最新刊期
- “最新研究发现，开玄解毒方能有效改善银屑病小鼠皮肤病理损伤，其机制可能与调节铁死亡相关基因CHAC1、ALOX12B、TRIM21的表达密切相关。”摘要：ObjectiveTo use bioinformatics technology to screen the molecular patterns and diagnostic biomarkers of ferroptosis closely related to psoriasis， observe the therapeutic effect of Kaixuan Jiedu core prescription on psoriasis and explore its potential mechanism through animal experiments.MethodsPsoriasis microarray data from GEO were analyzed to identify differentially expressed genes （DEGs）. Intersection with a ferroptosis gene set yielded psoriasis ferroptosis-related genes （FRGs）， which underwent correlation， consensus clustering， enrichment， and immune infiltration analyses. Core diagnostic FRGs （Hub-FRGs） were identified using random forest （RF）， support vector machine （SVM）， LASSO regression， Nomogram， and ROC analyses. In vivo， imiquimod （5% cream） induced psoriasis in mice （except controls）. Drug treatment groups received respective doses， while control and model groups received saline via daily gavage for 7 days. Back skin changes were recorded and PASI scored. HE staining assessed histopathology. The levels of ferrous ion （Fe2+）， malondialdehyde （MDA）， 4-hydroxynonenal （4-HNE） and free fatty acid （FFA） in skin tissue were detected. The level of reactive oxygen species （ROS） in skin tissue was detected by immunofluorescence. Immunohistochemistry was used to detect the expression of ChaC glutathione-specific γ-glutamyl transferase 1 （CHAC1）， arachidonic acid 12-lipoxygenase β （ALOX12B）， tri-motif protein 21 （TRIM21）， proliferation marker （Ki-67） and nuclear transcription factor-κB （NF-κB） protein.ResultsAnalysis of GSE30999 identified 2100 DEGs and 24 FRGs. Gene Ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） enrichment revealed 1 000 biological functions and 75 pathways. After cluster analysis， combined with three machine learning algorithms， Nomogram and ROC curve analysis， the core Hub-FRGs （CHAC1， ALOX12 B， TRIM21） were obtained. Immunoinfiltration showed inactive memory CD4+T cells and activated dendritic cells abundance significantly correlated with Hub-FRGs. In vivo， model group vs. control showed significantly increased PASI/Baker scores （P<0.05）， epidermal hyperkeratosis， inflammatory infiltration， and elevated levels of Fe2+， MDA， 4-HNE， FFA， ROS， CHAC1， ALOX12B， TRIM21， Ki-67， and NF-κB （P<0.05）. Drug groups vs. model group exhibited significantly reduced scores （P<0.05）， alleviated skin lesions， and decreased levels of Fe2+， MDA， 4-HNE， FFA， ROS， Hub-FRGs， Ki-67， and NF-κB （P<0.05）.ConclusionKaixuan Jiedu core prescription can significantly improve the skin pathological injury of psoriasis mice， showing good therapeutic and repair effects， and its mechanism may be related to regulating the expression of ferroptosis genes CHAC1， ALOX12B and TRIM21， which are closely related to the pathogenesis of psoriasis.关键词：psoriasis;Kaixuan Jiedu core prescription;ferroptosis;bioinformatics;machine learning;mice6|0|0更新时间：2025-07-18
- “最新研究发现，开玄-解毒配伍能有效减轻银屑病症状，保护皮肤屏障，且安全性良好。”摘要：ObjectiveTo investigate the efficacy and safety of Kaixuan Jiedu compatibility and the decomposed prescriptions in the treatment of psoriasis.MethodsThirty Balb/c mice were randomly grouped as follows （n=6）： normal， model， Kaixuan Jiedu （KXJD， 15.21 g·kg-1）， Kaixuan （KX， 3.08 g·kg-1）， and Jiedu （JD， 12.13 g·kg-1）. Except the normal group， the rest groups were modeled for psoriasis-like skin lesions by topical application of imiquimod， and samples were collected after 7 days of continuous intervention. Mice were photographed at the lesion site during modeling and before sampling and the psoriasis area and severity index （PASI） was calculated. Hematoxylin-eosin （HE） staining was used to observe pathological changes in the lesions and measure the epidermal thickness. Mice were photographed and observed for the tortuous dilation of dermal capillaries. The expression of vascular endothelial growth factor （VEGF）， platelet-endothelial cell adhesion molecule （CD31）， proliferating cell nuclear antigen （Ki67）， and cytokeratin 10 （CK10） in the epidermal tissue was detected by immunohistochemistry. Immunofluorescence assay was employed to determine the expression of Claudin-1 and Occludin. Real-time PCR was employed to determine the mRNA levels of interleukin-17A （IL-17A） and interleukin-23 （IL-23）. The spleen and thymus were photographed and weighed， and the spleen and thymus indices were calculated. The safety of the treatment was assessed by automatic biochemistry testing of the serum， liver， and kidney functions and by HE staining of the liver， kidney and spleen.ResultsCompared with that of the normal group， the skin of the model group showed erythema， infiltration， and typical psoriasis-like changes， tortuous dilation of dermal capillaries， hyperkeratosis in epidermal cells， acanthosis， massive lymphocytic infiltration in the dermis， impaired barrier function， increased expression of VEGF， CD31， Ki67， and CK10 （P<0.01）， reduced expression of Claudin-1 and Occludin （P<0.01） in the epidermis， and up-regulated mRNA levels of IL-17A and IL-23 （P<0.01）. In addition， the mice in the model group showed spleen enlargement， thymus atrophy， increased spleen index， and decreased thymus index （P<0.01）. Compared with the model group， KXJD and JD reduced psoriasis-like skin lesions， inhibited the tortuous dilation of dermal capillaries， reduced the expression of VEGF， CD31， Ki67， and CK10 （P<0.01）， increased the expression of claudin-1 （P<0.01）， and down-regulated the mRNA levels of inflammatory factors （P<0.01）. Moreover， the KXJD group outperformed the JD group. The JD group showed no significant difference from the model group regarding the spleen index， thymus index， and Occludin expression. The psoriasis indicators in the KX group were not significantly different from those in the model group.ConclusionKXJD and JD can reduce the symptoms of local skin lesions of psoriasis， which is manifested as different inhibition degrees of the proliferation and differentiation of keratin-forming cells， tortuous dilation of dermal capillaries， and inflammatory reactions， as well as the protection of the skin barrier. Moreover， KXJD outperformed JD. KX alone did not significantly reduce psoriasis lesions in mice. KXJD and the decomposed prescriptions are safe and effective， causing no obvious liver and kidney injuries.关键词：Kaixuan Jiedu core prescription;psoriasis;Kaixuan;Jiedu;safety evaluation2|0|0更新时间：2025-07-18
