最新刊期
卷 24 ， 期 4 ， 2018
- 摘要：Objective: To find out the correlation between information groups of delivery dose and aerosol particle size of Reduning inhalation solution. Method: Taking chlorogenic acid and geniposide as index components,the delivery dose uniformity was determined by a breathing simulator and the aerodynamic particle size distribution was measured by a new generation of particle impactor.Realtime particle size distribution was monitored by a laser diffractometers.The correlation analysis between information groups of delivery dose and aerosol particle size was investigated. Result: Most primitive variables of two sets had good intra-class correlations,but the inter-group correlation was not as good as intra-class correlation.After the canonical correlation analysis,two pairs of canonical variables had significant correlations with correlation coefficients being 0.998 and 0.955,respectively.Almost 90% information of delivery dose group and more than 70% information of particle size group could be elucidated by the two pairs of canonical variables. Conclusion: There is a good intra-class correlation for each of delivery dose group and particle size group,but the correlation between two sets is not so good.Canonical variables perform better inter-group correlation by comparing with original variables,indicating there is a certain correlation between delivery dose and aerosol particle size of Reduning inhalation solution.Furthermore,original variables of these two sets can be well predicted by the two pairs of canonical variables extracted.关键词：Reduning inhalation solution;in vitro inhalation performance;canonical correlation analysis;chlorogenic acid;geniposide;redundancy analysis;standardized processing4|6|2更新时间：2024-01-04
- 摘要：Objective: To develop the differences on mechanism of long-term toxicity in rats treated with raw products and processed products with wheat bran of Myristicae Semen,and to explore the connotation about its processing technology with wheat bran. Method: A urinary metabonomics method was developed to study on long-term toxicity in rats of Myristicae Semen before and after being processing,rats were divided into three groups,including normal group,the raw products group and the processed products group with the dose of 10 g·kg-1 for 12 weeks.Urinary samples of 24 hours after stopping the intragastric administration were collected.Partial least squares-discriminant analysis (PLS-DA) was employed to process data from all the groups,variable importance in the projection (VIP) were adopted to analyze potential biomarkers. Result: Good separation was observed between the raw products group and the normal group,the prcessed products group lied between the two groups,and was close to the normal group.Seventeen potential biomarkers related with long-term toxicity were screened out.Compared with those in the normal group,creatinine in the urine associated with renal toxicity significantly decreased in the raw products group;pantothenic acid,carnitine C2: 0 and amino acids in the urine associated with hepatotoxicity metabolized abnormally;these biomarkers in the processed products group were adjusted significantly to the normal group. Conclusion: When large amounts of the raw Myristicae Semen are taken for a long time,hepatic and renal toxicity will occur,but its toxic effects can decrease significantly after being processed with wheat bran.关键词：Myristicae Semen;processed products with wheat bran;creatinine;long-term toxicity;urinary metabonomics;reducing toxicity by processing;amino acids6|6|5更新时间：2024-01-04
- 摘要：Objective: To screen the specific binding components in Buyang Huanwu Tang(BYHWT) on neuron-like PC12 cells,and to provide experimental basis for further study and application of this compound. Method: A method on analysis neuron-like PC12 cells specificity binding components in BYHWT by solid-phase extraction(SPE),neuron-like PC12 cells binding assay combined with SPE was employed,and the samples were analyzed by UPLC-MS/MS,mobile phase was methanol-0.1% formic acid aqueous solution under gradient elution,the injection volume was 3 μL and the flow rate was set as 0.3 mL·min-1.The mass spectrometric conditions were set as following:atomizer pressure and auxiliary gas pressure of 379.2 kPa,air curtain gas pressure of 241.3 kPa,ion source temperature at 500 ℃,spray voltage of －4.5 kV. Result: The neuron-like PC12 cells specificity binding components in BYHWT were found as follows:6-hydroxykaempferol-3,6,7-tri-O-glucoside,6-hydroxykaempferol-3,6-di-O-glucoside and calycosin-7-O-β-D-glucoside. Conclusion: These three components may be the important compositions for neuroprotective function in BYHWT.关键词：solid-phase extraction;neuron-like PC12 cells;cell membrane solid-phase chromatography;Buyang Huanwu Tang;6-hydroxykaempferol-3,6-di-O-glucoside;calycosin-7-O-β-D-glucoside4|6|5更新时间：2024-01-04
- 摘要：Objective: To optimize the main extraction process of Xiaochuan prescription based on allergic asthma model of rats. Method: The total number of cells in bronchoalveolar lavage fluid(BALF),levels of interleukin-4(IL-4),IL-5,interferon-γ(IFN-γ),immunoglobulin E(IgE) in serum,morphological changes of lung tissue and other pharmacodynamics indexes on model rats combined with transfer rates of index components in medicinal materials were used as evaluating indicators for four extraction processes of Xiaochuan prescription. Result: The four main processes all can increase the total number of cells in BALF and reduce the role of lung inflammation and emphysema in the model rats;compared with the model group,the levels of IL-4,IFN-γ and IgE in the fourth process were significantly different(P<0.01),and the level of IL-5 was signigicantly different(P<0.05);the total transfer rate of ephedrine hydrochloride and pseudoephedrine hydrochloride was 58.97%,the total transfer rate of prim-O-glucosylcimifugin and 5-O-methylvisammioside was 63.88%, the transfer rate of schisandrin was 60.26% and transfer rate of loganin was 62.79% in the fourth process;the fourth process had the highest transfer rates of all index components. Conclusion: The fourth process group is the best by evaluating in the efficacy screening test and transfer rates of index components,and it can be used as the main extraction process of Xiaochuan prescription.关键词：Xiaochuan prescription;pharmacodynamic test;extraction process;index component;asthma;bronchoalveolar lavage fluid;interleukin8|7|0更新时间：2024-01-04
- 摘要：Objective: To explore a extracting method of genomic DNA from Ranae Oviductus and lie a foundation for further molecular experiments of this medicinal herb. Method: Sodium dodecyl sulfate(SDS) method,improved SDS method,Chelex-100 method,guanidine thiocyanate(GuSCN) method,kit method and improved kit method were used to extract genomic DNA from Ranae Oviductus.The yield and quality of genomic DNA were evaluated by agarose gel electrophoresis,ultraviolet spectrophotometry and polymerase chain reaction(PCR) amplification of primer with cytochrome C oxidase Ⅰ(COⅠ) sequence. Result: Chelex-100 method,improved SDS method,GuSCN method and improved kit method could extract genomic DNA from Ranae Oviductus,while its yields of SDS method and kit method were little.The quality and yield of genomic DNA extracted by improved SDS method were high with complex operation.The improved kit method cost too much and the yield of DNA was low.The Chelex-100 method was easy to operate and cost less time,but the quality of DNA was poor.DNA extracted by GuSCN method contained many PCR inhibitors. Conclusion: The genomic DNA extacted by Chelex-100 method,improved SDS method and improved kit method all can meet the demands of PCR amplification,suitable method can be selected to extract genomic DNA from Ranae Oviductus according to the actual conditions of the laboratory.关键词：Ranae Oviductus;genomic DNA;extraction methods;improved sodium dodecyl sulfate method;guanidine thiocyanate;Chelex-100;polymerase chain reaction5|6|2更新时间：2024-01-04
