最新刊期
卷 24 ， 期 19 ， 2018
- 摘要：Uterine hypoplasia is a common gynecological disease,which is caused by low levels of hormones related to development process.The main symptoms are delayed menstruation,scanty menstruation,dysmenorrhea and even amenorrhea,and often manifested as infertility after marriage.The model of uterine hypoplasia is a pathological model with western medicine as the main index.Based on the analysis of characteristics of clinical symptoms of Chinese and western medicine and a large number of animal experiments,this article forms the following specifications (draft) for the preparation of uterine hypoplasia models (draft).关键词：uterine hypoplasia;animal model;specifications;draft;ovary;estrogen;dexamethasone sodium phosphate4|6|0更新时间：2024-01-04
- 摘要：Dry eye is one of the most common ophthalmic diseases in clinic.Its main manifestations are dry eyes,tears,foreign body sensation,blurred vision,asthenopia and so on.At present,the pathogenesis of dry eye is uncertain,the present dry eye animal model is mainly pathological model,and mainly based on western medicine indicators.Based on the analysis of the characteristics of clinical symptoms of Chinese and western medicine in dry eye and the large number of experimental studies on the existing animal models,this article forms the following specifications (draft) for the preparation of dry eye animal models.关键词：dry eye;animal models;specification;draft;benzalkonium chloride;nictitating membrane;break up time7|6|7更新时间：2024-01-04
- 摘要：Chronic prostatitis is a common multiple disease syndrome in medium-elderly men,and the animal models of chronic prostatitis are mainly the pathological models,which are mainly characterized by the diagnosis index of western medicine and the characteristics of traditional Chinese medicine syndrome.Based on the analysis of the characteristics of clinical symptoms of chronic prostatitis and a large number of animal experiments,this article forms the following specifications (draft) for the preparation of chronic prostatitis models (draft).关键词：chronic prostatitis;animal models;specification;draft;estrogen6|6|9更新时间：2024-01-04
- 摘要：In recent years,the incidence of prostatic hyperplasia shows a rising trend,and the age of onset tends to be younger.Most of the prostatic hyperplasia models are pathological with western medicine as the main indicators.Based on analysis of clinical characteristics of the Chinese and western medicine for prostatic hyperplasia,and a large number of experimental studies,this article forms the following specifications (draft) for the preparation of prostatic hyperplasia models (draft).关键词：prostatic hyperplasia;animal models;specifications;draft;testosterone propionate;sex hormone8|6|3更新时间：2024-01-04
- 摘要：Dysmenorrhea is a common gynecological disease,it usually occurs in women's menstrual period or go by before and after the emergence of the periodic abdominal pain,lumbosacral pain sometimes,there may be severe unbearable pain,accompanied by nausea and vomiting,dizziness syncope.The dysmenorrhea animal model is pathological model with mainly western medicine indexes.Based on the analysis of characteristics of clinical symptoms of Chinese and western medicine and a large number of animal experiments,this article forms the following specifications (draft) for the preparation of dysmenorrhea models (draft).关键词：dysmenorrhea;animal model;specifications;draft;oxytocin;estradiol benzoate;diethylstilbestrol7|6|18更新时间：2024-01-04
- 摘要：At present,infertility has become a common disease,more than 30% of couples of childbearing age need to get pregnant by medical means.Animal models of infertility are the basis for related studies.At present,the animal model of infertility is mainly based on the diagnostic criteria of western medicine,and taking into account the characteristics of traditonal Chinese medicine(TCM) syndromes.Based on the analysis of the clinical symptoms of infertility in TCM and western medicine and a large number of experimental studies on the existing animal models,this article forms the following specifications (draft) for preparation of female infertility models (draft).关键词：infertility;animal model;specifications;draft;immune factors;cyclophosphamide;polycystic ovary syndrome6|11|7更新时间：2024-01-04
- 摘要：Objective: To optimize the preparation process of curcumin-hydroxypropyl-β-cyclodextrin(CUR-HP-β-CD) inclusion complex and to evaluate its in vitro antitumor activity. Method: CUR-HP-β-CD inclusion complex was prepared by the procedure of ethanol-water cosolvent incubation-lyophilization;the preparation technology was optimized by orthogonal test with inclusion rate as index.CUR-HP-β-CD inclusion complex was characterized by ultraviolet spectrophotometry(UV) and fluorescence spectrophotometry.The cell viability of different types of cancer cells(human cervical cancer cell line HeLa cells and human hepatoma cell line HepG2 cells) was measured by microculture tetrazolium(MTT) assay through exposing cells to different concentrations of CUR-HP-β-CD inclusion complex for different times(24 h and 48 h). Result: The optimum process for preparation of CUR-HP-β-CD was as follows:the molar ratio of CUR to HP-β-CD of 1:3,the cosolvent ratio (absolute ethanol-water) of 1:9,the reaction temperature at 40℃ and the reaction time for 12 h;under these conditions,the inclusion rate was 42.2%,its lyophilized product had formed CUR-HP-β-CD inclusion complex.Both CUR-HP-β-CD inclusion complex and free CUR reduced the viability of HeLa and HepG2 cells in a dose-dependent manner,and their half-inhibitory concentrations were close,showing that CUR-HP-β-CD inclusion complex still exhibited significant cytotoxicity to cancer cells. Conclusion: The optimized technique is simple and reproducible.Besides,CUR-HP-β-CD inclusion complex retains the bioactivity of CUR.关键词：curcumin;hydroxypropyl-β-cyclodextrin;preparation process;inclusion complex;anticancer activity;microculture tetrazolium;inclusion rate8|5|5更新时间：2024-01-04
- 摘要：Objective: To investigate the chemical constituents of the underground parts of Liriope spicata var. prolifera, further enrich its chemical information and provide theoretical guidance for its development and utilization. Method: The underground parts of L. spicata var. prolifera were extracted by 60% ethanol. The chemical constituents in 60% ethanol extract were then isolated and purified by macroporous adsorption resin,positive and reverse silica gel,MCI gel and sephadex gel. Their structures were determined by detailed analysis of their physico-chemical properties and spectral data. Result: Twelve compounds were isolated and identified from the underground parts of L. spicata var. prolifera,including 4 flavonoids(compounds 1-4),4 phenols and benzoic acid derivatives (compounds 5-8),2 amide alkaloids (compounds 9, 10) and 2 other kinds (compounds 11, 12). Their structures were identified as 2',4,4'-trihydroxychalcone(1),luteolin(2),quercitroside(3),3,5-dihydroxy-7-methoxy-6-methyl-3-(4-hydroxy benzyl) chroman-4-one(4),2,6-dimethoxy-4-nitrophenol(5),allylpyrocatechol(6),3,4-dihydroxyallylbenzene 4-O-[α-L-rhamnopyranosyl(1→6)]-β-D-glucopyranoside(7),syringic acid(8),E- feruloyltyramine(9),tran-p-hydroxy cinnamic acid(10),(1 S,4 S,5 R,6 R,7 S,10 S)-1,4,6-trihydroxyeudesmane-6-O-β-D-glucopyranoside(11),and 5-hdroxymethyl-2-furaldehyde (12),respectively. Conclusion: Compounds 1-3, 5-8 and 10-12 were isolated from this genus for the first time. Compounds 4 and 9 were isolated from this plant for the first time.关键词：Liriope spicata var.prolifera;chemical constituents;structure identification;3,5-dihydroxy-7-methoxy-6-methyl-3-(4-hydroxy benzyl) chroman-4-one;E-feruloyltyramine6|7|3更新时间：2024-01-04
- 摘要：Objective: To develop an HPLC-CAD method for simultaneous determination of six ingredients in Gushukang capsule, namey naringin,epimedin B,epimedin C,icariin,5-hydroxymethylfurfural(5-HMF) and tanshinoneⅡA,in order to provide references for its quality control. Method: By HPLC-CAD,chromatographic separation was performed on a Agilent ZORBAX Eclipse XDB C18 column(4.6 mm×250 mm,5 μm), with acetonitrile (A)-0.05% formic acid (B) as the mobile phase for gradient elution(0-10 min,10%A;10-25 min,10%-25%A;25-30 min,25%-30%A;30-45 min,30%-60%A;45-50 min,60%-75%A),the flow rate was 0.8 mL·min-1,the nebulization temperature of detector was 35℃,the column temperature was maintained at 35℃,and the injection volume was 10 μL,so as to determine the 6 contents of Gushukang capsule. Result: Naringin, epimedin B,epimedin C,icariin,5-HMF,and tanshinoneⅡA were linear within the ranges of 0.006-0.120 μg(r=0.999 7),0.041-0.820 μg(r=0.999 9),0.011-0.220 μg(r=0.999 6),0.022-0.440 μg(r=0.999 8),0.012-0.240 μg(r=0.999 8) and 0.005-0.100 μg(r=0.999 8),respectively. The average recoveries were 99.26%,97.99%,98.17%,99.34%,98.26% and 99.25%,and the RSDs were 1.7%,1.0%,1.6%,1.4%,1.9% and 1.3%,respectively. Conclusion: The method is accurate,convenient,sensitive and reproducible for quality control of multicomponent in Gushukang capsule.关键词：Gushukang capsule;naringin;epimedin B;epimedin C;icariin;5-hydroxymethylfurfural (5-HMF);tanshinoneⅡA;content determination7|5|1更新时间：2024-01-04