- “据最新研究，中药黄酮类化合物在治疗类风湿性关节炎领域显示出显著抗炎、抗氧化、免疫调节等生物活性，有望成为潜在药物，为临床治疗提供新思路。”摘要：Rheumatoid arthritis （RA） is a chronic autoimmune disease characterized by synovitis as its pathological basis. Although current therapeutic drugs can alleviate symptoms， they are often accompanied by a high risk of side effects. In recent years， the use of flavonoids from traditional Chinese medicine （TCM） in the treatment of RA has garnered significant attention. Studies have shown that the mechanisms by which flavonoids treat RA include inhibiting the release of pro-inflammatory factors， regulating multiple cellular signaling pathways， alleviating oxidative stress， modulating immune system functions， inhibiting bone destruction， and suppressing angiogenesis. Due to their notable anti-inflammatory， antioxidant， and immunomodulatory activities， flavonoids hold promise as potential therapeutic agents for RA. A substantial number of articles in this field have been published. By reviewing Chinese and international literature and applying bibliometric and visual analysis using CiteSpace， this paper explored research hotspots and frontiers in this field， systematically reviewed the structures and anti-RA mechanisms of TCM flavonoids， provided a theoretical basis for their use in RA treatment and clinical applications， and offered new perspectives and references for the discovery of novel TCM-based anti-RA drugs.关键词：flavonoid compound;rheumatoid arthritis;mechanism of action;signaling pathway;fibroblast-like synoviocyte26|16|0更新时间：2025-07-18
- “最新研究发现，菟丝子-枸杞子能通过调控SIRT1/Nrf2通路，抑制氧化应激，改善血睾屏障损伤，提高少弱精子症大鼠生精功能。”摘要：ObjectiveTo investigate the effect of the herb pair Cuscutae Semen-Lycii Fructus on oxidative stress-induced blood-testis barrier dysfunction and spermatogenesis in the rat model of oligoasthenozoospermia （OAS） and decipher the mechanism based on the silent information regulator 1 （SIRT1）/nuclear factor erythroid 2-related factor 2 （Nrf2） signaling pathway.MethodsThirty-five male SD rats were randomized into a blank group （n=7） and a modeling group （n=28）. The OAS model was established by gavage of hydrocortisone aqueous solution combined with single factor electrical stimulation. The modeled rats were randomly assigned into the following groups： model， Cuscutae Semen-Lycii Fructus granules （3.2 g·kg-1）， Cuscutae Semen-Lycii Fructus total flavonoids （0.34 g·kg-1）， and L-carnitine （0.38 g·kg-1）， and treated for 4 weeks. The sperm quality of rats was assessed by an automatic sperm analyzer. The levels of superoxide dismutase （SOD）， malondialdehyde （MAD）， and glutathione peroxidase （GSH-Px） in the testicular tissue were determined by enzyme-linked immunosorbent assay. Hematoxylin-eosin staining was employed to reveal the pathological changes in the testicular tissue and score the spermatogenic function. Transmission electron microscopy was employed to observe the ultrastructural changes of Sertoli cells. Western blot and Real-time PCR were employed to determine the protein and mRNA levels， respectively， of SIRT1， Nrf2， Occludin， zonula occludens-1 （ZO-1）， connexin 43 （CX43）， and β-catenin.ResultsCompared with the blank group， the model group showed decreased total sperm count and motility （P<0.05， P<0.01）， obvious damage in the testicular tissue and blood-testis barrier structure， reduced score of spermatogenic function （P<0.01）， declined levels of GSH-Px and SOD in the testicular tissue （P<0.05）， elevated level of MDA， and down-regulated protein levels of SIRT1， Nrf2， ZO-1， CX43， β-catenin， and occludin （P<0.05， P<0.01） and mRNA levels of SIRT1， Nrf2， ZO-1， CX43， and β-catenin in the testicular tissue （P<0.05， P<0.01）. After treatment， the testicular tissue， blood-testis barrier structure， and score of spermatogenic function （P<0.01） were improved in the Cuscutae Semen-Lycii Fructus granules group， Cuscutae Semen-Lycii Fructus total flavonoids group， and L-carnitine group. Compared with the model group， the treatment groups presented lowered levels of GSH-Px and SOD （P<0.05， P<0.01）， and the Cuscutae Semen-Lycii Fructus granule group showed a decline in MDA level. The protein and mRNA levels of SIRT1， Nrf2， ZO-1， CX43， β-catenin， and occludin were up-regulated in the Cuscutae Semen-Lycii Fructus granules group and total flavonoids group （P<0.05， P<0.01）.ConclusionThe herb pair Cuscutae Semen-Lycii Fructus can regulate the SIRT1/Nrf2 pathway to inhibit oxidative stress and alleviate the blood-testis barrier damage， thereby improving the spermatogenic function in the rat model of OAS. Total flavonoids may be the material basis for the therapeutic effect of Cuscutae Semen-Lycii Fructus.关键词：Cuscutae Semen-Lycii Fructus;oligoasthenozoospermia;blood-testis barrier;oxidative stress;silent information regulator/nuclear factor erythroid 2-related factor 22|0|0更新时间：2025-07-18
- “最新研究发现，开玄解毒核心方能调节银屑病样小鼠皮损中的鞘脂代谢，改善皮损，为银屑病治疗提供新思路。”摘要：ObjectiveTo explore the effects of Kaixuan Jiedu core prescription （KXJD） on sphingolipid metabolism in the mouse model of imiquimod-induced psoriasis-like skin lesions.MethodsThirty-seven male C57BL/6J mice were randomly assigned into five groups： healthy control （n=11）， model （n=11）， methotrexate （MTX， n=5）， low-dose （15.21 g·kg-1） KXJD （n=5）， and high-dose （30.42 g·kg-1） KXJD （n=5）. Psoriasis-like skin lesions were induced in mice with 62.5 mg 5% imiquimod cream applied on the back. The KXJD groups and MTX group were treated with 0.2 mL corresponding decoction and MTX， respectively， by gavage daily， while the other groups were given an equal volume of normal saline by the same way. After 5 days of treatment， back skin lesions were collected. Firstly， healthy control and model mice were selected for tandem mass tag （TMT） quantitative proteomics （control vs model=3 vs 3） and targeted lipid metabolomics （control vs model=11 vs 11）. Then， the binding degree between core components and target proteins was predicted via network pharmacology and molecular docking. Finally， an animal experiment was performed to decipher the specific regulation mechanism of KXJD on sphingolipid metabolism. Immunohistochemistry was employed to determine the expression level of sphingosine-1-phosphate （S1P）， and Western blot was employed to determine the expression levels of sphingosine kinase 2 （SPHK2） and monocyte chemotactic protein-1 （MCP-1）.ResultsTMT proteomics and targeted lipid metabolomics suggested that sphingolipid metabolism was active in the psoriatic skin， and key proteases ［serine palmitoyltransferase， long chain base subunit 2 （SPTLC2）， SPHK2， delta（4）-desaturase sphingolipid 1 （Degs1）， and ceramide synthase 4 （CerS4）］ and 8 sphingolipid metabolites （including ceramides， sphingol， sphingomyelin， and glycosphingolipid） expressed abnormally （P<0.05） compared with those in the healthy skin. The molecular docking results indicated that the binding energy between the active components （quercetin， kaempferol， and luteolin） in KXJD and key proteins involved in sphingolipid metabolism was less than -8 kal·mol-1. Further experimental verification showed elevated expression levels of SPHK2， S1P， and MCP-1 in psoriatic skin compared with healthy skin （P<0.05）， and KXJD down-regulated the expression levels of SPHK2， S1P， and MCP-1 compared with the model group （P<0.05）.ConclusionThis study indicates that there is an imbalance in sphingolipid metabolism in psoriatic skin lesions. KXJD may reduce psoriasis-like lesions in mice by regulating sphingolipid metabolism via the SPHK2/S1P/MCP-1 pathway.关键词：Kaixuan Jiedu core prescription;multi-omics analysis;sphingolipid metabolism;psoriasis vulgaris;sphingosine kinase 2 （SPHK2）/sphingosine-1-phosphate （S1P）/monocyte chemotactic protein-1 （MCP-1） pathway2|0|0更新时间：2025-07-18
