最新刊期
卷 24 ， 期 9 ， 2018
- 摘要：Objective: To prepare and characterize poly(lactic-co-glycolic acid) (PLGA)-crebanine nanoparticles (PLGA-Cre-NPs),and evaluate its in vitro release. Method: PLGA-Cre-NPs was prepared by emulsion-solvent diffusion method with PLGA as carrier material.Central composite design-response surface methodology was applied to optimize the preparation procedure of the nanoparticles based on the evaluation indexes of encapsulation rate,particle size and polydispersity index (PDI).The in vitro release kinetics of the nanoparticles was investigated by dynamic dialysis system. Result: The optimized preparation procedure was as follows:volume of organic phase-aqueous phase of 3:10,acetone-ethanol (8:2),PLGA amount of 90 mg.The encapsulation rate of PLGA-Cre-NPs was (84.69±2.54)% with the particle size of (155.3±14.2) nm and PDI of 0.095±0.018,and it showed a regular spherical structure by scanning electron microscopy.Meanwhile,the release profile of PLGA-Cre-NPs could be well described by two phase dynamic equation,0-24 h was closer to Weibull equation,24-168 h was closer to Higuchi equation,and its half life (t1/2) was 18.06 h,the cumulative release rate was 78.77% at 168 h. Conclusion: PLGA-Cre-NPs has high encapsulation rate and uniform size,which will be expected to become a sustained-release preparation.关键词：crebanine;poly (lactic-co-glycolic acid);nanoparticles;central composite design-response surface methodology;sustained-release preparation;in vitro release;emulsion-solvent diffusion method4|6|2更新时间：2024-01-04
- 摘要：Objective: To study on the intestinal absorption characteristics of Eucommiae Cortex extract in normal rats and spontaneously hypertensive rats. Method: Everted intestinal sac model was utilized.Intestinal sac fluid samples at different time after administration of 10.0 g · L-1 of Eucommiae Cortex extract were collected and the contents of geniposidic acid,protocatechuic acid,neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,pinoresinol diglucoside and (+)-piresil-4-O-β-D-glucopyraside in intestine samples were determined by UPLC-MS/MS.Accumulative absorption (Q) and absorption rate constants (Ka) of these seven compenents were calculated,while absorbance of indicator components in intestinal absorbent liquid between normal rats and spontaneously hypertensive rats (SHR) was compared. Result: The intestinal absorption of 7 components in Eucommiae Cortex extract was linear absorption with R2 >0.99,the main absorption area was in the small intestine,and the absorption of duodenum was the best under normal conditions.Protocatechuic acid and pinoresinol diglucoside had better absorption in the jejunum under hypertensive conditions,which suggested that pathological conditions may alter the specific site of drug absorption.Compared with normal rats,the intestinal absorption of the 7 index components in spontaneously hypertensive rats model showed different degrees of difference.The absorption of geniposidic acid in the intestinal segments of spontaneously hypertensive rats model was weaker than that of normal rats,but the other components represented by pinoresinol diglucoside showed no a consistent absorption trend. Conclusion: Spontaneous hypertension can affect the intestinal absorption of Eucommiae Cortex extract,these differences can not be fully explained from the perspective of intestinal mucosal permeability reduction and back shift of absorption site,which may be related to the presence of intestinal enzymes and transporters,and the specific mechanism needs further study.关键词：Eucommiae Cortex;everted gut sac model;spontaneously hypertensive rats;geniposidic acid;pinoresinol diglucoside5|8|7更新时间：2024-01-04
- 摘要：Objective: Taking primary rat hepatocytes (PRH) model,to explore the effect of Fuzheng Huayu recipe (FZHY) on rat cytochrome P450 enzyme (CYP450). Method: PRH were isolated and cultured by two-step perfusion method.With the formation of the hepatic plate structure,FZHY was added to culture for 24 h,then replaced with culture medium containing specific probe substrates of CYP450 (phenacetin-CYP1A2,tolbutamide-CYP2C9,dextromethorphan hydrobromide-CYP2D6,testosterone-CYP3A4),the supernatant was aspirated after 2 h,the corresponding products of the probe drugs were detected by UPLC-MS/MS. Result: CYP1A2,CYP2C9,CYP2D6 and CYP3A4 in PRH had good metabolic activity.The probe drugs had no toxicity on PRH in the range of 0-500 μmol · L-1,FZHY had no toxicity on PRH in the range of 0.5-500 μg · L-1.FZHY could slightly protect PRH with concentration of 5 μg · L-1.After PRH was cultured with 5 μg · L-1 FZHY for 24 h,Michaelis constant (Km) of tolbutamide decreased by 20.8%,Km of dextromethorphan hydrobromide decreased by 39.2%,Km of phenacetin decreased by 17.4%,Km of testosterone decreased significantly. Conclusion: FZHY can inhibit the activity of CYP1A2,CYP2C9,CYP2D6 and CYP3A4 in PRH.关键词：Fuzheng Huayu Recipe;cytochrome P450 enzyme;primary hepatocytes;compound Chinese medicine;phenacetin;tolbutamide;dextromethorphan hydrobromide;testosterone6|6|3更新时间：2024-01-04
- 摘要：Objective: To screen the optimal formula for artemether oral microemulsion-in situ gel and evaluate its properties. Method: With liquidity and gelation capacity as indexes,the water phase was determined.The composition was screened by solubility assay,compatibility test,stability and adhesiveness of microemulsion-in situ gel system.Pseudoternary phase diagram was used to optimize the formula.The morphology,particle size,Zeta potential,viscosity,stability,gelation and gastric retention time in vivo of artemether microemulsion-in situ gel were investigated,respectively. Result: The optimal formula was as follows:artemether:glyceryl triacetate:cremophor EL35:transcutol HP:(0.3% gellan gum-0.1% sodium alginate with low viscosity)=0.5:4.5:5:5:85,the concentration of artemether in the microemulsion-in situ gel about 5 g · L-1. Artemether microemulsion-in situ gel in transmission electron microscope consisted of small spherical droplets, mean particle size,polydispersity index (PDI),Zeta potential,viscosity of the drug delivery system were 20.90 nm, 0.172,-19.5 mV and 15.32 mPa · s,respectively.After oral administration,it gelled in the stomach of rats immediately;six hours later,gel could still be observed and artemether could be detected in the stomach. Conclusion: The solubitily of artemether is significantly increased in microemulsion-in situ gel.The artemether microemulsion-in situ gel has characteristics of good gelation in stomach and suitable viscosity,it not only can prolong the gastric retention time of artemether but also is suitable for oral administration.关键词：artemether;microemulsion;in situ gel;oral administration;pseudoternary phase diagram;gastric retention time;gellan gum4|6|1更新时间：2024-01-04
- 摘要：Objective: In order to guarantee the seed base of Scutellariae Radix is accurate,an internal transcribed spacer 2 (ITS2) barcode reference library of Scutellariae Radix has been constructed and then a species identification method of Scutellariae Radix seeds based on DNA barcoding technology has been established. Method: ITS2 sequences were obtained from Scutellariae radix reference medicinal materials,original plant,crude medicinal materials,public database,and its substitutes.Totally 62 Scutellariae Radix seed specimens were collected from markets.For each Scutellariae Radix seed specimen,3-4 ITS2 sequences were obtained and then used for species assignment by BLAST method,genetic distance method and neighbor-joining phylogenetic tree method. Result: In total,101 ITS2 sequences of Scutellariae Radix and its substitutes have been obtained.The data analysis result showed that the intraspecific distance of Scutellariae Radix was less than its interspecific distance.The three species of Scutellaria baicalensis,S. rehderiana and S. viscidula were showed separated branch in the neighbor-joining tree.Totally 195 ITS2 sequences have been obtained from Scutellariae Radix seeds,the identification results showed that 193 sequences were Scutellariae Radix and 2 sequences were fungi contaminations. Conclusion: The ITS2 barcode database of Scutellariae Radix is stable and reliable,it can satisfy the demand of DNA barcoding analysis of Scutellariae Radix seeds.关键词：Scutellariae Radix;seeds;DNA barcoding;internal transcribed spacer;identification;Scutellaria rehderiana;original plant4|6|12更新时间：2024-01-04