- 摘要：Objective: To breed good varieties of Desmodium styracifolium. Method: Seeds of D. styracifolium were treated with chemical mutagens, including sodium azide (NaN3), ethyl methyl sulfone (EMS) and colchicine. SRAP markers were used to conduct DNA molecular identification for the variants of D. styracifolium and the control plants. Result: There were a lot of macroscopic variations in plant height, basal diameter and branch numbers of D. styracifolium. A total of 3 285 DNA bands were amplified from 10 pairs of primers, and the percentage of polymorphic bands was 96.45%. The genetic similarity coefficients of the variants ranged from 0.326 to 0.476,which were relatively low. This indicated true genetic differences between mutants and control materials of D. styracifolium. The degree of similarity differences from high to low was as follows: V-13> V-7> V-6> V-11> V-10> V-12> V-9> V-8> V-4> V-5> V-3> V-2> V-1.The molecular level effect of NaN3 on D. styracifolium was smaller than that of EMS and colchicine. After chemical mutagenesis, D. styracifolium showed a higher degree of morphological variation than varieties from other provenance. The results indicated that the artificially induced mutation breeding could shorten the breeding period and improve the mutation rate. Conclusion: The study clearly defines the phenotypic specificity and genetic diversity of D. styracifolium germplasms, and screened out some specific resources, in order to provide the important scientific basis for breeding high-yield and high-quality varieties of D. styracifolium.关键词：Desmodium styracifolium;chemical mutation;gronomic character;SRAP5|6|0更新时间：2024-01-04
- 摘要：Objective: To screen out the potentially pathogenic endophytic fungi from 18 strains of endophytic fungi isolated from roots of Salvia miltiorrhiza, in order to explore the pathogenic mechanism of these fungi. Method: The plant-endophyte symbotic experiment was conducted to choose the potential pathogens. To determine pathogenic fungi, the changes in the activities of cell wall degrading enzymes, including cellulose [carboxymethyl cellulose enzyme(Cx), β-glucosidase(β-G)], pectinase[polygalacturonase(PG), polymethylgalacturonase(PMG)] and xylanase enzymes were measured in vitro and in vivo. Result: In vitro, all of the 9 strains could secrete cell wall degrading enzymes (CWDE), including cellulose, pectinase and xylanase. Cx and β-G activities increased over time, while PG, PMG and xylanase activities first increased and then decreased. In vivo, all of the 9 strains could secrete cell wall degrading enzymes in the roots of Salvia miltiorrhiza, but with differences among them. Roots of inoculations of 7 strains showed increase in Cx and β-G activities, roots of inoculations of 6 strains showed increase in PG activity, while roots of inoculations of 9 strains showed higher PMG and xylanase activities. Conclusion: Cellulase and pectinase and xylanase play important roles as pathogenic factors, but with differences in actions of potentially pathogenic endophytic fungi.关键词：Salvia miltiorrhiza;potentially pathogenic endophytic fungi;cell wall degrading enzyme5|6|0更新时间：2024-01-04
- 摘要：Objective: To provide basis for utilizing germplasm resources and selecting superior varieties of Phytolacca acinosa. Method: Twenty-three wild germplasm resources of P. acinosa from the main distribution areas of China were collected, and their longitude, latitudeand agronomic traits were studied. Contents of water-soluble extractives and esculentoside A were determined by hot dipping and HPLC-ELSD methods respectively. The data obtained were analyzed with DPS 6.55 and Excel 2003 software by ANOVA and UPGMA methods. Result: There were significant differencesin agronomic traits among different germplasms of P. acinosa (P<0.05). Plants from Fujian and Guangxi showed superior agronomic characters and higher earthnut production. Moreover, there were significant differences in contents of water-soluble extractives and esculentoside A (P<0.05), and plants from Shaanxi and Zhejiang were higher than others. The contents had a negative correlation with height of plant, diameter of stem, length and width of leaves, length of stipe, vertical and horizontal diameter of earthnut, production of earthnut,a positive correlation with earthnut index, latitude and altitude, and no significant correlation with leaf index and longitude. Conclusion: Plantation of P. acinosain high-latitude and high-altitude areas can enhance the accumulation of water-soluble extractives and esculentoside A. Five germplasms with superior agronomic traits and four germplasms with higher contents of water-soluble extractives and esculentoside A are selected, which could be alternative high-yield and high-quality germplasms of P. acinosa.关键词：Phytolacca acinosa;water-soluble extractive;esculentoside A;variation pattern;agronomic trait;superior germplasm4|6|2更新时间：2024-01-04
- 摘要：Objective: To study the relationship between physiological indexes, photosynthetic efficiency and yield of tetraploid Scutellaria baicalensis, in order to provide the oreticalbasis for further development and utilization of tetraploid Scutellariae series D20. Method: The physiological and biochemical indexes were detected by routine methods; photosynthetic indexes were determined using LI-6400/XT portable photosynthesis measuring system; yield measurement was made by the single-factor completely randomized block designand repeated for 3 times in total; residential area covered 6 square meters (3 m×2 m), with 10 transplanting lines in each area, line spacing of 20 cmand row spacing of 5 cm. Result: SOD and POD in tetraploid leaves of S. baicalensis and CAT enzyme activity were significantly higher by 28%, 2% and 17%than those of diploid leaves, with significant difference except for POD (P<0.05); soluble sugar content was higher by 19%than that of diploid leaves, MDA content was significantly lower than that of diploid leaves, indicating a higher antioxidant ability in tetraploid Scutellariae Radix. The field trial showed significant differences between the lengths of tetraploid and diploid S. scutellaria roots, with an average of up to 34 cm,the root diameter of tetraploid Scutellariae Radix was less than that of diploid, but with more branch roots than diploid, which were up to 8 at most,andthe single root weight and total yield were also higher than those of diploid, withthe increase of production of up to 31.4%. Conclusion: Because of chromosome doubling,tetraploid of S. baicalensis showed enhanced growth and resistance against plant diseases and pests, which the refore indirectly affected the increase of yield of medicinal herbs. The enhanced photosynthetic capacity of tetraploid S. baicalensis could increase the accumulation of dry substances of plants, which directly affected its yield. This characteristic has a special application value and higher potential for increasing yield for medicinal plants.关键词：Scutellaria baicalensis;tetraploid;physiological indicator;photosynthetic characteristic;yield5|6|0更新时间：2024-01-04
- 摘要：Objective: To study the chemical constituents of Artocarpus nigrifolius of Artocarpus genus (Moraceae). Method: Compounds were isolated and purified by the normal phase silica gel, MCI gel, Sephadex LH-20, ODS, HPLC and other column chromatography separation methods. Their structures were identified by spectrascopic methods based on physicochemical properties. Result: Twenty-six compounds, including 16 triterpenoids, 2 nortriterpenoids, 6 steroids, 1 benzaldehyde derivative and 1 amino compound, were isolated from the ethyl acetate fraction in 95% ethanol extract of Artocarpus nigrifolius. All of the above isolates were identified as 20-hydroxy-4α, 4β, 14α-trimethyl-9β, 19-cyclopregnane-3-one (1), 1-(tert-butylamino)propan-2-one (2), 24-methylenecycloartanone (3), cyclolaudenone (4), 24-methylenecycloartanol (5), 4-hydroxy-3, 5-dimethxybenzaldehyde (6), cycloeucalenol (7), cycloart-23-ene-3β, 25-diol (8), β-sitosterol (9), 3β, 24, 25-trihydroxycycloartane (10), (23E)-27-nor-3α-hydroxycycloart-23-en-25-one (11), 3β-hydroxy-22, 23, 24, 25, 26, 27-hexanordammarane-20-one (12), 12-en-3β-hydroxy-olean-11-one (13), (24S)-cycloartane-24, 25-diol-3-one (14), betulinic acid (15), lupeol (16), α-amyrin (17), (22E)-25, 26, 27-trinor-3β-hydroxycycloart-22-en-24-al (18), 3β-hydroxy-urs-11-en-13β, 28-olide (19), 11α-hydroxy-α-amyrin (20), (22E)-3β-hydroxystigmasta-5, 22-dien-7-one (21), 7-ketositosterol (22), β-amyrin (23), (24S)-methyl-5-cholestene-3β, 7α-diol (24), 7α-hydroxysitosterol (25) and 7β-hydroxysitosterol (26), respectively. Conclusion: Compound 1 is a novel cycloartane C24 nortriterpenoid, and compound 2 is reported as a naturally occurring compound for the first time. Compounds 6,8,11,15-17,20,22 and 23 are isolated from Artocarpus genus for the first time. Besides, all of the other compounds were isolated from Artocarpus genus for the first time. Triterpenoids may be the antitumor active components of A. nigrifolius, and is worth further study.关键词：Artocarpus;Artocarpus nigrifolius;chemical constituent;triterpenoid;sterol4|6|1更新时间：2024-01-04
