最新刊期
卷 24 ， 期 8 ， 2018
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Discussion on Whole Quality Control Mode of Traditional Chinese Medicine Based on Standard Decoction
摘要：In recent years,great progress has been made in the quality control of traditional Chinese medicine (TCM),a series of research strategies and methods have been put forward successively by scholars at home and abroad.With the extensive application of fingerprint,multi-measurement with a single standard,multi-component thin-layer chromatography in the 2015 edition of Chinese Pharmacopoeia,the framework of the whole quality control of TCM is more and more clear.In the meantime,some scholars put forward the concept of standard decoction of prepared slices of Chinese crude drugs,and they described the connotation and denotation of standard decoction initially,and summed up its preparation technology and quality control method.This paper defined the concept of standard decoction and discussed its properties and preparation methods;and illustrated its function and significance in product development,quality control,and post-marketing drug evaluation of single or classical formulas.The whole quality control mode of TCM based on standard decoction were investigated based on case analysis.The paper provides a method and reference for the whole quality control of TCM.关键词：standard decoction;traditional Chinese medicine (TCM);dispensing granules;classical formulas;whole quality control mode of TCM5|6|52更新时间：2024-01-04 - 摘要：Cardiovascular disease is an important research content of collateral theory,collateral theory takes pulse as the independent entity organ as the starting point,and considers that it has its own structure,function and metabolism characteristics and specific pathogenesis.This theory,based on the independent specificity of pulse,puts forward new treatment ideas for the multi-system vascular related diseases.Tongxinluo capsules,which is developed for the pathogenesis of collateral,can not only treat related cardiovascular diseases alone,but also show synergistic effects in the combination therapy with modern medicine.It has significant clinical effect,showing the effect of systemic intervention at various stages of cardiovascular disease (atherosclerosis,coronary heart disease angina pectoris,acute coronary syndrome/acute myocardial infarction after percutaneous coronary intervention).Tongxinluo capsules shows the effect of systemic intervention in cardiovascular diseases and provides some reference for effectively stopping the progression and development of cardiovascular continuum.关键词：Tongxinluo Capsules;atherosclerosis;angina;percutaneous coronary intervention after surgery;acute myocardial infarction;collateral theory10|6|5更新时间：2024-01-04
- 摘要：Dosage is closely related to clinical efficacy.Traditional Chinese medicine (TCM) is known as the secret of no impartment is the dose.There is a great difference between the modern clinical dose and the dose in the ancient medical books,and it has been reported many cases of good results by using overdose of drug according to the ancient medical books,and there are also reports of toxic and side effects.This paper summarizes the problems caused by the dosage standard of TCM and the disputes arising from the use of overdose.At the same time,the paper summarizes the research method of TCM dose from clinical experience,Chinese modern literature,ancient literature and modern Chinese medicine experimental research,and analyzes and prospects various methods,and expounds that the dose is the key and important problem to be solved in the inheritance and development of TCM.关键词：decoction;usual dosage;dosage standard;Chinese pharmacopoeia5|6|10更新时间：2024-01-04
- 摘要：Objective: To guide the clinical rational use of Magnoliae Officinalis Cortex by studying its usual oral dosage in decoction. Method: This paper collected ancient literature of traditional Chinese medicine (TCM) containing Magnoliae Officinalis Cortex,Excel 2013 was used to screen Magnoliae Officinalis Cortex formulae with water decoction and oral administration,database of Magnoliae Officinalis Cortex formulae was constructed by SQL Server 2008.SPSS 19.0 was adopted to calculate mode,frequency,mean and other parameters. Result: This study collected 4 674 formulae containing Magnoliae Officinalis Cortex from ancient literature of TCM,and 775 Magnoliae Officinalis Cortex formulae with water decoction,oral administration and containing dose were screened out.During these Magnoliae Officinalis Cortex formulae,dosage of Magnoliae Officinalis Cortex<3 g accounting for 23.87% of the total,3-11.2 g accounting for 52.26% of the total;11.3-30 g accounting for 12.26% of the total;>30 g accounting for 11.61% of the total. Conclusion: The commonly used dosage of Magnoliae Officinalis Cortex should be 3.5-11 g.In ancient literature,main processing method of Magnoliae Officinalis Cortex in decoction is ginger mix frying.The common dosage of Magnoliae Officinalis Cortex in formulae is relatively large before Song dynasty,and relatively less after Song dynasty.关键词：Magnoliae Officinalis Cortex;decoction;usual dose;ancient literature;processing;median;Chinese pharmacopoeia4|6|2更新时间：2024-01-04
- 摘要：Objective: To guide the clinical treatment for rational use of Asari Radix et Rhizoma by studying on its indications,formulae compatibility and usual dosage based on traditional Chinese medicine(TCM) ancient literature. Method: This paper collected ancient literatures of TCM prescription containing Asari Radix et Rhizoma,and constructed its database by SQL Server 2008.From oral and external use of Asari Radix et Rhizoma,this paper analyzed its indications,usual dosage and rules of formulae compatibility by means of statistical frequency and mean in SPSS 19.0 and SQL Server 2008 carrying out correlation analysis. Result: This study collected 4 489 formulae that containing Asari Radix et Rhizoma from ancient book of TCM.From the two aspects of oral and external use of Asari Radix et Rhizoma,this paper dug out the top 15 symptoms of indications and the top 10 herbs of formulae compatibility.The usual oral dosage of Asari Radix et Rhizoma was 1.85-13.75 g. Conclusion: There is a clear correlation between the indications and formulae compatibility of Asari Radix et Rhizoma.From the Han dynasty to Qin dynasty,the dosage of Asari Radix et Rhizoma decreased successively.The common dosage of Asari Radix et Rhizoma should be 1.85-13.75 g.According to different indications,Asari Radix et Rhizoma should be compatible with different herbs and different dosages,we should not be affected by the opinion of ‘the dosage of Asari Radix et Rhizoma should not exceed one Qian’.关键词：Asari Radix et Rhizoma;rule of syndrome-treatment;usual dose;ancient literature;prescription;compatibility;drug pairs4|6|7更新时间：2024-01-04
- 摘要：Objective: To guide the clinical rational use of Gypsum Fibrosum by study on its usual dosage in traditional Chinese medicine(TCM) decoction. Method: This paper collected TCM formulae containing Gypsum Fibrosum from ancient literatures,and constructed database of Gypsum Fibrosum by SQL Server 2008.This paper used Excel 2013 to screen Gypsum Fibrosum formulae with water decoction and oral administration,and SPSS 19.0 was adopted to calculate mode,frequency,mean,median and other parameters. Result: This study collected 3 546 formulae containing Gypsum Fibrosum from ancient literatures of TCM,and selected 672 decoction with dose of Gypsum Fibrosum.During these Gypsum Fibrosum formulae,formulae with dosage of Gypsum Fibrosum<15 g accounted for 47.92% of the total,<41.5 g accounted for 75.6% of the total,41.5-60 g accounted for 4.32% of the total. Conclusion: Raw Gypsum Fibrosum is mainly used in decoction containing Gypsum Fibrosum.The clinical dosage of Gypsum Fibrosum is relatively large before Song dynasty in ancient literatures of TCM formulae.This paper suggests that the commonly used dosage of Gypsum Fibrosum should be 7.5-41.5 g,its oversize dose should be 55-110 g.关键词：Gypsum Fibrosum;decoction;usual dosage;ancient literatures;Chinese pharmacopoeia;Gypsum Ustum4|6|2更新时间：2024-01-04
- 摘要：Objective: In order to provide a certain reference value for the follow-up research and development of harmine,harmine-loaded ethosomes were prepared and its formula was optimized. Method: Harmine-loaded ethosomes were prepared by ethanol injection method.With the volume fraction of ethanol,the amount of phospholipids and the ratio of phospholipids/cholesterol (P/C) as factors,the particle size,Zeta potential and encapsulation efficiency as evaluation indexes,central composite design-response surface methodology was used to optimize the prescription of harmine-loaded ethosomes.The preliminary stability of the ethosomes was also evaluated. Result: The best prescription for harmine-loaded ethosomes were 0.03% of harmine,1.13% of lecithin,P/C ratio of 41.55:1 and 28% of ethanol volume fraction.The prepared ethosomes were round with uniform size and well dispersed.The encapsulation efficiency,particle size and Zeta potential of harmine-loaded ethosomes were 79.0%,124.8 nm and -57.1 mV,respectively. Conclusion: The preparation process of harmine-loaded ethosomes is simple.The prescription design is reasonable with good stability,which lays the foundation for the further study on the transdermal delivery of harmine-loaded ethosomes.关键词：harmine;ethosomes;prescription;egg yolk lecithin;encapsulation efficiency;stability;particle size4|6|4更新时间：2024-01-04