- 摘要：Objective: To study on the relationship between saponins and ecological factors of different habitats of Paris polyphylla var.chinensis. Method: The saponins ingredient of P.polyphylla var.chinensis from different samples were measured by HPLC. Nine ecological factors including temperature,altitude, moisture and light in different provinces were obtained from the ecological suitability database of the Digital Elevation Model and ArcGIS software packages. The regression equation of the ecological factors and PGGR,polyphyllin Ⅶ,H,I and V contents were established by SPSS 22.0 software using principal component and partial least squares regression method. Result: The total amount of Ⅶ,Ⅵ,Ⅱand I reached the minimum standard of 0.6% in the 2015 edition of Chinese Pharmacopoeia, the general quality of the herbs are higher growing in the Qingchuan,Hongya,Bazhong in Sichuan province. Ecological factors,such as annual sunshine time,maximum air temperature in July,average air temperature in July were a negative correlation with polyphyllin Ⅶ and PGGR,the minimum air temperature in January and average air temperature in January showed a positive correlation with the two saponins ingredients.Annual sunshine time and maximum air temperature in July and average air temperature in July were a positive correlation with polyphyllin H,I and V;elevation,relative humidity,annual precipitation,average air temperature in January showed a negative correlation with the three saponins ingredients. Conclusion: The results provided theoretical support for good quality cultivation of P. polyphylla var.chinensis.关键词：Paris polyphylla var. chinensis;saponins;ecological factors;partial least squares regression;variable importance in projection7|6|6更新时间：2024-01-04
- 摘要：Objective: To establish a method for content determination of 4 kinds of isoflavones in Hedysari Radix and Astragali Radix in Gansu province, and compare the contents of 4 kinds of isoflavones from one year old and two years old samples from different areas of Gansu province. Method: Isoflavone glycosides, formononetin, myrion isoflavones and formononetin were used as the reference substance. Agilent Eclipse-C18 column (4.6 mm×250 mm, 5 μm) was used with acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution. The detection was conducted at the wavelength of 254 nm. Result: The isoflavone glycosides, formononetin, myrion isoflavones and ononin showed good linear relationship with the peak area at the range of 2.25-0.005 625 μg (r=0.999 7), 2.22-0.111 μg (r=0.999 4), 0.112-0.001 12 μg (r=0.999 9), and 1.68-0.001 68 μg (r=0.999 8) respectively. The average recoveries of Hedysari Radix and Astragali Radix were 95%-105% with RSD<3% (n=6). Conclusion: The method for the content determination of 4 kinds of isoflavones in Hedysari Radix and Astragali Radix was simple, accurate and reproducible. It can be used for the content determination of four isoflavones in Hedysari Radix and Astragali Radix from Gansu province and its comparative study. From the content analysis of 4 kinds of isoflavones, it was found that the quality of one year old Hedysari Radix was equivalent to that of two years old Hedysari Radix; one year old Astragali Radix had better quality than two years old Astragali Radix; and the quality of Astragali Radix was better than that of Hedysari Radix. The best producing area for Hedysari Radix was Wudu, and the best producing area for Astragali Radix was Tanchang. Formononetin content in Gansu Hedysari Radix was higher than that in Astragali Radix, while the contents of isoflavone glycosides, myrion isoflavones and ononin were lower than those in Astragali Radix. Therefore, the selection shall be made according to the isoflavones content during development and utilization of Gansu Hedysari Radix and Astragali Radix, and these two herbs are not suitable for alternative use.关键词：Hedysari Radix;Astragali Radix;isoflavones;high-phase liquid chromatography;content determination;Gansu6|9|3更新时间：2024-01-04
- 摘要：Objective: To study the chemical constituents of the ethyl acetate extract from the roots of Elaeagnus pungens. Method: The ethanol extract from the roots of E. pungens were partitioned with petroleum ether, ethyl acetate and n -butanol. The compounds from ethyl acetate extract were separated by silica gel chromatography, sephadex LH-20. Their structures were elucidated by spectral data and physicochemical properties. Result: Thirteen compounds, namely ursolic acid (1), daucosterol (2), quercetin (3), kaempferol (4), β -sitosterol(5), gallic acid (6), ethyl gallate (7), oleanolic acid (8), stigmasterol (9), hydroxyphenylacetic acid (10), hydroxybenzoic acid (11), naringenin (12) and kaempferol 3-O-β-D -glucopyranoside (13) were isolated and identified. Conclusion: Compounds 1-13 were isolated from this plant for the first time.关键词：Elaeagnus pungens;chemical constituent;triterpenoids;flavonoids4|6|3更新时间：2024-01-04
- 摘要：Objective: To establish a systematic quality control method for different parts of Lagerstroemia indica, including qualitative and quantitative analysis by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC), in order to determine alcohol-soluble extractives, ash and loss on drying, respectively. Method: TLC was applied, and the development solvent system was a mixture of acetone, petroleum ether formic acid (3:5:0.2), and the examination was under UV light at a wavelength of 365,254 nm and daylight. HPLC was employed to determinate ellagic acid in different parts of L. indica, in which chromatographic separation was performed on the column Ultimate® AQ-C18 (4.6 mm×250 mm, 5 μm) with the temperature at 40℃. Acetonitrile -0.2% and aqueous phosphoric acid (15:85) were taken as the mobile phase for isocratic elution, the flow rate was 1.0 mL · min-1, and the detection wavelength was 254 nm. Alcohol-soluble extractives, total ash and loss on drying were measured based on Chinese Pharmacopoeia 2015 version. Result: The spots in TLC chromatograms of different parts of L. indicae from different batches and regions were clear, and the Rf value was appropriate. There was a good linear relationship (r=0.999 8) of ellagic acid at the concentration range between 0.276 8-55.36 mg · L-1, and the average recovery rates of flos, folium and cortex of L. indicae were 99.64%, 99.73% and 99.40%, respectively. There were differences in ellagic acid concentration in various batches of samples, in which flos was higher than folium and cortex. The average contents of alcohol-soluble extractives were 18.61%, 18.67% and 3.78%, the average ash contents were 6.50%, 7.47% and 5.62%, and the losses on drying content were 10.51%, 11.41% and 14.22% in flos, folium and cortex of L. indicae, respectively. Conclusion: The method was simple, accurate and reliable, and can provide the basis for the more effective quality control of different parts L. indica.关键词：Lagerstroemia indica;flower;leave;bark;ellagic acid;clearing heat and detoxicating7|6|2更新时间：2024-01-04
- 摘要：Objective: To study the element spectra of six kinds of calcium mineral drugs, in order to establish a rapid and environmental friendly analytical method to identify the authenticity and quality of mineral drugs powder, including the similar composition samples. Method: In this experiment, samples of Gypsum Fibrosum, Stalactitum, Ophicalcitum, Fluoritum, Os Draconis and Spirifer were crushed to 200-mesh powder. Afterwards the samples were pressed into directly pellets, and determined by X-ray fluorescence standard-less quantitative analysis method. All of the samples were measured within the scope of elements from oxygen to uranium. A method for characterizing the spectral characteristics of mineral elements were established, with the square root of K, L, M line intensity as the vertical coordinate and 2θ angle as the horizontal coordinate. The matched element spectral line coordinates were drawn on the spectra, so as to visually identify the elemental characteristics of medicinal materials. Result: From the six kinds of calcium mineral drug, 25 elements, namely Al, Ca, Er, F, Fe, Ga, K, Mg, Mn, Mo, Na, Nb, O, P, Rb, S, Ba, Si, Sr, Zr, Ti, W, Y, Zn and Re, were detected. The element spectra of the same kind calcium mineral drugs were relatively stable, the element spectra of different medicinal materials were obviously different. Among them, the content of Ca, F, O, P, S, Si varied widely, with small changes in Al, Er, Fe, Ga, K, Mo. The content of three Gypsum Fibrosum samples and one Fluoritum sample did not meet the requirement of 2015 edition Chinese Pharmacopoeia. Conclusion: Compared with other methods, X-ray fluorescence spectrometry has the advantages of simple operation, simultaneous analysis of multi-elements and environmental friendliness. It can quickly and easily obtain the elements spectra information of mineral drugs and identify the homogeneity of mineral drug powder.关键词：calcium mineral drug;element spectrum;X-ray fluorescence;standard-less quantitative analysis method6|6|3更新时间：2024-01-04
- 摘要：Objective: To qualitatively identify components of Tanreqing injection in rat blood based on UPLC-Q-TOF/MS. Method: The chromatographic separation was carried out on an Agilent Eclipse Plus C18 column,mobile phase was 0.1% formic acid in water and acetonitrile for gradient elution.Electrospray ionization (ESI) was adopted to obtain data under positive and negative mode,the data was collected in the range of m/z 100-1 100,and components were identified by comparing with reference substances,analysing MS and retrieving literatures. Result: A total of 27 components from Tanreqing injection were identified in rat blood,main metabolites and metabolic pathways of five main components (caffeic acid,chlorogenic acid,baicalin,ursodesoxycholic acid and chenodeoxycholic acid) were obtained.Caffeic acid and chlorogenic acid could product a variety of elimination products of phase Ⅰ and Ⅱ with diverse metabolic pathways;baicalin,ursodesoxycholic acid and chenodeoxycholic acid were mainly elimination of phase Ⅱ. Conclusion: Only some of the secondary components in Tanreqing injection can not be absorbed into the blood.This UPLC-Q-TOF/MS can rapidly identify bioactive compounds of Tanreqing injection and the main metabolic status of them.关键词：Tanreqing injection;components absorbed into blood;metabolites;caffeic acid;baicalin;chlorogenic acid;ursodesoxycholic acid6|7|6更新时间：2024-01-04
