosis; ZGSJC can increase the expression of type I collagen protein,which are the mechanisms that ZGSJC can be treated for Osteoporosis.关键词：Zhuanggu Shenjin capsule;osteoporosis;type I collagen;bone histomorphometry8|124|1更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of Shenling Baizhu San (SBS) on the proliferation and apoptosis of human colon cancer HCT116 cells. Method: The growth of HCT116 cell lines treated with SBS was investigated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT) assay, the cell cycle distribution was analyzed by flow cytometry propidium iodide (PI) staining, and its apoptosis was monitored by Annexin V-FITC kit.B-cell lymphoma-2(Bcl-2) and Bcl-2 associated X protein(Bax) were monitored by Westem blot(WB). Result: ① MTT results showed that the inhibition rates of HCT116 cells treated by the groups of 0.002, 0.004, 0.008, 0.016, 0.032, 0.064,0.128 g·L-1oxaliplatin combined with 5%SBS serum respectively were all significantly increased compared with those uncombined with 5%SBS serum after 48 hours (P<0.05). The inhibition rate of the group of 0.064 g·L-1in L-OHP combined with 5%SBS serum was the highest or (94.95±0.90)%. ② Cell cycle results showed that the distributions of HCT116 cells were all increased in G2/M phase, which were transferred from G0/G1,S and G2/M phases to G2/M phase after the combined administration of low, medium and high-dose oxaliplatin (0.002, 0.004, 0.008 g·L-1) with 5%SBS serum respectively, and the high-dose oxaliplatin combined 5%SBS serum was the most significant (P<0.05). ③Apoptosis results showed that the apoptosis of HCT116 cells could be induced by low, medium and high-dose oxaliplatin before and after being combined with 5%SBS serum respectively (P<0.05), and the high-dose combination group showed the highest apoptosis rate or (24.97 ±2.45)%. ④ WB results showed that compared with the 5%blank serum group, the expression of Bax protein was significantly up-regulated after the combined administration of low, medium and high-dose L-OHP with 5%SBS respectively (P<0.05). Compared with blank serum group and blank control group, the expression of Bcl-2 protein in each drug group was significantly decreased (P<0.05), and the high-dose combination group showed the lowest expression. Conclusion: SBS could inhibit the proliferation and apoptosis of HCT116 cell lines when being combined with oxaliplatin. The mechanism may be achieved by inhibiting the proliferation, inducing the cell apoptosis, changing the cell cycle distribution, up-regulating the Bax protein expression and down-regulating the Bcl-2 protein expression.关键词：colon cancer;Shenling Baizhu San;oxaliplatin;proliferation;cell cycle;apoptosis12|219|4更新时间：2024-01-04
- 摘要：Objective: To investigate the effects of capsaicin on the migration and invasion of hepatoma SMMC-7721 cells and explore its possible molecular mechanism. Method: Different concentrations of capsaicin groups and blank group were established, and the cell viability was detected by cell counting kit-8 method 24 h after capsaicin (0,25,50,75,100,150,200,250 μmol·L-1) treatment on hepatoma SMMC-7721 cells. The effect of capsaicin (0,50,75,100 μmol·L-1) on cell migration and invasion ability of SMMC-7721 cells was detected by transwell. Western blot analysis was used to detect the protein expression levels of E-cadherin, Vimentin, matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9) in cells treated with capsaicin(0,50,75,100 μmol·L-1)for 24 h. Result: As compared with the blank group, capsaicin(25,50,75,100 μmol·L-1)had no significant effect on cell survival rates after 24 h, and starting from 150 μmol·L-1, the cell survival rate was gradually decreased as the concentration of capsaicin was gradually increased (P<0.01), showing a dose-dependent effect. As compared with the blank group, capsaicin(50,75,100 μmol·L-1)intervention significantly inhibited migration and invasion of hepatoma SMMC-7721 cells in a concentration-dependent manner (P<0.01). As compared with the blank control group, capsaicin(50,75,100 μmol·L-1)intervention significantly up-regulated the E-cadherin protein expression level in SMMC-7721 cells (P<0.01), down-regulated Vimentin, MMP-2 and MMP-9 protein expression levels (P<0.01). Conclusion: Capsaicin can inhibit the migration and invasion of hepatoma SMMC-7721 cells, and the mechanism may be related to up-regulating the expression of E-cadherin and down-regulating the expressions of Vimentin, MMP-2 and MMP-9 in SMMC-7721 cells.