最新刊期
- “最新研究发现，PI3K/Akt信号通路在哮喘病理进展中发挥关键作用，中医药干预该通路有望为哮喘治疗提供新思路。”摘要：Asthma is a chronic inflammatory respiratory disease involving multiple cells and cellular components， characterized by recurrent episodes of wheezing， shortness of breath， chest tightness， and coughing， significantly impacting patients' quality of life. The phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt） signaling pathway serves as a crucial hub in intracellular signaling， regulating cell growth， proliferation， survival， metabolism， and various pathophysiological processes. Its role in the pathological progression of asthma is profound， evident in promoting airway inflammation， mediating epithelial mesenchymal transformation， accelerating airway remodeling， regulating cell autophagy， inducing mucus hypersecretion， and influencing immune response balance. This study analyzes potential molecular targets of the PI3K/Akt pathway， identifying activators such as cysteine protease inhibitor Cystatin SN （CST1）， Found in Inflammatory Zones-1 （FIZZ1）， and Free Fatty Acid Receptor 1 （FFAR1）. Additionally， inhibitors such as human β-defensin 3 （hBD-3）， desintegrins， and metalloproteinase-33 （ADAM33） are examined as markers of airway inflammation and potential pathway inhibitors. Notably， interleukin-27 （IL-27） is also discussed. The paper initially unveils the potential molecular mechanisms of Traditional Chinese Medicine （TCM） in asthma intervention. The authors systematically summarize the efficacy and specific mechanisms of TCM monomers， combinations， and external treatments in regulating the PI3K/Akt signaling pathway through literature review and analysis. This aims to establish a robust foundation for the widespread application and advanced development of TCM in asthma treatment， offering innovative insights for clinical research and drug development in asthma management.关键词：traditional Chinese medicine;asthma;phosphatidylinositol 3-kinase（PI3K）/protein kinase B（Akt） signaling pathway;airway inflammation;airway remodeling;potential targets;research progress1|0|0更新时间：2025-07-11
- “最新研究揭示鸡骨草及毛鸡骨草的历史沿革和药用价值，为传统名方开发提供科学依据。”摘要：This article systematically analyzes the historical evolution of the name， origin， academic name， medicinal parts， origin， harvesting， processing and processing of Abri Herba and Abri Mollis Herba by referring to the herbal medicine， medical books， prescription books and other documents of the past dynasties， combined with the relevant modern research materials. Carding and textual research provide a basis for the development of classic prescriptions containing this type of medicinal materials. According to the verification， Abri Herba was first recorded in Lingnan Caiyaolu， with other names such as HuangTouCao and XiYeLongLinCao. It originates from the dried whole plant of Abrus cantoniensis， a Fabaceae plant， which can be used medicinally except for its fruits. Currently， this species is mainly distributed in GuangDong and GuangXi， and also found in HuNan and Thailand； it can be harvested throughout the year， with spring and autumn being the main seasons. The roots， stems， and leaves can be used for medicinal purposes， but the pods are toxic and must be removed. After harvesting， impurities and pods are removed， and it is dried and processed for medicinal use. Abri Herba has a sweet and slightly bitter taste， is cool in nature， and is associated with the liver and stomach meridians； it is used for clearing heat and dampness， dispersing blood stasis， and relieving pain； and is mainly used to treat jaundice-type hepatitis， stomach pain， rheumatic bone pain， contusion and ecchymosis pain， and mastitis.Abri Mollis Herba was first recorded in the 1982 edition of Zhongyaozhi， used in conjunction with A. cantoniensis. It was listed in Xinhua Bencao Gangyao in 1988. Other monographs also list it as a single herb， with its aliases and efficacy， while some other herbs use it as a local habitual product or confused product of Abri Herba. with other names such as DaYeJiGuCao， QingTingTeng， and MaoXangShi. It comes from the dried whole herb of Abrus molliswithout pods， mainly produced in GuangXi and GuangDong， and occasionally found in Hong Kong， HaiNan， and FuJian. The collection and processing are similar to Abri Herba； after harvesting， impurities and pods are removed， and it is dried and cut for medicinal use. Abri Mollis Herba has a sweet and light taste， is cool in nature， and is associated with the liver and stomach meridians， with effects of clearing heat and detoxifying， and promoting dampness； it is mainly used to treat infectious hepatitis， mastitis， furuncles， burns and scalds， and pediatric malnutrition. Based on the research， A. mollis was first recorded to be used as a medicine in the same base as A. cantoniensis， and as plants of the same genus， have very similar morphological characteristics， and their medicinal parts， collection and processing， properties and flavors， and meridian affiliations are consistent. And A. mollis in the folk have as Abri Herba use， which has been used for a long time and is now dominated by the cultivation of A. mollis. so it is recommended that the Chinese Pharmacopoeia will be A. mollis can be with the inclusion of Abri Herba into the same medicinal herbs base material， and it is recommended that in classical prescriptions refered to Jiguccao can use A. cantoniensis and A. mollis as the sources of the herbs to be used together in the medicine. Refered to MaoJiguccao can use A. mollis as the sources of the herbs to be used respectively. Prepare according to the preparation requirements specified in the original prescription， and if there are no requirements， use the raw product for medicine.关键词：famous classical formulas;Abri Herba;Abri Mollis Herba;origin;scientific name;medicinal parts;processing;herbal textual research2|0|0更新时间：2025-07-11