- 摘要：Objective: The main active ingredients in Cephalotaxus hainanensis include cephalotaxine,harringtonine,homoharringtonine. This study aimed to establish a UPLC method for determination of cephalotaxine,harringtonine and homoharringtonine and analyze their metabolic accumulation and distribution in different tissues in C. hainanensis. Method: The method was performed on Agilent 1290Ⅱ UPLC platform. The suitable conditions of UPLC were Agilent Infinitylab EC-C18 column (2.1 mm×100 mm,2.7 μm,Agilent Infinitylab),methanol and 20 mmol·L-1 ammonium carbonate aqueous solution as mobile phase,gradient elution at 0.8 mL·min-1,detective wavelength at 291 nm,injection volume of 5 μL,and column temperature at 30℃. Result: Under the above chromatographic conditions,the chromatogram baseline was stable, and each target compound had a good peak shape and resolution. The results showed a good linear relationship between content and peak area in all three C.hainanensis alkaloids. The methodological experiment showed that the instrument precision,method reproducibility and recovery rate all conform to the requirements of quantitative determination. Conclusion: This determination method has a good precision,stability and repeatability,with the advantages of shorter determination time,simpler operation and more accurate results. All of these three alkaloids could be detected in 12 min. It can be used to determine the contents of cephalotaxine,harringtonine and homoharringtonine in C. hainanensis,and establish a foundation for further study of metabolic accumulation in C. hainanensis.关键词：UPLC;Cephalotaxus hainanensis;cephalotaxine;harringtonine;homoharringtonine6|6|0更新时间：2024-01-04
- 摘要：Objective: To study the chemical constituents from the ethyl acetate extract of Pteris vittata. Method: The whole plant of P. vittata was powdered and extracted with 95% EtOH for 3 times under reflux. After removal of the solvent,the 95% EtOH residue was suspended in water and successively extracted with petroleum ether,EtOAc and n-BuOH to obtain petroleum ether,EtOAc and n-BuOH extract respectively. The EtOAc extract was isolated and purified by silica gel,Sephadex LH-20,ODS columns and recrystallization,and the structures of their monomeric compounds were identified on the basis of physicochemical properties,NMR spectra,mass spectrum analysis and related literature. Result: Twelve compounds were isolated from the ethyl acetate extract and identified as apigenin(1),luteolin(2),luteolin-7,3',4'-trimethyl ether(3),chrysoeriol(4),7-hydroxy-8-methoxycoumarin(5),apigenin-7-O-β-D-glucuronide(6),luteolin-7-O-β-D-glucopyranoside(7),chrysoeriol-7-O-β-D-glucopyranoside(8),astragalin(9), p-hydroxybenzoic acid(10), β-sitosterol(11) and β-daucosterol(12). Conclusion: Compounds 2-5 and 8-12 were firstly isolated from P. vittata,providing reference for its clinical application and pharmacological studies.关键词：Pteris vittata;flavonoids;luteolin-7,3',4'-trimethyl ether;3'-methoxyluteolin5|6|2更新时间：2024-01-04
- 摘要：Objective: To optimize the synthesis process of octadecylamine-fluorescein isothiocynate (ODA-FITC),and evaluate its application. Method: ODA-FITC was synthesized by coupling reaction with ODA and FITC as raw materials,and the reaction process was monitored by thin layer chromatography. Its structure was verified by nuclear magnetic resonance spectroscopy,and the optimum synthesis process was obtained with the solvent,time,temperature and feed ratio as the investigation factors and with yield as the indicator. ODA-FITC was then used as a fluorescence marker for nanostructured lipid carriers(NLC) by solvent diffusion method. The ligated intestinal circulation model was established to observe the absorption and distribution of NLC in small intestine and evaluate its application. Result: The optimum reaction conditions were as follows:the molar ratio of ODA to FITC was 2:1,the solution was anhydrous ethanol,after reaction for 48 h at 60℃. Under such conditions,the yield was high,reaching (72.0±3.9)%,and the absorption and distribution of its marked nanoparticles in intestinal villus can be accurately evaluated. Conclusion: The synthesis method has complete reaction and high yield,with simple operation and high repeatability. Synthetic ODA-FITC can be used to intuitively evaluate the in vitro and in vivo conditions of nanoparticles.关键词：fluorescein isothiocynate;ODA-FITC;TLC;absorption by intestinal microvillus4|6|0更新时间：2024-01-04
- 摘要：Objective: To investigate the chemical constituents in Bushen Qiangshen pills (BQP) by ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF-MS). Method: The UPLC separation was performed on Agilent SB-C18 reversed-phase column (4.6 mm×100 mm,2.7 μm) with acetonitrile-0.1% formic acid solution as mobile phase for gradient elution. The flow rate was 0.5 mL·min-1,and the column temperature was 40℃. Data were collected via positive and negative ion mode using ESI ion source. First of all, the qualitative analysis was conducted for the components with reference substances, and the major components without reference substances were identified according to the relative molecule weight and fragment information by UPLC-Q-TOF-MS as well as comparison with literature data. Result: Fifty compounds from BQP were identified,including 19 phenolic acids,25 flavonoids,4 olefins,1 phenylethanolate and 1 alkaloid. 12 components (protocatechuic acid,salidroside,protocatechuic aldehyde,chlorogenic acid,hyperin,isoquercitrin,specnuezhenide,epimedin C,icariin,kaempferide, baohuosideⅠ and astragalin) among them were qualitatively analyzed by comparing with reference substances. Conclusion: UPLC-Q-TOF-MS is convenient and rapid to analyze the chemical constituents in BQP, providing a reference for the study on material foundation of efficacy,quality control and clinical application of BQP.关键词：Bushen Qiangshen pills;UPLC-Q-TOF-MS;phenolic acid;flavonoids7|7|5更新时间：2024-01-04
- 摘要：Objective: To investigate the relationship between the photosynthetic response characteristics, daily variation of photosynthetic parameters and environmental factors in leaves of Fagopyrum dibotrys. Method: The daily variation of photosynthetic parameters and light response curves of leaves of F. dibotrys were measured by portable photosynthesis apparatus. Result: Both diurnal variation of net photosynthetic rate(Pn) and the diurnal variation of stomatal conductance(Gs) in leaves of F.dibotrys showed a double peak curve,with an obvious midday depression phenomenon. Their net photosynthetic rate was 19.13 and 16.54 μmol·m-2·s-1 respectively,and bottom was at 14:00 with a net photosynthetic rate of 15.04 μmol·m-2·s-1,about 78.62% of the maximum peak value,showing a phenomenon of midday depression of photosynthesis. The net photosynthetic rate was positively correlated with the main influencing factors including photosynthetically active radiation (PAR),transpiration rate(Tr),stomatal conductance,and negatively correlated with atmospheric CO2 concentration (Ca) and intercellular CO2 concentration(Ci). Conclusion: F. dibotrys is a stomatal limiting plant,and Pn has obvious midday depression of photosynthesis phenomenon. Photosynthetic active radiation,transpiration rate,stomatal conductance and atmospheric temperature are the main influencing factors for Pn in leaves of F. dibotrys.关键词：Fagopyrum dibotrys;net photosynthetic rate;daily variation;environmental factors;clearning awayheat and toxic material6|6|0更新时间：2024-01-04