- 摘要：Objective: To isolate and purify a polysaccharide fraction from Glycyrrhiza inflate, in order to analyze its basic chemical structure and immune activity. Method: Crude polysaccharide was extracted by the water extracting-alcohol precipitating method, and purified by DEAE cellulose-52 ion-exchange and Sepharose CL-6B gel column chromatography. Neutral homogeneous polysaccharide GiP-3 was purified from the water eluate fraction. HPGPC, IR, GC-MS and 1H-NMR were applied to analyze the basic chemical structure. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay was applied to evaluate the immune activity. Result: The molecular weight of GiP-3 was 2.1×104 Da. It was composed of arabinose, rhamnose and galactose, with the molar ratio of 1: 0.12: 18.The backbone of GiP-3 was mainly made up of 1,3-linked-α-Galp residues, and the side chains were composed of 1,5-linked-α-Araf residues and 1,2,4-linked-α-Rhap residues, and mainly linked at O-6 of α-Galp residue. The results of MTT assay showed that certain concentrations of GiP-3 could promote the proliferation of lymphocyte and RAW264.7 macrophage, with significant differences (P<0.05). Conclusion: GiP-3 is a homogeneous polysaccharide extracted from G. inflate, with an effect in enhancing immune responses.关键词：Glycyrrhiza inflate;polysaccharide;isolation and purification;structural analysis;immune activity4|6|5更新时间：2024-01-04
- 摘要：Objective: To establish a method for structural identification of the metabolites of dendrobine in the human liver microsome incubation system for the first time by UPLC/LTQ-Orbitrap-MS. Method: UPLC/LTQ-Orbitrap-MS was applied to systemically analyze the dendrobine metabolism in the liver microsomes of human.Chromatography separation was performed on a Thermo Hypersil Gold C18 column (2.1 mm×150 mm,1.9 μm) with acetonitrile-0.1% formic acid aqueous solution as mobile phase in gradient elution.Electrospray ionization (ESI) was applied and operated in positive ion mode. Result: Four major metabolites in phase Ⅰ metabolism and one major metabolites in phase Ⅱ metabolism of dendrobine marked as M-250([M+H]+ m/z 250.180 1),M-262([M+H]+ m/z 262.180 0),M-280([M+H]+ m/z 280.190 6),M-296([M+H]+ m/z 296.185 6) and M-440([M]+ m/z 440.227 8) were observed in the human liver microsome incubation system. Conclusion: This study introduces a sensitive and efficient analysis method for detecting dendrobine and its metabolites in human liver mirosomes,and it lays a foundation for the further analysis on the pharmacokinetic research of dendrobine.关键词：dendrobine;Dendrobium nobile;human liver microsomes;in vitro metabolism;dendramine;phase Ⅱ metabolism5|6|6更新时间：2024-01-04
- 摘要：Objective: To compare the similarities and differences of intestinal absorption kinetics and pharmacokinetics between baicalin magnesium salt and baicalin. Method: HPLC was employed to determine the contents of baicalin magnesium salt and baicalin in samples,and the intestinal absorption kinetics parameters,such as absorption rate constant(Ka),intestinal absorption per unit volume within one hour(Abs),apparent permeability coefficient(Papp) and pharmacokinetics parameters such as the bioavailability (AUC0-t) were figured out.That was not only clarified the behaviors of baicalin magnesium salt,but also discussed the differences of the absorption of baicalin magnesium salt and baicalin in vivo and in suit by SPSS 19.0 statistical software. Result: The Ka,Abs and Papp of baicalin magnesium salt were 16.33,1.78,15.75 times than these of baicalin with significant difference (P<0.05).However,these pharmacokinetic data showed that AUC0-t of baicalin magnesium salt had no significant differences by comparing with baicalin,as well as parameters of Cmax,Tmax and CLz. Conclusion: The intestinal absorption of baicalin magnesium salt in whole intestine tract of rats is better and quicker than baicalin.Meanwhile,pharmacokinetic characteristics of baicalin magnesium salt after oral administration are not significantly different from these of baicalin,because it can be reduced into baicalin under the action of gastric acids in the body.关键词：baicalin magnesium salt;baicalin;apparent permeability coefficient;intestinal absorption kinetics;pharmacokinetics;absorption rate constant8|8|8更新时间：2024-01-04
- 摘要：Objective: To compare the effects of artemisinin, dihydroartemisinin, artesunate and artemether on osteoclasts differentiation, and investigate relevant signaling pathways. Method: Osteoclast differentiation was induced by receptor activator of nuclear factor-κB(NK-κB) ligand (RANKL) in bone marrow-derived macrophages (BMMs). After different concentrations of artemisinin, dihydroartemisinin, artemether and artesunate were treated, tartrate resistant acid phosphatase (TRAP)-positive multinucleated cells were observed by TRAP staining. TRAP, MMP-9 and cathepsin-K (Cts-K) mRNA expressions were detected by Real-time PCR, and TRAF6 and p65 protein expressions were detected by Western blot. Result: TRAP-positive multinucleated cells were successfully induced by RANKL, and TRAP, MMP-9 and Cts-K mRNA expressions, TRAF6 and p65 protein expression were significantly increased after treatment with RANKL. Importantly, artemisinin, dihydroartemisinin, artemether and artesunate could significantly reduce the number, nuclei and diameter of osteoclasts after induction with RANKL (P<0.05, P<0.01), artemisinin obviously reduced TRAP mRNA expression (P<0.01), dihydroartemisinin, artemether and artesunate significantly down-regulated the mRNA expressions of TRAP, MMP-9 and Cts-K(P<0.01), and artemether and artesunate had a better therapeutic effect. In addition, the abnormal increase of TRAF6 and p65 proteins were obviously suppressed by artemisinin and artemisinin derivatives after induction with RANKL, and the results demonstrated that artesunate was the best one(P<0.01). Conclusion: Artemisinin and artemisinin derivatives could inhibit the effect of mouse BMMs in differentiating osteoclasts, which may be related to the down-regulation of TRAP, MMP-9 and Cts-K gene expressions, and the inhibition of TRAF6/NF-κB activation. The effect of artemether and artesunate is better than that of artemisinin and dihydroartemisininat at the same concentration.关键词：artemisinin and artemisinin derivative;osteoclast;cell differentiation;receptor activator of nuclear factor-κB ligand (RANKL)4|6|0更新时间：2024-01-04
- 摘要：Objective: To observe the anti-inflammatory effect of total flavonoids from Smilacis Glabrae Rhizoma (TFSG) on acute gouty arthritis and its mechanism. Method: Totally 90 C57BL/6 mice were randomly divided into the normal group, the model group, the colchicine group (0.65 mg·kg-1), and the TFSG group (100, 300, 500 mg·kg-1), with 15 mice in each group. The acute gouty arthritis model was induced with monosodium urate (MSU) crystals, except for the normal group, and the mice's ankle swelling degree was measured. The mice's synovium tissues were collected to detect the inflammatory mediators interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) by enzyme-linked immunosorbent assay. The protein expressions of Nod-like receptor protein 3 (NLRP3), apoptosis-associated speck-like protein (ASC), and cysteine aspartic acid protease-1 (Caspase-1) were detected by Western blot, and the mRNA expressions of NLRP3, ASC, and Caspase-1 messenger in synovium tissues were detected by Real-time PCR. Result: Compared with the normal group, mice ankle swelling degree of the model group increased significantly (P<0.01), and the levels of IL-1β, IL-6, TNF-α and the protein and mRNA expressions of NLRP3, ASC and Caspase-1 were significantly increased (P<0.01). Compared with the model group, the TFSG group could significantly reduce ankle swelling degree and IL-1β, IL-6 and TNF-α levels (P<0.01), as well as NLRP3, ASC, Caspase-1 protein and mRNA expressions(P<0.01). Conclusion: TFSG has an anti-gouty arthritis effect, and its mechanism is correlated with NLRP3 inflammasome axis.关键词：total flavonoids from Smilacis Glabrae Rhizoma;acute gouty arthritis;NLRP3 inflammasome axis5|8|24更新时间：2024-01-04
- 摘要：Objective: To study the anti-atherosclerosis (AS) mechanism of resveratrol (RES) by regulating store-operated calcium channel (SOCC) induced by transient receptor potential channel protein 1 (TRPC1) and stromal interaction molecule 1 (STIM1). Method: Atherosclerosis model was established with female C57BL/6J mice that were fed with high-fat diet after ovariectomy, and then the mice were divided into control group, model group, low-dose resveratrol group, medium-dose resveratrol group, high-dose resveratrol group(50,100,150 mg·kg-1) and estradiol (E2) group(0.3 mg·kg-1). After 16 weeks, serum was collected, and the lipid level and Ca2+ concentration were detected. The morphological changes were measured by oil red O staining. The protein expressions of TRPC1, STIM1 and endothelial nitric oxide synthase (eNOS) were detected by Western blot and Real-time PCR. The activity of eNOS was measured by enzyme-linked immunosorbent assay(ELISA). Result: Compared with model group, total cholesterol(TC), triglyceride(TG), low density lipoprotein(LDL) were decreased, while the high density lipoprotein(HDL) was increased in supplementary high-dose resveratrol or estrogen groups (P<0.05). The pathological changes in the model group were obvious compared with control group, after supplementation with RES or estrogen, the pathological changes were significantly reduced in thoracic aorta. Western blot and Real-time PCR results showed that in model group, the protein expressions of TRPC1 and STIM1 were increased, while the protein expression of eNOS was decreased (P<0.05); in supplementary high-dose resveratrol or estrogen group, these changes were reversed (P<0.05). Conclusion: RES could down-regulate TRPC1 and STIM1 gene and protein expressions, inhibit SOCC-mediated Ca2+ influx, increase eNOS expression and resist atherosclerosis.关键词：resveratrol;atherosclerosis;transient receptor potential channel protein 1;stromal interaction molecule 14|6|3更新时间：2024-01-04
