最新刊期
卷 24 ， 期 22 ， 2018
- 摘要：Objective:To investigate change law of saccharides in Rehmanniae Radix Praeparata with different processing times based on metabolomics. Method:Samples processed by one to nine cycles of repeated steaming and drying with traditional method were collected,which were derived with 1-phenyl-3-methyl-5-pyrazolone(PMP),then subjected to LC-MS analysis.The principal component analysis(PCA) and orthogonal partial least squares-discrimination analysis(OPLS-DA) were performed with SIMCA-P 13.0,then difference markers were obtained,meanwhile,which were identified by ion fragmentation and reference substance. Result:Six saccharides were identified by OPLS-DA,namely manninotriose,melibiose,mannose,glucose,galactose and arabinose.In which the relative peak areas of manninotriose and melibiose gradually reduced to 0.60 and 0.52 in ninth cycles,respectively;while arabinose had a increasing trend,the relative peak area was highest in ninth cycles and reached up to 4.74.Meanwhile,mannose firstly increased and then reduced,the relative peak area was 3.33 in fifth cycles and it was higher than other cycles;but glucose and galactose remained relatively stable.The score plot of PCA showed that Rehmanniae Radix Praeparata processed by 1 to 9 cycles gradually changed along the PC1 axis and saccharides were significant changed. Conclusion:The saccharides in Rehmanniae Radix Praeparata with different processing times are quite different.This paper can provide a reference for quality evaluation of Rehmanniae Radix Praeparata processed by nine cycles of repeated steaming and drying.关键词：metabolomics;principal component analysis;orthogonal partial least squares-discrimination analysis;saccharides;Rehmanniae Radix Praeparata;arabinose;1-phenyl-3-methyl-5-pyrazolone2|2|7更新时间：2024-09-23
- 摘要：Objective:To investigate the correlation between intrinsic quality and the appearance color of Salviae Miltiorrhizae Radix et Rhizoma. Method:The contents of effective ingredients(salvianolic acid B and tanshinones) in Salviae Miltiorrhizae Radix et Rhizoma were determined by HPLC.The extract content was determined by methods in the 2015 edition of Chinese Pharmacopoeia and the chromatic values of methanol solution of Salviae Miltiorrhizae Radix et Rhizoma powder were measured by colorimeter.On the basis of this,the three sets of data were fitted to find out the relationships. Result:The contents of effective ingredients were higer,the content of extract was higher,the value of a*(red and green weight value) was higher,and the color was redder,then the quality of Salviae Miltiorrhizae Radix et Rhizoma was better;the opposite is the same. Conclusion:A simple method,which is used to judge the quality of Salviae Miltiorrhizae Radix et Rhizoma,is established based on the correlation analysis of appearance and intrinsic quality,this method can be also used to verify scientificity of traditional identification experience of "redder is better".关键词：Salviae Miltiorrhizae Radix et Rhizoma;intrinsic quality;value of chromatism;correlation analysis;salvianolic acid B;tanshinones;extract2|2|3更新时间：2024-09-23
- 摘要：Objective:To study on the intestinal absorption of six alkaloids in Aconiti Lateralis Radix Praepareta(ALRP)-Glycyrrhizae Radix et Rhizoma(GRR) before and after their compatibility by in situ perfusion method in rats. Method:A rat intestinal perfusion model was used,the mass concentration of phenol red was measured by ultraviolet spectrophotometry,and the contents of aconitine,hypaconitine,mesaconitine,benzoylaconine,benzoylhypaconine,benzoylmesaconine in the perfusate were measured by LC-MS.Effect of three modes of compatibility on intestinal absorption of six alkaloids in ALRP decoction,ALRP-GRR combined decoction and ALRP-GRR separated decoction were investigated. Result:Compared with ALRP decoction,absorption rate constant(Ka) and absorption rate per unit time(A) of benzoylaconine in ALRP-GRR combined decoction and ALRP-GRR separated decoction showed a downward trend.Compared with ALRP-GRR separated decoction,Ka and A of benzoylmesaconine in ALRP-GRR combined decoction and ALRP decoction was decreased.Compared with ALRP decoction,Ka and A of hypaconitine in ALRP-GRR combined decoction showed a decreasing trend,Ka and A of hypaconitine in ALRP-GRR separated decoction showed a increasing trend.Compared with ALRP decoction,absorption of benzoylhypacoitine,aconitine and mesaconitine in ALRP-GRR combined decoction was in decline. Conclusion:Absorption of six alkaloids from ALRP in duodenum of rats decreases to some extent after combined with GRR.This study provides an experimental basis for the interpretation of the compatibility theory of "ALRP used with GRR can be absorbed slowly".关键词：Aconiti Lateralis Radix Praepareta;Glycyrrhizae Radix et Rhizoma;benzoylaconine;aconitine;hypaconitine;mesaconitine;in situ intestinal absorption;in situ intestinal perfusion model2|2|8更新时间：2024-09-23
- 摘要：Objective:To develop a saline water salting and drying technology for obtaining the medicinal materials of Angelicae Dahuricae Radix(ADR) in place of origin and avoiding rot and mildew. Method:The slightly wilted fresh ADR was tiled in a container,immersed in the saline water,pressed with stone on the surface,pickled for 1-2 d,discard the saline water,added almost equal amount of fresh saline water,pressed with stone on the surface again,sealed,pickled for a period of time,picked up,soaked and rinsed with water,put in a cool ventilated place to dry,and obtained the medicinal materials of ADR. Result:The medicinal materials of ADR processed by saline water salting and drying technology met all stipulation in the 2015 edition of Chinese Pharmacopoeia about ADR,and the content of nitrite in all samples was lower than 3.3 mg·kg-1(detection limit),which was in line with the limit index of nitrite in salted products. Conclusion:A new saline water salting and drying technology is developed to make medicinal materials of ADR in place of origin,and no metamorphism has been observed for 2 years later stored in simple warehouse.关键词：Angelicae Dahuricae Radix;saline water salting method;processing in production place;drying technology;storage;nitrite;salted products2|2|5更新时间：2024-09-23
- 摘要：Objective:To establish an aging year identification model of Citri Reticulatae Pericarpium by principle of chromaticity analysis on its exterior color. Method:Color of Citri Reticulatae Pericarpium samples in different aging years were objectively converted to three values by color colorimeter.Statistic methods,including non-parametric test,rank correlation test and discriminant analysis,were applied to analyze the correlation between aging year and L*(brightness value),a*(red and green component value) and b*(yellow and blue component value). Result:There were significant differences among three chromaticity values in different aging years;L*,a* and b* values were linearly related to aging year.An aging year identification model of Citri Reticulatae Pericarpium was established.The formula was aging year=-43.637L*+5.351a*+42.430b*+30.790. Conclusion:The new method provided by this paper for identification of aging year of Citri Reticulatae Pericarpium is objective and accurate.It can avoid the error caused by traditional empirical method in practical application.关键词：principle of chromaticity analysis;Citri Reticulatae Pericarpium;appearance color;aging year;non-parametric test;discriminant analysis;identification model4|2|6更新时间：2024-09-23
- 摘要：Objective:Based on the HPLC specific chromatograms and the contents of main chemical components,to discuss the change law of substance basis in Paeoniae Radix Alba decoction pieces before and after being processed. Method:RP-HPLC was employed with ZORBAX SB-C18 column(4.6 mm×250 mm,5 μm),a gradient mobile phase of acetonitrile-0.05% phosphoric acid aqueous solution was eluted,the column temperature was 30℃ and the flow rate was 1.0 mL·min-1,detection wavelength was set at 254 nm,the method for characterizing the characteristics of Paeoniae Radix Alba decoction pieces was established;at the same time,gallic acid and other 5 ingredients were simultaneously determined at 230 nm.The contents of 1,2,3,4,6-pentagalloylglucose and benzoic acid were simultaneously determined with mobile phase of acetonitrile-0.05% phosphoric acid aqueous solution(18:82). Result:Paeoniae Radix Alba and fired Paeoniae Radix Alba decoction pieces were marked with twelve characteristic peaks,and eight of them were assigned.After being processed,the contents of paeoniflorin and albiflorin decreased by 6% and 7%,respectively;the content of tannins increased significantly,the contents of gallic acid,1,2,3,4,6-pentagalloylglucose and benzoic acid increased by 28%,26% and 53%,respectively;while the contents of other components did not change significantly. Conclusion:According to the specific chromatograms and contents of main chemical components in this article,it can provide reference for formulation of quality evaluation criteria of Paeoniae Radix Alba and fired Paeoniae Radix Alba decoction pieces,which lays the foundation for further improving the quality evaluation system of them.关键词：Paeoniae Radix Alba;fired Paeoniae Radix Alba;decoction pieces;specific chromatograms;material basis;paeoniflorin;albiflorin;1,2,3,4,6-pentagalloylglucose3|2|1更新时间：2024-09-23
