最新刊期
- “最新研究发现，绞股蓝皂苷L通过调控特定tRNA影响卵巢癌细胞铁死亡，为卵巢癌治疗提供新策略。”摘要：ObjectiveTo investigate the role of mature-tRNA-Asp-GTC and pre-tRNA-Arg-TCT in the ferroptosis phenotype of ovarian cancer （OC） cells and the regulatory mechanism of gypenoside L （Gyp-L） on mature-tRNA-Asp-GTC and pre-tRNA-Arg-TCT in OC cells.MethodsThe proliferation of human ovarian adenocarcinoma OVCAR3 cells was detected by cell counting kit-8 （CCK-8） assay， and the half-maximal inhibitory concentration （IC50） values of cisplatin （DDP）， Gyp-L， and DDP in the presence of Gyp-L were calculated to determine the intervention concentration for subsequent experiments. Cell cloning assay and scratch assay reflected the proliferation and migration ability of OVCAR3 cells. PANDORA-seq small RNA sequencing was used to detect the differentially expressed transfer RNA-derived small RNAs （tsRNAs） in the cells after Gyp-L intervention， and the corresponding target genes of the tsRNAs were found by the RNAhybrid software. Malondialdehyde （MDA）， glutathione （GSH）， and lipid peroxide （LPO） levels were measured by colorimetry or enzyme linked immunosorbent assay （ELISA） method， Fe2+ content by FerroOrange fluorescent probe， and reactive oxygen species （ROS） content by DCFH-DA fluorescent probe to reflect the occurrence of ferroptosis in OVCAR3 cells. OVCAR3 cells were divided into a control group， a 50 µmol·L-1 Gyp-L group， and a 100 µmol·L-1 Gyp-L group. Quantitative real-time polymerase chain reaction （PCR） was performed to detect the expression of mature-tRNA-Asp-GTC， mature-tRNA-Leu-CAA， mature-mt_tRNA-Tyr-GTA_5_end， mature-tRNA-Val-CAC， mature-mt_tRNA-Glu-TTC， pre-tRNA-Arg-TCT， mature-tRNA-Asn-GTT， hydroxymethylbilane synthase （HMBS）， Wnt， β-catenin， glutathione peroxidase 4 （GPX4）， Kelch-like ECH-associated protein 1 （KEAP1）， nuclear factor erythroid 2-related factor 2 （Nrf2）， activating transcription factor 3 （ATF3）， cystine/glutamate antiporter xCT， lysophosphatidylcholine acyltransferase 3 （LPCAT3）， and arachidonate 15-lipoxygenase （ALOX15）. Western blot was performed to detect the expression of HMBS， Wnt， β-catenin， GPX4， KEAP1， Nrf2， ATF3， xCT， LPCAT3， and ALOX15 proteins.ResultsThe 50 µmol·L-1 Gyp-L， 100 µmol·L-1 Gyp-L， DDP， 50 µmol·L-1 Gyp-L+DDP， and 100 µmol·L-1 Gyp-L+DDP groups showed significantly inhibited proliferation and migration of OVCAR3 cells （P<0.05） and exacerbated cell ferroptosis as reflected by the increase in the content of ROS， MDA， LPO， and Fe2+， as well as a decrease in the content of GSH （P<0.05）. Compared with the control group， Gyp-L effectively interfered with the expression of 25 tsRNAs in OVCAR3 cells （P<0.05， |log2Fc|>1）. Pre-tRNA-Arg-TCT/HMBS/WNT/β-catenin/GPX4， pre-tRNA-Arg-TCT/KEAP1/NRF2/xCT， mature-tRNA-Asp-GTC/ATF3/KEAP1/NRF2/xCT， and mature-tRNA-Asp-GTC/LPCAT3/ALOX15 axial expression was significantly aberrant after Gyp-L intervention （P<0.05）.ConclusionThe pre-tRNA-Arg-TCT/HMBS/Wnt/β-catenin/GPX4， pre-tRNA-Arg-TCT/KEAP1/Nrf2/xCT， mature-tRNA-Asp-GTC/ATF3/KEAP1/Nrf2/xCT， and mature-tRNA-Asp-GTC/LPCAT3/ALOX15 signaling pathways are involved in OC development. Gyp-L inhibits OC development by activating OVCAR3 cell ferroptosis onset mainly through the mature-tRNA-Asp-GTC/ATF3/KEAP1/Nrf2/xCT and mature-tRNA-Asp-GTC/LPCAT3/ALOX15 signaling axes.关键词：ovarian cancer;gypenoside L;mature-tRNA-Asp-GTC;pre-tRNA-Arg-TCT;ferroptosis0|0|0更新时间：2025-04-18
- “最新研究发现，绞股蓝皂苷L通过piR-hsa-2804461途径促进卵巢癌细胞凋亡，为卵巢癌治疗提供新思路。”摘要：ObjectiveTo explore the molecular mechanism by which gypenoside L （Gyp-L） promotes apoptosis and inhibits ovarian cancer （OC） through the FK506 binding protein（FKBP） prolyl isomerase 8 （FKBP8）/B-cell lymphoma-2 （Bcl-2） axis， using the piR-hsa-2804461 pathway as the entry point.MethodsThe effects of different concentrations of Gyp-L and cis-platinum on the proliferation activity of OVCAR3 cells were determined by the cell count kit-8（CCK-8） method to identify the appropriate intervention concentration for subsequent experiments. OVCAR3 cells were divided into Control， Gyp-L-L（50 µmol·L-1）， Gyp-L-H（100 µmol·L-1）， and cis-platinum（15 µmol·L-1） groups. The migration ability， clone formation ability， and apoptosis of OVCAR3 cells were detected by the cell scratch assay， cell clone assay， and flow cytometry， respectively. The mRNA expression levels of piR-hsa-2804461 and FKBP8/Bcl-2 axis-related genes in OVCAR3 cells were detected by Real-time polymerase chain reaction（Real-time PCR）， and the protein expression levels of FKBP8/Bcl-2 axis-related proteins were detected by Wes auto Real-time PCR mated Western blotting. Further， an OVCAR3 cell model with piR-hsa-2804461 knocked out was constructed. The cells were divided into blank， NC-inhibitor， inhibitor， NC-inhibitor+Gyp-L， and inhibitor+Gyp-L groups. The clone formation ability of OVCAR3 cells was detected by the cell clone assay， and the mRNA expression levels of piR-hsa-2804461 and FKBP8/Bcl-2 axis-related genes and the protein expression levels of FKBP8/Bcl-2 axis-related proteins were detected by Real-time PCR and Wes automated Western blotting， respectively.ResultsGyp-L significantly inhibited the migration and proliferation of OVCAR3 cells （P<0.01）， promoted apoptosis of OVCAR3 cells （P<0.05）， and increased the mRNA expression level of piR-hsa-2804461 in OVCAR3 cells （P < 0.05）， while reducing the mRNA and protein expression levels of FKBP8 and Bcl-2 （P < 0.05）， and increasing the mRNA and protein expression levels of Bax， Caspase-3， and Caspase-9， which are related to the FKBP8/Bcl-2 axis （P < 0.05）.ConclusionGyp-L may promote apoptosis and affect the occurrence of ovarian cancer by regulating the piR-hsa-2804461/FKBP8/Bcl-2 axis.关键词：ovarian cancer;gypenoside L;piR-hsa-2804461;FKBP8;B-cell lymphoma-2 （Bcl-2）4|0|0更新时间：2025-04-18
- “最新研究发现，绞股蓝皂苷L可通过EGFR/STAT3/HK2通路抑制动脉粥样硬化和卵巢癌的发生发展，为中医药共治这两种疾病提供新思路。”摘要：ObjectiveWith the epidermal growth factor receptor（EGFR）/Signal Transducers and Activators of Transcription（STAT3）/Hexokinase 2（HK2） signaling pathway in atherosclerosis （AS） and ovarian cancer （OC） as the entry point， this paper discusses the molecular mechanism of Gypenoside L （Gyp-L） treating AS and OC with different diseases， provides a new perspective and theoretical basis for TCM treating AS and OC with EGFR-STAT3-HK2 pathway， and enriches the scientific connotation of the theory of "cytoskeleton in the heart".MethodsCCK-8 was used to detect the proliferation of HUVEC and OVCAR-3 cells， in order to determine the intervention concentration for subsequent experiments. The colorimetric method was used to detect the NO content in HUVEC and the contents of pyruvate and LDH in two cell lines. Cell cloning experiments and scratch experiments reflect the proliferation and migration ability of OVCAR-3 cells. Western blot was used to detect the expression levels of relevant proteins. Furthermore， two cell models overexpressing EGFR were constructed and co treated with Gyp-L. HUVEC cells were divided into control， ox-LDL， OE-NC， OE-EGFR， OE-NC+Gyp-L， and OE-EGFR+Gyp-L group. OVCAR-3 cells were divided into control， OE-NC， OE-EGFR ， OE-NC+Gyp-L， and OE-EGFR+Gyp-L group. The colorimetric method was used to detect the NO content in HUVEC and the contents of pyruvate and LDH in two cell lines. Western blot was used to detect the expression levels of EGFR-STAT3-HK2 pathway related proteins. Cell cloning experiments and scratch experiments reflect the proliferation and migration ability of OVCAR-3 cells.ResultsGyp-L can significantly reduce the NO content of HUVEC and the pyruvate and LDH content of two cell lines （P<0.05）； Inhibit the proliferation and migration ability of OVCAR-3 cells； Reduce the expression levels of EGFR/STAT3/HK2 pathway related proteins in HUVEC and OVCAR-3 cell lines （P<0.05）， and inhibit the glycolysis pathway.ConclusionGyp-L can inhibit glycolysis in HUVEC and OVCAR-3 cells through the EGFR/STAT3/HK2 pathway，thereby suppressing the occurrence and development of AS and OC.关键词：atherosclerosis;ovarian cancer;gypenoside L;epidermal growth factor receptor（EGFR）/signal transducers and activators of transcription（STAT3）/hexokinase 2（HK2） signaling pathway;glycolysis4|0|0更新时间：2025-04-18