- “在银屑病治疗领域，中医“怫郁”理论提供了新视角，通过解郁清热降低复发率，改善患者生活质量。”摘要：Psoriasis is a chronic inflammatory skin disease with a polygenic genetic background. Its etiology remains unclear， and its pathogenesis is complex and refractory， collectively posing significant challenges in its treatment and greatly affecting the physical and mental health of patients. With the advantages of multi-target and multi-pathway treatment， traditional Chinese medicine has shown unique efficacy and value in the diagnosis and treatment of psoriasis. Modern doctors have a lot of discussion on psoriasis， and most of them tend to treat the disease by solving disorders of the blood system. They think that the disease is closely related to the "heat in the blood". Combining the clinical characteristics and accompanying symptoms of psoriasis， this article traced the causes of "heat in the blood" in psoriasis and believed that multiple internal and external factors have prevented the smooth circulation of Qi. Yang Qi Fuyu （stagnation） and transformation into heat and toxicity is the source of "heat in the blood" in psoriasis. Furthermore， it was proposed that "Fuyu" is the core pathogenesis of psoriasis. The etiology of "Fuyu" is complex， such as external wind and cold pathogens， emotional injuries， internal accumulation of dampness， and deficiency of healthy Qi， all of which can disrupt the ascending and descending movement of Qi， impede the circulation of Qi and fluids， close the pores and skin texture， and subsequently lead to stagnation. Based on the above understanding， "resolving stagnation" is crucial for treating the disease. Many doctors have explored the treatment ideas of psoriasis from the perspectives of dispelling wind， warming cold， regulating Qi， eliminating dampness， tonifying deficiency， and external treatment， aiming to remove the causes， promote the circulation of Qi and fluids， and resolve stagnation and heat. Clinical studies have shown that the therapies can relieve clinical symptoms， reduce recurrence rate， and improve quality of life， which also have good safety in the treatment of psoriasis. This article discussed the treatment of psoriasis from the perspective of "Fuyu"， enriching the understanding of TCM regarding the etiology and pathogenesis of psoriasis. It is aiming to serve as an effective supplement to the "treating by solving disorders of the blood system" approach and provide a reference for clinical diagnosis and treatment of psoriasis.关键词：psoriasis;Fuyu;Liu Wansu;traditional Chinese medicine;theoretical discussion5|0|0更新时间：2025-07-15
- “最新研究发现，温阳散结加减方能有效抑制肺癌小鼠肿瘤生长，调节Th1/Th2平衡，为肺癌治疗提供新思路。”摘要：ObjectiveTo study the tumor inhibition and T helper cell （Th）1/Th2 balance regulation effect of modified Wenyang Sanjie prescription on lung cancer tumor-bearing mice and to elaborate its mechanism.MethodsA mouse model bearing a lung cancer tumor was established by subcutaneous injection of Lewis lung cancer cells into the armpit and was randomly divided into lung cancer model group， low-dose， medium-dose， and high-dose groups of modified Wenyang Sanjie Prescription， and positive control group， with 12 mice per group. The low-dose， medium-dose， and high-dose groups of modified Wenyang Sanjie Prescription were given modified Wenyang Sanjie Prescription by dosing at 2.5， 5， and 10 g·kg-1， once a day， respectively. The positive control group was intraperitoneally injected with cisplatin （2 mg·kg-1）， once every other day， for a total of 30 days. Serum Interferon （IFN）-γ， Interleukin （IL）-2， IL-4， IL-6， and IL-10 were determined by enzyme-linked immunosorbent assay （ELISA）， and spleen index， thymus index， and tumor growth inhibition rate were calculated. Tumor microvascular density was determined by immunohistochemistry， and tumor hypoxia inducible-factor （HIF）-1α， epidermal growth factor receptor （EGFR）， and vascular endothelial growth factor （VEGF） mRNA were determined by real-time quantitative polymerase chain reaction （PCR）. The protein levels of HIF-1α， EGFR， and VEGF were determined by Western blot.ResultsCompared with lung cancer model group， IFN-γ and IL-2 were increased in the modified Wenyang Sanjie Prescription groups and positive control group， while IL-4， IL-6， IL-10， spleen index， thymus index， tumor weight， and tumor microvascular density were decreased， as well as HIF-1α， EGFR， and VEGF mRNA and protein levels （P<0.05）. Compared to the low-dose group of modified Wenyang Sanjie prescription， IFN-γ and IL-2 were increased in the medium-dose and high-dose groups of modified Wenyang Sanjie Prescription， while IL-4， IL-6， IL-10， spleen index， thymus index， tumor weight， and tumor microvascular density were decreased， as well as HIF-1α， EGFR， and VEGF mRNA and protein levels （P<0.05）. IFN-γ and IL-2 were increased in the high-dose group of modified Wenyang Sanjie Prescription compared to the medium-dose group of modified Wenyang Sanjie Prescription， and IL-4， IL-6， IL-10， spleen index， thymus index， tumor weight， and tumor microvascular density were decreased， as well as HIF-1α， EGFR， and VEGF mRNA and protein levels （P<0.05）.ConclusionModified Wenyang Sanjie Prescription can significantly inhibit microangiogenesis， regulate Th1/Th2 balance， inhibit tumor growth， and significantly inhibit the progression of lung cancer in mice.关键词：modified Wenyang Sanjie prescription;lung cancer;T helper cell （Th）1/Th2 balance;tumor microangiogenesis;inflammatory factor;epidermal growth factor receptor5|0|0更新时间：2025-07-15
- “最新研究揭示开玄解毒核心方治疗银屑病的有效成分和作用机制，为银屑病治疗提供新思路。”摘要：ObjectiveTo identify the active constituents of Kaixuan Jiedu Core prescription （KXJD） and investigate its effective components and therapeutic targets in the treatment of common psoriasis.MethodsUltra-performance liquid chromatography-high resolution mass spectrometry （UPLC-Q-TOF MS） was used to identify and analyze the chemical components of KXJD and its blood-entering chemical components. The chemical components of the single decoction were further identified to clarify the source of the chemical components in KXJD. Then， all identified blood-entering compounds were screened for effective active ingredients through the Swiss ADME database. The active ingredient targets of KXJD were searched on the Swiss Target Prediction platform. The targets of common psoriasis were searched in OMIM， GEO， GeneCards， and DISGNET databases to obtain drug-disease intersection targets. The protein-protein interaction network of intersection targets was constructed using STRING， and then the core blood-entering component-intersection target network of KXJD was constructed. The core target proteins in the network were selected for molecular docking with the core components of KXJD. Small molecules and proteins were pretreated， and appropriate docking sites were selected. AutoDock Vina software was used for continuous local search and repeated iterations to find molecular docking conformations. PyMOL software and LigPlot+ software were used for analysis and visualization. Subsequently， the verification was carried out through animal experiments. SPF-grade male C57 mice were randomly divided into a blank group， a model group， a positive drug methotrexate group， and a KXJD group. In the model group， the methotrexate group， and the KXJD group， imiquimod was applied to the backs of the mice for modeling. The blank group and the model group were administered normal saline by gavage， while the methotrexate group and the KXJD group were respectively administered 1 mg·kg-1 suspension and 30.42 g·kg-1 decoction of traditional Chinese medicine by gavage. Five days after administration， the mice were sacrificed， and samples were collected. Hematoxylin-eosin （HE） staining was used to observe the skin tissue samples of mice， and the effect of KXJD on the pathological manifestations of psoriasis mice was analyzed. The expression areas of tumor necrosis factor-α （TNF-α）， cyclooxygenase 2 （PTGS2）， interleukin （IL）-1β， and IL-6 in the skin tissue of mice were detected by immunohistochemistry （IHC）. The mRNA expressions of TNF-α， IL-1β， IL-6， and PTGS2 in the skin tissue of mice were detected by real-time fluorescent quantitative polymerase chain reaction （Real-time PCR）.ResultsIn this study， 208 compounds in KXJD were identified by UPLC-Q-TOF MS， and 44 compounds were detected in serum， including 28 prototype components and 16 metabolites. 