- 摘要：Objective:To investigate the effect of compatibility of Tripterygii Radix et Rhizoma and Paeoniae Radix Alba on the percutaneous absorption of triptolide and two alkaloids(wilforgine and wilforine),to investigate the mechanism of reducing toxicity of Tripterygii Radix et Rhizoma combined with Paeoniae Radix Alba from the perspective of transdermal transport. Method:The modified Franz diffusion cell was employed,taking in vitro abdominal skin of mice as transdermal barrier,with 20% ethanol physiological saline as receiving medium,the contents of triptolide(mobile phase of acetonitrile-water for gradient elution,detection wavelength at 220 nm),wilforgine and wilforine[mobile phase of acetonitrile-0.1% phosphoric acid(57:43),detection wavelength at 230 nm]in the receiving liquid was determined by HPLC,then the cumulative permeation quantity,steady transdermal rate and other penetration kinetic parameters were calculated. Result:Compared with Tripterygii Radix et Rhizoma cream,compatibility of Paeoniae Radix Alba and Tripterygii Radix et Rhizoma cream group could reduce cumulative penetration amounts of wilforgine and wilforine;among them,cumulative penetration amounts at 24 h of wilforgine and wilforine in cream with compatibility of Tripterygii Radix et Rhizoma and Paeoniae Radix Alba in 1:2 and 1:3 showed a significant decreasing trend;but the cumulative penetration amount of triptolide without the influence of compatibility. Conclusion:The compatibility of Tripterygii Radix et Rhizoma and Paeoniae Radix Alba can decrease the permeation of toxic components,and do not affect the permeability of triptolide,which is the main active substance of Tripterygii Radix et Rhizoma,so as to achieve the effect of reducing toxicity and increasing efficiency.关键词：Tripterygii Radix et Rhizoma;Paeoniae Radix Alba;transdermal absorption;compatibility;couplet medicines;triptolide;alkaloids3|2|6更新时间：2024-09-23
- 摘要：Objective:To screen and optimize the proper proportion of Ruditapes philippinarum(RP) and Schisandra chinensis fructus(SCF),and investigate its protective effect on liver injury in mice. Method:Taking activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in mice serum as indexes,uniform design was applied to investigate protective effect of RP and SCF with different proportion on liver injury mice induced by CCl4.The optimum proportion of RP to SCF was selected according to curve fitted by SAS 9.3 software.Validation of optimized proportion was implemented on the model of both CCl4 and alcohol induced mice liver injury at various dose levels. Result:Compatibility group of RP and SCF could significantly decrease the activities of AST and ALT in mice serum.The results of regression analysis showed that the activities of ALT and AST decreased with increasing of dose level of RP,and first decreased and then increased with increasing of dose level of SCF;however,there was no interactive effect between dose levels of RP and SCF.The optimum compatibility doses of RP and SCF were 0.57,0.30 g·kg-1,namely,the ratio of SCF to RP was 1:1.9.The validation experiment also showed that the compatibility group was better than the single group at the optimum dose. Conclusion:The compatibility of RP and SCF can protect liver.The study provides experimental basis for the development and utilization of RP resources.关键词：uniform design;Ruditapes philippinarum;Schisandra chinensis fructus;liver protection;carbon tetrachloride;alcoholic liver injury;marine medicine6|2|1更新时间：2024-09-23
- 摘要：Objective:To establish a quick,accurate and effective method for authenticity identification of Dioscoreae Rhizoma by using rapid and specific polymerase chain reaction (PCR) method. Method:Samples of Dioscoreae Rhizoma and its counterfeit products from different regions were collected. All samples were extracted for total DNA. Homologous comparative analysis of DNA barcoding rbcL fragments of Dioscoreae Rhizoma and its confused products was carried out. The differential primers for Dioscoreae Rhizoma were designed according to the identification site and the primer sequences were as follows Sy89:5'-AAGGACGATGCTACCACATCGACAC-3', Sy90:5'-ATCCCAATAGGGGACGGCCA-3'. The 2-step method was used for PCR amplification,and the influencing factors of the PCR reaction system and reaction procedures were investigated. Result:A rapid and specific PCR reaction procedure for Dioscoreae Rhizoma was obtained by optimizing the factors affecting PCR annealing temperature, annealing time, denaturation time and number of cycle times, as well as by examining different Mix. The PCR amplification reaction solution was 50 μL,including Premix TaqTM 25 μL,1.5 μL each for upstream and downstream primers,1.5 μL DNA template,and 50 μL sterile double distilled water. The PCR amplification procedure was as follows:pre-denaturation at 98℃ for 1 min,denaturation at 98℃ for 10 s, renaturation at 68℃ for 15 s,30 cycles, and extension at 72℃ for 30 s. The results of 1% agarose gel electrophoresis on PCR products showed that there was a clear band near 250 bp,and there was no band in the counterfeit products. Conclusion:Rapid and specific PCR method can be used to easily and quickly identify Dioscoreae Rhizoma,providing technical support for the field application of molecular identification of Chinese medicinal materials.关键词：rapid and specific polymerase chain reaction;Dioscoreae Rhizoma;molecular identification4|2|8更新时间：2024-09-23
- 摘要：Objective:To clone the full-length cDNA of 3-hydroxy-3-methylglutaryl CoA reductase(HMGR) gene from Gentiana rigescens,construct prokaryotic expression vector for expression in Escherichia coli, quantify the expressions and conduct bioinformatics analysis. Method:Polymerase chain reaction (PCR) technique was used for cloning the cDNA sequence of HMGR from G. rigescens. The pEASY-E1-HMGR expression vector was constructed to prokaryotic expression and the expression was induced by isopropyl β-D-thiogalactoside (IPTG). The obtained cDNA sequences were analyzed and their structure and function were forecasted by bioinformatics method. The expression levels of HMGR gene of roots,stems and leaves in different growth stages were analyzed by using Real-time quantitative PCR (Real-time PCR) in G. rigescens. Result:Two groups of HMGR gene were cloned,one group of HMGR cDNA full-length was 1 747 bp, open reading frame (ORF) of 1 697 bp, encoding 565 amino acids,named as GRHMGR-1;another group of HMGR cDNA full-length was 1 731 bp,ORF of 1 572 bp,encoding 523 amino acids,and named as GRHMGR-2. SDS-PAGE showed that the recombinant plasmid could successfully express the target proteins. Blastp found that GRHMGR-1 and GRHMGR-2 were closely related to the same genus plants G. lutea and G. macrophylla. The structure analysis results showed that both of them contained two HMG-CoA binding sites and two NADPH binding sites,implying that it may be localized on endoplasmic reticulum. RT-PCR showed that HMGR gene was expressed in roots, stems and leaves at four different growth stages, and the highest expression level was found in leaves. Conclusion:This was the first time to obtain cloned HMGR genes from G. rigescens. The results can provide a foundation for exploring the mechanism of gentiopicroside biosynthesis in G. rigescens.关键词：Gentiana rigescens;3-hydroxy-3-methylglutaryl CoA reductase;gene clone;prokaryotic expression;bioinformatics2|2|3更新时间：2024-09-23
- 摘要：Objective:To study the chemical constituents from Guizhou Miao medicine Reineckia carnea, and provide a scientific basis for explaining the pharmacological activities of R. carnea and its further development and utilization. Method:The 20 kg air-dried whole plant of R. carnea was extracted with 80% EtOH for three times by heating reflux, 2, 2 and 1 h respectively. These 3 extracts were combined, filtered, decompressed and concentrated into extract, which was then dissolved in water. Then the concentrated residue was successively partitioned with petroleum ether,EtOAc and n-BuOH to obtain 28 g petroleum ether fraction, 305 g EtOAc fraction and 812 g n-BuOH fraction. The chemical constituents of EtOAc and n-BuOH fractions were isolated and purified by repeated silica gel column chromatography,macroporous resin,MCI,ODS,Sephadex LH-20 gel column chromatography and Pre-HPLC,and their structures were identified on the basis of physical and chemical properties,HR-ESI-MS,NMR spectral analysis and literature data. Result:Thirteen compounds were isolated and identified as 2α,3β-dihydroxy-5α-pregn-16-en-20-one(1),(25R)-26-hydroxycholesterol (2),diosgenin (3),β-sitosterol (4),β-daucosterol (5),isorhamnetin-3-O-rutinoside (6),farrerol (7),luteolin (8),1,2,8-trihdroxy-5,6-dimethoxyxanthone (9),swertiamarin (10),di (2-ethylhexyl) phthalate (11),alternariol monomethyl ether (12), and N-trans-feruloyltyramine (13). Conclusion:Compounds 1,2,6-10,12,and 13 were isolated from this plant for the first time.关键词：Reineckia carnea;Miao medicine;farrerol;luteolin;swertiamine3|2|2更新时间：2024-09-23
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Analysis of Volatile Components in Different Jujubae Fructus by Accelerated Solvent Extraction GC-MS