- “最新研究发现，现代工艺与古法工艺制备的百合地黄汤质量标志物含量相当，药效作用一致，为百合地黄汤新药开发提供科学依据。”摘要：ObjectiveBased on analysis and chronic unpredictable mild stress（CUMS）， the depression model was established to evaluate the quality components and pharmacological effects of Baihe Dihuangtang prepared by different techniques.MethodsHPLC was used to establish the characteristic profiles of Baihe Dihuangtang， The content of Q-Marker and the yield of Baihe Dihuangtang prepared by ancient and modern techniques were measured separately；Seventy C57BL/6J mice were randomized into normal， model， fluoxetine（3 mg·kg-1）， low-and high-dose（6. 5， 26 g·kg-1， respectively）Baihe Dihuangtang by ancient techniques， and low-and high-dose（6. 5， 26 g·kg-1， respectively）Baihe Dihuangtang by modern techniques groups， with 10 mice in each group. Other groups except the normal group was induced by CUMS method in the modeling group to induce depression. After 28 days of stimulation， administration groups were given relevant medicine intragastrically， once a day， for 21 consecutive days. The behavioral indicators such as changes in body mass， tail suspension test， and open field test were detected in each group；Enzyme-linked immunosorbent assay（ELISA）was employed to determine the content of tumor necrosis factorα（TNF-α）， interleukin1β（IL-1β）and interleukin6（IL-6）.ResultsThe similarity of Baihe Dihuangtang characteristic profiles was>0. 999， and 23 common peaks were calibrated， nine chromatographic peaks were identified. The Q-Marker content in Baihe Dihuangtang prepared by ancient and modern techniques is equivalent， with average paste yields of 17. 20%and 17. 07%， respectively. Compared with normal group， the model group showed increased immobility time in the tail suspension test（P<0. 01）， and reduced residence time in the central area of the open field and the total movement distance（P<0. 01）， In addition， the modeling elevated the level IL-1β， IL-6， and TNF-α in the serum（P<0. 01）. Compared with the model group， the behavioral indicators of mice in the Baihe Dihuangtang treatment group were significantly improved in terms of tail suspension time and open field exercise（P<0. 05， P<0. 01）， and the levels of IL-1β， IL-6， and TNF-α in serum were significantly reduced（P<0. 05， P<0. 01）. Both Baihe Dihuangtang treatments had antidepressant effects， and there was no significant difference in improving depressive symptoms between two treatments.ConclusionThe Q-Marker of Baihe Dihuangtang prepared by modern and ancient methods are equivalent in content， and the pharmacological effects are consistent. This indicates that dried lilies can replace fresh lilies in the preparation of Baihe Dihuangtang， providing a scientific basis for the development of new drugs for Baihe Dihuangtang and a reference for its rational application and clinical use.关键词：Baihe Dihuangtang;quality markers;characteristic mapping;pharmacological research;chronic unpredictable mild stress（CUMS）;antidepressant4|0|0更新时间：2025-07-11
- “紫苏梗质量差异研究取得进展，专家构建了熵权-TOPSIS模型，为紫苏梗质量评价提供解决方案。”摘要：ObjectiveBased on the content of volatile and nonvolatile components in Perillae Caulis， this article explored differential components of Perillae Cauliss with different characters and origins， and screened the premium Perillae Cauliss.MethodsThis study collected 32 batches of Perillae Cauliss from 12 producing areas recording their diameters， epidermis colors and producing areas. The contents of total flavonoids， total phenols， volatile oils， 5 active components and 84 volatile components in 32 batches of Perillae Cauliss were quantitatively or semi-quantitatively determined using microplate reader， UPLC-PDA and GC-MS. Then the component content difference was analyzed using PCA and nonparametric test. Based on the weight of indices from PCA model， TOPSIS model was constructed to evaluate the quality of Perillae Cauliss with different characters and origins.ResultsThere were significant differences in the composition of Perillae Cauliss with different diameters， epidermis colors and producing areas， with 9 differential components screened， including 6 key indices and 3 volatile compounds， namely total flavonoids， total phenols， caffeic acid， scutellarin， rosmarinic acid， luteolin； caryophyllene oxide， （-）-humulene epoxide Ⅱ， 14-hydroxycaryophyllene. The content of 6 index components was higher in Perillae Cauliss with small diameter， purple brown skin and southern origins while the content of 3 volatile components was higher in Perillae Cauliss with large diameter， dark brown skin and northern origins. A significant difference was shown in the model scores of different diameters， skin colors and origins （P < 0.01）. Perillae Cauliss with small diameter， purple-brown skin， from southern area especially Guangdong， had a high score.ConclusionInfluenced by the diameter， skin color and producing area， the chemical composition and content of Perillae Cauliss varied. Perillae Cauliss showing small diameter， owing purple-brown skin， and originating from Guangdong Province were of higher-quality due to their higher content of 8 key indices.关键词：appearance;origin;entropy weight-technique for order preference by similarity to an ideal solution（TOPSIS）;chemometrics;quality difference;Perillae Caulis6|0|0更新时间：2025-07-11
- “最新研究发现，鼻腔给药联合新型递药系统，能有效提高中药成分脑内浓度，为中枢神经系统疾病治疗提供新策略。”摘要：Central nervous system （CNS） diseases are significantly hindered by their complex pathological mechanisms and the presence of the blood-brain barrier （BBB）， which limits the effectiveness of drug treatments. Traditional drug administration methods， such as oral administration， intravenous injection， and transdermal delivery， despite having certain advantages， all face the challenge that drugs struggle to effectively cross the BBB. Therefore， identifying drug delivery methods that can efficiently traverse the BBB is crucial. Nasal drug delivery， as a non-invasive route， offers the potential for targeted CNS delivery via three pathways： olfactory neurons， trigeminal neurons， and the bloodstream. This approach holds considerable promise in the treatment of CNS diseases. Compared to chemical drugs， traditional Chinese medicine （TCM） features multiple components， multiple targets， and fewer adverse effects， providing unique advantages in the treatment of CNS diseases. A substantial body of research has further confirmed that nasal delivery combined with novel drug delivery systems such as lipid nanoparticles， nanoparticles， nanoemulsions， and composite in situ gels can effectively load the active components of TCM， significantly enhancing drug concentration in the brain and offering new strategies for the treatment of CNS diseases. This article systematically reviews the current state of drug delivery for CNS diseases， delves into the characteristics of nasal drug delivery， and focuses on summarizing and analyzing the research progress of passive targeting， active targeting， and "guided" drug delivery strategies for the nasal-to-brain transport of TCM active components. The aim is to provide references and insights for the development of drugs for CNS diseases and the application of TCM in nasal-to-brain delivery.关键词：traditional Chinese medicine;nasal delivery;central nervous system diseases;blood-brain barrier;drug delivery system;guide medicine upward;research progress4|0|0更新时间：2025-07-11