- “最新研究发现，绞股蓝皂苷L通过抑制卵巢癌细胞糖酵解途径，有效降低癌细胞增殖和迁移能力，为卵巢癌治疗提供新思路。”摘要：ObjectiveTo explore the molecular mechanism of gypenoside L （Gyp-L） in the treatment of ovarian cancer （OC） by taking the glycolytic pathway of OC as the key point.MethodsThe proliferation activity of OVCAR3 cells was measured by the cell counting kit-8 （CCK-8） assay to determine the appropriate intervention concentration for subsequent experiments. The cell clone formation assay and the scratch healing assay were employed to assess the proliferation and migration capabilities of OVCAR3 cells. OVCAR3 cells were divided into a blank group， a Gyp-L-L group （low concentration of Gyp-L， 50 µmol·L-1）， a Gyp-L-H group （high concentration of Gyp-L， 100 µmol·L-1）， and a cisplatin （15 µmol·L-1） group. The glucose consumption in OC cells was determined using a kit， and the expression levels of related mRNAs in OVCAR3 cells were detected by real-time fluorescence quantitative polymerase chain reaction （Real-time PCR）. The expressions of related proteins were detected by Wes automatic Western blot quantitative analysis.ResultsValidated by the cell scratch assay， the scratch healing rate of OVCAR3 cells was decreased after Gyp-L intervention， reflecting that Gyp-L could significantly inhibit the migration of OVCAR3 cells （P<0.05）. According to the clone formation assay， the cell colony formation ability of the Gyp-L-L group， Gyp-L-H group， and cisplatin group was significantly lower than that of the control group （P<0.05）. In OVCAR3 cells， compared with those in the control group， the levels of glucose consumption and lactate generation in the Gyp-L-L group and Gyp-L-H group were significantly reduced （P<0.05）， indicating that Gyp-L could effectively inhibit the glycolysis level of OC cells. Meanwhile， Gyp-L could suppress the expression levels of glucokinase （GCK）， phosphofructokinase liver （PFKL）， signal transducer and activator of transcription 3 （STAT3）， lactate dehydrogenase D （LDHD）， phosphoglycerate mutase 1 （PGAM1）， mRNAs， and proteins related to the glycolytic pathway in OC cells.ConclusionGyp-L may inhibit the occurrence and development of OC by influencing the GCK-mediated glycolytic pathway.关键词：ovarian cancer;gypenoside L;glucokinase （GCK）;glycolysis;cell proliferation2|0|0更新时间：2025-04-18
- “经典名方防己茯苓汤研究进展，专家系统考证其组成、剂量等关键信息，为复方制剂研发提供文献依据。”摘要：The classic formula Fangji Fulingtang is from ZHANG Zhongjing's Synopsis of the Golden Chamber in the Eastern Han dynasty. It is composed of Stephaniae Tetrandrae Radix， Astragali Radix， Cinnamomi Ramulus， Poria， and Glycyrrhizae Radix et Rhizoma， with the effects of reinforcing Qi and invigorating spleen， warming Yang and promoting urination. By a review of ancient medical books， this paper summarizes the composition， original plants， processing， dosage， decocting methods， indications and other key information of Fangji Fulingtang， aiming to provide a literature basis for the research， development， and clinical application of preparations based on this formula. Synonyms of Fangji Fulingtang exist in ancient medical books， while the formula composition in the Synopsis of the Golden Chamber is more widespread and far-reaching. In this formula， Stephaniae Tetrandrae Radix， Astragali Radix， Cinnamomi Ramulus， Poria， and Glycyrrhizae Radix et Rhizoma are the dried root of Stephania tetrandra， the dried root of Astragalus embranaceus var. mongholicus， the dried shoot of Cinnamomum cassia， the dried sclerotium of Poria cocos， and the dried root and rhizome of Glycyrrhiza uralensis， respectively. Fangji Fulingtang is mainly produced into powder， with the dosage and decocting method used in the past dynasties basically following the original formula. Each bag is composed of Stephaniae Tetrandrae Radix 13.80 g， Astragali Radix 13.80 g， Cinnamomi Ramulus 13.80 g， Poria 27.60 g， and Glycyrrhizae Radix et Rhizoma 9.20 g. The raw materials are purified， decocted in water from 1 200 mL to 400 mL， and the decoction should be taken warm， 3 times a day. Fangji Fulingtang was originally designed for treating skin edema， and then it was used to treat impediment in the Qing dynasty. In modern times， it is mostly used to treat musculoskeletal and connective tissue diseases and circulatory system diseases， demonstrating definite effects on various types of edema and heart failure. This paper clarifies the inheritance of Fangji Fulingtang and reveals its key information （attached to the end of this paper）， aiming to provide a theoretical basis for the development of preparations based on this formula.关键词：classic formula;Fangji Fulingtang;ancient and modern application;key information;textual research4|0|0更新时间：2025-04-18
- “据最新研究，专家全面分析了中药肾损伤的毒理机制和减毒措施，为中药安全性研究提供新思路。”摘要：ObjectiveWe reviewed the existing experimental studies about renal injury-inducing Chinese medicine and systematically analyzed the toxicity mechanisms， toxic components， toxicity attenuation measures， and modern evaluation methods of renal injury-inducing Chinese medicine. The results are expected to provide new ideas for the modern research on kidney injury-inducing Chinese medicine， offer new breakthrough points for the toxicity attenuation of Chinese medicine by compatibility and processing， and give insights into the future research of Chinese medicine toxicology on the basis of ensuring the safety and scientific application of Chinese medicine.MethodsThe animal， cell， and clinical studies of kidney injury-inducing Chinese medicine were retrieved from CNKI， Wanfang Data， VIP， PubMed， and Web of Science. The names and toxic components of renal injury-inducing Chinese medicine， renal injury sites， toxicity mechanisms， toxicity attenuation measures， and related evaluation methods were summarized.ResultsThe toxicity mechanisms of kidney injury-inducing Chinese medicine mainly involved oxidative stress， endoplasmic reticulum stress， inflammatory cell infiltration， and organic anion transporters. Processing and compatibility were the main toxicity attenuation measures. The evaluation methods encompassed animal experiments， cell models， network pharmacology， metabolomics， toxicology genomics， and fluorescent probe technology.ConclusionAt present， the toxicological verification of kidney injury-inducing Chinese medicine starts from toxic components and combines various experimental methods， which is more comprehensive and systematic than the previous studies based on only animal experiments. According to the classical theories of traditional Chinese medicine， the toxicity of kidney injury-inducing Chinese medicine is mainly attenuated by decocting in water， steaming， and frying. With the progress of science and technology， new processing methods for toxicity attenuation are emerging， and structural transformation， fermentation， and microwave methods are the key research directions of toxicity attenuation of Chinese medicine in recent years.关键词：renal injury-inducing Chinese medicine;toxic components;occurrence mechanism;toxicity attenuation measures;evaluation method4|0|0更新时间：2025-04-18
- “最新研究揭示地榆-槐花配伍治疗溃疡性结肠炎的潜在机制，通过网络药理学和分子对接技术，发现其可能通过影响PI3K/Akt通路抑制病理性血管增生。”摘要：ObjectiveTo explore the potential mechanism of action of the combination of Sanguisorbae Radix and Sophorae Flos （DH） in the treatment of ulcerative colitis （UC） using network pharmacology methods and molecular docking technology.MethodsNetwork pharmacology analysis was utilized to predict the potential targets of DH for the treatment of UC. The therapeutic effects were experimentally validated by inducing a UC model in mice with 3% dextran sulfate sodium （DSS）. The experimental groups were the normal group， the model group， the salazosulfapyridine group （100 mg·kg-1）， and the low， medium， and high dose groups of DH （1.2， 2.4， and 4.8 g·kg-1）. The efficacy of the treatment was assessed through the general condition of the mice， histopathological examination， and the expression levels of inflammatory markers in the colon. The effect of DH on angiogenesis was explored by messenger RNA （mRNA） detection of colonic angiogenesis-related mediators， vascular endothelial growth factor （VEGF） immunohistochemistry， microvessel density （MVD） detection， and transmission electron microscopy. The phosphatidylinositol-3-kinase （PI3K）-protein kinase B （Akt） signaling pathway proteins were quantitatively analyzed through Western blot to assess whether the suppression of pathological angiogenesis by DH is associated with this pathway.ResultsNetwork pharmacological analysis yielded 112 