摘要：Objective:To analyze the volatile components in Jujubae Fructus and identify the chemical compositions. Method:An accelerated solvent extraction (ASE) gas chromatography-mass spectrometry (GC-MS) method was applied for the analysis of volatile components in Jujubae Fructus. The relative percentage contents of the volatile components were determined by the peak area normalization method. Based on the extraction efficiency,two extraction methods including ASE and soxhlet extraction (SE) were investigated and compared for the extraction of volatile components,and the effects of two different solvents (80% ethanol and petroleum ether) were also investigated under the ASE method. In addition,the difference between the volatile components of the extracts before and after methyl esterification treatment was investigated, and the volatile components of the extracts from 9 kinds of Jujubae Fructus were compared by using principal component analysis. Result:The results showed that ASE method with 80% ethanol as the extract solvent was more efficient and safer. More 18 volatile components could be found after methyl esterification treatment in the extract of Hebei Xingtang Jujubae Fructus. The relative contents of unsaturated fatty acids in 9 kinds of Jujubae Fructus were up to 45.3%-70.3%,of which the relative content of hexadecenoic acid (methyl ester) was the highest and up to 24.0%-38.3%. Principal component analysis was applied for evaluation of the qualities through the common 18 volatile components in the extracts of 9 Jujubae Fructus samples from different regions. In this way,these 9 Jujubae Fructus samples from different regions were effectively distinguished and it was indicated that the best Jujubae Fructus was from Wuyi Mayazao,followed by Wuyi Xiaozao,Xianghe Xiaozao and Wuyi Dazao. Conclusion:There are certain differences in volatile components of Jujubae Fructus from different regions and varieties. This experiment can provide references for the development of jujube resources.关键词：Jujubae Fructus;volatile components;accelerated solvent extraction;principal component analysis;GC-MS2|2|0更新时间：2024-09-23 - 摘要：Objective:To compare the high performance liquid chromatography (HPLC) and infrared spectroscopy (IR) fingerprints of different fruit-shaped Gardenia jasminoides and analyze their differences. Method:The 48 batches of Gardeniae Fructus samples were processed to establish HPLC and IR fingerprints,and then the similarity evaluation and principal components cluster analysis were performed on Fujian,Henan,and Jiangxi six and seven edges Gardeniae Fructus samples. Result:HPLC fingerprints:22 common peaks were identified. The similarity was ranged from 0.789 to 0.896 among 6 edges Gardeniae Fructus in 48 samples,and 0.598 to 0.103 among seven edges Gardeniae Fructus. The similarity was low between six and seven edges Gardeniae Fructus in fruit shape, with great difference in components. In the principal components and cluster analysis,the six and seven edges Gardeniae Fructus were divided into two categories:Fujian six edges and Henan six edges Gardeniae Fructus were classified into one same category; Henan seven edges Gardeniae Fructus and Jiangxi seven edges Gardeniae Fructus were classified into one same category; the same fruit shape in different places was classified into one category. Infrared fingerprinting:In the cluster analysis,when the between-class distance was 1.35,Gardeniae Fructus samples were divided into 2 categories,six edges Gardeniae Fructus and seven edges Gardeniae Fructus; when the between-class distance was 0.875,Fujian 6edges Gardeniae Fructus and Henan six edges Gardeniae Fructus were classified into one category;when the between-class distance was 0.756,Henan seven edges Gardeniae Fructus and Jiangxi seven edges Gardeniae Fructus were classified into one category. Conclusion:This study shows that the contained components are obviously different in different fruit shaped Gardeniae Fructus,and the fingerprinting analysis can be used to evaluate different fruit shaped Gardeniae Fructus materials, providing reference for the clinical application of different fruit shaped Gardeniae Fructus in traditional Chinese medicine.关键词：Gardeniae Fructus;different fruit shape;fingerprint;HPLC;infrared2|2|3更新时间：2024-09-23
- 摘要：Objective:To separate the chemical constituents of n-butanol and dichloromethane ether fractions from Polygonatum cyrtonema and identify the structures of the compounds. Method:Rhizomes of P. cyrtonema were dried and crushed at 60℃ saccording to the traditional processing method of Jiangxi Jianchang,soaked by 70% ethanol for 1 hour,extracted for 2 times,filtered,and then extracted by three times with the two times volume of dichloromethane,ethyl acetate and n -butanol respectively. The extraction solution was decompressed and concentrated to obtain the dichloromethane fraction,ethyl acetate fraction and n -butanol fraction. The n -butanol and dichloromethane ether fractions from P. cyrtonema were isolated by macroporous adsorption resin chromatography,silica gelcolumn,re-crystallization,Sephadex LH-20 column,reversed phase high-performance liquid chromatography and their structures were identified according to physicochemical properties and spectral evidences. Result:Fourteen compounds were isolated from n -butanol and dichloromethane ether fractions of Polygonatum cyrtonema and elucidated as (25 S)spirostan-5-en-12-one-3-O-D -glucopyranosyl-(1→2)-O -[β-D -xylopyranosyl(1→3)]-O-β-D -glucopyranosyl(1→4)-β-D -galactopyranoside(1),(25 S)spirostan-5-en-12-one-3-O-β-D -glucopyranosyl-(1→2)-O -[β-D -glucopyranosyl-(1→3)]-O-β-D -glucopyranosyl-(1→4)-β-D -galactopyranoside(2),dibutyl phthalate(3),daucosterol(4), β -Sitosterol(5),hypofuran B(6),(25 R)-3-β-hydroxyspirost-5-en-12-one(7),(+)-medioresinol(8),polygonatine A(9),5-hydroxymethyl furfural(10),5-hydroxy maltol(11),2-(4-hydroxyphenyl)-ethanol(12),1H-indole-3-carboxaldehyde(13),and polygonatine B(14). Conclusion:Compounds 4,5,9 and 14 were firstly obtained from P. cyrtonema, and compounds 6,8,12 and 13 were isolated from the plants of Polygonatum for the first time,providing chemical basis and foundation for the comprehensive development and the research on active substances of natural plant.关键词：Polygonatum cyrtonema;chemical constituents;ethyl acetatefraction;n-butanol fraction4|2|8更新时间：2024-09-23
- 摘要：Objective:To investigate the anti-atherosclerosis mechanism of Sanhuang Xiexintang in activating blood and resolving stasis (SXTAR) in vitro. Method:The cell injury model was established with oxidized law-density lipoprotein(ox-LDL) in cultured human umbilical vein endothelial cells live (HUVECs) in vitro, and divided into control group, model group, and SXTAR 15.63,31.25,62.50 mg·L-1 groups. The HUVECs livability and the concerning markers in cell supernatant, like malondialdehyde(MDA), monocyte chemoattractant protein-1(MCP-1), intercellular adhesion molecule-1(ICAM-1), vascular cell adhesion molecule-1(VCAM-1), superoxide dismutase(SOD) and nitric oxide(NO), were detected; reactive oxygen species(ROS) was measured by fluorescence quantitation. Apoptosis rate and mitochondrial membrane potential were determined by flow cytometry. Western blot was employed for testing expressions of B-cell lymphoma-2(Bcl-2),Bcl-2 associated X protein(Bax),cysteine aspartate-specific protease-3(Caspase-3). Result:The survival rate of HUVECs in model group was significantly lower than that of control group. In the model group, MDA, MCP-1, ICAM-1, VCAM-1 were significant increases, SOD and NO content were decreased, apoptosis rate, ROS and expressions of Bax, Caspase-3, Caspase-9 were up-regulated, and expression of Bcl-2 was down(P<0.05,P<0.01)-regulated. SXTAR significantly increased the survival rate of HUVECs. Compared with the model group, SXTAR significantly decreased contents of MDA, MCP-1, ICAM-1 and VCAM-1.SOD and NO content were increased, apoptosis rate, ROS and expressions of Bax, Caspase-3, Caspase-9 was down-regulated, and expression of Bcl-2 was up-regulated(P<0.05,P<0.01). Conclusion:SXTAR can protect HUVECs injured by ox-LDL, which may be related with inhibiting the endothelial cell apoptosis, reducing inflammation and resisting oxidation.关键词：Sanhuang Xiexintang;atherosclerosis;oxidative stress;apoptosis4|2|10更新时间：2024-09-23
- 摘要：Objective:To investigate the inhibitory effect of oxymatrine on C-Jun N-terminal kinase(JUK)/extracellular-signalregulated kinase(ERK)/protein kinase B1(Akt1) signal in cardiac fibroblasts (CFs) proliferation induced by aldosterone (ALD) in vitro. Method:CFs were isolated and purified by differential adhesion. CFs were identified by anti-vimentin immunofluorescence staining. The experiment included control group (DMEM medium), ALD group (1×10-7 mol·L-1), low-dose OMT group (3.78×10-4 mol·L-1), high-dose OMT group (7.57×10-4 mol·L-1) and spironolactone group (Spir, 1×10-6 mol·L-1). The inhibitory effect of OMT on CFs proliferation was detected by methyl thinzolyl tetrazolium(MTT) assay.The mRNA and protein expressions of JNK, ERK and Akt1 were detected by Real-time PCR and Western blot. Result:MTT assay suggested that compared with the control group, ALD significantly induced CFs proliferation (P<0.05), OMT could significantly inhibit ALD-induced CFs proliferation (P<0.05). The mRNA and protein expressions of JNK, ERK and Akt1 were significantly increased by ALD compared with control group (P<0.05). OMT could inhibit the mRNA and protein expressions of JNK, ERK and Akt1 by Real-time PCR and Western blot. Conclusion:OMT could inhibit CFs proliferation induced by ALD, the mechanism may be correlated with the inhibition of JNK, ERK and Akt1 signal.关键词：aldosterone;oxymatrine;cardiac fibroblasts;mitogen-activated protein kinase(MAPK) signaling pathway;protein kinase B(Akt) signaling pathway3|2|1更新时间：2024-09-23