- 摘要：Objective: To observe the effect of Erzhiwan on uterus coefficient, estrodiol (E2) in serum, pathological changes in tissues of uterus and vagina, and expressions of estrogen receptors (ERα and ERβ) of immature mice, in order to investigate the estrogen-like mechanism, and provide experimental evidence for the clinical application of Erzhiwan in the treatment of perimenopausal syndrome. Method: Forty female Kunming mice were randomly divided into 4 groups: normal group, estrogen receptor agonist group (EV, 0.154 mg·kg-1) and Erzhiwan (1.365, 2.73 g·kg-1) groups. Seven days later, the serum level of E2 was detected by enzyme-linked immunosorbent assay (ELISA). The pathological changes in immature mouse tissues were observed by hematoxylin-eosin(HE) staining. The expressions of ERα and ERβ were detected by Western blot. Result: Compared with normal group, Erzhiwan groups showed a significant increase in the uterus coefficient (P<0.05) and a decrease in the level of E2 (P<0.05), and the growth and development of uterus and vagina, and increase of the expressions of ERα and ERβ in vagina of immature mice, with a similar effect with EV. Conclusion: Erzhiwan plays an estrogen-like effect by up-regulating the expression of estradiol in blood and the expressions of estrogen receptors in target organ.关键词：Erzhiwan;immature mice;estrogen;uterus coefficient;estrogen receptor4|6|5更新时间：2024-01-04
- 摘要：Objective: To observe the expressions of basic fibroblast growth factor (bFGF), serine/threonine kinase (Akt) and cystein-asparate protease-9 (Caspase-9) in diabetic rat ulcer granulation tissues, in order to investigate the mechanism of Shengji Xiangpi mastic in promoting the ulcer healing in diabetic rats. Method: The ulcer model of type 2 diabetic SD rats was established, and randomly divided into normal saline group, vaseline group and Shengji Xiangpi mastic group. The ulcer model of normal rats was set up as the blank control group. The rats in Shengji Xiangpi mastic group were applied with Shengji Xiangpi mastic and covered with gauze, those in vaseline group were applied with vaseline and covered with gauze, and those in normal saline group and blank control group were treated with physiological saline. The wound healing conditions in each group were observed on 1, 3, 7, 14, 21 d after modeling, and ulcer tissues were collected. Hematoxylin-eosin (HE) staining was performed to observe the morphological changes in wound granulation tissues. Real-time PCR and Western blot were used to analyze the expression of bFGF, emzyme-linked immunosorbent assay(ELISA) was used to analyze the expression of p-Akt, and immunohistochemistry was used to analyze the expression of Caspase-9. Result: Compared with vaseline group and saline group, Shengji Xiangpi mastic significantly up-regulated bFGF expression in ulcer granulation tissues of diabetic rats, increased p-Akt content, and inhibited the expression of Caspase-9. Conclusion: Shengji Xiangpi mastic can promote ulcer healing in diabetic rats through bFGF/Akt/Caspase pathway.关键词：Shengji Xiangpi mastic;diabetic ulcer;bFGF/Akt/Caspase pathway4|6|2更新时间：2024-01-04
- 摘要：Objective: To observe the effect of Tangluoning on RNA-dependent protein kinase (PKR)-like ER kinase (PERK) pathway related protein and mRNA expressions of diabetic peripheral neuropathy (DPN) rats and Schwann cells. Method: A total of sixty SD male rats were used. Except for the blank control group, the rest SD male rats were treated with high-fat diet combined with streptozotocin (STZ, 35 mg·kg-1) to replicate the DPN rats model. The rats were randomly divided into model control group, trimethylamine oxide group, low-dose Tangluoning group and high-dose Tangluoning group. After 12 weeks of continuous administration, hematoxylin-eosin staining (HE) was adopted to observe the pathological changes in sciatic nerve under the microscope. Immunofluorescence staining was used to detect expressions of p-PERK, phosphorylated eukaryotic translation initiation factor 2α (p-eIF2a) and activating transcription factor 4 (ATF4) proteins in sciatic nerve; high-sugar-induced schwann cell model was established. The experiment was divided into 25 mmol·L-1 glucose group (control), 150 mmol·L-1 glucose group (model), 150 mmol·L-1 glucose+0.1%, 1%, 10% Tangluoning groups; they were respectively treated for 24, 48 h. Real-time PCR was used to detect PERK, nuclear factor-E2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), B-cell lymphoma 2 associated X protein (Bax) and Caspase-3 mRNA expressions. Result: Pathological changes in sciatic nerve: myelinated nerve fiber had a complete dense structure and regular shape in blank control group; severe demyelination phenomenon, irregular shape and disordered arrangement were observed in model group; whereas mild demyelination phenomenon, approximately complete structure and approximately regular shape were found in Tangluoning groups. As for integral optical density, compared with the blank control group, model group exhibited a higher level (P<0.01); compared with model group, herb group exhibited a lower level (P<0.01). As for mRNA expressions of PERK, Nrf2, HO-1, Caspase 3 and Bax in Schwann cells, compared with control group, mRNA expression of PERK, Bax and Caspase 3 in model group were significantly increased (P<0.05, P<0.01); compared with model group, mRNA expressions of PERK, Caspase-3 and Bax in Tangluoning groups were significantly decreased (P<0.05, P<0.01), and mRNA expressions of Nrf2 and HO-1 were significantly increased (P<0.05, P<0.01). As for protein expressions of p-PERK, p-eIF2a and ATF4, compared with blank control group, protein expressions of p-PERK, p-eIF2a and ATF4 in model group displayed an obvious increase (P<0.01); compared with model control group, Tangluoning groups displayed an obvious decrease (P<0.01). Conclusion: Tangluoning can alleviate the pathological damage of sciatic nerve tissues and regulate the PERK pathway, including down-regulating PERK/eIF2a/ATF4 pathway and up-regulating PERK/Nrf2/HO-1 pathway when endoplasmic reticulum stress (ERS) improves DPN.关键词：Tangluoning;diabetic peripheral neuropathy (DPN);endoplasmic reticulum stress (ERS);RNA-dependent protein kinase (PKR)-like ER kinase (PERK);Schwann cell4|6|3更新时间：2024-01-04
- 摘要：Objective: To observe the changes in Ghrelin and GHS-R in hypothalamus, gastric tissue of model rats with lung and spleen deficiency-type chronic obstructive pulmonary disease (COPD), and study the regulatory effect of the brain and intestine axis to explore its role in the rat model of COPD. Method: Totally 48 Wistar rats were randomly divided into blank control group, lung and spleen deficiency-type COPD observation group (28, 35, 42 d according to different modeling time). The rats in the control group were provided with air, intratracheally injected with the same amount of normal saline, and orally administered with the same amount of normal saline. The rats in the observation group were smoked and intratracheally instilled with lipopolysaccharide (on the 1st day, the 14th day) and gavaged with diarrhea leaves to the end of modeling. Rats were put to death at the 28th, 35th and 42th days after modeling, and their gastric tissues and hypothalamus were collected. Western blot, immunohistochemistry and Real-time PCR were used to detect protein and mRNA expressions of Ghrelin and GHS-R in rat tissues, and the dynamic expressions of Ghrelin and GHS-R were observed. Result: Compared with the blank control group, the expressions of Ghrelin and GHS-R in the hypothalamus and gastric tissue of the model group decreased gradually (P<0.05), with statistically significant differences (P<0.05). The mean optical densities of Ghrelin and GHS-R in hypothalamus and gastric tissues of the model group were significantly decreased (P<0.05), with statistically significant differences. In the model group, mRNA expressions of hypothalamus and gastric tissues were also decreased (P<0.05), with statistically significant differences. Conclusion: The expressions of Ghrelin and its receptors in hypothalamus and gastric tissues may be one of the causes of COPD complicated with malnutrition. The regulatory effect of brain stem axis plays an important role in the poor nutritional status of COPD.关键词：chronic obstructive pulmonary disease (COPD);ghrelin;GHS-R4|6|1更新时间：2024-01-04