- 摘要：Objective: To study the effect of Qingchang Huashi granule on β2 adrenergic receptor(β2AR)/β-arrestin2/nuclear transcription factor-κB(NF-κB) signaling pathway in rat and human ulcerative colitis. Method: Eight rats were randomly selected as control group, while the other rats were used to duplicate the ulcerative colitis model induced by trinitrobenzene sulfonic acid; after successful modeling, the rats were randomly divided into model group, salazosulfapyridine(SASP) group (1.0 g · kg-1), and low, medium and high-dose Qingchang Huashi granule groups (2.8, 5.5, 11.0 g · kg-1), with 8 rats in each group. The drugs were given once a day by gavage for 15 days. The levels of macrophage mobile inhibitory factor(MIF), matrix metalloproteinase-1(MMP-1), macrophage migration inhibitory factor (MIF), matrix metalloproteinase-1 (MMP-1), MMP-2, MMP-9, insulin-like growth factor-1 (IGF-1), superoxide dismutase (SOD),malondialdehyde (MDA), myeloperoxidase (MPO), nitric oxide (NO), nitric oxide synthase (iNOS), glutathione peroxidase (GSH-Px) in experimental UC rats were measured by enzyme linked immunosorbent assay method(ELISA); immunohistochemical method was used to detect the location expressions of β2AR, β-arrestin2, NF-κB in rat models; tumor necrosis factor alpha and lipopolysaccharide were used to induce the HT-29 cell inflammation model; the effect of Qingchang Huashi granule on cell growth was detected by MTT method, the expressions of β2AR, β-arrestin2, NF-κB were measured with Western blot. Result: The injury score of colonic mucosa in model group rats was significantly higher than that of the normal group, expressions of IGF-1, SOD, GSH-Px, β2AR and β-arrestin2 decreased significantly, while expressions of MIF, MMP-2, MMP-9, MDA, MPO, NO, iNOS and NF-κB increased in UC model group (P<0.05,P<0.01). After intervened with Qingchang Huashi granule, these indicators all recovered to normal. Conclusion: Qingchang Huashi granule can reduce the pathological process of ulcerative colitis through β2AR/β-arrestin2/NF-κB signaling pathway.关键词：ulcerative colitis;Qingchang Huashi granule;β2-adrenergic receptor;β-arrestin2;nuclear transcription factor-kappaB10|5|5更新时间：2024-01-04
- 摘要：Objective: To investigate the efficacy of Dongbing Xiazhi Fang (DXF) on asthmatic guinea pigs through acupoint-application, and its effect on intestinal flora of asthmatic guinea pigs. Method: Seventy five guinea pigs were randomly divided into blank control group, asthmatic model group, positive control group, acupoint-application group and non-acupoint-application group (n=15). The asthmatic model of guinea pigs was established through ovalbumin injection and aerosol inhalation, except for the blank control group. The hematoxylin-eosin(HE) and Masson staining pathologic sections of lung tissue in asthma were observed. The total genomic DNA was extracted from guinea pig feces, and the 16 S rRNA V4 variable region gene was amplified by PCR and sequenced by Illumina HiSeq. The gene sequences with the similarity of more than 97% were clustered, and analyzed by bioinformatics. Result: Compared with the asthmatic model group, the other groups of guinea pigs showed reduced inflammatory cells, bronchoconstriction, and alleviated airway epithelial hyperplasia and good recovery and various degrees of reduced collagen fiber deposition. The positive group and the acupoint-application group showed a more obvious degree of reduction, and the therapeutic effect of the acupoint-application group was better than that of the non-acupoint-application group. The analysis of operational taxonomic unit (OTU) and its abundance indicated that the proportion of various bacteria changed after the onset of asthma. The diversity of intestinal flora of guinea pigs significantly increased after being treated with DXF and Prevotella. The proportion of Bacteroides in the positive control group and other administration groups were less than that in the asthmatic model group, while the proportion of Proteobacteria and Firmicutes were more than that in the asthmatic model group. Conclusion: DXF has an effect in treating asthma through transdermal acupoint-application, and can alleviate asthma symptoms, regulate the composition of intestinal flora in asthmatic guinea pigs. The results suggest that the prescription have the efficacy on asthma by affecting the body immunity and regulating the intestinal flora, and provide a theoretical basis for studying the treatment mechanism of asthma.关键词：Dongbing Xiazhi Fang;acupoint application;asthma;intestinal flora;16 S rRNA V4 variable region;Illumina HiSeq sequencing4|6|5更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of Xinji Erkang (XJEK) on human umbilical vein endothelial cells (HUVECs) injury induced by angiotensin Ⅱ (AngⅡ). Method: HUVECs were cultured in vitro and randomly divided into 7 groups as follows:control group, AngⅡ (1×10-5 mol · L-1) group, and AngⅡ (1×10-5 mol · L-1)+XJEK groups (0.1, 0.2, 0.4, 0.8, 1.6 g · L-1). Thiazole blue (MTT) assay was used to examine HUVEC viability. The level of reactive oxygen species (ROS) and the intracellular free calcium concentration were measured by flow cytometry and Calcium Imager respectively. The content of nitric oxide (NO), malondialdehyde (MDA) and superoxide dismutase (SOD) were detected by colorimetric and TBA analysis. Western blot was applied to determine the expression of endothelial nitric oxide synthase (eNOS) protein. Result: Compared with the control group, the Ang Ⅱ group showed significant reduction in endothelial cell vitality, NO release and eNOS protein expression, and significant increase in MDA and ROS content and Ca2+ concentration in endothelial cell cytoplasm (P<0.05, P<0.01). Compared with the AngⅡ group, XJEK could obviously improve endothelial dysfunction (ED) by promoting eNOS activities and enhancing NO in a dose-dependent manner. Moreover, XJEK could up-regulate SOD activity and down-regulate MDA content significantly. In addition, after treatment with XJEK, ROS level and intracellular Ca2+ concentration in endothelial cells were decreased compared with the AngⅡgroup (P<0.05). Conclusion: These results suggest that XJEK has a protective effect on AngⅡ-induced HUVECs injury, and the mechanism underlying may contribute to inhibiting intracellular Ca2+ overload and improving ED and ameliorating ROS.关键词：Xinji Erkang;Astragali Radix;Ginseng Radix et Rhizoma;Polygonati Odorati Rhizoma;human umbilical vein endothelial cells (HUVECs);angiotensin Ⅱ;calcium ion6|6|1更新时间：2024-01-04
- 摘要：Objective: To investigate the protective effect and mechanism of combined formula of Erigerontis Herba and Paeoniae Radix Rubra (Xinshao) on cerebral ischemia reperfusion injury in rats. Method: The rats were randomly divided into four groups:sham operation group, model group, nimodipine group (12.6 mg · kg-1) and Xinshao group (12.5 g · kg-1). Rats were administrated with drugs for 3 days; then the cerebral ischemia-reperfusion model was established by improved Zea longa method. After surgery, the rats were administrated with drugs for 7 days. The neurological behavior was evaluated by Zea Longa method.triphenyltetrazolium Chloride(TTC) staining was performed to determine the area of cerebral infarction. interleukin-6(IL-6), IL-1β and tumor necrosis factor-α(TNF-α) levels in serum were examined by enzyme-linked immunosurbent assay(ELISA). The levels of phosphorylation of p65 and IκBα were detected by Western blot. Result: Compared with sham operation group, the score of neurological behavior and the percentage of infarct area were significantly increased in model group, which indicated the successful establishment of the MACO model. Xinshao could significantly ameliorate the neurological behavior (P<0.05), and decrease the percentage of infarct area (P<0.05). Compared with model group, the levels of IL-6, IL-1β and TNF-α in serum were significantly lowered in Xinshao group (P<0.01). Western blot analysis showed that Xinshao could reduce the phosphorylation levels of p65 and IκBα (P<0.01). Conclusion: Xinshao has a protective effect on cerebral ischemia reperfusion injury in rats, which may be associated with the inhibition of nuclear transcription factor-κB(NF-κB) pathway.关键词：Erigerantis Herba;Paeoniae Radix Rubra;cerebral ischemia reperfusion;nuclear transcription factor-κB (NF-κB) pathway5|5|11更新时间：2024-01-04