- 摘要：Objective: To establish a high performance liquid chromatography-mass spectrometry(HPLC-MS) method for simultaneously determining eight components in Xiaoer Anshen Bunao granule. Method: Agilent TC-C18(4.6 mm×250 mm,5 μm) column was adopted with acetonitrile-10 mol·L-1 ammonium acetate solution as the mobile phase for gradient elution,and the injection volume was 10 μL.Electrospray ionization (ESI) was used in both positive and negative modes,and the selected ion monitoring(SIM) were used for scanning. Result: Liquiritin,calycosin-7-O-β-D-glucoside, ammonium glycyrrhizinate,naringin,3,6'-disinapoyl sucrose,hesperidin,neohesperidin,astragaloside Ⅳ showed a good linearity in the ranges of 0.019 5-2.5 mg·L-1(r=0.999 1),0.078 1-5 mg·L-1(r=0.999 2),0.078 1-5 mg·L-1(r=0. 999 8),0.390 6-50 mg·L-1(r=0.999 9),0.019 5-2.5 mg·L-1(r=0.999 2),0.078 1-10 mg·L-1(r=0.999 0),0.312 5-20 mg·L-1(r=0.999 4),0.019 5-1.25 mg·L-1(r=0.999 0),respectively. Relative standard deviations (RSDs) of precision,stability and reproducibility were all less than 2.0%. The recoveries for the eight analytes were between 94.67% and 97.79%,with RSD less than 2.0%. The contents of liquiritin,calycosin-7-O-β-D-glucoside,ammonium glycyrrhizinate,naringin,3,6'-disinapoyl sucrose,hesperidin,neohesperidin,and astragaloside Ⅳ were 187.5-188.1,330.0-331.0,312.1-319.5,1 509.0-1 510.2,172.2-173.0,180.0-181.0,639.0-639.8,and 37.5-38.2 μg·g-1,respectively. Conclusion: This method is simple,accurate,sensitive and repeatable,which can be used for identification,quality evaluation of Xiaoer Anshen Bunao granule.关键词：Xiaoer Anshen Buano granule;liquiritin;calycosin-7-O-β-D-glucoside;ammonium glycyrrhizinate;naringin;3,6'-disinapoyl sucrose;hesperidin;neohesperidin;astragaloside Ⅳ6|6|3更新时间：2024-01-04
- 摘要：Objective: To establishultra high performance liquid chromatography (UPLC) method, analyze the contents of flavonoids and phenols ingredients in the different parts of Schisandra chinensis(SC),and evaluate their in vitro antioxidant capacities. Method: Analysis was performed on an ACQUITY UPLC BEH C18(2.1 mm×5.0 mm,1.7 μm) column with 0.01% phosphoric acid-acetonitrile as the mobile phase for gradient elution. The column temperature was maintained at 30℃. The detection wavelength of flavonoids was set at 360 nm,at the flow rate of 0.35 mL·min-1;and the detection wavelength of phenols was set at 280 nm,at the flow rate of 0.30 mL·min-1. Their antioxidant activities were compared and evaluated by DPPH and ABTS methods. Result: The results showed that the contents of flavonoids and phenols in different parts of SC were significantly different. The content of total flavonoids in leaves was about 3.8 folds and 49.5 folds of that in stems and fruits,respectively. The content of total phenols in leaves was about 4.2 folds and 21.5 folds of that in stems and fruits,respectively. The antioxidant activities of leaves and stems were significantly higher than those in fruits. The scavenging capabilities on DPPH free radicals in leaves and stems were 8.3 folds and 4.3 folds of that in fruits,respectively. The total antioxidant capacities with ABTS method in leaves and stems were 2.9 folds and 1.3 folds of that in fruits,respectively. Conclusion: The contents of total flavonoids and total phenols were positively correlated with their antioxidant activities,demonstrating that the antioxidant activity of SC may be related to the contents of total flavonoids and total phenols. The method was simple,accurate and reproducible, which can be used for the quantification of flavonoids and phenols ingredients of SC, providing the basis for clinical reasonable application of different parts of SC.关键词：Schisandra chinensis;flavonoid;phenol;antioxidant activity6|6|9更新时间：2024-01-04
- 摘要：Objective: To analyse and identify the chemical components between old leaves and young leaves of Cyclocandra paliurus, and compare the category and contents of components between the old leaves and young leaves. Method: The main chemical components in 50% methanol extract from old leaves and young leaves of C. paliurus were analyzed and identified by ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF-MS/MS). The contents of chlorogenic acid,kaempferol,isoquercitrin,quercitrin and quercetin of C. paliurus were determined by high performance liquid chromatography on an Inter Sustain-C18(4.6 mm×250 mm,5 μm) with acetonitrile-0.1% formic acid as mobile phase for gradient elution at a flow rate of 1.0 mL·min-1, and the detection wavelength was set at 254 nm and the column temperature at 30℃.Then the results were analyzed by using principal component analysis. Result: We finally identified 30 compounds from old leaves and young leaves. There were 21 common components among the two types of leaves. 2 components were only in young leaves,while 7 components were only in old leaves. There were quantitative differences in the contents of five components between old leaves and young leaves. 15 samples were divided into two main groups by principal component analysis. One was old leaves and the other was young leaves. Conclusion: The main chemical components in old leaves and young leaves of C. paliurus were basically similar. Comparatively,the chemical componentsin the old leaves were more abundant and the contents of five components were higher than thosein young ones. The established method could be used to betteridentify the chemical components in the leaves of C. paliurus,and distinguish the old leaves and young leaves, providing a reference for its quality control based on leaves of C. paliurus.关键词：Cyclocandra paliurus;old leaves;young leaves;UPLC-Q-TOF-MS/MS;chemical components analysis;content determination6|7|2更新时间：2024-01-04
- 摘要：Objective: To study on the tissue distribution of five components from Xinshao formula in rats after administered intragastrically. Method: UPLC-MS/MS for the simultaneous determination of apigenin-7-O-glucronide,scutellarin,scutellarin aglycone,paeoniflorin and albiflorin in rat tissues was established.Rats were administered intragastrically Xinshao formula at a dose of 25 g·kg-1,and main tissues and organs were collected from rats at 20, 90 min and 12,24 h after administration.After pretreatment,the concentrations of apigenin-7-O-glucronide,scutellarin,scutellarin aglycone,paeoniflorin and albiflorin in each organ of each time point were determined. Result: After being administered Xinshao formula,five components could be widely distributed in tissues and organs of rats.For apigenin-7-O-glucronide,its concentration peaked in the lung and heart at 20 min with 48.2,59.8 μg·L-1; peaked in the brain and small intestine at 90 min with 138.7,292.7 μg·L-1;peaked in kidney and muscle at 12 h with 517.3,56.5 μg·L-1;peaked in the liver at 24 h with 352.9 μg·L-1.For scutellarin,its concentration peaked in the heart at 20 min with 38.3 μg·L-1;peaked in the lung,brain,small intestine and kidney at 90 min with 30.6,220.0,1 644.2,859.5 μg·L-1;peaked in the muscle at 12 h with 86.9 μg·L-1 and in the liver at 24 h with 344.8 μg·L-1.For scutellarin aglycone,its concentration peaked in the brain,small intestine and muscle at 90 min with 875.4,52 468.0,5 114.7 μg·L-1;peaked in lung,heart and kidney at 12 h with 609.7,1 718.6,1 736.5 μg·L-1 and peaked in the liver at 24 h with 1 366.5 μg·L-1.For paeoniflorin,its concentration reached peaks in the lung,liver,kidney and muscle at 20 min with 10 634.1,13 889.0,16 672.0,7 750.4 μg·L-1;and reached in the heart,small intestine and brain at 90 min with 6 851.1,870 563.9,7 474.0 μg·L-1.For albiflorin,its concentration peaked in the liver and kidney at 20 min with 15 249.7,56 817.0 μg·L-1;peaked in the lung,heart,brain,small intestine and muscle at 90 min with 4 211.5,4 425.3,4 631.3,631 871.5,5 673.6 μg·L-1. Conclusion: After the rats administered intragastrically Xinshao formula,its five components could be widely and quickly distributed in various tissues and organs;however,there are differences in the peak time and distribution of these five components.The five representative components have the highest distribution in the kidney and small intestine,followed by the liver and muscle tissues,but also in the brain.Moreover,apigenin-7-O-glucronide,scutellarin,scutellarin aglycone may accumulate in the rat liver when the time of drug administered is extended.关键词：Xinshao formula;scutellarin;apigenin-7-O-glucronide;paeoniflorin;tissue distribution7|6|1更新时间：2024-01-04