potential core therapeutic targets for the treatment of UC with DH， of which the core targets were tumor protein 53 （TP53）， JUN， interleukin （IL）-6， Akt1， and tumor necrosis factor （TNF）. Compared with the normal group， mice in the model group showed significant weight loss， colon shortening， and high DAI score， increased expression of inflammatory factors IL-6， IL-1β， and TNF-α， as well as increased mRNA expression levels of angiogenesis-related mediators VEGF， vascular cell adhesion molecule 1 （VCAM1）， angiotensin 1 （Ang1）， matrix metalloproteinase （MMP）-1， MMP-2， and MMP-9. The positive expression of CD31 and VEGF in colonic tissue increased， and the protein expression of the PI3K/Akt pathway was increased （P<0.05）. The endothelial cells of the colonic mucosa and the colonic vasculature were severely damaged. Compared with the model group， mice in the DH groups had significantly reduced weight loss and colon shortening， lower DAI scores， and a significant decrease in mRNA expression of inflammatory factors and angiogenesis-related mediators. In addition， there was decreased positive expression of CD31 and VEGF in colonic tissue and decreased protein expression of the PI3K-Akt pathway （P<0.05）.ConclusionNetwork pharmacology， molecular docking， and experimental validation are applied to explore the mechanism of action of DH in the treatment of UC， and it is found that DH is able to improve the symptoms of colitis and inhibit the pathological angiogenesis in UC mice. Its action might be related to affecting the PI3K/Akt pathway.关键词：Sanguisorbae Radix;Sophorae Flos;network pharmacology;ulcerative colitis;angiogenesis4|0|0更新时间：2025-04-18
- “化浊解毒方通过调控线粒体自噬治疗溃疡性结肠炎，改善肠道黏膜损伤，为UC治疗提供新方案。”摘要：ObjectiveTo investigate the mechanism of Huazhuo Jiedu prescription in treating ulcerative colitis （UC） by regulating mitophagy.MethodsThe genes related to mitophagy and UC were retrieved from GeneCards， and then the common genes of mitophagy and UC were analyzed by metascape to identify the genes related to mitophagy in UC. Animal experiments were carried out to decipher the mechanism by which Huazhuo Jiedu prescription treated UC by regulating mitophagy. Sixty C57BL/6 male mice were randomized into normal， model， high-， medium-， and low-dose （50， 25， and 12.5 g·kg-1， respectively） Huazhuo Jiedu prescription， and mesalazine （0.52 g·kg-1·d-1） groups， with 10 mice in each group. After successful modeling by the dextran sulfate sodium-free drinking method， the colonic mucosal damage was observed by hematoxylin-eosin staining， and the ultracellular structure of colon mucosa was observed by transmission electron microscopy. The expression levels of mitophagy-related proteins PTEN-induced putative kinase 1 （Pink1） and E3 ubiquitin ligase （Parkin） were determined by Western blot. The expression of prohibitin 2 （PHB2）， ubiquitin-specific protease 15 （USP15）， ubiquitin-specific protease 30 （USP30） in the colon tissue was detected by immunofluorescence （IF）.ResultsAll the drug intervention groups showed ameliorated pathological manifestations of the colonic mucosa and improved mitochondrial structures in UC mice. Compared with the normal group， the model group demonstrated up-regulated protein levels of Pink1 and Parkin （P<0.05）， enhanced average fluorescence intensity of PHB2 （P<0.05）， and weakened average fluorescence intensity of USP15 and USP30 （P<0.05）. Compared with the model group， the mesalazine group and the high- and medium-dose Huazhuo Jiedu prescription groups showcased down-regulated protein levels of Pink1 and Parkin （P<0.05）， decreased average fluorescence intensity of PHB2 （P<0.05）， and enhanced average fluorescence intensity of USP15 and USP30 （P<0.05）. The low-dose Huazhuo Jiedu prescription group showed down-regulated protein levels of Pink1 and Parkin （P<0.05）， weakened average fluorescence intensity of PHB2 （P<0.05）， and enhanced average fluorescence intensity of USP15 and USP30 （P<0.05）.ConclusionHuazhuo Jiedu prescription can attenuate the intestinal mucosal injury and improve the mitochondrial cell ultrastructure in UC mice by regulating the expression of Pink1-Parkin pathway and inhibiting excessive mitophagy.关键词：Huazhuo Jiedu prescription;ulcerative colitis;mitophagy;PTEN-induced putative kinase 1 （PINK1）;E3 ubiquitin ligase （Parkin）2|0|0更新时间：2025-04-18
- “最新研究发现，补中益气汤能有效防治运动性疲劳，可能通过调节PINK1相关信号通路，改善骨骼肌线粒体稳态。”摘要：ObjectiveTo explore the effect of Buzhong Yiqitang on exercise-induced fatigue and its potential mechanism.MethodsSixty male SPF-grade C57BL/6J mice were randomized into blank， model， low-， medium-， high-dose （4.1， 8.2， and 16.4 g·kg-1， respectively） Buzhong Yiqitang， and vitamin C （0.04 g·kg-1） groups. The blank and model groups were administrated with normal saline. Each group was administrated with corresponding agents by gavage at a dose of 0.2 mL once a day. Except the blank group， other groups underwent a 6-weeks exhaustive swimming test under negative gravity. At the end of the experiment， blood was collected， and the thymus， spleen， liver， and kidney weights were measured. Serum levels of lactic acid （LD）， blood urea nitrogen （BUN）， creatine kinase （CK）， and malondialdehyde （MDA） were assessed by kits to evaluate fatigue. Hematoxylin-eosin staining was performed to observe pathological changes in the skeletal muscle. Electron microscopy was used to examine the skeletal muscle cell ultrastructure， with a focus on mitochondrial morphological changes. The adenosine triphosphate （ATP） content and activities of mitochondrial respiratory chain complexes Ⅰ， Ⅱ， and Ⅴ in skeletal muscle were determined by kits. The expression levels of key genes and proteins in the PTEN-induced putative kinase 1 （PINK1）-mediated mitochondrial homeostasis pathways in the skeletal muscle were evaluated via Real-time PCR and Western blot， respectively.ResultsCompared with the blank group， the model group showed reductions in weight gain rate （P<0.01） and thymus index （P<0.01）， rises in serum levels of LD， BUN， MDA， and CK （P<0.01）， disarrangement of skeletal muscle， broken muscle fibers， inflammatory cell infiltration in muscle fiber gaps， abnormal morphological changes （increased vacuolated mitochondria and disappearance of cristae） of mitochondria in skeletal muscle cells， and decreased mitochondria. In addition， the skeletal muscle in the model group showed reduced content of ATP， weakened activities of mitochondrial respiratory chain complexes Ⅰ， Ⅱ， and Ⅴ （P<0.05）， up-regulated mRNA levels of PINK1， E3 ubiquitin-protein ligase （Parkin）， hairy/enhancer-of-split related with YRPW motif 1 （HEY1）， dynamin-related protein 1 （Drp1）， sequestosome 1 （p62）， and hypoxia-inducible factor 1 alpha （HIF-1α） （P<0.05）， and down-regulated protein level of microtubule-associated protein 1-light chain 3B （LC3B） （P<0.01）. Compared with the model group， Buzhong Yiqitang prolonged the swimming exhaustion time （P<0.01）， increased the weight gain rate （P<0.01） and thymus index （P<0.01）， lowered the serum levels of LD， BUN， MDA， and CK （P<0.05， P<0.01）. The skeletal muscle in the Buzhong Yiqitang groups showed neat arrangement， reduced inflammatory cells， intact mitochondria with dense cristae， and increased mitochondria. In addition， the skeletal muscle in the Buzhong Yiqitang groups showcased increased ATP content， enhanced activities of mitochondrial respiratory chain complexes Ⅰ， Ⅱ， and Ⅴ （P<0.05， P<0.01）， up-regulated protein levels of PINK1， Parkin， HEY1， LC3B， and Drp1 and mRNA level of HIF-1α （P<0.05， P<0.01）， and down-regulated expression level of p62 （P<0.05， P<0.01）.ConclusionBuzhong Yiqitang can prevent and treat exercise-induced fatigue by regulating the mitochondrial homeostasis of skeletal muscle via the HIF-1α/PINK1/Parkin and HIF-1α/HEY1/PINK1 signaling pathways.关键词：exercise-induced fatigue;Buzhong Yiqitang;PTEN-induced putative kinase 1 （PINK1）;mitochondrial homeostasis of skeletal muscle;mitochondrial autophagy2|0|0更新时间：2025-04-18