- 摘要：Objective: To establish an analysis method of Shuanghuanglian preparations for determination of multiple components by HPLC chromatographic, in order to assist the peak identification, and detect the prediction accuracy and column suitability. Method: The retention time of the nine components in Shuanghuanglian preparations were determined on 28 C18 columns of different brands and types. The standard retention time (SRT) was calculated. Both chlorogenic acid (peak 1) and wogonin (peak 9) were taken as the linear calibration standards to forecast the retention time of the other seven components. Other 5 C18 columns of different brands and types were used for validation. In comparison of liner calibration with the two reference substances, baicalin was used as the reference substance for the determination of relative correction factors of other eight components. Result: Liner calibration with two reference substances is more accurate for predicting the retention time and suitable for more kinds of chromatographic columns than the relative retention time method. Further studies shall be made for the standardization and practicability. Conclusion: The method of liner calibration with two reference substances can be used to assist the chromatographic peak identification, with a broad practical prospect.关键词：Shuanghuanglian preparations;liner calibration with two reference substances;relative retention time;chromatographic peak identification4|6|8更新时间：2024-01-04
- 摘要：Objective: The stems of Entada phaseoloides have been used for the treatment of rheumatoid arthritis and traumatic injuries. In order to explore its bioactive constituents, 70% ethanol extract was studied systemically in chemistry. Method: The dried and powdered stems (9 kg) of E. phaseoloides were extracted for tree times with 70% ethanol at room temperature (3×24 h). The combined ethanol extracts were extracted consecutively with petroleum ether, EtOAc and n-BuOH. The components of n-BuOH extract were isolated and purified by normal and reverse phase silica gels, Sephadex LH-20 and MCI column chromatography and semi-preparative HPLC. And their structures were confirmed based on the physicochemical properties and spectral analyses. Result: Ten compounds were isolated from the n-BuOH fraction of 70% ethanol extract from stems of E. phaseoloides, which were identified as epigallocatechin (1), uridine (2), gallocatechin (3), catechin (4), epicatechin (5), procyanidin B3 (6), procyanidin B1 (7), eriocitrin (8), (-)-aromadendrin-3-O-β-D-glucopyranoside (9) and naringin dihydrochalcone (10). Conclusion: Compounds 2 and 6-10 were isolated from this plant for the first time.关键词：Entada genus;E.phaseoloides;chemical constituent;rheumatoid arthritis4|6|2更新时间：2024-01-04
- 摘要：Objective: To analyze and identify the main chemical constituents of Fangfeng Shaoyaotang by ultra performance liquid chromatography quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS/MS). Method: Agilent Eclipse XDB-C18 column (4.6 mm×250 mm, 5 μm) was used with 0.1% formic acid solution-acetonitrile as the mobile phase for gradient elution. The column temperature was set at 25℃; the flow rate was 1 mL · min-1; the injection volume was 5 μL; and the detection wavelength was 254 nm. Both positive and negative electrospray ionization sources were used to detect and analyze the chromatographic effluent. Result: Based on molecular ion peaks and secondary mass spectrometry, and also by considering the standard mass spectrometry information and related literature reports, 31 of 35 major peaks were finally identified, including 5 chromones, 4 pinane monoterpenoid glycosides, 15 flavonoids, which were the characteristic compounds of traditional Chinese medicine Saposhnikovia divaricata, Paeonia lactiflora and Scutellaria baicalensis respectively. Therefore, the main types of compounds in Fangfeng Shaoyaotang were determined. Conclusion: The established HPLC-Q-TOF-MS/MS method can be used to quickly analyze and identify the main chemical constituents of Fangfeng Shaoyaotang, which is of great significance to the further study of the pharmacological basis and its mechanism.关键词：Fangfeng Shaoyaotang;HPLC-Q-TOF-MS/MS;chemical constituents5|6|18更新时间：2024-01-04
- 摘要：Objective: To explore the change rules of color and chemical components of four kinds of rhizome Chinese medicinal herbs[Polygoni Multiflori Radix(PMR), Angelicae Dahuricae Radix(ADR), Notoginseng Radix et Rhizom, powder of Notoginseng Radix et Rhizoma] under two kinds of storage conditions. Method: These Chinese medicinal herbs were stored in simple warehouse and cool warehouse for 27 or 33 months. Their color was then observed under the sunlight. Their water content was determined based on the drying method. The contents of chemical components were determined by high performance liquid chromatography (HPLC) method. Result: With the increase of storage time, the color was deepened gradually and the content of 5-hydroxymethylfurfural (5-HMF) was increased in a wavy way for PMR stored in simple warehouse and cool warehouse. The content of stilbene glycoside was decreased and the content of combined anthraquinones was increased in a wavy way for PMR stored in simple warehouse. The content of stilbene glycoside was increased in a wavy way and the content of combined anthraquinones had no significant change for PMR stored in cool warehouse. The color deepened gradually and the content of imperatorin had no significant change for ADR stored in both simple warehouse and cool warehouse. The contents of ginsenosides Rg1 and Rb1 and notoginsenoside R1 as well as the color of Notoginseng Radix et R hizoma (NRR) and powder of Notoginseng Radix et Rhizoma showed no significant changes. Conclusion: The color and the contents of chemical components in PMR after 33 months and ADR, NRR and powder of NRR after 27 months storage in both simple warehouse and cool warehouse were changed slightly, but the quality of these four rhizome Chinese medicinal herbs were still controlled within the standard range.关键词：Polygoni Multiflori Radix;Angelicae Dahuricae Radix;powder of Notoginseng Radix et Rhizoma;storage;5-hydroxymethylfurfural;quality standard5|6|4更新时间：2024-01-04
- 摘要：Objective: To establish the rapid molecular detection method for pathogen of aconiti downy mildew and provide the basis for the quarantine of the A. carmichaeli seedling and safety evaluation of the cultivated environmental land. Method: Pathogen DNA was extracted by fungal DNA kit and rDNA-ITS sequence of pathogen was amplified by ITS1/ITS4 universal primers of ribosomal internal transcribed spacer (ITS) in fungi; the polymerase chain reaction (PCR) product was recycled, purified and sequenced. The measured ITS sequences were then compared with the related ITS sequences searched in GeneBank; specific sequences were obtained by using DNAMAN and specific primers were designed and screened by using Primer Premier 5.0.With methods mentioned above, the rapid PCR method for identification of pathogen of Aconiti downy mildew was established. Result: The L1A/L1B primers with high sensitivity and specificity were selected from 8 pairs of designed primers in Primer Premier 5.0.L1A/L1B primers could be used to amplify 670 bp bright bands with detection value from the pathogen DNA, and they can be also used to detect whether the pathogen of Aconiti downy mildew was present in the Aconitum carmichaeli seedling, adult plant and cultivated soil. Conclusion: The primers selected above can be used to detect the pathogen more quickly, easily and effectively, and the established method can be used for the early detection and quarantine of A. carmichaeli seedling with downy mildew.关键词：Aconitum carmichaeli;downy mildew;molecule detection;ITS;PCR4|6|0更新时间：2024-01-04
- 摘要：Objective: To determine the constituents of the volatile oil from bulbs, stems and leaves, styles and fibrous roots of Allium victorialis, so as to provide reference for studying the effective components on different parts of A. victorialis. Method: The volatile oil was extracted from 4 parts by using headspace solid phase microextraction (HS-SPME), and then analyzed by gas chromatography mass spectrometry (GC-MS). The relative content of each component was determined by chromatographic peak area normalization. Result: A total of 179 volatile compounds were isolated from the four parts of the A. victorialis, including 12 common compounds. Among them, 57 compounds, like methyl allyl disulfide, diallyl disulfide and dimethyl disulfide in bulbs, were identified, and the relative content was 98.84%. Among them, 48 compounds, like 1,3-dithiane, 2-hexenal and dimethyl disulfide in stems and leaves, were identified, and the relative content was 97.36%. Eighty-two components, such as 1,3-dithiane and dimethyl disulfide in styles, were identified, and the relative content was 92.90%. Fifty-one components, like dimethyl disulfide, 1,3-dithiane and dimethyl trisulfide, were identified in the roots, and the relative content was 85.89%. Conclusion: The components of volatile oil are mainly sulfur compounds. Bulb has the largest content of sulfur compounds, accounting for 79.75% of the total content.关键词：Allium victorialis;volatile oil;headspace solid phase micro extraction (HS-SPME);gas chromatography-mass spectrometry (GC-MS);sulfur compounds5|7|3更新时间：2024-01-04