- 摘要：Objective: To study on the effect of Xiaoyaowan on the endogenous metabolites in the urine of rats with nonalcoholic fatty liver disease(NAFLD) and liver depression and spleen deficiency syndrome,and to explore the therapeutic mechanism of this compound. Method: SD rats were randomly divided into 3 groups,namely blank group,model group and Xiaoyaowan group(3.24 g·kg-1·d-1).The high-fat and high-fat diet combined with irregular diet,chronic restraint stress stimulation,and tail clipping method were employed to replicate the NAFLD model for 10 weeks.Xiaoyaowan was given for 4 weeks.The levels of serum alanine aminotransferase,total cholesterol and triglycerides in the hepatic tissue,5-hydroxytryptamine and norepinephrine in brain tissue of model rats were detected by enzyme-linked immunosorbent assay.The morphology of liver tissue was observed by hematoxylin-eosin(HE) staining.The nucleus magnetic data of model rat urine was collected by metabolomics and combined with multivariate statistical analysis to explore the therapeutic mechanism of Xiaoyaowan. Result: Xiaoyaowan could make acetic acid,creatinine,glycine and other nine markers in the urine of rats with NAFLD and syndrome of liver depression and spleen deficiency have varying degrees of callback.It mainly involved the regulation of metabolic pathways,such as energy metabolism,glucose metabolism,insulin resistance,etc. Conclusion: Xiaoyaowan may play a role in the treatment of NAFLD by regulating lipid metabolism,glucose and amino acid metabolism disorders.关键词：nuclear magnetic resonance hydrogen spectrum (1H-NMR);Xiaoyaowan;nonalcoholic fatty liver disease;urine;metabolomics;syndrome of liver depression and spleen deficiency;energy metabolism6|5|8更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of cinobufacini on the invasion and migration of human gastric adenocarcinoma MGC-803 cells and its molecular mechanisms. Method: The inhibitory effect of cinobufacini on the proliferation of human gastric cancer MGC-803 cells was detected by methye thiazolye telrazlium (MTT) assay. The effect of cinobufacini on the invasion and migration of MGC-803 cells was determined by transwell assay. The protein expressions of matrix metalloproteinases-2 (MMP-2), matrix metalloproteinases-9 (MMP-9), tissue inhibitors of metalloproteinases-1 (TIMP-1) and tissue inhibitors of metalloproteinases-2 (TIMP-2) were examined by Western blot. After treatment with different concentrations of cinobufacini, the mRNA expressions of MMP-2, MMP-9, TIMP-1 and TIMP-2 in MGC-803 cells were detected by Real-time PCR. Result: ① MTT assay showed that cinobufacini (10, 20, 40, 80, 160, 320 mg·L-1) significantly inhibited the proliferation of gastric cancer MGC-803 cells (P<0.01) in a dose and time-dependent manner. ② Transwell assay results showed that cinobufacini (10, 20, 40 mg·L-1) inhibited the invasion and migration of gastric adenocarcinoma MGC-803 cells to varying degrees (P<0.05). ③ Real-time PCR and Western blot results showed that cinobufacini (10, 20, 40 mg·L-1) could down-regulate the protein and mRNA expressions of MMP-2, MMP-9 and up-regulate protein and mRNA expressions of TIMP-1, TIMP-2. Conclusion: Cinobufacini can obviously inhibit the invasion and migration of gastric cancer MGC-803 cells. The underlying mechanism may be related to the up-regulation of the transcription of TIMP-1 and TIMP-2 and the down-regulation of the protein levels of MMP-2 and MMP-9.关键词：cinobufacini;gastric cancer;invasion;migration;matrix metalloproteinase;matrix metalloproteinase inhibitor7|6|3更新时间：2024-01-04
- 摘要：Objective: To explore the role and mechanism of Celastrus orbiculatus extract (COE) in inhibiting the metastasis of gastric cancer, in order to seek new targets in preventing and treating gastric cancer with traditional Chinese medicine and lay a theoretical and experimental foundation for the development of effective anti-gastric cancer drugs are very important. Method: According to the results of the previous two-dimensional electrophoresis experiment, the differentially expressed target proteins of COE (20,40,80 mg·L-1) in gastric cancer were screened out. Then the expression of Cofilin 1 (CFL1) in the gastric cancer cells after COE intervention was detected by Western blot. Finally, the molecular cloning technique was used to inhibit the expression of CFL1 in gastric cancer BGC-823 cells, then COE was used to detect whether it could affect the metastasis of gastric cancer cells. Result: CFL1 was stably overexpressed in gastric cancer cells based on previous experiments. Western blot results showed that COE inhibited the expression of CFL1(P<0.01). Various concentrations of COE (20,40,80 mg·L-1) inhibited the expression of CFL1 in BGC-823 cells. And the expression of CFL1 was significantly inhibited by small interfering RNA (siRNA)(P<0.01). The transwell experiments showed that COE markedly reduced the number of transmembrane cells in low expressed BGC823 cells, with statistically significant differences(P<0.01). Conclusion: COE could significantly inhibit the metastasis of gastric cancer BGC-823 cells, and its mechanism may be related to the inhibition of overexpression of CFL1.关键词：Celastrus orbiculatus extract;traditional Chinese medicine;gastric cancer;cofilin;metastasis5|6|2更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of Jinlong capsule(JLC) on the invasion and migration of human gastric cancer MGC-803 and BGC-823 cells and its specific mechanism. Method: Gastric cancer MGC-803 and BGC-823 cells were cultured in vitro and divided into JLC group (with concentrations of 0.1, 0.2, 0.4, 0.8 g·L-1), negative control group and 5-fluorouracil (5FU) as positive control group. Methye thiazolye telrazlium (MTT) was used to detect the inhibitory effect of Jinlong capsule on the proliferation inhibition rate of MGC-803 and BGC-823 cells, in order to calculate the 50%inhibitory concentration (IC50); transwell and wound healing assay were used to detect the invasion and migration abilities of BGC-823 cell and MGC-803 cells after treatment with JLC for 24 h; after 24 h, the migration of MGC-803 and BGC-823 cells changed; Western blot method was used to detect the expression levels of E-cadherin, matrix metalloproteinase (MMP)-2 and MMP-9 after Jinlong capsule treatment for 24 h. Result: JLC could obviously inhibit the proliferation of MGC-803 and BGC-823 cells in a time-dependent manner; compared with the control group, JLC could significantly affect the invasion and migration abilities of the two kinds of gastric cancer cells in a concentration-dependent manner (P<0.05); the scratch distance of Jinlong capsule group was significantly lower than that in control group in a concentration-dependent manner (P<0.05); compared with control group, JLC group could increase the expression of E-cadherin and reduce the expression levels of MMP-2, MMP-9 in MGC-803 and BGC-823 cells, with a dose-response relationship (P<0.05). Conclusion: JLC can inhibit the invasion and migration of human gastric cancer MGC-803 and BGC-823 cells, and its mechanism may be related to the up-regulation of the expression of E-cadherin and the inhibition of the expression levels of MMP-2, MMP-9.This study has confirmed part of mechanisms of JLC on the invasion and metastasis of gastric cancer MGC-803 and BGC-823 cells.关键词：Jinlong capsule;invasion;migration;matrix metalloproteinase-2(MMP-2);matrix metalloproteinase-9(MMP-9);epithelial-mesenchymal transition (EMT)6|6|4更新时间：2024-01-04
- 摘要：Cancer is one of the most deadly diseases in the world. Gastric cancer is one of the most commonly seen digestive system tumors at present. Both of its morbidity and mortality remain high. Most of the patients died of advanced gastric cancer, and lost the chance of radical surgical treatment, but the prognosis of non-operative treatment was poor. Basic research on gastric cancer is expected to find an effective method for the treatment of gastric cancer and prolong the survival time of patients. Survivin gene is the strongest-ever apoptosis suppressor gene. It belongs to the family of inhibitor of apoptosis protein (IAP). The expression of Survivin is absent in normal gastric mucosa, and the gastric cancer cell line is overexpressed. Survivin gene has a unique molecular structure and biological function, and plays an important role in inhibiting cell apoptosis, regulating cell cycle, and participating in the pilot process of angiopoiesis. A large number of studies have indicated the relationship between Survivin gene and gastric cancer, suggesting that it may be a new potential marker for diagnosis of gastric cancer, and a detection indicator for gastric cancer. Traditional Chinese medicine (TCM) has a certain regulatory effect on this gene during inducing apoptosis of gastric cancer cells, which is bound to bring new hope, and opened up a new path for the treatment of tumor. In this paper, the biological characteristics of Survivin and its current research progress in gene therapy and clinical application of gastric cancer were reviewed by reference to literatures at home and abroad, in order to summarize the effect of Survivin in the pathogenesis of gastric cancer and the research progress of TCM and Western medicine in diagnosis and treatment.关键词：Survivin;gastric cancer;treatment;traditional Chinese medicine6|6|3更新时间：2024-01-04