- 摘要：Objective: To observe the effect of Qishen Yiqi dripping pill on cardiac function, collagen I (Col Ⅰ), collagen Ⅲ(Col Ⅲ), matrix metalloproteinase-9 (MMP-9), proliferating cell nuclear antigen (PCNA), α-smooth muscle actin (α-SMA) and vimentin in rats after myocardial infarction. Method: Male Wistar rats were randomly divided into five groups (n=10): sham-operation group, model group, Qishen Yiqi dripping pill (135 mg·kg-1·d-1) group (QSYQ-N), Qishen Yiqi dripping pill (270 mg·kg-1·d-1) group (QSYQ-H) and captopril group. The sham-operation group was not ligated after threading. The left anterior descending coronary artery was ligated to establish the myocardial infarction model in the remaining groups. The model was evaluated by electrocardiograph(ECG). Rats were fed for 4 weeks. After 4 weeks, the hemodynamic state of rats was recorded by physiological recorder. The immunohistological method was used to detect myocardial Col Ⅰ, Col Ⅲ and PCNA, α-SMA, Vimentin expression in rats, and the enzyme linked immunosorbent assay (ELISA) was used to detect MMP-9 concentration in rat serum. Result: Compared with the sham-operation group,+dp/dtmax, -dp/dtmax and LVSP in the model group were significantly lower, while LVEDP was significantly elevated (P<0.01). Expressions of myocardial Col Ⅰ, Col Ⅲ and PCNA, α-SMA, Vimentin in the model group were increased, and serum MMP-9 concentration was increased (P<0.01). Compared with the model group,+dp/dtmax, -dp/dtmax, LVSP in QSYQ-N, QSYQ-H and captopril groups were elevated (P<0.05, P<0.01), while LVEDP in QSYQ-H and captopril groups was lower (P<0.05, P<0.01). Expressions of myocardial Col Ⅰ, Col Ⅲ and PCNA, α-SMA, Vimentin in QSYQ-N, QSYQ-H and captopril groups were down-regulated, and serum MMP-9 concentration decreased (P<0.05, P<0.01). Conclusion: Qishen Yiqi dripping pill can improve cardiac systolic and diastolic function in rats after myocardial infarction, and its mechanism might be correlated with the inhibition of deposition of collagen, the degradation of matrix metalloproteinase and the proliferation and differentiation of fibroblasts and myofibroblasts.关键词：Qishen Yiqi dripping pill;rat;myocardial infarction;cardiac function;myocardial fibrosis5|6|5更新时间：2024-01-04
- 摘要：Objective: To study the effect of Dracaenae Cochinchinensis Resina on experimental tree shrews' myocardial ischemia reperfusion model in vitro. Method: The experimental tree shrews' myocardial ischemia reperfusion model was established by the Langendorff isolated heart perfusion system. This study contained 6 groups, namely blank group, model group, solvent control group and low-dose, medium-dose, high-dose Dracaenae Cochinchinensis Resina (0.25, 0.5, 1.0 g·L-1) groups. The hearts were collected and stored at -20 ℃ after reperfusion. Alanine aminotransferase (ALT), aspartate transaminase (AST) and lactic dehydrogenase (LDH) were measured by enzyme-labeled immunosorbent assay. Creatine Kinase MB (CK-MB) was measured by immunosuppression; Superoxide dismutase (SOD) was measured by xanthinoxidase method; malondialdehyde was measured by thiobarbital staining method; infarct size was measured by 2, 3, 5-triphenyltrazoliumchloride (TTC) method, and apoptotic rate was measured by terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) method. Result: After reperfusion with Dracaenae Cochinchinensis Resina, CK-MB, LDH, ALT, AST in perfusion fluid and tissues significantly reduced, activity of SOD increased, the myocardial infarction area and TUNEL apoptosis rate reduced. Conclusion: Reperfusion with Dracaenae Cochinchinensis Resina had effect against to myocardial ischemia-reperfusion injury by inhibiting the production of excess oxygen free radicals, meanwhile decreasing myocardial infarction area and apoptosis rate.关键词：Dracaenae Cochinchinensis Resina;myocardial ischemia-reperfusion injury;drug effect;experimental tree shrew;model in vitro6|6|2更新时间：2024-01-04
- 摘要：Objective: To study the effect of polydatin on the proliferation of MCF-7 cells, in order to further explore its underlying mechanism. Method: MCF-7 cells were treated with polydatin at different doses (0, 0.2, 0.4, 1.2, 2.0, 2.8 mg·L-1). The inhibitory rate of the cells was detected by methylthiazolydiphenyl-tetrazolium bromide (MTT) assay after treatment for 48 h. To further verify the inhibitory effect of polydatinon on the proliferation of MCF-7 cells, the cell colonies were detected by the colony formation assay after treatment with polydatin (0.2, 0.4, 1.2 mg·L-1) for 10 days. After treatment with polydatin for 48 h, the effect of polydatin (0.2, 0.4, 1.2 mg·L-1) on mRNA expressions of DNA polymerase δ catalytic subunit gene POLD1, P125, p53 and p21 were detected by Real-time PCR. Protein expressions of P125, p53 and p21 were detected by Western blot. Result: Polydatin at different doses (0.2, 0.4, 1.2, 2.0, 2.8 mg·L-1) showed an inhibitory effect on the proliferation of MCF-7 cells in a concentration-dependent manner (P<0.05, P<0.01). After administration for 10 days, polydatin (0.2, 0.4, 1.2 mg·L-1) significantly inhibited the formation of cell colonies, compared with control group (P<0.05, P<0.01). After treatment for 48 h, mRNA expressions of POLD1 and P125 were significantly lower in polydatin (0.4, 1.2 mg·L-1) groups and 5-FU group than control group, while mRNA expressions of p53 and p21 were increased significantly (P<0.05, P<0.01). After treatment for 48 h, protein expression of P125 was obviously lower in polydatin (0.4, 1.2 mg·L-1) groups and 5-FU group than control group, while protein expressions of p53 and p21 were increased significantly. The effect of polydatin on mRNA and protein expressions of PLOD1, P125, p53 and p21 in polydatin (1.2 mg·L-1) group had no difference with 5-FU group. Conclusion: Polydatin shows a significantly inhibitory effect on the proliferation of MCF-7 cells. Its underlying mechanisms may be associated with the up-regulation of p53, the inhibition of expression of POLD1 and the promotion of p21.关键词：polydatin;MCF-7;proliferation;DNA polymerase δ catalytic subunit gene 1 (POLD1);DNA polymerase δ catalytic subunit 125 (P125);gene p53 (p53);cyclin dependent kinase inhibitor p21 (p21)7|6|2更新时间：2024-01-04
- 摘要：Objective: To explore the mechanism of the total alkaloids of Gelsmium elegans (TAG) on the apoptosis of the human colonic carcinoma HT-29 cells. Method: The HT-29 cells were treated with TAG for 24 h and the cell proliferation was assessed by 3-(4,5-dimethylthiazol-zy1)-2,5-diphenyltetrazolium bromide (MTT) assay; 4,6-diamidino-2-phenylindole (DAPI) staining experiment and the flow cytometry (Annexin V-FITC/PI) experiment were used to observe the apoptosis of HT-29 cells. Reverse transcriptase polymerase chain reaction (RT-PCR) test was used to analyze the mRNA expression of B cell lymphoma-2 (Bcl-2) associated X protein (Bax) and Bcl-2 of HT-29 cells after 24 hours' treatment of the TAG. Caspase-3 expression of HT-29 cells was detected by UV Spectrophotometry. Result: In the study, it was demonstrated that the TAG had the inhibition against the proliferation of HT-29 cells. The average inhibitory effect (54.17%) was observed after the HT-29 cells were treated with 200 mg·L-1 of TAG for 24 h. The apoptotic effect of the TAG on HT-29 cells was also confirmed by the DAPI staining experiment and the flow cytometry (Annexin V-FITC/PI) experiment. RT-PCR assay showed that expression of Bcl-2 mRNA was lower in the TAG-treated group than that in control group(P<0.05), but the expression of Bax mRNA was not changed obviously. The mRNA expression level of Bax/Bcl-2 in TAG-treated group was higher than that in control group (P<0.05). Caspases-3 expression was increased with the increase of TAG concentration in UV spectrophotometry (P<0.05). Conclusion: The TAG inhibited the proliferation of HT-29 cells and induced the apoptosis of HT-29 cells, and the mechanism may be associated with activating the Bax/Bcl-2 pathway.关键词：Gelsmium elegans(TAG);human colon carcinoma HT-29 cells;cell apoptosis;B cell lymphoma-2(Bcl-2) associated X protein(Bax);Bcl-25|7|2更新时间：2024-01-04
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Effect of Yiqi Huoxue Jiedu Decoction on Proliferation of Ovarian Cancer ID-8 Cells and Angiogenesis