- 摘要：Objective: To profile and identify metabolites of 2'-methoxykurarinone in rat plasma by UPLC-Q-Exactive Orbitrap-MS. Method: SD rats were orally administered 2'-methoxykurarinone and blood samples were collected through retro orbital sinus to obtain plasma.The extraction of analytes from rat plasma were achieved by solid-phase extraction method on a HyperSep Retain PEP column.The mobile phase consisted of acetonitrile and 0.1% formic acid in water.Separation was achieved on a Hypersil GOLD column.The data acquisition was carried out by electrospray ionization (ESI) with full scan and second stage mass scan in negative ion mode (spray voltage of 3 kV,capillary temperture at 320℃,m/z 100-1 000).Possible metabolites were detected and identified according to the information of chromatograph and mass spectrometry. Result: Seven phase Ⅰ metabolites and four phase Ⅱ metabolites of 2'-methoxykurarinone were detected in rat plasma.These metabolites were formed by a series of metabolic reactions,including dehydrogenation,hydroxylation,hydroxylation+dehydrogenation,hydration,sulfation conjugation,glucuronidation,and hydroxylation+glucuronidation. Conclusion: The method of UPLC-Q-Exactive Orbitrap-MS coupled with Xcalibur 4.0 can provide structural information of analytes,which provides a reliable method for the identification of metabolites of 2'-methoxykurarinone.The phase Ⅰ metabolic reaction of 2'-methoxykurarinone occurs mainly in the prenyl moiety.关键词：2'-methoxykurarinone;metabolites;metabolic pathway;plasma;solid-phase extraction method;glucuronidation;hydroxylation4|7|0更新时间：2024-01-04
- 摘要：Objective: To construct H9c2 cell line with high expression of heat shock transcription factor 1(HSF1), in order to investigate the effect of Shenxiong glucose injection (SGI) in protecting H2O2-induced H9c2 cellular oxidation injury with a high expression of HSF1. Method: The recombinant plasmid of GV142-HSF1 was constructed and transfected into H9c2 cells with FuGENE 6 transfection reagent, and G418 was used to obtain stably transfected cell line. The expressions of HSF1 and heat shock protein 70(HSP70) were detected with Real\|time RT-PCR and Western blot to confirm the establishment of H9c2 cell line with high expression of HSF1 (H9c2-HSF1). The H9c2-HSF cells were pretreated with SGI for 24 h, and then treated with H2O2(300 μmol ·L-1) for 0.5 h. After that, cell viability, contents of malondialdehyde(MDA) and reactive oxygen species(ROS), releases of lactate dehydrogenase(LDH) and creatine kinase(CK), activities of superoxide dismutase(SOD) and Glutathione peroxidase(GSH-Px), and expressions of HSF1 and HSP70 were examined. Result: The results of Real-time PCR and Western blot assays showed that the mRNA and protein expressions of HSF1 and HSP70 were significantly up-regulated (P<0.01) compared with the untransfected cells, which verified the establishment of H9c2-HSF1 cell line. Compared with H9c2 cells+H2O2 and H9c2-HSF1 cells+H2O2, corresponding administration unit showed an up-regulated expressions of HSF1 and HSP70 (P<0.01), an increased cell survival rate (P<0.01), and decreased releases of LDH and CK and MDA generation (P<0.01) after 6 h pretreatment with SGI and 0.5 h treatment with H2O2,dereased content of ROS and increased the activities of SOD and GSH-Px. Conclusion: H9c2 cell line with high expression of HSF1 was constructed successfully. It is found that high expression of HSF1 can significantly enhance the anti-oxidative damage effect of SGI; the mechanism may not be associated with the enhancement of the cell's capability of clearing ROS, but may be correlated with the up-regulation of HSP70 expression, which needs further study.关键词：heat shock transcription factor 1(HSF1);Shenxiong glucose injection;H2O2;H9c2 cell line;anti-oxidant damage4|6|2更新时间：2024-01-04
- 摘要：Objective: To observe the effect ofexternal application of Xugudan on expression of vascular endothelial growth factor(VEGF) and transforming growth factor-β (TGF-β) in rat model of tibial nonunion. Method: A total of 10 SD rats were selected as blank group, and 50 modeled SD rats were randomly divided into model group, high, middle and low dose Xugudan(150, 75, 25 mg · kg-1) groups, and transcutaneous electrical nerve stimulation (TENS) group. In 4 weeks, and 8 weeks after the intervention,X-ray detection and measurement of bone spacing were conducted; hematoxylin eosin (HE) staining was used to observe the morphological changes. Real-time PCR and enzyme linked immunosorbent assay (ELISA) were used todetect VEGF, and TGF-β changes in bone injury and serum. Result: As compared with the model group, bone spacing, VEGF and TGF-β expression levels were significantly increased in Xugudan high, middle and low dose groups as well as TENS group(P<0.01). As compared with TENS group, bone spacing, VEGF and TGF-β expression levels were significantly increased in Xugudan high dose group (P<0.01), butthe difference was not significant from Xugudan middle dose group, and the levels were significantly decreased in Xugudan low dose group (P<0.01,P<0.05). Conclusion: Xugudan can improve the local blood supply by regulating the expression of VEGF and TGF-β, induce vascular remodeling and bone remodeling, and promote the nonunion healing.关键词：Xugudan;bone nonunion;vascular endothelial growth factor (VEGF);transforming growth factor-β(TGF-β);transcutaneous electrical nerve stimulation (TENS)5|5|1更新时间：2024-01-04
- 摘要：Objective: To investigate the inhibitory effect of tormentic acid (TA) on the proliferation of rat hepatic stellate cell (HSC-T6) and transformation growth factor-β (TGF-β)/Smad signal pathway, so as to explore its anti-hepatic fibrosis mechanism. Method: Cell counting kit (CCK)-8 assay was used to observe the proliferation of HSC-T6 cell after treated with TA (0, 50, 60, 70, 80, 90, 100 μmol · L-1) for 24 h. The half inhibitory concentration (IC50) of TA was calculated, and the optimal concentration was selected. HSC-T6 cells in the logarithmic phase were collected and divided into normal control group, stimulation group, and high, middle and low-dose TA (40, 20, 10 μmol · L-1) treatment groups. Cell colony formation was observed using Giemsa staining; and cell apoptosis and cell cycle were examined by flow cytometry. The protein expression of Col-Ⅲ was detected by immunocytochemistry. Additionally, the protein expressions of Smad4 and α-SMA were detected by Western blot. Result: TA could inhibit the proliferation of HSC-T6 cells in a concentration-dependent manner, and its IC50 value was 81.7 μmol · L-1. Compared with the normal group, TA significantly inhibited the colony formation in a dose-dependent manner. Meanwhile, TA could also significantly promote HSC-T6 cell apoptosis and arrest cell cycle in G2 phase (P<0.05, P<0.01). In addition, TA obviously decreased the protein expressions of Col-Ⅲ, α-SMA and Smad4 (P<0.05). Conclusion: Tormentic acid can significantly suppress HSC-T6 cell activation. Its mechanism may be correlated with the promotion of cell apoptosis, the retardation of cell cycle and the inhibition of TGF-β/Smad pathway.关键词：tormentic acid;hepatic stallate cell;cell apoptosis;transformation growth factor-β(TGF-β)/Smad signaling pathway6|6|1更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of Yupingfeng San on the immune function of mice models with lung injury induced by smoked soot-related respirable particulate. Method: The experimental mice were placed in a special exposure box, and the smoke from the combustion of coal was introduced into the box. The PM 2.5 concentration in the box was maintained at 700-900 μg · m-3 to establish the model of lung injury. The Yupingfeng San decoction was given to the models by intragastric administration in 3 different ways:prevention, prevention+treatment, and treatment. Then the proportion of dust cells in the alveolar lavage fluid was detected; the count of CD68+ macrophages in the lung tissues was detected by immunocytochemistry; CD4+ and CD8+ cells counts and their ratio were detected by flow cytometry; IgE and IgG concentrations in serum and lung tissues were detected by enzyme-linked immunosorbent assay (ELISA). Result: The dust cells count and CD68+ macrophages count in alveolar lavage fluid of model mice were increased significantly; serum CD4+, CD8+ were decreased; the IgE level in serum was increased and the IgG level in serum was decreased; IgE level in the lung homogenate had no obvious change, while the IgG level in lung homogenate was decreased significantly in model group. After drug intervention, the count of CD4+ and CD8+ cells in the blood was increased, but there was no statistical difference between the two groups. The count of dust cells and the count of CD68+ macrophages were decreased significantly, most obvious in the prevention+treatment group. The IgE level in serum was lowest in the prevention group, and the IgG level in serum and lung homogenate was highest in the prevention+treatment group. Conclusion: Yupingfeng San can improve the cellular and humoral immune function of mice, and the effect can be maintained in toxic environment; however the effect in the toxic environment is far less than the earlier application effect.关键词：respirable particulate;Yupingfeng San;cellular immunity;humoral immunity;macrophages4|6|3更新时间：2024-01-04