- “最新研究发现，健脾活骨方通过调节5-LO-PPARγ信号轴改善氧化脂质代谢物水平，有效治疗大鼠激素性股骨头坏死。”摘要：ObjectiveTo unveil the mechanism of Jianpi Huogu prescription （JPHGP） in ameliorating the dyslipidemia of steroid-induced osteonecrosis of the femur head （SONFH） by oxylipidomics combined with transcriptomics.MethodsSixty SD rats were assigned into normal， model， low-， medium-， and high-dose （2.5， 5， and 10 g·kg-1， respectively） JPHGP， and Jiangushengwan JGSW （1.53 g·kg-1） groups. Lipopolysaccharide was injected into the tail vein at a dose of 20 μg·kg-1 on days 1 and 2， and methylprednisolone sodium succinate was injected at a dose of 40 mg·kg-1 into the buttock muscle on days 3 to 5. The normal group received an equal volume of normal saline. Drug administration by gavage began 4 weeks after the last injection， and samples were taken after administration for 8 weeks. Hematoxylin-eosin staining was conducted to reveal the histopathological changes of the femoral head， and the number of adipocytes， the rate of empty bone lacunae， and the trabecular area were calculated. Micro-computed tomography was used for revealing the histological and histomorphometrical changes of the femoral head. Enzyme-linked immunosorbent assay was employed to measure the serum levels of triglyceride （TG）， total cholesterol （TC）， low-density lipoprotein （LDL）， high-density lipoprotein （HDL）， apolipoprotein A1 （ApoA1）， and apolipoprotein B （ApoB）. At the same time， the femoral head was collected for oxylipidomic and transcriptomic detection. The differential metabolites and differential genes were enriched and analyzed， and the target genes regulating lipid metabolism were predicted. The predicted target proteins were further verified by molecular docking， immunohistochemistry， and Western blot.ResultsCompared with the normal group， the model group showcased thinning of the femoral head， trabecular fracture， karyopyknosis， subchondral cystic degeneration， increases in the number of adipocytes and the rate of empty bone lacunae （P<0.01）， a reduction in the trabecular area （P<0.01）， decreases in BMD， Tb.Th， Tb.N， and BV/TV， and increases in Tb.Sp and BS/BV （P<0.01）. Compared with the model group， the JPHGP groups showed no obvious thinning of the femoral head or subchondroidal cystic degeneration. The high- and medium-dose JPHGP groups presented declines in the number of adipocytes and the rate of empty bone lacunae， an increase in the trabecular area （P<0.05， P<0.01）， rises in MD， Tb.Th， Tb.N， and BV/TV， and decreases in Tb.Sp and BS/BV （P<0.05， P<0.01）. Compared with the normal group， the model group showcased raised serum levels of TG， TC， LDL， and ApoB and lowered serum levels of HDL and ApoA1 （P<0.01）. Compared with the model group， the JPHGP groups had lowered serum levels of TG， TC， LDL， and ApoB （P<0.05， P<0.01） and a risen serum level of ApoA1 （P<0.05， P<0.01）. Moreover， the serum level of HDL in the high-dose JPHGP group increased （P<0.01）. A total of 19 different metabolites of disease set and drug set were screened out by oxylipidomics of the femoral head， and 119 core genes with restored expression were detected by transcriptomics. The enriched pathways were mainly concentrated in inflammation， lipids， apoptosis， and osteoclast differentiation. Molecular docking， immunohistochemistry， and Western blot results showed that compared with the normal group， the model group displayed increased content of 5-lipoxygenase （5-LO） and peroxisome proliferator-activated receptor γ （PPARγ） in the femoral head （P<0.01）. Compared with the model group， medium- and high-dose JPHGP reduced the content of 5-LO and PPARγ （P<0.05， P<0.01）.ConclusionJPHGP can restore the levels of oxidized lipid metabolites by regulating the 5-LO-PPARγ axis to treat SONFH in rats. This study provides an experimental basis for JPHGP treatment of SONFH from the spleen.关键词：steroid-induced osteonecrosis of the femoral head;Jianpi Huogu prescription;blood lipids;oxylipidomics;transcriptomics4|0|0更新时间：2025-04-18
- “据最新研究报道，地黄宝源颗粒和茯苓运化颗粒序贯给药对2型糖尿病小鼠具有显著降糖效果，并能改善糖尿病视网膜病变。”摘要：ObjectiveTo investigate the therapeutic effect of sequential administration of Dihuang Baoyuan granules （DHBY， the prescription for consolidating body resistance） and Fuling Yunhua granules （FLYH， the prescription for treating symptoms） on spontaneous type 2 diabetes mellitus （T2DM） in mice.MethodsAccording to the fasting blood glucose （FBG） level， 12-week-old db/db mice were randomized into six groups： model， DHBY （18.02 g·kg-1）， FLYH （14.80 g·kg-1）， sequential administration 1 （SEQ-1， DHBY 18.02 g·kg-1+FLYH 14.80 g·kg-1）， sequential administration 2 （SEQ-2， FLYH 14.80 g·kg-1+DHBY 18.02 g·kg-1）， and dapagliflozin （Dapa， 1.3 mg·kg-1）. The m/m mice in the same litter were selected as the normal group. The mice were administrated with corresponding drugs by gavage for 8 consecutive weeks. During the 8 weeks of drug administration and 2 weeks after withdrawal， the retinal thickness， FBG， hemoglobin A1c （HbA1c）， and insulin were determined， and histopathological changes of the pancreas， liver， kidney， and retina were observed by hematoxylin-eosin （HE） staining.ResultsCompared with the model group， SEQ-1 for 4 weeks lowered the FBG level （P<0.05）， raised the insulin level， decreased the triglyceride （TG） level （P<0.05）， increased the number of optic ganglion cells and diminished vacuolar degeneration of pancreatic islet and liver. SEQ-2 lowered FBG and HbA1c levels （P<0.05）， rose the insulin level， increased the retinal thickness and the number of optic ganglion cells （P<0.05）， and alleviated vacuolar degeneration of pancreatic islet and liver. Two weeks after drug withdrawal， Dapa tended to increase FBG and HbA1c compared with those at the time of drug withdrawal. However， the levels of FBG and HbA1c in the SEQ-2 group remained decreasing （P<0.05）.ConclusionSEQ-1 and SEQ-2 can lower the blood glucose level and ameliorate diabetic retinopathy， and SEQ-2 outperformed DHBY and FLYH in lowering the blood glucose level. Moreover， SEQ-2 can maintain the blood glucose-lowering effect after drug withdrawal.关键词：Dihuang Baoyuan granules;Fuling Yunhua granules;type 2 diabetes mellitus;diabetic retinopathy;sequential administration4|0|0更新时间：2025-04-18
- “据最新研究报道，线粒体自噬在肾纤维化中发挥关键作用，中药单体及复方制剂通过调控相关信号通路，有效干预肾纤维化进程。”摘要：With the main pathological features of glomerulosclerosis and interstitial fibrosis， renal fibrosis is a key pathological process causing chronic kidney disease to progress to end-stage disease. As a cellular autophagic process， mitochondrial autophagy plays a crucial role in maintaining mitochondrial mass and functional stability. Mitochondrial dysfunction is considered to be one of the key factors driving the progression of fibrosis. Phosphatase and tension protein homologue （PTEN） induce various signalling pathways such as putative kinase 1/parkin， Nip3-like protein X/Bcl-2 interacting protein 3， and FUN14 structural domain-containing protein 1 to activate mitochondrial autophagy to participate in the regulation of fibrogenic factors， amelioration of oxidative stress， and inhibition of inflammatory response and apoptosis， which in turn effectively slows down the progression of renal fibrosis. Studies have shown that traditional Chinese medicine monomers and compound preparations， including phenolics， terpenoids， ketones， and alkaloids， can regulate mitochondrial autophagy-related signalling pathways and achieve significant clinical efficacy in intervening in the progression of renal fibrosis for the treatment of chronic kidney disease. This paper summarized the mechanism of mitochondrial autophagy and the research progress of traditional Chinese medicine intervention in renal fibrosis to provide new ideas for the study of the mechanism of traditional Chinese medicine in treating renal fibrosis.关键词：mitochondrial autophagy;renal fibrosis;mechanism of action;traditional Chinese medicine;research progress4|0|0更新时间：2025-04-18