- 摘要：“Liver being substantial Yin and functional Yang” maintain normal function of qi， blood and meridians. In clinical practice， it is often found that pan vascular lesions with atherosclerosis as the predominant pathologic change often co-occur with metabolic dysfunction-associated fatty liver disease（MAFLD）. MAFLD leads to increased risk and worse prognosis for many pan vascular diseases， including cardiovascular disease. Dysregulation of energy homeostasis disrupts the hepatic homeostasis of body use， and representative drugs to improve metabolism， such as metformin， sodium-glucose cotransporter 2 inhibitors， and glucagon-like peptide-1 agonists， not only have a clear cardiovascular benefit， potential improvement of MAFLD has also been demonstrated. The liver stores blood and the heart pumps blood， and liver diseases affect the heart， that's why the unsmoothness of vessels appears. So the treatment should from the standpoint of liver， restoring liver function， soothing the liver and nourish heart， activating blood and dredging meridian. It is of great significance to explore in depth the pathogenesis and treatment of pan vascular lesions caused by MAFLD， and to restore the energy homeostasis by adjusting the balance of liver yin and yang.关键词：liver being substantial Yin and functional Yang;metabolic dysfunction-associated fatty liver disease;panvasculopathy;energy metabolism;imbalance of Yin and Yang;atherosclerosis;traditional Chinese medicine pathogenesis0|0|0更新时间：2025-07-11
- “最新研究发现，生脉救心汤能改善气阴两虚、阳虚血瘀型慢性心力衰竭失代偿患者心功能与临床症状，其机制与调控HIF-1通路HIF-1α、VEGF-A和Caspase-3等靶点有关。”摘要：ObjectiveTo investigate the clinical efficacy and potential mechanism of Shengmai Jiuxin decoction in the treatment of acute decompensated heart failure （ADHF） with the traditional Chinese medicine （TCM） pattern of Qi and Yin deficiency， Yang deficiency， and blood stasis.MethodsA total of 68 patients diagnosed with ADHF of Qi and Yin deficiency， Yang deficiency， and blood stasis type were randomly assigned to an observation group （n=34） and a control group （n=34）. Both groups received conventional Western medical treatment， while the observation group was additionally administered Shengmai Jiuxin decoction. Parameters compared before and after treatment included： TCM syndrome score， TCM syndrome efficacy， New York Heart Association （NYHA） functional classification， left ventricular ejection fraction （LVEF）， N-terminal pro-B-type natriuretic peptide （NT-proBNP）， six-minute walk distance （6-MWD）， hypoxia-inducible factor-1 alpha （HIF-1α）， vascular endothelial growth factor A （VEGF-A）， Caspase-3， and the number of rehospitalizations for heart failure within one month after discharge.ResultsThere were no significant differences in sex， age， vital signs， or underlying diseases between the two groups. Compared with baseline， both groups exhibited significant reductions in TCM syndrome scores， NT-proBNP， and HIF-1α levels （P<0.01）， as well as significant increases in 6-MWD， LVEF， VEGF-A， and Caspase-3 levels （P<0.05， P<0.01）. After treatment， the observation group showed significantly greater reductions in TCM syndrome score， NT-proBNP， HIF-1α， and Caspase-3 levels compared with the control group （P<0.05） and significantly greater increases in 6-MWD， TCM syndrome efficacy， and VEGF-A levels （P<0.05）. No significant differences were observed between the groups in NYHA functional classification， LVEF， or the number of rehospitalizations for heart failure within one month after discharge. No drug-related adverse events were reported in either group during the treatment period.ConclusionShengmai Jiuxin decoction can improve cardiac function and clinical symptoms in patients with ADHF of Qi and Yin deficiency， Yang deficiency， and blood stasis type. Its mechanisms may be related to the regulation of the HIF-1 signaling pathway by modulating targets such as HIF-1α， VEGF-A， and Caspase-3.关键词：Shengmai Jiuxin decoction;acute decompensated heart failure;Qi and Yin deficiency， Yang deficiency， and blood stasis type;HIF-1 signaling pathway6|0|0更新时间：2025-07-10
- “据最新研究报道，麻杏蒌石汤方治疗慢性阻塞性肺疾病急性加重疗效显著，能降低炎症因子，改善免疫功能，调节PD-1/PD-L1信号通路。”摘要：ObjectiveTo evaluate the clinical efficacy of Maxing Loushi decoction in the treatment of acute exacerbation of chronic obstructive pulmonary disease （AECOPD） with phlegm turbidity obstructing lung syndrome， and to investigate its effects on inflammatory factors， immune function， and the programmed death-1（PD-1）/programmed death-ligand 1 （PD-L1） signaling pathway.MethodsA randomized controlled study was conducted， enrolling 90 hospitalized patients with AECOPD and phlegm turbidity obstructing lung syndrome in the Respiratory and Emergency Departments of Dongzhimen Hospital， Beijing University of Chinese Medicine， from April 2024 to December 2024. Patients were randomly assigned to a control group and an observation group using a random number table， with 45 patients in each group. The control group received conventional Western medical treatment， while the observation group received additional Maxing Loushi decoction for 14 days. Clinical efficacy， COPD Assessment Test （CAT） score， modified Medical Research Council Dyspnea Scale （mMRC）， 6-minute walk test （6MWT）， serum inflammatory factors， T lymphocyte subsets， and serum PD-1/PD-L1 levels were compared between the two groups before and after treatment.ResultsThe total clinical effective rate was 78.57% （33/42） in the control group and 95.35% （41/43） in the observation group， with the observation group showing significantly higher efficacy than that of the control group. The difference was statistically significant （χ2 = 5.136， P<0.05）. After treatment， both groups showed significant