- 摘要：Objective: To compare the effect of aspirin on antiplatelet aggregation before and after the combined administration with Notoginseng total saponins, in order to investigate the effect of Notoginseng total saponins on the efficacy of aspirin. Method: A total of 24 rats were randomly divided into 4 groups:control group, Notoginseng total saponins group (NTS, 31.25 mg · kg-1), aspirin group (ASA, 20.83 mg · kg-1), and combination group (NTS, 31.25 mg · kg-1 +ASA, 20.83 mg · kg-1). All of the experimental groups were intragastrically administered with the corresponding concentrations of liquid, except for control group. Blood samples were taken at 5, 30, 60, 120, 240, 480 min, the levels of thromboxane A2 (TXA2), cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) in serum were determined by enzyme-linked immunosurbent assay(ELISA). Result: The contents of COX-1 and COX-2 in ASA group and blank control group showed no significant difference. In group NTS, the content of COX-1 between 5-240 min was significantly lower than that in the blank group (P<0.01), and the content of COX-2 was significantly lower than that in the blank group (P<0.01) at 30, 60,120,480 min after drug administration. The COX-1 content of ASA and NTS combination group between 5-60 min was significantly lower than that in blank group and ASA group (P<0.05), and the COX-2 content between 5-240 min was significantly lower than that in blank group and ASA group (P<0.01). The content of TXA2 in serum of each experimental group was significantly lower than that in blank control group (P<0.05) between 5-240 min, in which that of combination group were significantly lower than that in aspirin group (P<0.05) at 30 min, 60 min and 240 min after drug administration. The inhibitory effect of the combination group on TXA2 reached a peak at 60 min after administration. Conclusion: The combined administration of NTS and aspirin showed a better effect in antiplatelet aggregation than the single administration of aspirin or NTS. Their inhibitory effect on TXA2 is an additive action, in which NTS can inhibit the expressions of COX-1 and COX-2, which may be one of the ways to enhance aspirin's anti-platelet aggregation.关键词：aspirin;Notoginseng total saponins;anti-platelet agglutination;thromboxane A2(TXA2);cyclooxygenase-1(COX-1);COX-27|6|4更新时间：2024-01-04
- 摘要：Objective: To study the effect of Qiangxinkang granule on the pathomorphology of cardiac myocytes and the expressions of adenylate translocation enzyme(ANT) and peroxisome proliferator activated receptor γ coactivator-1 alpha (PGC-1α) mRNA in rats with heart failure. Method: Healthy Wistar rats were selected and injected with adriamycin through abdominal cavity (3 mg · kg-1, once a week) for six weeks to make the model of HF rats. Then they were divided into seven groups:blank group, model group, Qili Qiangxin group (0.65 g · kg-1), Trimetazidine hydrochloride group (10.8 mg · kg-1), and high, medium and low-dose Qiangxinkang granule groups (20, 10, 5 g · kg-1). After modeling, the cardiac function was tested to verify the successful modeling. Four weeks later, htoxylin eosin (HE) staining and electron microscopy were used to observe the pathological changes of myocardial cells, and quantitative polymerase chain reaction (Real-time PCR) was used to observe the pathological changes of myocardial cells and the expressions of adenylate translocation enzyme (ANT) mRNA and PGC-1α mRNA in the seven groups. Result: Compared with the blank group, the model group showed significant slowdown in the heart rate, reduction in the maximum rising and falling rate at the left ventricle, obvious decrease in the left ventricular systolic pressure, remarkable increase in the left ventricular end-diastolic pressure and significant decrease in systolic pressure, diastolic pressure and average blood pressure (P<0.01), indicating the establishment of the heart failure model; the model group showed more significant myocardial cell lesions and more obvious alleviation in myocardial cell lesions, and decrease in ANT, PGC-1α mRNA content (P<0.01). Compared with the model group, Trimetazidine hydrochloride group, qili cardiac tonic group, and high and medium-dose Qiangxinkang granule groups showed a significant increase in ANT, PGC-1α mRNA content (P<0.01); the low-dose Qiangxinkang granule group showed a notable increase in ANT, PGC-1α mRNA content, with a statistical significant difference compared with the model group (P<0.05). Conclusion: Qiangxinkang granule could improve myocardial cell structure, significantly increase adenylate transposition enzyme (ANT) mRNA and PGC-1 mRNA content. Qiangxinkang granule can strengthen the cardiac function by improving myocardial cell energy metabolism.关键词：chronic heart failure;Qiangxinkang granule;adenylate translocation enzyme (ANT);peroxisome proliferator activated receptor γ coactivator-1 alpha (PGC-1α)8|5|3更新时间：2024-01-04
- 摘要：Objective: To study the protective effect of Yishen Yangyin mixture on lupus nephritis with systemic lupus erythematosus (SLE) and its molecular mechanism. Method: MRL/lpr model mice were given Yishen Yangyin mixture (17.25 g · kg-1) by gavage after the onset of disease, while the positive control group was given prednisone by gavage (0.65 mg · kg-1). Normal C57 background mice and MRL/lpr mice (experimental control group) were administered with normal saline, 2 times a day, for consecutively 28 days; urine was collected every 7 days to detect the urine protein changes. After twenty-eight day, immunofluorescence staining was used for detecting kidney deposition IgG. Raybiotech antibody arrays was used to detect the changes of 308 cytokines; enzyme linked immunosorbent assay (ELISA) was used to confirm some changed factors (mouse kidney tissue and serum level of patient); Western blot was used to detect Janus kinase2/signal transduction and transcrptional activators(JAK2/STAT3) signal, stromal cell derined facto-1/chemokine receptor4(SDF1/CXCR4) signal axis, expression of T helper 17 cells(TH17) cells secreted interleukin-17(IL-17). Result: In the Yishen Yangyin mixture group and the prodnisone group, with the extension of medication time, urine protein content decreased gradually, particularly in the fourth week (P<0.05); 28 days later after administration, compared with the MRL/lpr group (experimental control group), the Yishen Yangyin mixture group and the prodnisone group showed decrease in kidney IgG deposition (P<0.05). According to Raybiotech antibody array screening, compared with normal C57 mice, the expression of kidney SDF1/CXCR4 signal in MRL/lpr mice was increased (P<0.01). Compared with MRL/lpr mice, the expression of SDF1/CXCR4 signal in Yishen Yangyin mixture group was significantly decreased (P<0.01). The results of SDF1/CXCR4 ELISA were consistent with the results of antibody array. Western blot assay showed that compared with normal C57 mice, the JAK2/STAT3 phosphorylation protein expression in kidney tissue of MRL/lpr mice was increased (P<0.05); meanwhile, the SDF1,CXCR4, IL-17 protein expression were significantly increased (P<0.05).When MRL/lpr mice was treated with Yishen Yangyin mixture and hormone by gavage for 28 days, compared with the MRL/lpr group, the phosphorylation level of JAK2,STAT3, the expression of SDF1,CXCR4 and the secretion of IL-17 significantly decreased (P<0.05). Conclusion: Yishen Yangyin mixture can reduce the urinary protein level of MRL/lpr mice, and can play a key role in renal protection by inhibiting activation of JAK2/STAT3 and SDF1/CXCR4 signaling pathway and decreasing the activity of T helper 17 cells and the secretion of IL-17.关键词：Yishen Yangyin mixture;lupus erythematosus;Janus kinase2/signal transduction and transcrptional activators (JAK2/STAT3);stromal cell derined facto-1/chemokine receptor4(SDF1/CXCR4);interleukin-17(IL-17)4|6|2更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of Bistortae Rhizoma n-butyl alcohol extract (PBNA-413) on cardiocyte apoptosis in rats with myocardial ischemia-reperfusion(MI/R) injury. Method: Thirty male Sprague-Dawley rats were randomly divided into five groups:sham group, MI/R model groups, low-dose, middle-dose and high-dose (0.1, 0.3, 1.0 mg ·kg-1) PBNA-413 treatment groups (n=6). The models of MI/R injury were established by ligating the left anterior descending coronary artery for 40 min and then reperfusing for 60 min, and the electrocardiogram (ECG) was recorded. Western blot analysis and Real-time PCR were utilized to measure B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), cysteine aspartate protease-3 (Caspase-3) and Caspase-9 mRNA and protein expressions in the ischemic and reperfusion region. Result: After myocardial ischemia, ECG ST-segment and T-wave in MI/R vehicle group were significantly higher than those in sham group(P<0.05,P<0.01), but they were significantly decreased in the PBNA-413 treatment groups (P<0.01), particularly in middle and high-dose groups. Compared with Sham group, protein and mRNA expressions of Bcl-2 (P<0.01) were decreased, while Bax, Caspase-3 and Caspase-9 were increased in MI/R vehicle group(P<0.05,P<0.01). Compared with MI/R vehicle group, the middle-dose PBNA-413 group showed an increased protein expression of Bcl-2 (P<0.05,P<0.01), but decreased expressions of Bax, Caspase-3 and Caspase-9 (P<0.05,P<0.01). Except for Caspase-9, mRNA expressions of Bax, Bcl-2, and Caspase-3 showed similar changes with protein expressions. Conclusion: PBNA-413 may play a protective role against MI/R injury in rats by inhibiting cell apoptosis.关键词：Bistortae Rhizoma n-butyl alcohol extract (PBNA-413);myocardial ischemia/reperfusion;apoptosis;B-cell lymphoma-2(Bcl-2);Bcl-2 associated X protein (Bax);cysteine aspartate protease (Caspase)-3;caspase-95|6|1更新时间：2024-01-04