- “最新研究发现，益经汤通过调节TLR4/MyD88/NF-κB信号通路，减轻卵巢炎症反应，改善卵巢储备功能减退大鼠卵巢功能。”摘要：ObjectiveTo investigate the effect and mechanism of Yijingtang （YJT） in treating diminished ovarian reserve （DOR） in rats by regulating the Toll-like receptor 4 （TLR4）/myeloid differentiation factor 88 （MyD88）/nuclear factor-κB （NF-κB） signaling pathway.MethodsFifty female SD rats with normal estrous cycles were randomly allocated into blank， model， low- and high-dose （12.579 and 25.158 g·kg-1， respectively） YJT， and dehydroepiandrosterone （7.487 5 mg·kg-1） groups， with 10 rats in each group. The rats in other groups except the blank group were administrated with the tripterygium glycosides tablet suspension （5 mg·kg-1） by gavage for 14 days for the modeling of DOR. The rats in the drug treatment groups were administrated with corresponding drugs by gavage from day 15 for 30 consecutive days， and those in the blank and model groups received equal volumes of distilled water. The vaginal exfoliated cell smears were observed to assess the changes in the estrous cycle. The wet weight of bilateral ovaries was weighed for calculation of the ovarian index. Hematoxylin-eosin staining was performed to observe the histopathological changes in the ovaries and the proportions of follicles at various levels were calculated. The serum levels of sex hormones ［follicle-stimulating hormone （FSH）， luteinizing hormone （LH）， estradiol （E2）， and anti-Müllerian hormone （AMH）］ and inflammatory factors ［tumor necrosis factor-alpha （TNF-α）， interleukin-1beta （IL-1β）， and interleukin-10 （IL-10）］ were determined by enzyme-linked immunosorbent assay. Real-time quantitative polymerase chain reaction（Real-time PCR） was conducted to determine the mRNA levels of TLR4， MyD88， IκBα， NF-κB and inflammatory factors in the ovarian tissue. Western blot was employed to measure the protein levels of factors related to the TLR4/MyD88/NF-κB signaling pathway in the ovarian tissue. Immunofluorescence （IF） was used to detect the nuclear translocation of NF-κB p65 in the ovarian tissue.ResultsCompared with the blank group， the model group showed disturbed estrous cycles， increased inflammatory infiltration in the ovarian tissue， decreases in ovarian index and proportion of presinusoidal follicles， and an increase in the proportion of atretic follicles （P<0.05， P<0.01）. In addition， the model group showed elevated serum levels of FSH， LH， TNF-α， and IL-1β， up-regulated mRNA levels of TLR4， MyD88， IκBα， NF-κB， TNF-α， and IL-1β and protein levels of TLR4， MyD88， p-IκBα， and p-NF-κB p65 （P<0.01）， lowered serum levels of AMH， E2， and IL-10， down-regulated mRNA level of IL-10 （P<0.01）， and massive nuclear translocation of NF-κB p65 in the ovarian tissue. Compared with the model group， dehydroepiandrosterone and low and high doses of YJT restored the disturbed estrous cycle， reduced inflammatory infiltration in the ovarian tissue， increased the ovarian index （P<0.01）， and changed the follicular composition ratio （P<0.01）. Furthermore， the drugs lowered the serum levels of FSH， LH， TNF-α， and IL-1β， down-regulated the mRNA levels of TLR4， MyD88， IκBα， NF-κB， TNF-α， and IL-1β and the protein levels of TLR4， MyD88， p-IκBα， and p-NF-κB p65 （P<0.05， P<0.01）， raised the serum levels of AMH， E2， and IL-10， up-regulated the mRNA level of IL-10 （P<0.05， P<0.01）， and reduced the nuclear translocation of NF-κB p65 in the ovarian tissue.ConclusionYJT may inhibit the release and expression of inflammatory factors by regulating the TLR4/MyD88/NF-κB signaling pathway to attenuate the inflammatory responses in the ovarian tissue， thereby improving the ovarian function in DOR rats.关键词：Yijingtang;diminished ovarian reserve;inflammatory response;Toll-like receptor 4 （TLR4）;myeloid differentiation factor 88 （MyD88）;nuclear factor-κB （NF-κB）4|0|0更新时间：2025-04-18
- “据最新报道，红云制药（贵州）有限公司的益肺止咳胶囊，经专家循证医学方法制定的《共识》明确了其临床应用规范，为合理用药提供科学依据。”摘要：As an exclusive Miao medicine of Honwing Pharma （Guizhou） Co.， Ltd.， Yifei Zhike capsules are both a prescription drug and an over-the-counter （OTC） drug. Its main ingredients include Ranunculus ternatus and Panax notoginseng. With the effects of nourishing Yin and moistening the lungs， as well as relieving cough and reducing phlegm， Yifei Zhike capsules are often used in the treatment of acute and chronic bronchitis， pulmonary tuberculosis， and other diseases. However， there is insufficient understanding of their efficacy， suitable syndromes， and safety in clinical practice， with a lack of relevant expert consensus on clinical application. To standardize their clinical application， 30 experts from the fields of respiratory medicine， pharmacy， and evidence-based medicine were invited to develop an Expert Consensus on the Clinical Application of Yifei Zhike Capsules （Consensus for short） through evidence-based medicine methods. The Consensus clarified the syndrome characteristics， disease stages， dosages， treatment courses， combined medication， and other norms in the treatment of acute/chronic bronchitis and pulmonary tuberculosis and could be applicable to clinical physicians and pharmacists in medical and health institutions at all levels. In disease diagnosis， it provided diagnostic criteria for traditional Chinese medicine and Western medicine and clarified that the suitable traditional Chinese medicine syndrome was the syndrome of Qi-Yin deficiency with intermingled phlegm-blood stasis. Clinical studies have confirmed that Yifei Zhike capsules combined with standard anti-tuberculosis therapy can effectively improve the symptoms of pulmonary tuberculosis patients， increase the sputum smear conversion rate， and promote the absorption of lesions. When treating acute cough caused by respiratory tract infections， Yifei Zhike capsules can increase the markedly effective rate and the seven-day disappearance rate of cough symptoms. Meanwhile， recommendations for specific usage， dosages， and treatment courses were given for different diseases， and it was pointed out that long-term medication required key monitoring of adverse reactions. In safety， the adverse reactions of Yifei Zhike capsules involved multiple aspects such as the digestive system and allergic reactions， and pregnant women and women during menstruation were prohibited from using it. In addition， modern research has shown that Yifei Zhike capsules have an adjuvant therapeutic effect on tuberculous pleurisy and may be effective for inflammatory and benign pulmonary nodules. However， further research should be conducted on the toxicological safety of long-term medication. The formulation of the Consensus provides a scientific basis for the rational clinical application of Yifei Zhike capsules， which helps to improve clinical efficacy and reduce medication risks.关键词：expert consensus;Yifei Zhike capsules;respiration;clinical application;Miao medicine4|0|0更新时间：2025-04-17
- “最新研究揭示抑郁症患者大脑神经递质表达态势，为异质性病因研究提供新思路，验证“大脑多神经递质振荡失衡”假说。”摘要：ObjectiveThe purpose of the study was to analyze on the characteristic expression patterns of six neurotransmitters，namely serotonin （5-HT），dopamine （DA），acetylcholine （ACh），norepinephrine （NE），inhibitory neurotransmitter （INH），and excitatory neurotransmitter （EXC），in the whole-brain and different brain regions from depression populations by system software of Search of Encephalo Telex （SET），and providing new ideas for the study of heterogeneous etiology of depression.Methods1. A retrospective study on supra-slow signals of EEG fluctuation in 1028 cases of depression，the expression information of six neurotransmitters was obtained by the SET system analysis software in the whole-brain and 12 brain regions，including the left frontal area （F3），right frontal area （F4），left central area （C3），right central area （C4），left parietal area （P3），right parietal area （P4），left occipital area （O1），right occipital area （O2），left anterior temporal area （F7），right anterior temporal area （F8），left posterior temporal area （T5），and right posterior temporal area （T6） . The expression information of each neurotransmitter was compared with the normal model，and it was found that single neurotransmitter was in one of three states：increased，decreased，or normal. The simultaneous presentation of six neurotransmitters in the brain space is referred to as the"expression pattern of multiple neurotransmitters". 2. A MySQL database was established to analyze the actual expression patterns of different neurotransmitters in the whole-brain of individuals with depression . 