摘要：Objective: To analyze the inhibitory effects of Yiqi Huoxue Jiedu decoction on proliferation of ovarian epithelial carcinoma ID-8 cells in vitro, observe the effect of this method on angiogenesis of human umbilical vein endothelial cells (HUVECs), and provide experimental basis for its efficacy. Method: Cell counting kit-8 (CCK-8) was used to testify the inhibitory effect of Yiqi Huoxue Jiedu decoction on the proliferation of ovarian cancer ID-8 cells; decoction serum, regulatory T cells (Tregs) isolated by immune magnetic MACS MicroBeads, as well as vascular endothelial growth factors (VEGF) were co-cultured, and then the angiogenesis of HUVEC was observed. Result: As compared with blank control group (fetal bovine serum group), drug serum groups with concentration of 10% and 20% showed inhibitory effects on ID-8 cells, and this effect was most significant at 96 h. Serum group with concentration of 20% showed remarkable advantages against 10% group at 72 h (P<0.01) and 96 h (P<0.05). HUVECs cultivated with Treg cells exhibited a better angiogenesis process. On the other hand, the vascular network was not obvious in drug serum group, and the inhibitory effect was most significant in anti-VEGF+Yiqi Huoxue Jiedu decoction. Conclusion: Yiqi Huoxue Jiedu decoction can restrict the multiplication of ovarian epithelial carcinoma ID-8 cells, and it could intervene the angiogenesis of HUVECs, which is probably associated with the intervention on Tregs.关键词：Yiqi Huoxue Jiedu decoction;ovarian cancer;ID-8;regulatory T cells;tumor angiogenesis4|6|3更新时间：2024-01-04 - 摘要：Objective: To investigate the toxicity and toxicity reversibility of non-lethal dose of vinegar-preparing Kansui Radix on zebrafish larvae, and validate the feasibility of zebrafish model for rapid assessment of drug toxicity. Method: Samples were continuously extracted with soxhlet extractor in different polar solvents to prepare different extracts. Healthy adult zebrafish were paired for spawning. The 72 hour-post-fertilization(hpf) zebrafish larvae were chosen as models to investigate their mortality rate after 72 h treatment with different extracts of processed Kansui. The toxicity and reversibility of the most toxic extraction part was evaluated on zebrafish cardiovascular system, liver, nervous system, gastrointestinal system, kidneys and other organs and systems. Result: The order of toxicities for different extraction parts of processed Kansui Radix on zebrafish larvae was as follows: petroleum ether extract > ethyl acetate extract > dichloromethane extract > ethanol extract. In the petroleum ether extract treated group, the liver intensity of the zebrafish larvae was significantly changed with the drug concentration, and the color the liver became dark as compared with blank group (P<0.01). Minor liver injury was found in the 0.054 mg·L-1 petroleum ether extract group by hematoxylin-eosin staining, but it was significantly improved in the recovery group. The residual was decreased significantly in 0.012, 0.036, 0.054 mg·L-1 petroleum ether extract groups as compared to blank control group and 0.004 mg·L-1 petroleum ether extract group (P<0.01), and their gastrointestinal tract was promoted to empty. Other systems had no obvious toxicity characteristics, with no statistically significant differences between the groups. Conclusion: In the petroleum ether, dichloromethane, ethyl acetate and ethanol extracts of vinegar-preparing Kansui, petroleum ether extract had the greatest toxicity, mainly in hepatotoxicity and gastrointestinal irritation on zebrafish larvae. Hepatotoxicity caused liver degeneration and showed high reversibility. What's more, zebrafish as a reliable mammal model for screening acute toxic agents has good application prospects.关键词：vinegar-preparing Kansui Radix;zebrafish larvae;acute toxicity;reversibility;lethal concentration of 50%5|6|5更新时间：2024-01-04
- 摘要：Objective: To study the effect of Qinhuang mixture on the life quality and immune function of patients with non-Hodgkin lymphoma with syndrome of intermingled deficiency and excess. Method: By the Real World Study method, patients were divided into observation group and control group according to their own willing. All patients received routine chemotherapy and followed up at Ruijin Hospital during January 2015 and December 2016.Patients in observation group took Qinhuang mixture during routine chemotherapy and follow-up period, while patients in control group did not take Qinhuang mixture. The observation period was 6 months. Kanofsky score (KPS), quality of life score (EORTC-QLQ-C30), peripheral blood cell immunity, humoral immunity and immune-related cytokine levels were compared between two groups after all patients reached the end of the study. Result: A total of 222 valid cases were collected. Specifically, 74 cases were in the observation group, including 20 cases in chemotherapy and 54 cases in the follow-up period; and 148 cases were in control group, including 87 cases in the chemotherapy period and 61 cases in the follow-up period. The improvement rate of KPS score in observation group was significantly superior to that in control group (P<0.05). Scores of fatigue, constipation and diarrhea in EORTC-QLQ-C30 in observation group were lower than those in control group (P<0.05). Physical, emotional function and overall health status scores of observation group were higher than those of the control group (P<0.05). Levels of CD3+, CD4+, CD4+/CD8+ ratio, immunoglobulin G (IgG), immunoglobulin M (IgM), interleukin (IL)-2 and alexin C3 in peripheral blood of the observation group were higher than those in control group (P<0.05), whereas IL-6, IL-8 and IL-10 were lower than those in control group (P<0.05). Conclusion: Qinhuang mixture can effectively improve the quality of life and immune function of patients with non-Hodgkin lymphoma with syndrome of intermingled deficiency and excess.关键词：Qinhuang mixture;lymphoma;life quality;immune function5|6|2更新时间：2024-01-04
- 摘要：Objective: To study the clinical efficacy of modified Bazhentang combined with radiotherapy and chemotherapy in treating middle and advanced cervical cancer and investigate its effect on T cell subset, serum tumor markers and survival period. Method: The 177 patients with middle and advanced cervical cancer in our hospital from February 2009 to May 2012, were selected and divided into treatment group (89 cases) and control group (88 cases) according to the random number table. Patients in two groups were treated with radiotherapy and chemotherapy, while the patients in treatment received additional modified Bazhentang. Then the short-term efficacy, T cell subset, serum tumor markers and survival period of the two groups were compared. Result: The total effective rate was 87.64% in treatment group, better than 73.86% in control group (P<0.05). After the treatment, the levels of CD3+, CD4+ and CD4+/CD8+ were significantly increased in both groups, but the level of CD8+was significantly decreased (P<0.05). After the treatment, the levels of CD3+, CD4+ and CD4+/CD8+ in treatment group were significantly higher than those in control group, while the level of CD8+ was significantly lower than that of control group (P<0.05). The expression levels of carbohydrate antigen 125 (CA125), squamous cell carcinoma associated antigen (SCCA) and carcino-embryonic antigen (CEA) were significantly decreased in both groups after treatment, and the decrease was more obvious in treatment group (P<0.05). The 1, 2,3, 5-year survival rate was 91.01%, 55.06%, 26.97%, 13.48% respectively in treatment group, higher than 73.86%, 32.95%, 9.09%, 3.41% in control group (P<0.05). Conclusion: Modified Bazhentang can significantly improve the immunity, reduce the level of serum tumor markers and prolong the survival time of the patients, so it has positive therapeutic effect on middle and advanced cervical cancer.关键词：modified Bazhentang;middle and advanced cervical cancer;clinical efficacy;T cell subset;serum tumor markers;survival period4|6|3更新时间：2024-01-04