关键词：capsaicin;SMMC-7721 cells;cell migration and invasion;E-cadherin;Vimentin;matrix metalloproteinase(MMP)-2;MMP-98|167|6更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of curcumol combined with 5-fluorouracil (5-Fu) on the proliferation, apoptosis and the changes in the expressions of proliferating cell nuclear antigen (PCNA), B cell lymphoma-2 (Bcl-2) in colorectal carcinoma cell line LoVo. Method: LoVo cells were treated with curcumol (50 mg·L-1) and/or 5-Fu (2 mg·L-1). The proliferation of LoVo cells were detected by methylthiazolyldiphenyl-tetrazolium bromide (MTT)assay. The apoptosis were analyzed by flow cytometer. The changes in PCNA and Bcl-2 expressions were detected by Western blot. Result: Curcumol, 5-Fu and combination group could inhibit the proliferation and promote the apoptosis of LoVo cells. The rates of inhibition and apoptosis were significantly different from control group (P<0.05). And the expressions of PCNA and Bcl-2 were obviously decreased, with a significant difference from control group (P<0.05). After combined treatment, the promoting effect of apoptosis was more obvious than single treatment group (P<0.05), and the expressions of PCNA and Bcl-2 protein were significantly decreased, with a significant difference from control group (P<0.05). Conclusion: Curcumol combined with 5-Fu could effectively inhibit the proliferation and promote the apoptosis of LoVo cells. Compared with the single treatment group, the combination of curcumol and 5-Fu can increase the sensitivity of cancer cells and greatly promote the apoptosis of LoVo cells. These mechanisms may be related to the down-regulation of PCNA and Bcl-2 protein expressions.关键词：curcumol;5-fluorouracil(5-FU);colorectal carcinoma;proliferation and apoptosis;proliferating cell nuclear antigen;B cell lymphoma-28|196|3更新时间：2024-01-04
- 摘要：Objective: To establish UPLC-MS analysis method for the determination of dendrobine in the main organs of C57BL/6J mice,and explore the tissue distribution characteristics of dendrobine in vivo. Method: At 0,30,60,120 min after gavage,the main organs (heart,liver,spleen,lung,kidney,brain and intestine) were collected and precipitated by acetonitrile,and the supernatant was detected with UPLC-MS.The chromatography separation was performed on a Hypersil Gold C18 column(2.1 mm×150 mm,1.9 μm) with acetonitrile-0.1% formic acid in water for gradient elution.Heated electrospray ionization source(HESI) was applied and operated in the positive ion mode.Meanwhile,pseudoephedrine hydrochloride was used as the internal standard for determining. Result: The endogenous substances in the tissues of mice had no interference for the assay of dendrobine,and the methodological study met the requirements.Dendrobine could distribute in heart,liver,spleen,lung,kidney,brain and intestine tissue of mice,and its content reached the peak of content at 30 min after gavage,and then decreased significantly with time.The distributions of dendrobine at different time points after administration were distinct.Dendrobine was mainly distributed in liver tissue at 30 min (1 241.69±78.64) μg·L-1, and in jejunum and ileum at 60,120 min (166.15±10.80) μg·L-1 and (118.25±8.51) μg·L-1,respectively. Conclusion: Dendrobine is widely distributed in mice,and its concentration in intestinal tissue and liver is high.The distribution in brain suggests that dendrobine can penetrate the blood-brain barrier,and this study can provide a basis for elucidating its neuropharmacological action.关键词：Dendrobium nobile;dendrobine;tissue distribution;pseudoephedrine hydrochloride;methodological investigation;live;ultra-performance liquid chromatography-tandem mass spectrometry8|322|4更新时间：2024-01-04
- 摘要：Objective: To study on the spectrum-effect relationship between UPLC fingerprint and anti-inflammatory of different extracts of Chuanxiong Rhizoma,in order to determine the active ingredients of this herb and describe its network regulatory mechanism. Method: UPLC-Q-TOF/MS was used to analyze the components in 8 different extracts of Chuanxiong Rhizoma.The anti-inflammatory effect of extracts was investigated by human bronchial epithelial cells.The spectrum-effect relationship was established by grey correlation analysis(GRA) and partial least squares repression analysis(PLSR).Molecular docking technology was adopted to predict the targets of active components in Chuanxiong Rhizoma,and Kyoto Encyclopedia of Genes and Genomes(KEGG) database was employed to analyze the pathways in order to explain the anti-inflammatory mechanism of this herb. Result: The 95% ethanol extract,75% ethanol extract,50% ethanol extract,25% ethanol extract,crystal,ligarine extract and chloroform extract all had anti-inflammatory effect on inflammatory cells,and 95% ethanol extract of Chuanxiong Rhizoma was the strongest.Three anti-inflammatory components were senkyunolide A,Z-ligustilide and neocnidilide.Z-ligustilide may inhibit the expression of downstream proteins by inhibiting the signal transduction to play anti-inflammatory effect through cyclooxygenase-2(COX-2),extracellular regulated protein kinase 2(EKR2),protein kinase C(PKC),Janus kinase 1(JAK1),JAK2,JAK3,nuclear transcription factor-кB inhibitory protein kinase β(IKKβ) and tumor necrosis factor-α(TNF-α);but signal channels of senkyunolide A and neocnidilide were COX-2,EKR2,PKC,phosphatidylinositol 3-kinase-α(PI3K-α),PI3K-γ,JAK1,JAK2,JAK3,IKKβ and TNF-α. Conclusion: Senkyunolide A,Z-ligustilide and neocnidilide are active components with anti-inflammatory effect in Chuanxiong Rhizoma.They may inhibit the expression of downstream proteins by inhibiting the signal transduction to play anti-inflammatory effect through different targets,which reveals the main active components,targets and mechanism of Chuanxiong Rhizoma and provides a reference for further study of its pharmacological role.