12 blood-entering active components were screened， and 1 153 active component targets and 1 076 psoriasis-related targets were identified. Eighty-five targets in the intersection set were drug-disease common targets. PPI network analysis showed that TNF-α， IL-1β， IL-6， PTGS2， and ALB were the key target proteins. The results of molecular docking showed that the binding ability of （+）-hexylphenylglycolic acid to key target proteins such as PTGS2 was stable， and PTGS2 showed high stability with a variety of core compounds. Compared with those in the blank group， the stratum corneum and epidermis in the model group mice were significantly thickened， and the pathological Baker score of the mice's skin in the model group was significantly higher than that of the blank group （P<0.01）. The expression areas of PTGS2， TNF-α， IL-1β， and IL-6 proteins in the skin tissue of the model group mice were significantly increased （P<0.01）， and the expression levels of PTGS2， TNF-α， IL-1β， and IL-6 mRNA in the skin tissue of the model group mice were significantly elevated （P<0.01）. Compared with the model group， the pathological Baker scores of the methotrexate group and the KXJD group were both decreased （P<0.01）， and the expression areas in the skin tissue of the methotrexate group and the KXJD group were significantly reduced （P<0.05， P<0.01）. The expression levels of PTGS2， TNF-α， IL-1β， and IL-6 mRNA in the skin tissue of the methotrexate group and the KXJD group were significantly decreased （P<0.01）.ConclusionKXJD can effectively improve the skin lesions of psoriasis model mice and reduce the expression of TNF-α， IL-1β， IL-6， and PTGS2 in the skin tissue of psoriasis model mice. PTGS2 has a stable binding ability with a variety of compounds in the decoction， which may be a key target for the treatment of psoriasis. The compound （+）-hexylphenylglycolic acid may play a key role.关键词：Kaixuan Jiedu;cyclooxygenase 2 （PTGS2）;psoriasis;network pharmacology;traditional Chinese medicine （TCM） compound5|0|0更新时间：2025-07-15
- “新绿原酸抑制银屑病细胞增殖和炎症，通过靶向HSP90调控NF-κB/NLRP3信号通路。”摘要：ObjectiveTo investigate the target proteins directly bound by neochlorogenic acid （NA） and the molecular mechanisms that ameliorate the proliferation and inflammatory response of psoriatic keratinocytes.MethodsM5-induced HaCaT cells were used as a psoriatic keratinocyte proliferation and inflammatory cell model. The synthesized NA probe （NA-P） and NA prodrug were first evaluated for cell viability using a cell proliferation/toxicity assay kit-8（CCK-8）. The potency of NA and NA-P was evaluated in the safe concentration range， and the effects of 0-100 μmol·L-1 NA and probe on M5-induced proliferation of HaCaT cells were detected using CCK8. The effects of 20， 40， and 80 μmol·L-1 NA and 80 μmol·L-1 NA probe on the expression of tumor necrosis factor-α （TNF-α）， interleukin-1β （IL-1β）， interleukin-23 （IL-23）， and interleukin-17A （IL-17A） inflammatory factors were detected by enzyme-linked immunosorbent assay （ELISA）， and real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） was used to measure the effects of NA on the mRNA expression of keratin 16 （K16） in HaCaT cells， S100 calcium-binding protein A9 （S100A9）， S100 calcium-binding protein A7 （S100A7）， IL-6， IL-17A， and chemokine 1 （CXCL1）. In vitro fluorescence labeling and competition experiments using NA probes were performed， and target protein angling and analysis using pull-down experiments combined with liquid chromatography-mass spectrometry （Pull-down/LC-MS/MS） were conducted. Target validation was performed using pull-down experiments combined with protein immunoblotting （Pull down-WB）， cellular heat transfer analysis combined with protein immunoblotting （CETSA-WB） experiments， and molecular docking. Finally， Real-time PCR was utilized to detect the effects of 20， 40， 80 μmol·L-1 NA and 80 μmol·L-1 NA probe on the mRNA expression of IL-1β， nucleotide-binding oligomeric structural domain-like receptor protein 3 （NLRP3）， apoptosis-associated speckled-like protein （ASC）， and cysteine protease-1 （Caspase-1） in HaCaT cells. Protein immunoblotting （Western blot） was used to detect the effects of phosphorylated p65 （p-p65）， p65， phosphorylated human nuclear factor-κB inhibitory protein α （p-IκBα）， human nuclear factor κB inhibitory protein α （IκBα）， and heat shock protein 90 （HSP90） expression.ResultsIn the 200 μmol·L-1 safe concentration range， HaCaT cell proliferation， increased expression of TNF-α， IL-1β， IL-23， and IL-17A inflammatory factors， and increased mRNA expression of K16， S100A9， S100A7， IL-6， IL-17A， and CXCL1 were observed in the M5 group compared with the blank group. Cell proliferation in 5-100 μmol·L-1 NA and NA-P groups was inhibited， and the expression of TNF-α， IL-1β， IL-23， and IL-17A inflammatory factors was decreased in the NA-L， NA-M， NA-H， and NA-P-H groups. The mRNA expression of K16， S100A9， S100A7， IL-6， IL-17A， and CXCL1 was decreased （P<0.05）. High-confidence targets were screened for HSP90 protein by Pull-down/LC-MS/MS using 200 μmol·L-1 NA competing with 100 μmol·L-1 NA-P. Compared with that in the blank group， the mRNA expression of NLRP3， IL-1β， ASC， and Caspase-1， as well as the expression of p-p65/p65， p-IκBα/IκBα， and HSP90 protein， were increased in HaCaT cells in the M5 group （P<0.05）. Compared with that in the M5 group， the mRNA expression of NLRP3， IL-1β， ASC， and Caspase-1 of cells in the NA-L group， the NA-M group， the NA-H group， and the NA-P-H group was decreased （P<0.05）. p-p65/p65 and p-IκBα/IκBα were decreased in the NA-M and NA-H groups （P<0.05）， and there was no change in HSP90 protein. Pull down-WB showed that NA could directly target HSP90 protein， and NA binding to HSP90 protein enhanced its thermal stability. Molecular docking of NA with HSP90 family proteins HSP90AA1， HSP90B1， and HSP90AB1 all resulted in highly stable binding.ConclusionNA can inhibit the proliferation and inflammatory response of psoriatic keratinocytes by a mechanism that may be achieved by targeting HSP90 to modulate the NF-κB/NLRP3 signaling pathway.关键词：neochlorogenic acid;HSP90;nuclear factor-κB/nucleotide-binding oligomeric structural domain-like receptor protein 3（NF-κB/NLRP3） signaling pathway;proliferation of psoriatic keratinocyte;inflammation7|0|0更新时间：2025-07-15