reductions in CAT and mMRC scores （P<0.05， P<0.01） and significant increases in 6MWT compared to baseline （P<0.01）. The observation group demonstrated significantly greater improvements than the control group in this regard. Levels of inflammatory markers including C-reactive protein （CRP）， procalcitonin （PCT）， interleukin-6 （IL-6）， tumor necrosis factor-α （TNF-α）， monocyte chemoattractant protein-1（MCP-1）， and macrophage inflammatory protein-1α （MIP-1α） were significantly reduced in both groups （P<0.05， P<0.01）， with greater reductions in the observation group （P<0.05， P<0.01）. CD8+ levels were significantly reduced （P<0.01）， while CD3+， CD4+， and CD4+/CD8+ levels were significantly increased in both groups after treatment （P<0.05， P<0.01）， with more significant improvements observed in the observation group （P<0.05， P<0.01）. Serum PD-1 levels were reduced （P<0.05， P<0.01）， and PD-L1 levels were increased significantly in both groups after treatment （P<0.05， P<0.01）， with more pronounced changes in the observation group （P<0.05）.ConclusionMaxing Loushi decoction demonstrates definite therapeutic efficacy as an adjunctive treatment for patients with AECOPD and phlegm turbidity obstructing lung syndrome. It contributes to reducing serum inflammatory factors， improving immune function， and regulating the PD-1/PD-L1 signaling pathway.关键词：acute exacerbation of chronic obstructive pulmonary disease;Maxing Loushi decoction;inflammatory factor;immune function;programmed death-1（PD-1）/programmed death-ligand 1（PD-L1） signaling pathway5|0|0更新时间：2025-07-10
- “据最新研究，中医药在慢性萎缩性胃炎“炎-癌”转化机制中显示出独特优势，为预防和治疗胃癌提供新思路。”摘要：“Inflammation-cancer” transformation of chronic atrophic gastritis （CAG） refers to the process in which the gastric mucosa， in the context of CAG， progresses through stages of precancerous lesions of gastric cancer （PLGC）， such as intestinal metaplasia and dysplasia， and eventually develops into gastric cancer （GC）. In China， the incidence and mortality rates of GC rank among the highest in the world， and the proportion of GC cases caused by gastric mucosal infection and inflammation has been increasing. Modern medical treatments for CAG and PLGC mainly rely on drug therapy， endoscopic resection， and regular surveillance. Although these disease management strategies are relatively mature， they present limitations in early lesion prevention and recurrence risk control. Therefore， it is imperative to identify therapeutic approaches for CAG and PLGC that offer preventive， reversible， and recurrence-reducing benefits. With advances in research on the mechanisms underlying inflammation-cancer transformation and the integration of traditional Chinese and Western medicine， the advantages of TCM in preventing and even reversing early-stage CAG and PLGC have gradually become apparent. This review explored the mechanisms of inflammation-cancer transformation in CAG from five aspects： inflammatory microenvironment， autophagy， glycolysis， bile acids， and ferroptosis. In conjunction with TCM theory and a deeper understanding of the distinct mechanisms involved in the inflammation-cancer transformation of CAG， this review further discussed the specific mechanisms through which TCM intervened in treating CAG and PLGC， with the aim of providing theoretical support and therapeutic insights for future clinical applications.关键词：chronic atrophic gastritis;inflammation-cancer transformation;pathogenesis;traditional Chinese medicine;review5|0|0更新时间：2025-07-10
- “在“第二十七期临床优势病种系列（类风湿关节炎）青年沙龙”上，近20位专家学者就RA的现代医学临床需求及中医药优势阶段与环节等方面展开了积极的讨论。中西医结合治疗RA在疾病活动期、难治性RA及严重并发症阶段具有显著优势，能迅速控制病情、减轻症状、提高生活质量并促进康复。未来，中西医结合的个体化治疗策略将成为提升RA患者生活质量的重要方向。”摘要：Rheumatoid arthritis （RA） is an autoimmune disease characterized primarily by erosive arthritis， with a high prevalence and disability rate. Although significant progress has been made in the treatment of RA in recent years， challenges such as suboptimal efficacy， drug resistance， severe side effects， and high costs of long-term treatment remain， especially for patients in the early stages of RA， as well as those with RA complications， comorbidities， and severe conditions. Hosted by the China-Japan Friendship Hospital and organized by the Youth Committee of the China Association of Chinese Medicine， the 27th session of the Clinical Dominant Disease Series （Rheumatoid Arthritis） Youth Salon invited nearly 20 experts and scholars from traditional Chinese medicine （TCM）， Western medicine， and interdisciplinary fields to actively discuss the clinical needs of modern medicine and the advantageous stages and aspects of TCM in RA. Experts at the salon agreed that TCM has unique advantages in the treatment of RA， especially during the early stage， periods of low to moderate disease activity， remission phase， and in addressing complications and comorbidities. TCM can achieve both prevention and treatment by regulating the immune system and restoring immune homeostasis. The integrated approach of traditional Chinese and Western medicine demonstrates significant advantages in active RA， refractory cases， and stages with severe complications， by rapidly controlling disease progression， alleviating symptoms， enhancing the quality of life， and facilitating recovery. Given the frequent occurrence of multiple comorbidities in RA， TCM shows potential in regulating immunity， alleviating symptoms， and improving physical constitution， which provides new insights into the comprehensive treatment of RA with comorbidities. However， high-quality clinical studies on integrated traditional Chinese and Western medicine in RA are still lacking. It is necessary to establish large-scale clinical cohorts and biological databases to provide a scientific basis for the development of precision-targeted therapies and clinical treatment protocols. In the future， individualized treatment strategies integrating traditional Chinese and Western medicine are expected to become an important direction for improving the quality of life in RA patients.关键词：rheumatoid arthritis;clinical dominant disease;traditional Chinese medicine;integrated traditional Chinese and western medicine;expert recommendation5|0|0更新时间：2025-07-10