- 摘要：Objective: To study the clinical efficacy of Weijingtang combined with Maxing Shigan Tang and investigate its effect on T cell subset and inflammatory cytokines in treating stroke-associated pneumonia with phlegm-hot in the lung. Method: The 207 cases of patients with stroke-associated pneumonia treated in our hospital from March 2014 to March 2016, were selected and divided into two groups according to the random number table, with 104 cases in treatment group and 103 cases in control group. Patients in two groups were treated by routine western medicine, while the treatment group was also given with additive Weijingtang combined with Maxing Shigan Tang. Then the treatment efficacy and related indicators, T cell subset and inflammatory cytokines of two groups were compared. Result: The total effective rate was 94.23% in treatment group, higher than 81.55% in control group (P<0.05). The relieving time for fever and cough in treatment group was significantly shorter than that in control group (P<0.05); as compared with the conditions before treatment, both groups had improvement in body temperature, respiration, and heart rate, and the above indicators in treatment group were significantly better than those in control group after treatment (P<0.05). After the treatment, the levels of CD3+, CD4+ and CD4+/CD8+ were significantly increased in both groups, but the level of CD8+ was significantly decreased (P<0.05). As compared with the control group, the levels of CD3+, CD4+ and CD4+/CD8+ were significantly higher in the treatment group, while the level of CD8+ was significantly lower (P<0.05). As compared with the conditions before treatment, hypersensitive C-reactive protein (hr-CRP), and procalciton in (PCT) were decreased in both groups after treatment, and the levels in treatment group were significantly lower than those in control group (P<0.05). Conclusion: Weijingtang combined with Maxing Shigan Tang can significantly improve clinical efficacy and cardio-pulmonary function of stroke-associated pneumonia with phlegm-hot in the lung, strengthen the immune system and reduce the level of serum inflammatory cytokines, with better efficacy.关键词：Weijingtang;Maxing Shigan tang;stroke-associated pneumonia;T cell subset;inflammatory cytokines5|8|4更新时间：2024-01-04
- 摘要：Objective: To observe the clinical efficacy and adverse reactions of Yiqi Jiedu Xiaoai decoction and chemotherapy for advanced metastatic colorectal cancer (AMCC) with stagnated-toxin spleen deficiency syndrome. Method: A total of 62 patients with AMCC stagnated-toxin spleen deficiency syndrome in our department from January 2013 to June 2015 were selected and randomly divided into two groups. Patients in control group were treated with Sijunzi Tang combined with Irinotecan-folinic acid calcium salt hydrate-5-fluorouracil(FOLFIRI)chemotherapy, while the patients in observation group were treated with Yiqi Jiedu Xiaoai decoction combined with FOLFIRI chemotherapy. The clinical efficacy, progression-free survival, median overall survival time, tumor markers, quality of life and adverse reactions were followed up in both groups, and the difference in efficacy between two groups was compared. Result: After treatment, the disease control rate was 80.65% in observation group, higher than 54.84% in control group (P<0.05). After treatment, the median progression-free survival in observation group was 11.51 months, significantly longer than 8.77 months in control group (P<0.01), and the median overall survival in observation group (25.54 months) was also significantly longer than that in control group (20.75 months) (P<0.01). In the follow-up of 30 patients with liver metastases, the control rate was 82.25% for 15 patients in observation group, significantly higher than 46.77% in control group (P<0.05). After treatment, the levels of CEA and CA199 in observation group were significantly lower than those in control group (P<0.05,P<0.01). In addition, the improvement of quality of life in observation group was significantly better than that in control group (P<0.05), and the incidence of adverse reactions in was significantly lower than that of control group (P<0.05). There was no statistically significant difference in other indicators between two groups. Conclusion: The combination of Yiqi Jiedu Xiaoai decoction combined with FOLFIRI chemotherapy can increase the disease control rate in AMCC patients with liver metastases, prolong the survival period, reduce the adverse reactions caused by chemotherapy, reduce the blood tumor markers and improve the quality of life for patients with advanced metastatic colorectal cancer with stagnated-toxin spleen deficiency.关键词：Yiqi Jiedu Xiaoai decoction;advanced colorectal rectal cancer;cancer metastasis;Irinotecan-folinic acid calcium salt hydrate-5-fluorouracil (FOLFIRI) chemotherapy4|6|0更新时间：2024-01-04
- 摘要：Objective: To observe the effect of modified Yinchen Wuling San to malignant obstructive jaundice (MOJ) in decreasing jaundice and protecting liver after the surgery of percutaneous transhepatic cholangial drainage (PTCD), and its mechanism for antioxidant stress and inflammation. Method: One hundred and twenty-eight patients with MOJ were divided into control group (64 cases) and observation group (64 cases) by random number table. All of the patients got the surgery of PTCD. Patients in control group got sodium citrate injection for 2 mL (0.266 g)/time, 2 times/day. Chenodeoxycholic acid capsule for 0.25 g/time, 3 times/day. Compound bifendate granules for 10 g/time, 3 times/day. In addition to the therapy of control group, patients in observation group got modified Yinchen Wuling San, 1 dose/day. A course of treatment was 28 days. Before the surgery, and on the 7th and 28th day after the surgery, total bilirubin (TBIL), direct bilirubin (DBIL), alkaline phosphatase (ALP), γ-glutamyl transpeptidase (GGT), total bile acid (TBA), alanine aminotransferase (ALT) and albumin (ALB) were detected. Before and after treatment, syndrome of dampness-heat was scored, and the quality of life was evaluated based on scores of European organization for research and treatment scale (EORTC QLQ-C30) and the quality of life questionnaire of malignant obstructie jaundice (QLQ-MOJ11). And levels of malondialdehyde (MDA), superoxide dismutase (SOP), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected. Result: The good improvement rate in observation group was 75%, which was higher than 56.25% in control group (χ2=4.987, P<0.05). On the 7th and 28th day after the surgery, levels of TBIL, DBIL, TBA, ALP, GGT and ALT in observation group were lower than those in control group (P<0.01). And on the 28th day after the surgery, level of ALB was higher than that in control group (P<0.01). The scores of syndrome of dampness-heat and the total score were all lower than those in control group (P<0.01). And scores of the five functional domains of QLQ-BR30 scale, the score of the overall quality of life and level of SOD were all higher than those in control group (P<0.01). And scores of the three functional domains (Jaundice, itching and digestion) of QLQ-MOJ11 scale, marasmus and fever were all lower than those in control group (P<0.01). And levels of MDA, IL-6 and TNF-α were lower than those in control group (P<0.01). Conclusion: For patients with MOJ after surgery of PTCD, in addition to the therapy of increasing choleresis, reducing enzyme and protecting liver, modified Yinchen Wuling San can relieve jaundice and clinical effect, promote recovery of liver function, improve the quality of life, and reduce oxidative stress injury and inflammatory damage, with a better clinical effect than pure western medicine treatment.关键词：malignant obstructive jaundice;surgery of percutaneous transhepatic cholangial drainage;modified Yinchen Wuling San;antioxidant stress;inflammation;quality of life5|6|0更新时间：2024-01-04
- 摘要：Objective: To discuss the clinical effect of atomization and inhalation therapy of Yiqi Qufeng decoction on symptoms and quality of life in patients with Biqiu (allergic rhinitis, AR) and deficiency-cold of lung Qi, as well as its effects on levels of interleukin-4 (IL-4), IL-5, IL-13 and cysteinyl leukotrienes (CYs-LTs). Method: One hundred and fifty patients were randomly divided into control group (75 cases) and observation group (75 cases) by random number table. Patients in control group got mometasone furoate aqueous nasal spray, 1 spray/nostril, once for every night. Patients in observation group got atomization and inhalation therapy of Yiqi Qufeng decoction, 1 time/day, 20 min/time. The treatment course was 14 days for both groups. Before and after treatment, main symptom scores and rhinoconjunctivitis quality of life questionnaire (RQLQ) were graded. Nose resistance and levels of total IgE (TIgE), specificity (sIgE), IL-4, IL-5, IL-13 and CYs-LTs were detected. The level of eosinophils was tested after treatment. Result: The total effective rate was 91.55% in observation group. Higher than 78.57% in control group (χ2=4.683, P<0.05). After treatment, the item scores and the total sores of main symptoms such as nasal congestion, nasal itching, sneeze, runny nose, itchy eyes, foreign body sensation, red eyes and tears in observation group were lower than those in control group (P<0.01). Dimensional scores and the total score of RQLQ in observation group were all lower than those in control group after treatment(P<0.01). Nose resistance in observation group was lower than that in control group (P<0.01), and levels of TIgE, sIgE, CYs-LTs, IL-4, IL-5 and IL-13 were lower than those in control group (P<0.01). Conclusion: Atomization and inhalation therapy of Yiqi Qufeng decoction in patients with Biqiu can relieve clinical symptoms and degree of sensitization, decrease nose resistance, improve quality of life, and inhibit inflammation symptoms, and its clinical efficacy was better than that of mometasone furoate.关键词：Biqiu;allergic rhinitis;deficiency-cold of lung Qi;Yiqi Qufeng decoction;atomization and inhalation therapy;quality of life;nose resistance4|6|3更新时间：2024-01-04