- 摘要：Objective:To observe the effect of extracts from on the sexual performance of rats with kidney-Yang deficiency induced by hydrocortisone. Method:Fifty male SD rats were randomly divided into normal control group, kidney-Yang deficiency model group, Cistanches Herba total oligosaccharide group, Cistanches Herba total polysaccharide group, and Cistanches Herba total phenylethanol glycosides, with 10 in each group. The animal model of kidney-Yang deficiency was prepared through intramuscular injection with hydrocortisone for 14 consecutive days. The medication groups were given aqueous solutions of total oligosaccharides of Cistanches Herba(103.28 mg·kg-1), total polysaccharides of Cistanches Herba (115.64 mg·kg-1), and total glycosides of phenylethanol of Cistanches Herba (201.48 mg·kg-1), according to the dose of 2.3 g·kg-1 of Cistanches Herba raw material once a day. After 14 days, erectile latency, indexes of mating ability (including capture latency, the number of captures, the ejaculation latency, the number of ejaculations), organ wet weight and berry sugar content, plasma cyclic adenosine monophosphate(cAMP)/cyclic guanosine monophosphate(cGMP) ratio, serum hormone testosterone (T), estradiol (E2), and follicle-stimulating hormone (FSH), luteinizing hormone (LH) and gonadotropin-releasing hormone (GnRH) levels were determined in each group of rats, and the testicular morphological changes were observed. Result:The extracts from different fractions of Cistanches Herba had the effect on erectile function, mating ability, organ wet weight and berry sugar content, plasma cAMP/cGMP value, serum hormone level, and testicular pathological changes in rats with kidney-Yang deficiency. Particularly, the extracts of total phenylethanol glycosides had the best effect, and could significantly reduce the erectile latency (P<0.05) and capture latency (P<0.01), significantly increase the times of capture (P<0.01), testis (P<0.05), seminal vesicles (P<0.01) wet weight and berry sugar content (P<0.05), significantly increase plasma cAMP/cGMP values (P<0.01), serum T (P<0.01), FHS (P<0.01), GnRH (P<0.05) levels, significantly reduce serum E2 (P<0.05), LH (P<0.05) levels, and significantly improve pathological morphology of testis in rats. Conclusion:Extracts from different fractions of Cistanches Herba can regulate hormone levels in rats with kidney-Yang deficiency induced by hydrocortisone and further improve their sexual ability. The total glycosides of phenylethanol can significantly improve the indexes related to sexual performance in rats with kidney-Yang deficiency.关键词：Cistanches Herba;kidney-Yang deficiency;sexual ability;phenylethanoid glycosides;oligosaccharides;polysaccharides4|2|16更新时间：2024-09-23
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Effect of Capsaicin on Proliferation of Human Breast Cancer MCF-7 Cells and p21 and FBI-1 Expression
摘要：Objective:To investigate the effect of capsaicin on proliferation of human breast cancer MCF-7 cells and its possible molecular mechanism. Method:Capsaicin groups with different concentrations and blank group were set up. The cell viability was detected by cell count kit-8 (CCK-8) assay after MCF-7 cells were treated with capsaicin (50,100,150,200,250,300 μmol·L-1) for 24, 48,72 h. The cell colony formation ability was verified by colony formation assay after MCF-7 cells were treated with capsaicin(100,150,200 μmol·L-1) for 18 h and then incubated in complete medium for 14 days. The mRNA expression levels of cyclin-dependent kinase inhibitor p21 and factor that binds to the inducer of short transcripts of human immunodeficiency virus-1 (FBI-1) were measured by Real-time polymerase chain reaction (Real-time PCR) after MCF-7 cells were treated with capsaicin (100,150,200 μmol·L-1) for 24 h. The protein expression levels of p21 and FBI-1 were measured by Western blot after MCF-7 cells were treated with capsaicin (100,150,200 μmol·L-1) for 24 h. Result:As compared with the blank group, capsaicin (50,100,150,200,250,300 μmol·L-1) significantly inhibited the cell viability of MCF-7 cells (P<0.05,P<0.01) in dose and time dependent manners. Capsaicin (100,150,200 μmol·L-1) also markedly inhibited the cell colony formation ability (P<0.01). In addition, capsaicin (100,150,200 μmol·L-1) up-regulated the mRNA and protein expressions of p21 and down-regulated mRNA and protein expressions of FBI-1 (P<0.01). Conclusion:Capsaicin inhibits cell proliferation of MCF-7 cells, and the possible mechanism may be related to up-regulation of p21 and down-regulation of FBI-1 at both mRNA and protein levels.关键词：capsaicin;breast cancer;proliferation;cyclin-dependent kinase inhibitor p21(p21);factor that binds to inducer of short transcripts-1(FBI-1)2|2|4更新时间：2024-09-23 - 摘要：Objective:To investigate the effect of Linggui Zhugan Tang on the hepatic steatosis in leptin-deficient mice, in order to explore the possible mechanism of Linggui Zhugan Tang on farnesoid X receptor(FXR)fibroblast growth factor 15(FGF15)-small heterodimer partner(SHP) pathway. Method:The mice were divided into five groups:normal group, model group, high-dose Linggui Zhugan Tang group(23.1 g·kg-1), low-dose Linggui Zhugan Tang group(7.7 g·kg-1), and positive drug group(0.002 6 g·kg-1). After 4 weeks of treatment, the weight of body was recorded, and the liver organ coefficient was calculated. The effect of Linggui Zhugan Tang on hepatic steatosis was investigated by htoxylin eosin(HE) and oil red O staining. The protein expressions of FXR, FGF15, and SHP in the liver were detected by immunofluorescence staining and Western blot. Result:Compared with normal group, the body weight and liver index were significantly increased, with serious hepatic steatosis, while the protein expression level of FXR, FGF15 and SHP were significantly lower in ob/ob mice(P<0.01). Compared with model group, the body weight and liver index were declined, whereas the protein expressions of FXR, FGF15, and SHP were significantly up-regulated after treatment(P<0.05,P<0.01). Conclusion:Linggui Zhugan Tang could improve the hepatic steatosis in ob/ob mice, which may be related to its effect on FXR/FGF15/SHP pathway.关键词：Linggui Zhugan Tang;leptin-deficient;nonalcoholic fatty liver disease;farnesoid X receptor (FXR);warming Yang to resolve fluids retention2|2|3更新时间：2024-09-23
- 摘要：Objective:To investigate the effect of Shenling Baizhusan (SLBZS) in repairing the skin mechanical barrier function in mice and the expression of β-glucocerebrosidase (β-GCase) and acid sphingomyelinase (ASM) related to ceramide salvage pathway. Method:A total of 50 SPF ICR mice were randomly divided into the blank control group, the model group, the low-dose SLBZS group (10 g·kg-1), the middle-dose SLBZS group (20 g·kg-1) and the high-dose SLBZS group (40 g·kg-1). The tape-stripping method was used to replicate the mouse model of the dysfunction of the skin mechanical barrier. The low-dose group, the middle-dose group and the high-dose group were given the corresponding doses of SLBZS by gavage 8 d before modeling. The other groups were given distilled water once a day for 13 days. The transepidermal water loss (TEWL), skin hydration and skin pH were measured. The pathological changes of skin tissue were observed by hematoxylin-eosin (HE) staining. The protein expressions of β-GCase, ASM were detected by immunohistochemistry. β-GCase and ASM mRNA expressions in skin tissues were detected by Real-time PCR. Result:Compared with the blank control group,the level of TEWL and pH were significantly increased in the model group (P<0.05), and the skin hydration was decreased in the model group (P<0.01). The expression of β-GCase was decreased in the model group (P<0.05). Compared with the model group, each dose of SLBZS could decrease the levels of TEWL and pH (P<0.01), and increase the skin hydration (P<0.01). The histopathological changes of the skin were improved to varying degrees. The middle-dose SLBZS group could up-regulate the expression of β-GCase (P<0.05,P<0.01). Conclusion:SLBZS can repair the mechanical barrier function of skin, which may be related to the up-regulation of β-GCase expression and increase of ceramide content.关键词：Shenling Baizhusan;skin mechanical barrier function;β-glucocerebrosidase;acid sphingomyelinase2|1|3更新时间：2024-09-23