3.Factor Analysis was used to further analyze the characteristic rules of combination with 78 variables of neurotransmitters in whole-brain and 12 multiple brain regions in depression.Results1 As for single neurotransmitter the whole-brain and different brain regions of total depression populations all showed one of three expression states （increased / decreased /normal），among with the majority being normal state. In the whole-brain：5-HT decreased by 6% and increased by 25%；ACh decreased by 31% and increased by 17%；DA decreased by 36% and increased by 9%；INH decreased by 15% and increased by 31%；EXC decreased by 32% and increased by 14%. NE decreased by 22% and increased by 22%.2. There is a significant antagonistic relationship in the whole-brain and different brain regions in each of ‘Antagonizing Pairs of Neurotransmitters’（EXC/INH；DA/5-HT；ACh/NE），between EXC and INH are high negatively correlated （P<0.01），with |r| values ranging from 0.69 to 0.76；between DA and 5-HT showed a high negative correlation （P<0.01），with |r| values ranging from 0.83 to 0.90；between ACh and NE showed a moderate negative correlation （P<0.01），with |r| values ranging from 0.56 to 0.66.Meanwhile in the whole-brain and different brain regions ，there is a low negative correlation （P<0.01） between non antagonistic pairs such as between DA and NE with |r| values ranging from 0.38 to 0.46；between DA and EXC showed a low positive correlation （P<0.01） with |r| values ranging from 0.38 to 0.47；between NE and EXC showed a moderate negative correlation （P<0.01） with |r| values ranging from 0.56 to 0.61. 3. The six neurotransmitters in the 1028 patients with depression were classified into a total of 170 types of actual expression patterns in the whole-brain by database processing and analysis. The top 30 types of expression patterns were reported in this paper，with a cumulative rate of 60.60%，such patterns as ①INH+/5-HT-/ACh+/DA+/NE-/EXC-，accounting for 9.05%；②INH+/5-HT-/ACh↓/DA+/NE-/EXC-，accounting for 4.57%；③INH+/5-HT-/ACh+/DA+/NE↓/EXC-，accounting for 3.31%，etc.In other words，the proportion of cases of depression with normal levels of all six neurotransmitters was 9.05%，and with including at least one neurotransmitter abnormality was 91.95%. 4. In this study，22 common factors were extracted from 78 variables in the whole-brain and different brain regions by the factor analysis，the absolute values of the factor loadings ranged from 0.32 to 0.86，and the eigenvalues （F）ranged from 1.03 to 13.43，with a cumulative contribution rate of 76.82%.List characteristic pattern such as：①AChP3↓/AChW↓/AChC3↓/AChF3↓/AChO1↓/AChT5↓/AChF7↓/NEP3↑/NEW↑/NEC3↑/NEF3↑/NEO1↑/NET5↑/NEF7↑，F=13.43；②5-HTO2↑/DAO2↓/5-HTP4↑/DAP4↓/DAP4↓/5-HTW↑/DAW↓/5-HTC4↑/DAC4↓，F=5.94；③EXCF4↓/DAF4↓/NEF4↑/INHF4↑/5-HTF4↑/AChF4↓，F=5.33，etc.ConclusionFirstly，the actual 170 types of expression patterns of 6 neurotransmitters in the whole-brain from 1028 depression populations was indicating that depression is a heterogeneous disease with individualized characteristics. Secondly，22 kinds of characteristic expression patterns were extracted from the whole-brain and 12 brain regions，which verified the pathogenesis hypothesis of " Encephalo Multi-Neurotransmitter Oscillation Imbalance in Depression". In summary，this study provides new guidance for the etiology，diagnosis and treatment of depression and also establishes methodological foundation for the individualized treatment effectiveness evaluation of depression in Traditional Chinese Medicine based on the objective biological markers .关键词：depression;Search of Encephalo-Telex（SET）;neurotransmitters;antagonizing pairs of neurotransmitters;heterogeneity;methodology4|0|0更新时间：2025-04-17
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Randomized Controlled Trail of Ganluqingwen Formula for Treatment of Community-acquired Pneumonia AI导读
“最新研究发现，甘露清瘟方联合西药治疗社区获得性肺炎效果显著，能降低中医证候积分，调节炎症与免疫反应。”摘要：ObjectiveTo explore the regulatory effect of Ganluqingwen Formula on inflammation and immunity by observing the clinical efficacy of Ganluqingwen Formula in the treatment of community-acquired pneumonia （CAP）， so as to provide a clinical basis for the treatment of CAP by traditional Chinese medicine （TCM）.MethodsA randomized controlled trial was conducted by selecting patients who were diagnosed with CAP and identified as wind-heat attacking lungs in Xinjiang Uygur Autonomous Region Hospital of TCM from January 2024 to May 2024 and assigning the patients to a control group （treated by western medicine treatment） or an experimental group （treated by Ganluqingwen Formula combined with western medicine）. The data of the enrolled patients before treatment， for three-day treatment， for seven-day treatment， and for 14-day treatment were collected， including basic information， medical history， pneumonia severity index （PSI） classification， and distribution and difference of laboratory and imaging information indexes. The peripheral blood specimens were collected from the patients. and the changes of inflammatory factors in peripheral blood were detected by using enzyme-linked immunosorbent assay （ELISA） reagent kits and flow-type multifactor microarrays to evaluate the clinical safety and efficacy of Ganluqingwen Formula in CAP.ResultsCompared with those in the groups before treatment， the total scores of TCM syndromes significantly decreased in both groups （P<0.05）. Compared with those in the control group after treatment， the total scores of TCM syndromes decreased more significantly in the experimental group （P<0.05）. Compared with the control group after treatment， the experimental group displayed a significantly reduced number of days of fever in patients （P<0.05）. Compared with those in the groups before treatment， the leukocyte， neutrophil counts， C-reactive protein （CRP）， procalcitonin （PCT）， interleukin （IL）-6， alanine aminotransferase （ALT）， aspartate aminotransferase （AST）， creatinine （Cr）， creatine kinase （CK）， and creatine kinase isoenzymes （CK-MB） in both groups decreased （P<0.05） after treatment. Compared with that in the control group after treatment， the decrease of leukocyte， neutrophil counts， CRP， PCT， IL-6， ALT， AST， Cr， CK， and CK-MB was more pronounced in the experimental group （P<0.05）. Compared with those in the group before treatment， the partial pressure of carbon dioxide increased in the experimental group for 3 d of treatment （P<0.05）， and the standard alkali residual， actual alkali residual， standard bicarbonate concentration， and actual bicarbonate concentration increased in the experimental group for 7 d of treatment （P<0.05）. Compared with that in the group before treatment， D-dimer decreased in the control group for 7 d of treatment （P<0.05）. D-dimer and activated partial thromboplastin time （APTT） decreased in the experimental group for 3 d of treatment （P<0.05）， and D-dimer， fibrinogen （FIB）， and APTI significantly decreased in the group for 7 d of treatment （P<0.05）. Compared with the group for 3 d of treatment， the experimental group for 7 d of treatment showed decreased FIB （P<0.05）. Compared with those in the groups before treatment， the levels of inflammatory factors IL-4， IL-10， and IL-13 were elevated in the peripheral blood of the two groups after treatment， and the levels of B lymphocyte chemoattractant （BLC）， interferon gamma-induced protein 10 （IP-10）， tumor necrosis factor-alpha （TNF-α）， interferon-gamma （IFN-γ）， monocyte chemoattractant protein-1 （MCP-1）， CRP， IL-2， IL-6， IL-8， IL-17， IL-22， and IL-23P19 were significantly reduced （P<0.01）. Compared with the control group after treatment， the experimental group exhibited more significant improvement in indexes above （P<0.01）.ConclusionThe group treated by Ganluqingwen Formula combined with western medicine shows more significant effects on reducing total scores of TCM syndromes， lowering the ability of leukocyte and neutrophil counts， decreasing BLC， IP-10， TNF-α， IFN-γ， MCP-1， CRP， IL-2， IL-6， IL-8， IL-17， IL-22， and IL-23P19 in the peripheral blood of the patients， and elevating levels of IL-4， IL-10， and IL-13 than the group treated by western drugs alone.