- 摘要：Objective: The research carries on a basic textual research on the prescriptionsource,prescription explanationand clinical application of Bateri-7,which will provide a scientific basis for further development and utilization of this compound. Method: The methods of literature research,textual research and literature reviews were adopted. Result: Bateri-7 stemmed from The Hand Copy,which was a traditional tested prescription.The prescription was composed of Caowu leaf or buds (250 g),Hezi (250 g),Duoye Jidou(100 g),Qiancao(100 g),Heiyunxiang(100 g),Yinzhu (50 g) and Shexiang(0.5 g).The rest of prescriptionschanged in the process of the clinical application of this compound.It has the functions of killing and curing the disease,treating the diseases such as pestilence,measles,acne rash and so on.Clinical efficacy of Bateri-7 was known as mongolian antibiotics. Conclusion: Bateri-7 comes from The Hand Copy,it is a traditional prescription.It has remarkable effect on the cure of viscous diseases,such as sticky heat,sticky disease,intestinal tingling and so on,it has the potential development and utilization value.关键词：Mongolian medicine;Bateri-7;prescription explanation;research on prescriptionsource;clinical application;The Hand Copy4|6|2更新时间：2024-01-04
- 摘要：As a traditional Chinese medicinal herb, Dendrobium has significant effects in nourishing yin and generating body fluid, benefiting lung and eyes, resisting cancer and prolonging life, thus with a wide distribution and a long history of application. With the development of molecular biological technology, studies on Dendrobium medicinal resources have advanced rapidly. DNA marker technology has the characteristics of micro-scale and high velocity, specificity, accuracy and credibility, and provides a new method for studies on Dendrobium resources. The genetic diversity and genetic relationship among different species of Dendrobium were analyzed based on combined use of RAPD, ISSR, AFLP, SRAP and other molecular markers. The genetic information obtained from these studies could be used for the conservation and development of Dendrobium resources. The combined application of nuclear gene, mitochondrial gene and chloroplast gene can identify Dendrobium effectively and accurately, and provide scientific basis for the further development of specific molecular identification of traditional Chinese medicinal herbs. At the same time, the combined use of different gene sequences can be taken as molecular markers of DNA barcode of Dendrobium, which will provide new ideas and methods for the identification of Dendrobium herbs, and improve the identification speed and efficiency of Dendrobium species effectively. In this paper, molecular identification, DNA sequences, functional genes of Dendrobium genus were discussed, in order to provide the theoretical basis for the further development and application of the medicinal plants, as well as scientific basis for the effective protection and sustainable development of Dendrobium.关键词：Dendrobium;molecular marker;DNA barcoding;genetic diversity5|6|5更新时间：2024-01-04
- 摘要：Tripterygium wilfordii is an excellent Chinese medicine with significantly efficacy for the treatment of rheumatoid arthritis,systemic lupus erythematosus,nephropathy and other autoimmune diseases,but its clinical applications were confined by its serious side effects.Toxic Chinese medicine has been the research focus of processing.The latest research progress on the processing of T. wilfordii in recent 30 years was reviewed in this paper according to the data base such as CNKI,SciFinder and so on.The influence of processing on the toxicity,activity and chemical constituents of T. wilfordii was systematically summarized for its further processing research,new drug development and clinical application.关键词：Tripterygium wilfordii;processing;toxicity;activity;constituents6|6|14更新时间：2024-01-04
- 摘要：To understand the development process of Dioscoreae Rhizoma in origin, processing and clinical application by sorting out the historical origin in the development of Dioscoreae Rhizoma, and preliminarily discuss the construction of the standard system of Chinese yam (Dioscoreae Rhizoma). During the analytic process, with ‘Dioscoreae Rhizoma (yam)’, ‘historical origin’ and ‘standard system’ as the key words, the related literature on yam and traditional Chinese medicine(TCM) standardization was searched in CNKI, Wanfang and other databases. In addition, Compendium of Materia Medica, Miscellaneous Records of Famous Physicians, Pharmacopoeia of the People's Republic of China (2015 edition), Flora of China and other books were accessed to summarize the history of the development of yam and trace the origin,processing and clinical application of Chinese yam. According to the actual situation, the evolution of the origin of Chinese yam has experienced a process from dispersing to concentrating in Huaiqing, Henan, and the processing technology has also undergone great evolution. Now the processing and processing technology are also being innovated. In view of the wide application of yam in clinical and daily health care, but there is still no unified planting and production standard system, the construction of the standard system of yam is to be preliminarily explored based on the current needs of the development of TCM, mainly including the planting standard system, production and processing standard system, storage and maintenance standard system, product quality standards and other standards, aimed at promoting the standardization of yam construction, and accelerating the healthy development of Chinese medicine industry.关键词：Dioscoreae Rhizoma (yam);historical origin;standard system5|6|2更新时间：2024-01-04
- 摘要：Tripterygii Radix et Rhizoma (TWHF) shows multiple pharmacological activities including heat-clearing and detoxifying, eliminating wind and dampness, detumescence and pain relief, killing parasites and relieving itch. The modern pharmacological researches also prove that it has anti-inflammatory, analgesic, anti-tumor, immune regulation and other effects. Based on this evidence, TWHF has been applied clinically for many disorders including immune diseases, renal disease and dermatosis disease, especially for the rheumatoid arthritis. However, its severe toxic and side effects, expecially hepatotoxicity and nephrotoxicity, prevent it from being widely used in clinical practice. Therefore, the reduction of toxicity has become a hot research topic in recent years. There are multitudinous ways for detoxicity, including the traditional cleansing method, processing method, compatibility method as well as the modern preparation method, structural modification method, biological technology method and their combined use, but all of these can't solve its safety problem essentially. In the ancient medical literature, the drug toxicity often referred to the bias of this drug. In the view of the theory of traditional Chinese medicine(TCM), all of the drugs have toxicity, and the key is their correct use. The compatibility of TCM is an important part of Chinese medicine culture. From the embryonic form of compatibility in Spring and Autumn and Warring States period to the prescriptions with compatibility between monarch and ministerial drugs in Huangdi Neijin,formation of compatibility theory in Sheng Nong's Herbal Classic and spreading of classic formulations in Treatise on Febrile Diseases, the development and significance of compatibility application of TCM was recorded. With the gradual development of TCM towards modernization and internationalization, the compatibility of TCM has been further developed with quality control and clear mechanism. In this study, with TWHF detoxicity by compatibility as the center, and based on the changes of its form, the current situation and dilemma of reducing toxicity of TWHF were analyzed to provide ideas for toxicity reducing and efficacy enhancing of T. wilfordii, promote its rational development and safe clinical application, and provide a reference for the rational use of other toxic Chinese medicines.关键词：Tripterygii Radix et Rhizoma;detoxicity;compatibility;component compatibility6|7|9更新时间：2024-01-04
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