- 摘要：Objective:To study the effect of Polygoni Hydropiperis Herba on diarrhea mice induced by Escherichia coli, in order to explore its possible mechanism. Method:A total of 160 KM mice were randomly divided into blank group, model group, berberine group and drug groups (high-dose, medium-dose and low-dose Polygoni Hydropiperis Herba). All of the other groups, except for the blank group, were injected intraperitoneally with E. coli. The blank group and the model group were given normal saline intragastrically, drug groups were administrated with Polygoni Hydropiperis Herba extract at the dose of 2.5, 5, 7.5 g·kg-1 respectively, and the berberine group was given 0.025 g·kg-1 of berberine.On the 1st, 2nd, 4th and 6th days after intraperitoneal injection with E. coli, 5 mice were randomly selected from each group. Their duodenum histopathological features were observed under optical microscope. The content of interleukin-1β (IL-1β) and tumor necrosis factor-α(TNF-α) was determined by enzyme linked immunosorbent assay (ELISA), the mRNA expression levels of transforming growth factor-β1 (TGF-β1), and epidermal growth factor receptor (EGFR) Smad3 and Smad7 were detected by real-time fluorescence quantitative polymerase chain reaction (Real-time PCR). Result:The duodenal intestinal mucosal injury in mice could be significantly alleviated by Polygoni Hydropiperis Herba. ELISA results showed that the content of TNF-α and IL-1β in the model group significantly increased compared with the blank group(P<0.01). The content of TNF-α and IL-1β in the Polygoni Hydropiperis Herba groups (at dose of 5 and 7.5 g·kg-1) was reduced obviously compared with the model group (P<0.05,P<0.01). Real-time PCR results showed that the mRNA expressions of TGF-β1, EGFR in the model group significantly increased (P<0.05,P<0.01), and mRNA expression of Smad7 in the model group was significantly reduced (P<0.01) compared with the bland group. After administration,Polygoni Hydropiperis Herba and berberine can regulate mRNA expressions of TGF-β1, EGFR, Smad3 and Smad7 in duodenum of diarrhea mice(P<0.05), in a dose-dependent manner. Conclusion:Extract of Polygoni Hydropiperis Herba has an obvious effect on diarrhea mice induced by E. coli, and the mechanism may be related to reducing the release of intestinal inflammatory cytokines, alleviating the intestinal mucosal barrier, and intervening TGF-β/Smads signal pathway.关键词：Polygoni Hydropiperis Herba;Escherichia coli-induced diarrhea;tumor necrosis factor-α(TNF-α);interleukin-1β(IL-1β);transforming growth factor-β1 (TGF-β1);epidermal growth factor receptor (EGFR);TGF-β/Smads signal pathway2|2|5更新时间：2024-09-23
- 摘要：Objective:To study the effect of Tangweian decoction on gastric emptying in diabetic rats with gastrointestinal diseases, so as to explore its mechanism. Method:Fifty 2-3-month-old male Wistar rats were collected as subjects. Ten of them were randomly selected as normal control group (CON), and the other 40 were made diabetic through intraperitoneal injection with streptozotocin(STZ,50 mg·kg). Diabetes was verified after 1 week, and the animals with a blood sugar level higher than 16.9 mmol·L-1 were considered as diabetic for the experiments. The 40 rats were randomly divided into diabetic control group (DM), high-dose Tangweian decoction group (T1), middle-dose group (T2), and low-dose group (T3)(15,10,5 g·kg-1). CON group and DM group were treated with distilled water by gavage. After 8 weeks, the gastric emptying rate was determined by single photon emission computed tomography (SPECT). The immunohistochemistry was used to observe the changes of Cajal interstitial cells in the gastric myenteric layer. The hypothalamus substance P protein expression was detected by Western blot. Result:Compared with the CON group, the gastric emptying rate in DM group, T1 group, T2 group and T3 group decreased significantly (P<0.01); the Cajal interstitial cells in gastric myenteric layer decreased significantly (P<0.05, P<0.01); the hypothalamus protein expression substance P decreased significantly (P<0.05, P<0.01). Compared with DM group, the gastric emptying rate and the Cajal interstitial cells in T1 and T2 groups were increased significantly (P<0.05, P<0.01), while the expression of P substance in hypothalamus increased significantly(P<0.05, P<0.01). For T3 group, the gastric emptying rate, Cajal interstitial cells and the expression of P substance in hypothalamus were increased, but with no statistically significant difference. Conclusion:Gastric emptying rate, Cajal interstitial cells in gastric myenteric layer, and the expression of substance P in hypothalamus were decreased in rats with gastrointestinal disease. Tangweian decoction has the effect in promoting the gastric emptying rate of diabetic rats with gastrointestinal diseases rats and improving gastrointestinal motility by increasing Cajal interstitial cells in gastric myenteric layer and substance P levels.关键词：Tangweian decoction;gastric emptying rate;Cajal interstitial cells;substance P2|2|2更新时间：2024-09-23
- 摘要：Objective:To investigate the effect of luteolin on the proliferation and apoptosis of T cells in vitro. Method:Blank group,ConA control group and 3 luteolin groups (2.5,5,10 mg·L -1) were set up. The effect of luteolin on the proliferation of T lymphocyte in the 5 groups was detected by cell counting kit-8(CCK-8).TUNEL and Flow Cytometry were used to detect apoptosis condition in each group. Western blot method was used to detect the expression of B-cell lymphoma-2(Bcl-2) and Bcl-2 associated X protein (Bax) in each group. Result:Number of T cell in 3 different doses of luteolin were decreased significantly compared with 2 control groups(P<0.01),with statistically significant differences. TUNEL and flow cytometry indicated that, compared with ConA groups, apoptotic percentages of the 3 luteolin groups were increased under fluorescence microscope(P<0.01), with statistically significant differences. Among apoptosis-related proteins, Bax was up-regulated, but Bcl-2 was down-regulated in 10 mg·L-1 group according to Western blot. Conclusion:The 2.5,5,10 mg·L-1 of luteolin can inhibit the proliferation of T cell and induce its apoptosis. 10 mg·L-1 of luteolin may mainly induce the apoptosis by down-regulating expression of Bcl-2 and up-regulating expression of Bax in mitochondria apoptosis pathway, and 2.5,5 mg·L-1 of luteolin may induce the apoptosis through other pathways.关键词：luteolin;T lymphocyte;apoptosis;B-cell lymphoma-2(Bcl-2);Bcl-2-associated X protein(Bax)2|2|1更新时间：2024-09-23
- 摘要：Objective:To study the effect of ginsenoside Rg5(12.5,25,50 μmol·L-1) on the invasion and migration and the expressions of microRNA-125b(miR-125b),StAR-related lipid transfer domain protein 13(STARD13) and neuraminidase 1 (NEU1)in gastric cancer BGC-823 cell, in order to investigate the possible mechanism of ginsenoside Rg5 in inhibiting the invasion and migration of gastric cancer. Method:Transwell was used to detect the invasion and migration of gastric cancer BGC-823 cell, Real-time PCR was used to detect the mRNA expressions of miR-125b,STARD13 and NEU1, and Western blot was used to detect the protein expressions of miR-125b,STARD13 and NEU1. Result:Middle and high-dose ginsenoside Rg5 could effectively inhibit the invasion and migration of BGC-823 cells. Compared with the blank group, the relative expression of miR-125b in low-dose group was decreased, the relative expressions of STARD13 and NEU1 mRNA in the low-dose group were increased; the relative expression of miR-125b in middle and high-dose groups was decreased, the relative expressions of STARD13 and NEU1 mRNA in middle and high-dose groups were increased(P<0.01). Compared with the blank group, the relative expression of STARD13 protein in low and middle-dose groups were increased, and NEU1 protein in low-dose group was increased; the relative expression of STARD13 protein in high-dose group was increased(P<0.05), and NEU1 protein in middle and high-dose groups was increased(P<0.01). Conclusion:Ginsenoside Rg5 may inhibit the invasion and migration of gastric cancer BGC-823 cells by decreasing the expression of miR-125b and up-regulating the expressions of miR-125b target genes STARD13 and NEU1.关键词：ginsenoside Rg5;gastric cancer;BGC-823 cell;invasion;metastasis2|1|3更新时间：2024-09-23
- 摘要：Objective:To evaluate the genotoxicity of Cinnabaris by using in vitro CHL cell and in vivo Hamster (Phodopus sungorus) chromosome aberration tests. Method:In vivo chromosome aberration test, hamsters (P. sungorus) were orally given cinnabar suspension at doses of 2.0, 4.0, 8.0 g·kg-1 respectively for 5 days. They were intraperitoneally injected with colchicine 2 hours before being put to death. Bone marrow cells were taken to prepare chromosome smears. Mitotic index of bone marrow cells and types of chromosome aberration in 100 metaphase cells per animal were observed under the microscope oil lens. In CHL cell chromosome aberration test, cinnabar extracts were used to react with cells, with the final concentration of 6.3, 12.5, 25.0 g·L-1, and cultured for 24 or 48 h. Colchicine was added and cultured for 4 hours. The cells were collected to prepare chromosome smears. Types of chromosome aberration in 200 metaphase CHL cells per dose were observed under the microscope oil lens. Result:① Obvious chromosome aberrations were observed at doses of 12.5,25.0 g·L-1 in CHL cell chromosome aberration test(P<0.05,P<0.01). ② Compared with the negative control, the chromosome aberration rates in both in vivo chromosome aberration test and in vitro CHL cell chromosome aberration test after administration showed no statistically significant difference, with no dose-effect relations. Conclusion:① Cinnabar can cause chromosome aberrations in vitro at the dose of 12.5 g·L-1 in this test.However, there was no positive result in the cinnabar suspension chromosomal aberration test at the dose of 8.0 g·kg-1. ② Hamster (P. sungorus) in vivo chromosome aberration test can be used as an experimental method for evaluating the genotoxicity of new traditional Chinese medicines.关键词：genotoxicity;chromosome aberrations;Cinnabaris;in vivo;in vitro;hamster (Phodopus sungorus);CHL cell line4|2|0更新时间：2024-09-23