关键词：Chuanxiong Rhizoma;anti-inflammatory;spectrum-effect relationship;molecular docking technique;protein pathways;fingerprints;extract8|835|18更新时间：2024-01-04
- 摘要：Objective: To observe the clinical efficacy and part of mechanism of Duhuo Jisheng Tang combined with acupuncture in the treatment of wind cold dampness type knee osteoarthritis (KOA), in order to provide reference for clinical application. Method: Totally 91 patients with KOA were randomly divided into observation group (49 cases) and control group (42 cases). The control group was given gluconosulfate capsules, 0.5 g/time, 3 times/days. While the observation group was treated with Duhuo Jisheng Tang, 150 mL/time, 2 times/days, and the patients were treated with acupuncture at the knee. The course of treatment was 4 weeks. Before and after treatment, the changes in knee joint visual analogue scale (VAS), Xi'an Ontario McMaster University Osteoarthritis Index (WOMAC) score, traditional Chinese medicine (TCM) symptom score, self-made knee swelling score and comprehensive efficacy and treatment response score were observed, Real-time PCR was used to detect the mRNA expressions of Toll-like receptor-4 (TLR-4) and myeloid factor (MyD88) in synovial fluid of both groups before and after treatment, and the recurrence rates at 3rd and 6th months between both groups were compared. Result: After treatment, VAS, WOMAC, TCM symptom score, self-made knee swelling score and comprehensive efficacy, treatment response score of the two groups of patients were lower than before treatment. Compared with control group, the score was lower in observation group, with statistically significant differences (P<0.05). The expressions of TLR-4 and MyD88 mRNA in the synovial fluid of two groups were lower than those in control group (P<0.05). The total effective rate of observation group, the recurrence rate at 3rd and 6th months were lower than those of control group, with statistically significant differences (P<0.05). Conclusion: Duhuo Jisheng Tang combined with acupuncture has a good efficacy in the treatment of wind cold dampness type KOA, with a low recurrence rate. The mechanism of action may be correlated with the blocking of the TLR-4/MyD88 signaling pathway and the inhibition of the expression of metabolic factors in the downstream chondrocytes and the expression of inflammatory factors.关键词：Duhuo Jisheng Tang;acupuncture;knee osteoarthritis;wind cold dampness type;recurrence rate8|284|7更新时间：2024-01-04
- 摘要：Objective: To observe the efficacy of Duhuo Jisheng Tang combined with warm acupuncture in treating patients with wind-cold dampness-type knee osteoarthritis (KOA) and explore its effect on serum pain mediators, inflammatory mediators and leukotrienes. Method: The 94 patients with wind-cold dampness-type KOA were randomly divided into control group (30 cases), Duhuo Jisheng Tang group (34 cases), and the combined group (30 cases). The control group was given with glucosamine sulfate capsules 0.5 g/time, tid by oral administration. The patients in Duhuo Jisheng Tang group received Duhuo Jisheng Tang 150 mL/times, bid by oral administration. And the combined group additionally received warm acupuncture on the basis of Duhuo Jisheng Tang. The course of treatment was 4 weeks in all groups. The knee joint visual analogue scale (VAS), self-made knee joint swelling score and total effective rate were observed both before and after treatment; enzyme-linked immunosorbent assay (ELISA) was used to detect the serum pain mediators[prostaglandin E2(PGE2), substance P (SP), dopamine(DA), 5-hydroxytryptamine(5-HT)], inflammatory mediators[tumor necrosis factor-α (TNF-α), interleukin-1β(IL-1β)]and leukotrienes[leukotriene (LT)B4, LTC4, LTD4]in all groups. Result: After treatment, the VAS scores, knee swelling scores and the efficacy for pain in combined group were significantly higher than those in control group and Duhuo Jisheng Tang group (P<0.05). As compared with control group, the levels of serum PGE2, SP, DA, 5-HT, TNF-α, IL-1β and allogeneic leukotrienes we lower in the Duhuo Jisheng Tang group and combined group (P<0.05). The decreases in levels of serum PGE2, SP, DA, 5-HT, TNF-α, IL-1β, LTB4, LTC4 and LTD4 in combined group were more obvious than those in Duhuo Jisheng Tang group after treatment (P<0.05). Conclusion: Warm acupuncture combined with Duhuo Jisheng Tang could effectively treat the knee joint pain for wind-cold dampness-type KOA, and the mechanism may be related to the reduction of LTB4, LTC4, LTD4 and inflammatory mediators.关键词：Duhuo Jisheng Tang;knee osteoarthritis;warm acupuncture;pain;pain mediators10|393|10更新时间：2024-01-04