- 摘要：Objective: To explore the mechanism of Danshen injection on promoting spinal cord functional recovery by observing the expression of hypoxia inducible factor (HIF)-1α and vascular endothelial growth factor(VEGF) in rats with spinal cord injury(SCI). Method: Fifty SD rats were selected and divided randomly into normal control group,model group, Danshen injection treatment group, Danshen injection+rapamycin group and methylprednisolone sodium group(n=10 in each group). The rats in normal group were fed normally, and all the rats in other groups received 1 mL · kg-1 · d-1 normal saline, Danshen injection,Danshen injection (including rapamycin 3 mg · kg-1) by intraperitoneal injection and methylprednisolone sodium solution by tail vein injection.The combine behavioral score(CBS) was used on 1, 3, 7, 14 d after SCI to evaluate the recovery of spinal nerve function in rats. All theses rats were killed on 14 d after SCI, and the expression levels of HIF-1α and VEGF were detected with immunohistochemical staining method and Western blot technique. Result: 14 d after SCI, as compared with the normal control group, the CBS and expression levels of HIF-1α and VEGF were elevated in the model group (P<0.05). As compared with the model group, the CBS was declined and the expression levels of HIF-1α and VEGF were elevated in Danshen injection treatment group (P<0.05). As compared with the Danshen injection treatment group, the CBS was elevated and the expression levels of HIF-1α and VEGF were declined in Danshen injection+rapamycin group(P<0.05). The differences of these indicators were not statistically significant between Danshen injection treatment group and methylprednisolone sodium group. Conclusion: Danshen injection could promote the recovery of nerve function after SCI by regulating the expression of HIF-1α and VEGF.关键词：spinal cord injury;Danshen injection;hypoxia inducible factor (HIF)-1α;vascular endothelial growth factor;rapamycin4|5|4更新时间：2024-01-04
- 摘要：Objective: To investigate the protective role of astragaloside (As) in the liver injury caused by iron overload. Method: The mice were randomly divided into blank control group, model group, as low-dose group, as middle-dose group and as high-dose group according to their weight. Mice were intraperitoneally injected with As once daily for 45 d. Then their serum was taken to determine the content of serum iron, total iron binding capacity (TIBC), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (T-BIL), malondialdehyde (MDA), and glutathione (GSH); pathological changes in liver tissues were examined by hematoxylin-eosin (HE) staining; the expression changes of protein nitration in liver tissues were detected by immunohistochemical staining; and the changes of the liver apoptosis were observed by TdT-mediated dUTP nick end labeling (TUNEL) staining. Result: As compared with the blank group, the levels of serum Fe, MDA, ALT, and AST in serum as well as Fe and T-BIL in liver were increased in the model group (P<0.01), while the levels of GSH and TIBC were decreased significantly (P<0.05,P<0.01). However, as compared with the model group, the levels of serum Fe, MDA, ALT and AST in serum as well as Fe and T-BIL levels in liver were significantly decreased in the As groups (P<0.05,P<0.05), while the levels of GSH and TIBC in liver were significantly decreased (P<0.05,P<0.01). HE results showed that as compared with blank group, hepatocyte hydropic degeneration, hepatocyte fatty degeneration and inflammatory cell infiltration were present in iron overload group; but these pathological damages were improved in different dose As groups. Immunohistochemical results showed that as compared with the blank group, the 3-nitrotyrosine (3-NT) level in the perivascular plasma was increased significantly in the iron overload group, but was significantly reduced in the As groups. TUNEL staining results showed that as compared with the blank group, hepatocyte apoptosis was significantly increased in model group; as compared with the model group, the hepatocyte apoptosis was significantly reduced in As groups. Conclusion: As could attenuate the liver injury induced by iron overload.关键词：astragaloside;iron overload;liver injury;apoptosis4|7|0更新时间：2024-01-04
- 摘要：Objective: To investigate the therapeutic effect of Nephrolepis cordifolia leaf decoction (NLD) on anal ulcer model in mice and explore its mechanism. Method: The anal ulcer model was prepared by applying 0.5 mL of 20% acetic acid (HAc) into the anus of mice.The 50 Kunming mice were randomly divided into normal group, model group, low, middle and high dose NLD (0.45, 0.9 and 1.8 g · kg-1) groups, 10 mice in each group. All the mice were killed on the 10th day after NLD application. Their anal and rectal tissues were collected and the mRNA expression levels of nuclear transcription factor-κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and cyclooxygenase-2 (COX-2) in the anal and rectal tissues of each group were detected by Real-time fluorescence quantitative reverse transcription polymerase chain reaction (Real-time PCR). Western blot was used to detect the protein expression levels of NF-κB, TNF-α, IL-1β and COX-2 in anal and rectal tissues of mice in each group. Morphological changes of anal and rectal tissues in mice were observed by hematoxylin-eosin (HE) staining. Result: NLD could restore the growth of mice body mass, reduce the rectoanal coefficient and down-regulate NF-κB, TNF-α, IL-1β and COX-2 mRNA expression in the anal and rectal tissues of mice (P<0.01), inhibit the activity of NF-κB, TNF-α, IL-1β and COX-2 protein expression in the anal and rectal tissues of mice (P<0.01). NLD 0.9 and 1.8 g · kg-1 could inhibit the infiltration of inflammatory cells and submucosa vasodilation and improve the corresponding inflammatory response. Conclusion: NLD has protective effect on the inflammatory response of acetic acid-induced anal ulcer in mice, and the mechanism may be related to inhibiting NF-κB mRNA and protein expression and down-regulating the gene expression of downstream inflammatory factors TNF-α, IL-1β and COX-2.关键词：Nephrolepis cordifolia leaf decoction (NLD);ulcer;nuclear factor-kappa B (NF-κB) p65;tumor necrosis factor-alpha (TNF-α);interleukin-1beta (IL-1β);cyclooxygenase-2(COX-2)5|5|3更新时间：2024-01-04
- 摘要：Objective: To observe the gene and protein expressions of human telomerase reverse transcriptase (hTERT), telomere binding factor 1 (TRF1), endonel polymerase (Tankyrase) in colorectal cancer SW480 cells treated with Si Junzitang serum, in order to explore the possible mechanism of prevention and treatment of ulcerative colorectal cancer-associated carcinogenesis (UCAC). Method: Colorectal cancer SW480 cells were in vitro cultured and intervened with blank fetal bovine serum, different concentrations of Si Junzitang containing serum and mesalazine serum. After intervention, SW480 cells were detected by Real-time PCR and Western blot for the mRNA and protein expressions of hTERT, TRF1 and Tankyrase. Result: Compared with the blank serum group, the serum levels of hTERT, TRF1 and Tankyrase were decreased in the low, medium and high-dose group and the methadiazine serum group (P<0.05). Compared with the methacillin-containing serum group, the gene expression of Tankyrase in the serum group was significantly lower than that in the serum of Si Junzitang (P<0.05), and the gene expressions of hTERT and TRF1 were significantly decreased in the high-dose group (P<0.05). Compared with the blank serum group, the serum levels of hTERT and TRF1 were decreased in Si Junzitang group, the middle and high-dose groups and the methadiazine serum group (P<0.05), the protein expression of Tankyrase was decreased in the drug group (P<0.05), the protein expression of Tankyrase in the Si Junzitang group was decreased, but with no significant difference. Compared with the methacillin-containing serum group, the protein expression of hTERT was significantly decreased in the high-dose group (P<0.05), the protein expression of TRF1 was significantly decreased in the high-dose group (P<0.05), the protein expression of Tankyrase in the serum-containing group was significantly lower than that in the low-dose group (P<0.05). Conclusion: The serum of Si Junzitang may inhibit the proliferation of cancer cells and induce the apoptosis of cancer cells by down-regulating the expressions of hTERT, TRF1 and Tankyrase.