- 摘要：Objective:To observe the effect of modified Xiaofengsan on fractional concentration of exhaled nitricoxide (FeNO),substance P (SP) and vasoactive intestinal peptide (VIP) in plasma of patients with persistent asthma. Method:Totally 80 cases in line with the inclusion criteria were collected and randomly divided into control group and treatment group. Patients in control group were given Budesonide/formoterol(160 μg/4.5 μg),while patients in treatment group were given modified Xiaofengsan combined with Budesonide/formoterol for 1 months. Before and after treatment, forced expiratory volume in first second(FEV1),percentage of forced expiratory volume in first second and forced vital capacity(FEV1/FVC),percentage of forced expiratory volume in first second and its predicted value(FEV1%),variety ratio of peak expiratory flow,FeNO and the levels of SP and VIP in plasma were measured respectively. The adverse reactions of the two groups were recorded at the same time. Result:Compared with control group,FEV1,FEV1/FVC,FEV1%, VIP in plasma in treatment group were higher(P<0.05), while PEF variety ratio, FeNO and the level of SP in plasma were lower (P<0.05)after treatment. Compared with control group,the incidence rate of the adverse reactions in treatment group was lower (P<0.05). Conclusion:Modified Xiaofengsan combined with Budesonide/formoterol is more effective than Budesonide/formoterol in improving pulmonary function,regulating the neurogenic airway inflammation and lowering the incidence rate of the adverse reactions. It is worth promotion and application.关键词：bronchial asthma;substance P(SP);vasoactive intestinal peptide(VIP);fractional concentration of exhaled nitricoxide(FeNO);traditional Chinese treatment2|2|2更新时间：2024-09-23
- 摘要：Objective:To observe the clinical efficacy, recurrence rate and safety of modified Xiao Chaihutang, a classical prescription of traditional Chinese medicine, in the treatment of subacute thyroiditis (SAT). Method:Ninety eligible SAT patients were randomly divided into treatment group (46 cases) and control group (44 cases). The patients in treatment group were treated with Xiao Chaihutang plus forsythia decoction, 1 dose per day, while the patients in control group took the therapy of glucocorticoids only, with a treatment course of 12 weeks for both groups. The clinical efficacy, pain relief, time to pain disappearance, fever clearance time, recurrence rate and adverse events, long term incidence of hypothyroidism. Result:After 2 weeks' treatment, both treatment group and control group showed significant decrease in erythrocyte sedimentation rate (ESR)(P<0.01), and ESR in treatment group was slightly higher than that in control group, with no statistical differences between two groups. After 12 weeks' treatment, both groups showed significant decrease in ESR(P<0.01), and the ESR in treatment group was significantly lower than that in control group (P<0.05); the time to pain relief in the treatment group was longer than that in control group (P<0.05), and the time to pain disappearance was shorter than that in control group, with no statistical differences; the fever clearance time in treatment group was slightly shorter than that in control group with no statistical differences between two groups. The total effective rate was 97.83% in treatment group, higher than 83.33% in control group (P<0.05). The incidence of adverse events was 2.17% in treatment group, slightly lower than 6.82% in control group, but with no statistical differences between two groups. 6 months after treatment, the recurrence rate was 2.17% in treatment group, lower than 14.29% in control group. The incidence of hypothyroidism was 4.35% in treatment group, lower than 9.52% in control group, but with no statistical difference between the two groups. Conclusions:As compared with glucocorticoids therapy, modified Xiao Chaihutang plus forsythia has more significant efficacy in the treatment of SAT, showing better compliance, higher safety and lower recurrence rate.关键词：subacute thyroiditis;Xiao Chaihutang;forsythia;glucocorticoids3|2|1更新时间：2024-09-23
- 摘要：Objective:To investigate the effect of Gualou Honghua Gancao Tang on inflammatory factors and T lymphocyte subsets in herpes zoster with stagnated heat of the liver meridian. Method:A total of 162 cases in our hospital were divided into control group and treatment group by random number table method. The control group was treated with oral aciclovir tables and mecobalamin capsules, and treatment group was given Gualou Honghua Gancao Tang in addition to the therapy of control group for two week. The clinical effect and inflammatory factors and T lymphocyte subsets levels were observed. After 6 months of follow-up, postherpetic neuralgia (PHN) incidence was recorded. Result:After treatment, the clinical effective rate of treatment group was significantly higher than that of control group (P < 0.05). The blister time, scab time, scab removal time and pain relief time in treatment group were significantly shorter than those of control group (P<0.05). The visual analogue scale (VAS) score at 7,14 d after treatment was significantly lower than that before treatment (P<0.05), and the treatment group was significantly lower than that of control group (P<0.05). After 6 months of follow-up, the incidence of PHN in treatment group was lower than that in control group (P<0.05). After treatment, the interleukins (IL-4), IL-6, tumor necrosis factor (TNF)-α and CD8+ levels in treatment group were decreased and significantly lower than those of control group (P<0.05), while the CD4+ and CD4+/CD8+ levels were increased compared with before treatment and significantly higher than those of control group (P<0.05). Conclusion:Gualou Honghua Gancao Tang could improve the clinical efficacy of herpes zoster patients with stagnated heat of the liver meridian by enhancing the immune function and inhibiting the inflammatory reaction.关键词：Gualou Honghua Gancao Tang;stagnated heat of liver meridian;herpes zoster;T lymphocyte subsets;inflammatory factors2|2|4更新时间：2024-09-23
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Effect and Safety of Yishen Gukang Decoction Combined with Oxycontin in Treating Cancer Somatic Pain
摘要：Objective:To observe the efficacy and safety of Yishen Gukang decoction combined with oxycontin in treating cancer patients with somatic pain. Method:A total of 100 patients with cancer somatic pain treated in Wangjing Hospital from January 2015 to May 2017 were randomly divided into treatment group and control group, with 50 cases in each group. In the first week, both groups were given oxycontin. In the second week, treatment group was additionally given Yishen Gukang decoction, while control group was given placebo. The therapeutic course lasted for 7 days. karnofsky(KPS) score, Karnofsky score, oxycontin dosage and the adverse reactions of the two groups were observed before and after treatment. Result:The total effective rate of treatment group was 94%, with an efficiency rate of 70%; and the total effective rate of control group was 88%, with an efficiency rate of 40%. The curative effect of treatment group was significantly better than that of control group (P<0.01). Moreover, treatment group was better than control group in relieving moderate pain (P<0.01). In Karnofsky score, oxycontin dosage and adverse reactions, treatment group was better than control group (P<0.05). Conclusion:Yishen Gukang decoction can effectively reduce the scores of cancer somatic pain and the oxycontin dosage, and improved the quality of patients' life. It is worth of further clinical promotion and study.关键词：Yishen Gukang decoction;cancer somatic pain;oxycontin;clinical research;adverse reactions3|2|0更新时间：2024-09-23 - 摘要：Objective:To explore the clinical effect of Jichuanjian in treatment of elderly with chronic functional constipation (CFC). Method:The 118 elderly with CFC in our hospital from February 2016 to February 2018 were selected as research objectives, and divided into two groups according to random number table. The patients in control group (59 cases) received prucalopride succinate tablets, and prucalopride succinate tablets combined with Jichanjian was given to the patients in observation group (59 cases). All patients were treated for 1 month. The clinical efficacy, gastrointestinal function indicators, intestinal neurotransmitters[nitrogen monoxide (NO), serotonin (5-HT), substance P (SP)] and intestinal flora before and after treatment, as well as adverse reaction were compared between two groups. Result:After 1 month treatment, the overall effective rate was 93.2%(55/59) in observation group, significant higher than that in control group[78.0%(46/59), P<0.05]. The whole gastrointestinal tract discharge rate and gastric emptying rate after treatment were significant higher than those before treatment in both groups (P<0.05), and the increase in observation group was more obvious than that in control group(P<0.05). As compared with those before treatment, the serum NO levels were significant lower (P<0.05), while serum SP, 5-HT concentrations were significant higher after treatment in both groups (P<0.05), and improvement in observation group was more obvious than that in control group(P<0.05). The number of gastrointestinal anaerobes lactobacillus and bifidobacteria was significant higher than that before treatment after treatment in both groups (P<0.05), while the number of aerobic bacteria yeast and enterobacteria was significant lower than that before treatment (P<0.05), and the improvement in observation group was more obvious than that in control group(P<0.05). As compared with the conditions before treatment, the scores for symptoms of defecation (fecal characteristics, defecation interval, difficulty in defecation, and incomplete defecation) were significantly reduced in both groups after treatment (P<0.05). As compared with control group, defecation symptom scores of the patients in observation group were decreased more significantly (P<0.05). In addition, there was no significant difference in adverse reaction rate between observation group[6.8% (4/59)] and control group[3.4% (2/59)]. Conclusion:The use of Jichuanjian adjuvant therapy can significantly relieve the symptoms in elder patients with CFC, enhance gastrointestinal function, regulate the expression level of intestinal neurotransmitters, and maintain intestinal microbiota homeostasis, with significant clinical effect and high safety.关键词：chronic functional constipation;Jichuanjian;gastrointestinal function;intestinal neurotransmitters;intestinal flora3|2|8更新时间：2024-09-23