- 摘要：Objective: To observe the changes of serum lysine and β-cryptoxantin in patients with hepatorenal deficiency type knee osteoarthritis treated with Duhuo Jisheng Tang and its correlation with pain. Method: Totally 73 KOA patients diagnosed at the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine were randomly divided into observation group (42 cases) and control group (31 cases). The observation group was orally given Duhuo Jisheng Tang, 150 mL/times, 2 times/d, while the control group was orally given glucosamine sulfate, 0.5 g/times, 3 times/d. Both groups were treated for 4 weeks. The knee joint visual analogue scale (VAS) and tenderness value were used to evaluate the changes of pain before and after treatment. Serum levels of prostaglandin E2 (PGE2), substance P (SP), dopamine (DA) and serotonin (5-HT) were measured by enzyme-linked immunosorbent assay (ELISA). The content of lysine was detected by automatic amino acid analyzer, and the content of serum β-cryptoxantin was determined by HPLC. Pearson correlation test was used to analyze the correlation between lysine, β-cryptoxanthin and VAS scores, tenderness value, PGE2, SP, DA and 5-HT. Result: Compared with before treatment, patients in control group and observation group after treatment had higher VAS scores and lower tenderness (P<0.05). After treatment, the VAS score of the observation group was higher, and the pain value was lower than that of control group (P<0.05). Compared with before treatment, the levels of PGE2, SP, DA and 5-HT in control group and observation group decreased after treatment.Compared with control group, the serum levels of PGE2, SP, DA and 5-HT in observation group decreased significantly (P<0.05) after treatment. The serum levels of lysine and β-cryptoxanthin in the control group had no significant changes before and after treatment. The serum levels of lysine and β-cryptoxanthin in the observation group were significantly higher than those before treatment (P<0.05). Serum lysine and β-cryptoxanthin were positively correlated with tenderness before and after treatment in both groups (P<0.05), but negatively correlated with VAS, PGE2, SP, DA and 5-HT (P<0.05). Conclusion: Duhuo Jisheng Tang can significantly relieve the pain in treatment of KOA, which may be related to the increase of contents of lysine and β-cryptoxanthin.关键词：Duhuo Jisheng Tang;knee osteoarthritis;lysine;β-cryptoxanthin;pain medium8|163|2更新时间：2024-01-04
- 摘要：Objective: To investigate the clinical efficacy and mechanism of Taiyuanyin and Shoutaiwan in patients with recurrent recurrent spontaneous abortion. Method: A total of 96 patients with recurrent recurrent spontaneous abortion (URSA) from January 2015 to March 2016 in our hospital were selected and divided into control group and observation group according to the random number table method, 48 cases in each group. The control group received active immunotherapy, and the observation group was treated with Taiyuanyin and Shoutaiwan combined with active immunotherapy. The pregnancy success rate and pregnancy outcome were compared between these two groups. The mRNA expression levels of Th17 cells, Treg cells, inflammatory factors interleukin (IL)-4, IL-10, IL-17, Th17, Treg transcription factor RORγt and FoxP3 were measured and compared between two groups. The incidence of adverse reactions was also compared. Result: The repregnancy rate was 95.8%in control group and 97.9%in observation group, with no statistical difference between two groups. The success rate of pregnancy was 93.8%(45/48) in observation group, significantly higher than 77.1%(37/48) in control group (P<0.05). The percentage of term delivery was 95.6%in observation group, significantly higher than 81.1%in control group (P <0.05). The ratios of Th17/CD4+ and Th17/Treg after treatment were significantly decreased in both groups, and Treg/CD4+was significantly increased (P<0.05), and the changes in observation group were more obvious than those in control group (P<0.05). After treatment, the levels of IL-4 and IL-10 were increased in both groups, and the level of IL-17 was decreased (P<0.05). After treatment, the levels of IL-4 and IL-10 in observation group were higher than those in control group, and the level of IL-17 was lower than that in control group (P<0.05). The expression level of RORγt mRNA was reduced and the expression level of FoxP3 mRNA was increased after treatment in both groups (P<0.05), and the changes in observation group were more obvious than those in control group (P<0.05). No adverse reactions were found in any patient. Conclusion: Taiyuanyin and Shoutaiwan combined with active immunotherapy for unexplained recurrent spontaneous abortion has clear efficacy. It can improve the imbalance of Th17/Treg by regulating the level of inflammatory factors, thus improving the outcome of pregnancy and reducing the abortion rate. In addition, it has high safety and low cost, worthy of clinical application.关键词：recurrent abortion;Taiyuanyin and Shoutaiwan;active immunization;helper T lymphocytes;interleukin(IL);retinoic acid-related solitary nucleus receptor γt9|259|5更新时间：2024-01-04