关键词：ulcerative colitis-associated carcinogenesis;Si Junzitang;human telomerase reverse transcriptase;telomere repeat binding factor 1;Tankyrase6|5|5更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of Gynura divaricata aqueous extract on foot injury in diabetic foot (DF) model rats, and explore its possible mechanism. Method: A total of 80 SD rats were included in the study. Among them, 8 rats were randomly selected as control group, while the other rats were given with high-fat and glucose feed and intraperitoneally injected with low-dose streptozotocin (STZ) to establish the rat model of diabetes; then, the model of DF was established by ligating their lower extremity vascular and making foot surgery. The model rats (n=40) were randomly divided into model group, metformin group (0.2 g · kg-1), and Gynura divaricata aqueous extract groups (16, 8,4 g · kg-1). The healing of ulcer were observed on day 1, 4, 7, 14, 21.After 28 days, the immunohistochemical method was used to observe the angiogenesis of the ischemic lower limb muscles. The content of intercellular cell adhesion molecule-1(ICAM-1), C-reactive protein(CRP),interleukin-6(IL-6), IL-1β, tumor necrosis factor-α(TNF-α), endothelin-1(ET-1) and thromboxane A2(TXA2) were determined by ELISA, and NO and nitric oxide synthase(NOS) content were determined by nitric acid reduction method. Real-time PCR and Western blot were used to determine vascular endothelial growth factor(VEGF) mRNA and protein expressions in ischemic lower limb muscles. Result: Compared with the normal group, the wound healing in the model group was delayed, while that of the G.divaricata aqueous extract group; Compared with the normal group, the microangiogenesis, serum NO and NOS levels, and VEGF mRNA and protein expressions in the model group decreased, whereas serum NO and NOS levels, and VEGF mRNA and protein expressions in the G. divaricata aqueous extract groups increased (P<0.05, P<0.01); compared with the normal group, ICAM-1, CRP, IL-6, IL-1β, TNF-α, ET-1, TXA2 levels in the model group significantly increased (P<0.01), while those in the G. divaricata aqueous extract groups decreased(P<0.05, P<0.01). Conclusion: G.divaricata aqueous extract could promote the foot wound healing of DF model rats, and its mechanism may be related to the inhibition of inflammation, the regulation of vascular endothelial function, the increase in the expression of VEGF, the promotion of the angiogenesis in ischemic tissues, and the improvement of the blood supply of the lower extremity.关键词：Gynura divaricata aqueous extract;diabetic foot (DF);inflammatory factor;endothelial dysfunction;vascular endothelial growth factor (VEGF)4|6|0更新时间：2024-01-04
- 摘要：Objective: To investigate the application of Zhihuang Zhitong granule in the treatment of gouty arthritis in rats and its effects on NACHT-LRR-PYD-containing proteins-3 inflammasome (NALP3) signaling pathway. Method: The SD rats were divided into six groups, normal group, model group (preparation by sodium urate injection into ankle joint cavity), Zhihuang Zhitong granule low dose group (1.0 g · kg-1), middle dose group (2.5 g · kg-1), high dose group (5 g · kg-1) and positive group (colchicine,3.0 g · kg-1). The levels of serum tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) were detected by enzyme-linked immunosorbent assay (ELISA); the gait and joint inflammation index at 24 h after modeling were evaluated; and the swelling degree of the naked joint was assessed before modeling, 6, 12, 24, 48 h after modeling. Hematoxylin eosin staining (HE) was used to observe the histopathological changes of ankle joint in each group; reverse transcription polymerase chain reaction (RT-PCR), Western blot and immunohistochemistry were used to detect the expression of NLRP3, IL-1β, apoptosis-associated speck-like protein (ASC) and Caspase-1 in the joint tissue. Result: Serum levels of TNF-α, IL-6 and IL-1β in model group were significantly increased in the model group as compared with the normal group (P<0.01). As compared with the model group, the levels of TNF-α, IL-6 and IL-1β were decreased in positive group, medium and high dose Zhihuang Zhitong granule groups (P<0.01). As compared with the normal group, the rats in the model group had poor gait and swelling joint (P<0.01); as compared with the model group, the poor gait and joint swelling got improved in the positive group and drug treatment groups, and the gait and joint swelling grades decreased gradually with the increase of drug concentration (P<0.05, P<0.01). As compared with the normal group, the NLRP3, IL-1β, ASC, and Caspase-1 mRNA, protein expression levels in the ankle joint tissue were increased in model group (P<0.01), and these levels were significantly decreased in positive group, middle dose and high dose Zhihuang Zhitong granule groups as compared with the model group (P<0.01). Immunohistochemistry showed that, as compared with the normal group, the positive expression rate of NLRP3, IL-1β, ASC and Caspase-1 was increased in model group; and as compared with the model group, the positive expression rate was decreased in positive group, low dose,middle dose and high dose Zhihuang Zhitong granule groups (P<0.01) in a dose-dependent manner. Conclusion: Zhihuang Zhitong granule can alleviate the swelling of joint in rats with gouty arthritis, and its mechanism may be related to the inhibition of NALP3 inflammatory signaling pathway.关键词：Zhihuang Zhitong granule;gouty arthritis;NALP3 inflammasome;joint swelling;mechanism4|6|14更新时间：2024-01-04
- 摘要：Objective: To the effect of different nourishing formulas Zuoguiwan, Youguiwan, Liuwei Dihuangwan, Jinkui Shenqiwan, Wuzi Yanzong Wan and Bazhentang on spermatogenic cell apoptosis of aging mice. Method: A total of 80 15-month-old aging mice were divided into 8 groups, aging group, Zuoguiwan group, Youguiwan group, Liuwei Dihuangwan group, Jinkui Shenqiwan group, Wuzi Yanzong Wan group, Bazhentang group and levocarnitine group, with 10 in each group. Another 10 2-month-old young mice were selected as control group. All of the drug groups were given the corresponding drugs by gavage at the dosage of 2.5 g · kg-1, and levocarnitine group was intramuscularly injected with the drug at the dosage of 45 mg · kg-1. All mice were administered once a day, for 4 weeks in a row. Twenty-four hours after the last administration, blood was collected from the mice by removing their eyes, and serum was separate to detect the contents of malondialdehyde(MDA), nitric oxide synthase(NOS), erythropoietin(EPO),superoxide dismutase(SOD), follicle stimulating hormone(FSH), leuteinizing hormone(LH) and testosterone(T). Then, the apoptosis of spermatogenic cells and the expression of Ki-67 in testis were detected in each group. Result: Compared with control group, the contents of MDA, NOS and EPO in aging group increased, with no significant difference in SOD(P<0.05). Compared with aging group, the contents of MDA, NOS and EPO in treatment groups decreased, while their SOD increased (except for Wuzi Yanzong Wan group and Liuwei Dihuangwan)(P<0.05). Compared with control group, aging group's FSN increased, while LH and T significantly decreased(P<0.05). Compared with aging group, the content of FSN significantly decreased, whereas LH and T increased(P<0.05). Based on the immunohistochemical method, compared with control group, aging group's Ki-67 expression in testis decreased, Compared with aging group, Liuwei Dihuangwan group, Wuzi Yanzong Wan group, Bazhentang group and levocarnitine group showed an increase in the expression of Ki-67 in testis(P<0.05). On the basis of Tunel method, compared with control group, the apoptosis rate of spermatogenic cells significantly increased in aging group, while that of all of the treatment groups decreased, especially in the Wuzi Yanzong Wan group(P<0.05). Conclusion: All of the six different nourishing formulas have an inhibitory effect on the apoptosis of spermatogenic cells of aging mice, with the best efficacy in Wuzi Yanzong Wan.关键词：Zuoguiwan;Youguiwan;Liuwei Dihuangwan;Jinkui Shenqiwan;Wuzi Yanzong Wan;Bazhentang;apoptosis of spermatogenic cell;aging mice6|6|2更新时间：2024-01-04