- 摘要：Objective: To investigate the effects of baicalin on ventricular remodeling, ventricular myocyte apoptosis and β1-adrenoceptor(β1-AR)/protein kinase A (PKA)/Ca2+/calmodulin-dependent priotein kinase Ⅱ (CaMK Ⅱ) signaling pathway in rats with dilated cardiomyopathy induced by adriamycin(ADR). Method: Sixty male Wistar rats were randomly divided into normal group, model group, baicalin low, middle and high dose groups and Carvedilol group. The model group received intraperitoneal injection of ADR 2 mg · kg-1; Baicalin and Carvedilol groups were given with baicalin (25, 50, 100 mg · kg-1), carvedilol 10 mg · kg-1 · d-1 gavage on the basis of model group; while the normal group was given with an equal volume of 0.9% NaCl by intraperitoneal injection, 1 time/week, for a total of 3 times.At the end of 7 weeks, the ventricular ultrasonography and heart function were measured by ultrasonography. The levels of N-terminal pro-brain natriuretic peptide (NT-proBNP) and human stromelysin-2(ST2)in serum were detected by enzyme-linked immunosorbent assay (ELISA). The apoptosis of ventricular myocytes was observed by TdT-mediated dUTP nick-end labeling(TUNEL) staining. The expression levels of β1-AR, PKAand CaMK Ⅱ in ventricular myocytes were detected by Western blot. Result: As compared with the normal group, the rats in the model group showed obvious ventricular remodeling and decreased cardiac function(P<0.05); the levels of NT-proBNP and ST2 were increased (P<0.05); the number of apoptotic cells was increased (P<0.05), and the expression levels of β1-AR, PKA and CaMKⅡwere also increased. As compared with the model group, the ventricular remodeling and cardiac function were improved in baicalin and carvedilol groups; the levels of NT-proBNP and ST2 were decreased (P<0.05), and the number of apoptotic cells was decreased (P<0.05); and the expression levels of β1-AR, PKA and CaMK Ⅱ in ventricular myocardium were also decreased (P<0.05). Conclusion: Baicalin can effectively improve the ventricular remodeling and decrease the apoptosis of cardiomyocytes in ADR-induced dilated cardiomyopathy rats. The mechanism may be related to the inhibition of β1-AR/PKA/CaMK Ⅱ signaling pathway in ventricular myocytes.关键词：baicalin;dilated cardiomyopathy;ventricular remodeling;apoptosis;β1-adrenoceptor (β1-AR)/protein kinase A (PKA)/Ca2+/calmodulin-dependent priotein kinase Ⅱ(CaMK Ⅱ) signaling pathway7|5|7更新时间：2024-01-04
- 摘要：Objective: To study the effects of baicalein on ultraviolet B (UVB)-induced apotosis and the expression of related cytokines of human keratinocytes (HaCaT cells). Method: The HaCaT cells were cultured in vitro, and the logarithm of the cells was taken. Cells were randomly divided into blank control group,model group and baicalein group. The irradiation of UVB was given at irradiation dose of 30 mJ · cm-2 to establish photoaging model for model group and baicalein group. The method of methyl thiazolyl tetrazolium(MTT) was used to screen the effective and safe concentration of baicalein and screen the effects of baicalein on the proliferation rate of photoaging HaCaT cells; the content of reactive oxygen species(ROS) in each group was determined by reactive oxygen free radical (ROS) kit;the apoptosis of HaCaT cells was determined by the Annexin V-FITC apoptosis detection kit; and Western blot method was used for detecting the effects of baicalein on B-cell lymphoma-2 (Bcl-2),cysteinyl aspartate-3(Caspase-3) and Caspase-9 protein expression. Result: The best safe and effective concentrations of baicalein were 1×10-7, 1×10-6, 1×10-5 mol · L-1.As compared with the blank group,the content of ROS and the apoptosis rate of the model group were significantly increased(P<0.01);the expression of Bcl-2 protein was significantly decreased (P<0.01); and the expression levels of Caspase-3 and Caspase-9 protein were significantly increased (P<0.01).As compared with the model group, the content of ROS and the apoptosis rate in 1×10-7,1×10-6, 1×10-5 mol · L-1 baicalein groups were significantly decreased (P<0.05,P<0.01); the expression of Bcl-2 protein was significantly increased (P<0.05, P<0.01); and the expression levels of Caspase-3 and Caspase-9 protein were significantly decreased (P<0.05,P<0.01). Conclusion: Baicalin can reduce the content of ROS and the apoptosis rate of HaCaT cells, regulate the expression of Bcl-2 protein in Bcl-2 family,decrease the expression of related cytokines Caspase-3 and Caspase-9, and inhibit the apoptosis induced by UVB.关键词：baicalin;ultraviolet B;human keratinocytes;apoptosis7|6|1更新时间：2024-01-04
- 摘要：Objective: To study the effect of Nepeta angustifolia extract on hematoma in intracerebral hemorrhage model rats and its possible mechanism. Method: The intracerebral hemorrhage model was established by injecting with collagenase. Male SD rats were divided randomly into normal control group, sham operation group, model group, large-dose, medium-dose and small-dose (6.4, 3.2, 1.6 g · kg-1) N. angustifolia groups, nimodipine group. Neurological behavior was observed after treatment for 7 days, the neurobehavioral score of each group was evaluated by Longa score method. Subsequently, the rats were put to death, and the hemorrhagic hemisphere was measured to determine the wet weight. After drying, the dry weight of the rat brain was measured, and the brain water content of the rat brain was calculated by the Tada formula. The brain tissues of the rats was collected and made into slices. The volume of cerebral hematoma of the rats was observed by sections. Immuno fluorescence was used to observe the expressions of aquaporin-4 (AQP-4) and nerve growth factor (NGF). Result: The neurological behavior score of N. angustifolia groups was lower than that of the model group (P<0.05). The hematoma was not significantly removed, the brain edema of large-dose N. angustifolia group was significantly reduced (P<0.05). Compared with the model group, the expression of AQP-4 in rats treated with N. angustifolia decreased significantly (P<0.05), the expression of NGF of the medium-dose group increased significantly (P<0.05,P<0.01). Conclusion: N. angustifolia extract has a protective effect on neurobehavioral score and content of brain water, and can inhibit expressions of AQP-4 and NGF, so as to treat intracerebral hemorrhage.关键词：Nepeta angustifolia extract;intracerebral hemorrhage;aquaporin-4(AQP-4);nerve growth factor (NGF)5|7|2更新时间：2024-01-04
- 摘要：Objective: To explore the effect and mechanism of Jinzhen oral liquid (JZOL) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in mice. Method: Mice were randomly divided into normal control group, model group, dexamethasone group (DEX), JZOL high (JZOLG), medium (JZOLZ) and low (JZOLD) dose (4.4, 2.2, 1.1 g · kg-1) groups. Corresponding dose of medicine was given in the treatment groups, and the mice in normal group and model group received the same dose of normal saline, once a day for 7 days. Then the ALI models were induced by intraperitoneal injection of LPS (10 mg · kg-1), while the mice in normal group received intraperitoneal injection of the same dose of normal saline. Six hour later, the lung tissues were taken; left lung wet-to-dry ratio (W/D) was measured; tumor necrosis factor (TNF)-α, and interleukin(IL)-1β in lung tissues were detected by enzyme-linked immunosorbent assay (ELISA); the protein expression levels of p65, IκBα, ERK1/2, p38 protein and their phosphorylation levels in lung tissues were detected by Western blot. Result: As compared with the normal group, the W/D, levels of TNF-α, IL-1β and the phosphorylation expression levels of p65, IκB, p38 protein were increased in the model group(P<0.01). As compared with the model group, the W/D was significantly decreased in JZOLD, JZOLG and DEX groups; the levels of TNF-α and IL-1β were decreased significantly, and the phosphorylation expression levels of p65, IκB, p38 protein were significantly down-regulated in JZOLG, JZOLZ, JZOLD, and DEX groups(P<0.05,P<0.01); and the phosphorylation expression level of ERK1/2 was significantly down-regulated in JZOLG and DEX groups(P<0.05,P<0.01). Conclusion: JZOLY can effectively improve the interstitial edema of the lung tissues and reduce inflammatory cytokines in mice with LPS-induced ALI. The mechanism is closely associated with inhibiting the phosphorylation levels of p65, IκBα, ERK1/2 and p38, blocking nuclear transcription factor-kappa B (NF-κB) and mitogen activated protein kinase (MAPK) inflammatory pathway.关键词：Jinzhen oral liquid;acute lung injury;lipopolysaccharide (LPS);mitogen activated protein kinase (MAPK);nuclear transcription factor-kappa B (NF-κB)8|5|11更新时间：2024-01-04