- “在抗癌领域，黄芪-莪术药对显示出显著的抗肿瘤活性，其机制涉及抑制肿瘤细胞增殖、促进凋亡自噬、抗侵袭转移等，为临床应用和新药开发提供参考。”摘要：Malignant tumor is a serious and difficult disease threatening human health， which has a high morbidity and mortality rate worldwide. Traditional Chinese medicine has unique advantages in improving the therapeutic effect of malignant tumors and alleviating adverse reactions. Traditional Chinese medicine believes that Qi deficiency and blood stasis are important pathogeneses in the development of malignant tumors， and the method of supplementing Qi and activating blood is an effective strategy for treating malignant tumors. Astragali Radix， sweet in taste and warm in nature， has effects of tonifying Qi and rising Yang， strengthening the exterior and reducing sweat， promoting fluid and nourishing blood. Curcumae Rhizoma， acrid and bitter in taste and warm in nature， has the effects of promoting Qi and breaking blood stasis， eliminating mass， and relieving pain. Astragali Radix-Curcumae Rhizoma， as the classic herb pair of invigorating Qi and activating blood， has a clear effect on inhibiting tumor growth and metastasis. Studies have shown that Astragali Radix-Curcumae Rhizoma contains astragalus polysaccharide， astragaloside， calycosin， formononetin， curcumin， β-elemene， curcumenol， curcumenone， curcumendione， gemacrone， and other anti-tumor active ingredients. It can significantly inhibit the occurrence and development of liver cancer， colorectal cancer， gastric cancer， lung cancer， ovarian cancer， cervical cancer， breast cancer， and other cancers and has the advantages of superposition effect， synergistic complementarity， and increased dissolution compared with single herb and monomer of Chinese traditional herbs and has been widely valued in the field of TCM anti-cancer. Its anti-tumor mechanism includes inhibition of tumor cell proliferation， promotion of tumor cell apoptosis and autophagy， anti-invasion and metastasis， regulation of immune function， and enhancement of anti-tumor drug sensitivity. By combining Chinese and foreign literature， the compatibility effect and anti-tumor mechanism of Astragali Radix-Curcumae Rhizoma were summarized， and then scientific compatibility of these two herbs was expounded， in order to provide a useful reference for clinical application and future research of Astragali Radix-Curcumae Rhizoma.关键词：Astragali Radix-Curcumae Rhizoma;herb pair;compatibility effect;mechanism of action;anti-tumor6|0|0更新时间：2025-07-15
- “据最新研究报道，中药复方调控NLRP3炎症小体治疗溃疡性结肠炎取得进展，为深入研究提供参考。”摘要：Ulcerative colitis （UC） is a refractory disease of the digestive system characterized by diverse etiologies， complex pathogenesis， a prolonged course， and frequent relapses. In recent years， the incidence of UC has been increasing annually， severely impairing patients' quality of life， posing a risk of malignant transformation that may threaten patients' lives， and resulting in a substantial medical burden. Traditional Chinese medicine （TCM） compound formulas， with their advantages of multi-component and multi-target actions， have become a new therapeutic option for UC. The NOD-like receptor pyrin domain-containing 3 （NLRP3） inflammasome is a core component of innate immunity， and its aberrant activation is closely associated with the onset and progression of UC， involving multiple processes such as inflammation and oxidative stress， and exhibiting crosstalk with pathways including nuclear factor-κB （NF-κB）， nuclear factor erythroid 2-related factor 2 （Nrf2）， and thioredoxin-interacting protein （TXNIP）. At present， NLRP3 has become one of the most intensely studied hotspots in UC-related research. Although increasing studies have focused on the regulation of the NLRP3 inflammasome by TCM compound formulas for UC treatment， challenges remain due to the complex pathogenesis of UC and the compositional diversity of TCM， hindering the realization of precision therapy. In this context， by reviewing literature from the past decade， this paper summarizes the activation process of NLRP3 and its relationship with UC， and elucidates the roles and mechanisms by which TCM compound formulas regulate the NLRP3 inflammasome and related signaling pathways， with a view to providing a reference for further research into the pathogenesis of UC， TCM treatment strategies， and their mechanisms of action.关键词：Chinese medicine compound formula;Nod-like receptor pyrin domain-containing 3（NLRP3） inflammasome;signaling pathway;ulcerative colitis5|16|0更新时间：2025-07-13
- “最新研究发现，绞股蓝皂苷通过经典/非经典铁死亡途径干预代谢相关脂肪性肝病小鼠肝脏脂质沉积。”摘要：ObjectiveTo explore the effect of gypenosides （GPs） on liver lipid deposition in metabolism-associated fatty liver disease （MAFLD） mice and its mechanism based on classical/non-classical ferroptosis.MethodsEight male C57BL/6 mice in a blank group and 32 male apolipoprotein E gene knockout （ApoE-/-） mice were randomly divided into a model group， a low-dose GPs （GPs-L） group， a high-dose GPs （GPs-H） group， and a simvastatin （SV） group. Starting from the second week， mice in the blank group were given a maintenance diet， and the other four groups were fed a high-fat diet daily. After eight weeks of feeding， mice in the GPs-L and GPs-H groups were given GPs of 1.49 mg·kg-1·d-1 and 2.97 mg·kg-1·d-1， respectively， and mice in the SV group were given simvastatin of 2.275 mg·kg-1·d-1. Mice in the blank group and the model group were given saline of equal volume by gavage for four weeks. The content of total cholesterol （TC）， triglyceride （TG）， low-density lipoprotein cholesterol （LDL-C）， high-density lipoprotein cholesterol （HDL-C）， alanine aminotransferase （ALT）， and aspartate aminotransferase （AST） in the serum of mice in each group was detected by an automatic biochemical analyzer. The level of non-esterified fatty acid （NEFA） and TG in the mouse liver was measured by the kit. The change in liver tissue structure and lipid deposition was observed by hematoxylin-eosin （HE） and oil red O staining. The levels of coenzyme Q10 （CoQ10）， glutathione （GSH）， malondialdehyde （MDA）， and Fe2+ in serum， as well as nicotinamide adenine dinucleotide phosphate ［NAD（P）H］ in the liver were detected by enzyme-linked immunosorbent assay （ELISA）. The expression of ferroptosis suppressor protein 1 （FSP1） in the liver of mice was observed by the immunohistochemical （IHC） method， and the expression of genes and proteins related to classical and non-classical ferroptosis pathways was analyzed by real-time polymerase chain reaction （Real-time PCR） and Wes automated protein expression analysis system.ResultsCompared with those in the blank group， the levels of TC， TG， LDL-C， ALT， and AST in serum and TG and NEFA in the liver in the model group were significantly increased， and the level of HDL-C in serum was significantly decreased （P<0.01）. The liver tissue structure changed， and there were fat vacuoles of different sizes and a large number of red lipid droplets， with obvious lipid deposition. The level of CoQ10 and GSH in serum and NADH in the liver were significantly decreased， while the level of MDA and Fe2+ in serum was significantly increased （P<0.01）. The mRNA and protein expressions of cystine/glutamate transporter （xCT/SLC7A11）， glutathione peroxidase （GPX4）， p62， nuclear