- 摘要：Objective: To observe the clinical efficacy and effect of Jinlida granule combined with Tongxinluo capsule on traditional Chinese medicine (TCM) syndromes, glucose metabolism, lipid metabolism and levels of interleukin-6(IL-6) in serum of patients with non-proliferative retinopathy of type 2 diabetes. Method: The 62 patients with non-proliferative retinopathy of type 2 diabetes were randomly divided into two groups, and all of them were given with basic treatment. The control group (32 patients) were treated with calcium hydroxybenzene sulfonate capsule, and the treatment group (30 patients) were treated with Jinlida granule combined with Tongxinluo capsule on the basis of control group. After 12 weeks, clinical efficacy, TCM syndromes improvement, fasting plasma glucose (FPG), 2 h postprandial blood glucose (2 hPG), glycosylated hemoglobin (HbA1c), triacylglycerol (TG), total cholesterol (TC), high-density lipoprotein-C (HDL-C), low-density lipoprotein-C (LDL-C), insulin resistance index (HOMA-IR) and IL-6 in two groups were observed and compared. Result: The clinical efficacy and the improvement of TCM syndromes in treatment group were significantly better than those in control group (P<0.05). The FPG, 2 hPG, HbA1c, TG, TC, HDL-C, insulin resistance index and IL-6 were significantly decreased, and the HDL-C was significantly increased in both groups after treatment, and the treatment group were better than control group (P<0.05). Conclusion: Jinlida granule combined with Tongxinluo capsule has a significant therapeutic effect on non-proliferative retinopathy of type 2 diabetes, and the mechanism may be related to regulating glucose and lipid metabolism, improving insulin resistance and reducing serum inflammatory factors.关键词：Jinlida granule;Tongxinluo capsule;diabetic retinopathy;glucose metabolism;lipid metabolism;inflammation8|141|3更新时间：2024-01-04
- 摘要：Objective: To study the clinical efficacy of Chaishao Chengqitang combined with low molecular heparin in treating hyperlipidemic pancreatitis (HLP) and the effect on inflammatory mediator, lipid and coagulation function. Method: Totally 167 cases of HLP patients treated in the Second Affiliated Hospital of Zhengzhou University from March 2014 to March 2016 were selected and divided into two groups according to the random number table, with 89 cases in clinical group and 88 in control group. Patients in both groups were treated with the routine therapy. In addition, the control group was also given low molecular heparin, while the clinical group was also given Chaishao Chengqitang. Then the treatment effect, clinical symptoms disappearance time, inflammatory mediator, lipid levels and coagulation function were compared. Result: Compared with 80.68% in the control group, the total effective rate of the clinical group was 93.25%. There was a significant difference (P<0.05). After treatment, the abdominal pain and distension disappearance time and gastrointestinal function, and blood urine amylase (AMY) recovery time were obviously shorter than those in control group. Compared with before treatment, interleukin-6(IL-6), C-reaction protein (CRP), tumor necrosis factor (TNF) -α of two groups after treatment decreased (P<0.05). Compared with control group, the values of TNF-α, IL-6, CRP and triglyceride (TG) in patients of clinical group were lower after treatment (P<0.05). After treatment, compared with before treatment, prothrombin time (PT) and activated partial thromboplastin time (APTT) of two groups were significantly increased. There were significant differences (P<0.05). Conclusion: Chaishao Chengqitang can significantly shorten the course of disease, reduce the expressions of serum inflammatory medium and blood lipid TG, and improve the patient's coagulative function. It has a better effect on HLP and is worth clinical application.关键词：Chaishao Chengqitang;hyperlipidemic pancreatitis;inflammatory mediator;lipid level;coagulation function;real hot junction10|166|3更新时间：2024-01-04