- 摘要：Objective: To study the mechanism of total saponins of Baihe Zhimu Tang (SBZ) in regulating 5-hydroxytryptamine(5-HT) function for treatment of major depression, and to explore the pharmacodynamic advantages of SBZ versus total saponins of Lilii Bulbus (SL) or total saponins of Anemarrhenae Rhizoma (SA). Method: The depression model rats were induced by using unpredictable mild stimulation combined with feeding alone, and were divided equally into five groups:the major depression model group (MDD), total saponins of SL group (100 mg·kg-1), total saponins of SA group (220 mg·kg-1), total saponins of SBZ group (240 mg·kg-1) and fluoxetine hydrochloride group (FLU, 10 mg·kg-1). Another normal group was also set up, n=11 in each group. The normal group and MDD group received the equal amount of 0.5% Arabic gum solution, and corresponding doses of drugs were given in other groups. The immobility time in forced swimming test and the levels of 5-HT in blood and cerebral tissues of experiment rats were measured. Immunofluorescence staining was used to detect the positive expressions of 5-HT1a in the cerebral cortex and hippocampus. Reverse transcription and polymerase chain reaction (Real-time PCR) was used to test the mRNA transcription levels of genes in hippocampus:5-HT synthesis gene tryptophan hydroxylase(1TPH1),Synaptic vesicular monoamine transporter18A1(SLC18A1), synaptic protein-25(SNAP25); the expression of RGS19 in blood and hypothalamus were also detected. The expression of 5-HT transporter protein (SERT) in hippocampus and hypothalamus was detected by Western blot. Result: There was a significant difference between model group and normal group (P<0.01). As compared with model group, SBZ significantly reduced the immobility time of depressed rats in forced swimming test(P<0.05), up-regulated 5-HT levels in blood and brain (P<0.05) and the positive expression of 5-HT1a in cerebral cortex and hippocampus(P<0.05, P<0.01). According to the results of SBZ significantly increased the mRNA expression of TPH1, SLC18A1 and SNAP25 in hippocampal, and up-regulated the mRNA expressions of RGS19 in blood and hypothalamus(P<0.05,P<0.01); SBZ reduced the expression of SERT protein in hippocampus and hypothalamus(P<0.01), thereby increased the central content of 5-HT. Conclusion: SBZ can activate 5-HT synthesis gene TPH1 expression, down-regulate SERT expression, inhibit reuptake, increase the content of 5-HT in central tissues. SBZ could also up-regulate the expression of 5-HT1a receptor and its downstream gene RGS19 to regulate receptor function and signal transduction; improve the neurotransmitters transmission ability by up-regulating the expression of SLC18A1 and SNAP25, and finally achieve the anti-depression function. Results showed that SBZ had significant pharmacodynamic advantages in antidepressant efficacy as compared to SL and SA.关键词：total saponins of Baihe Zhimutang;antidepressant efficacy;5-hydroxytryptamine (5-HT);mechanism;pharmacodynamic advantages7|6|8更新时间：2024-01-04
- 摘要：Objective: To study the protective effect of essential oil of Alpinia zerumber fructus(EOAZF) on the inflammatory injury of human aortic endothelial cells (HAECs) induced by lipopolysaccharide (LPS). Method: HAECs were cultured in vitro. HAECs were pre-treated with different doses of EOAZF (0.05,0.1 mg·L-1) and aspirin (0.5 mmol·L-1) for 1 h, then incubated with lipopolysaccharide(LPS) (5 mg·L-1) for 12 h. The effect of EOAZF on the cell viability of HAECs induced by LPS was detected by methyl thiazolyl tetrazolium (MTT) assay, hematoxylin eosin(HE) staining was used to observe the cell morphology, and wound healing assay was used to analyze the ability of HAECs wound healing ability. Western blot was used to detect the protein expressions of vascular cell adhesion molecule-1 (VCAM-1), cysteine aspartase-3(Caspase-3), Toll like receptor 4 (TLR4), nuclear factor kappa B inhibitory protein kinase (IKK) and phosphorylated nuclear factor kappa inhibitory protein kinase (p-IKK), and real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect the mRNA expressions of TLR4 and VCAM-1.The nuclear translocation of nuclear factor kappa-B p65(NF-κB p65) was observed by immunofluorescence. Result: Compared with control group, LPS induced HAECs morphological damage, decreased cell viability and wound healing ability, and increased the expressions of VCAM-1, Caspase-3, TLR4, IKK and p-IKK. Immunofluorescence results showed that LPS induced nuclear translocation of NF-κB p65. Compared with model group, the pre-treatment of EOAZF improved the morphological damage of HAECs induced by LPS, increased cell viability and wound healing ability, inhibited the expressions of VCAM-1, Caspase-3, TLR4, IKK and p-IKK, as well as significantly inhibited the nuclear transposition of NF-κB p65. Conclusion: EOAZF has the protective effect on the inflammatory injury of HAECs induced by LPS, and its mechanism may be related to the regulation of TLR4/IKK/NF-κB p65 signaling.关键词：essential oil of Alpinia zerumber fructus;lipopolysaccharide;aspirin;endothelial cell injury;Toll like receptor 4;nuclear factor-kappa B4|6|4更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of resveratrol in delaying senescence of human cardiac microvascular endothelial cells (HCMEC),and to analyze its mechanism of action from the perspective of cytoskeleton. Method: Heat shock protein 27(HSP27) shRNA cell lines were established, and the cells were divided into 5 groups, the eighth generation replicative senescence model group (senescence group), resveratrol group (10 μmol·L-1), mock group, HSP27shRNA group, HSP27shRNA+resveratrol group (10 μmol·L-1). Cells senescence was detected by using β-galactosidase staining; cell counting kit-8 (CCK-8) was used to detect cell proliferation; laser confocal microscopy was used to observe the morphological changes of fibrous actin (F-actin) and globular actin (G-actin). Western blot was used to detect the protein expression of F-actin. Result: As compared with the senescence group, resveratrol could decrease the proportion of β-galactosidase stained (P<0.01), promote cell proliferation(P<0.01), maintain F-actin distribution in the cell periphery and G-actin distribution in the cell center, and reduce the protein expression(P<0.01). After silencing of heat shock protein 27 (HSP27), as compared with mock group, HSP27shRNA group and HSP27shRNA+resveratrol group could increase the proportion of β-galactosidase stained(P<0.01), decrease cell proliferation(P<0.01); F-actin around the cell periphery became irregular, sputtered and dissipated gradually, with unclear cell shape; G-actin showed blurred edges and stretched outside in cotton-like method, with staining dispersion in cell central region and irregular shape. The mean optical density value of F/G-actin was decreased(P<0.01), and the protein expression was also decreased(P<0.01). Conclusion: Resveratrol could delay endothelial senescence, promote cell proliferation, and the mechanism may be associated with down-regulation of the F-actin expression by HSP27.关键词：resveratrol;endothelial senescence;heat shock protein 27(HSP27) shRNA cell limes;F-actin6|6|5更新时间：2024-01-04
- 摘要：Objective: To investigate the effect and mechanism of psoralen on melanin content and tyrosinase activity in A375 cells. Method: Cells were inoculated in 6-well plates at a cell density of 2×105 cells and then divided into blank group, estradiol group, psoralen group, psoralen+estrogen receptor(ER) receptor blocker group (ICI182780), with the concentrations of 1×10-3,1,1 μmol·L-1, respectively. The melanin content and tyrosinase activity were detected by NaOH lysis method and dopaquinone oxidation method; Western blot and real-time fluorescent quantitative PCR assays were used to determine key protein kinases p38, c-Jun N-terminal kinase(JNK), extracellular signal-regulated kinase 2 (ERK2) and ocular hypotrophy-associated transcription factors (MITF) in the ER/mitogen-activated protein kinase(MAPK) pathway. The protein contents and corresponding mRNA expressions of tyrosinase (TYR), tyrosinase-associated protein-1 (TRP-1), and tyrosinase-associated protein-2 (TRP-2) were measured by dopa oxidation. Result: Compared with the blank group, the psoralen group had a significant inhibitory effect on melanin synthesis and TYR activity in A375 cells(P<0.01), with a significant inhibitory effect on the expression of estrogen receptor β(ERβ) protein in A375 cells (P<0.05), p38 MAPK, ERK and JNK protein expressions (P<0.01), protein expressions of MITF, TYR, TRP-1, and TRP-2 (P<0.01), and ERK2, p38 MAPK, MITF and TRP-1 mRNA expressions (P<0.01); compared with psoralen group, ICI182780 reversed the protein expressions of ERβ, ERK, MITF, TYR, TRP-1 (P<0.01) and the protein expressions of p38, JNK, and TRP-2 (P<0.05); at the same time, it can significantly block psoralen on ERK2, p38, MITF, TRP-1 mRNA expressions (P<0.01). Conclusion: Psoralen down-regulates the expressions of MITF and TYR, TRP-1, and TRP-2 through ER/MAPK signaling pathway, so as to reduce melanin synthesis.关键词：A375 cells;Psoralen;mitogen-activated protein kinase (MAPK) signaling pathway;melanin;estrogen receptor (ER) receptor blockers;phytoestrogen5|6|1更新时间：2024-01-04