关键词：Ganluqingwen formula;community-acquired pneumonia;randomized controlled study;Ganlu Xiaodu Decoction;Erchen decoction;wind-heat attacking lung4|0|0更新时间：2025-04-17 - 摘要：ObjectiveTo investigate the role of silent information regulatory protein （SIRT6）/hypoxia-inducible factor-1α （HIF-1α） pathway in regulating the reprogramming of glucose metabolism in ulcerative colitis （UC） and the mechanism of intervention of Shaoyaotang （芍药汤）.MethodsForty-eight c57bL/6 mice were randomly divided into a blank group， a model group， a Mesalazine group （0.42 g·kg-1）， a Shaoyaotang group （31.08 g·kg-1）， an inhibitor group （OSS-128167， 50 mg·kg-1）， and an inhibitor + Shaoyaotang group （50 mg·kg-1 OSS-128167 + 31.08 g·kg-1 Shaoyaotang）. A UC model was established by the administration of 2.5% dextran sulfate sodium （DSS） solution for mice in other groups for 7 d， except for the blank group. The mice in each group were treated with saline， Mesalazine， Shaoyaotang， inhibitor， and inhibitor + Shaoyaotang， respectively， for 7 d. The mice were necropsied 24 h after the last administration of the drug. The blood was collected from the orbital region， and colon tissue was taken. Hematoxylin-eosin （HE） staining was used to observe the pathological changes in colon tissue. Enzyme-linked immunosorbent assay （ELISA） was employed to detect serum interleukin （IL）-10， IL-17， and IL-6 levels. A biochemical method was used to detect glucose and lactate dehydrogenase A （LDHA） levels. Immunohistochemistry （IHC） was employed to detect IL-22 and transforming growth factor-β1 （TGF-β1） levels in colon tissue， and Western blot and real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） were used to detect relative protein and mRNA expressions of SIRT6， HIF-1α， and LDHA.ResultsCompared with those of the blank group， disease activity index （DAI） scores of mice in the model group and inhibitor group were significantly increased （P<0.01）. The length of colon tissue was significantly shortened， and colon tissue was congested and eroded. The pathohistological scores were significantly increased （P<0.01）. The levels of serum inflammatory factors IL-17 and IL-6 were significantly elevated， and the levels of IL-10 were significantly decreased （P<0.01）. The protein expressions of IL-22 and TGF-β1 were significantly reduced in colon tissue （P<0.01）. The relative protein and mRNA expressions of SIRT6 were significantly decreased （P<0.01）， and the relative protein and mRNA expressions of HIF-1α and LDHA and the contents of glucose and lactate were significantly elevated （P<0.01）. The level of inflammation in the colon of the mice in the inhibitor group was more severe than that in the model group （P<0.01）. Compared with the model group， the Mesalazine group， the Shaoyaotang group， and the inhibitor + Shaoyaotang group showed reduced colonic injury， significant decrease in serum IL-17 and IL-6， significant increase in IL-10 （P<0.01）， significant increase in the protein expressions of IL-22 and TGF-β1 in colon tissue （P<0.01）， significant increase in the protein expressions of SIRT6 and the relative mRNA expressions （P<0.01）， and significant reduction in the protein expressions of HIF-1α and LDHA， the relative mRNA expressions， and the contents of glucose and lactate （P<0.01）. Compared with those in the Shaoyaotang group， the serum IL-17 and IL-6 were significantly increased， and IL-10 was significantly decreased in the inhibitor + Shaoyaotang group （P<0.01）. The protein expressions of IL-22 and TGF-β1 in colon tissue were significantly decreased （P<0.01）. The expressions of SIRT6 protein and the relative mRNA expressions were significantly decreased （P<0.01）. The protein expressions of HIF-1α and LDHA， the relative mRNA expressions， and the contents of glucose and lactate were significantly elevated （P<0.01）. However， the difference between the Shaoyaotang group and the Mesalazine group was not significant.ConclusionShaoyaotang can effectively treat DSS-induced mice with UC through the SIRT6/HIF-1α pathway， and its mechanism of action may be related to the regulation of the SIRT6/HIF-1α pathway and glucose metabolism reprogramming and the inhibition of glycolysis.关键词：Shaoyaotang;ulcerative colitis;silent information regulatory protein 6（SIRT6）;hypoxia-inducible factor-1α（HIF-1α）;glycolysis2|0|0更新时间：2025-04-17
- “最新研究发现，麻黄、细辛、附子不同配伍能有效减轻过敏性鼻炎小鼠炎症，降低IgE、IL-5分泌，其中麻黄与附子配伍在抑制ILC2s表达方面最具优势。”摘要：ObjectiveTo explore the effects of compatibility of Ephedrae Herba，Asari Radix et Rhizoma and Fuzi on the expression of innate lymphoid cells 2（ILC2s）-related factors in the lung of allergic rhinitis（AR）mice.MethodsAccording to the random number table method，Fifty-four C57BL/6J mice were randomly divided into blank group，model group，Mahuang Fuzi Xixintang group，Asari Radix et Rhizoma Fuzi group，Ephedrae Herba Asari Radix et Rhizoma group，Ephedrae Herba Fuzi group，Ephedrae Herba group，Fuzi group and Asari Radix et Rhizoma group with 6 mice in each group. Except the blank group，the other groups were induced by intraperitoneal injection of ovalbumin（OVA）and intranasal allergic rhinitis. After the model was made，the mice in the blank group and the model group were given 0.2 ml/d normal saline by gavage，while those in the Mahuang Fuzi Xixintang group（2.31 g/kg），Asari Radix et Rhizoma Fuzi group（1.54 g/kg），Ephedrae Herba Asari Radix et Rhizoma group（1.16 g/kg），Ephedrae Herba Fuzi group（1.93 g/kg），Ephedrae Herba group（0.77 g/kg），Fuzi group（1.16 g/kg），and Asari Radix et Rhizoma group（0.39 g/kg）were given corresponding drugs by gavage respectively，14 consecutive days. The survival status of mice in each group was observed and the levels of serum immunoglobulins E（IgE）were measured by enzyme-linked immunosorbent assay（ELISA），the pathological changes of nasal and lung tissues were observed by hematoxylin-eosin（HE），the expression of ILC2s in lung tissue of mice was detected by immunofluorescence（IF），the mRNA expressions of GATA binding protein 3（GATA3），retinoic acid receptor-related orphan receptor-α（RORα）and inhibitor of DNA binding 2（ID2）in the lung tissue of mice were detected by reverse transcription polymerase chain reaction（REAL-TIME PCR），the levels of IgE，interleukin（IL）-4，IL-5 and IL-13 in serum were detected by ELISA.ResultsCompared with the blank group，the survival state of the model mice was poor，the serum IgE levels in the model group was significantly increased after nasal challenge（P<0.01），and a large amount of neutrophil infiltration was observed in the mucosa of the posterior turbinate with obvious nasal mucosal bleeding and purulent secretion，it was found that the epithelium was shed，the bronchial wall was thickened，the intravascular hyperemia and edema were obvious，a large number of inflammatory cells were diffuse and infiltrated，the alveolar structure was seriously damaged，and the local lung consolidation was observed，the expression of ILC2s in the lung tissue was significantly increased（P<0.01），the expression of GATA3 mRNA and RORα mRNA was increased，while the expression of ID2 mRNA was decreased（P<0.05，P<0.01），and the levels of serum IgE and IL-4，IL-5，IL-13 were increased（P<0.05，P<0.01）. Compared with the model group，the survival state and the pathological changes in the nasal and lung tissues were significantly alleviated，inflammatory cell infiltration was significantly reduced，a small amount of nasal mucosa bleeding，trachea wall thinning，no hyperemia，edema and nasal secretions were observed in the Mahuang Fuzi Xixintang and its separated prescription. The expression of ILC2s in lung tissue was significantly decreased（P<0.01）. The expression levels of GATA3mRNA was decreased（P<0.05），especially in Fuzi group（P<0.01），Only the expression levels of RORα mRNA in Ephedrae Herba Fuzi group and Ephedrae Herba group decreased（P<0.05）. The levels of serum IgE were decreased（P<0.05）and IL-5 were significantly decreased（P<0.01）. IL-4 were significantly decreased in all groups except Aconiti Lateralis Radix Praeparata group（P<0.01），the levels of IL-13 in Ephedrae Herba Asari Radix et Rhizoma Aconiti Lateralis Radix Praeparata Decoction group was decreased（P<0.05），and the levels of IL-13 in Ephedrae Herba Aconiti Lateralis Radix Praeparata group，Ephedrae Herba group，Aconiti Lateralis Radix Praeparata group，Asari Radix et Rhizoma group were significantly decreased（P<0.01）.ConclusionDifferent compatibility of Ephedrae Herba，Asari Radix et Rhizoma and Aconiti Lateralis Radix Praeparata could reduce the inflammation of OVA-induced allergic rhinitis mice，and had more advantages in reducing the secretion of IgE and IL-5. The compatibility of Ephedrae Herba and Aconiti Lateralis Radix Praeparata has the most advantage in reducing the expression of GATA3 mRNA and RORα mRNA to inhibit the expression of ILC2s and thus exert the anti-allergic effect，while the other compatibility has the most extensive advantage in inhibiting the expression of GATA3 mRNA.关键词：Ephedrae Herba;Asari Radix et Rhizoma;Aconiti Lateralis Radix Praeparata;compatibility;allergic rhinitis;innate lymphoid cells 2（ILC2s）;mice4|0|0更新时间：2025-04-17