factor E2-related factor 2 （Nrf2）， and FSP1 were significantly decreased， and the mRNA and protein expressions of tumor antigen （p53）， spermidine/spermine N1-acetyltransferase 1 （SAT1）， arachidonate 15-lipoxygenase （ALOX15）， and Kelch-like epichlorohydrin-associated protein-1 （Keap1） were significantly increased （P<0.01）. Compared with those in the model group， the level of TC， TG， LDL-C， ALT， and AST in serum and TG and NEFA in the liver of mice in the GPs-L， GPs-H， and SV groups were decreased， while the level of HDL-C in serum was significantly increased （P<0.05， P<0.01）. The liver tissue structure and lipid deposition were improved. The levels of CoQ10 and GSH in serum and NADH in the liver were significantly increased， while the levels of MDA and Fe2+ in serum were significantly decreased （P<0.05， P<0.01）. The mRNA and protein expressions of xCT， GPX4， p62， Nrf2， and FSP1 were significantly increased， while the mRNA and protein expressions of p53， SAT1， ALOX15， and Keap1 were significantly decreased （P<0.05， P<0.01）.ConclusionGPs can interfere with liver lipid deposition in MAFLD mice through classical/non-classical ferroptosis pathways.关键词：metabolism-related fatty liver disease;liver lipid deposition;classical/non-classical ferroptosis pathway;gypenoside5|6|0更新时间：2025-07-13
- “宁夏海原小茴香产地鉴别模型研究取得新进展，通过GC-IMS技术、PLS-DA分析及VIP值筛选，成功构建了稳定且预测能力强的鉴别模型，为海原小茴香的道地药材及国家地理标志性农产品保护提供新的技术力量。”摘要：ObjectiveTo establish a geographical origin identification model for Foeniculi Fructus from Haiyuan， providing a new technical reference for the protection of Haiyuan's geo-authentic medicinal materials and its designation as a national geographical indication agricultural product.MethodsSamples of Foeniculi Fructus were collected from eight producing areas， including Minqin （Gansu）， Bozhou （Anhui）， Qingdao （Shandong）， Dezhou （Shandong）， Urumqi （Xinjiang）， Nujiang （Yunnan）， Gutuo （Inner Mongolia）， and Haiyuan （Ningxia）. Gas chromatography-ion mobility spectrometry （GC-IMS） was used to detect the volatile organic compounds （VOCs） in samples from these geographic origins. VOCs were qualitatively analyzed through dual matching with the National Institute of Standards and Technology （NIST） mass spectral database and the IMS drift time database. Using the Reporter module and Gallery Plot visualization tools within the LAV analytical platform， VOC fingerprint profiles characterizing geographic origins were constructed. A non-targeted analytical strategy was adopted， and 97 VOCs detected via GC-IMS were subjected to principal component analysis （PCA） and partial least squares discriminant analysis （PLS-DA） based on their differential distribution patterns to construct an origin identification model for Foeniculi Fructus from Haiyuan region. Key discriminative markers were screened using variable importance in projection （VIP） values greater than 1.ResultsA total of 97 VOCs were identified， including alcohols， aldehydes， ketones， esters， organic acids， terpenoids， ethers， alkenes， and benzenes. The PLS-DA model， based on VOCs data obtained by GC-IMS， effectively distinguished Foeniculi Fructus in Haiyuan region from those of other origins. During cross-validation， the model achieved a prediction parameter （Q2） of 0.976 and a goodness-of-fit parameter （R2） of 0.936， with no overfitting observed in permutation testing. Twelve key flavor markers with VIP > 1 were identified as characteristic indicators of Haiyuan origin.ConclusionA stable and highly predictive origin identification model for Foeniculi Fructus from Haiyuan was successfully established using GC-IMS technology， PLS-DA， and VIP-based marker screening. This model provides a novel technical strategy for accurately distinguishing Foeniculi Fructus in Haiyuan region from other regional varieties and offers new technical support for its protection as a geo-authentic medicinal material and a nationally designated geographical indication agricultural product in China.关键词：gas chromatography-ion mobility spectrometry technology;volatile organic compounds;Foeniculi Fructus from Haiyuan;chemometric methods;geographical origin discrimination model5|11|0更新时间：2025-07-13
- “最新研究揭示药用琥珀鉴别特征，为质量控制与真伪鉴定提供科学依据。”摘要：ObjectiveTo systematically investigate the identification characteristics of medicinal amber， elucidating its microscopic features， crystal structural properties， and elemental composition， thereby providing a scientific foundation for quality control and authenticity verification.MethodsThirty-nine batches of amber samples were collected and analyzed through integrated techniques including morphological analysis， microscopic identification， powder X-ray diffraction （XRD）， Raman spectroscopy， Fourier-transform infrared （FTIR） spectroscopy， and inductively coupled plasma mass spectrometry （ICP-MS） to evaluate their morphological attributes， phase composition， molecular vibrational modes， and trace element profiles. Among them， the XRD experiment used Cu Kα radiation （λ=1.5406 Å）， with a scanning angle range of 10° to 70° （2θ） and a step size of 0.02°； the Raman spectroscopy experiment employed a 785 nm laser， with a spectral measurement range of 3 400 to 50 cm-¹， a laser power of 300 mW， a laser intensity of 30%， and a scanning time of 100 to 1 000 ms； the infrared spectroscopy experiment used a carbon-sulfur lamp， with a scanning range of 4 000 to 500 cm-¹， a resolution of 4 cm-¹， and 3 scans； the ICP-MS experiment utilized an RF power of 1.2 kW， a double-pass cyclonic spray chamber， a sample introduction system flow rate of 0.7-1.0 L·min-1， and an auxiliary gas flow of 0.2 L·min-1.ResultsUnder orthogonal polarized light microscopy， medicinal amber exhibited an isotropic homogeneous structure， with partial samples containing inorganic impurities such as AsS and SiO₂. FTIR spectra revealed characteristic absorption peaks at 2 932-2 939 cm⁻¹ （C-H stretching vibrations）， 1 705-1 728 cm-¹ （C=O stretching vibrations）， and 880-887 cm-¹ （C=C deformation vibrations）， confirming the oxidative polymerization of terpenoid resin. Raman spectroscopy further identified distinctive peaks at 2 925 cm-¹， 2 870 cm-¹ （saturated C-H stretching）， and 1 648 cm-¹ （C=C stretching）， consistent with the structural features of oxidized-polymerized resin. ICP-MS analysis demonstrated that S， Al， Si， Fe， Na， and Ca were the predominant trace elements in medicinal amber.ConclusionThis study comprehensively evaluated medicinal amber's morphological， crystallographic， molecular， and elemental characteristics through multimodal analytical techniques. The findings establish a robust framework for establishing quality standards for medicinal amber and distinguishing it from synthetic resin imitations， offering critical data support for pharmacopeial revisions.关键词：Medicinal amber;Multispectral analysis;Elemental profile;quality evaluation4|13|0更新时间：2025-07-13