- “最新研究发现，沉默lncRNA MANCR可通过调节miR-150-5p/GPNMB轴抑制胃癌细胞迁移、增殖和EMT。”摘要：ObjectiveTo investigate the effects of mitotically-associated long non-coding RNA （lncRNA MANCR） on the proliferation，migration， and epithelial mesenchymal transition （EMT） of gastric cancer （GC） cells by regulating the microRNA-50-5p （miR-150-5p）/non-metastatic melanoprotein B （GPNMB） axis.MethodsThe mRNA expressions of lncRNA MANCR，miR-150-5p， and GPNMB in 42 cases of GC tissue and adjacent tissue resected during surgery in the First Hospital of Hebei Medical University from June 2022 to September 2023 were detected by Real-time PCR. Human gastric mucosal epithelial cells GES-1 and human GC cells BGC-823 were cultured in vitro， and their lncRNA MANCR expression was detected. BGC-823 cells were randomly separated into control group （routine culture），sh-NC group （with sh-NC transfected），sh-MANCR group （with sh-MANCR transfected），sh-MANCR + anti-NC group （with sh-MANCR and anti-NC both transfected），and sh-MANCR + anti-miR-150-5p group （with sh-MANCR and anti-miR-150-5p both transfected）. The mRNA expressions of lncRNA MANCR，miR-150-5p， and GPNMB in the BGC-823 cells of all groups were analyzed. EdU staining was used to detect the proliferation of BGC-823 cells. Transwell assay was used to detect the migration and invasion of BGC-823 cells. The expressions of EMT-related proteins E-cadherin，N-cadherin，Vimentin， and GPNMB were detected by Western blot. The interactions between lncRNA MANCR and miR-150-5p and between miR-150-5p and GPNMB were analyzed by dual luciferase reporter assay.ResultsThe mRNA expressions of lncRNA MANCR and GPNMB in GC tissue were higher than those in adjacent tissue，and the expression of miR-150-5p was lower than that in adjacent tissue （P<0.05）. Compared with that in GES-1，lncRNA MANCR expression in BGC-823 cells was increased （P<0.05）. Compared with those in the sh-NC group and control group，the EdU-positive cell rate，migration number，invasion number，the mRNA expressions of lncRNA MANCR and GPNMB， and the expressions of protein，N-cadherin protein， and Vimentin protein in the BGC-823 cells in the sh-MANCR group were lower ，and the protein expressions of miR-150-5p and E-cadherin were higher （P<0.05）. Compared with those in the sh-MANCR group and the sh-MANCR + anti-NC group，the protein expressions of miR-150-5p and E-cadherin in the sh-MANCR + anti-miR-150-5p group were decreased. The EdU-positive cell rate，migration number，invasion number，mRNA expressions of GPNMB， and expressions of protein，N-cadherin protein， and Vimentin protein were increased （P<0.05）. lncRNA MANCR could target the negative regulation of miR-150-5p，and miR-150-5p could target the negative regulation of GPNMB.ConclusionKnockout of lncRNA MANCR can inhibit the proliferation，migration， and EMT of GC cells by regulating the miR-150-5p/GPNMB axis.关键词：mitotically-associated long non-coding RNA;microRNA-50-5p;non-metastatic melanoprotein B;gastric cancer;proliferation;migration;epithelial mesenchymal transition5|0|0更新时间：2025-07-10
- “最新研究发现，中医药调控内质网应激治疗溃疡性结肠炎具有独特优势，为治疗提供新思路。”摘要：Ulcerative colitis （UC） is a chronic and relapsing inflammatory bowel disease that primarily affects the mucosal layer of the rectum and colon. Its pathogenesis is complex and remains incompletely understood. Endoplasmic reticulum stress （ERS） plays a critical role in cellular responses to external stress and the maintenance of homeostasis， and its abnormal activation is closely associated with the development of various inflammatory diseases， particularly in the pathological process of UC. ERS maintains cellular homeostasis by activating the unfolded protein response （UPR）. However， when ERS is prolonged or excessive， UPR fails to alleviate the stress， leading to epithelial cell death and aggravating the progression of UC. Modulating ERS may serve as a key target for the prevention and treatment of UC， and it is one of the current research hotspots. Traditional Chinese medicine （TCM） has shown significant efficacy in regulating ERS， offering unique therapeutic advantages through multi-target and multi-pathway mechanisms. Recent studies have confirmed that TCM can alleviate ERS， inhibit apoptosis， regulate autophagy， reduce inflammatory responses， and maintain intestinal barrier function to prevent and treat UC. This review summarized the relationship between ERS and UC and discussed the intervention of TCM in regulating ERS for the treatment of UC， aiming to provide new insights and approaches for the treatment of UC with TCM.关键词：traditional Chinese medicine;ulcerative colitis;endoplasmic reticulum stress;apoptosis;autophagy6|0|0更新时间：2025-07-10
- “在心脾两虚型失眠研究领域，专家通过多数据库检索，发现现有动物模型存在不足，提出了改进方式及思路，为构建更贴合临床实际的模型提供解决方案。”摘要：Heart and spleen deficiency syndrome is the most common syndrome type in patients with insomnia. Based on the theory of disease syndrome-combined animal model， this paper used multiple databases to search for the keywords "heart and spleen deficiency"， "insomnia"， "sleepless"， "disease syndrome-combined animal model"， "model evaluation"， etc. It selected the literature related to the animal model of insomnia with heart and spleen deficiency in the past 20 years to evaluate from the aspects of model establishment， modeling factors， syndrome model， disease model， macro characterization & macro characterization evaluation scale， micro indicators， etc. It is found that the existing animal model of insomnia with heart and spleen deficiency is not completely constructed by the method of disease syndrome combination of disease modeling factors and syndrome modeling factors. In the model using this method， the single establishment factor of heart and spleen deficiency does not conform to the clinical reality of disease， and the selection of the factors for the insomnia model is not closely related to or even separated from the syndrome performance. There is a problem of insufficient quantification of macro representation when the macro representation of the model replaces the symptoms related to heart and spleen deficiency syndrome and insomnia in an equivalent manner for macro representation evaluation， which can be improved according to the quantitative ideas and examples of the existing macro representation and macro representation evaluation scale. There are few specific indicators of heart and spleen deficiency syndrome in micro indicators. The micro research of heart and spleen deficiency syndrome and the essence of other traditional Chinese medicine （TCM） syndromes can be carried out by metabonomics and other technologies combined with the theory of corresponding prescription and syndrome， along the specific related ideas of "prescription and syndrome， treatment principle and selection of prescription， treatment principle and selection of acupoints， as well as therapeutic mechanism and syndrome essence". The future users and researchers of animal models of insomnia with heart and spleen deficiency can get improved methods and ideas through the shortcomings of animal models of heart and spleen deficiency listed in this paper and construct animal models of insomnia with heart and spleen deficiency that are more suitable for clinical practice， so as to establish a more perfect modeling method and evaluation system of disease syndrome-combined animal model.