- 摘要：Objective:To observe the Buyang Huanwu Tang combined with Bazhentang on blood glucose, islet function, nerve conduction function and serum vascular endothelial (VEGF), nerve growth factor (NGF) and basic fibroblast growth factor (bFGF) as well as the expression of inflammatory factors in patients with diabetic muscle atrophy, and investigate its efficacy and mechanism. To explore the therapeutic effect and mechanism. Method:The 78 patients with diabetic myatrophy were randomly divided into control group and observation group according to the random digital table method, with 39 cases in each group. On the basis of routine treatment, the control group was additionally treated with anti-obstructive aerobic exercise, and the observation group received Buyang Huanwu Tang combined with Bazhentang and anti-obstructive aerobic exercise. The course of treatment was 3 months in both groups. The clinical efficacy of the two groups was evaluated, and the levels of blood glucose, nerve electrophysiology, insulin resistance and serum VEGF, bFGF, NGF and inflammatory factors in two groups before and after treatment were measured and compared. Result:The effective rate of symptom score was 97.4% and the effective rate of weight improvement was 94.9% in the observation group, significantly better than 74.4% and 76.9% in control group (P<0.05). After treatment, the motor nerve conduction velocity (MCV) of median nerve and common peroneal nerve as well as sensory nerve conduction velocity (SCV) of median nerve and the sural nerve were faster than the conditions before treatment in both groups (P<0.05), and the effect in observation group was better than that in control group (P<0.05). After treatment, the two hours postprandial blood glucose (2 hPBG) and Hemoglobin A1C (HbA1c) were significantly lower than those before the treatment in both groups (P<0.05). After treatment, the levels of homeostasis model assessment-insulin resistance index (HOMA-IR), interleukin (IL) -6 and tumor necrosis factor (TNF) -α were significantly lower than those before the treatment in both groups, and the insulin sensitive index (ISI) in observation group was significantly higher than that before the treatment (P<0.05). After treatment, the levels of HOMA-IR, IL-6 and TNF-α in observation group were significantly lower than those in control group (P<0.05). The levels of VEGF, NGF and bFGF were significantly higher after treatment in both groups (P<0.05), and the observation group was higher than that of control group (P<0.05). Conclusion:Buyang Huanwu Tang combined with Bazhentang and anti-obstructive aerobic exercise can better improve blood glucose level, improve insulin resistance and relieve clinical symptoms in patients with diabetic muscular atrophy, and its mechanism may be related to improving the inflammatory state of the body and improving the levels of VEGF, NGF and bFGF.关键词：diabetic muscle atrophy;Buyang Huanwu Tang combined with Bazhentang;anti-obstructive aerobic exercise;nerve growth factor;basic fibroblast growth factor3|2|3更新时间：2024-09-23
- 摘要：Objective:To observe the clinical efficacy of addition and subtraction treatment of Danggui Sinitang combined with fumigation and washing with medicinal solution for motor function of children with spastic cerebral palsy, and to investigate its' effect on pro-inflammatory factor, anti-inflammatory factor and hemorheology. Method:One hundred and eight patients were randomly divided into control group (54 cases) and observation group (54 cases) by random number table produced by SAS software at 1:1.Patients in control group got rehabilitation training according to Guidelines for the Rehabilitation of Cerebral Palsy in China, accompanied by the treatment of Bohath and Vojta, 40 min/time, 1 time/day, 6 times/week. Based on the treatment in control group, patients in observation got additional treatment of addition and subtraction of Danggui Sinitang combined with fumigation and washing with medicinal solution, 30 min/time, 1 time/day, 6 times/week. The treatment course was 6 weeks in both groups. Before and after treatment, gross motor function measure (GMFM) and fine motor function measure (FMFM) were conducted; modified Ashworth scale (MAS) and clinic spasticity index (CSI) were evaluated both before and after treatment; and levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-10 and hemorheological indexes were detected. Result:After treatment, scores of three functional areas of A, B, C of GMFM scale and the total score in observation group were higher than those in control group (P<0.01). And the scores of functional areas of B, C, D, E of FMFM scale and the total score were also higher than those in control group (P<0.01). Classifications of MAS and CSI in observation group were less than those in control group (Z=2.465, P<0.05 and Z=1.975, P<0.05). Levels of TNF-α and IL-6 were lower than those in control group, and level of IL-10 was higher than that in control group (P<0.01). The whole blood low and high shear viscosity, plasma viscosity, fibrinogen, and platelet aggregation rate were lower than those in control group (P<0.01). Conclusion:Based on the treatment of Bohath and Vojta, treatment of addition and subtraction of Danggui Sinitang combined with fumigation and washing with medicinal solution can improve GMFM, FMFM and muscle strength, relieve the degree of spasm, and can regulate pro-inflammatory factor, anti-inflammatory factor and hemorheology.关键词：cerebral palsy;Danggui Sinitang;fumigation and washing with medicinal solution;motor function;inflammatory factor;hemorheology2|2|3更新时间：2024-09-23
- 摘要：Objective:To observe the efficacy of Runing granule in treating hyperplasia of mammary glands with syndrome of liver qi depression and phlegm stasis, and investigate its effects on progesterone(P), estradiol (E2), prolactin (PRL), luteinizing hormone (LH), follicle stimulating hormone (FSH) and angiogenic factors angiopoietin(Ang) -2, hypoxia inducible factor (HIF) -1α, vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF). Method:One hundred and twenty-two female patients with hyperplasia of mammary glands were selected and randomly divided into treatment group and control group with 61 cases in each group according to random number table. Both groups were orally with Xiaoyao wan(8 pills/time and tid). The patients in control group were additionally treated with Tamoxifen citrate tablets (20 mg/time and bid). Based on the treatment in control group, the patients in treatment group were additionally treated with Runing granule (1 bag/time and tid). Both groups were treated for 8 weeks. Scores of mammary gland hyperplasia symptoms, simple McGill pain questionnaire(SF-MPQ), and symptoms of syndrome of liver Qi depression and phlegm stasis and efficacy were compared between two groups. Serum levels of P, E2, PRL, LH, and FSH as well as Ang-2, HIF-1α, VEGF, and bFGF were detected in both groups. Result:After treatment, scores of hyperplasia of mammary glands for breast lump hardness and gland tumor size, as well as SF-MPQ scale visual analogue scale (VAS), present pain intensity (PPI), pain rating index (PRI) of treatment group were evidently lower than those in control group (P<0.01). After treatment, scores of symptoms of syndrome of liver qi depression and phlegm stasis (depression and irritability, chest distress, insomnia and dreamy sleep, upset and bitter mouth) of treatment group were evidently lower than those of control group (P<0.01). Total clinical effective rate of treatment group was 98.31%, obviously higher than 86.89% in control group (χ2=4.319, P<0.05). After treatment, serum levels of E2, PRL, and LH in treatment group were remarkably lower, while P and FSH level were obviously higher than those in control group (P<0.01). After treatment, serum levels of ngiogenic factors Ang-2, HIF-1α, VEGF, and bFGF in treatment group were obviously lower than those in control group (P<0.01). Conclusion:Based on routine therapy, the efficacy of additional Runing Granule in treating hyperplasia of mammary glands with syndrome of liver qi depression and phlegm stasis is significant, which may be correlated with regulation of endocrine hormones (P,E2, PRL, LH, and FSH) and angiogenic factors (Ang-2, HIF-1α, VEGF, and bFGF).关键词：Runing Granule;hyperplasia of mammary glands;syndrome of liver qi depression and phlegm stasis;endocrine hormones;angiogenic factors3|2|0更新时间：2024-09-23