- “最新研究发现，PI3K/Akt信号通路在哮喘病理进展中发挥关键作用，中医药干预该通路有望为哮喘治疗提供新思路。”摘要：Asthma is a chronic inflammatory respiratory disease involving multiple cells and cellular components， characterized by recurrent episodes of wheezing， shortness of breath， chest tightness， and coughing， significantly impacting patients' quality of life. The phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt） signaling pathway serves as a crucial hub in intracellular signaling， regulating cell growth， proliferation， survival， metabolism， and various pathophysiological processes. Its role in the pathological progression of asthma is profound， evident in promoting airway inflammation， mediating epithelial mesenchymal transformation， accelerating airway remodeling， regulating cell autophagy， inducing mucus hypersecretion， and influencing immune response balance. This study analyzes potential molecular targets of the PI3K/Akt pathway， identifying activators such as cysteine protease inhibitor Cystatin SN （CST1）， Found in Inflammatory Zones-1 （FIZZ1）， and Free Fatty Acid Receptor 1 （FFAR1）. Additionally， inhibitors such as human β-defensin 3 （hBD-3）， desintegrins， and metalloproteinase-33 （ADAM33） are examined as markers of airway inflammation and potential pathway inhibitors. Notably， interleukin-27 （IL-27） is also discussed. The paper initially unveils the potential molecular mechanisms of Traditional Chinese Medicine （TCM） in asthma intervention. The authors systematically summarize the efficacy and specific mechanisms of TCM monomers， combinations， and external treatments in regulating the PI3K/Akt signaling pathway through literature review and analysis. This aims to establish a robust foundation for the widespread application and advanced development of TCM in asthma treatment， offering innovative insights for clinical research and drug development in asthma management.关键词：traditional Chinese medicine;asthma;phosphatidylinositol 3-kinase（PI3K）/protein kinase B（Akt） signaling pathway;airway inflammation;airway remodeling;potential targets;research progress9|16|0更新时间：2025-07-11
- “最新研究揭示鸡骨草及毛鸡骨草的历史沿革和药用价值，为传统名方开发提供科学依据。”摘要：This article systematically analyzes the historical evolution of the name， origin， academic name， medicinal parts， origin， harvesting， processing and processing of Abri Herba and Abri Mollis Herba by referring to the herbal medicine， medical books， prescription books and other documents of the past dynasties， combined with the relevant modern research materials. Carding and textual research provide a basis for the development of classic prescriptions containing this type of medicinal materials. According to the verification， Abri Herba was first recorded in Lingnan Caiyaolu， with other names such as HuangTouCao and XiYeLongLinCao. It originates from the dried whole plant of Abrus cantoniensis， a Fabaceae plant， which can be used medicinally except for its fruits. Currently， this species is mainly distributed in GuangDong and GuangXi， and also found in HuNan and Thailand； it can be harvested throughout the year， with spring and autumn being the main seasons. The roots， stems， and leaves can be used for medicinal purposes， but the pods are toxic and must be removed. After harvesting， impurities and pods are removed， and it is dried and processed for medicinal use. Abri Herba has a sweet and slightly bitter taste， is cool in nature， and is associated with the liver and stomach meridians； it is used for clearing heat and dampness， dispersing blood stasis， and relieving pain； and is mainly used to treat jaundice-type hepatitis， stomach pain， rheumatic bone pain， contusion and ecchymosis pain， and mastitis.Abri Mollis Herba was first recorded in the 1982 edition of Zhongyaozhi， used in conjunction with A. cantoniensis. It was listed in Xinhua Bencao Gangyao in 1988. Other monographs also list it as a single herb， with its aliases and efficacy， while some other herbs use it as a local habitual product or confused product of Abri Herba. with other names such as DaYeJiGuCao， QingTingTeng， and MaoXangShi. It comes from the dried whole herb of Abrus molliswithout pods， mainly produced in GuangXi and GuangDong， and occasionally found in Hong Kong， HaiNan， and FuJian. The collection and processing are similar to Abri Herba； after harvesting， impurities and pods are removed， and it is dried and cut for medicinal use. Abri Mollis Herba has a sweet and light taste， is cool in nature， and is associated with the liver and stomach meridians， with effects of clearing heat and detoxifying， and promoting dampness； it is mainly used to treat infectious hepatitis， mastitis， furuncles， burns and scalds， and pediatric malnutrition. Based on the research， A. mollis was first recorded to be used as a medicine in the same base as A. cantoniensis， and as plants of the same genus， have very similar morphological characteristics， and their medicinal parts， collection and processing， properties and flavors， and meridian affiliations are consistent. And A. mollis in the folk have as Abri Herba use， which has been used for a long time and is now dominated by the cultivation of A. mollis. so it is recommended that the Chinese Pharmacopoeia will be A. mollis can be with the inclusion of Abri Herba into the same medicinal herbs base material， and it is recommended that in classical prescriptions refered to Jiguccao can use A. cantoniensis and A. mollis as the sources of the herbs to be used together in the medicine. Refered to MaoJiguccao can use A. mollis as the sources of the herbs to be used respectively. Prepare according to the preparation requirements specified in the original prescription， and if there are no requirements， use the raw product for medicine.关键词：famous classical formulas;Abri Herba;Abri Mollis Herba;origin;scientific name;medicinal parts;processing;herbal textual research4|18|0更新时间：2025-07-11
- “最新研究发现，现代工艺与古法工艺制备的百合地黄汤质量标志物含量相当，药效作用一致，为百合地黄汤新药开发提供科学依据。”摘要：ObjectiveBased on analysis and chronic unpredictable mild stress（CUMS）， the depression model was established to evaluate the quality components and pharmacological effects of Baihe Dihuangtang prepared by different techniques.MethodsHPLC was used to establish the characteristic profiles of Baihe Dihuangtang， The content of Q-Marker and the yield of Baihe Dihuangtang prepared by ancient and modern techniques were measured separately；Seventy C57BL/6J mice were randomized into normal， model， fluoxetine（3 mg·kg-1）， low-and high-dose（6. 5， 26 g·kg-1， respectively）Baihe Dihuangtang by ancient techniques， and low-and high-dose（6. 5， 26 g·kg-1， respectively）Baihe Dihuangtang by modern techniques groups， with 10 mice in each group. Other groups except the normal group was induced by CUMS method in the modeling group to induce depression. After 28 days of stimulation， administration groups were given relevant medicine intragastrically， once a day， for 21 consecutive days. The behavioral indicators such as changes in body mass， tail suspension test， and open field test were detected in each group；Enzyme-linked immunosorbent assay（ELISA）was employed to determine the content of tumor necrosis factorα（TNF-α）， interleukin1β（IL-1β）and interleukin6（IL-6）.ResultsThe similarity of Baihe Dihuangtang characteristic profiles was>0. 999， and 23 common peaks were calibrated， nine chromatographic peaks were identified. The Q-Marker content in Baihe Dihuangtang prepared by ancient and modern techniques is equivalent， with average paste yields of 17. 20%and 17. 07%， respectively. Compared with normal group， the model group showed increased immobility time in the tail suspension test（P<0. 