- 摘要：Objective: To observe the effects of differences combined administrations of Gentianae Macrophyllae Radix (GMR) on serum rheumatoid factor (RF), C-reactive protein (CRP) and ankle nuclear factor kappa B (NF-κB) in rheumatoid arthritis (RA) model rats with wind-cold-dampness arthralgia. Method: Eighty healthy SD rats were randomly divided into blank control group, collage Ⅱ model group, wind-cold-dampness syndrome model group, positive control group, single-taste GMR group, GMR-Clematidis Radix et Rhizoma(CRR) group (GC group), GMR-Taxilli Herba group (GT group), GMR-Stephaniae Tetrandrae Radix group (GS group), with 10 rats in each group. Wind-cold-dampness Arthralgia model rats was induced in each group except for the blank group; collage Ⅱ model group was injected with collage Ⅱ and exposed to a wind-cold-dampness environment to prepare the wind-cold-dampness RA model of rats. After the establishment of the model, blank control group, collage Ⅱ model group and wind-cold-dampness syndrome model group were given normal saline, and the corresponding liquid medicines were given to each administration group. In the experiment, the thickness of the left posterior metatarsal of rats was measured every 3 days, and the swelling degree of the metatarsal was calculated. The arthritis index (AI) was evaluated on the 38th day of the experiment. The serum RF and CRP contents of rats were detected by enzyme linked immunosorbent assay (ELISA). The expression of NF-κB in ankle joint was detected by Western blot. The NF-κB mRNA expression in ankle joint was detected by Real-time fluorescence quantitative PCR. Result: Compared with the blank group, the swelling degree, AI score of foot, serum RF and CRP levels, of NF-κB protein and mRNA expressions in the ankle joint of collage Ⅱ model group and wind-cold-dampness syndrome model group were significantly increased (P<0.01). Compared with the wind-cold-dampness syndrome model group, the swelling degree, AI score of foot, serum RF and CRP levels, NF-κB protein and mRNA expressions in the ankle joint of the traditional Chinese medicine group were decreased(P<0.05,P<0.01), especially in GC group. Conclusion: The therapeutic effect of mild and warm traditional Chinese medicine (TCM) is better than that of the combined administration of mild and cold TCM or mild TCM drugs for rheumatoid arthritis with wind-cold-dampness arthralgia. The experimental results basically conform to the principle of ‘treating cold diseases with hot medicine’ in TCM.关键词：Gentianae Macrophyllae Radix;wind-cold-dampness arthralgia;rheumatoid arthritis;rheumatoid factor;C-reactive protein;nuclear factor kappa B7|5|10更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of sanguinarine on pancreatic cancer cell apoptosis and its signal pathway regulation mechanism. Method: After Panc02 cells were treated with different concentrations of sanguinarine(2,4,6 μmol·L-1) for different periods of time(24,48,72 h), the inhibition of cell growth was determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The effect of sanguinarine on cell apoptosis was determined by phospholipid binding protein V/propidium iodide(Annexin V-FITC/PI) double staining flow cytometer assay. The changes in mitochondrial membrane potential were examined by cyanine dye (JC-1) staining assay. The expressions of protein B-lymphoblast-2 (Bcl-2),Bcl-2 related X protein(Bax),total protein kinase B (Akt),phosphorylated protein kinase B (p-Akt),total phosphatidylinositol 3 kinase (PI3K),phosphorylated phosphatidylinositol 3-kinase(p-PI3K) were measured by Western blot analysis. Result: Compared with the blank group, the growth inhibition rate of Panc02 cells increased gradually with the rise of the drug concentration, after being treated with different concentrations of sanguinarine for the same period of time (P<0.05). Compared with the blank group, the inhibition rate of cell growth increased gradually with the rise of time, after being treated with the same concentration for different periods of time (P<0.05). Compared with the blank group, the apoptosis rate was not obviously increased at the dose of the 2 μmol·L-1 sanguinarine. Compared with the blank group, the apoptotic rate was increased at the dose of the 4,6 μmol·L-1 sanguinarine (P<0.05). Compared with the blank group, there was no change in the red and green fluorescence at the dose of 2 μmol·L-1, but a decrease in red fluorescence, an increase in green fluorescence and a reduction in Panc02 mitochondrial membrane potential at the dose of 4,6 μmol·L-1 sanguinarine. Compared with the blank group, the expressions of Bax, Bcl-2, p-PI3K, p-Akt, Akt and PI3K were not changed at the dose of the 2 μmol·L-1 sanguinarine. The expression of Bax was increased, while the expressions of Bcl-2 and PI3K/Akt signaling pathway in key proteins p-PI3K, p-Akt were decreased at the dose of 6 μmol·L-1 sanguinarine (P<0.05). Conclusion: Sanguinarine can effectively induce the apoptosis of pancreatic cancer Panc02 cells through the mitochondrial apoptotic pathway by inhibiting the PI3K/Akt signaling pathway.关键词：pancreatic cancer;sanguinarine;cell apoptosis;Panc02 cells;phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway7|6|5更新时间：2024-01-04
- 摘要：Objective: To explore the material basis of couplet medicines of Ephedrae Herba and Armeniacae Semen Amarum in reducing airway damage in rats,and to explore its mechanism of action. Method: Male Sprague-Dawley(SD) rats were adaptively reared for 3 days.After screening,they were randomly divided into 9 groups[normal group,model group,dexamethasone group,and Ephedrae Herba-Armeniacae Semen Amarum with different ratio groups(N1-N6 groups)].At 9:00 am,the corresponding drugs were intragastrically administered.Immediately after 1 hour of intragastric administration,2% acetylcholine chloride+0.4% histamine phosphate was used to induce asthma and the incubation period was recorded for 7 days.Immediately after the last asthmatic anesthesia,the main trachea was taken out,some of the trachea were fixed with 10% formaldehyde,and hematoxylin-eosin(HE) staining was used to observe the pathological features of each group.Another part of the tracheal homogenate was treated by enzyme-linked immunosorbent assay for the concentration of epidermal growth factor(EGF),inducible nitric oxide synthase(iNOS) and endothelin-1(ET-1).After the adaptive feeding of 6 rats in the same batch,the jejunum and ileum intestine were separated to prepare the drug-containing intestinal absorption liquid.Ultra-high performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS) was used to analyze the major differential components and their content in the drug-containing intestinal absorption liquid of Ephedrae Herba-Armeniacae Semen Amarum.Multiple linear regression analysis was used to analyze the correlation between the effect indicators and the main differential components. Result: The differential components in the intestinal absorption liquid of N1-N6 groups were ephedrine,pseudoephedrine,methylephedrine and amygdalin;the effect indicators were asthmatic latency,ET-1,iNOS,EGF mass concentrations and total score of airway pathology. Conclusion: The main material basis of Ephedrae Herba-Armeniacae Semen Amarum for prolonging the incubation period and repairing airway damage are ephedrine,pseudoephedrine,methylephedrine and amygdalin.The main active ingredient that reduces iNOS and ET-1 levels,inhibits EGF production,and maintains lower levels of NO/ET is methylephedrine.It indicates that Ephedrae Herba was the main medicinal taste,and Armeniacae Semen Amarum assists repairing airway damage.The N3 group has the best therapeutic effect.关键词：Ephedrae Herba-Armeniacae Semen Amarum;couplet medicines;incubation period for asthma;methylephedrine;inducible nitric oxide synthase;endothelin-1;Epidermal Growth Factor6|7|7更新时间：2024-01-04
- 摘要：Objective: To explore the potential mechanism of Gegen Qinlian Tang(GQT) in the treatment of type 2 diabetes mellitus (T2DM) using network pharmacology. Method: Four chemical components of GQT and target genes of T2DM were collected in Computation Platform for Integrative Pharmacology of Traditional Chinese Medicine (TCMIP). The drug targets were screened out according to the Similarity of Two-Dimensional structures. The online Cytoscape technology in TCMIP was used to build the core target network and "herbs-ingredient-target-pathway" network. Gene Ontology(GO) gene function combined with Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment module was used to annotate and analyze the gene function and signaling pathway of the core target. Result: Based on the TCMIP, 234 chemical components of GQT and 175 core targets were collected. Multi-dimensional network analysis showed that 68 active components had strong interactions with protein kinase C-δ(PRKCD), glucokinase (GCK), cytochrome C oxidase subunit (COX7C/COX5A/COX5B), ATP synthase F1 subunit (ATP5F1A/ATP5F1C), insulin receptor (INSRS) and other 22 major proteins; the biological pathways mainly involved in the endocrine system,cell growth and death, FoxO signaling pathway, adipocytokine signaling pathway, insulin signaling pathway and so on. Conclusion: The findings preliminarily reveal the effective mechanism of GQT in treating T2DM, which is related to anti-inflammation, anti-oxidation stress, anti-insulin β cell injury, and improvement of glycolipid metabolism and complications.关键词：network pharmacology;Gegen Qinlian Tang;type 2 diabetes mellitus;effective mechanisms11|6|15更新时间：2024-01-04