- 摘要：Objective: To explore the mechanism of Jinfukang oral liquid (JFK) in inducing the apoptosis of non-small cell lung cancer cell line A549. Method: The non-small cell lung cancer cell lines A549 in logarithmic phase of growth were randomly divided into the control group and drug treatment groups. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay was used to investigate the effects of different concentrations of JFK (0.3, 1.5, 3, 7.5, 15, 30 g · L-1) on the proliferation of A549 cells in vitro. The plate colony formation assay and flow cytometry were used to observe the effects of JFK on the colony formation and apoptosis of A549 cells, respectively. Protein expressions of cleaved PARP, active Caspase-3, β-catenin, cyclinD1, and the mitogen-activated protein kinase(MAPK)/proteinkinse B(Akt) signaling pathways were investigated by Western blot. Cells were divided into control group and drug groups treated at the concentrations of 15, 30 g · L-1, and each group was intervened for 24 h or 30 min. Result: JFK inhibited the proliferation of A549 cells in a dose-dependent manner within the range from 1.5-30 g · L-1(P<0.01). JFK inhibited colony formation at 15-30 g · L-1(P<0.01). JFK increased the ratio of Annexin V and PI double positive cells at the concentrations of 15-30 g · L-1, suggesting that JFK induced apoptosis in A549 cells (P<0.05). The protein expressions of cleaved PARP and activated Caspase-3 were increased, while the expressions of β-catenin and cyclinD1 were suppressed in a dose-dependent manner (P<0.05, P<0.01) after treatment with JFK. And the phosphorylation levels of JNK and p38 were up-regulated, while that of T308 and S473 sites of Akt were down-regulated (P<0.05). Conclusion: Jinfukang decoction inhibited the proliferation and colony formation of non-small cell lung cancer cells A549.JFK induced apoptosis with the increase of the protein expressions of cleaved PARP and activated Caspase-3, and the decrease of the protein expressions of β-catenin and cyclinD1, partly through JNK/p38/MAPK and Akt signaling pathways.关键词：Jinfukang oral liquid;non-small cell lung cancer;apoptosis;mitogen-activated protein kinases signaling pathway;Akt signaling pathway5|6|4更新时间：2024-01-04
- 摘要：Objective: To investigate the clinical efficacy of Dashen injection combined with ganglioside in the treatment of American Spinal Injury Association(ASIA) grade C and D spinal cord injury (SCI) patients, in order to explore its mechanism. Method: A total of 98 patients with spinal cord injury were randomly divided into Dashen injection group (30 cases), ganglioside group (31 cases) and combined group (37 cases). In addition to rehabilitation training, Ganglioside group were also given intravenous ganglioside treatment, 60 mg, 1 time/d; in addition to rehabilitation training, Danshen injection group was also given Danshen injection through intravenous infusion, 10 mL, 1 time/d; combined group was treated with Danshen, in addition to ganglioside therapy. The treatment lasted for 20 days and was discontinued for 10 days. The total effective rate and the incidence of adverse reactions were compared between the two groups. At the same time, the levels of inflammatory factors and exercise, pain, sense of tough and other neurological rehabilitation index scores were compared; The levels of tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), interleukin-1β (IL-1β), superoxide dismutase (SOD), malondialdehyde (MDA), Vitamin E (VE), brain-derived neurotrophic factor (BDNF) and astrocyte primary calcium binding protein S-100β (S-100β) in serum were measured by enzyme linked immunosorbent assay (ELISA). Result: The total effective rate was 83.78% in combined group, which was significantly higher than 78.57% of the Danshen injection group and 80.65% of ganglioside group. The ASIA scores of exercise, pain and touch were significantly higher in the combined group than in Danshen injection group and ganglioside group (P<0.05), and the recovery time, the hospitalization time and the talking time in the combined group were significantly shorter than those in other groups (P<0.05). The levels of TNF-α, IL-8 and IL-1β were lower in all of the groups after treatment than those before treatment, but with the lowest levels in the combined group (P<0.05). After treatment, patients of Danshen injection group and combined group showed increase in expressions of serum SOD, VE and decrease in MDA (P<0.05). Conclusion: Dashen injection combined with gangliosides can help ASIA graded C and D grade SCI patients to restore, and prevent secondary spinal cord damage. Its mechanism may be related to anti-inflammation and anti-oxidation.关键词：Dashen injection;ganglioside;spinal cord injury;inflammation;oxidative stress;secondary injury7|5|1更新时间：2024-01-04
- 摘要：Objective: To investigate the effect of modified Bazhentang combined with moxa-moxibustion on the tolerance, pain relief and life quality of patients receiving radiotherapy and chemotherapy for advanced cervical cancer. Method: A total of 106 cases of patients with advanced cervical cancer admitted in our hospital from February 2014 to April 2017 were selected and divided into observation group and control group by random number table, with 53 cases in each group. All of the patients were given radiotherapy and chemotherapy. In addition to radiotherapy and chemotherapy, the observation group was also treated with modified Bazhentang combined with moxa-moxibustion. An evaluation on the short-term efficacy of two groups was carried out after one course of treatment. The cellular immune indexes, nutritional indicator, pain visual analogue scale (VAS), Karnofsky scores (KPS) and incidence of adverse reactions were recorded and compared between the two groups. Result: The overall effective rate of observation group was 86.79%, which was higher than 60.38% of control group (P<0.05). After treatment, the CD3+, CD4+, CD4+/CD8+, NK cells of observation group remarkably increased compared with those before treatment (P<0.05), but those of control group showed no big difference. What's more, the above index levels of observation group were higher than those of control group at the same time points after treatment (P<0.05). Compared with those before treatment, serum albumin, prealbumin and transferrin levels of observation group after treatment gained an evident increase (P<0.05), but those of control group showed a big decrease (P<0.05). After treatment, all serum nutritional indicators were better than those of control group (P<0.05). The pain VAS scores of both groups began to rise after 1 week of treatment, but the scores of observation group after 1, 2, 4 weeks of treatment were much lower than those of control group in the same period (P<0.05). The KBS effective rate of observation group was 84.91%, which was significantly higher than 50.94% of control group (P<0.05). The incidences of aleucocytosis and emesis of observation group respectively were 35.85% and 18.87%, which were much lower than 56.60% and 52.83% of control group (P<0.05). Conclusion: In treatment of advanced cervical cancer, modified Bazhentang combined with moxa-moxibustion can significantly improve the effects of radiotherapy and chemotherapy, enhance the immune function, relieve pain, and improve the patients' chemotherapeutic tolerance of patients and their life quality.关键词：modified Bazhentang;moxa-moxibustion;advanced cervical cancer;tolerance;life quality5|6|1更新时间：2024-01-04
- 摘要：Objective: To study the clinical efficacy of modified Gegentang combined with spinal manipulation and its effect on inflammatory factors and pain mediators of cervical disc herniation (CDH). Method: Totally 157 cases of CDH patients treated in our hospital from March 2014 to December 2016 were selected and divided into two groups according to the admitted order, with 78 cases in clinical group and 79 in control group. Patients in control group were treated with spinal manipulation; in addition, the clinical group was also given modified Gegentang. Then the clinical efficacy, visual analogue scale/score (VAS) scores, inflammatory factors and pain mediators were compared. Result: Compared with 82.28% in the control group, the total effective rate of the clinical group was 94.87%, with significant differences (P<0.05). After the treatment for 6 d and 10 d, VAS scores of the two groups decreased, the experimental group was inferior to the control group (P<0.05). Compared with before treatment, tumor necrosis factor (TNF)-α, interleukin (IL)-1 and IL-6 of the two groups decreased after treatment (P<0.05). Compared with control group, the levels of TNF-α, IL-1 and IL-6 in patients of clinical group were lower after treatment (P<0.05). Compared with before treatment, 5-hydroxytryptamine (5-HT), prostaglandin (PEG2) and nitric oxide (NO) of the two groups decreased after treatment (P<0.05). Compared with control group, the levels of 5-HT, PEG2 and NO in patients of the clinical group were lower after treatment (P<0.05). Conclusion: Modified Gegentang combined with spinal manipulation can significantly enhance the therapeutic efficiency, lower VAS scores and the perception of pain, effectively inhibit inflammatory reaction and slow down the progress of cervical intervertebral disc degeneration. Therefore, it has a better effect on CDH and is worth clinical promotion and application.关键词：modified Gegentang;spinal manipulation;cervical disc herniation;clinical efficacy;inflammatory factor;pain mediator6|6|1更新时间：2024-01-04