关键词：heart and spleen deficiency;insomnia;sleepless;disease syndrome-combined animal model;model evaluation14|6|0更新时间：2025-07-01
- “在类风湿性关节炎治疗领域，中药黄酮类化合物因其抗炎、抗氧化、免疫调节等生物活性受到关注，有望成为潜在药物，为新药研发提供新思路。”摘要：Rheumatoid arthritis （RA） is a chronic autoimmune disease characterized by synovitis as its pathological basis. Although current therapeutic drugs can alleviate symptoms， they are often accompanied by a high risk of side effects. In recent years， the use of flavonoids from traditional Chinese medicine （TCM） in the treatment of RA has garnered significant attention. Studies have shown that the mechanisms by which flavonoids treat RA include inhibiting the release of pro-inflammatory factors， regulating multiple cellular signaling pathways， alleviating oxidative stress， modulating immune system functions， inhibiting bone destruction， and suppressing angiogenesis. Due to their notable anti-inflammatory， antioxidant， and immunomodulatory activities， flavonoids hold promise as potential therapeutic agents for RA. A substantial number of articles in this field have been published. By reviewing domestic and international literature and applying bibliometric and visual analysis using CiteSpace， this paper aims to explore research hotspots and frontiers in this field， systematically review the structures and anti-RA mechanisms of TCM flavonoids， provide a theoretical basis for their use in RA treatment and clinical applications， and offer new perspectives and references for the discovery of novel TCM-based anti-RA drugs.关键词：flavonoid compounds;rheumatoid arthritis;mechanism of action;signaling pathways;fibroblast-like synoviocytes13|10|0更新时间：2025-07-01
- “在中药质量控制领域，专家建立了恒制咳喘胶囊多成分指纹图谱及含量检测方法，为提升该药品质量控制提供科学指导。”摘要：ObjectiveThe fingerprints of 15 batches’ Hengzhi Kechuan capsules were established， and to quantitatively analyze the ten index components. Furthermore， the uniformity of samples from different batches of differnet years by Heat Map Clustering Analysis.MethodsThe fingerprints of Hengzhi Kechuan capsules were established by a combination method of high performance liquid phase chromatography with UV detector and electrospray detector （HPLC-DAD-CAD） to quantitatively analyze the index components. Adenosine， guanosine， vanillic acid， safflomin A， agrotetrol， naringin， hesperidin， militarine， ginsenoside， ginsenoside Rb1 and glycyrrhizic acid were selected as quality attribute indexes. A total of 15 batches of Hengzhi Kechuan capsules from 2022-2024 were quantitatively analyzed， and the homogeneity and difference of quality attribute indexes in different years were analyzed by Heat Map clustering Analysis.ResultsHPLC fingerprints of Hengzhi Kechuan capsules were established. The HPLC analysis methods could be applied to quantitative analysis of HZKC capsules. The above methods were used to analyze the content of 15 batches of samples. The concentrations in HZKC capsules ranged from 0.038-0.078 mg/g for adenosine， 0.115-0.251 mg/g for guanosine， 0.007-0.018 mg/g for vanillic acid， 0.291-0.673 mg/g for safflomin A， 0.122-0.257 mg/g for agrotetrol， 0.887-1.905 mg/g for naringin， 1.841-3.364 mg/g for hesperidin， 1.412-2.450 mg/g for militarine， 2.207-3.112 mg/g for ginsenoside Rb1 and 0.650-1.161 mg/g for glycyrrhizic acid. The concentrations of ginsenoside Rb1 and glycyrrhizic acid in fifteen batches all met the requirements in Chinese Pharmacopoeia （2020 edition）. For the samples from 15 batches， the intra-batch consistency （PA） values were between 2.57%-9.28%， suggesting that the production process parameters of the manufacturers were stable and consistent. The inter-batch consistency （PB） value from the manufacture were between 33.86%-92.97%. The variation of individual components between batches were found to be highly correlated with the year and represented by Heat Map Clustering Analysis.ConclusionA multi-component fingerprint was established， and a reliable multi-component high-performance liquid chromatography method was established， which showed that the manufacturer had excellent intra-batch consistency， and distinguished the samples of different years through the variation in the content of each index component， indicating that partial chemical materials feeding was related to the year.关键词：Hengzhi Kechuan Capsules;HPLC-DAD-CAD;quality uniformity;fingerprint;Heat Map clustering50|18|0更新时间：2024-12-18
- “最新研究发现，仙连解毒方通过调节肠道菌群，增强CD8+ T细胞的抗肿瘤免疫反应，对结直肠癌具有显著治疗效果。”摘要：ObjectiveTo investigate the effect of Xianlian Jiedu prescription in modulating antitumor immune response of intestinal flora-mediated CD8+ T cells in colorectal cancer （CRC）.MethodThirty male BALB/c mice were divided into a normal group， a model group， an oxaliplatin group （8×10-3 g·kg-1·3 d-1）， a high-dose Xianlian Jiedu prescription （XLJD-H） group （12.9 g·kg-1·d-1）， and a low-dose Xianlian Jiedu prescription （XLJD-L） group （6.45 g·kg-1·d-1）. A CRC xenograft model was established through subcutaneous injection of CT-26 cells， followed by respective interventions. Additionally， ten male BALB/c mice were divided into a Xianlian Jiedu prescription-fecal microbiota transplantation （XFMT） group and a model-fecal microbiota transplantation （MFMT） group. The intestinal colonized bacteria were eradicated with a quadruple antibiotic solution， and then a pseudo-germ-free CRC xenograft mouse model was established through subcutaneous injection of CT-26 cells. Fecal samples from the model