01）， and reduced residence time in the central area of the open field and the total movement distance（P<0. 01）， In addition， the modeling elevated the level IL-1β， IL-6， and TNF-α in the serum（P<0. 01）. Compared with the model group， the behavioral indicators of mice in the Baihe Dihuangtang treatment group were significantly improved in terms of tail suspension time and open field exercise（P<0. 05， P<0. 01）， and the levels of IL-1β， IL-6， and TNF-α in serum were significantly reduced（P<0. 05， P<0. 01）. Both Baihe Dihuangtang treatments had antidepressant effects， and there was no significant difference in improving depressive symptoms between two treatments.ConclusionThe Q-Marker of Baihe Dihuangtang prepared by modern and ancient methods are equivalent in content， and the pharmacological effects are consistent. This indicates that dried lilies can replace fresh lilies in the preparation of Baihe Dihuangtang， providing a scientific basis for the development of new drugs for Baihe Dihuangtang and a reference for its rational application and clinical use.关键词：Baihe Dihuangtang;quality markers;characteristic mapping;pharmacological research;chronic unpredictable mild stress（CUMS）;antidepressant5|13|0更新时间：2025-07-11
- “紫苏梗质量差异研究取得进展，专家构建了熵权-TOPSIS模型，为紫苏梗质量评价提供解决方案。”摘要：ObjectiveBased on the content of volatile and nonvolatile components in Perillae Caulis， this article explored differential components of Perillae Cauliss with different characters and origins， and screened the premium Perillae Cauliss.MethodsThis study collected 32 batches of Perillae Cauliss from 12 producing areas recording their diameters， epidermis colors and producing areas. The contents of total flavonoids， total phenols， volatile oils， 5 active components and 84 volatile components in 32 batches of Perillae Cauliss were quantitatively or semi-quantitatively determined using microplate reader， UPLC-PDA and GC-MS. Then the component content difference was analyzed using PCA and nonparametric test. Based on the weight of indices from PCA model， TOPSIS model was constructed to evaluate the quality of Perillae Cauliss with different characters and origins.ResultsThere were significant differences in the composition of Perillae Cauliss with different diameters， epidermis colors and producing areas， with 9 differential components screened， including 6 key indices and 3 volatile compounds， namely total flavonoids， total phenols， caffeic acid， scutellarin， rosmarinic acid， luteolin； caryophyllene oxide， （-）-humulene epoxide Ⅱ， 14-hydroxycaryophyllene. The content of 6 index components was higher in Perillae Cauliss with small diameter， purple brown skin and southern origins while the content of 3 volatile components was higher in Perillae Cauliss with large diameter， dark brown skin and northern origins. A significant difference was shown in the model scores of different diameters， skin colors and origins （P < 0.01）. Perillae Cauliss with small diameter， purple-brown skin， from southern area especially Guangdong， had a high score.ConclusionInfluenced by the diameter， skin color and producing area， the chemical composition and content of Perillae Cauliss varied. Perillae Cauliss showing small diameter， owing purple-brown skin， and originating from Guangdong Province were of higher-quality due to their higher content of 8 key indices.关键词：appearance;origin;entropy weight-technique for order preference by similarity to an ideal solution（TOPSIS）;chemometrics;quality difference;Perillae Caulis7|14|0更新时间：2025-07-11
- “最新研究发现，鼻腔给药联合新型递药系统，能有效提高中药成分脑内浓度，为中枢神经系统疾病治疗提供新策略。”摘要：Central nervous system （CNS） diseases are significantly hindered by their complex pathological mechanisms and the presence of the blood-brain barrier （BBB）， which limits the effectiveness of drug treatments. Traditional drug administration methods， such as oral administration， intravenous injection， and transdermal delivery， despite having certain advantages， all face the challenge that drugs struggle to effectively cross the BBB. Therefore， identifying drug delivery methods that can efficiently traverse the BBB is crucial. Nasal drug delivery， as a non-invasive route， offers the potential for targeted CNS delivery via three pathways： olfactory neurons， trigeminal neurons， and the bloodstream. This approach holds considerable promise in the treatment of CNS diseases. Compared to chemical drugs， traditional Chinese medicine （TCM） features multiple components， multiple targets， and fewer adverse effects， providing unique advantages in the treatment of CNS diseases. A substantial body of research has further confirmed that nasal delivery combined with novel drug delivery systems such as lipid nanoparticles， nanoparticles， nanoemulsions， and composite in situ gels can effectively load the active components of TCM， significantly enhancing drug concentration in the brain and offering new strategies for the treatment of CNS diseases. This article systematically reviews the current state of drug delivery for CNS diseases， delves into the characteristics of nasal drug delivery， and focuses on summarizing and analyzing the research progress of passive targeting， active targeting， and "guided" drug delivery strategies for the nasal-to-brain transport of TCM active components. The aim is to provide references and insights for the development of drugs for CNS diseases and the application of TCM in nasal-to-brain delivery.关键词：traditional Chinese medicine;nasal delivery;central nervous system diseases;blood-brain barrier;drug delivery system;guide medicine upward;research progress9|22|0更新时间：2025-07-11
- 摘要：“Liver being substantial Yin and functional Yang” maintain normal function of qi， blood and meridians. In clinical practice， it is often found that pan vascular lesions with atherosclerosis as the predominant pathologic change often co-occur with metabolic dysfunction-associated fatty liver disease（MAFLD）. MAFLD leads to increased risk and worse prognosis for many pan vascular diseases， including cardiovascular disease. Dysregulation of energy homeostasis disrupts the hepatic homeostasis of body use， and representative drugs to improve metabolism， such as metformin， sodium-glucose cotransporter 2 inhibitors， and glucagon-like peptide-1 agonists， not only have a clear cardiovascular benefit， potential improvement of MAFLD has also been demonstrated. The liver stores blood and the heart pumps blood， and liver diseases affect the heart， that's why the unsmoothness of vessels appears. So the treatment should from the standpoint of liver， restoring liver function， soothing the liver and nourish heart， activating blood and dredging meridian. It is of great significance to explore in depth the pathogenesis and treatment of pan vascular lesions caused by MAFLD， and to restore the energy homeostasis by adjusting the balance of liver yin and yang.关键词：liver being substantial Yin and functional Yang;metabolic dysfunction-associated fatty liver disease;panvasculopathy;energy metabolism;imbalance of Yin and Yang;atherosclerosis;traditional Chinese medicine pathogenesis1|0|0更新时间：2025-07-11
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