- 摘要：Objective: To observe the effect of addition and subtraction syndrome differentiation treatment of Dihuang Wan combined with Xionggui Erchen Tang on sex hormone levels and cytokines in patients with polycystic ovary syndrome hyperandrogenemia (PCOS-HA). Method: According to the order of medical treatment, one hundred and twenty patients were randomly divided into control group (60 cases) and observation group (60 cases) by random number table. Patients in control group got ethinylestradiol and cyproterone acetate tablets by oral administration from the fifth day of menstruation for 21 days, 1 time/day, then stopped the drug use to wait for menstrual onset. Patients in observation group got addition and subtraction syndrome differentiation treatment of Dihuang Wan combined with Xionggui Erchen Tang. The treatment course was 3 menstrual cycles in both groups. Before and after treatment, levels of serum testosterone (T), dihydrotestosterone (DHT), dehydroepiandrosterone sulfate (DHEAS), luteinizing hormone (LH), follicle stimulating hormone (FSH), sex hormone binding globulin (SHBG), and prolactin (PRL) were detected, and free testosterone indexes (FAI) were calculated. Scores of rosenfield, hairy and syndrome of kidney deficiency and blood stasis were graded. The size of ovary, number of antral follicles, recovery rate of ovulation were recorded in gynecologic B-ultrasound examination. Menstrual cycle, menstrual volume and basal body temperature (BBT) were recorded. And double phase rate of BBT and rate of recovery were calculated. Before and after treatment, levels of leptin (LP), insulin-like growth factor-1 (IGF-1) and adiponectin (APN) were detected. And homeostasis model assessment-insulin resistance index (HOMA-IR), body mass index (BMI) and waist-to-hipratio (WHR) were detected. Result: In the analysis of χ2test, the clinical efficacy in observation group was superior to that in control group (χ2=7.213, P<0.05). After treatment, the levels of T, DHT, DHEAS, FAI, LH, PRL, LH/FSH, IGF-1, LP, HOMA-IR, BMI and WHR in observation group were lower than those in control group, and levels of SHBG, FSH and APN were higher than those in control group (P<0.01). Scores of Rosenfield, hairy, score of syndrome of kidney deficiency and blood stasis and volume of ovary in observation group were lower than those in control group (P<0.01). Recovery rate of menstruation was 86.67% in observation group, higher than 68.33% in control group (χ2=5.784, P<0.05). Recovery rate of ovulation was 78.33% in observation group, higher than 56.67% in control group (χ2=6.419, P<0.05). Double phase rate of BBT was 80% in observation group, higher than 60% in control group (χ2=5.714, P<0.05). Conclusion: Addition and subtraction syndrome differentiation treatment of Dihuang Wan combined with Xionggui Erchen Tang can ameliorate clinical symptoms of hyperandrogenemia and serum biochemical index, regulate endocrine, and promote recovery of menstrual and spontaneous ovulation, superior to western medicine treatment in clinical efficacy.关键词：hyperandrogenism;polycystic ovary syndrome;syndrome of kidney deficiency and blood stasis;Dihuang Wan;Xionggui Erchen Tang;insulin-like growth factor-1;adiponectin;leptin9|257|2更新时间：2024-01-04
- 摘要：Objective: To observe the clinical efficacy of addition and subtraction treatment of Shenqi Shenqi Wan on overactive bladder (OAB) and investigate its effects on urodynamics, urine nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). Method: One hundred and eight patients were randomly divided into control group (54 cases) and observation group (54 cases) with the use of random number. In the control group, patients got Tolterodine 2 mg/time, 2 times/days. On the basis of treatment in the control group, the patients in observation group additionally got Shenqi Shenqi Wan, 1 dose/day. The treatment course was eight weeks in both groups. Daily urinating dairy (urination frequency in 24 h, nocturnal urination frequency, urinal urgency frequency, and urinal leakage frequency) was recorded. Before and after treatment, the symptoms of overactive bladder were assessed with overactive bladder symptom score (OABSS), Indevus Urgency Severity Scale (IUSSU), and the scores of main symptoms and kidney deficiency. Overactive bladder questionnaire (OAB-q) was used to evaluate the quality of life before and after treatment. Before and after treatment, the maximum flow rate (MFR), post-void residual volume (PVR), volume at urgent desire (VUD), volume at first desire (VFD), and other urodynamic indexes. NGF and BDNF were detected before and after treatment, and its ratio to urinary creatinine (UCr) (NGF/UCr) was calculated. Result: The total clinical efficacy was 92.59% in observation group, higher than 75.93% in control group (χ2=5.654, P<0.05). The urination frequency in 24 h, nocturnal urination frequency, urinal urgency frequency, and urinal leakage frequency in observation group were all less than those in control group (P<0.01). The scores of OABSS, IUSS, the main symptoms and kidney deficiency in observation group were all lower than those in control group (P<0.01). Scores of OAB-q scale were lower than those in control group, and the 4 dimension scores such as coping style were higher than those in control group (P<0.01). After treatment, the levels of NGF, BDNF, NGF/UCr and BDNF/UCr in the observation group were all lower than those in the control group (P<0.01). Conclusion: Addition and subtraction therapy of Shenqi Wan combined with Tolterodine can ameliorate clinical symptoms and detrusor function, improve quality of life, regulate urodynamic index as well as NGF and BDNF levels in the treatment of kidney deficiency type OAB.关键词：overactive bladder;kidney deficiency syndrome;Shenqi Wan;urodynamics;nerve growth factor;brain derived neurotrophic factor;quality of life9|134|1更新时间：2024-01-04