group and XLJD-H group were collected to prepare fecal microbiota solutions. The XFMT group was gavaged with the fecal microbiota solution from CRC mice treated with XLJD-H， and the MFMT group received the fecal microbiota solution from CRC mice in the model group. Tumor volume changes were monitored. Hematoxylin and eosin （HE） staining was used to assess pathological and morphological changes of the tumor. PacBio full-length diversity sequencing was used to analyze intestinal flora changes. Flow cytometry and immunofluorescence staining were employed to evaluate changes in T cell infiltration in tumor tissue. Enzyme-linked immunosorbent assay （ELISA） was performed to measure differences in IFN-γ levels in tumor tissue.ResultCompared to those in the model group， the tumor volumes in the oxaliplatin group， XLJD-H group， and XLJD-L group were significantly reduced （all P<0.01）. H&E staining revealed that compared to the model group， the oxaliplatin and XLJD-H groups exhibited an increased necrotic area within the tumor tissue， with a sparser cellular arrangement and reduced nuclear division figures. Intestinal flora sequencing analysis indicated that although the Chao1， ACE， Shannon， and Simpson indices in the XLJD-H group were reduced compared to the model group， the differences were not statistically significant （all P>0.05）. Principal co-ordinates analysis （PCoA） demonstrated a distinct difference in the intestinal flora structure between the normal group and model group. Moreover， the intestinal flora structure of CRC mice in the XLJD-H group was significantly more similar to that of the normal group （P<0.01）. Compared to that in the control group， the abundance of Akkermansia muciniphila， Muribaculum intestinale， Parabacteroides goldsteinii， Bacteroides thetaiotaomicron， and Parabacteroides gordonii significantly decreased in the model group was significantly dropped （P<0.05， P<0.01）， while the abundance of Duncaniella muris， Prevotellamassilia sp002933955， Prevotella sp002933775， and Kineothrix alysoides was significantly increased （P<0.05， P<0.01）. Furthermore， compared to that in the model group， the abundance of Akkermansia muciniphila significantly increased in the XLJD-H group （P<0.05）， whereas the abundance of Kineothrix alysoides significantly decreased （P<0.01）. Linear discriminant analysis （LDA>4.0） revealed an enrichment of Alistipes， Helicobacteraceae， Helicobacter， Helicobacter pylori BU， Bacteroides acidifaciens， Campylobacterales， Kineothrix， Kineothrix alysoides， Acetatifactor sp 900066365， Faecalimonas， Faecalimonas umbilicata， Acetatifactor， Lachnospiraceae， Clostridia， Eubacteriales， and Firmicutes in the model group， whereas Verrucomicrobia， Verrucomicrobiales， Akkermansiaceae， Akkermansia， Akkermansia muciniphila， Bacteroidales， Bacteroidia， Bacteroidetes， Parabacteroides， Tannerellaceae， Muribaculum， and Parabacteroides gordonii were enriched in the XLJD-H group. Additionally， compared to the model group， the XLJD-H group exhibited a significant increase in the proportion of CD8+ T cells and a higher level of IFN-γ in tumor tissue （P<0.01）， while the proportion of CD4+ T cells in the tumor tissue of the XLJD-H group was significantly reduced （P<0.05）. Moreover， compared to the MFMT group， the XFMT group exhibited a significant reduction in xenograft tumor volume （P<0.05）. HE staining indicated an increase in necrotic areas within the tumor tissue of the XFMT group， along with a sparser arrangement of cells and a reduction in nuclear division. Compared to the MFMT group， the XFMT group showed a significant decrease in both Shannon and Simpson indices （P<0.05， P<0.01）. PCoA suggested a distinct difference in the intestinal flora structure between the MFMT and XFMT groups （P<0.01）. At the species level， compared to the MFMT group， the abundance of Akkermansia muciniphila and Phocaeicola sartorii significantly increased in the XFMT group （both P<0.01）. Linear discriminant analysis （LDA>4.0） results indicated enrichment of Muribaculum sp002492595， Acetatifactbr sp900066365， Kineothrix， Duncaniella freteri， Oscillospiraceae， Acetatifactor， Faecalimonas umbilicata， Faecalimonas， Bacteroidia， Bacteroidales， Lachnospiraceae， Clostridia， Eubacteriales， and Firmicutes in the MFMT group， whereas Verrucomicrobia， Verrucomicrobiae， Verrucomicrobiales， Akkermansiaceae， Akkermansia， Akkermansia muciniphila， Phocaeicola sartorii， and Phocaeicola were enriched in the XFMT group. Compared to the MFMT group， the XFMT group exhibited a significant increase in the proportion of CD8+ T cells and a higher level of IFN-γ in tumor tissue （P<0.01）.ConclusionThe Xianlian Jiedu prescription may ameliorate dysbiosis in the intestines of CRC mice and enhance the antitumor immune response mediated by CD8+ T cells， thereby exerting an anticancer effect against CRC.关键词：intestinal flora;tumor immunity;colorectal cancer;Xianlian Jiedu prescription;traditional Chinese medicine45|5|0更新时间：2024-08-12
- “最新研究进展：专家基于UPLC-Q-TOF-MS/MS技术，快速识别保元汤化学成分，明确其物质基础，为后续研究提供参考。”摘要：ObjectiveA rapid method for identification of chemical constituents in Baoyuantang reference sample was established in order to clarify the material basis.MethodBased on UPLC-Q-TOF-MS/MS technology and self-established database information， the chemical components in Baoyuantang were systematically characterized and identified. The chromatography was performed on A Waters ACQUITY UPLC HSS T3 （2.1 mm×100 mm， 1.8 μm） column with mobile phase consisting of 0.1% formic acid water （A）-0.1% formic acid acetonitrile （B） for gradient elution （0~3 min， 2%~19%B； 3~8 min， 19%B； 8~8.1 min， 19%~22%B； 8.1~14 min， 22%~29%B； 14~16 min， 29%B； 16~32 min， 29%~45%B； 32~32.1 min， 45%~90%B； 32.1~35 min， 90%~95%B； 35~36 min， 95%~98%B； 36~37 min， 98%~2%B； 37~40 min， 2%B）， flow rate 0.5 ml·min-1， column temperature 35 ℃. Continuum data format was collected in both positive and negative ion modes，with a quality scanning range of m/z 50~1 500.ResultA total of 322 components were identified under negative ion mode and 181 under positive ion mode， including 116 triterpene saponins， 66 flavonoids， 19 organic acids， 6 gingerphenols， 6 gingerols， 5 gingerones， 10 amino acids， 7 sugars， 5 coumarins and 82 other components after weight removal. Among them， ginseng contains 83 components， licorice contains 141 components， Astragalus contains 39 components， cinnamon contains 35 components， and ginger contains 38 components.ConclusionIn this study， the rapid characterization and identification of multi-class components in Baoyuantang was realized， and it was confirmed that the substance base of Baoyuantang was mainly triterpenoid saponins， flavonoids， gingerols and organic acids， and the chemical composition was attributed and analyzed， which provided a reference for the subsequent quality control and material basic research.关键词：classics famous prescription;Baoyuantang;chemical component;ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry（UPLC-Q-TOF-MS/MS）;structural characterization55|22|0更新时间：2024-08-05