- 摘要：Coicis Semen Praeparatum decoction pieces is a traditional Chinese medicine(TCM) processed specialty products of Zhangbang,based on the theory of TCM processing,this processed product of Coicis Semen was processed by infusing,steaming,stir-frying,etc.Its property has changed from cool to tepidity,which has a better effect of invigorating the spleen and removing dampness.Coicis Semen Praeparatum decoction pieces is widely used in some clinic departments,it can be used to treat rheumatic and rheumatoid arthritis,functional dyspepsia,skin diseases,digestive tract tumors and other diseases.In order to further improve the understanding of Coicis Semen Praeparatum decoction pieces by clinicians,our group and relevant experts jointly developed expert consensus of Coicis Semen Praeparatum decoction pieces in order to promote the development of local characteristic decoction pieces.关键词：Coicis Semen Praeparatum;consensus;clinical application3|2|4更新时间：2024-09-23
- 摘要：Chinese medicinal materials are varied and multi-source,large difference in quality makes it difficult to ensure the safety and efficacy of clinical medications.Traditional identification methods have certain limitations,in some cases difficult to accurately identify. In order to ensure the safe and effective of traditional Chinese medicine (TCM),TCM identification has become one of the most important research topics,which is a key technical requirement urgently needed to be solved in the current Chinese medicine business. DNA gene identification is a rapid and accurate identification of Chinese medicinal materials with the genetic level,includes DNA molecule genetic markers,DNA hybridization and DNA barcoding molecular identification method particularly the most widely used one is DNA barcoding molecular identification method.DNA barcode is a technique for identifying species by using standard DNA regions. The 2015 edition of the Chinese Pharmacopoeia has record the DNA barcode technical guidelines,which has established identify genes with COI gene-dominated and ITS2 gene-supplemented animal medicine DNA barcoding system,identify genes with ITS2/ITS gene-dominated and chloroplast psbA-trnH gene-supplemented plant medicine DNA barcoding system. The application of DNA Barcoding provides a more efficient and rapid method for the identification of TCM,and effectively applied in the identification of multi-primary Chinese herbal medicines. This article briefly outlines the principles,purpose and significance,selection of standard gene fragments of DNA barcodes,focuses on the application in identification of TCM,as well as the limitations. At last,the application forecast was prospected,which was expected to provide reference for Chinese medicine identification.关键词：DNA barcoding;Chinese medicinal materials;DNA barcode screening;mitochondrial gene;nuclear gene;chloroplast gene3|2|18更新时间：2024-09-23
- 摘要：Codonopsis Radix is a perennial herb of Campanulaceae,it has the effect of invigorating the spleen and benefiting the lung,nourishing blood and producing fluid;and it is a common clinical Chinese medicine.After undergoing changing,Codonopsis Radix has formed major production areas in Shanxi,Shaanxi,Gansu,Sichuan and Hubei provinces,and each major producing area has taken measures according to local conditions,to form a unique product specification,processing technology and process.Regarding the processing methods of Codonopsis Radix,the ancient literature since the Qing dynasty rarely recorded,the 2015 edition of Chinese Pharmacopoeia and the processing norms of provinces and cities throughout the country mainly recorded fried Codonopsis Radix with rice,fried Codonopsis Radix with bran,fried Codonopsis Radix with honey,fried Codonopsis Radix,steamed Codonopsis Radix,and so on.Because the processing methods and processing norms of Codonopsis Radix are different from each other,it is difficult to establish a unified quality standard of Chinese medicinal pieces.This paper refers to the books of Codonopsis Radix collected since the Qing dynasty and the related partial literature published after 2000,which sums up and summarizes the textual research of Codonopsis Radix,the processing status of its producing area and the general situation of its processing research,and so on;it provides a powerful scientific basis for the further study on the processing in production place and processing of Codonopsis Radix,especially taking the method of frying Codonopsis Radix with rice as the breakthrough point.关键词：Codonopsis Radix;processing in production place;processing;chemical composition;pharmacological effect;processing technology2|2|6更新时间：2024-09-23
- 摘要：In recent years,the incidence and mortality of clinical fungal infections have been increasing rapidly. Invasive fungal infections,especially candidiasis,have become the major cause. Currently,side effect have been found in many existing antifungal agents to different extents. In addition,with the extensive application of broad-spectrum antifungal agents in clinic,there has been a notable increase in drug resistance. Thus,it is very important to develop new and effective antifungal agents. Natural plant compositions,especially essential oils, have many beneficial biological activities,such as antibacterial,anti-inflammatory,antitumor and analgesic activities. They had already become a hotspot in the research and development of antifungal drugs, because of their advantages, such as wide sources,low toxicity,broad spectrum and diverse functions. The antifungal activity of essential oils is closely related to their components and contents. Therefore,in this paper,the progress of research on analysis of chemical compositions and antifungal activity of essential oils from different families of plants against Candida spp. was reviewed. Extraction and analysis of essential oils from Labiatae,Lauraceae,Myrtaceae and other plants were systematically summarized. Methods and mechanisms of action of anti-Candida activity were investigated. The direction of current studies was also discussed. All of these may provide a direction for the analysis and activity of essential oils in the future and lay the foundation for developing plant essential oils into new anti-Candida agents. It may also provide new solutions for clinical infections caused by drug resistance problems.关键词：plant essential oil;Candida;antifungal;chemical composition2|2|1更新时间：2024-09-23
- 摘要：<正>《中国实验方剂学杂志》主编:我们谨此郑重通知:依据文献计量学的原理和方法,经研究人员对相关文献的检索、统计和分析,以及学科专家评审,贵刊《中国实验方剂学杂志》入编《中文核心期刊要目总览》2017年版（即第8版）之"中国医学"类的核心期刊。该书由北京大学出版社出版。书中按《中国图书馆分类法》的学科体系,列出了78个学科的核心期刊表,并逐一对核心期刊进行了著录。著录项目包括:题名、并列题名、主办单位、创刊时间、出版周期、学科分类号、ISSN号、CN号、邮发代号、编辑部地址、邮政编码、电话、网址、电子邮箱、内容简介等。3|11|0更新时间：2024-09-23
- 摘要：Myocardial ischemia and reperfusion are easy to cause a series of injuries, such as arrhythmia, cardiac function decline and myocardial necrosis, which seriously affect the therapeutic effect. Therefore, in-depth study of the pathological mechanism and the prevention and treatment measures of myocardial ischemia-reperfusion injury is the current research hotspot and difficulty. The current mechanism of myocardial ischemia-reperfusion injury focuses on myocardial energy metabolism disorders, oxidative stress, calcium overload, mitochondrial damage, inflammation, and apoptosis. These pathological processes also have complex interactions and mutual promotion relationship, which make clinical control more difficult. Modern western medicine has a limited effect due to its simple target and more adverse reactions. Traditional Chinese medicine(TCM) has many advantages, such as multiple signaling pathways and multiple targets, which have significant effects in the prevention and treatment of cardiovascular diseases. Myocardial ischemia-reperfusion injury is defined as chest apoplexyin TCM. The therapy of ‘reinforcing Qi and activating blood circulation’ is the main therapeutic principle of preventing and treating myocardial ischemia-reperfusion injury in TCM, which is widely applied in clinical practice. Experiments show that TCMs have a intervening effect on multiple pathological mechanisms of myocardial ischemia-reperfusion injury, which can significantly improve myocardial injury and reduce inflammatory infiltration of myocardial cells. Its mechanisms of action include resisting oxidative stress, inhibiting calcium overload, reducing mitochondrial damage, improving myocardial energy metabolism, resisting inflammation, and reducing apoptosis. However, most of the current researches focus on active ingredients of TCM, and there are only a few researches on the therapy of ‘reinforcing Qi and activating blood circulation’. This paper summarizes researches of this therapy on myocardial reperfusion injury, and reviews monomers, drug pairs and group prescriptions, in the expectation of providing a theoretical basis for the clinical application against myocardial ischemia-reperfusion injury.关键词：reinforcing Qi and activating blood circulation;myocardial ischemia-reperfusion injury;cardioprotection;autophagy;oxidative stress2|2|14更新时间：2024-09-23
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