- 摘要：Objective: To investigate the material basis and predict the direction of action of Salviae Miltiorrhizae Radix et Rhizoma-Notoginseng Radix et Rhizoma paired drugs. Method: All the molecules, targets and related diseases for Salviae Miltiorrhizae Radix et Rhizoma and Notoginseng Radix et Rhizoma were retrieved from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Cytoscape 3.2.1 software was used to build the 'active ingredient-target' and 'target-related diseases' network model, and then the material basis and mechanism were studied and predicted. Result: Totally 73 active components, 145 targets and 325 related diseases were screened through oral bioavailability (OB) and drug likeness (DL) conditions. The first three active components with higher degree value included quercetin(87), beta-sitosterol(51) and 1,2,5,6-tetrahydrotanshinone(46). The first three targets with higher degree value included estrogen receptor (63), androgen receptor (59), and prostaglandin-endoperoxide synthase 2 (56). The related diseases with higher degree value included cancer (16), metastatic osteosarcoma in the lung (12), cardiovascular disease (10), breast cancer (9) and pancreatic cancer (9), etc. Conclusion: In this paper, the material basis and mechanism of Salviae Miltiorrhizae Radix et Rhizoma Notoginseng Radix et Rhizoma paired drugs were preliminarily predicted, providing a thinking direction for further research and clinical application.关键词：Salviae Miltiorrhizae Radix et Rhizome;Notoginseng Radix et Rhizoma;network pharmacology;paired drugs mechanism8|1053|14更新时间：2024-01-04
- 摘要：Objective: To study the relationship between effective ingredients, targets and pathway in Ermiaowan, and predict its possible"Multi-Efficacy of One Formula" Molecular Mechanism based on Network Pharmacology. Method: The main ingredients and targets of Ermiaowan were predicted and screened by relying on A Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine server(BATMAN-TCM). The Search Tool for the Retrieval of Interacting Genes/Proteins platform(STRING) was used to construct target-protein interaction network,and gene ontology(GO) bioprocess enrichment and clustering analysis of target genes were finished by using BiNGO and MCODE plug-ins. Database for Annotation,Visualization and Integrated Discovery(DAVID)was used to analyze kyoto Encyclopedia of Genes and Genomes(KEGG) signaling pathways of the key targets. Result: The active ingredients of Ermiaowan acted on 37 key targets such as serum albumin(ALB),mitogen-activated protein kinase1(MAPK1),tumour necrosis factor(TNF),prostaglandin G/H synthase 2(PTGS2),5-hydroxytryptamine receptor 2A(HTR2A),adenosine deaminase(ADA),glucocorticoid receptor(NR3C1).Got mainly involved in GO biological processes such as inflammation, nerve conduction, metal ions homeostasis, oxidation and reduction, lipid metabolism, and nucleotide metabolism; regulated signaling pathways such as TNF, TRP channel inflammatory, FcεRI, Type Ⅱ diabetes mellitus, calcium signaling pathway, serotonergic and a variety of signal transduction, tumor and cytochrome P450 enzyme. Conclusion: Besides Gout, arthritis, eczema and some inflammatory diseases,Ermiaowan may also have "Multi-Efficacy of One Formula" effect on diabetic peripheral neuropathy, nervous system, gastrointestinal system and cardiovascular system diseases.关键词：Ermiaowan;network pharmacology;cluster analysis;targets;pathways;protein-protein interaction9|775|8更新时间：2024-01-04