- 摘要：Objective: To explore the intervention mechanism and safety of compound Qima capsule in the treatment of carotid atherosclerosis plaques by observing its clinical efficacy. Method: Eighty patients with carotid atherosclerosis plaques were randomly divided into control group and treatment group (40 cases in each group). Patients in control group were treated with atorvastatin calcium tablets for 6 months. Patients in treatment group were additionally treated with compound Qima capsule for 6 months. The changes in the indicators were compared and the efficacy was assessed in both groups. Before and after treatment, thickness of carotid intima of the carotidartery (IMT) and atherosclerotic plaque area were detected by color Doppler ultrasound; levels of triglycerides (TG), total cholesterol (TC), high density lipoprotein-C (HDL-C) and low density lipoprotein-C (LDL-C) were detected; level of nitric oxide(NO), endothelin-1(ET-1) and 6-ketone prostaglandin F1 α (6-Reto-PGF1α) were detected. Levels of interleukin-6 (IL-6), tumor necrosis factor-α(TNF-α), and matrix metalloproteinases-9 (MMP-9) were also detected.In addition, adverse drug reactions were compared between two groups. Result: After treatment,the total effective rate in treatment group was significantly higher than that in control group (P<0.05). By comparison with control group, the blood lipid levels and inflammatory factors in treatment group were significantly reduced (P<0.05), vascular endothelial function was significantly improved (P<0.05), bilateral carotid plaque area and carotid intima-media thickness were significantly reduced after treatment (P<0.05). There were no adverse drug reactions in both groups during the treatment. Conclusion: Compound Qima capsule can significantly decrease the bilateral carotid plaque area and carotid intima-media thickness, and improve the symptoms of carotid atherosclerosis with high safety for patients with carotid atherosclerosis plaques, and the mechanism may be related to regulating lipid metabolism, improving the endothelial function and anti-inflammatory response.关键词：compound Qima capsule;atorvastatin calcium;carotid atherosclerosis;plaque;safety evaluation6|6|0更新时间：2024-01-04
- 摘要：Objective: To observe the effect of Zishen Jianpi Huayu prescription on the coagulation function of patients with recurrent spontaneous abortion with spleen and kidney deficiency and blood stasis resistance winding, in order to explore its mechanism. Method: A total of 115 eligible patients were randomly divided into group A, B and C, with 35 cases in each group. Zishen Jianpi Huayu prescription, aspirin tablets and low molecular weight heparin calcium were provided respectively. The scores of the traditional Chinese medicine (TCM) syndrome differentiation spleen and kidney deficiency coalescing blood stasis (TCM syndromes) and blood coagulation function[part of the original activators fibrinolytic enzyme inhibitor-1 (PAI-1), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen (FIB), tissue plasminogen activator (t-PA), prothrombin time (PT), D-dimer (D-D), tissue factor (TF), fibrin peptide A (FPA), lupus anticoagulant (LA), activated protein S (PS), activated protein C (PC), anti cardiolipin antibody (ACA), platelet aggregation function (PAF), fibrinogen (Fig), prothrombin-anti fibrinolytic prothrombin complex (PAP), prothrombin fragment (F1+2), antithrombin (AT), thrombin-antithrombin time (TAT)]before and after treatment were observed. The effective rates and adverse reactions in each group were compared. Result: The total effective rate of group A was 84.4%, which was higher than 58.1% of group B and 60.6% of group C (P<0.05). Compared with group B, TCM syndrome score and coagulation function of group A were significantly improved(P<0.05). To compare with group C, TCM syndrome score and coagulation function of group A were significantly improved (P<0.05). The total adverse reaction rate of group A was 3.1%, which was lower than 38.7% of group B and 30.3% of group C (P<0.05). Conclusion: Zishen Jianpi Huayu prescription can significantly alliviate the clinical symptoms and improve the coagulation function of the patients with recurrent miscarriage with spleen and kidney deficiency and blood stasis, with a low incidence of inactivity.关键词：Zishen Jianpi Huayu prescription;recurrent spontaneous abqrtion;coagulation function;spleen and kidney deficiency;blood stasis resistance winding7|6|5更新时间：2024-01-04
- 摘要：Objective: To observe the effect of maintenance therapy of Aikangning mixture on progression free survival (FFS), quality of life and immunity of patients after chemotherapy for non-small cell lung cancer (NSCLC). Method: One hundred and fifteen NSCLC patients at a stable period after chemotherapy were randomly divided into control group (58 cases) and observation group (57 cases) by random number table. Patients in control group got Shenyi capsules, 2 grains/time, 2 times/days. Patients in observation group got Aikangning mixture, 30 mL/time, 3 times/days. The course of follow-up was once every 1-2 weeks, and the treatment was continued to the tumor progression. Cycle of maintaining the treatment was recorded. CT detection every 4 weeks to evaluate the size of tumor, and PFS (time from patients joining to the team to death or progression of disease) was recorded in both groups; karnofsky performance scale (KPS) was scored every 4 weeks. Traditional Chinese medicine (TCM) syndromes were graded for every 4 weeks. Levels of T lymphocyte subgroup (CD3+, CD4+, CD8+ and CD4+/CD8+), natural killer cells (NK), vascular endothelial growth factor (VEGF), carcinoembryonic antigen (CEA), sugar antigen 125 (CA125), and cytokeratin19 fragment21-1 (CYFRA21-1) were detected before and after treatment. Result: The average maintenance therapy in observation group was (20.23±5.94) weeks, longer than (15.75±4.81) weeks in control group (P<0.01). PFS was (25.37±7.43) weeks in control group, shorter than (37.41±9.15) weeks in observation group. The median PFS in observation group was 12 weeks later than control group. At 12th and 16th weeks, the effects for solid tumor and KPS in observation group were superior to those in control group (P<0.05,P<0.01). Except for scores of nerve fatigue, weakness and shortness of breath, the other scores of observation group were all lower than those in control group (P<0.01). After treatment, the levels of CD4+ and CD4+/CD8+ in observation group were higher than those in control group, and CD8+ was lower than that in control group (P<0.05). Levels of VEGF, CEA, CA125 and CYFRA21-1 in serum were increased in both groups (P<0.01), but levels of VEGF, CEA, CA125 and CYFRA21-1 in observation group were lower than those in control group after treatment (P<0.01). Conclusion: Aikangning mixture for patients with non-small cell lung cancer after chemotherapy can extend progression free survival, stabilize the illness, relieve clinical symptoms and improve patients' quality of life and immune function of body, and can also inhibit expression of VEGF, CEA, CA125 and CYFRA21-1 in serum.关键词：non-small cell lung cancer;maintenance therapy;Aikangning mixture;quality of life;progression free survival;immune function6|6|4更新时间：2024-01-04