- 摘要：ObjectiveEvaluate clinical effect of astragalus formula diet combined with piracetam on Yin deficiency and Yang hyperactivity after hypertensive cerebral hemorrhage and impact on expression of tumor necrosis factor related apoptosis inducing ligand （TRAIL） and transforming growth factor-β1（TGF-β1）.MethodsSeventy patients with hypertensive intracerebral hemorrhage who underwent minimally invasive drainage of hematoma through a soft-channel were enrolled during June 2021 to December 2022，and 1∶1 randomly divided into control group and observation group. The control group was given piracetam after operation，while observation group was additionally given astragalus formula diet as nutritional support. Both groups were treated continuously for 3 weeks.the National Institutes of Health Stroke Scale （NIHSS） score scale was used to evaluate the neurological impairment of the patients before and after treatment，and the Barthel index scale was used to evaluate the daily living ability of the patients. Serum samples of the patients were collected before and after treatment and endothelin-1 （NO） levels were detected by nitrate reductase method，endothelin-1 （ET-1） levels were detected by radioimmunoassay，TRAIL and TGF-β1 levels were detected by enzyme-linked immunosorbent assay. Traditional Chinese medicine（TCM）syndrome score and clinical efficacy were compared between the two groups.ResultAfter treatment，the total effective rate of the observation group was higher than that of the control group，the postoperative recovery time was shorter than that of the control group （P<0.05），and the improvement of TCM syndrome score was better than that of the control group （P<0.01）. Compared with before treatment，NIHSS was decreased and Barthel score was increased in both groups after treatment （P<0.01）. Compared with control group，NIHSS score was lower and Barthel score was higher in observation group after treatment （P<0.01）. Compared with before treatment，the expression levels of ET-1 and TRAIL in both groups were decreased after treatment，and compared with control group，the expression levels of ET-1 and TRAIL in observation group were decreased （P<0.01）. Compared with before treatment，the expression levels of NO and TGF-β1 were increased after treatment，and compared with the control group，the expression levels of NO and TGF-β1 were increased in the observation group （P<0.01）. During treatment，there was no difference in the total incidence of adverse reactions between the two groups.ConclusionAstragalus formula diet nutritional support combined with piracetam can effectively improve the syndrome，neurological deficit，daily living function and vascular endothelial function damage in patients with hypertensive cerebral hemorrhage，and does not increase the incidence of adverse reactions.关键词：hypertensive cerebral hemorrhage;Yin deficiency and Yang hyperactivity syndrome;Astragalus formula diet nutrition support;tumor necrosis factor related apoptosis inducing ligand （TRAIL）;transforming growth factor-β1（TGF-β1）37|5|0更新时间：2023-10-10
- 摘要：Epilepsy is a common nervous system disease， which is characterized by repeated attacks， prolonged and difficult to heal， causing great harm to the patient's body and mind.Antiepileptic drugs bring good therapeutic effect， but also accompanied by a lot of physical and mental damage of toxic side effects.Traditional Chinese medicine has a long history in the treatment of epilepsy. At present， in addition to enriching the cognitive theory of traditional Chinese medicine in the treatment of epilepsy， the research on the cell signal transduction mechanism of traditional Chinese medicine in the treatment of epilepsy from the perspective of molecular biology is developing rapidly.Through literature search at home and abroad， it is found that epilepsy is closely related to cell proliferation， apoptosis， autophagy， inflammatory response， immune response and other pathophysiological processes.At the same time， the modern medical research of traditional Chinese medicine and western medicine shows that there is a direct or indirect relationship between the study on the mechanism of the treatment of epilepsy by monomer Chinese medicine， single Chinese medicine or even compound Chinese medicine.They can inhibit the occurrence of epilepsy， control epilepsy and protect the brain injury caused by epilepsy by regulating the expression of key molecules in the corresponding signaling pathway.In this paper， the research status at home and abroad is summarized as follows：①Tangeretin， ginkgolide B and other drugs can inhibit apoptosis and oxidative stress by activating PI3K/ Akt signaling pathway.②Baicalin，osthol and other drugs can inhibit autophagy by inhibiting mTOR signaling pathway.③Ganoderma polysaccharides， astragaloside and other drugs can reduce cell apoptosis by inhibiting MAPK signaling pathway.④Salidroside，resveratrol and other drugs can inhibit oxidative stress and apoptosis by activating Nrf2/ARE/HO-1 signaling pathway.⑤Curcumin， baicalin and other drugs can reduce inflammation and apoptosis by inhibiting NF-κB signaling pathway.The above summary aims to provide a reference for the in-depth study of the treatment of epilepsy with traditional Chinese medicine， and also provide a new idea for the clinical treatment of epilepsy with traditional Chinese medicine.关键词：epilepsy;traditional Chinese medicine;signaingl pathway;research progress245|54|0更新时间：2021-07-20
- Postal code：100700
- Tel：010-84076882 Email：syfjx_2010@188.com
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0