- 摘要：Objective: To investigate the molecular mechanism of ursolic acid (UA)against rheumatoid arthritis based on network pharmacology and molecular docking. Method: Potential therapeutic targets of UA were searched based on comparative toxicogenomics database(CTD), Drug Bank, traditional Chinese medicine systems pharmacology(TCMSP) and other databases. Pathway enrichment analysis of potential targets was conducted by DAVID(DAVID bioinformatics resources). Protein interaction was conducted by STRING, and energy matching between UA and 11 rheumatoid targets was done by molecular docking technique(iGEMDOCK, SystemsDock). The potential targets against rheumatoid arthritis were screened. Result: 66 corresponding targets were collected, and 27 signal pathways were obtained from the enrichment of DAVID pathway (P<0.01), including 11 key targets proteins in rheumatoid arthritis pathway (matrix metalloproteinase-1, matrix metalloproteinase-3, interleukin-6, transcription factor AP-1, tumor necrosis factor, vascular endothelial growth factor A, granulocyte-macrophage colony-stimulating factor, interleukin-1 beta, transforming growth factor beta-1, and proto-oncogene c-Fos). Through protein interaction, four target proteins were found to be supported by experimental data. The molecular docking showed that UA could resist rheumatoid arthritis mainly through matrix metalloproteinase-1, Matrix metalloproteinase-3, tumor necrosis factor, transcription factor AP-1 and interleukin-1 beta. Conclusion: The results of this study reveal the molecular mechanism of UA against rheumatoid arthritis。关键词：ursolic acid;network pharmacology;pathway analysis;rheumatoid arthritis;molecular docking9|1337|8更新时间：2024-01-04
- 摘要：With the change of people's life style and the improvement of living quality, hyperlipidemia has become a common clinical disease. At present, the treatment of hyperlipidemia is mainly based on drug therapy. As compared with western medicine, the mechanism of traditional Chinese medicine(TCM) for treating hyperlipidemia is complicated, but the adverse reactions are fewer. In order to explore its pathological pathogenesis, find prevention and treatment measures, and elucidate the advantages and features of TCM in treating hyperlipidemia, we need to establish an animal model that is similar to human hyperlipidemia. The animal model is the basis for experimental research, so the establishment of the animal model should be consistent with the development of the disease process, and the assessment indicators of the animal model should also correspond to the clinical indicators as far as possible. The ideal hyperlipidemia model should have the characteristics of repeatability, simple operation, low cost, and easy promotion. At present, there are many research methods for animal models of hyperlipidemia, and chemical induction is the most commonly used method. The experimental animals mainly include rats and mice, while the congenital models and transgenic models are rarely used. The biological characteristics of different animals are different, so their modeling methods are also different. According to the experimental objectives, period, techniques and other factors, selecting the right model animal and modeling method are the key to the success of hyperlipidemia experiments. This paper would summarize the recent experimental studies of hyperlipidemia animal models both at home and abroad in terms of animal selection, modeling methods and evaluation indicators of model, which will provide reference and basis for the hypolipidemic developers to study, choose and establish hyperlipidemia animal models.关键词：hyperlipidemia;model;indicator;research progress9|1552|12更新时间：2024-01-04
- 摘要：Andrographis paniculata,a member of the family Acanthaceae,with functions of clearing heat and detoxifying,cooling blood and eliminating swelling in traditional Chinese medicine,is commonly used for the treatment of fever,cold,throat sores,aphtha of the mouth and tongue,whooping cough,dysentery,diarrhea,pyretic stranguria pain,welling-abscess swellings and sores,snake bites and so on. Modern research shows that A. paniculata contains diterpenoids,flavonoids,phenylpropanoids,iridoids and alkaloids; the preliminary pharmacological research has shown that it has anti-inflammatory,antimicrobial,anti-tumor,antiviral,protecting cardiovascular,hypoglycemic,inhibiting platelet aggregation,protecting liver and other activities. A. paniculata is widely distributed in tropical and subtropical regions. It was introduced from southeast Asia to China in the 1950s and cultivated in Guangxi,Guangdong,Sichuan,Anhui and Fujian provinces. It has been included in many compound formulae due to its low price and significant pharmacological activities. In recent years,more and more researches are on the quality control of A. paniculata. The quality ingredients are changed from two components(andrographolide and dehydroandrographolide) to multiple components.The quality control methods also extend from thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC) to more accurate,rapid and reliable detection methods. In this review,the latest studies both at home and abroad about the herbal textual research,resource distribution,chemical constituents,pharmacological effects and quality analysis of A. paniculata would be reviewed and discussed in order to offer some reference for further studies and development.关键词：Andrographis paniculate;chemical components;pharmacological;quality analysis9|2110|24更新时间：2024-01-04
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