- 摘要：Objective: To study the clinical efficacy and safety of modified Wuhutang combined with azithromycin sequential therapy on anti-inflammatory, proinflammatory cytokines and respiratory function of mycoplasmal pneumoniae pneumonia children (MPP) with symptom of phlegm-heat obstructing lung. Method: Totally 135 cases MPP children treated in our hospital from March 2015 to December 2016 were selected and divided into two groups according to the admitted order, with 67 cases in clinical group and 68 cases in control group. Patients in control group were treated with azithromycin sequential therapy, while the clinical group was additionally given Wuhutang, which was decocted with clear water, one dose a day. Then the clinical efficacy, anti-inflammatory cytokines, proinflammatory cytokines and respiratory function of the two groups were compared after treatment for 14 days. Result: Compared with 85.29% in control group, the total effective rate of clinical group was 94.03%, with significant differences (P<0.05). Compared with before treatment, interleukin (IL)-4 and IL-10 of two groups decreased after treatment, while IL-2 increased (P<0.05). Compared with control group, the levels of IL-4 and IL-10 in patients of experimental group were lower after treatment, while IL-2 was higher (P<0.05). Comparison of the levels of IL-6, tumor necrosis factor (TNF)-α and IL-8 before and after treatment between the two groups showed significant differences. The levels of inflammatory factors in treatment group were lower than those in control group (P<0.05). Compared with before treatment, RR of two groups decreased after treatment, while arterial oxygen partial pressure (PaO2) and PaO2/FiO2 increased (P<0.05). Compared with control group, the respiratory rate (RR) in patients of experimental group was lower after treatment, while PaO2 and PaO2/FiO2 were higher (P<0.05). Conclusion: Modified Wuhutang is very effective in treating MPP. It can significantly improve the serum inflammatory factors, proinflammatory cytokines and respiratory function.关键词：modified Wuhutang;mycoplasma pneumonia children;anti-inflammatory factor;proinflammatory factor;respiratory function;phlegm-heat obstructing lung7|6|2更新时间：2024-01-04
- 摘要：Objective: To observe the clinical efficacy of Dizhong Qianggu capsule in the treatment of kidney and blood deficiency type postmenopausal osteoporosis and on the living quality, in order to explore its effect on the markers of bone metabolism and bone turnover. Method: Totally 120 patients in line with the diagnostic criteria for the kidney and blood deficiency type postmenopausal osteoporosis were randomly divided into two groups. The two groups were given the conventional basic therapy. The control group received alendronate sodium tablets in addition to the conventional basic therapy, while the observation group received Dizhong Qianggu capsule and alendronate sodium tablets in addition to the conventional basic therapy. After 6 months, bone mineral density (BMD), bone metabolism indexes, bone transformation index, clinical efficacy, visual analogue scale (VAS) pain index and health status were scores. And the adverse reactions were recorded. Result: Compared with before treatment, L2-4, neck and Ward BMD indexes significantly increased (P<0.05), index of bone transformation significantly elevated (P<0.05), bone transformation index bone gla protein (BGP) increased, whereas type I collagen C-telopeptide (CTX-1) decreased (P<0.05), VAS pain index descended, health status improved greatly in the control group and the observation group, with significant differences between them (P<0.05). Compared with control group, the observation group showed the increase in bone density of lumbar L2-4, bone metabolism indexes bone alkaline phosphatase (BALP), calcitonin (CT), estradiol (E2), bone metabolism BALP, CT, E2 index level and bone transformation index BGP level, and the reduction in CTX-1 level and VAS pain index, with significant differences in these indicators (P<0.05). The total effective rate of the observation group was also significantly higher than that of control group. And there were a few adverse reactions. Conclusion: Dizhong Qianggu capsule has a significant efficacy in the treatment of osteoporosis, and can effectively increase BMD of the patients, and improve bone metabolism and bone turnover status. Its possible anti-osteoporosis mechanism may be correlated with the decrease of bone turnover and the inhibition of bone resorption by directly or indirectly acting on osteoblasts and osteoclasts in a multicomponent and multi-target manner.关键词：Dizhong Qianggu capsule;postmenopausal osteoporosis;bone metabolism;bone turnover7|6|3更新时间：2024-01-04
- 摘要：Objective: To observe the clinical efficacy of Da Chengqitang, Da Chaihutang quadruple therapy of syndrome differentiation for treatment of early acute pancreatitis with gastrointestinal heat and liver and gallbladder damp-heat syndrome (AP) and its effect on the serum inflammatory factors. Method: A total of 126 cases of early AP patients were randomly divided into treatment group and control group, with 63 cases in each group. The control group was treated with conventional western medicine therapy, while the treatment group was treated with quadruple therapy of traditional Chinese medicine (TCM) syndrome differentiation in addition to the therapy of the control group (Intragastric administration of TCM decoction:Da Chengqitang for gastrointestinal heat syndrome, Da Chaihutang for syndrome of dampness-heat of liver and gallbladder; TCM enema; Da Chengqitang for TCM enema; Qingre Sanjie decoction for external application of TCM; intravenous dripping with blood circulation drug, injection with breviscapine). The course of treatment was 2 weeks. The two groups were observed for the disappearance time of abdominal pain and abdominal distension, and the recovery time of bowel sound and serum amylase (AMS), lipase (LPS), white blood cell (WBC) and C reactive protein (CRP). The changes in the modified computed tomography severity index (MCTSI) were scored before and after treatment. The content changes in serum tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-10 before and after treatment of the two groups were observed. Result: The disappearance time of abdominal pain and abdominal distension, and the recovery time of bowel sounds and serum AMS, LPS, WBC, CRP and the MCTSI score after treatment in the treatment group were better than those in the control group (P<0.05). For the patients of the two groups, the levels of serum TNF-α, IL-6 after treatment were lower than those before treatment, the level of IL-10 was higher than that before treatment, the concentration levels of serum TNF-α, IL-6 in treatment group on the third and seventh days were lower than those in control group, the level of IL-10 was higher than that of the control group (P<0.05); The concentrations of serum TNF-α, IL-6, IL-10 were basically the same on the fourteenth day in the two groups. Conclusion: Quadruple therapy of TCM syndrome differentiation can obviously improve the clinical symptoms, signs, laboratory indexes, MCTSI scores, and can reduce the concentrations of serum TNF-α and IL-6, and raise the concentration of serum IL-10.关键词：Quadruple therapy of traditional Chinese medicine syndrome differentiation;acute pancreatitis;tumor necrosis factor-α;interleukin-6;interleukin-104|6|9更新时间：2024-01-04
- 摘要：Objective: To observe the clinical efficacy of Tongbu Xinbao capsule on chronic heart failure (Qi deficiency blood stasis and water flux type), in order to investigate the mechanism of Tongbu Xinbao capsule on chronic heart failure. Method: A total of 120 patients with chronic heart failure outpatients or inpatient patients were randomly divided into treatment group and control group, with 60 cases in each group. The control group got western medicine for basic treatment; in addition to the therapy of the control group, the treatment group was also given Tongbu Xinbao capsule, 4 capsule, tid, for 4 weeks. After the treatment, the clinical efficacy of the two groups were observed; and before and after treatment, symptom score, N-terminal-proBNP (NT-proBNP), plasma renin aetivity (PRA), aldosterone (ALD), angiotensin Ⅱ (AngⅡ) and homocysteic acid (HCY) levels were observed, and the blood, urine, stool routine, liver and kidney function and other laboratory parameters were detected to determine the toxic and side effects of Tongbu Xinbao capsule. Result: Efficacy of traditional Chinese medicine(TCM) syndrome of treatment group was 91.67%, which was significantly higher than 81.67% of control group. After treatment, PRA, AngⅡ, ALD, HCY, NT-proBNP were significantly decreased than before treatment, and those in the treatment group were superior to those of the control group (P<0.05). Conclusion: Conventional western medicine combined with Tongbu Xinbao capsule showed a significant therapeutic effect, suggesting that the combined administration can better inhibit RASS activity and reduced HCYof patients with heart failure, which may be related to the inhibition of PRA, Ang11, ALD, HCY, with no toxic or side effect and a reliable efficacy.关键词：Tongbu Xinbao capsule;heart failure;rennin angiotensin aldosterone system (RAAS system);homocysteine4|6|1更新时间：2024-01-04