- “在特发性肺纤维化治疗领域，研究显示中药复方参龙煎剂能减轻症状、提升运动耐量和生活质量，对改善肺功能具有潜在优势。”摘要：ObjectiveTo assess the therapeutic effectiveness and safety of the traditional Chinese medicine compound Shenlong decoction in addressing the symptoms of pulmonary deficiency and stasis in patients with idiopathic pulmonary fibrosis （IPF）.MethodsSixty eligible patients with lung deficiency and collateral stasis syndrome of IPF were randomly assigned to the observation （30 patients） and control groups （30 patients）. All patients underwent standard Western medical therapy. Additionally，the observation group received Shenlong decoction granules，while the control group received a placebo. Both treatments were packaged in four doses of 10.5 g each，taken twice daily for three months. The indexes of the patients during the treatment cycle were observed，and the main indexes include traditional Chinese medicine （TCM） syndrome scores and 6 min walk test （6MWT）. The secondary indexes include pulmonary function test ［actual value/expected value of total lung volume （TLC%），actual value/expected value of vital capacity（FVC%），actual/predicted diffusing capacity of the lung for carbon monoxide（DLCO%），actual/predicted forced expiratory volume in one second （FEV1%），and FEV1/ forced vital capacity （FVC）］，blood gas analysis ［arterial blood diathesis partial pressure of oxygen （PaO2），partial pressure of carbon dioxide （PaCO2），and arterial oxygen saturation （SaO2）］，serum inflammatory factors ［transforming growth factor-β1 （TGF-β1），interleukin-4 （IL-4），interleukin-13 （IL-13），interleukin-12 （IL-12），and gamma-interferon （IFN-γ）］，and quality of survival evaluation ［St George's Respiratory Questionnaire （SGRQ） score］. The patients' clinical manifestations were determined at the end of the treatment， and the occurrence of adverse events was recorded.ResultsA total of 53 patients completed the study，comprising 27 in the control group and 26 in the observation group. Upon completion of the treatment period，the control group achieved a total effective rate of 33.33% （9/27），whereas the observation group demonstrated a total effective rate of 53.85% （14/26），which was statistically superior to the control group （χ2=4.034，P<0.05）. After the treatment，the TCM syndrome scores，6MWT，DLCO%，FEV1%，PaO2，PaCO2，TGF-β1，IL-4，IL-13，IL-12，and IFN-γ in the two groups were all significantly improved （P<0.05，P<0.01）. Compared with those in the control group after treatment at the same period，the TCM syndrome scores，6MWT，PaO2，and PaCO2 were significantly improved in the observation group after 60 days and 90 days of medication （P<0.01）. Three months after the end of medication，the SGRQ score in the observation group showed significant improvement when compared to that in the control group （P<0.05），and no severe adverse events were reported during the follow-up period.ConclusionCompound Shenlong decoction can alleviate clinical symptoms such as shortness of breath and wheezing in patients with lung deficiency and collateral stasis syndrome of IPF，enhance exercise tolerance，improve the quality of life，and have certain potential advantages in improving pulmonary function.关键词：Shenlong decoction;idiopathic pulmonary fibrosis;lung deficiency and collateral stasis syndrome;randomized controlled trial;clinical efficacy3|0|0更新时间：2025-04-17
- “醒脾胶囊改善功能性消化不良效果显著，可能通过抑制PI3K/Akt信号通路减轻十二指肠低度炎症，E-橙花叔醇等为其关键活性成分。”摘要：ObjectiveTo investigate the ameliorative effect and potential mechanism of Xingpi Capsule on functional dyspepsia（FD）.MethodsSixty male SD neonatal rats of SPF grade（7 days old） were randomly divided into the normal group（n=12） and the modeling group（n=48）， and the FD model was prepared by iodoacetamide gavage in the modeling group. After the model was successfully prepared， the rats in the modeling group were randomly divided into the model group， the low-dose group（0.135 g·kg-1）， the high-dose group（0.54 g·kg-1） and the domperidone group（3 mg·kg-1）， with 12 rats in each group. The rats in the normal and model groups were gavaged with distilled water， and the rest of the groups were gavaged with the corresponding medicinal solution once a day for 7 d. The general survival condition of the rats was observed， and the water intake and food intake of the rats were measured； the gastric emptying rate and the small intestinal propulsion rate were measured at the end of the treatment； the pathological damage of the rat duodenum was examined by hematoxylin-eosin staining； and the colonic tight junction protein， Occludin， was detected by immunofluorescence method in the rats， ZO-1 expression； The differentially expressed genes in the duodenal tissues of the model group and the normal group， and the Xingpi Capsules dosage group and the model group were detected by transcriptomics sequencing technology after the final administration， and Gene Ontology（GO） and Kyoto Encyclopedia of Genes and Genomes（KEGG） enrichment analyses were carried out； the transcriptomic results were validated and screened for molecular docking of active ingredients with key targets by protein immunoblotting（Western blot）， immunofluorescence， and real-time fluorescence quantitative polymerase chain reaction（ Western blot， immunofluorescence and real-time fluorescence quantitative polymerase chain reaction（real-time PCR） were used to validate the transcriptomics results and to screen the active ingredients of Xingpi Capsules for molecular docking with the key targets.ResultsCompared with the normal group， the general survival condition of rats in the model group was poorer， and the water intake， food intake， gastric emptying rate and small intestinal propulsion rate were all significantly reduced（P<0.05）； inflammatory infiltration was seen in duodenal pathology； and the fluorescence intensities of Occludin and ZO-1 in colon were significantly reduced（P<0.01）； compared with the model group， the general survival condition of rats in the high dose group of Xingpi Capsules improved significantly， and the general survival condition of rats in the high dose group of Xingpi Capsules was significantly improved. Water intake， food intake， gastric emptying rate and small intestinal propulsion rate were all significantly higher（P<0.05）； duodenal pathology showed a decrease in inflammatory infiltration； colonic Occludin and ZO-1 fluorescence intensity was significantly higher（P<0.01）； transcriptomics results showed that Xingli Capsules may regulate phosphatidylinositol 3-kinase（PI3K）/protein kinase B（A3K） /protein kinase B（A3K） through key mRNAs， such as Slc5a1， Abhd6 and other mRNAs. The validation results showed that the phosphorylation levels of PI3K and Akt were significantly higher in the model group compared with the normal group（P<0.05）， the protein expression level of interleukin 1β（ IL-1β ） was significantly higher in the model group compared with the normal group（P<0.01）， and the fluorescence intensities of interleukin 6（IL-6） and IL-1β were significantly higher in the model group（P<0.01）； The mRNA levels of key genes such as Slc5a1， Abhd6， Mgam， Atp1a1， Slc7a8， Cdr2， Chrm3， and Slc5a9 were significantly increased（P<0.01）. Compared with the model group， the phosphorylation levels of PI3K and Akt in rats in the high-dose group of Xingpi Capsules were significantly reduced（P<0.05）， and the protein expression level of IL-1β were significantly reduced（P<0.01）； the fluorescence intensity of IL-6 and IL-1β was significantly reduced（P<0.01）； the fluorescence intensity of key genes such as Slc5a1， Abhd6， Mgam， Atp1a1， Slc7a8， Cdr2， Chrm3， and Slc5a9 and other key genes had significantly lower mRNA levels（P<0.05）. Weighted gene co-expression network analysis and molecular docking results showed that the components of Xingpi Capsules， E-nerolidol and Z-nerolidol， were well bound to ABDH6 protein， and linarionoside A， valerosidatum and senkirkine were well bound to SLC5A1 protein.ConclusionXingpi capsule can effectively improve the general survival and gastrointestinal motility of FD rats， which may improve FD through multi-targets， and its specific mechanism may be related to the inhibition of PI3K/Akt signaling pathway to reduce the low-grade inflammation of duodenum.关键词：Xingpi capsules;functional dyspepsia;transcriptomics;low-grade inflammation of duodenum;phosphatidylinositol 3-kinase/protein kinase B（PI3K/Akt） signaling pathway;molecular docking3|0|0更新时间：2025-04-17
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