- 摘要：Objective: To discuss the clinical efficacy of addition and subtraction therapy of Qingzao Jiufei Tang to acute exacerbation of chronic bronchitis (wind dryness and lung injury) in plateau area and investigate its influence to inflammatory factors. Method: One hundred and forty patients were randomly divided into control group (70 cases) and observation group (70 cases) by random number table. Both groups received anti-infection treatment, dispelling phlegm and relieving cough. Moxifloxacin hydrochloride tablets for 7 days, 0.4 g/time, qd, ambroxol hydrochloride tablets for 14 days, 30 mg/time, bid. Patients in control group got Xingsu Zhike granules, 12 g/time, bid, taken after being infused in warm boiled water. Patients in observation group got addition and subtraction therapy of Qingzao Jiufei Tang, 1 dose/day. The treatment course was 14 days in both groups. Before and after treatment, scores of cough, visual analogue scale (VAS) of cough and scores of wind dryness and lung injury were graded. The disappearance time of cough, phlegm, dry nose and pharynx, rhonchi of lung were recorded. Both before and after treatment, the pulmonary function was detected, and levels of forced vital capacity (FVC), forced expiratory volume in one second (FEV1), percentage of FEV1 to expected value (FEV1%), tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), monocyte chemoattractant protein-1, endothelin (ET) and malondialdehyde (MDA) were detected. Result: The total effective rate for the disease in observation group was 93.65%, better than 81.67%in control group (χ2=4.122, P<0.05). The total clinical effective rate for traditional Chinese medicine(TCM) in observation group was 93.65%, better than 80%in control group (χ2=5.061, P<0.05). Scores of cough symptoms (day and night), VAS scores of cough and scores of wind dryness and lung injury in observation group were all lower than those in control group (P<0.01). FEV1, FEV1/FVC and FEV1% were improved than those before treatment in both groups (P<0.05), and the improvement was more obvious in observation group (P<0.05). The disappearance periods of cough, phlegm, dry nose and pharynx, rhonchi of lung in observation group were shorter than those in control group (P<0.05, P<0.01). And the serum levels of MCP-1, IL-8, TNF-α, ET and MDA were lower than those in control group (P<0.01). Conclusion: Based on the routine western medicine treatment for anti-infection, Qingzao Jiufei Tang can quickly control clinical symptoms, shorten course of disease, and ameliorate pulmonary function, with anti-inflammatory and anti-oxidative stress functions when it is used for chronic bronchitis (wind dryness and lung injury) at acute exacerbation in plateau area.关键词：chronic bronchitis;plateau area;acute exacerbation;wind dryness and lung injury;Qingzao Jiufei Tang;inflammatory factors;endothelin;malondialdehyde6|6|2更新时间：2024-01-04
- 摘要：In view of the current overall deficiencies and slow progress in the study of traditional Chinese medicine(TCM) syndrome models, related researches on superficies syndrome model were comprehensively analyzed based on the decades of research experience in superficies syndrome model as well as the latest advances in modern science and technology, so as to find a new breakthrough point in the establishment of the superficies syndrome model. Literature related to superficies syndrome model(or upper respiratory tract infection) was searched on CNKI, Pubmed and other databases,and was elaborated, analyzed and summarized in detail in accordance with the modeling method, showing the progress, shortage and possible breakthrough. Great progress has been made recently in many aspects such as modeling methods, prescription research and test, and biochemical characterization, but there are also many shortcomings such as poor clinical correlation, unsatisfactory correspondence between prescription and syndrome, inconsistent modeling indexes, vague criteria for evaluation of evidences. It is necessary to break through the original thought fetters and draw lessons from the progress on modern life science, and try to establish a multi-level and more elaborate system of pathological model for superficies syndrome. It is the core proposition to strengthen model studies by closely combining with the clinical research, fully absorb the progress of life science and other disciplines, and establish a more sophisticated and more detailed research system for superficies syndrome pathology model, all of which need to be solved urgently. This is helpful for the in-depth study of the superficies syndrome theory, prescription solution and its clinical practice, which can solve the urgent need of people's health in face of the increasingly serious outbreaks,and can also provide reference for researches on other syndrome models.关键词：superficies syndrome;syndrome model;cytokine;modeling method6|6|5更新时间：2024-01-04
- 摘要：The degree of renal interstitial fibrosis is closely related to the deterioration of renal function and the prognosis of chronic kidney disease. Renal fibrosis is a major pathophysiological change in almost all kinds of renal diseases developing to the end-stage renal failure. The formation causes of renal fibrosis include kidney trauma, infection, immune response, and damage of natural cells. The therapies of western medicine include bailing capsule, huangkui capsule, statins, angiotensin converting enzyme inhibitors (ACEI) and angiotensin Ⅱ receptor antagonist (ARB), gene therapy, cell factor and targeted drug therapy. Traditional Chinese medicine(TCM) believes that renal fibrosis is a process of decline in upright force, growth of evil trends, long-term struggle between evil trends and upright force, efficiency of evil trends and deficiency of upright force. Regarding asthenia in origin and sthenia in superficiality, sthenia in superficiality means deficiency of vital energy, and asthenia in origin means blood stasis. TCM treats renal fibrosis mainly by activating blood stasis and dissolving stasis, clearing heat and detoxicating, and invigorating spleen and kidney, which is a multi-link, multi-location and multi-target intervention therapy. The early stage of renal fibrosis features the spleen and kidney Qi deficiency and the deficiency of Qi and Yin; Codonopsis pilosula, Rehmannia, Chinese wolfberry and yam are mainly used to tonify Qi and nourish Yin, replenish spleen and tonify kidney. The advanced kidney disease features blood stasis and pathogenic toxin block meridian. Paeoniae Alba Radix, Angelicae Sinensis Radix, Persicae Semen, and Achyranthis Radix are mainly adopted for activating blood and dissolving stasis, clearing heat and detoxicating by Paeoniae Paeoniae Radix, peony skin, Smilax Glabra, and Rehmannia; at the same time, Ligustrum, Eclipta Alba, Radix Codonopsis, Astragali Radix are added to invigorate the kidney and spleen. This paper discusses the mechanism of renal fibrosis from the perspective of modern medicine and TCM, as well as experimental studies on improving renal fibrosis, delaying kidney failure with traditional Chinese and western medicine. It provides a sufficient theoretical basis for the clinical treatment of renal fibrosis.关键词：renal fibrosis;modern medicine;traditional Chinese medicine;treatment by traditional Chinese and western medicine;activating blood and dissolving stasis;clearing heat and detoxicating;invigorating spleen and kidney7|7|14更新时间：2024-01-04
- 摘要：Chlorogenic acid is a kind of styrene acrylic analogues synthesized through photosynthesis, with high content in many kinds of Chinese medicinal materials as the main pharmacological active ingredient. Due to its extensive pharmacological effect, it is widely used in areas such as food, medicine and chemical industry. The molecular structure contains ester bond and unsaturated double bond, and other unstable structures, easy to oxidation, and it will lose its activity under light and heating conditions, but it is stable in acidic environment. It has anticancer, hypolipidemic, antioxidant, hypoglycemic, ultraviolet and radiation resistant, antibacterial, immune regulation and other functions. With the progress of science and technology and the deepening of the research, more focus has been put on anti-tumor activity. Chlorogenic acid has anti-tumor effect, showing better prevention and treatment effect on lung cancer, liver cancer, breast cancer, nasopharyngeal carcinoma, colon cancer, cervical cancer, stomach cancer, oral cancer, leukemia, retinoblastoma, melanoma, skin cancer and other tumors with higher incidence. The mechanism of chlorogenic acid may be related to blocking cell growth cycle, inhibiting tumor cell proliferation, inducing tumor cell apoptosis and inhibiting tumor angiogenesis, inhibiting metastasis, inhibiting telomerase activity, immune regulation, reversing multi-drug resistance of tumor cells, and so on. By taking chlorogenic acid, antitumor, etc as keywords, we searched related papers in Pubmed, VIP, CNKI and other databases between January 1999 and January 2018, and more than 110 related documents were obtained, but only 51 of them were effective. We reviewed the anti-tumor function and mechanism in effective papers, hoping to provide the reference for the deep research of anti-tumor activity of Chlorogenic acid.关键词：chlorogenic acid;anti-tumor;mechanism11|7|11更新时间：2024-01-04
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