- 摘要：Objective: To study the clinical efficacy of Fuzheng Yiliu recipe combined with neoadjuvant chemotherapy and laparoscopic assisted radical gastrectomy in treating advanced gastric cancer. Method: Totally 106 patients with advanced gastric cancer hospitalized from January 2012 to June 2014 were selected and randomly divided into observation group and control group, with 35 cases in each group. In control group, neoadjuvant chemotherapy combined with laparoscopic assisted radical gastrectomy was provided. The neoadjuvant chemotherapy regimen was performed with FOLFOX6.The observation group was treated with Fuzheng Yiliu recipe in addition to the therapy of the control group. The clinical response rate, disease control rate, adverse reaction, Karnosfsky score, survival time, tumor recurrence and metastasis were observed and compared. The levels of CD3+, CD4+, CD8+positive T cells and NK cells (CD16+CD56+), inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-6, C-reactive protein (CRP) and heme oxygenase (HO)-1 were detected and compared between the two groups. The levels of matrix metalloproteinases (MMP)-2, MMP-9, vascular endothelial growth factor (VEGF)-A and VEGF-C in the tumor markers carcinoembryonic antigen (CEA), cancer antigen (CA724 and CA50) and recurrence and metastasis were observed. Result: The clinical response rate of observation group was significantly higher than that of control group (P <0.05). The disease control rate was slightly higher than that of control group, but with no statistically significant difference. The effective rate in observation group was 69.8%, which was significantly higher than 39.6% of control group (P<0.05). The levels of TNF-α, IL-6, CRP and HO-1 in two groups were significantly lower than those in control group (P<0.05). The levels of CD3+, CD4+, CD8+, CD4+/CD8+and NK cells in observation group were significantly higher than those before treatment (P<0.05), while the levels of CEA, CA724, CA50, MMP and VEGF were significantly reduced. The incidences of leukopenia, nausea, vomiting, mucosal response and fatigue were significantly lower in observation group than those in control group (P<0.05). Conclusion: Fuzheng Yiliu recipe combined with neoadjuvant chemotherapy and laparoscopic assisted radical gastrectomy for the treatment of advanced gastric cancer patients can be significantly effective in relieving the adverse reactions caused by chemotherapy, alleviating the stress level of patients after operation, improving the immune defense function and the quality of life of patients, inhibiting tumor recurrence and metastasis, and is worth clinical application.关键词：gastric cancer;advanced stage;Fuzheng Yiliu recipe;neoadjuvant chemotherapy5|6|5更新时间：2024-01-04
- 摘要：Objective: To explore the efficacy of Shedan Yuhou pill and gabapentin in treating postherpetic neuralgia (PHN) with Qi and blood stasis patients, in order to explore its possible mechanism. Method: A total of 114 eligible patients were randomly divided into the traditional Chinese medicine (TCM) group, the western medicine group and the TCM combined with western medicine group. The TCM group was treated with Shedan Yuhou pill; gabapentin was administered to the medicine medicine group; Shedan Yuhou pill and gabapentin were given to the TCM combined with medicine medicine group. A course of treatment was 4 weeks. The simplified McGill pain score scale (McGill), skin disease life quality index (DLQI) and TCM syndrome PHN hemostasis syndrome (TCM syndrome) were observed to estimate the efficacy of the three group before and after treatment. The serum interleukin-6 (IL-6), interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α), superoxide dismutase (SOD) were detected to compare the efficiency, recurrence rate and adverse reaction in the 3 groups. Result: The efficiency of the TCM combined with western medicine group was 96.8%, which was higher than 78.8% of the TCM group and 77.1% of the western medicine group (P<0.05). After treatment, McGill, DLQI, serum IL-6, IL-2, TNF-α, SOD of the TCM combined with western medicine group improved significantly (P<0.05) in three group. The order from low to high by the efficiency and incidence of adverse reactions was the TCM group < the TCM combined with western medicine group < the western medicine group (P<0.05). Conclusion: Shedan Yuhou pill is effective in treating PHN with Qi and blood stasis, with no significant difference from gabapentin. The incidence of adverse reactions was lower. Shedan Yuhou pill combined with gabapentin has a synergistic effect, which were better than the single use of Shedan Yuhou pill or gabapentin, with a lower recurrence rate.关键词：Shedan Yuhou pill;Qi and blood stasis;postherpetic neuralgia;gabapentin6|6|3更新时间：2024-01-04
- 摘要：Objective: To study the efficacy of Wenyang Jianpi decoction combined with moxibustion and effect on recurrent oral ulcer (ROU) and its effect on inflammatory factor and Complement C3, C4. Method: A total of 132 cases of ROU patients treated in our hospital from January 2015 to June 2016 were selected and divided into two groups according to group sequential design, with 66 cases in each group. Patients in control group were treated with compound vitamin B, vitamin C and thoracic peptide gland; in addition to this therapy, the clinical group was also given Wenyang Jianpi decoction and moxibustion. Then, the treatment effect, the times of pain disappearance and oral ulcer healing, inflammatory factor and Complement C3, C4 were compared. Result: The total effective rate of clinical group was 95.45%, which was higher than 84.85% in control group. There was a significant increase (P<0.05). The times of pain disappearance and oral ulcer healing in clinical group were obviously shorter than that in control group (P<0.05). Compared with before treatment, interleukin (IL)-2 and tumor necrosis factor-α(TNF-α) of the two groups after treatment decreased, while IL-6 increased (P<0.05). Compared with control group, the levels of IL-2 and TNF-α in patients of experimental group were lower after treatment, while IL-6 was higher (P<0.05). Compared with before treatment, Complement C3 and C4 of two groups increased after treatment (P<0.05). Compared with control group, the levels in patients of experimental group were higher (P<0.05). Conclusion: Wenyang Jianpi decoction combined with moxibustion was very effective in treating ROU. It can significantly shorten the times of pain disappearance and oral ulcer healing, improve complement C3 and C4, with an apparent anti-inflammation effect, and thus is worthy of clinical promotion and application.关键词：Wenyang Jianpi decoction;recurrent oral ulcer;spleen and stomach cold and deficiency;efficacy;inflammatory factor;complement C3,C44|5|0更新时间：2024-01-04
- 摘要：Objective: To discuss the clinical efficacy of fufang Wutou microemulsion in treating rheumatoid arthritis (RA) at active stage and its effect on levels of serum C-reactive protein (CRP), tumor necrosis factor-α(TNF-α), interleukin-1 (IL-1) and interleukin-6 (IL-6). Method: Totally 120 patients with HDD were randomly divided into control group (60 cases) and observation group (60 cases) by random number table. Both groups' patients got meloxicam tablets, 7.5 mg/time, 1 time/day. And glycosides of Baishao capsules, 2 grains/day, 3 times/day. Patients in control group were also given diclofenac diethylamine emulgel according to the area of painful place, 3 times/day. And observation group was also given fufang Wutou microemulsion according to the area of painful place, 3 times/day. And a course of treatment was 4 weeks. Before and after treatment, scores of arthralgia, tender joint, swelling, joint stiffness and difficulties in joint activity were recorded. And scores of degree of disease activity (DAS28 scale), simplified disease activity index (SDAI) and health assessment questionnaire (HAQ) were graded. Levels of erythrocyte sedimentation rate (ESR), CRP, rheumatoid factors (RF), TNF-α, IL-1 and IL-6 were detected. And the safety was evaluated. Result: ACR20 and ACR50 in observation group were 91.67% and 56.67%, which were higher than 68.33% and 36.67% in control group (P<0.05, P<0.01), with no statistical significance in ACR70 between two groups. Scores of arthralgia, tender joint, swelling, joint stiffness and difficulties in joint activity were lower than those in control group, and the disappearance rate of main symptoms was also lower than that in control group (P<0.05, P<0.01). Sores of DAS28, SDAI and HAQ and levels of ESR, CRP, RF, IL-1, IL-6 and TNF-α were lower than those in control group (P<0.01). By Ridit, there was no statistical significance in skin irritation between two groups. And no side effect caused by Chuanwu or Maqianzi was found. Conclusion: Fufang Wutou microemulsion can control clinical symptoms and disease development, relieve acute inflammatory reaction, with an obvious clinical effect and safety, but its adverse reactions shall be observed carefully.关键词：rheumatoid arthritis;active stage;inflammatory response;Fufang Wutou microemulsion;safety4|6|0更新时间：2024-01-04