- 摘要：Objective: To explore the therapeutic effect and safety of modified Yibitang combined with diclofenac sodium in treating lumbar disc herniation (LDH) with kidney deficiency syndrome. Method: A total of 228 cases of LDH with kidney deficiency syndrome were randomly divided into the traditional Chinese medicine(TCM) group, the chemical medicine group and the Chinese and western medicine group, with 76 cases in each group. The TCM group was treated with modified Yibitang; the diclofenac sodium was applied in chemical medicine group; Yibitang and diclofenac sodium were adopted in the Chinese and western medicine group. One course of treatment was 8 weeks. The Oswestry disability index (ODI), the scale for differentiation of syndromes of lumbar disc herniation (TCM symptom), and the integration of the Japanese orthopaedic association waist pain score (M-JOA) were observed. The serum tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), interleukin-6 (IL-6) and C-reactive protein (CRP) were detected before and after treatment. The rates of effective, recurrence and adverse reactions were compared. Result: The efficiency of the Chinese and western medicine group was 97.2%, which was better than 82.4% of the TCM group and 80.3% of the chemical medicine group (P<0.05). ODI, TCM symptom, M-JOA, serum TNF-α, IL-1, IL-6 and CRP were significantly higher than those in Chinese medicine group and western medicine group (P<0.05). In terms of the recurrence rate, the order of the groups was Chinese and western medicine group (1.7%) < the TCM group (9.3%) < the chemical medicine group (22.6%, P<0.05). In adverse reaction rates, the order of the groups was the TCM group (2.7%) < the Chinese and western medicine group (9.5%) < the chemical medicine group (24.6%, P<0.05). Conclusion: Modified Yibitang has no obvious difference from chemical medicine diclofenac sodium in terms of the effect in the treatment of LDH case with Kidney deficiency syndrome, with a lower incidence of adverse events and recurrence rate. Modified Yibitang combined with diclofenac sodium have a synergistic interaction in treating lumbar disc herniation (LDH) with kidney deficiency syndrome, with a better efficacy than single administration of Yibitang and diclofenac sodium.关键词：modified Yibitang;lumbar disc herniation;kidney deficiency syndrome;diclofenac sodium6|6|3更新时间：2024-01-04
- 摘要：Objective: To observe the clinical efficacy of Wenyang Huoxue Jiedu decoction on testosterone and heart rate variability (HRV) in male patients with chronic heart failure (CHD), in order to explore the mechanism of Wenyang Huoxue Jiedu decoction on testosterone and HRV in male patients of chronic heart failure with Yang deficiency. Method: A total of 110 outpatients or inpatients with chronic heart failure by the random number method were divided into treatment group and control group. The control group received standardized western medicine combined with Shenfu decoction, while the treatment group was also given Wenyang Huoxue Jiedu decoction in addition to basic therapy of western medicine. The course of treatment was 28 days. At the end of the course of treatment, the clinical efficacy of the two groups was observed, and the 6-minute walking distance, the Minnesota quality of life score, the testosterone, the left ventricular ejection fraction (LVEF), the HRV and the adverse reactions were recorded. Result: The total effective rate of treatment group (90.91%) was better than that of the control group (84.45%, P<0.05). After treatment, the walking distance, testosterone, LVEF and HRV were significantly higher than those before treatment, whereas the Minnesota quality of life score, NT-proBNP were significantly decreased (P<0.05). Conclusion: The levels of testosterone and HRV in patients with chronic heart failure (CHD) were significantly lower than those in the control group. Wenyang Huoxue Jiedu decoction relieved the degree of Yang deficiency, improved the heart function and the quality of life of male patients with chronic heart failure. Its mechanism may be related to the inhibition of sympathetic activity and the improvement of serum testosterone level and HRV. Traditional Chinese medicine(TCM) has a hormone-like effect and the advantages of overall treatment and multi-target intervention, which reflects the features and significance of TCM in regulating endocrine, and is worth further study.关键词：chronic heart failure;Wenyang Huoxue Jiedu decoction;testosterone;heart rate variability4|6|2更新时间：2024-01-04
- 摘要：Objective: To observe the effect of modified Qiju Dihuang Tang based on syndrome differentiation in treating patients with hypertensive disorder complicating pregnancy (syndrome of hyperactivity of liver due to Yin deficiency) at preeclampsia and cytotoxic factor-vascular endothelial function. Method: One hundred and sixty patients were randomly divided into control group(80 cases) and observation group(80 cases) by random number table. Patients in control group were injected with 25% magnesium sulfate (10 mL) and 5% glucose (100 mL) for successively 7 days, and orally given labetalol hydrochloride tablets, 100 mg/time, 3 times/days. In addition to the therapy of control group, patients in observation group got modified Qiju Dihuang Tang based on syndrome differentiation, 1 dose/day. The treatment continued until the day before termination of pregnancy. And the outcome of pregnancy was recorded, and the cumulative incidence of spontaneous abortion, hypertensive heart disease complicating pregnancy, placental abruption, oligohydramnios, postpartum hemorrhage and dead were calculated. And the cumulative incidence of stillbirth, neonatal respiratory distress syndrome, low body weight after birth, asphyxia of newborn and neonatal death were also recorded. Before and after treatment, the blood pressure level and syndrome of hyperactivity of liver due to Yin deficiency were evaluated, and levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), serum cystatin C(CysC), neuropeptide Y (NPY), serum endothelin-1 (ET-1), nitric oxide (NO), homocysteine (Hcy), von Willebrand factor (vWF) were detected. Result: The cumulative incidence rate of adverse pregnancy outcomes in pregnant women in observation group was 13.16%, which was lower than 76.92% in control group (χ2=6.940, P<0.01). The premature birth rate in observation group was 15.79%, which was lower than 25.64% in control group. The term delivery rate in observation group was 78.95%,which was higher than 62.82% in control group (χ2=4.626, P<0.01). The cumulative incidence rate of fetal adverse outcomes in control group was 32.05%, which was higher than 17.11% in observation group (χ2=4.626, P<0.05). After treatment, levels of SBP and DBP in observation group were lower than those in control group, and scores of syndrome of hyperactivity of liver due to Yin deficiency was lower than that in control group (P<0.01). Levels of IL-6, TNF-α, CysC, NPY, ET-1, vWF and Hcy were lower than those in control group (P<0.01). The total effect rate of disease in observation group was 94.74%, which was higher than 83.33% in control group (χ2=5.097, P<0.05). Conclusion: In addition to the therapy of routine western medicine, modified Qiju Dihuang Tang based on syndrome differentiation can control blood pressure of patients, relieve clinical symptoms, reduce maternal and infantile adverse outcomes, improve the comprehensive treatment effect, ameliorate vascular endothelial function and relieve injury caused by cytotoxic factor.关键词：hypertensive disorder complicating pregnancy;preeclampsia;Qiju Dihuang Tang;vascular endothelial function;serum cystatin C;neuropeptide;homocysteine8|6|3更新时间：2024-01-04