- 摘要：Objective: To observe the clinical efficacy of Huangdisan grain combined with ‘Sanjiao’ acupuncture in treating treat Alzheimer disease with symptom of marrow sea deficiency and its effect on oxidative stress and inflammatory reaction. Method: Totally 106 patients were randomly divided into control group and observation group by random number table. Both groups patients got donepezil hydrochloride tablets, 5 mg/day at bed time, and the dosage increased to 10 mg/day after 4 weeks based on the symptoms. Patients in control group was given ‘Sanjiao’ acupuncture therapy, whereas patients in observation group got Huangdisan grain (4 g/time, 3 times/day) combined with ‘Sanjiao’ acupuncture. And course of treatment was 3 months. Patients' cognitive ability was evaluated by mini-mental state examination (MMSE) and Alzheimer's disease assessment scale (ADAS-cog). Activities of daily living were evaluated by activity of daily living scale (ADL), and marrow sea deficiency was also scored. Levels of superoxide dismutase (SOD), malondialdehyde (MDA), total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-PX), interleukin-1β (IL-1β), IL-6, tumor necrosis factor-α (TNF-α) and interferon-γ(IFN-γ) were detected. Result: After Ridit analysis, the clinical efficacy of the observation group and the curative effect of traditional Chinese medicine(TCM) syndrome were better than that of the control group(P<0.05). The total effective rate of TCM in observation group was 93.88%, which was higher than 79.17% in control group (χ2=4.521, P<0.05). After treatment, score of MMSE was higher than that in control group (P<0.01), and scores of ADAS-cog, ADL and marrow sea deficiency were lower than those in control group (P<0.01). Levels of IL-1β, IL-6, TNF-α and IFN-γ were lower than those in control group (P<0.01). Conclusion: Huangdisan grain combined with ‘Sanjiao’ acupuncture can improve the cognitive ability of patients and their activities of daily living, and resist oxidant stress and inflammation.4|6|3更新时间：2024-01-04
- 摘要：Objective: A multi-target network and mechanism analysis of the main effective components of Tongxie Yaofang was carried out to illustrate the multi-component, multi-target and multi-pathway pharmacological mechanism of Tongxie Yaofang in treating irritable bowel syndrome. Method: Genes associated with irritable bowel syndrome were screened out through databases. Literatures and databases were retrieved to obtain the components and targets of Tongxie Yaofang. Functional annotation and pathway enrichment analysis of target genes were carried out by the Database for Annotation, Visualization and Integrated Discovery (DAVID) and Kyoto Encyclopedia of Genes and Genomes(KEGG) databases. The components, targets and disease pathways-related networks of Tongxie Yaofang were constructed and analyzed by Cytoscape software. Result: A total of 25 main active components of Tongxie Yaofang were screened out, involving 37 related targets. Based on this, the target component-effective target (C-T) network and the component-target-pathway (C-T-P) network were constructed, including 12 important components and 11 core targets and 26 crucial pathways. The functions of target genes involved gastrointestinal vascular smooth muscle, endocrine regulation and nerve immunity. Conclusion: Various effective components of Tongxie Yaofang act on multiple targets network, and then have a synergistic effect by multiple channels, including regulating inflammation, intestinal sensory function regulation, immune response, psychological adjustment and intestinal infection and flora disorder.关键词：Tongxie Yaofang;irritable bowel syndrome;multi-target network;mechanism analysis;pathway classification;network pharmacology5|6|7更新时间：2024-01-04
- 摘要：Objective: To predict and explore the target and mechanism of Huangqi Guizhi Wuwutang in the treatment of diabetic peripheral neuropathy (DPN) through systemic pharmacology. Method: The active constituents and related target proteins of Huangqi Guizhi Wuwutang were searched through TCMSP database. Cytoscape 3.5.1 was used to construct the topology analysis for the active ingredient-target interaction network of Huangqi Guizhi Wuwutang; STRING online database was used for the protein-protein interaction (PPI) network construction and analysis; target protein gene name was obtained from Uniprot database, David database was used for the analysis of KEGG pathway enrichment and the study for the mechanism and targets of Huangqi Guizhi Wuwutang in Treating DPN. Result: Quercetin, kaempferol, beta-sitosterol, 7-O-methylisomucronulatol and isorhamnetin were the core compounds of Huangqi Guizhi Wuwutang. The core targets included Prostaglandin G/H synthase 2, Heat shock protein HSP 90, Nuclear receptor coactivator 2; PPI network diagram showed that the key components of the active ingredients of Huangqi Guizhi Wuwutang mainly included JUN, Tumor protein p53(TP53), c-fos(FOS), mitogenactivated protein kinase 1(MAPK1), transcription factor p65(RELA), interleukin-8(IL-8); KEGG results showed that the main effects of Huangqi Guizhi Wuwutang were correlated with TNF signaling pathway, Toll-like receptor signaling pathway and other signaling pathways. Conclusion: Huangqi Guizhi Wuwutang can exert the efficacy through a variety of channels, with multiple signaling pathways acting on a variety of targets. It can be effectively predicted and analyzed by systematic pharmacology through a variety of databases and statistical analysis software, so as to show its potential effect and mechanism of action.9|7|4更新时间：2024-01-04
- 摘要：Objective: To elucidate the names of traditional Chinese medicine (TCM) of Astragali Radix through the textual research for the literal meaning of Astragali Radix. Method: Dictionaries of the Han dynasty to the Ming and Qing dynasties and ancient Chinese herbal medicine books were retrieved for the textual research of the meaning and origin of name of Astragali Radix. Result: Old radical of Qi refers to the names of Chinese herbal medicine Astragali Radix in the ancient TCM books,while grass radical of Qi refers to the TCM names of Astragali Radix in the modern books. Grass radical of Qi was interpreted as Qimu, and is now interpreted as TCM Anemarrhenae Rhizoma but not TCM Astragali Radix in Shuowen Jiezi, Yupian, Guangyun, Jiyun, Leipian and Zihui. In the Ming dynasty dictionary Zhengzitong, grass radical of Qi was interpreted as TCM Astragali Radix. Grass radical of Qi was interpreted as TCM Astragali Radix and Anemarrhenae Rhizoma in Kangxi Zidian and Hanyu Dacidian. LI Zhong-li of the Ming dynasty specially pointed out that it was wrong to write old radical of Qi as grass radical of Qi in his book of Zhengzitong. It suggested that the glyph of grass radical of Qi started to be used in the Ming dynasty. Old radical of Qi was interpreted as long in all of the previous dictionaries, and expressed its botanical morphological characteristic. Therefore, the name of old radical of Qi is consistent with the naming habit of Chinese herbal medicine. Conclusion: The herbal contents of ancient documents and Chinese herbal medicine books are compared to illuminate the literal meaning, distinguish clearly the relationship between the literal meaning and the TCM names, find out the reasons for changing the name of Astragali Radix, and provide the reference value for consulting ancient documents in the study of Chinese herbal medicine books.关键词：Astragali Radix;name of traditional Chinese medicine;textual research;grass radical of Qi;old radical of Qi4|10|1更新时间：2024-01-04
- 摘要：Diabetes is a chronic disease caused by disorder of glucose metabolism, and it has affected 9% of the total number of adults in the world. Long course and lack of efficient drugs make diabetes one of the severest diseases that bother the public health system. Developing a safe and effective hypoglycemic drug is the key to solve this problem. Traditional Chinese medicines (TCMs) and their extracts have unique advantages in treatment of diabetes, highly effective and with little side-effects. Clinical and experimental studies unveiled that oleanolic acid from TCM played the major role in its hypoglycemic effects. More and more researches have been published in these years to reveal the mechanism of oleanolic acid in diabetic treatment. At first, the pharmacological functions of oleanolic acid in treatment of diabetes and its complications were summarized in this paper. Subsequently, possible molecular mechanism of how oleanolic acid could modulate glycogenesis and glycogenolysis, promote insulin production, and enhance insulin effects were discussed, while the possible cellular pathways of oleanolic acid to improve the complications of diabetes by reducing the intracellular inflammatory factors and peroxy free radicals were investigated in this paper. Finally, the existing problems of oleanolic acid as an antidiabetic drug were discussed, and by considering the current research results, solutions were put forward, and prospects were given in terms of how to further develop, produce and promote oleanolic acid as a clinical drug for diabetes.关键词：oleanolicacid;diabetes;triterpenoid;clinical drug10|6|3更新时间：2024-01-04
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