- 摘要：Objective: To discuss the clinical efficacy of Zhenwutang combined with Baoyuantang on type 2 cardio renal syndrome(CRS) and its effect on levels of serum nitric oxide (NO), endothelin-1 (ET-1), interleukin-1 (IL-1), IL-6 and tumor necrosis factor-α (TNF-α). Method: Totally 134 patients were randomly divided into control group (67 cases) and observation group (67 cases) by random number table. Patients in control group got irbesartan, 0.15 g/tmie, 1 time/day. Metoprolol tablet, 1.25 mg/time, 2 times/days. Spironolactone tablets, 20 mg/time, 1 time/day. Hydrochlorothiazide 25 mg/time, 1 time/day. digoxin tablets, 0.125-0.25 mg/day, when necessary. In addition to the therapy for control group, patients in observation group were also given Zhenwutang combined with Baoyuantang. The course of treatment was 3 months. Before and after treatment, cardiac function indexes, such as ventricular ejection fraction (LVEF), stroke volume (SV), N-natriuretic peptide (NT-proBNP), 6-minute walking test (6 MWT) and renal function index such as serum creatinine (Scr), urea nitrogen (BUN), serum cystatin C (CysC), urinary microscale albumin (mALB), urinary kidney injury molecule-1 (Kim-1) were recorded, glomerular filtration rate(eGFR) and UACR were calculated, and Lee's heart failure and traditional Chinese medicine(TCM)syndromes were scored. And levels of NO, ET-1, IL-6, IL-1 and TNF-α were detected. Result: The clinical efficacy in observation group was superior to that in control group by Ridit (P<0.05). And NYHA score and levels of NT-proBNP, Scr, BUN, CysC, mALB, Kim-1 and UACR were lower than those in control group (P<0.01), 6 MWT in observation group was longer than that in control group (P<0.01), and LVEF, SV and NO were higher than those in control group (P<0.05). Compared with before treatment, eGFR in observation group increased and was higher than that in control group(P<0.01). Scores of Lee's heart failure and TCM syndromes were lower than those in control group (P<0.01). And levels of ET-1, hs-CRP, IL-1, IL-6 and TNF-α were lower than those in control group (P<0.01). Conclusion: In addition to the routine western medicine therapy, Zhenwutang combined with Baoyuantang can improve heart function and renal function, relieve endothelial dysfunction and chronic inflammation, clinical symptoms, and improve the comprehensive treatment effect.关键词：type 2 cardio renal syndrome;Zhenwutang;Baoyuantang;endothelial dysfunction;chronic inflammation4|6|3更新时间：2024-01-04
- 摘要：Tetrastigma hemsleyanum is a peculiar precious herb of our country, and has been widely used in south China, especially in the Yangtze River basin. According to the traditional Chinese medicine, T. hemsleyanum has the effects in clearing heat and removing toxicity, circulating blood and relieving pain, and expelling wind and phlegm. On the basis of modern pharmacological studies, it has anti-tumor, anti-virus, immune regulation and other exact pharmacological effects. It was recorded firstly on Zhiwu Mingshi Tukao, which is a botany monograph written by Wu Qijun in the Qing dynasty, but its original plant was not clear. Until now, it has not been included in the Chinese Pharmacopoeia. Because modern scholars have rarely systematically studied on it, a series of key problems, such as the original plant primordial and the distribution of resources, have remained unclear. To solve them, this paper consulted 41 relevant literatures, summarized the origin and development of the plant, and determined the original plant of its plant was T. hemsleyanum of Tetrastigma from Vitaceae. This paper comprehensively described the different names of this herb, analyzed two possible reasons, and summarized the distribution of this herb. It is distributed mainly in all provinces in the south of the Yangtze River and most of the provinces through the Yangtze River, but rarely in the north of the Yangtze River. The paper suggested covering T. hemsleyanum within the scope of relevant administration, and including it in the Chinese Pharmacopoeia under the name of Sanyeqing. T. hemsleyanum is clearly identified as it sole original plant.关键词：Tetrastigma hemsleyanum;origin;resource;textual research6|6|5更新时间：2024-01-04
- 摘要：Lycii Fructus polysaccharide extracted from the fruit of Lycium chinense is one of the most active chemicals of Lycii Fructus, and has multiple pharmacological functions, such as enhancing immunity, anti-oxidation, anti-aging, inhibiting tumor, protecting retina, liver and nervous system, and sugar and lipid-decreasing effects. Many studies have demonstrated that Lycii Fructus polysaccharide was widely used to treat various diseases in clinic, such as tumor and immune diseases, hypertension and hyperlipidemia, acute liver injury, cerebral ischemia and apoplexy. Because Lycii Fructus polysaccharide has a significant efficacy in relieving central nervous system diseases, such as Alzheimer's disease and cerebral ischemia, its application in physical nervous system has attracted more and more attention. In recent years, studies on the neuroprotective effect of Lycii Fructus in central nervous system have been increased. To further promote the studies on the prevention and treatment of central nervous system diseases, this review systematically summarizes the neuroprotective effect of Lycii Fructus polysaccharide based on domestic and foreign reports for the effects of Lycii Fructus polysaccharide in alleviating neurodegenerative disease, protecting optic nerve, inhibiting oxidative stress, apoptosis and inflammatory response, reducing glutamate toxicity, and inhibiting the abnormal differentiation of nerve stem cells. However, because the neuroprotective mechanisms of Lycii Fructus Polysaccharide are complicated and not very clear in some aspects, we shall define the mechanisms of neuroprotective action of Lycii Fructus Polysaccharide with modern molecular biology techniques and gene analysis methods, which may provide useful theoretical support to further studies.关键词：Lycii Fructus polysaccharide;neuroprotective effect;mechanism of action;research progress9|8|7更新时间：2024-01-04
- 摘要：Morindae Officinalis Radix has a long history of medicinal use in China. It has a wide range of medicinal value and is one of the most commonly used traditional Chinese medicines in clinical application. According to modern studies, oligosaccharides are the main effective components in Morindae Officinalis Radix, with many functions such as anti-dementia, anti-depression, anti-oxidative stress, improving learning and memory ability, and enhancing immunity and sexual function. The above pharmacological actions are of great value for development and utilization. By consulting massive literature materials, it was found that many clinical effects of Morindae Officinalis Radix were correlated with the pharmacological actions of the oligosaccharides. These pharmacological actions have also scientifically interpreted the traditional functions of Morindae Officinalis Radix such as ‘tonifying kidney, dominating bone and benefiting brain’. Therefore, scholars both at home and abroad have done a lot of research work on it, especially the studies on the protective effects of the oligosaccharides from Morindae Officinalis Radix on nervous system. Relevant results showed that it had significant effects in anti-dementia, anti-depression, improving the learning and memory ability, with the potential to develop drugs for the treatment of neurodegenerative disease. In this paper, the researches of the past 20 years on extraction and purification process, component detection method and pharmacological actions of oligosaccharides from Morindae Officinalis Radix were collected and summarized upon accessing multiple databases such as CNKI, WanFang, PubMed, Science Direct and Springer. The researches and development prospects were also analyzed, and the research progress on oligosaccharides from Morindae Officinalis Radix was reviewed for further studying and comprehensive utilization of these oligosaccharides.关键词：Morindae Officinalis Radix;oligosaccharides;extraction and purification;component detection;pharmacological action5|7|4更新时间：2024-01-04
- 摘要：At present, anti-inflammatory drugs are used as major drugs only second to anti-infection drugs in the clinical treatment, including steroidal anti-inflammatory drugs, non-steroidal anti-inflammatory drugs and traditional Chinese medicine (TCM). Because many synthetic drugs have severe side effects, more and more attention has been paid to the development of anti-inflammatory drugs from natural drugs. Based on the overall syndrome differentiation-based thinking mode, TCM with unique effects and low side effects has attracted attention of inflammation researchers and clinicians around the world. Antipyretic and detoxifying TCM have a significant anti-inflammatory effect. In this paper, we reviewed the advance of studies on these herbs and their active constituents by referring to relevant literatures published in the past decade at home and abroad, and summarized the anti-inflammatory mechanisms. The anti-inflammatory mechanisms of antipyretic and detoxifying herbs could be analyzed in eight aspects, including the effects on vasoactive amines, arachidonic acid metabolites, inflammatory cytokines, inflammatory signaling pathway nuclear transcription factor-κB (NF-κB)and mitogen-activated protein kinase (MAPK), NO and chemokines, the removal of free radicals, and the adjustment of the hypothalamus-pituitary-adrenal cortex axis function. In addition, the current situations and existing problems were reviewed and analyzed, in order to screen out the antipyretic and detoxifying Chinese medicines and their active components, and further benefit clinical application.关键词：anti-inflammatory;antipyretic and detoxifying traditional Chinese medicine;mechanism;active ingredient;advance of studies4|6|23更新时间：2024-01-04
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